20080868 Ver 2_Biological Monitoring of Outfall SW-2_20160419

Ardaman & Associates, Inc.
Ceotechnical, Environmental and
Materials Consultants
April 19, 2016
File Number 10-10-0133E
Mr. John Kroske
Environmental Engineer
USEPA
RCRA Enforcement and Compliance Branch
Atlanta Federal Center
61 Forsyth Street, S.W.
Atlanta, Georgia 30303-8909
Subject: Report of Biological Monitoring of the Pamlico River near Outfall SW-2, PCS
Phosphate — Aurora, NC, EPA ID No. NCD 041 519 364, RCRA Section 3013,
Docket No. RCRA-04-2008-4021, Aurora Phosphate Operations, PCS Phosphate
Company, Inc., Aurora, North Carolina
Dear Mr. Kroske:
Ardaman & Associates, Inc. (Ardaman) has completed the field and laboratory work associated
with the proposed biological monitoring of the Pamlico River near ouffall SW-2 at the PCS
l Phosphate Company, Inc., (PCS) — Aurora facility. This work effort was proposed by Ardaman in
our July 17, 2015 report entitled "Supplemental Addendum to SAR Work Plan Results".
The objective of the biological sampling is to discern differences, if any, in benthic
macroinvertebrate populations between the background area in the vicinity of Garrison Point and
the SW-2 outfall area
Background
As part of the RCRA 3013 sampling and analysis program for the subject facility, benthic sediment
sampling was performed at the facility's NPDES outfalls. Sediment samples were collected on
August 14 and August 18, 2009 at outfalls SW-1 and SW-2. Two samples were collected at each
location; one from 0 to 6 inches and one from 18 to 24 inches. The sediment sample obtained
from a depth of 0 to 6 inches at SW-2 had a measured cadmium concentration of 5.5 mg/kg,
which is above the threshold effects concentration of 1.0 mg/kg and also just above the probable
effects concentration of 5 mg/kg. Two additional samples collected on July 2, 2010 from the SW-
2 outfall indicated a measured concentration of 5.1 mg/kg for the sample from 0 to 6 inches and
5.5 mg/kg sample from 18 to 24 inches.
To determine the horizontal and vertical extent of the elevated cadmium concentrations, benthic
sediment samples were collected at 10 distinct sampling locations in and around the SW-2 outfall
on May 13 and 14, 2014. In addition, sediment pore water was collected via a Push Point® sample
tube 1 foot below the river bottom at each of the 10 sediment sampling locations.
Analysis from the May 2014 sediment samples indicated sediment cadmium between 2.9 and 9.5
mg/kg. Dissolved cadmium results for all ten sediment pore water samples were less than the
method detection limit of 0.36 pg/L.

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations -2-
A fourth round of benthic sediment sampling was conducted in July 2015. The benthic sediment
survey was expanded to include eleven additional sites within the Pamlico River adjacent to the
SW-2 ouffall. In addition to the eleven sites near SW-2, three background sites located
approximately 1.2 miles north (upstream) of SW-2- and just below the confluence of Durham
Creek and the Pamlico River were sampled. Cadmium concentrations for the shallow samples
obtained near SW-2 were in the range of 4.51 mg/kg to 10.30 mg/kg. Cadmium concentrations
for the deeper samples were in the range of 4.04 mg/kg to 10.20 mg/kg. The cadmium
concentrations at the background sites were below the method detection limit.
Biological Sampling Program
A biological sampling plan was developed in response to data collected and reported in the July
17, 2015 Supplemental Addendum to SAR Work Plan Results. The biological sampling plan,
approved on November 12, 2015 included the sampling of benthic sediments, benthic
macroinvertebrates and cadmium analysis of benthic macroinvertebrate tissue at six sampling
locations, three at the background site and three near SW-2. Sampling locations are shown on
Figures 1 through 3. Sampling occurred on December 9 and 10, 2015.
In Situ Water Quality
Surface water quality data were collected at each of the six sampling locations. At each sampling
location, classic limnologic parameters such as Secchi disk depth, conductivity, dissolved oxygen,
oxygen reduction potential, pH and total depth were collected. To collect these data, a calibrated
YSI 556 hand held multi -meter was attached to the YSI 600 data sonde with an optical dissolved /
oxygen probe.
Upon reaching each sampling site, the sampling boat was eased into position to avoid disturbing
the bottom sediments, which were at depths of about two feet. As the boat reached the sampling
coordinates, the anchor was slowly lowered into place catching the boat and allowing the swing
of the boat position to be within several feet of the site coordinates.
After the boat was secured at the site, the Secchi disk depth was measured on the sunny side of
the skiff. Water quality parameters were then taken every half foot of depth. After the data were
collected, the YSI was secured and benthic sampling began.
Benthic Sediment Collection
Benthic sediments and macroinvertebrates were collected using a Stainless Steel Van Veen
dredge sampler with an approximate bottom area of 470 cm'. Samples were obtained using the
methods described in USEPA-841-R-09-003 National Coastal Condition Assessment Field
Operations Manual. Benthic sediment samples collected were at least 7 cm in depth. Sample
grabs that were less than 7 cm in depth were discarded. Benthic samples that were protruding
out of the sample or not level within the grab bucket were not collected nor were residual samples
that remained if the dredge did not close properly allowing sediments to spill out during retrieval.
The Van Veen dredge was cleaned of benthic sediments between each location with a brush and
local river water. After each sampling day the Van Veen dredge was washed and allowed to dry.

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations
Macroinvertebrate Identification.
-3-
Benthic macroinvertebrate samples for identification were the first samples to be collected after
the water quality profile data had been collected and the equipment stowed. A clean Van Veen
dredge was used to collect benthic macroinvertebrates for taxa identification. The one drop
method was used to minimize any bias from multiple sample sets for taxa living in benthic
sediments. Similar water depths were used for each site during the collection of benthic
macroinvertebrate taxa samples.
Once the Van Veen dredge was recovered, benthic sediments and any other debris was gently
washed from the outside of the dredge and back into the Pamlico River. Benthic sediments
collected inside the Van Veen dredge were spilled out into a No. 30 screen wash bucket. Any
residual benthic sediments within the dredge were gently washed out into the No. 30 screen wash
bucket using a squeeze bottle filled with distilled water.
Benthic sediments were processed in the wash bucket allowing particles smaller than the. Number
30 sieve (0.6 mm) to pass through the screen and back into the Pamlico River. The particles and
organisms that did not pass during washing were collected in a small pan. Any organisms that
were caught within the wash bucket were field picked and placed in the small pan with the rest of
the benthic sediment taxa sample.
After processing the benthic sediments and placing them in the sampling pan, the benthic
macroinvertebrate and minimal benthic sediments were decanted and placed in a square, 300
mL, HDPE sample bottle. The sample bottle containing the benthic sample was then filled to the
shoulders with a 10% buffered formalin solution to preserve the benthic macroinvertebrates for
later identification.
An identification tag indicating sample identification, date, and time was placed in the sample
bottle prior to sealing. The benthic sample bottle was appropriately labeled and sealed, then
placed in a cooler for storage and shipment to the biological laboratory for identification to the
lowest practical taxon. On December 15, 2015, six benthic macroinvertebrate samples were
shipped from Ardaman and Associates, Inc. arriving at Aquatic Resources Center in Nashville,
Tennessee. Samples received on December 18, 2015 were said to have arrived in good
condition.
The six samples received on December 18, 2015 were washed of preservative and excess
detritus using a Number 35 sieve (0.5 mm). Samples were subsampled using a modified Caton
(1991) method. This process consists of dividing a given sample into equal portions using a
specified subsample device (graduated sampling pan), then sorting at least four randomly
selected grids to obtain 200 ± 20 percent organisms. The benthic organisms were removed from
the sample using a 10X power dissecting microscope. The organisms were placed in major
groupings (e.g., amphipods, worms, snails) into glass vials containing 70 percent ethyl alcohol.
Each vial was labeled with information such as date of collection, location, specific sampling
identification, name of taxonomic group and number of organisms. The residuals from the sorted
portion was preserved separately from the portion that was not sorted. Organisms were identified
using either dissecting or compound microscope. Most organisms were identified to the generic
level, unless the specimens were too small or damaged to allow identification at this level.
Identifications were completed and recorded on laboratory bench sheets. When Identification
was completed, the data were entered into Excel spreadsheet and checked for accuracy.
l

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations
Macroinvertebrate Cadmium Analysis.
-4-
Benthic macroinvertebrates obtained for cadmium tissue analysis were collected similarly to the
benthic macroinvertebrate taxa sample. Three drops of the Van Veen dredge were used to collect
what was perceived as enough tissue sample to run the cadmium tissue analysis. Three samples
collected with the Van Veen dredge were processed through the Number 30 screen wash bucket
and poured into a translucent plastic sorting tray. Distilled water or forceps were used to dislodge
any clinging taxa from the screen or the inside of the wash bucket.
Once in the sorting tray, all benthic macroinvertebrates were collected and placed in a smaller
container that was provided by the analytical laboratory as part of the ultra -trace metals kit. The
sample containing the picked benthic macroinvertebrates was decanted and rinsed with distilled
water and decanted for a second time.
A clean hands/dirty hands protocol was used to collect the benthic macroinvertebrate cadmium
samples from the secondary container to the ultra -trace sampling kit. After the samples were
collected and identification affixed to the outer sample bag, the samples were placed on dry ice
for immediate freezing. Samples for total cadmium tissue analysis were maintained in a cooler
filed with dry ice and maintained frozen for the duration.
Six benthic macroinvertebrate samples for cadmium tissue analysis were shipped to Brooks
Applied Labs (BAL) in Bothell, Washington on December 15, 2015 arriving in good condition on
December 16, 2015. The samples were received at -32 'C. r
Samples received on December 16, 2015 were received, prepared, analyzed and stored `
according to Brooks Applied Labs (BAL) SOP and EPA methodologies for analysis of total
cadmium. BAL is accredited by the National Environmental Laboratory Accreditation Program
(NELAP) through the state of Florida Department of Health, Bureau of Laboratories (E87982) and
is certified to perform total cadmium analysis.
During laboratory processing, benthic samples were defrosted and the hard shell of bivalves were
removed prior to sample hominization and trace metals analysis. Each of the six samples were
analyzed for total cadmium, using method EPA 1638 Modified. Trace metals were analyzed using
inductively coupled plasma triple quadrupole mass spectrometry (ICP-QQQ-MS). The ICP-QQQ-
MS uses advanced interference removal techniques to ensure accuracy of the sample results.
Benthic Sediment Analysis.
Benthic sediments obtained for grain size analysis were collected after the macroinvertebrate taxa
and macroinvertebrate cadmium samples were collected and stowed. Care was taken to collect
the benthic sediment samples within a 2 meter orbit of the macroinvertebrate taxa and cadmium
analysis samples. Benthic samples for the grain size analysis were not collected in previously
dredged holes. One drop of the Van Veen dredge was needed to collect benthic sediments for
grain size analysis. After the Van Veen dredge was recovered, representative benthic sediments
were collected with a stainless steel spoon and placed in an appropriately labeled 1L
polypropylene bottle and placed in a cooler for storage.
Upon reaching the Ardaman and Associates, Inc. soils laboratory on December 18, 2015, the
benthic sediments were submitted for Water Content (ASTM Standard D-2216) and Sieve
Analysis (ASTM Standard D-422).

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations -5-
( Sediments were placed in numbered tare containers, weighed, and placed in a 110 °C oven over
night. Samples were then weighed and the moisture content calculated. Next, the samples were
inundated with a 5 percent sodium hexametaphosphate solution and washed over a Number 200
sieve (0.076 mm) to remove the minus 200 particles. After washing, the samples were dried in a
110 °C oven overnight. The samples were re -weighed and placed in a standard sieve stack for
standard sieve analysis. Standard sieve analysis was performed and calculations of the percent
retained on each sieve was completed for each sieve screen.
Results
Surface Water Qualit
Sampling was split between two days with similar weather patterns and wave heights. The water
surface was calm. The flow did not appear to be significantly influenced by tidal currents or wind
patterns. Water column profiles were completed at each of the six sampling locations over the
two day sampling period at 0.50 ft intervals until the bottom sediments were encountered.
Surface water quality profile data are presented in Table 1. Secchi disk depth or the relative depth
of natural light penetration was to the bottom depth at each location. Secchi disk depth taken on
December 9, 2016 indicated total depth ranged between 1.91 ft and 2.25 ft at Bio-2W and Bio-
2E. Secchi disk depth taken the following day had a range difference of 3.16 ft and 3.33 ft at Bio-
12 and Bio-16.
Water column pH ranged between 8.32 and 8.14 at the surface and 8.01 and 8.33 at the water -
sediment interface at the three background locations. Water column pH ranged between 8.11
and 8.27 at the surface and between 8.00 and 8.13 at the water -sediment interface at the three
SW-2 locations. These are within the range expected for brackish water.
Specific conductance was different between the two locations. Specific conductance at the
background sites ranged between 7.33 mS/cm and 8.26 mS/cm at the water surface and between
8.15 mS/cm and 8.43 mS/cm at the water -sediment surface. Specific conductance at SW-2
ranged between 10.13 mS/cm and 10.87 mS/cm for the water surface readings and between
12.85 mS/cm and 12.98 mS/cm at the water -sediment interface. The water at SW-2 was
approximately 60% more conductive than the water at the background sites.
Salinity was also different at the two sites. Salinity at the background sites ranged between 5.3
ppt and 6.2 ppt at the water surface and between 6.0 ppt and 6.3 ppt at the water -sediment
surface. Salinity at SW-2 ranged between 7.6 ppt and 8.3 ppt for the water surface readings and
between 9.8 ppt and 9.9 ppt at the water -sediment interface. The water at SW-2 was
approximately 60% more saline than the water at the background sites.
Dissolved Oxygen (DO) readings at the water surface at the background sites were between
12.18 mg/L and 12.37 mg/L. DO concentrations at the water -sediment interface were between
12.33 mg/L and 14.86 mg/L. DO readings at the water surface at SW-2 were between 10.80 mg/L
and 11.83 mg/L and at the water -sediment interface were between 10.14 mg/L and 12.65 mg/L.
Oxidation Reduction Potential (ORP) readings collected at the background location were between
143.70 my and 193.30 my at the surface and between 142.60 my and 189.30 my at the water -
sediment interface. ORP readings at the SW-2 location were between 96.70 my and 147.60 my
at the surface and between 103.00 my and 148.60 my at the water -sediment interface.

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations -6-
Water temperature was relatively stable, varying slightly between the two days at the monitoring
locations. Surface water column temperatures on December 9, 2015 range between 13.17 °C
and 12.62 °C. The water column temperature on the following day at the SW-2 site ranged
between 14.33 °C and 12.62 °C.
Benthic Sediment Analysis
The grain size distribution of the benthic sediments at the two sampling locations are presented
in Table 2. The laboratory data sheets are attached as Appendix A.
Table 2
Benthic Sediment Distribution
% Passing
US Standard
Sample Location
Grain Size
Sieve Size
Bio-2W
Bio-2
Bio-2E
Bio-12
Bio-13
Bio-16
No.4
100.0%
100.0%
100.0%
100.0%
100.0%
100.0%
Fine Gravel
No.10
99.7%
100.0%
100.0%
99.8%
100.0%
100.0%
Coarse Sand
No.20
99.7%
100.0%
100.0%
98.7%
99.7%
99.4%
Medium
No.40
96.1%
96.3%
96.3%
90.8%
96.1%
93.9%
sand
No.60
1 85.9%
86.5%
88.2%
63.6%
71.6%
66.10.
No. 100
41.4%
45.3%
51.6%
15.7%
19.9%
14.7%
Fine Sand
No.140
6.6%
9.3%
10.1%
2.6%
2.7%
2.3%
No.200
1.5%
1.5%
1.8%
0.7%
0.6%
0.6%
The sediments from the background location contain a higher percentage of finer sediments than
the sediments from SW-2. The percentage of particles passing the No. 100 sieve for the
background sediments is approximately 40% to 50%. The percentage of particles passing the
No. 100 sieve for the SW-2 sediments is approximately 15% and 20%.
Macroinvertebrate Identification
Benthic macroinvertebrates were collected using a single Van Veen dredge drop. All of the
samples collected were of similar volume. Each sample was washed through a No. 30 screen
wash bucket with all remaining benthic macroinvertebrates retained on the screen collected and
sent for taxonomic identification to the lowest practical taxon.
As part of the sample identification, the entire sample from a site is placed within a gridded sorting
pan with 16 equally sized grids. A random selected grid is chosen and all benthic
macroinvertebrates are counted and identified. If a count of 100 organisms is not reached then
a second grid is chosen and the benthic macroinvertebrates are added to the previous count.
This will continue until either a count of 100 organisms are reached or the 16 grids have been
exhausted.
As shown in Table 3, the background locations Bio-2W, Bio-2 and Bio-2E did not produce a 100
count from the samples submitted. All 16 grids were sorted and taxa identified and counted. Bio-
2W the most westerly and upstream Bio monitoring site reached a total count of 85 organisms.

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations -7-
The count for Bio-2 and Bio-2E were 40 and 39 organisms, respectively. Bio-2 had the lowest
diversity with only 4 taxa. A total of 7 taxa were identified at Bio-2W and Bio-2E. Approximately
50% of the total organisms identified at the background site were amphipods, approximately 35%
were bivalves, and the remainder were marine worms. A total of nine species and a total of 164
individuals were identified from 11,304 cm' of sediment.
As shown in Table 3, the sorting process for the SW-2 Bio locations were able to reach the 100
+/- individual count without exhausting the 16 grids within the sorting pan. Bio-12 needed 6 grids
to obtain a total of 103 individuals, Bio-13 needed 9 grids to obtain a total of 116 individuals, and
Bio-16 needed 6 grids to obtain 99 individuals. Approximately 80 to 90% of the total organisms
at the SW-2 site were bivalves. Less than 10% of the total organisms identified at SW-2 were
amphipods. The remaining 5 to 15% of the organisms were marine worms. Eleven species were
identified and a total of 318 individual taxa counted from the 12,717 cm' of sediments.
Species Richness, Shannon -Wiener Diversity and Species Evenness were computed for both
sites. The results are presented in Table 4. The SW-2 location had the higher species richness.
The background location had the higher Shannon -Weiner Index. Evenness was also higher at
the background location.
Bench sheets detailing the order and quantity of benthic macroinvertebrate species that were
identified by Aquatic Resources Center are attached as Appendix B.
Macroinvertebrate Cadmium Tissue Analysis.
Ultra -trace total cadmium analysis was completed on six benthic macroinvertebrate samples
collected from six different locations within the background and SW-2 sampling locations. The
background location Bio-2W, Bio-2 and Bio-2E were collected on December 9, 2015. The SW-2
locations Bio-12, Bio-13 and Bio-16 were collected on December 10, 2016.
All of the benthic macroinvertebrate samples collected for cadmium tissue analysis were placed
in ultra -trace metal sample bottle provide by the analytical laboratory and stored in a cooler filled
with dry ice until they were shipped and received at Brooks Applied Labs located in Bothell
Washington. The benthic macroinvertebrate samples for cadmium analysis were received on
December 16, 2015 at a recorded sample temperature of <-32 °C.
Benthic macroinvertebrate Mollusca species were shucked in processing to remove any hard
material that could interfere with the analysis. All samples were homogenized at BAL prior to
analysis.
The results of the cadmium tissue analyses are presented in Table 5. As a result of limited sample
volume, a matrix spike and matrix spike duplicate could not be performed on one native sample.
A separate method duplicate and matrix spike were prepared and reported.

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations
Table 5.
Benthic Macroinvertebrates Tissue Cadmium
Sample ID
Date Sampled
Cadmium
mg/kg
MDL
mg/kg
MRL
mg/kg
Bio-2W
12/9/2016
0.010
0.003
0.005
Bio-2
12/9/2016
0.023
0.002
0.005
Bio-2E
12/9/2016
B
0.008
0.005
0.01
Bio-12
12/10/2016
0.402
0.001
0.003
Bio-13
12/10/2016
M
0.347
0.001
0.002
Bio-16
12/10/2016
0.320
0.001
0.003
"B" Detected by the instrument, the result is greater than the MDL but less than the MRL.
"M' Duplicate precision (RPD) was not within acceptable criteria. Result is estimated.
-8 f
The full analytical report for ultra -trace macroinvertebrate cadmium analysis is attached as
Appendix C
Conclusions
• Benthic macroinvertebrates were collected at two separate locations in the nearshore waters
of the Pamlico River from previously established locations. The background location is
approximately 1.2 miles to the west and upstream of the SW72 NPDES outfall location.
• Surface water quality data document ample available dissolved oxygen at the temperatures
and salinity measured. Salinity and specific conductance were approximately 60% greater at
the outfall location than at the background location. Hydrogen ion activity (pH) was
approximately 8.2, which is within the range expected for brackish water.
• Sediment analyses indicate that there is a difference in benthic sediment habitat between the
two locations that may influence the biota found there. The sediment is finer at the background
location and the overlying water column is less brackish. Each macroinvertebrate species
develops specific survival characteristics for habitats they are located in and can have an
effect upon the density and diversity found within each substrate.
• Benthic macroinvertebrates were collected in near shore waters with a single drop of a Van
Veen dredge. There was a noted difference in the quantity and species found between the
two sets of samples.
• A total of nine species and a total of 164 individuals were identified from 11,304 cm' of
sediment obtained from the background site. Approximately 50% of the total organisms
identified at the background site were amphipods, approximately 35% were bivalves, and the
remainder were marine worms.
• A total of eleven species were identified and a total of 318 individual taxa counted from
approximately one-half of the 12,717 cm' of sediments collected at the SW-2 outfall location.
Approximately 80 to 90% of the organisms at the SW-2 site were bivalves. Less than 10% of
the organisms identified at SW-2 were amphipods. The remaining 5 to 15% of the organisms

Biological Monitoring Report
PCS Phosphate Company
Aurora Phosphate Operations -9-
were marine worms. Both the abundance and richness are greater at the SW-2 location. .
The background location had the higher diversity (Shannon -Weiner Index).
• The concentration of cadmium in the macroinvertebrate tissue was higher at the SW-2 location
where the sediment concentrations are higher.
If you have any questions concerning the above report, please contact the undersigned.
Very truly yours,
ARDAMAN & YbOCIATES, INC.
Yf. 6J6'v.�
n . Garlanger, Ph.D., P.E.
Senior Consultant
North Carolina License No. 9046
/Jg
T. Baker, PCS (w/encl.)
D. Winstead, PCS (w/encl.)
R. Smith, PCS (w/encl.)
J. DeMonte, PCS (w/encl.)
M. Deemer, Jones Day (w/encl.)

TABLE 1
SURFACE WATER QUALITY
r
Pamlico River, NC
Depth
pH
Temp
Conductivity
DO
ORP
Salinity`
December 9, 2015
FT
SU
'C
mS/cm
mg/L
my
ppt
N
O
m
0.50
8.14
13.17
7.33
12.18
151.20
5.3
1.00
8.18
12.97
7.39
13.01
151.60
5.4
1.50
8.27
13.01
7.95
13.11
152.30
5.9
2.00
8.29
12.77
8.43
14.86
152.70
6.3
O
m
0.50
8.24
12.90
7.98
12.37
143.70
5.9
1.00
8.28
12.90
7.90
12.80
141.80
5.8
1.50
8.30
12.80
7.83
12.93
141.80
5.8
2.00
8.01
13.00
8.15
14.10
142.60
6.0
N
6
m
0.50
8.32
12.62
8.26
12.32
193.90
6.2
1.00
8.32
12.66
8.31
12.35
191.50
6.2
1.50
8.33
12.70
8.34
12.34
189.50
6.2
2.00
8.33
12.72
8.37
1 12.33
189.30
6.2
calculation
Pamlico River, NC
December 10,2015
Depth
pH
Temp
Conductivity
DO
ORP
Salinity
FT
SU
C
mS/cm
mg/L
my
ppt
Fn
m
0.50
8.27
14.33
10.49
11.83
115.10
7.6
1.00
8.35
13.23
13.64
11.65
114.20
10.4
1.50
8.29
13.2
12.18
11.93
116.20
9.2
2.00
8.21
12.89
12.57
11.91
118.40
9.6
2.50
8.14
12.97
12.93 1
12.01
1 120.80
9.9
3.00
8.13
13.06
12.98
10.14
1 125.00
9.9
0
m
0.50
8.19
13.44
10.13
11.00
96.70
7.5
1.00
8.14
13.20
10.80
11.35
98.40
8.1
1.50
8.14
12.98
11.92
11.47
100.40
9.1
2.00
8.07
12.96
12.49
11.85
101.30
9.5
2.50
8.04
13.30
12.79
12.11
102.50
9.7
3.00
8.06
13.05
12.83
12.16
103.00
9.8
O
m
0.50
8.11
12.62
10.87
10.80
147.60
8.3
1.00
8.14
12.67
12.17
10.66
149.30
9.3
1.50
8.07
12.65
12.43
10.60
149.40
9.6
2.00
8.03
12.69
12.66
12.67
149.20
9.7
2.50
8.01
12.71
12.70
10.64
149.00
9.8
3.00
8.00
12.72
12.84
12.65
148.60
9.9
`calculation

TABLE 3
MACROINVERTEBRATE TAXINOMIC IDENTIFICATION
Feeding
Group
Bio-2W
Bio-2
Bio-2E
Bio-12
Bio-13
Bio-16
Grid Count of 16
16
8"
-
Total Count
85
40
39
103
116
9
Total Taxa
7
4
7
7
9
8
43 24 24 7 2
Ameroculodes edwardsi
sdf/ssdf
16
10
5
4
0
Apocorophium simile
sdf
0
0
0
2
1
Gammarus palustris
sdf
0
0
0
1
1
(3.
Parahaustorius holmesi
ssdf
27
14
19
0
0
1 `
B1v9
Macoma.spp
31 14 11 79 91 90
ff 29 14 11 79 90 90
Rangia.spp
ff
2
0
0
0
1
0
Gastropoda 0 0 1 0 2 0
Hydrobiidae.spp
car
0
0
�1 �sj
0 ��
2
0
1vi
Polychaeta 1_1 2 3 17 21 7
Eteone heteropoda
car
2
0
1
4
6
2
Heteromastus.spp
ssdf
3
0
0
3
2
2
Nereis.spp
sdf
0
0
1
0
1
0
Spiophanes bombyx
ff/sdf
6
2
1
10
12
3
car carnivore
ff filter feeder
sdf surface deposit feeder
ssdf subsurface deposit feeder

TABLE 4
SPECIES RICHNESS AND DIVERSITY
Pestling
Group
Bia-2W Bio-2
Bio-2E
Total
Pi Pi'(LN(P);
Be 12
Bio-13
Bio-16 Total Pi Pi(LN(Pi))
Grttl Count of 16
Total Count
7 ' ---4' `__--
164
--•_+-
6
103
]
- .:;
9
Total Tava
Amplupoda
Amereculotles edwaNsi
sdgssdf
16
10
5
31
0.189
-0.315
4
1
1
1 5
0.016
-0.065
Apecorophium simile
I of
2
1
1 3
1 aixtS
-0.044
Gammarus palusfris
stlf
1
1
2
0.W6
4).032
Parahaustorius holmesi
ssdF
2]
i4
19
60
0.366
-0368
1
1
0.003
-0.018
Bivalvia
&4 c1 m spp
!!
29
14
'. 11
54
0.329
-0366
79
90
W
259
0.814
-0.167
Ran"" spp
_
ff
2
2
0012
-0.054
1
1
0.003
-0.018
Gashopoda
Hydrobudae spp oar i 1 0006 -0.031
2 2 0006 4) 032
Pol chaste
Eteone hetercpoda
car 2 _ - '-+"' 3
0.018
-0073
4
6
2 12
0.038
-0.124
Heferomastusspp
Nerns. spp
Spiophanes booebyx
sstlf —_- _=� 3
stlf 1
tlls& 9
0118
0006
1 0.055
41073
-0.Wl
-0.159
3
10
2
1
12
2 7
1
3 25
0.022
0Im
OMil
-0.084
I A0io
-0.9W
Species Richness 9 Species Richness 11
Shannonlndez 1 470 Shannonlndez 0.802
Evenness 0.669 Evenness 0334
LN

FIGURE i
NN
5
May 2014 BenMc
Bedmem Location
0 May 2015 B-N.
BeSmeni L-6-
Oecemper 2015
BenNlc True
Bam2rne L-0-
SITE LOCATION MAP
PCS PHOSPHATE COMPANY, INC., AURORA, NORTH CAROLINA

Lo
�IJ
SED SG-3
(<0.013) 810-2W -
• (<0.011 SED-DG-2
(<0.01 E1
16%
a
BACKGROUND

FIGURE 3
N
WE
S
see. r-100
:0
0 16
t May 2014 Bent0ic
O Sediment Semple
Location
May 2015 0E' BentM1ic
. ® Sediment Semple
e Location
December 2015 Benmm
SED l ® Tissue Sample Location
-- _ SW-2 BENTHIC CADMIUM (mg/Kg) ANALYSIS AN�ame�n&AA .. ea, lnc
M
_.
PCS PHOSPHATE COMPANY, INC., AURORA, NORTH CAROLINA

APPENDIX A
BENTHIC SEDIMENT SIEVE ANALYSIS

ARDAMAN & ASSOCIATES, INC. GEOTECHNICAL TESTING LABORATORY YT
WATER CONTENT DATA SHEET
ASTM STANDARD D 2216
FILE NO.: /D
TECHNICIAN:
DATE SET-UP:
DRYING TIME: X>16 hours; 0 Other:
PROJECT: SCGlewre. -1' S-,cro IeS
OVEN: ❑ 400C; 110aC; 0 Other: aC
ENGINEER: H fIWA 0�-A
hours CHECKED BY: (AS a
Other Tests
j 'SA ❑ -200
❑ ATT El -140
SA ❑ -200
❑ ATT ❑ -140
A ❑ -200
❑ ATT O -140
PrSA ❑ -200
❑ ATT 0 -140
JatA ❑ -200
❑ ATT 0 -140
Boring
Sample No.
•p
&0
ZC
t;P
12.
&
!3
Depth: ❑ Feet ❑ Meters
Material Excluded
❑ Yes
❑ Yes _
❑ Yes _
❑ Yes
❑ Yes
Sample Description ..
u,`^ ( '-f
un•l
tr.ce Si-,(((
�qrK G,qbark
wik
tts, ShAI
�^
s�n� w�
-fiacc Slcll
rk 6'1--e
.Se,...l
frwL 11
trR« 5k//
Tare No.
IJL ti I '
5 H I
563$
11S,3cf2
46`(
WVVS+Tare (9)
2-22,72✓
7✓Z'
Q✓
?-1g J78 4433,6`1
WDS+Tare (g)
162, 5OJ
197,6/
S, 1 1
1-7 2
Wt. of Tare (g)
11 7 1
111 .7 2- 11)1,771
✓
, 71 ✓
7 Z-- ✓
WDS(g)
150,?71
135, S9
MI,1y A
IQ,L
6 , 0
MC(%)
39•ci t
.32"?.I A
38�% J
I 3 5,G
34, 1 ✓
Other Tests
SA ❑ -200 1
O ATT O -140
❑ SA ❑ -200
❑ ATT O -140
❑ SA ❑ -200 ❑ SA ❑ -200
❑ ATT 0 -140 ❑ ATT O -140
❑ SA 0 -200
❑ ATT O -140
Boring
Sample No.
&;J
Depth: ❑ Feet ❑ Meters
Material Excluded
❑ Yes
❑ Yes _
❑ Yes _ ❑ Yes
❑ Yes —
Sample Description
,k%..
rd
Rrt r. 5�
Tare No.
Ns 5 r,
WWS+Tare (g)
Z(�/,
WDS+Tare (g)
192,3 I ✓
Wt. of Tare (g)
111,71 ,/
WDS (g)
/ 6 , 5 6 ✓
MC (a/o)
,3 G/ , d ,/
u see oacK or roan tor aaomcnai cara
A0128.113I1) 0v SWLS-aEOT'EC�"Laa WNUu PMM wm,m e411 oocx

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File No 113-10-10-0133E Project
Technician: JS
Date Set -UP: 12/18/2015
Front:
SEDEMENT SAMPLES
Tare No.
JS414
JS641
JS638
JS399
JS464
WWS+Tare(g)
222.72
199.37
207.88
219.78
233.64
WDS+Tare(g)
162.S8
147.61
153.05
165.15
174.82
Wt. Tare (g)
11.71
11.72
11.71
11.71
11.72
WDS (g)
150.87
/135.89
141.34
153.4
3.10
MC (%)
39.9
38.1
38.8
35.6
36.1
Tare No.
JS556
0
0
0
0
WWS+Tare(g)
261.77
0.00
0.00
0.00
0.00
WDS+Tare (g)
198.33
0.00
0.00
0.00
0.00
Wt. Tare (g)
11.77
0.00
0.00
0.00
0.00
WDS(g)
186.56
0.00
0.00
0.00
0.00
MC (%)
34.0
#DIV/01
#DIV/01
#DIV/01
#DIV/01
Back:
Tare No.
0
0
0
0
D
WWS+Tare (g)
0.00
0.00
0.00
0.00
0.00
WDS+Tare (g)
0.00
0.00
0.00
0.00
0.00
Wt. Tare (g)
0.00
0.00
0.00
0.00
0.00
WDS (g)
0.00
0.00
0.00
0.00
0.00
MC (%)
#DIV/0!
#DIV/0!
#DIV/01
#DIV/01
#DIV/011
Tare No.
0
0
0
0
0
WWS+Tare(g)
0.00
0.00
0.00
0.00
0.00
WDS+Tare (g)
0.00
0.00
0.00
0.00
0.00
Wt. Tare (g)
0.00
0.00
0.00
0.00
0.00
WDS (g)
0.00
0.00
0.00
0.00
0.00
MC (%)
#DIV/01
#DIV/01
#DIV/01
#DIV/0!
#DIV/01
109
I/ l

APPENDIX B
BENTHIC MACROINVERTEBRATE IDENTIFICATION

SAMPLEID Q)�O 9W LOCATION Para);ro ever- PROJECT -7�
Date Collected 9 ',�>e� is Grids: sortedhotal I u, t Mult. Factor-2LL—SorterZw Date-Jr� ao t ►.
QC? Y or N by Total_TaxaOther
A ti:
OcDa.-
Pq IC4 a2
I
r' Czv�
aco w /'
Ol G T
r 3'
i
Comments:
x = not counted as a separate taxon

SAMPLE ID 310 aF- LOCATION P+. )40 rZ: �. PROJECT 13
Date Collected9 VLeL- l'�- Grids: sorteditotal t e f tb Mult. Factor 1 Sorter -rvvA- Date 3 PJA 16
0r�? Y or N by Totala�—Taxa ` Other info Log# :873 9000a,
Dn VC O_G
P rc � r` rtt c.o
of
,
aS
,Ji V41V t
Mimeo.-.c, G
k�2o 44
iVe I
%;ommenta:
x not counted as a se rate taxon

SAMPLE ID %I O A LOCATION ?arn I t r v R; vc � PROJECT 7�—
Date Collected 9 t�c IS Grids: sortedhotal t" 1 In MuIL Foctor_ _Sorter v Date 3 Feb 14
QC? Y or N by Total—qo Taxa--LL_Other

SAMPLE ID 13) (a t3 LOCATION Pt: - c- PROJECT 7�_
Date Collected l0 t -c, is Grids: sorted/total q /I Io Mult. Factor /,S Sorter Date j.�.
°? Y or N by Total /�Taxa--a_Other info Log# 6731 o0o j
A m9aa
r qm r� Ivy
oro !
1n.
r l
S t rc.
C �
tv I,lr
70
0.n t
Comments:
x = not counted as a -separate taxon

SAMPLE ID DLO 1(o LOCATION Phm)�ca q:vti PRGJECT 73J
Date Collected Iv) pc- 1S Grids: sorteditotal ��I to Mult. Factor - -1 Sorter Tw%- Date 3-LtA3 IL
QC? Y or N by Total--q—q_Taxa (o Other info Log#ajLUma t
M
x = not counted as a separate taxon

SAMPLE ID l3(f) 12 LOCATION f�mDro A.' cr- PROJECT 731
Date Collected 1Q Inc_ 1 f Grids: sortedltotal �°/ i Mult. Factor -21 Sorter r11 Date yF,6 /6
^' 9 Y or N by Total /03 TaxaOther info Log# r3? 3 9 000 6

APPENDIX C
BENTHIC MACROINVERTEBRATE CADMIUM TISSUE ANALYSIS

BAL Report 1551023
ROOKS PPLI ED LABS
18804 North Creek Parkway, Ste 100, Bothell, WA 98011 • USA • T: 206 632 6206 F: 206 632 6017 • [e-mail]
January 28, 2016
Ardaman & Associates, Inc.
ATTN: David P. Scarboro
8008 S. Orange Ave.
Orlando FL 32809
[e-mail]
RE: Project ARD-FL1501 Client Project: Benthic Macroinvertebrate, Pamlico River
Dear Mr. Scarboro,
On December 16, 2016, Brooks Applied Labs (BAL) received six (6) samples. The samples were logged -
in for the analyses of total cadmium (Cd) according to the chain -of -custody form. All samples were
received, prepared, analyzed, and stored according to BAL SOPs and EPA methodology.
Trace metals were analyzed using inductively coupled plasma triple quadrupole mass' spectrometry (ICP-
QQQ-MS). The ICP-QQQ-MS uses advanced interference removal techniques to ensure accuracy of the
sample results. For more information, please visit the Interference Reduction Technology section on our
website, brooksagglied.com.
The results were method blank corrected as described in the calculations section of the relevant BAL
SOP(s) and may have been evaluated using reporting limits that have been adjusted to account for
sample aliquot size. Please refer to the Sample Results page for sample -specific MDLs, MRLs, and other
details.
Due to limited sample volume a matrix spike and matrix spike duplicate could not be performed on one
native sample. A separate method duplicate and matrix spike were prepared and reported.
The method duplicate performed on sample Bio 13 (1551023-05) produced an elevated relative percent
difference of 39%. The cause was likely sample heterogeneity. All samples were homogenized at BAL
and fragments of the may have been completely pureed. All data was reported without further
qualification, aside from concentration qualifiers, and all associated quality control sample results met
the acceptance criteria.
BAL, an accredited laboratory, certifies that the reported results of all analyses for which BAL is NELAP
accredited meet all NELAP requirements. For more information please see the Report Information page
in your report. Please feel free to contact me if you have any questions regarding this report.
Sincerely,
8PA"_
Tiffany tilwater
Client Services Manager
[e-mail]
www.brooksapplied.com
1 of 10

Project ID: ARD-FL1501
PM: Tiffany Stilwater
BROOKS
APPLIED
LABS
Report Information
BAL Report 1551023
Client PM: David Scarboro
Client PO: 10.10.133E
Laboratory Accreditation
BAL is accredited by the National Environmental Laboratory Accreditation Program (NELAP) through the State of Florida
Department of Health, Bureau of Laboratories (E87982) and is certified to perform many environmental analyses. BAL is
also certified by many other states to perform environmental analyses. For a current list of our
accreditations/certifications, please visit our website at <http://www.brooksapplied.com/resources/certificates-permits/>.
Results reported relate only to the samples listed in the report.
Field Quality Control Samples
Please be notified that certain EPA methods require the collection of field quality control samples of an appropriate type
and frequency, failure to do so is considered a deviation from some methods and for compliance purposes should only be
done with the approval of regulatory authorities. Please see the specific EPA methods for details regarding required field
quality control samples.
Common Abbreviations
BLK
method blank
MS
matrix spike
BAL
Brooks Applied Labs
MSD
matrix spike duplicate
BS
laboratory fortified blank
ND
non -detect
CAL
calibration standard
NR
non -reportable
CCB
continuing calibration blank
N/C
not calculated
CCV
continuing calibration verification
PS
post preparation spike
COC
chain of custody record
REC
percent recovery
D
dissolved fraction
RPD
relative percent difference
DUP
duplicate
RSD
relative standard deviation
IBL
instrument blank
SCV
secondary calibration verification
ICV
initial calibration verification
SOP
standard operating procedure
MDL
method detection limit
SRM
standard reference material
MRL
method reporting limit
T
total recoverable fraction
Definition of Data Qualifiers
(Effective 9123109)
B Detected by the instrument, the result is > the MDL but <_ the MRL. Result is reported and considered an estimate.
E An estimated value due to the presence of interferences. A full explanation is presented in the narrative.
H Holding time and/or preservation requirements not met. Result is estimated.
J Estimated value. A full explanation is presented in the narrative.
J-M Duplicate precision (RPD) for associated QC sample was not within acceptance criteria. Result is estimated.
J-N Spike recovery for associated QC sample was not within acceptance criteria. Result is estimated.
M Duplicate precision (RPD) was not within acceptance criteria. Result is estimated.
N Spike recovery was not within acceptance criteria. Result is estimated.
R Rejected, unusable value. A full explanation is presented in the narrative.
U Result is 5 the MDL or client requested reporting limit (CRRL). Result reported as the MDL or CRRL.
X Result is not BLK-corrected and is within 1 Ox the absolute value of the highest detectable BLK in the batch.
Result is estimated.
These qualifiers are based on those previously utilized by Brooks Applied Labs, those found in the EPA SOW ILM03.0,
Exhibit B, Section III, pg. B-18, and the USEPA Contract Laboratory Program National Functional Guidelines for Inorganic
Superfund Data Review: USEPA: January 2010. These supersede all previous qualifiers ever employed by BAL.
r
18804 North Creek Parkway, Suite 100, Bothell. WA 98011 P(206) 632-6206 F(206) 632-6017 [e-mail] www.brooksapplied.com
2 of 10

Project ID: ARD-FL1501
PM: Tiffany Stilwater
BRooKS
APPLIED
LABS
Sample Information
SAL Report 1551023
Client PM: David Scarboro
Client PO: 10.10.133E
Sample
Lab ID
Report Matrix
Type
Sampled
Received
Bio 2E
1551023-01
Macro -Inverts
Sample
unknown
12/16/2015
Bio 2
1551023-02
Macro -Inverts
Sample
unknown
12/16/2015
Bio 2W
1551023-03
Macro -Inverts
Sample
unknown
12116/2015
Bio 16
1551023-04
Macro -Inverts
Sample
unknown
12/16/2015
Bio 13
1551023-05
Macro -Inverts
Sample
unknown
12/16/2015
Bio 12
1551023-06
Macro -Inverts
Sample
unknown
12116/2015
Batch
Summary
Analyte
Lab Matrix Method
Prepared
Analyzed
Batch
Sequence
Cd
Biota EPA 1638Mod
01/18/2016
01/22/2016
B152369
1600085
18804 North Creek Parkway, Suite 100. Bothell. WA 98011 - P(206) 632-6206 F(206) 632-6017 [e-mail] www.brooksapplied.corn
3 of 10

BAL Report 1551023
Project ID: ARD-FL1501 Client PM: David Scarboro
PM: Tiffany Stilwater BRooKS Client PO: 10.10.133E
APPLIED
IJ�BS
Sample Results
Sample Analyte Report Matrix Basis Result Qualifier MDL MRL Unit Batch Sequence
Bio 12
1551023-06
Cd
Macro -Inverts
wet
0.402
0.001
0.003
mg/kg
B152369
1600085
Bio 13
1551023-05
Cd
Macro -Inverts
wet
0,347
M 0.001
0.002
mg/kg
B152369
1600085
Bio 16
1551023-04
Cd
Macro -Inverts
wet
0.320
0.001
0.003
mg/kg
B152369
1600085
Bio 2
1551023-02
Cd
Macro -Inverts
wet
0.023
0.002
0.005
mg/kg
B152369
1600085
Bio 2E
1551023-01
Cd
Macro -Inverts
wet
0.008
B 0.005
0.010
mg/kg
B152369
1600085
Bio 2W
1551023-03
Cd
Macro -Inverts
wet
0.010
0.003
0.005
mg/kg
B152369
1600085
18804 North Creek Parkway, Suite 100. Bothell, WA 98011 P(206) 632-6206 - F(206) 632-6017 - [e-mail] www.brooksapplied.com
4 of 10

Project ID: ARD-FL1501 BROOKS
PM: Tiffany Stilwater
APPLIED
LABS
Accuracy & Precision Summary
Batch:B152369
Lab Matrix: Biota
Method: EPA 1638 Mod
Sample Analyte Native Spike
B162369-BSI Laboratory Fortified Blank, (1604038)
Cd 2.500
BAL Report 1551023
Client PM: David Scarboro
Client PO: 10.10.133E
Result Units REC & Limits RPD & Limits
2.491 mg/kg 100% 75-125
B152369-SRM1 Certified Reference Material, (NC00447, DORM-3 CRM std-01-14-18)
Cd 0.2900 0.324 mg/kg 112% 75-125
B152369-DUP1 Duplicate, (1651023-05)
Cd 0.347
0.233 mg/kg
B152369-MS1 Matrix Spike, (1551023-06)
Cd 0.402 4.937 5.361 mg/kg 100% 70-130
Method Blanks & Reporting Limits
Batch:B152369
Matrix: Biota
Method: EPA 1638 Mod
Analyte: Cd
Sample
Result
B152369-BLK1
-0.001
B152369-BLK2
-0.001
B152369-BLK3
-0.0004
B152369-BLK4
-0.001
Average: -0.001
Limit: 0.005
Units
mg/kg
mg/kg
mg/kg
mg/kg
Standard Deviation: 0.000
Limit: 0.003
MDL: 0.002
MRL: 0.005
39% 30
18804 North Creek Parkway. Suite 100. Bothell. WA 98011 P(206) 632-6206 F(206) 632-6017 - info@brooksappliedcom www.brooksapplied.com
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BAL Report 1551023
Project ID: ARD-FL1501 Client PM: David Scarboro
PM: Tiffany Stilwater BROOKS Client PO: 10.10.133E
APPLIED
LABS
Sample Containers
Lab ID: 1551023-01
Report Matrix: Macro -Inverts
Collected: unknown
Sample: Bio 2E
Sample Type: Sample
Received: 12/16/2015
Des Container
Size
Lot Preservation
P-Lot
pH Ship. Cont.
A Client -Provided
40ml
not none
none
cooler
provided
Lab ID: 1551023-02
Report Matrix: Macro -Inverts
Collected: unknown
Sample: Bio 2
Sample Type: Sample
Received: 12/16/2015
Des Container
Size
Lot Preservation
P-Lot
pH Ship. Cont.
A Client -Provided
40ml
not none
none
cooler
provided
Lab ID: 1551023-03
Report Matrix: Macro -Inverts
Collected: unknown
Sample: Bio 2W
Sample Type: Sample
Received: 12/16/2015
Des Container
Size
Lot Preservation
P-Lot
pH Ship. Cont.
A Client -Provided
40mi
not none
none
cooler
provided
Lab ID: 1551023-04
Report Matrix: Macro -Inverts
Collected: unknown
Sample: Bio 16
Sample Type: Sample
Received: 12/16/2015
Des Container
Size
Lot Preservation
P-Lot
pH Ship. Cont.
A Client -Provided
40ml
not none
none
cooler
provided
Lab ID: 1551023-05
Report Matrix: Macro -Inverts
Collected: unknown
Sample: Bio 13
Sample Type: Sample
Received: 12/16/2015
Des Container
Size
Lot Preservation
P-Lot
pH Ship. Cont.
A Client -Provided
40ml
not none
none
cooler
provided
Lab ID: 1551023-06
Report Matrix: Macro -Inverts
Collected: unknown
Sample: Bio 12
Sample Type: Sample
Received: 12116/2015
Des Container
Size
Lot Preservation
P-Lot
pH Ship. Cont.
A Client -Provided
40ml
not none
none
cooler
provided
r
18804 North Creek Parkway. Suite 100. Bothell. WA 98011 P(206) 632-6206 F(206) 632-6017 - [e-mail] www.brooksapplied.com
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Project ID: ARD-FL1501
PM: Tiffany Stilwater
cooler
Received: December 16, 2015 10:35
Tracking No: 775214581750 via FedEx
Coolant Type: Dry Ice
Temperature: -32.0 °C
BROOKS
APPLIED
LABS
Shipping Containers
Description: cooler
Damaged in transit? No
Returned to client? No
BAL Report 1551023
Client PM: David Scarboro
Client PO: 10.10.133E
Custody seals present? No
Custody seals intact? No
CDC present? Yes
18804 North Creek Parkway, Suite 100. Bothell, WA 98011 P(206) 632-6206 F(206) 632-6017 [e-mail] www.brooksapplied.com
7of10

BROOKS
RAND
LABS
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MEANINGFUL MMLS DATA
3958 6� Avenue NW
Seattle, WA 98107
Phone: [phone]
Fax [phone]
[e-mail]
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Chain of Custody Record
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PO #: D. l0. l -
Phone #: Llo 7 5S 3910
Fax #: No7 9'3 V Zl
Requested TAT in
Collection
Miscellaneous
Field
Analyses
required
Comments
business days:
Preservation
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- Ytler 6. ' I

BAL Report 1551023
Ardaman & Associates, Inc.
Geotechnical, Environmental and December 15, 2015
Materials Consultants File No. 10-10-0133E
Tiffany Stilwater
Brooks Applied Labs
18804 North Creek Parkway, Suite 100
Bothell, Washington 98011
RE: Benthic Macroinvertebrate, Pamlico River
Dear Tiffany:
Enclosed are the benthic macroinvertebrate samples I collected with a Van Veen dredge in the
near shore waters of the Pamlico River on December 91 and 10th, 2015, Water quality
characteristics were taken at each of the sample sites. A table below is included that indicated
the water quality parameters taken at the sediment interface prior to collecting benthic samples.
Date
Time
Sample ID
DepthP8.00
TempConductivityDO
ORP
EDST
Ft
C
mS/cm
m A
mV
20151209
12:30
Bio 2E
2.00
12.72
8.37
12.33
189.3
20151209
13:30
Bio 2
2.0013.00
8.15
14.10
142.6
20151209
14:30
Bio 2W
2.00
12.77
8.43
14.86
152.7
20151210
11:45
Bio 16
3.00
12.72
12.84
12.65
148.6
20151210
13:30
Bio13
3.00
.
13.05
12.83
12.16
103.0
20151210
14:50
Bio 12
3.00
8.13
13.06
12.98
10.14
125.0
I am unsure if the conductivity of the river water will add any interference to the samples. Each
sample was rinsed in site water then in distilled water during the picking process. A small amount
of distilled water was added to the vials as the samples were washed from the small container
into the sample vial.
Small soft bivalves and their shells were included in the samples due in -part to their soft nature.
Hard clams that were very small were shucked and their soft tissue included in the biomass of
the sample. There may be some sands within the sample that will need to be removed before the
sample is analyzed.
Each sample was decanted to the best of my ability and placed on dry ice after the clean/ dirty
hands sampling procedure.
Please let me know if there is anything you will need for the analysis.
Best Regards,
ARDAMAN & ASSOCIATES, INC.
David Scarboro
Environmental Scientist III
B008 S. Orange Avenue 32809, Post Office Box 593003. Orlando, Florida 32859-3003 Phone [phone] FA% [phone]
Louisiana: Alexandria, Baton Rouge. Monroe, New Orleans, Shreveport
Florida: Bartow, Cocoa, Fort Myers, Miami, Orlando, 50 lSb Lucie, Sarasota, Tallahassee, Tampa, West Palm Beach

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10 of 10