NC0055786_Renewal (Application)_20131118

AFTIA
NCDENR
North Carolina Department of Environment and Natural Resources
Pat McCrory
Governor
November 18, 2013
Lexington Regional WTP
28 West Center Street
Lexington, NC 27292
Subject: Receipt of permit renewal application
NPDES Permit NCO055786
Davidson County
Dear Mr. Roger Spach,
John E. Skvarla, III
Secretary
RECEIVED
N.C. Dept. of ENR
NOV 2 1 2013
W NSTON-SALEM
REGIONAL OFFICE
The NPDES Unit received your permit renewal application on November 4th, 2013. This
permit renewal has been assigned Julie Grzyb (919-807-6389) who will contact you if any
additional information is required to complete your permit renewal. Due to current backlog, you
should continue to operate under terms of your current permit, until a new permit is issued. If
you have any questions, please contact the assigned permit writer.
Sincerely,
i 60) #1 a
Jeff Poupart
Point Source Branch Program Supervisor IV
Cc: Central Files
Winton -Salem Regional Office
NPDES Unit
1601 Mail Service Center, Raleigh, North Carolina 27699-1601 One
Phone: [phone] Internet: www.ncdenr.gov Nol'thCarolina
An Equal Opportunity1 Affirmative Action Employer— 50% Recycled 110% Post Consumer Paper Amorally

^� "o N.CRE EIVED
U x Pt of ENR
NOV 2 1 2013
ORI.16R
wlNsro
FFE IC
City of Lexington, NC REGIONAL O
Department of Water Resources
October 28, 2013
Mr. Charles Weaver
NCDENR/DWR/Point Source Branch
1617 Mail Service Center
Raleigh, NC 27699-1617
Subject: NPDES Permit — NCO055786
Lexington Regional WWTP
Davidson County
Application for Permit Renewal
Dear Mr. Weaver:
By this letter and attachments, I am requesting renewal of the NPDES permit for the City
of Lexington's Regional WWTP — NC0055786.
Attached are the following: EPA Form 2A — with attachments
Sludge Management Plan: The City disposes of the sludge generated at this facility
through its Compost Facility — WQ0001318.
Please forward comments and questions to my attention at 336-248-3970 or to
RASpach a-Jexingtonnc.gov.
Sincerely
4�-�Z�
Roger pach,
Water Resources Superintendent
CC: Rick Comer
Joe Shaffer
Jennifer Carter

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 I renewal Yadkin Pee -Dee
All treatment works must complete questions A.1 through A.8 of this Basic Application Information Packet.
A.I. Facility Information.
Facility Name Lexington Regional Wastewater Treatment Plant
Mailing Address City of Lexington
28 West Center Street, Lexington, NC 27292
Contact Person
Title Water Resources Superintendent
Telephone Number [phone]
Facility Address 500 Glendale Road, Lexington, NC 27292
(not P.O. Box)
A.2. Applicant Information. If the applicant is different from the above, provide the following:
Applicant Name
Mailing Address
Contact Person
IIIHM
Telephone Number ( 1
Is the applicant the owner or operator (or both) of the treatment works?
❑ owner ® operator
Indicate whether correspondence regarding this permit should be directed to the facility or the applicant.
❑ facility ® applicant
A.3. Existing Environmental Permits. Provide the permit number of any existing environmental permits that have been issued to the treatment works
(include state -issued permits).
NPDES NCO055786 PSD
UIC Other WQ0001318: WQ0023213: WQ0016165
RCRA Other NCG110093
A.4. Collection System Information. Provide information on municipalities and areas served by the facility. Provide the name and population of each
entity and, if known, provide information on the type of collection system (combined vs. separate) and its ownership (municipal, private, etc.).
Name Population Served Type of Collection System Ownership
City of Lexington +/- 30,000 Separate Municipal
Total population served +/- 30.000
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 2 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
A.S. Indian Country.
a. Is the treatment works located in Indian Country?
❑ Yes (0 No
b. Does the treatment works discharge to a receiving water that is either in Indian Country or that is upstream from (and eventually flows
through) Indian Country?
❑ Yes ® No (to the best of my knowledge)
A.6. Flow. Indicate the design flow rate of the treatment plant (i.e., the wastewater flow rate that the plant was built to handle). Also provide the
average daily flow rate and maximum daily flow rate for each of the last three years. Each year's data must be based on a 12-month time period
with the 12`h month of "this year" occurring no more than three months prior to this application submittal.
a. Design flow rate 6.5 MGD
Two Years Ago Last Year
b. Annual average daily flow rate 2.98 2.80 2.75
This Year
C. Maximum daily flow rate 8.47 7.59 6.40
A.7. Collection System. Indicate the type(s) of collection system(s) used by the treatment plant. Check all that apply. Also estimate the percent
contribution (by miles) of each.
® Separate sanitary sewer 100 %
❑ Combined storm and sanitary sewer %
A.8. Discharges and Other Disposal Methods.
a. Does the treatment works discharge effluent to waters of the U.S.? ® Yes ❑ No
If yes, list how many of each of the following types of discharge points the treatment works uses:
i. Discharges of treated effluent
ii. Discharges of untreated or partially treated effluent
III. Combined sewer overflow points
iv. Constructed emergency overflows (prior to the headworks)
V. Other
b. Does the treatment works discharge effluent to basins, ponds, or other surface impoundments
that do not have outlets for discharge to waters of the U.S.? ❑ Yes
If yes, provide the following for each surface impoundment:
Location:
Annual average daily volume discharge to surface impoundment(s)
Is discharge ❑ continuous or ❑ intermittent?
C. Does the treatment works land -apply treated wastewater?
If yes, provide the following for each land application site:
Location: on site (W00016165)
Number of acres: 3.88
1
® No
MGD
® Yes ❑ No
Annual average daily volume applied to site: .008 MGD
Is land application ❑ continuous or ® intermittent?
d. Does the treatment works discharge or transport treated or untreated wastewater to another
treatment works? ® Yes
❑ No
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 3 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Lexington Regional WWTP, NCO055786
renewal
Yadkin Pee-dee
If yes, describe the mean(s) by which the wastewater from the treatment works is discharged or transported to the other treatment works
(e.g., tank truck, pipe).
Lexington operates a conjunctive use plant — W00023213 that intercepts wastewater from an outfall leading to the Regional Plant This
water is treated to reuse standards with a package plant and is used to irrigate the Lexington Golf Course
If transport is by a party other than the applicant, provide:
Transporter Name
Mailing Address
Contact Person
Title
Telephone Number ) )
For each treatment works that receives this discharge, provide the following:
Name Lexington Golf Course Coniunctive Wastewater Treatment and Reclaimed Water Utilization System— WQ0023213
Mailing Address City of Lexington, 28 West Center Street, Lexington, NC 27292
Contact Person Roger Spach
Title Superintendent
Telephone Number (336 ) 248-3970
If known, provide the NPDES permit number of the treatment works that receives this discharge NA
Provide the average daily flow rate from the treatment works into the receiving facility. <0.3 MGD
e. Does the treatment works discharge or dispose of its wastewater in a manner not included
in A.8. through A.8.d above (e.g., underground percolation, well injection): ® Yes ❑ No
If yes, provide the following for each disposal method:
Description of method (including location and size of site(s) if applicable):
Spray irrigation — approximately 87 acres
Annual daily volume disposed by this method: Weather dependent
Is disposal through this method ❑ continuous or ® intermittent?
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 4 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
WASTEWATER DISCHARGES:
If you answered "Yes" to question A.8.a, complete questions A.9 through A.12 once for each outfall (including bypass points) through
which effluent is discharged. Do not include information on combined sewer overflows in this section. If you answered "No" to question
A.8.a, go to Part B, "Additional Application Information for Applicants with a Design Flow Greater than or Equal to 0.1 MGD."
A.9. Description of Outfall.
a. Outfall number 001
b. Location Lexington 27292
(City or town, if applicable) (Zip Code)
(County)
(State)
35°46'06"
80'14'14"
(Latitude)
(Longitude)
C. Distance from shore (if applicable)
ft.
d. Depth below surface (if applicable)
ft.
e. Average daily flow rate 2.8
MGD
f. Does this ouffall have either an intermittent or a periodic discharge? ❑ Yes
® No (go to A.9.g.)
If yes, provide the following information:
Number f times per year discharge occurs:
Average duration of each discharge:
Average flow per discharge:
MGD
Months in which discharge occurs:
g. Is ouffall equipped with a diffuser? ❑ Yes
® No
A.10. Description of Receiving Waters.
a. Name of receiving water Abbotts Creek
b. Name of watershed (if known)
United States Soil Conservation Service 14-digit watershed code (if known):
C. Name of State Management/River Basin (if known):
United States Geological Survey 8-digit hydrologic cataloging unit code (if known):
d. Critical low flow of receiving stream (if applicable)
acute cfs chronic cfs
e. Total hardness of receiving stream at critical low flow (if applicable): mg/I of CaCO3
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 $ 7550-22. Page 6 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP. NCO055786 renewal I Yadkin Pee -Dee
A.11. Description of Treatment
a. What laud of treatment are provided? Check all that apply.
® Primary ® Secondary
® Advanced ❑ Other. Describe: _
b. Indicate the following removal rates (as applicable):
Design BOD5 removal or Design CBOD5 removal
Design SS removal
Design P removal
Design N removal
Other
c. What type of disinfection is used for the effluent from this outfall? If disinfection varies by season, please describe:
If disinfection is by chlorination is dechlorination used for this outfall? ® Yea
Does the treatment plant have post aeration? ® Yes
98(influent design-271 effluent limit 51 %
82 (influent design -169 effluent limit 30) %
63(influent design-6 effluent limd of l l %
na %
❑ No
❑ No
A.12. Effluent Testing Information. All Applicants that discharge to waters of the US must provide effluent testing data for the following parameters. Provide the Indicated effluent testing required by the
p—Ming authority for each outfall through which effluent Is discharoed. Do not Include Information on combined sewer overflows in this section. All informatlon reported must be based on data collected
through anaryels conducted using 40 CFR Part 136 methods. In addtilon, this data must comply with QAIQC requirements of 40 CFR Part 136 and other appropriate QAIQC requirements for standard
methods for analytes not addressed by 40 CFR Part 136. At a minimum, effluent testing data must be based on at least three samples and must be no more then four and ores -half years apart.
Outfall number: 001 (August 2012—JuIv2013 data)
pH (Minimum)
6.0
S.U.
pH (Maximum)
8.6
s.U.
Flow Rate
8.47
mgd
2.84
m d
1095
Temperature (Winter)
19
DC
14
°C
1095
Temperature (Summer)
30
°C
22
DC
1095
CONVENTIONAL AND NON CONVENTIONAL COMPOUNDS
BIOCHEMICAL OXYGEN BOD5 8.07 M /I 1.78 M /I 748
DEMAND (Report one) CBOD5
FECAL COLIFORM 136 /100 ml 2.3 /100 ml 748
TOTAL SUSPENDED SOLIDS (TSS) 11.9 Mail 2.93 Mel/I 748
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 6 of 22

FACILITY NAME AND PERMIT NUMBER: I PERMIT ACTION REQUESTED: I RIVER BASIN:
Lexington Regional WWTP, NCO055786
renewal
Yadkin Pee -Dee
All applicants with a design flow rate 2 0.1 MGD must answer questions B.1 through B.6. All others go to Part C (Certification).
B.1. Inflow and Infiltration. Estimate the average number of gallons per day that flow into the treatment works from inflow and/or infiltration.
500,000 gpd — Based upon a year around average
Briefly explain any steps underway or planned to minimize inflow and infiltration.
10 % of the systems sewer lines are inspected annually. Annual replacement/slip lining of older lines as can be budgeted,
TV inspection of lines. Grease trap disposal program
B.2. Topographic Map. Attach to this application a topographic map of the area extending at least one mile beyond facility property boundaries. This
map must show the outline of the facility and the following information. (You may submit more than one map if one map does not show the entire
area.)
a. The area surrounding the treatment plant, including all unit processes. Attachment 1
b. The major pipes or other structures through which wastewater enters the treatment works and the pipes or other structures through which
treated wastewater is discharged from the treatment plant. Include outfalls from bypass piping, if applicable. Attachment 2
c. Each well where wastewater from the treatment plant is injected underground. NA
d. Wells, springs, other surface water bodies, and drinking water wells that are: 1) within '/. mile of the property boundaries of the treatment
works, and 2) listed in public record or otherwise known to the applicant. See attachment 1
e. Any areas where the sewage sludge produced by the treatment works is stored, treated, or disposed. Attachment 3
f. If the treatment works receives waste that is classed as hazardous under the Resource Conservation and Recovery Act (RCRA) by truck, rail,
or special pipe, show on the map where the hazardous waste enters the treatment works and where it is treated, stored, and/or disposed. NA
B.3. Process Flow Diagram or Schematic. Provide a diagram showing the processes of the treatment plant, including all bypass piping and all
backup power sources or redunancy in the system. Also provide a water balance showing all treatment units, including disinfection (e.g.,
chlorination and dechlorination). The water balance must show daily average flow rates at influent and discharge points and approximate daily flow
rates between treatment units. Include a brief narrative description of the diagram. Attachment 4
BA. Operation/Maintenance Performed by Contractor(s).
Are any operational or maintenance aspects (related to wastewater treatment and effluent quality) of the treatment works the responsibility of a
contractor? ® Yes ❑ No
If yes, list the name, address, telephone number, and status of each contractor and describe the contractor's responsibilities (attach additional
pages if necessary).
Name: ISUnc.
Mailing Address:
Telephone Number: C )
Responsibilities of Contractor: Maintain and Calibrate plant instrumentation
B.5. Scheduled improvements and Schedules of Implementation. Provide information on any uncompleted implementation schedule or
uncompleted plans for improvements that will affect the wastewater treatment, effluent quality, or design capacity of the treatment works. If the
treatment works has several different implementation schedules or is planning several improvements, submit separate responses to question B.5
for each. (If none, go to question B.6.)
List the outfall number (assigned in question A.9) for each outfall that is covered by this implementation schedule.
b. Indicate whether the planned improvements or implementation schedule are required by local, State, or Federal agencies.
❑ Yes ® No
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 7 of 22

EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 8 of 22

FACILITY NAME AND PERMIT NUMBER: I PERMIT ACTION REQUESTED: I RIVER BASIN:
Lexington Regional WWTP, NCO055786
renewal
Yadkin Pee -Dee
All applicants must complete the Certification Section. Refer to instructions to determine who is an officer for the purposes of this
certification. All applicants must complete all applicable sections of Form 2A, as explained in the Application Overview. Indicate below which
parts of Form 2A you have completed and are submitting. By signing this certification statement, applicants confirm that they have reviewed
Form 2A and have completed all sections that apply to the facility for which this application is submitted.
Indicate which parts of Form 2A you have completed and are submitting:
® Basic Application Information packet Supplemental Application Information packet:
❑ Part D (Expanded Effluent Testing Data)
® Part E (Toxicity Testing: Biomonitoring Data)
® Part F (Industrial User Discharges and RCRA/CERCLA Wastes)
❑ Part G (Combined Sewer Systems)
I certify under penalty of law that this document and all attachments were prepared under my direction or supervision in accordance with a system
designed to assure that qualified personnel properly gather and evaluate the information submitted. Based on my inquiry of the person or persons who
manage the system or those persons directly responsible for gathering the information, the information is, to the best of my knowledge and belief, true,
accurate, and complete. I am aware that there are significant penalties for submitting false information, including the possibility of fine and imprisonment
for knowing violations.
Name and official title
Roper Sp;
t
TC3
Signature
Telephone number
336 2248-
Date signed
16
6L3
Upon request of the permitting authority, you must submit any other information necessary to assure wastewater treatment practices at the treatment
works or identify appropriate permitting requirements.
SEND COMPLETED FORMS TO:
NCDENR/ DWQ
Attn: NPDES Unit
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 9 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
Refer to the directions on the cover page to determine whether this section applies to the treatment works.
Effluent Testing: 1.0 MGD and Pretreatment Works. If the treatment works has a design flow greater than or equal to 1.0 MGD or it has (or is required
to have) a pretreatment program, or is otherwise required by the permitting authority to provide the data, then provide effluent testing data for the following
pollutants. Provide the indicated effluent testing information and any other information required by the permitting authority for each outfall through which
effluent is discharged. Do not include information on combined sewer overflows in this section. All information reported must be based on data collected
through analyses conducted using 40 CFR Part 136 methods. In addition, these data must comply with QA/QC requirements of 40 CFR Part 136 and
other appropriate QAtCC requirements for standard methods for analytes not addressed by 40 CFR Part 136. Indicate in the blank rows provided below
any data you may have on pollutants not specifically listed in this form. At a minimum, effluent testing data must be based on at least three pollutant
scans and must be no more than four and one-half years old.
Outfall number:
(Complete once for each outfall discharging effluent to waters of the United States.)
METALS (TOTAL RECOVERABLE), CYANIDE, PHENOLS, AND HARDNESS. I
ANTIMONY
ARSENIC
BERYLLIUM
CADMIUM
CHROMIUM
COPPER
LEAD
MERCURY
NICKEL
SELENIUM
SILVER
THALLIUM
ZINC
CYANIDE
TOTAL PHENOLIC
COMPOUNDS
HARDNESS (as CaCO3)
Use this space (or a separate sheet) to provide information on other metals requested by the permit writer
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7560-6 & 7550-22. Page 10 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
Outhall number: (Complete once for each outfall discharging effluent to waters of the United States.)
VOLATILE ORGANIC COMPOUNDS
ACROLEIN
I ACRYLONITRILE
I BENZENE
BROMOFORM
CARBON
TETRACHLORIDE
CHLOROBENZENE
CHLORODIBROMO-
METHANE
CHLOROETHANE
2-CHLOROETHYLVINYL
ETHER
CHLOROFORM
DICHLOROBROMO-
METHANE
1,1-DICHLOROETHAN E
1,2-DICHLOROETHAN E
TRANS-I,2-DICHLORO-
ETHYLENE
1,1-DICHLORO-
ETHYLENE
1,2-DICHLOROPROPAN E
1,3-DICHLORO-
PROPYLENE
ETHYLBENZENE
METHYL BROMIDE
METHYL CHLORIDE
METHYLENE CHLORIDE
1,1,2,2-TETRA-
CHLOROETHANE
TETRACHLORO-
ETHYLENE
TOLUENE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 11 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
Outfall number: (Complete once for each outfall discharging effluent to waters of the United States.)
1,1,1-
TRICHLOROETHANE
1,1,2-
TRICHLOROETHANE
TRICHLOROETHYLENE
VINYL CHLORIDE
Use this space (or a separate sheet) to provide information on other volatile organic compounds requested by the permit writer
ACID -EXTRACTABLE COMPOUNDS
P-CHLORO-M-CRESOL
2-CHLOROPHENOL
2,4-DICHLOROPHENOL
2,4-DIMETHYLPHENOL
4,6-DIN ITRO-O-CRESOL
2,4-DINITROPHENOL
2-NITROPHENOL
4-NITROPHENOL
PENTACHLOROPHENOL
PHENOL
2,4,6-
TRICHLOROPHENOL
Use this space (or a separate sheet) to provide information on other acid -extractable compounds requested by the permit writer
BASE -NEUTRAL COMPOUNDS
ACENAPHTHENE
ACENAPHTHYLENE
ANTHRACENE
BENZIDINE
BENZO(A)ANTHRACENE
BENZO(A)PYRENE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 12 of 22

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Lexington Regional WWTP, NCO055786
renewal
Yadkin Pee -Dee
Outfall number: (Complete once for each outfall discharging effluent to waters of the United States.)
410
rq,,� RaIR"
ARM
51
3,4 BENZO-
FLUORANTHENE
BENZO(GHI)PERYLENE
BENZO(K)
FLUORANTHENE
BIS (2-CHLOROETHOXY)
METHANE
BIS (2-CHLOROETHYL)-
ETHER
BIS (2-CHLOROISO-
PROPYL) ETHER
BIS (2-ETHYLHEXYL)
PHTHALATE
4-BROMOPHENYL
PHENYL ETHER
BUTYL BENZYL
PHTHALATE
2-CHLORO-
NAPHTHALENE
4-CHLORPHENYL
PHENYLETHER
CHRYSENE
DI-N-BUTYL PHTHALATE
DI-N-OCTYL PHTHALATE
DIBENZO(A,H)
ANTHRACENE
1,2-DICHLOROBENZENE
1,3-DICHLOROBENZENE
1,4-DICHLOROBENZENE
3,3-DICHLORO-
BENZIDINE
DIETHYL PHTHALATE
DIMETHYL PHTHALATE
2,4-DINITROTOLUENE
2,6-DINITROTOLUENE
1,2-DIPHENYL-
HYDRAZINE
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 13 of 22

EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 14 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 MGD; 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E, you need not submit it again. Rather, provide the information
requested in question EA for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
E.1. Required Tests.
Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
22❑ chronic ❑ acute information in E.2 is for fat head minnow tests only. Copies of these tests are attached.
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 1 Test number: 2 Test number: 3
a. Test information.
Test Species & test method number
Pimephales
promelas/THM
Pimephales
promelas/THP6C
Pimephales
promelas/THPW
Age at initiation of test
24 hrs
24 hrs
24 hrs
Outfall number
001
001
001
Dates sample collected
2/5 - 2/7 - 2/8/2013
517 - 5/9 - 5/10/2013
8/6 - 8/8 - 8/10/2013
Date test started
2/5/2013
5/7/2013
8/6/2013
Duration
2/5 — 2/12/2013
5/7 — 5/14/2013
8/6 — 8/13/2013
b. Give toxicity test methods followed. I
Manual title
Edition number and year of publication
Page number(s)
c. Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used.
24-Hour composite I X I X X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 15 of 22

After dechlorination X X X
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 16 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
Test number: 1 Test number: 2 Test number: 3
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected: effluent effluent effluent
f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity X X X
Acute toxicity
g. Provide the type of test performed.
Static
Static -renewal
X
X
X
Flow -through
h. Source of dilution water. If laboratory water, specify type; if receiving water, specify source.
Laboratory water
Soft synthetic freshwater
Soft synthetic freshwater
Soft synthetic freshwater
Receiving water
i. Type of dilution water. If salt water, specify "natural' or type of artificial sea salts or brine used.
Fresh water
Soft synthetic freshwater
Soft synthetic freshwater
Soft synthetic freshwater
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
15, 30, 60, 80, 100
15, 30, 60, 80, 100
15, 30, 60, 80,100
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH meets meets meets
Salinity
Temperature meets meets meets
Ammonia
Dissolved oxygen meets meets meets
I. Test Results.
Acute:
Percent survival in 100%
effluent
%
%
%
LC50
95% C.I.
%
%
%
Control percent survival
%
ova
ova
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 17 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
Chronic:
NOEC 100 % 100 % 100 %
C" %
Control percent survival 87.5 % 95 % 100 %
Other (describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available? no no no
Was reference toxicant test within
acceptable bounds?
What date was reference toxicant test
run (MM/DD/YYYY)?
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes, describe:
EA. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: / / (MM/DD/YYYY)
Summary of results: (see instructions)
,
s
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 18 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
All treatment works receiving discharges from significant industrial users or which receive RCRA,CERCLA, or other remedial wastes must
complete part F.
GENERAL INFORMATION:
F.I. Pretreatment program. Does the treatment works have, or is subject ot, an approved pretreatment program?
® Yes ❑ No
F.2. Number of Significant Industrial Users (SIUs) and Categorical Industrial Users (CIUs). Provide the number of each of the following types of
industrial users that discharge to the treatment works.
a. Number of non -categorical SIUs.
b. Number of CIUs.
1
SIGNIFICANT INDUSTRIAL USER INFORMATION:
Supply the following information for each SIU. If more than one SIU discharges to the treatment works, copy questions F.3 through F.8 and
provide the information requested for each SIU.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 19 of 22

F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name: Cardinal Container Services
Mailing Address: P.O. Box 1866
Lexington NC 27292
FA. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Cleans and conditions drums
F.S. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s):
Raw material(s): drums and IBC's
F.6. Flow Rate.
Cleans and conditions drums
a. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in
gallons per day (gpd) and whether the discharge is continuous or intermittent.
33,000 gpd (X continuous or _ intermittent)
b. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged into the collection
system in gallons per day (gpd) and whether the discharge is continuous or intermittent.
4000 gpd (x continuous or _ intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes No
b. Categorical pretreatment standards Yes No
If subject to categorical pretreatment standards, which category and subcategory?
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes X No
b. Categorical pretreatment standards X Yes No
If subject to categorical pretreatment standards, which category and subcategory? 433
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name: Matcor Metal Fabrication
Mailing Address: P.O. Box 729
Welcome,NC
F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
OEM parts for off road heavy equipment
F.S. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s):Heavy Construction Equipment Welded components and generator enclosures.
Raw materal(s): Hot and cold rolled A36
F.6. Flow Rate.
Form 3590-2A (Revptd&)ssR6Rbt MbwaatW5MidaiCP!WeUverage daily volume of process wastewater discharge into the collection sys r?0 of 22
gallons per day (gpd) and whether the discharge is continuous or intermittent.

F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages as
necessary.
Name: Diebold Southeast Manufacturing,
Mailing Address: 118 Lexington Parkway
Lexington,NC 27272
FA. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Assembles Atm's and products
F.S. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's discharge.
Principal product(s):Atm's, Safe Deposit Sections, and Under counter cabinets
Raw material(s):Prepainted metal and plastic componets
F.6. Flow Rate.
a. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons
per day (gpd) and whether the discharge is continuous or intermittent.
12,000 gpd (X continuous or _ intermittent)
b. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged into the collection
system in gallons per day (gpd) and whether the discharge is continuous or intermittent.
2500 gpd (x continuous or _ intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes X No
b. Categorical pretreatment standards X Yes No
If subject to categorical pretreatment standards, which category and subcategory? 433
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages as
necessary.
Name: Leggett & Platt, Inc. Plant #1
Mailing Address: 3040 Junior Order Home road -
Lexington, NC 27292
F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Punch shear assemble angle tubular steel into metal furniture
F.S. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's discharge.
Principal product(s): Bed frames bed rails, rollaway beds
Raw material(s): roll metals, stainless
Flow Rate.
C. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in gallons
per day (gpd) and whether the discharge is continuous or intermittent.
15,000 gpd (X continuous or _ intermittent)
d. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged into the collection
system in gallons per day (gpd) and whether the discharge is continuous or intermittent.
3000 gpd (x continuous or _ intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes X No
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 21 of 22

b. Categorical pretreatment standards X Yes No
If subject to categorical pretreatment standards, which category and subcategory? 433
F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name: Leggett & Platt, Inc. Plant
Mailing Address: 161 Proctor Lane
Lexington, NC 27292
F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
, Paint punch assemble weld and pack wood and metal Pedestal beds
F.S. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s): Pedestal beds
Raw material(s): roll metals, stainless and wood
Flow Rate.
e. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in
gallons per day (gpd) and whether the discharge is continuous or intermittent.
8,000 gpd (X continuous or _ intermittent)
f. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged into the collection
system in gallons per day (gpd) and whether the discharge is continuous or intermittent.
300 r gpd (x continuous or _ intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes X No
b. Categorical pretreatment standards X Yes No
If subject to categorical pretreatment standards, which category and subcategory? 433
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 22 of 22

F.3. Significant Industrial User Information. Provide the name and address of each SIU discharging to the treatment works. Submit additional pages
as necessary.
Name: ASCO Power Technologies
Mailing Address: P.O. Box 689
Welcome,NC 2
F.4. Industrial Processes. Describe all the industrial processes that affect or contribute to the SIU's discharge.
Manufacture of electrical transfer switches and enclosures
F.S. Principal Product(s) and Raw Material(s). Describe all of the principal processes and raw materials that affect or contribute to the SIU's
discharge.
Principal product(s): Automatic Electrical Transfer Switches and Enclosures
Raw material(s): Carbon Steel, Stainless Steel, Copper Bar, Insulated copper wires
Flow Rate.
g. Process wastewater flow rate. Indicate the average daily volume of process wastewater discharge into the collection system in
gallons per day (gpd) and whether the discharge is continuous or intermittent.
8,000 gpd (X continuous or _ intermittent)
h. Non -process wastewater flow rate. Indicate the average daily volume of non -process wastewater flow discharged into the collection
system in gallons per day (gpd) and whether the discharge is continuous or intermittent.
1000 gpd (x continuous or _ intermittent)
F.7. Pretreatment Standards. Indicate whether the SIU is subject to the following:
a. Local limits Yes X No
b. Categorical pretreatment standards X Yes No
If subject to categorical pretreatment standards, which category and subcategory? 433
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 R 7550-22. Page 23 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
F.8. Problems at the Treatment Works Attributed to Waste Discharge by the SIU. Has the SIU caused or contributed to any problems (e.g.,
upsets, interference) at the treatment works in the past three years?
❑ Yes ® No If yes, describe each episode.
RCRA HAZARDOUS WASTE RECEIVED BY TRUCK, RAIL, OR DEDICATED PIPELINE:
F.9. RCRA Waste. Does the treatment works receive or has it in the past three years received RCRA hazardous waste by truck, rail or dedicated pipe?
❑ Yes ® No (go to F.12)
F.10. Waste transport. Method by which RCRA waste is received (check all that apply):
❑ Truck ❑ Rail ❑ Dedicated Pipe
F.11. Waste Description. Give EPA hazardous waste number and amount (volume or mass, specify units).
EPA Hazardous Waste Number Amount Units
CERCLA (SUPERFUND) WASTEWATER, RCRA REMEDIATION/CORRECTIVE ACTION
WASTEWATER, AND OTHER REMEDIAL ACTIVITY WASTEWATER:
F.12. Remediation Waste. Does the treatment works currently (or has it been notified that it will) receive waste from remedial activities?
❑ Yes (complete F.13 through F.15.) ❑ No
F.13. Waste Origin. Describe the site and type of facility at which the CERCLA/RCRA/or other remedial waste originates (or is excepted to origniate in
the next five years).
F.14. Pollutants. List the hazardous constituents that are received (or are expected to be received). Include data on volume and concentration, if
known. (Attach additional sheets if necessary.)
F.15. Waste Treatment.
a. Is this waste treated (or will be treated) prior to entering the treatment works?
❑ Yes ❑ No
If yes, describe the treatment (provide information about the removal efficiency):
b. Is the discharge (or will the discharge be) continuous or intermittent?
❑ Continuous ❑ Intermittent If intermittent, describe discharge schedule.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 24 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO0557896 renewal Yadkin Pee -Dee
If the treatment works has a combined sewer system, complete Part G.
G.1. System Map. Provide a map indicating the following: (may be included with Basic Application Information)
a. All CSO discharge points.
b. Sensitive use areas potentially affected by CSOs (e.g., beaches, drinking water supplies, shellfish beds, sensitive aquatic ecosystems, and
outstanding natural resource waters).
C. Waters that support threatened and endangered species potentially affected by CSOs.
G.2. System Diagram. Provide a diagram, either in the map provided in G.1 or on a separate drawing, of the combined sewer collection system that
includes the following information.
a. Location of major sewer trunk lines, both combined and separate sanitary.
b. Locations of points where separate sanitary sewers feed into the combined sewer system.
C. Locations of in -line and off-line storage structures.
d. Locations of flow -regulating devices.
e. Locations of pump stations.
CSO OUTFALLS:
Complete questions G.3 through G.6 once for each CSO discharge point.
G.3. Description of Outfall.
a. Outfall number
b. Location
(City or town, if applicable)
(Zip Code)
(County) (State)
(Latitude) (Longitude)
C. Distance from shore (if applicable) ft.
d. Depth below surface (if applicable) ft.
e. Which of the following were monitored during the last year for this CSO?
❑ Rainfall ❑ CSO pollutant concentrations
❑ CSO flow volume ❑ Receiving water quality
f. How many storm events were monitored during the last year?
GA. CSO Events.
a. Give the number of CSO events in the last year.
events (❑ actual or ❑ approx.)
b. Give the average duration per CSO event.
hours (❑ actual or ❑ approx.)
❑ CSO frequency
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 25 of 22

FACILITY NAME AND PERMIT NUMBER: PERMIT ACTION REQUESTED: RIVER BASIN:
Lexington Regional WWTP, NCO055786 renewal Yadkin Pee -Dee
C. Give the average volume per CSO event.
million gallons (❑ actual or ❑ approx.)
d. Give the minimum rainfall that caused a CSO event in the last year
Inches of rainfall
G.5. Description of Receiving Waters.
a. Name of receiving water:
b. Name of watershed/river/stream system:
United State Soil Conservation Service 14-digit watershed code (if known):
C. Name of State Management/River Basin:
United States Geological Survey 8-digit hydrologic cataloging unit code (if known):
G.6. CSO Operations.
Describe any known water quality impacts on the receiving water caused by this CSO (e.g., permanent or intermittent beach closings, permanent or
intermittent shell fish bed closings, fish kills, fish advisories, other recreational loss, or violation of any applicable State water quality standard).
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 26 of 22

Additbnal information, if provided, will appear on the following pages.
NPDES FORM 2A Additional Information

NC0055786
ATTACHMENT 1

Quad: Lexington East, N.C. N C 0 0 5 5 7 8 6 Facility
Latitude: 35°46'06"
Longitude:80°14'14" Lexington Regional Location
Stream Class: WS-V & B WWTP
Subbasin: 30707
Receiving Stream: Abbotts Creek North. SCALE 1:2�i000

F r
••
� r
�, .r
P
'RT
I

NCOOSS786
ATTACHMENT 2

Headworks r�.4
2.8 MGD `
ik'BN
2' n d F�
Wastewater
Flow
�� `���%t �. � �'• �,� sir ' .
T
C r
generator
4
R
Chlorination
stage
"'� Q,
lift rT
• .
..
rd stationt��.`,
�.,y .t
Final :{9
Clarifiers
Polishing
lagoons
dechlorination
Post
aeration
r r71
Effluent
2.8 M G D

NCO055786
ATTACHMENT 3

Vk
x

NCO055786
ATTACHMENT 4

Permit#NC0055786
Section B.3. Narrative:
Wastewater Treatment Process
Influent - An average of 2.8 MGD enters the Lexington Regional Wastewater Treatment Plant through a
single outfall that parallels Abbotts Creek.
Preliminary Treatment - Wastewater first goes through a bar screen, then it goes through the plant's
first stage lift station. The lift station consists of 4 submersible pumps and one screw pump. The lift
station presently discharges into a grit separator. A coarse bar screen was initially between the pump
discharge and the grit separator. This was removed when the plant was upgraded in 2005. Within the
next 12 months, plant staff will install a fine screen where the old coarse screen was. After the
wastewater exits the grit separator, it flows through a parshall flume and then flows by gravity to a
second stage lift station which is identical to the first stage.
BNR — Following the second stage lift station, wastewater flows by gravity to the plants BNR system,
This system is presently operated for phosphorous removal only. Nitrogen removal is incidental. Alum
is added as needed to enhance phosphorous removal. The BNR system consists to two identical units
with the influent flow split equally between them.
Final Clarifiers — Wastewater exiting the two final clarifiers is split between two final clarifiers. Return
activated sludge is recycled through the BNR system and a portion of it is wasted into a DAF unit. The
overflow from the DAF unit is returned to the head of the second stage lift station
Polishine - Following the clarifiers, the settled wastewater flows by gravity into two polishing lagoons.
Disinfection - Exiting the lagoons, wastewater enters a chlorine contact tank where it is disinfected with
a concentrated sodium hypochlorite solution. When the wastewater exits the contact tank a sodium
bisulfate solution is added for dechlorination.
Post -aeration — Following the chlorine contact tank the effluent flows through a rectangular weir where
flow is measured and then flows into a post -aeration tank where it is aerated as needed.
Effluent —The effluent is sampled upon leaving the post aeration tank and flows by gravity into Abbotts
Creek. A portion of the effluent is land applied onto 4 acres of trees on the plant site under permit
#WQ0016165.

Sludge Handling
All sludge is wasted from the BNR system into a diffused air flotation unit. Concentrated sludge from
this unit is pumped directly into four old anaerobic digesters. These digesters are presently used to
store and dewater (supernatant) the sludge before it is pumped to the compost facility. Supernatant
from these units is discharged directly to the head of the BNR System.
Concentrated sludge from these units is pumped directly to the plant's compost facility (WQ0001318).
All water removed from the sludge and from rainfall at this facility is contained and gravity flows directly
into the treatment plant's second stage lift station.
Sand is periodically removed from the BNR system and is used in the composting process.
The two polishing lagoons are periodically cleaned. The sludge from this is either pumped directly to the
compost facility or indirectly to the old digesters and then to the compost facility.
Sand (grit) from the plant's grit removal unit is hauled to the compost facility and is incorporated into
the compost process.

NC0055786
ATTACHMENT 5

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:2/1812013
Facility: Lexington
Laboratory:Aleritt Inc,�
x
Signatureof Imeylo
rin
x
Signature of Laboratory Supervisor
NPDES # NC00 55786
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Pipe #: 001 County: Davidson -�
Test Initiation Date/Time 2/5/2013 3:25 PM Avg Wt/Surv. Control 0.822
% Eff. Repl. 1 2 3 4
Control I Surviving #
Original #
Wt/original (mg)
15 1 Surviving #
Original #
Wt/original (mg)
10
8
8
9
10
10
10
10
0.736
0.574
0.771
0.783
10
10
8
10
10
10
10
10
0.820
0.611
0.732
0.719
% Survival 87.5
Test Organisms
r7 Cultured In -House
r7 Outside Supplier
Avg Wt (mg) 0.716 Hatch Date: 2/4/13
% Survival 95.0 Hatch Time: 3:00 pm CT
Avg Wt (m
g).F 0.721
F-36-7.1 Surviving #
% Survival 97.5
10
10
10
9
Original #
60 Surviving #
Original #
Wt/original (mg)
80 Surviving #
Original #
Wt/original (mg)
10
10
10
10
0.720
0.740
0.726
0.764
9
10
10
10
10
10
10
10
0.653
0.532
1 0.886
0.693
5
10
9
10
10
10
10
10
0.485
0.781
0.703
0.767
% Survival 97.5
Avg Wt (mg) 0.691
% Survival 85.0
Avg Wt (m
g).F 0.684
F-1-6-61 Surviving #
% Survival 92.5
Original #
Wt/original (mg)
Avg Wt (mg) 0.711
Water Quality Data
Day
Control
0
1
2
3
4 5 6
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) [nit/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
8.13 / 7.90
8.22 / 7.60
8.05 / 7.97
8.25 / 7.99
8.26 / 8:04
8.13 / 7.94
8.23 / 7.88
7.68 / 7.14
7.75 / 5.99
7.81 / 7.38
7.81 / 7.58
7.84 / 7.76
7.83 / 7.15
7.81 / 6.48
24.4 / 25.1
25.3 / 24.8
25.1 / 24.9
24.8 / 25.0
24.2 / 24.7
25.0 / 25.4
24.9 / 25.5
n 9 3 4 5 6
7.70 / 7.82
7.71 / 7.67
7.65 / 8.01
7.93 / 8.07
8.06 / 8.15
8.14 / 7.91
7.90 / 7.75
8.26 / 7.04
8.36 / 6.25
8.35 / 7.45
8.41 / 7.64
8.07 / T79
7.80 / 7.08
8.43 / 7.20
24.3 / 25.1
24.8 / 24.8
25.7 / 24.9
25.3 / 25.0
24.6 / 24.7
25.3 / 25.4
25.6 / 25.5
1 2 3
2/4/2013
2/6/2013
2/7/2013
24.00
24.00
24.00
64
64
66
63
63
67
357
377
386
<0.1
<0.1
<0.1
0.9
0.7
0.6
Dilution H2O Batch # 687 688
Hardness (mg/L) 47 46
Alkalinity (mg/L) 61 59
Conductivity (umhos/cm) 218 224
Survival
Growth Overall Result
Normal
NO
ChV >100
Hom. Var.
YES�r
NOEC
100
100
LOEC
>100
>100
ChV
>100
>100
Method
Steel's
Dunnet's
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
15
10 18
2.41-0.0635
30
10 18
2.41-0.3033
60
1 10 18
2.41 0.3526
80
10 18
2.41 0.4514
100
10 18
2.41 0.0670
8
10
10
9
10
10
10
10
0.657
0.758
0.757
0.673
DWQ Form AT-5 (1104)

Client: Lexington WWTP
NPDES #: NCOOSS786
Initiated by: 01—
Date/Time Fed: 2/5/13 11,10k ✓
Date/Time Born: 2/4/13 3:00 PM CT
Organism Source: Aquatox, Inc.
Transferred by:
Date
Time
Initials
Day 1
2/6
U/
�j1t�-r S"
Day 2
2/7,N�-
Day 3
2/8
10cu—
ih�—
Day 4
2/9
`�
Day 5
2/10
!Q'�M_
Day
Test Termination Data:C
Initials/Signature:
l
End Date: 2/12/13
End Time: q:w p"—
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10 Start Date: 2/5/13 Time: J�Sr
Test Vessel Size: 400 ml End Date: 2/ 12/ 13 Time: qZ Ja,—
Test Solution Volume: 250 ml
Temp. of Stock: �i''l °C Randomization: Yes No
# of Reps: 4 Incubator #:
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Fed by:
Date
Time 1
Initials
Time 2
Initials
Time 3
Initial
2/6
2/7
2/8
2/9
2/10
Sr
2/ 11 � 1 a- VIA^' I l:�l�r� / < 3. t ,
= Weekend, only 2 feedings needed

_ -��� AML I /TECH 1NC'_
�. y
` ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls_ /nc_
Chemical and Physical Determinations
Client: Lexington WWTP NPDES#: NCO055786 Start Date: 2/5/13 Time: ?t--
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 2/12/13 Time:
Day
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwate
Batch # :
/
c�
pH: Initial
Final
7, q
7, G
7 -q 7
D.O.: Initial
`�.CO�
�, 7S�
7 9/
2' i
Final
IL-1
5- •`1c,
7 3F
75-�
-
IT J
CF.gr
Temp.: Initial
Z ,
LS .
ZS !
Z
Z Z.
a,S-e
ZV-%
Final
7, �- �
�.� .�
't�5—• G
�. 7
?S
2, , 5
Conductivity: Initial
Z. j
'Z. ( f'j
�rl
Z? Z
Z1-8-
I
Final
Z33
(0
3i?�
Residual Chlorine:
40.t
<D. `
/-O.
Hardness:
IJ 7
2,
Alkalinity:
S-�
Day
Concentration: 15%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
6
Final
`7, Cs a�
, 7
9.00
?-q q
5i o
-1, q
D.O.: Initial
-7, -j Z,
- T 7-
'? �.,
?
7 oZ
-
Final
-7. 0�-2
.. �(�
7 . Co
7• �°1
..
Z3
6. �71
Temp. Initial
Zq.q
Final
2
r %
75—S
Conductivity: Initial
2 LID
Zqb
`2-u7
Z. /
:; 6C)
C:
Final
25(�'
Z F V
Z
41
_
Residual Chlorine:
/�O . I
LD
e 6 • 1
-. 0. 1
i 0• 1 I
LO . /
Day
Concentration: 30%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
D.O.: Initial
`1
%. 96
7. q �
.Q
7 Y 7
9 ,S-
E ("I
Final
�7,�1�
y�
'l,�75
7, &I
--?, j I
S 2-
Temp.: Initial
7 y
' S " o
5 . 1
Final
Z S
t
z
�, v
74, (
ZS
Conductivity: Initial
C;
Z7
Final
Z7
��J
%
`2✓7�
-G
Residual Chlorine:
L-0 <
4-0
Gb , I
-� L%
��'
L`•

MER/ TEC/Y, //V C"_
ENVI fZONMENTAL LABORATORIES^c
,u orvr:ro.. o, w..e,.r rcn.,orovr �.,cr ce.�r.ers. r
Chemical and Physical Determinations
Page 2 of 2
r
Client: Lexington WWTP NPDES#: NCO055786 Start Date: 2/5/13 Time: Zj
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 2/12/13 Time: q- ooL.-
Day 2 • ` -/
Concentration: 60%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
-7.
Final
^%•
7.7�
�.G2
,
a7,1Z
7, 7f"
D.O.: Initial
•1 U
', -03
1
9, l0
Final
L
�. 37
`). (, .•'•
�, / S�
�. L
Temp.: Initial
2-qlq,
Final
v
7`r. -7
Conductivity: Initial
Z q
�(D
Final
Residual Chlorine:
.4- C.
LG
Cp
L'C'
C C> (
L G, l
Z-G ,
Day
Concentration: 80%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
PH: Initial
"7.
-7, P3
7,7t
7 •
?
1 y-
Final
')� �/
, -71
' �
'K iH
-7,q (
7 5b
D.O.: Initial
Q
F•C)q
3
q
• I
�5•
Final
( .q V
Temp.: Initial
2 3
2 `� •�
a�
Z
Z 6
Final
j-•
a `�
(�
ay.
-2- =S'
Conductivity: Initial
3 2—&
-33C
L�
T 13q
�ffi�
Final
3
•7 3
Z-
~J 1
6
39S'
Residual Chlorine:
G.61. 1
< _
-4. l 1
/-C',t I
G 0 , I
• -D , l
Day
Concentration: 100%
0-1
1-2
2-3
34
4-5
5-6
6-7
Remarks
pH: Initial
7(�
(
_l ,�l 3
�,ElC
eU
Final
D.O.. Initial
7
g• q3
Final
Z,�
�•`��
7��
~��`�
-7.0
%,2o
Temp.: Initial
z r J
,5-, 7
Z
Final
'-2,
J
Conductivity: Initial
Final'
��
�j�� (�
3 ^ 3
q
Residual Chlorine:
G.Q , i
/_0 •
ZO ,ZLO
Hardness:
4
Alkalinity:
�, ��
r 17)
-�
l j

r
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls, Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lexington VWVTP
NPDES #: NCO055786
Date of Test: 2/5/13 C
Initial weights taken on: 3 - D 13
Page: 1 of 2
Outfall:
by: S 1 Organism: Pimephales promelas
Final weights taken on: 73 13by: S1�
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV
.°
U
A
B3�
b
>
A
gay ,
23L c�'
/ 0
0
D
I
30%
-.0
C
3�)S _ r;
�3Q,qc
T :
.
o Is33
;(L 1
`�'1)4U�cQ) I
1, 6 1-:1
Q �

HRITHC"j, ®ivc_
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Contro/s, /nc.
FISH LARVAL SURVIVAUGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lexington VWNTP
NPDES #: NCO055786
Date of Test: 2/5/13
Initial weights taken on: by:
Final weights taken on:by: ST
Page: 2 of 2
Outfall:
Organism: Pimepha/es promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
60%
B3
3 S5,
, 44
�/Sq
C Q0
o
�o 3
f L'
E : uq- �3
i,cz,
80%
A
B
� 5
040 -?J�
�3i. 5a
S,5�,55
T U3
c?
C) U3
D
g3, 33
Sl+-4. W
-4
%
Cc
100%
A
L's
0, 6,6 f
S�)

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lexington WWTP NPDES#: NCO055786 Start Date: 2/5113 Time:
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 2/12113 Time: l G�
Concentration Control
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
�)
Rep #2
Rep #3
Rep #4
d
y
Concentration
15%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1 — n
0
0
0
0
Rep #2
Rep #3
0_
Rep #4
Concentration 30%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
O�
Rep #2
G
r1
Rep #3
Rep #4
i�
`�

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lexington WWTP NPDES#: NC0055786 Start Date: 2/5/13 Time:
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 2/12/13 Time:
Concentration
60%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
0
C)
l
Rep #2
D
V
U
Rep #3
Rep #4
C)
0
C
�
V_C)
Concentration
80%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
'
y
Rep #2 _
C)
Rep #3
0
V
Rep #4
`J
Concentration
100%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
0
t
_
0—
Rep #2
O
v
v
Q)
Rep #3
`�_
/
0
Rep #4
�__

MERITECH, INC. Meritech Sample ID#:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txm rtech(cDbellsouth. net Web Site: www.meritech-labs.com
ILIE11 INFORMATION
Client: PO
Contact Person: 14 NPDES#: NC tO_5
Address:��-j1-(�.�'- C`<, IC- Phone: _j'J�j
City: Ji( Y-1 Pipe #: _ rl
Countyr .-1����1 StateZip: "7� 7 �_
Sample Site: h,{ A
Sample Type: ❑ Grab X Composite
SAMPLE INFORMATION
# of containers: 3
Sampling Time: StartDate: - LI 3 Start Time: -)�00 AM 'PM
End Date: Gi — — ) 3 End Time: PM - CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
**' SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLI
Collector's Name: Print: 'j, Signature:
TOXICITY TEST INFORMATION
�est Require hronic (7 days) Test Organism: xyz
Ceriodaphnia dubia (water flea)
El Acute (24-48 hours)
FWC: Z %
Comments:
Relinquished
Received
Relinquished
Received
Relinquished
Received by:
Relinquished by:
Received by:
AND W VERED WITH ICE ***
Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions)
ING INFORMATION
Date:
Date: 2 S
Date: 3`
Date: Z
Date: 2
Date:
Date:
Date:
Sample Temperature (°C)
Time:
,. (91
a PM
Time:
�1; C7 'pt
PM
Time:
/ 3 1
AM I&
Time:
3 f
AM
Time:
y
AM P
Time:
AM PM
Time
Time:
AM PM
AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
SAMPLE RECEIVING (Laboratory Use Onlvl
Relinquished by:
Received by: _� `c ;(�` Date: r' j Time: ; t{L A< APM-
Sample Temperatures (°C): ( I ) ( I I Sample Condition: C_P
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Merftech Sample ID #: d
Bioassay Sample Chain Of Custody
A� ' 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(&-bellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: w4PO#'
Q
NPDES#: NC T
Contact Person: L •e.
Address:
v
Phone:Z-�'�J
��
City: CJ1t.
��
Pipe #: �-
County: dC Stater Zip:
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑ Grab Composite # of containers:
Sampling Time: Start Date: �` �' �3 StartTime: -7. 0f) AM PM
End Date: �— 7 - 13 End Time: %.' c'O AM PM
CHRONIC TESTS SHOULD BE TAKEN
AFTER 10.00 AM IF AT ALL POSSIBLE
*zt to A.RtPr'..E CE��3TA s?LIERS A.-IR..E TO S Ct`»fvY LY T EL i' FULL tno a^1r S�J.-".: �%; �.i7esf .+? r {� � R2=�3 Z7'e f ivL �4w
Coector's Name: Print: 5-1 eijcn Signature:
Test Required:
TOXICITY TEST INFORMATION I
Chronic (7 days) Test Organism:Ve�rioclaphniadubia (waterflea)
Acute (24-48 hours) imephales promelas (fathead minnow)
❑ Mysidopsis Bahia (shrimp)
Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Date: 13
Time:
/ ' �vl�
AM
PM
Relinquished by:
Date: -Z-2 13
Time:
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Received by:
Date:
Time:
Q
AM
M
Relinquished by:
Date: —' — -�
Time:
L�
AM
CM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Received by:
Date: Time: AM PM
Date:
Sample Temperature (°C):
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
�rY iSS shipped 3tl F :£3e jar � dst be ? dE." and riji st be clearly I aAied for Saturday deliver'y.',_
Relinquished by: oc " 4( /
Date: -� L7 Time: � Sr `V AM
Received by:
Sample Temperatu s (°C): d ` i Sample Condition: C- `P cY
1r,,N (E = Laboratcrr copy .'El'_;J;;V =

MERITECH, INC. Meritech Sample ID #: O
Bloassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
` Phone: [phone] Fax:[phone]
Email: txmrtech(&bellsouth.net Web Site: www.meritech-labs.com
Client:
Contact Person:
Address:
City:
County:
Sample Site: L, 't• 'r
Sample Type: ❑ Grab
ri9lcl
V, Composite
State: I V 0
SAMPLE INFORMATION
PO#: t
NPDES#: NC
Phone:
^ Pipe #: a
l-� _ 9" /
Zip: %7% 7 i
# of containers: 3
Sampling Time: Start Date: oc - 7 - l3 Start Time: %• 0-0 (R)PM
End Date: 13 End Time: 700 PM - CHRONIC TESTS SHOULD BETAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLI
Collector's Name: Print: 5 fe u l jcl yr�C�.S Signature:
Test Required: (Chronic (7 days)
❑ Acute (24-48 hours)
Fwc: (� %
Comments:
VERED WITH ICE ***
TOXICITY TEST INFORMATION
Test Organism: Ceriodaphnia dubfa (water flea)
Pi ephales promelas (fathead minnow)
❑ Mysidopsis Bahia (shrimp)
Test Concentrations (if multiple dilutions):
SHIPPING INFORMATION
Relinquished by:
Date:
i�'�j ^ ) �
Z
Time: 2✓
PM
Received by:
Date:
'�
Time:? to-
PM
Relinquished by:
Date:
rr
Z O
Time: % Y
AM PM
Received by:
Date:
Time: -
AM PM
Relinquished by:
Date:
fy =/ -j
Time: 5—
AM PM
Received by:
Date:
Relinquished by:
Date:
Received by:
Date:
Sample Temperature (°C):
Time: AM PM
Time: AM PM
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: 61� r (rnL',��
Received by: Date: q, Time: AM PM
Sample Temperatures (°C): Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

Title: lexington
File: lexington Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.1809
W = 0.9610
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: lexington
File: lexington Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 9.3277 (p-value = 0.0967)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Title: lexington
File: lexington Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 5 0.0078 0.0016 0.1545
Within (Error) 18 0.1809 0.0101
----------------------------------------------------------------------------
Total 23 0.1887
----------------------------------------------------------------------------
(p-value = 0.9759)
Critical F = 4.2479 (alpha = 0.01, df = 5,18)
= 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title: lexington
File: lexington Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED
MEAN CALCULATED IN
SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
T STAT 0.05
------ ---
-------------------------
1
control
-----------
0.7160
------------------
0.7160
2
15
0.7205
0.7205
-0.0635
3
30
0.7375
0.7375
-0.3033
4
60
0.6910
0.6910
0.3526
5
80
0.6840
0.6840
0.4514
6
100
0.7113
0.7113
0.0670
----------------------------------------------------------------------------
Dunnett
critical value = 2.4100 (1 Tailed,
alpha = 0.05, df =
5,18)
Title: lexington
File: lexington Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
MIN SIG DIFF
% OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
(IN ORIG. UNITS)
CONTROL
-------
FROM CONTROL
------------
-----
1
--------------------
control
-------
4
----------------
2
15
4
0.1709
23.9
-0.0045
3
30
4
0.1709
23.9
-0.0215
4
60
4
0.1709
23.9
0.0250
5
80
4
0.1709
23.9
0.0320
6
----------------------------------------------------------------------------
100
4
0.1709
23.9
0.0047

Title: lexington - survival
File: lexsurvive Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.2700
W = 0.8697
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: lexington - survival
File: lexsurvive Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 9.6796 (p-value = 0.0848)
Data PASS Bl homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Fisher's Exact Test
----------------------------------------------------------------------------
----------------------------------------------------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION
--------------------------
DEAD ALIVE
TOTAL ANIMALS
---------------
CONTROL
5
35
40
15
----------------------------------------------------------------------------
2
38
40
TOTAL 7 73 80
----------------------------------------------------------------------------
----------------------------------------------------------------------------
Critical Fisher's value (40,40,5) (alpha=0.05) is 0.0. b value is 2.
Since b is greater than 0.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
----------------------------------------------------------------------------
----------------------------------------------------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION DEAD ALIVE TOTAL ANIMALS
-------------------------- ----- ----- ---------------
CONTROL 5 35 40
30 1 39 40
----------------------------------------------------------------------------
TOTAL 6 74 80
----------------------------------------------------------------------------
----------------------------------------------------------------------------
Critical Fisher's value (40,40,5) (alpha=0.05) is 0.0. b value is 1.
Since b is greater than 0.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
----------------------------------------------------------------------------
----------------------------------------------------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION DEAD ALIVE TOTAL ANIMALS
-------------------------- ----- ----- ---------------
CONTROL 5 35 40
60 1 39 40
----------------------------------------------------------------------------

TOTAL 6 74 80
Critical Fisher's value (40,40,5) (alpha=0.05) is 0.0. b value is 1.
Since b is greater than 0.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
IDENTIFICATION
--------------------------
CONTROL
Fisher's Exact Test
NUMBER OF
----------------------------------------
ALIVE DEAD
35 5
TOTAL ANIMALS
---------------
40
80 34 6 40
----------------------------------------------------------------------------
TOTAL 69 11 80
Critical Fisher's value (40,40,35) (alpha=0.05) is 28.0. b value is 34.
Since b is greater than 28.0 there is no significant difference
between CONTROL and.TREATMENT at the 0.05 level.
Fisher's Exact Test
NUMBER OF
----------------------------------------
IDENTIFICATION DEAD ALIVE TOTAL ANIMALS
-------------------------- ----- ----- ---------------
CONTROL 5 35 40
100 3 37 40
----------------------------------------------------------------------------
TOTAL 8 72 80
Critical Fisher's value (40,40,5) (alpha=0.05) is 0.0. b value is 3.
Since b is greater than 0.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Summary of Fisher's Exact Tests
----------------------------------------------------------------------------
NUMBER NUMBER SIG

GROUP
------------------------------
IDENTIFICATION
EXPOSED
DEAD 0.05
CONTROL
-----------
40
----------- -----
5
1
15
40
2
2
30
40
1
3
60
40
1
4
80
40
6
5
100
40
3

n ' I � I ►� y
N• I rnU'IiAwNF-� I O I Nrt
ct 1 I G 1 CD F-J
W I I ►tj I .. (D
! d I r
((DD
(D 1 I
(
LQ
(A I I H i FO-i (DD r
I I Plj I - is O
I I H I CO N
K I I I
(D I I I I
y I AF
F` 1 Q I H I F'• W
cti 1 tj
Qt. I O K C
F I F- F j I Z I
H- I OwrnLA) H0 1 I p N-
FJ 100a0cnF-i I I N C
(D 1 I
I O 1
l o0000o I Htt] I y
n 1 I Z 1 (D
I wwwkD 0 o I Ov I (A
cn I Nw-4-jw- I t-I I ct
1 m O m 0 O w I H I y
I OOOOoo z I Fj
I 1 H I
1 1 y , W
I , I-h
' 1 O
I I I
I NF-'NNN I ca I �•
l 0 W W W F-
I I
I Ul In o o U1 I I
o 0 0 0 0 I I x
' 1 O
I 1 ••
I I
H F-A F-I i m p 1 Q
I 00000 I H I 0
, 1 C'1'
I o0000 1 I Fj
o 0 0 0 0 1 1 0 z
FJ O
1 I n
I 1 I Fj
(D
I o000o I I trt �
l 0 0 0 0 0 I I
1 I (D O
I O 1
I I (J] 1 tt
I I O H I
1 I (n (7) I y
1 1 H
' 1 O
' z

Effluent To*it jI Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:5/17/2013
Facility: Lexinglon
Laboratory: Mi
NPDES # NC00 55786 Pipe #: 001 County: Davidson
Comments
x
Signature of Operator in sponsible Charge
x
Signature of Laboratory Supervi
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time
5/7/2013
3:45 PM
Avg WUSurv. Control 0.592
% Eff. Repl.
1
2
3
4
Control Surviving #
10
10
10
8
% Survival 95.0
Original #
15 Surviving #
Original #
Wt/original (mg)
10
10
10
9
10
8
9
10
10
10
10
0.557
0.603
0.538
0.505
Test Organisms
r Cultured In -House
fi* Outside Supplier
Hatch Date: 5/6/13
Survival 90.0 Hatch Time: 3:00 pm CT
Avg Wt (mg) 0.551
30 Surviving #
%Survival 90.0
9
10
10
Original #
Wt/original (mg)
Avg Wt (mg) 0.551
60 Surviving #
%Survival 97.5
Original #
WUoriginal (mg)
Avg Wt (mg) 0.570
80 Surviving #
%Survival 92.5
Original #
Wt/original (mg)
Avg Wt (mg) 0.596
100 Surviving #
%Survival 92.5
Original #
Wt/original (mg)
Avg Wt (mg) 0.571
Water Quality Data
Day
Control
o
t
2
3
a 5 s
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) IniUFin
High Concentration
pH (SU) IniUFin
DO (mg/L) IniUFin
Temp (C) IniUFin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
124.8
/ 25.2
0 1 2 3 4 5 R
7.64 / 7.90
7.80
/ 7.86
7.77 / 8.07
8.09
/ 7.88
7.80
/ 8.24
8.20
/ 7.95
7.90 / 7.77
8.53 / 6.86
8.07
/ 6.78
8.64 / 7.34
7.63
/ 7.20
7.99
/ 7.52
7.72
/ 7.18
8.01 / 7.12
25.5 / 25.2
24.9
125.8
25.8 / 24.2
24.2
/ 25.3
25.6
/ 25.0
24.9
/ 25.5
25.0 / 25.0
1 2 3
5/6/2013
5/8/2013
5/9/2013
24.08
24.00
24.03
60
60
64
62
63
65
352
345
356
<0.1
<0.1
<0.1
1.5
1.2
0.9
Dilution H2O Batch # 712 713 714
Hardness (mg/L) 48 48 48
Alkalinity (mg/L) 60 55 58
;onductivity (umhos/cm) 208 195 213
Survival
Growth Overall Result
Normal
YES
FII ChV 7100
Hom. Var.
YES
p71„;
NOEC
100
100
LOEC
>100
>100
ChV
>100
>100
Method
Dunnett's
Dunnet's
Stats
Survival
Growth
Conc.
Critical
Calculated
Critical Calculated
15
2.41
0.7682
2.41
0.1252
30
2.41
0.7682
2.41
0.1252
60
2.41
-0.3841
2.41
-0.3569
80
2.41
0.3841
2.41
-1.0143
100
2.41
0.3841
2.41
-0.3819

Client: Lexington WWTP
NPDES #: NCOO55786
Initiated by:
Date/Time Fed: 5/7/13
Date/Time Born: 5/6/13 3:00 PM CT
Organism Source: Aquatox, Inc.
Transferred by:
Date
Time
Initials
le -
Day 1
5/8
Day 2
5/9
Day
5/10
�''���.-
n-, S/
Day 4
5/ 11
Day 5
5/1 2
Day 6 5/13 �c� n' ^—
Test Termination Data:
Initials/Signature: '
End Date: 5/ 14/ 13
End Time:
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10
Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
Temp. of Stock: � °C
# of Reps: 4
Start Date: 5/7/13 Time:
End Date: 5/ 14/ 13 Time:
Randomization: G
No
Incubator #:
Fed by:
Date Time 1 Initials Time 2 Initials j Time 3 Initial
Day 5/7 .5� _ (� 12-%d/ __ —_ Y'c r) /Q1 2---
Day 1 5/8 Li M(V --- _ / � : c" ,
Day 2 5/ 9 51,z %l L/1& nlo�
Day 3 5/ 10 3 J-L-,
Day 5/11
Days 5/12
Day 6 5 / 13 � � .,,� ✓} '`—_ %E/,�.-� !' � 73 .'16Ptn y_
= Weekend, only 2 feedings needed

ENVIRONMENTAL LABORATORIES
' - A Division of vvater Technology and Controls, Inc -
Chemical and Physical Determinations
Client: Lexington WWTP KPDES#: NCO055786 Start Date: 5/7/13 Time: II
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 5/14/13 Time:
Day
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwate
Batch # :
/
pH: Initial:
Final
D.O.: Initial
ZO
g 3
,sue
c Z
'7�
9
`7.72
'.1
FinalLL
�. Z
;3
'7./-3 1
Temp.: Initial
L ��
Zy
2�.
?Y,3
2,
l
Z'
Final
2. 2,
Z /
7 �,
2S a
S
Conductivity: Initial
Z2(p
�. x
LIS,
9
Final
a3c
L4Q
rq,-
24 b
--Q,57
_
Residual Chlorine:
4 0-
:�-Q
zo.
L(;
Hardness:
Alkalinity:
Dav
Concentration: 15%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial9,08
Final
Z, t-1
�
S-T �
; (
�'�
% l 1i
,,?
�r; `�`
<f, )
v F /
v• ``,'-o
D.O. Initial
I
? 7
7,
—4.
24
Final
. 0 7
C
//l'
315�
�. jv
_�
�, t
-. Z y
%,
Temp. Initial
2yy2
q, b.
Final
2j Z
v
Conductivity: Initial
z b
c�1
Final
W—
�� %
a7�
'���I^
�
Z7 9
Residual Chlorine:
4 1),
�0.
C �'
Zo.
---o
Day
Concentration: 30%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
15
J
f
D.O.. Initial
�. ��
— c
? .Z��
7 A 'Y
Final
0
�. z
CC -
�;_ G
Temp.: Initial
2
z
2q. 3
Final
Zsr v
2S ,'�'
?�
_
1,
Conductivity: Initial
'767
Q14
Final
Ay-�,/
Qj-(c,
3)
Residual Chlorine:

MER/TECH, /NC
_ ENVIRONMENTAL LABORATORIES^c
..+ o!v/s/on of �Vn rs Tscnrse/o py nr�d co r o/s /
Chemical and Physical Determinations
Client: Lexington WWTP NPDES#: NCO055786 Start Date: 5/7/13
Test Organism: Pimephales prome/as Analyst(s): mer, MR, ST End Date: 5/14/13
Dav ST 5- k-;.
Page 2 of 2
Time: y �
Time: i l (J C -
1.1
Concentration: 60%
0-1
1-2
2-3
3-4
4-5
5-6
6-7 `' `
5' Remarks
PH: Initial
-7, %
. q
'� . �-�
G
7,� 3
)
�t'i•G�Z
Final
79
,$ ?
�81. OR
1 ,�'
1 75
g,C`U
D.O.: Initial
F ab
7.8,&
,
711
n,': 4 1-7,C/
(.
Final
-Yf
. 6�
7, t V
J.,SL
/6
7 3 1
Temp.: Initial
2 S
2
Z. 7
Zq
27r
Final
ZS L-S
2 L-, -L-
7 ,57. 3
Conductivity: Initial
Final
Residual Chlorine:
Z-0,
z-U,
LD,
Dav C/,fi 3
Concentration: 80%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
- Remarks
pH: Initial
70
7. 9&
'!
910 0
?�I a-
Final
-7,*
.k 7
7 -W
n p , z-�, t
9 7
%• 0
D.O.: Initial
$, Z 2-
-7.Ct 7
Q' (p
�/-
'_l(0'?
C(
Final
70
7 /i;
7. Z7
Temp.: Initial
Final
ZI�Z
�' r�
Z�
�.5, 3
2-
Conductivity: Initial
3
3
Final
Residual Chlorine:
z-,, ,
z-,-0
;
Dav
Concentration: 100%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
H: Initial
Final
'7, q V
IOR
7- TK
CK '
7. 7?.
D.O.: Initial
9-. Q
Fl 0 7
IF, (p
�
7 •
, �a
� G' �
Final
lb7
•3
7,�V
�;
-7, tc/
7 1 Z-
Temp.. Initial
,�
�N
2S-(�
7 q-�
J , �)
Final
Conductivity: Initial�j
S
Final
L
.�Sl
Lj
- Lo
Residual Chlorine:
��'.
<� .
�. i
G.0
Hardness:
L
;
Alkalinity:
e Z,
C--
�? 5

Y
WHNITHCHAV 1
ENVIRONMENTAL LABORATORIES
A Division of water TeChnoiogy and ContrO/5, Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lexington VWNTP
NPDES #: NCO055786
Date of Test: 5/7/13
Initial weights taken on:
Final weights taken on: 5 -
Page: 1 of 2
Outfall:
by: 5) Organism: Pimephales Promelas
by: 5-
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT./ORG. (mg)
SURV %
A
g3S: 5J9
(03
a
B
�
5�'15
5
o
C
O
c
8. ,15
C
5.
10
(0-5�
I5
D
3-,1-4
5 lS1
�} ' ��
b 0
q
0
B
D'�fl
5, ;
C : 6Cj5
q�.
U
Q,
`t
' ,V
9�
a
B
,a -
5 ►
�,
icy
CAS
Ito
o
D

WERITEC". ® e
ENVIRONMENTAL LABORATORIES
A Division or Water Technology and Controls. Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lexington VWWTP
NPDES #: NCO055786
Date of Test: 517/13
Initial weights taken on:-5
Page: 2 of 2
Outfall:
by: J-r Organism: Pimephales promelas
Final weights taken on: 5- I (a-'� by: <J 1
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT.IORG. (mg)
SURV %
so%
A
9
F--,�
B
c
�-t < .off'
�� ��
Cp ` �i
ld
60
D
e to . l�
i411 -5
� �
1)(�
80%io
a
3
�'-�@- .� I
q
Q. L� +
B�3.5
.�
I
70
4
� t
100 /o
A�.g
c.
D
.�� L)
�J
�i (LA I"r�9C1
i�v

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lexington WWTP
NPDES#:
NCO055786
Start Date:
5/7/13
Time:
Test Organism: Pimephales
promelas
Analyst(s):
mer, MR,
ST
End Date:
5/14/13
r
Time: tar",, -
Concentration Control
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
r' `1
V Rep #2
Rep #3
Rep #4
Concentration 15%
0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
'Day
Rep #1 V
`f
L—
1
T
0
l
V
Rep #2_
Rep #3
Rep #4
Concentration 30%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
Rep #2
Rep #3
lJ
Rep #4
V
i

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lexinqton WWTP NPDES#: NCO055786 Start Date: 5/7/13 Time: ���"
r
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 5/14/13 Time:
Concentration 60%
Re #1
P
Rep #2
Rep #3
Day 0
_
Day 1
v
Day 2
Daa�y�3
�/
y
/Day 4
y
�Day 5
y
�Day 6
Day 7
'/
l)
Rep #4
�
v
Concentration
80%
Rep #1
Rep #2
Rep #3
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
�
Day 7
y
Rep #4
Concentration 100%
Day 0
Rep #1
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
T
Day 7
Rep #2
Rep #3
Rep #4
'
v

MERITECH, INC. Meritech Sample ID#:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(Mbellsouth.netB Web Site: www.meritech-labs.com
Client: ') PO#:
Contact Person: NPDES#: NC Lao
Address: 1 j YT--.m Phone:
City: Pipe #:
County: State: ,A/L Zip: 112
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑ Grab 2Composite # of containers: 2
Sampling Time: Start Date: 5/ <O //3 Start Time: 6M� PM
End Date: 5 /,3 End Time: i'(}.S M) PM —� CHRONIC TESTS SHOULD BE TAKEN
6 AFTER 10:00 AM IF AT ALL POSSIBLE
***SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no airspace), CHILLED ND OVERED WITH ICE***
Collectors Name: Print: Signature:W
Test Required: Chronic (7 days)
❑ Acute (24-48 hours)
WC: &0 %
Comments:
TOXICITY TEST INFORMATION
Test Organism: Ceriodaphnia dubia (water flea)
- Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
l n SHIPPING INFORMATION
Relinquished by: ly� t1 )„ I - - _ Date: ! z? 'lam Time: _A9 PM
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Date:
Date:
Date:
Date:
Date:
Date:
Date:
Sample Temperature (°C)
Time: ' ; )v 4 (it PM
Time: AM
Time: AM
Time: 17(0 AM
Time: AM PM
Time: AM PM
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
��SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by: 2 *�
Received by: Date: 1 ) Time: J �� AM
�
cr �
Sample Temperatures, C): / �) / / Sample Condition: _:—t nq
WHITE = Laboratory copy YELLOW = Client copy

cl-
M E RITECH, 'I N C. Meritech Sample ID #: O's
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(Mbellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
s1Ak"I_T�3
Client: CPO#: 6-a-6
Contact Person: G•15Q,1,2 Cpy",.e, r- NPDES#: NC
Address: 1n W-e-s4 C4A41r C_5lr-r-Pt4 Phone: 51,te
City: 11f,4t A 4)4y- Vt I— Pipe #: Ok
County: Q ay.J 4;o V1 State: Y, C.... Zip:
_ SAMPLE INFORMATION
Sample Site: Ni
Sample Type: ❑ Grab Ltd' Composite # of containers:
f
Sampling Time: Start Date: J 13 Start Time: • b0 Ate. PM
End Date: End Time:, 00 AMl PM CHRONIC TESTS SHOULD BETAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED ND COV REED WITCH ICE ***
Collector's Name: Print: ����� �$�� Signature: ��
TOXICITY TEST INFORMATION
Test Required: Chronic (7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Comments:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
FWC: i2 % Test Concentrations (if multiple dilutions):
Date: S
Date:
Date:
Date: S
Date:
Date:
Date:
Date:
Sample Temperature (°C):
Time:
r',6
® PM
Time:
2.1b
(Aldf )PM
Time:
ABM
Time:
i
AM �1
Time:
2 3 0'
AM M '
Time:
AM PM
Time:
AM PM
Time:
AM PM
Method of Shipment: ❑ UPS ❑ Fed EXI ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
t
Relinquished by:
Received by: Date: I ! Time: 3 AM PM
Sample Temperature C): ��� / �� / / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Meritech Sample ID
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
.., Phone: [phone] Fax:[phone]
Email: txmrtech(cDbellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: O �- L-oPt )!L- PO#:
Contact Person: .NA (j&VATe NPDES#: NC (70SS1�
Address: cbl tC , Phone:
City: r ' 60 Pipe #:�_
County: QLao AS an State: 1/1 L Zip: Z7' ,q
SAMPLE INFORMATION
Sample Site: F fy(r (i�t�
Sample Type: ❑ Grab 1!( Composite # of containers:
Sampling Time: Start Date: 5� Start Time: T'44 AM PM
End Date: Q Ay End Time: f�Z AM PM — CHRONIC TESTS SHOULD BE TAKEN
11 AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLED AID COVEWITH ICE ***
Collector's Name: Print: `1 ��-� ��v�S�� Signature: M� 1A
TOXICITY TEST INFORMATION
Test Required: (] Chronic (7 days) Test Organism: � Ceriodaphnia dubia (water flea)
1L_J Acute (24-48 hours) V Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
[WC CO-0 % Test Concentrations (if multiple dilutions):
Comments:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
ate: S
A6 12
Timer% Q %
PM
ate:
Time: '72 69
PM
ate: S
P L
Time: 0 2 AM
ate: S
l o • Z
Time: AM
ate:
Time: 5 ` i (7 AM
Date:
Date:
Date:
Sample Temperature (°C):
Time:
Time
Time
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be Fed Ex and must be clearly labeled for Saturday delivery **
AM PM
AM PM
AM PM
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:�ob ,kZ-)
Received by: li f+,� �� �� �� Date:%1 p i Time: i AM PM
Sample Temperatures (°C): / / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

Title: lexington
File:' lexington Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.0574
W = 0.9742
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: lexington
File:' lexington Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 7.6757 (p-value = 0.1750)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Title: lexington
File:' lexington Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE
DF
SS
MS F
----------------------------------------------------------------------------
Between
5
0.0061
0.0012 0.3829
Within (Error)
18
0.0574
0.0032
----------------------------------------------------------------------------
Total
23
0.0635
----------------------------------------------------------------------------
(p-value = 0.8540)
Critical F =
4.2479 (alpha =
0.01, df = 5,18)
=
2.7729 (alpha =
0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title: lexington
File:' lexington Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED
MEAN CALCULATED IN
SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
------------------
T STAT 0.05
------
-----
1
--------------------
control
-----------
0.5558
0.5558
2
15
0.5508
0.5508
0.1252
3
30
0.5508
0.5508
0.1252
4
60
0.5700
0.5700
-0.3569
5
80
0.5963
0.5963
-1.0143
6
100
0.5710
0.5710
-0.3819
----------------------------------------------------------------------------
Dunnett critical value = 2.4100 (1 Tailed,
alpha = 0.05, df =
5,18)
Title: lexington
File: lexington Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
MIN SIG DIFF
% OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
(IN ORIG. UNITS)
----------------
CONTROL
-------
FROM CONTROL
------------
-----
1
--------------------
control
-------
4
2
15
4
0.0962
17.3
0.0050
3
30
4
0.0962
17.3
0.0050
4
60
4
0.0962
17.3
-0.0143
5
80
4
0.0962
17.3
-0.0405
6
----------------------------------------------------------------------------
100
4
0.0962
17.3
-0.0152

Title: lexington - survival
File:' lexsury Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.1525
W = 0.9193
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: lexington - survival
File: lexsury Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 4.1885 (p-value = 0.5226)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Fisher's Exact Test
__-------------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
-------------------------- ----- ----- --------
CONTROL 38 2 40
21 36 4 40
--------------------------------------------------------------------
TOTAL 74 6 80
Critical Fisher's value (40,40,38) (alpha=0.05) is 32.0. b value is 36.
Since b is greater than 32.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
--------------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
-------------------------- ----- ----- ---------
CONTROL 38 2 40
42 36 4 40
--------------------------------------------------------- ------------------
TOTAL 74 6 80
Critical Fisher's value (40,40,38) (alpha=0.05) is 32.0. b value is 36.
Since b is greater than 32.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
---------------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION DEAD ALIVE TOTAL ANIMALS
----- --------------------
--------------------------
CONTROL
2
Wf
40
60 1 39 40
--------------------------------------------------------------------

TOTAL 3 77 80
Critical Fisher's value (40,40,2) (alpha=0.05) is negative. b value is 1.
no significant difference
Fisher's Exact Test
_____-------------------------------- NUMBER OF
----------------------------------------
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
-------------------------- ----- ----- ---------------
CONTROL 38 2 40
80 37 3 40
----------------------------------------------------------------------------
TOTAL 75 5 80
Critical Fisher's value (40,40,38) (alpha=0.05) is 32.0. b value is 37.
Since b is greater than 32.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Fisher's Exact Test
----------------------------------
NUMBER OF
----------------------------------------
IDENTIFICATION ALIVE DEAD TOTAL ANIMALS
-------------------------- ----- --------------------
CONTROL 38 2 40
100 37 3 40
----------------------------------------------------------------------------
TOTAL 75 5 80
Critical Fisher's value (40,40,38) (alpha=0.05) is 32.0. b value is 37.
Since b is greater than 32.0 there is no significant difference
between CONTROL and TREATMENT at the 0.05 level.
Summary of Fisher's Exact Tests
----------------------------------------------------------------------------
NUMBER NUMBER SIG
GROUP IDENTIFICATION EXPOSED DEAD 0.05

- - - -
- - -c- - - - - - - - - - - - - - - - - - - - - - - -
- - - - - - - - - -
CONTROL
40
2
1
21
40
4
2
42
40
4
3
60
40
1
4
80
40
3
5
100
40
3

Title': lexington
- survival
File: lexsury
Transform:
NO TRANSFORMATION
------------------------------------------------------------------------
ANOVA Table
--
SOURCE
DF
SS
MS F
---
------------------------------------------------------------------------
Between
5
0.0171
0.0034 0.4033
Within (Error)
18
0.1525
0.0085
----------------------------------------------------------------------------
Total
23
0.1696
----------------------------------------------------------------------------
(p-value = 0.8402)
Critical F =
4.2479 (alpha =
0.01, df = 5,18)
=
2.7729 (alpha =
0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title: lexington - survival
File:" lexsury Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
--------------------------------------------------
TRANSFORMED
MEAN CALCULATED IN
SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
--------------
T STAT 0.05
-----
1
--------------------
control
-----------
0.9500
0.9500
2
21
0.9000
0.9000
0.7682
3
42
0.9000
0.9000
0.7682
4
60
0.9750
0.9750
-0.3841
5
80
0.9250
0.9250
0.3841
6
100
0.9250
0.9250
0.3841
--------------------------------------------------
Dunnett critical value = 2.4100 (1 Tailed,
alpha = 0.05, df =
5,18)
Title:
lexington - survival
File:
lexsury
Transform:
NO
TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
-----------------------------------------------------------
NUM OF
MIN SIG DIFF
% OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
-------
(IN ORIG. UNITS)
----------------
CONTROL
----
FROM CONTROL
-----
1
--------------------
control
4
2
21
4
0.1569
16.5
0.0500
3
42
4
0.1569
16.5
0.0500
4
60
4
0.1569
16.5
-0.0250
5
80
4
0.1569
16.5
0.0250
6
-----------------------------------------------------------
100
4
0.1569
16.5
0.0250

Effluent Toxicity R o Form -Chronic Fathead Minnow Multi -Concentration Test Date:8/16/2013
Facility: Lexington
Laboratory: Meritech, Inc.
x
of Cep ,gtor "esponsible Charge
x r//!
Signature of Laboratory Supervisor
NPDES # NC00 55786
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Pipe #: 001 County: Davidson
Test Initiation Date/Time 8/6/2013 2:30 PM Avg Wt/Surv. Control 0.776
% Eff. Repl. 1 2 3 4
1controll Surviving #
Original #
Wt/original (mg)
10
10
1 10
10
10
10
10
10
0.781
0.703
0.669
0.951
15 Surviving #
% Survival 100.0
Original #
Wt/original (mg)
Avg Wt (mg) 0.832
10
10
10
10
10
10
10
10
0.730
0.802
0.870
0.925
% Survival 100.0
Avg Wt (mg) 1 0.776 1 Hatch Date: 815/13
Hatch Time: 3:00 pm CT
30 Surviving #
% Survival 90.0
Original #
Wt/original (mg)Avg
Wt (mg) 0.705
60 Surviving #
% Survival 100.0
Original #
Wt/original (mg)
Avg Wt (mg) 0.865
80 Surviving #
% Survival 97.5
Original #
Wt/original (mg)
Avg Wt (mg) 0.806
100 Surviving #
% Survival 97.5
Original #
Wt/original (mg)
Avg Wt (mg) 0.781
Water Quality Data
Day
Control
0
1
2
3
4 5 6
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp. at Receipt (°C)
9
8
9
ffO.
10
10
10
3
0.704
0.655
0.698
10
10
10
10
10
10
10
10
0.789
0.928
0.909
0.833
9
10
10
10
10
10
10
10
0.761
0.916
0.734
0.811
10
9
10
10
10
10
10
10
0.677
0.742
0.905
0.801
8.16 J 7.99
8.21 / 7.58
7.95 / 7.83
7.96 / 7.99
8.08 ! 7.97
8.05 / 7.86
7.99 / 7.67
7.57 ! 7.10
7.57 / 6.60
7.57 / 7.27
7.59 / 7.56
7.86 / 7.58
7.75 / 7.24
7.69 / 6.01
24.9 / 25.0
25.1 / 25.0
24.5 / 24.9
25.0 / 25.1
24.8 / 25.1
24.8 ! 25.0
24.7 / 25.0
0 1 2 3 4 5 e
7.64 / 7.87
7.73 / 7.44
7.47 / 7.65
7.60 / 7.91
7.92 ! 7.88
7.86 / 7.79
7.67 / 7.48
8.03 / 6.82
7.83 / 6.36
8.01 / 7.09
7.79 / 7.60
7.69 / 7.45
7.63 / 7.32
7.88 / 6.25
24.8 / 25.0
24.9 / 25.0
25.2 / 24.9
25.6 / 25.1
24.3 / 25.1
24.8 / 25.0
25.3 / 25.0
1 2 3
8/5/2013
8/7/2013
8/8/2013
24.00
24.00
24.00
68
68
70
51
56
57
406
424
405
<0.1
<0.1
<0.1
2.3
1.3
1.7
Dilution H2O Batch # 737 738 739 740
Hardness (mg/L) 44 46 46 42
Alkalinity (mg/L) 54 58 52 53
conductivity (umhos/cm) 215 193 213 198
Test Organisms
r Cultured In -House
Outside Supplier
Survival
Growth Overall Result
Normal
Slats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
15
10 18
2.41-0.9108
30
10 18
2.41 1.1599
60
10 18
2.41-1.4499
80
10 18
2.41-0.4819
100
10 18
2.41-0.0858
rnn/n Fn,m ar_A /11fel

Client: Lexington
NPDES #: NCOOSS786
Initiated by:
Date/Time Fed: 8/6/13
Date/Time Born: 8/5/13 3:00 PM CT
Organism Source: Aquatox, Inc.
Transferred by:
Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber: 10 .. Start Date: 8/6/ 13
Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
Temp. of Stock: ?. 'd °C
# of Reps: 4
Date
Time Initials
Day 1
8/7
r
a--
Day 0
Day 2
8/8�
„----��^- 1
—T-
Day 1
Day 3
8/9
926, `2u,, —
Day 2
Day 4
8/ 10
(.ww }dam^-
Day 3
Day 5
8/ 11
(l�♦ its'
J�..
Day 4
r
Day 6
8/ 12
;i7 A- t -
Day 5
Day 6
Test Termination Data: f
Initials/Signature:],
End Date: 8/13/13
End Time:
End Date: 8/13/13
Fed by:
Time: U-
Time: C;
Randomization: Yes No
Incubator #: 'I
Date
Time 1 Initials
Time 2
Initials
Time 3
Initial
8/6
8/7
8/8
8/9
8/10
8/12
LlAd
= Weekend, only 2 feedings needed

ENV IROLAT NMENTAL ABORORIES
i A Division of Water Technology and Controls, /nc_
Chemical and Physical Determinations
Client: Lexington f;PDES#: NCO055786 Start Date: 8/6/13 Time:
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 8/13/13 Time: ft' (�l e,-•
Day
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwater
Batch # :
'
^^�
pH: Initial
.11 n
j
i . 9�
7�((kk
�1
79 %
Final
i'.`�`
�� S
i . $ 3
'7-1-1 `
`7- � 7
2 Sk
7, �
D.O.. Initial
7S 7
7
�. t"
% ?S-
Final
.(,a 0
�. Z-7
?: Svc
7. Z `i
U
Temp.: Initial
? 4,1
�—j
- 4.1
Z� ,
Final
',�
�..5
y<y
Z,S
L? ;�
'?0
-7
Conductivity: Initial
?� j
I �~ 3
1
Final
=' �``�
i���
,cL
Residual Chlorine:
C , I
f-'r✓', i
'�` ,
-=� /
L,
�L.-
Hardness:
1H
Alkalinity:
Day
Concentration: 15%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
73
7
Final
7• `�
�.?�
%,�
D.O.. Initial
—;
-%
r/�`�'
7G b
7, �00
C
-), 7-7.
7 0
Final
�; , >, (
�{
7. i (
Temp.. Initial
Finals
, (,'
t'
Conductivity: Initial
Z
1-
Final
Residual Chlorine:
n
Day
Concentration: 30%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
l.if��;
?'':� �`
7
3
1`j.'S
�•.�
Final
'.b>'
7, 3
1'3
7,' 3
D.O.: Initial
^'. r. L
'
% 70
7
7/
-7•-7U
Final
-%- �,�
Temp.. Initial
' �� r
?? -r
:2 . 7f
,Iq
Final
-2,
Conductivity: Initial
,��
�r lC
2-7
FinalR�
Residual Chlorine:
L(;
��` !
C' .
'^ • I
C. !
G,C'

Client: LexinMon
IWA=—Jlr,f TECH, ff ~ C_
ENVI MC)"Mar=rJ-rAt- 1-^13C3FZ,-1'F-CD- I= -
Chemical and Physical Determinations
NPDES#: NCO055786 Start Date: 8/6/13
Test Organism: Pimephales promelas Analyst(s): mer, MR, ST End Date: 8/13/13
Day
Page 2 of 2
Time:
Time:
Concentration: 60%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
PH: Initial
7.
7 .7'
?- (_0 1
-7, 7
Final7.
-7,q7
'?.(o7
—7
D.O. Initial
7V?
7.
Final
�
7.
7 csj'
Temp.: Initial
V-
z I I "
Final
17157 111�
L17-
Conductivity: Initial
-,,
3
A3
3C�3
1
3&-�-
Final
-J3
76 3
339
3 t—Q
Residual Chlorine:
b
C , I
/— , . ,
. ,
Z- 0 f I
1<0
Day
Concentration: 80%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
PH: Initial
7.67
7�-'
72�
Final
TC
-7-0
/1
-7,
-7,5--3
D.O.: Initial
7V
7.?2-
Final
Ll
-,,C)
Temp.: Initial
2-
-z4
—7 I/A
Final
7
Conductivity: Initial
5'
Final
--1
'7
Residual Chlorine:
Z
o
Day
Concentration: 100%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
7. (o
Final
J.
7q9
D.O.: Initial
3-;
.01
ld
Final
i 6
E
�
6.2
Temp.: Initial
-1
-2 01'
z5,3
Final
Is,
2-
Conductivity: Initial
c--ko
Lf b ET
�-4
Final
Residual Chlorine:
Hardness:
Alkalinity:
5
5
57

MHRITHC"J, Irvc.
ENVIRONMENTAL LABORATORIES
A Division of Water Technology and Controls, /—
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: _ Lexington
NPDES #:. NCO055786
Date of Test: 8/6/2013
Initial weights taken on: by:
Final weights taken on: )+ 2) by: D I
Page: 1 of 2
Outfall:
Organism: Pimephales promela
PAN WT.
PAN + ORG.
WT. OF ORG.
MEAN
CONC
REP
(mg)
WT. (mg)
(mg)
# ORG.
WTJORG. (mg)
SURV %
A
•�C,
-6
B
0
C
L I
L", Lki-111c,
D
�4
A
B
Uu
15%
C
�•JD
A
F-
B
30%
C
J0
L-1
D

A4HRJ1 TA=-Cmv 1j%f C.
ENVIRONMENTAL LABORATORIES
A Division of In Technology and Controls, /,c.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lexington
NPDES #: NCO055786
Date of Test: 816113
Initial weights taken on:,",
Final weights taken on: 14 - i'7)
Page: 2 of 2
Outfall:
by: Organism: Pimephates promela
by:
CONC
REP
PAN WT.
(Mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(Mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
60%
A
6-)
B
S
L
C
L
D
80%
A
B
Cj
C
--a
D
02i
100%ff
A
B
q0
C
uj
D

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client:
Lexington
NPDES#:
NCO055786
Start Date:
8/6/13
Time:
Test Organism:
Pimepha/es
promelas
Analyst(s): mer, MR,
ST
End Date:
8/13/13
Time:
Concentration
Control
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
!
�,j
C)
0
L)
f.
`v
Rep #2
Rep #3
C%�_
J,
Rep #4
Concentration
15%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
Rep #2
Rep #3
G
v
}
J
Rep #4
u
Concentration
30%
Day 0
Day 1
Day 2
Day 3
Day 4
Day 5
Day 6
Day 7
Rep #1
t�
Rep #2
Rep #3��,I
Rep #4
t

MERITECH, INC.
Mortality
Data: Chronic Fathead Test
Client: Lexington
NPDES#:
NCO055786
Start Date:
8/6/13
Time: 74`.; --
r
Test Organism: Pimephales
promelas
Analyst(s): mer, MR, ST
End Date:
8/13/13
Time:
Concentration 60%
Day 0
Day 1
Day 2
Day 3 Day 4
Day 5
Day 6
Day 7
Rep #1
(.J (/!
```
�1
Rep #2
Rep#3
Rep #4
Concentration 80%
Day 0
Day 1
Day 2
Day 3 Day 4
Day 5
Day 6
Day 7
Rep #1
rr
I I
%�
i r%
I J'
Rep #2
.�
f i
c)
Rep #3
Rep #4
Concentration 100%
Day 0
Day 1
Day 2
Day 3 Day 4 Day 5
Day 6
Day 7
Rep #1
Rep #2 C"
Rep #3
1
�`
Rep #4-``��

MERITECH, INC. Meritech Sample ID#:
Bioassay Sample Chain of Custody c
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(cDbellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: Cjlt") of �ettl }t, PO#:
Contact Person: Co1MQi- NPDES#: NC
Address: as G.M41 SATf A Phone:
City: [=Ct,4&k Pipe #: _
County: Q(41A&1Y) State: (o C " Zip: *7 2
- SAMPLE INFORMATION
Sample Site: r i l l e_; t
Sample Type: ❑ Grab 9 Composite # of containers: C—
Sampling Time: Start Date: :� " 2j Start Time: 7: C'V% PM
End Date: �� (c7 End Time: ? C�' (� AM PM —� CHRONIC TESTS SHOULD BETAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COrKPLETELY FULL (no airspace), CF;ILLED ► Cl
�J
�j� —7
Collector's Name: Print: 'J i e v E� C t y}- �' S Signature:
Test Required: echronic (7 days)
❑ Acute (24-48 hours)
WC: V
Comments:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
TOXICITY TEST INFORMATION
WITH ICE ***
Test Organism: ❑ Ceriodaphnia dubia (water flea)
V' Pimepha/es promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
PPING INFORMATION
Date:
- 40-
Date:
Date:
O49 13
Date:
- /
Date:
- 4 `13
Received by:
Date:
Relinquished by:
Date:
Received by:
Date:
Time:
; 0- AM
PM
Time:
7'; `r'C7
PM
Time:
�/� 0 PM
Time:
! l ��i kd_V
PM
Time:
AM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
'* Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
SAMPLE RECEIVING (Laboratory Use Only)
�n
Relinquished by: � ` � f> �- 4 j s � � •� f� ���
' I `
Received b r i"\ 1 Date: ,'w C AM PM
Y r: (, f 1 _� Time:
Sample Temperatures (°C): / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. Meritech Sample ID #: 4 �L!
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(cDbellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: CI+1/1 0C_ 1.0 ,v,l 441'1 PO#:
Contact Person: —61 �04/klt uyl_ NPDES#: NC QQ.SS'-� M
Address: zw L C,C„� c rat/. Phone: 6b
City: -
-- II Pipe
116j
County: 1 " U1 w) State: Zip: .Q D-Z n L
SAMPLE INFORMATION
Sample Site: � �� t (,� (�
Sample Type: ❑ Grab VComposite # of containers:_
Sampling Time: Start Dater ?- Start Time: i; 6 D AM PM
End Date: — —) 3 End Time: % .Q D rAk3) PM —► CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
*** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CHILLS D C VERED WITH ICE ***
Collector's Name: Print: (�{�{ q V G� np5 Signature:
t
� TOXICITY TEST INFORMATION
Test Required: `LCJ Chronic (7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑�❑ Acute (24-48 hours) Qimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
FWC: Test Concentrations (if multiple dilutions):
Comments:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
PPING INFORMATION
Date: _� - ) 3
Date:
Date:
Date:
Date:
Date: _
Date:
Date:
Sample Temperature (°C):
Time:
7; o(
PM
Time: ' m 44
Time!w1;
PM
A�I PM
Time:
Time:
yf
(a (PM
AM /M;
Time:
AM PM
Time: AM PM
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery **
Relinquished by: Q j
11
Received y: Date: Time: u ' ` ' ,
. AM
Sample Temperatures (°C): . / i` J / / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MIERITECH, INC. Meritech Sample ID#: �' ?y� C` (J � L
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Email: txmrtech(a-)bellsouth.net Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: PO#:
Contact Person: NPDES#: NC OkZdT
Address: 2N V-3-CA E`ap,,,� 1; 2. % Phone: 35(A
City: Pipe #:.61
County: State: L Zip:
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑Grab rw
LN Composite # of containers:
Sampling Time: Start Date: - f; 3 Start Time: PM
End Date: End Time: 7 (AM PM —+ CHRONIC TESTS SHOULD BE TAKEN
AFTER 10:00 AM IF AT ALL POSSIBLE
** SAMPLE CONTAINERS ARE TO BE COMPLETELY FULL (no air space), CFIILLE VERED tNITH ICE *"
Collector's Name: Print: Signature:
TOXICITY TEST INFORMATION
Test Required: Chronic (7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
Acute (24-48 hours) Ij Pimephales promelas (fathead minnow)
c �❑ Mysidopsis bahia (shrimp)
[WC: �Y i % Test Concentrations (if multiple dilutions):
Comments:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Relinquished by:
Received by:
Date:
Date:
Date: C1
Date:--
Date: Y - 15 , �o
Date:
Date:
Date:
Sample Temperature (°C):
Time: -7;0(J
AM PM
Time: a±5 _
PM
Time: 1
AM
Time: f�
AM
Time: l
AM
Time:
AM PM
Time: AM PM
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery "*
SAMPLE RECEIVING (Laboratory Use Only)
i
Relinquished by:
Received by: �_ 11` ��_ Date: 4 j ` } Time: `} AM PM
Sample Temperatures (°C): `.� / ( 7 / / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

Title: lexington
File: lexington Transform: NO TRANSFORMATION
Kolmogorov Test for Normality
----------------------------------------------------------------------------
D = 0.1095 (p-value > 0.100)
D* = 0.5543
Critical D* = 1.035 (alpha = 0.01 , N = 24)
= 0.895 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: lexington
File: lexington Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 3.0179 (p-value = 0.6972)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Title: lexington
File: lexington Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 5 0.0599 0.0120 1.5982
Within (Error) 18 0.1349 0.0075
----------------------------------------------------------------------------
Total 23 0.1948
----------------------------------------------------------------------------
(p-value = 0.2111)
Critical F = 4.2479 (alpha = 0.01, df = 5,18)
= 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title: lexington
File: lexington Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED
MEAN CALCULATED IN
SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
T STAT 0.05
-----
1
--------------------
control
-----------
0.7760
------------------
0.7760
------ ---
2
15
0.8317
0.8317
-0.9108
3
30
0.7050
0.7050
1.1599
4
60
0.8647
0.8647
-1.4499
5
80
0.8055
0.8055
-0.4819
6
100
0.7813
0.7813
-0.0858
----------------------------------------------------------------------------
Dunnett critical value = 2.4100 (1 Tailed,
alpha = 0.05, df =
5,18)
Title: lexington
File: lexington Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
MIN SIG DIFF
% OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
(IN ORIG. UNITS)
CONTROL
FROM CONTROL
-----
1
--------------------
control
-------
4
----------------
-------
------------
2
15
4
0.1475
19.0
-0.0557
3
30
4
0.1475
19.0
0.0710
4
60
4
0.1475
19.0
-0.0887
5
80
4
0.1475
19.0
-0.0295
6
----------------------------------------------------------------------------
100
4
0.1475
19.0
-0.0052

Title: lexington - survival
File: lexsury Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.0350
W = 0.7605
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: lexington - survival
File: lexsury Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.

Title:
lexington - survival
File:
lexsury
Transform:
NO
TRANSFORMATION
Steels Many -One Rank Test
- Ho:
Control<Treatment
----------------------------------------------------------------------------
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
VALUE
DF 0.05
-------------------------
1
control
-----------
1.0000
-------
------
----- ---
2
15
1.0000
18.00
10.00
4.00
3
30
0.9000
12.00
10.00
4.00
4
60
1.0000
18.00
10.00
4.00
5
80
0.9750
16.00
10.00
4.00
6
100
0.9750
16.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed
( k = 5 )