HomeMy WebLinkAboutNCD003446721_19900118_Celeanse Corporation - Shelby Fiber_FRBCERCLA SAP QAPP_Groundwater Monitoring - Analytical Data-OCRI
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Westinghwse Environmental
and Geotechnical Services. Inc.
January 4, 1990
U. s. Environmental Protection Agency
345 Courtland Street, N.E.
Atlanta, Georgia 30365
ATTENTION: Ms. Michelle Glenn
Remedial Project Manager
4000 DeKalb Technology Parkway, NE
Suite 250
Atlanta, Georgia 30340
(404) 458-9309
FAX (404) 458-9438
SUBJECT: Request for Sampling Notification Variance
Groundwater Treatment System Upset Evaluation
Operable Unit 1 Remediation
Hoechst Celanese Facility
Shelby, North Carolina
Westinghouse Project 4124-85-0S0H
Document Control 85050H-202
Dear Ms. Glenn:
During late December 1989, an increase was noted in the COD and
ammonia-nitrogen of the inner tier combined influent. As a
result, EPA was verbally notified on December 28, that an
additional sampling of the influent would be made on January 3,
1990, to measure the influent quality and to try and identify
the source of the COD and ammonia increase. During the period
between December 28 and January 2, the SBR effluent quality
continued to deteriorate, and microscopic evaluation of the
mixed liquor on January 2 showed no biological activity. Thus,
pumping of the inner tier system was suspended, and the sampling
planned for January 3, 1990 was cancelled. The SBR was reseeded
with about 2,000 gallons of municipal sludge from Boiling
Springs, North Carolina, and Hoechst Celanese plans to restart
the system on January 8, 1990.
During our telephone conversation on January 3, Westinghouse
requested EPA approval to waive the 48-hour sampling
notification provision in the consent decree to have the
flexibility to sample as needed when problems in the SBR are
apparent. It was our understanding that this request was
approved, but that written documentation of the request was
required.· This letter constitutes our request for the sampling
notification waiver. As with the routinely scheduled sampling,
EPA will be provided copies of all data.
5050H171
A Westinghouse E!ectric Corporation subsidiary
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Request for Sampling Notification Variance
Groundwater Treatment System Upset Evaluation
Operable Unit 1 Remediation
RC/Shelby, North Carolina Facility
Westinghouse Project 4124-85-0SOH
Document Control 85050H-202
Page 2
Based on our understanding that our request was approved,
Hoechst Celanese plans to implement daily COD monitoring of the
SBR influent at the equalization tank and at a point between the
metals precipitation unit and the SBR. This monitoring will be
in addition to the daily COD monitoring presently performed. In
addition, other types and frequencies of both operational and
investigatory analyses may be performed as needed. The results
of these analyses will be reported in the normal format.
We are initiating the expanded sampling and analysis program
with the restarting of the SBR scheduled for January a, 1990.
If we have misunderstood your tentative approval of our request,
please contact us immediately. If we have not heard from you by
January 10, 1990, we will assume that you are in agreement with
our request to waive the sampling notification requirement on an
as-needed basis to investigate operational problems within the
treatment system.
Thank you for your timely consideration of our request.
Very truly yours,
WESTINGHOUSE ENVIRONMENTAL AND GEW\Ec[rL ffl;;Es, INC.
M. Kirk Mays, P.E.
Senior Environmental Engineer
C.a (.l;_,\.J.._t/:. lU bf!.eciu-, , ~
Everett W. Glover, Jr. P.E.
Project Manager
MKM/EWG/pys
cc: Ron Caldwell-RC/Shelby
Bill Carter-RC/Shelby
John McBride-RC/Shelby
Archie Pittman-RC/Shelby
5050H171
Jim Pullen-RC/Charlotte
Jack Kelley-RC/Shelby
Terry Atkins-RC/Shelby
earl Burrell-Davis & Floyd/Grnwd
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Westinghoose Environmental
and GIIIIEchnical Services, Inc.
January 5, 1990
U. s. Environmental Protection Agency
345 Courtland Street, N.E.
Atlanta, Georgia 30365
ATTENTION:
SUBJECT:
Ms. Michelle Glenn
Remedial Project Manager
SBR Operational Evaluation
Hoechst Celanese Facility
Shelby, North Carolina
Westinghouse Project 4124-85-050H
Document Control 85050H-203
Dear Ms. Glenn:
4000 DeKalb Technology Parkway. NE
Suite 250
Atlanta. Georgia 30340
(404) 458-9309
FAX (404) 458-9438
As generally discussed in our Document Number 85050H-202, dated
January 4, 1990, there have been additional problems maintaining
adequate'biological activity in the SBR. Thus, Hoechst Celanese
is initiating a study to look more closely at the conditions
occurring in the SBR, and at the historical conditions that
occurred prior to and during the times that the SBR biomass
died. This study will be a collaborative effort between Hoechst
Celanese, Westinghouse and Davis and Floyd, Inc. Davis and
Floyd, Inc. designed and provided consulting services on the
operation of the wastewater treatment plant for the Shelby
facility and is very familiar with biological treatment of
textile wastewaters. In addition, Davis and Floyd's laboratory
QA/QC manual has already been sent to EPA for review since they
provided analytical service for the RI and are a secondary
source of analytical support for the remediation. A general
description of the tasks involved in the study will be provided
to EPA when it has been compiled.
5050H172
A Westinghouse Electric Corporation subsidiary.
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, SBR Operational Evaluation
HC/Shelby, North Carolina Facility
Westinghouse Project 4122-85-0SOH
Document control 85050H-203
Page 2
If you have any questions about this letter, please contact us.
Very truly yours,
WESTINGHOUSE ENVIRONMENTAL AND
GEOTECHNICAL SERVICES, INC.
~ ~ fvt~s
M. Kirk Mays, P.E.
Senior Environmental Engineer
'G,lu__,_,_Jf_ U), ~tkt ,~
Everett w. Glover, Jr. P.E.
Project Manager
MKM/EWG/pys
cc: Ron Caldwell -HC/Shelby
Bill Carter -HC/Shelby
Jack Kelley -HC/Shelby
John McBride -HC/Shelby
Terry Atkins -HC/Shelby
Archie Pittman -HC/Shelby
Jim Pullen -HC/Charlotte
Carl Burrell -Davis & Floyd/Greenwood
5050H172
- - - - - - - - - - - - - - - - --.-
'---CJ> E
.....
C
Q)
::J .._
C
20000
18000
16000
14000
12000
10000
8000
6000
4000
2000
0
8/1
Inner Tier Influent vs. SBR Effluent
Daily COD
10/1
~ Influent
12/1
-8-SBR Effluent
20000
18000
16000
14000
12000
10000
8000
6000
4000
2000
'---CJ> E
.....
C
Q)
::J .._ .._ w
0::: m
(/)
-------------------
"-CJ'
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~
C (I) :::, -C
14,000
12,000
10,000
8,000
6,000
4,000
2,000
0
8/1
Inner Tier Influent vs SBR Effluent
Rolling Weekly Average COD
14,000
12,000
10,000
8,000
6,000
4,000
2,000
'-----------+----------'------+ 0
10/1 12/1 1 /31
Week Beginning
--¼ Influent -8-SBR Effluent
~
"-CJ'
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~
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(I) :::, --w
Ct'.
(I]
(f)
-------------------
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(I) :, ,._
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100000
10000
1000
100
10
Inner Tier Influent vs. SBR Effluent
Doily COD
100000
10000
1000
100
10
1+----------+-----------+----------+1
8/1 10/1 12/1 1/31
-Influent -0-SBR Effluent
'--CJ)
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:::: w
Ct'. m
(f)
-------------------
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'--CJ)
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+-' C
(I)
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100,000
10,000
1,000
100
Inner Tier Influent vs SBR Effluent
Rolling Weekly Average COD
(;)
rfi
100,000
10,000
1,000
100
10-----------t----------+------------+10
8/1 10/1 12/1 1 /31
Week Beginning
--¾--Influent -0-SBR Effluent
~
" CJ)
E
~
+-' C
(I) ::, --w
Cl'.'. m
Cf)
-------------------
--..._
0,
E
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(l)
:J
(.'.)
0
_J
4.50
4.00
3.50
3.00
2.50
1.50
1.00
Inner Tier Influent vs. SBR Effluent
Daily COD
4.50
4.00
3.50
3.00
2.50
2.00
1.50
1.00
0.50+------------+-------------+----------+0.50
8/1 10/1 12/1 1/31
---lf-Influent -0-SBR Effluent
~ C (l)
:J
0 0
_J
-------------------
~ ~
'--CJl
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Q) :, -C
~
<..'.)
0 -'
4.50
4.00
3.50
3.00
2.50
2.00
Inner Tier Influent vs SBR Effluent
Rolling Weekly Average COD
Cl (.')
(.') I;)
& G'.l V G')
el
' I;)
' ~
4.50
4.00
3.50
3.00
(';J
~
2.50
2.00
1 .50 +------------+-----------+-------------+ 1 .50
8/1 10/1 12/1 1/31
Week Beginning
-lt-Influent -0-SBR Effluent
~ ~
'--CJl
E
~ -C
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Ct'. m (/)
~
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0
-'
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Westinghruse Environmental
and Gootechnical Services. Inc.
January 18, 1990
U. s. Environmental Protection Agency
345 Courtland Street, NE
Atlanta, GA 30365
ATTENTION: Ms. Michelle Glenn
Project Manager
•
----~-; ~ ....
4000 ~Kalb Technol;;gy Parkway. NE Suite 250 ·· 4.:: . / / ,.: !':-
Atlanta. Georgia 30340 / . · / ,. ,· · 1 -,
(404) 458-9309 '"""· :~ti,·~-.. 1 •
FAX (404) 458-9438 • IJ
SUBJECT: Request for Sampling Notification variance
Groundwater Treatment System Upset
Operable Unit 1 Remediation
Hoechst Celanese Facility
Shelby, North Carolina
Westinghouse Project 4124-85-050H
Document control Number 85050H-206
Dear Ms. Glenn:
During the past two months there have been sharp periodic increases in COD and ammonia-nitrogen of the the inner tier combined influent and in the same period of time, the SBR has died twice. Hoechst Celanese believes that there may be some connection between the higher strength waste and the problems associated with the SBR operation. During a telephone conversation on January 3, Westinghouse requested EPA approval to waive the 48-hour sampling notification provision in the Consent Decree to have the flexibility to sample as needed when problems with the SBR are apparent. EPA has requested Hoechst Celanese to detail conditions under which the 48-hour notification waiver would take effect. After a review of historical water quality data on the inner tier, Westinghouse feels that the SBR may be impacted when any of three conditions occur: the influent COD exceeds 4000 mg/1, the influent
ammonia-nitrogen exceeds 5 mg/1 or the SBR effluent COD exceeds 400 mg/1. Hoechst Celanese would like to waive the 48-hour notification when any of the above conditions occur. So additional investigate and operational analyses can be performed.
In the event the 48-hour notification is waived and that additional sampling is indicated, EPA will be notified verbally no later than the next business day.
5050H174
A Westmghouse Electric Corporation subsidiary.
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HC/Shelby RD
We_stinghouse Project 4124-85-050H
·15ocumem: Control Number 85050H-206
Page 2
If there are any comments or questions concerning the
information contained in this letter, please contact us
immediately. If we have not heard from you by January 23, 1990,
we will assume that you are in agreement with our request to
waive the sample notification requirement as outlined in this
letter to investigate operational problems within the treatment
system.
Very truly yours,
WESTINGHOUSE ENVIRONMENTAL AND GAATiJJC~V;CES, INC.
M. Kirk Mays, P.E.
Senior Environmental Engineer
(o{;»J}JW ~ ·f-
Everett W. Glover, Jr. P.E.
Project Manager
MKM/EWG/pys
cc: Jim Pullen-He/Charlotte/
Bill Carter-HC/Shelby
John McBride-He/Shelby
Carl Burrell-Davis & Floyd/Grnwd
5050H174
Ron Caldwell-HC/Shelby
Jack Kelley-HC/Shelby
Terry Atkins-HC/Shelby
Ken Mallary-EPA/Atlanta
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. Westinghwse Environmental
and Gllllechnical Services, Inc.
January 18, 1990
u. s. E\ivironmental Protection
345 Courtland Street, NE
Atlanta, GA 30365
Agency
ATTENTION:
SUBJECT:
Ms. Michelle Glenn
Project Manager
Davis & Floyd, Inc. Groundwater
Treatment System -Investigation
Operable Unit 1 Remediation
Hoechst Celanese Facility
4000 DeKalb Technology Parkway. NE
Suite 250
Atlanla. Georgia 30340
,.__ (4041458-9309 J, rf,\X 14041458-9438
·,_ ,I" r,
, .' tr_, .. I
<~-"tvJ.~; I :t
l
Shelby, North Carolina
Westinghouse Project 4124-85-050H
Document Control Number 85050H-208
Dear Ms. Glenn:
During the past two months, the SBR has died twice.
Additionally, prior to the deaths of the SBR there have been
sharp rises in the COD and ammonia-nitrogen. Hoechst Celanese
believes that there may be some connection between the higher
strength waste and the problems associated with the SBR
operation. To ascertain the nature of the problem and possible
solutions, Davis and Floyd, Inc., a wastewater consultant, has
been hired by Hoechst Celanese to conduct a study. Davis and
Floyd Inc. has proposed a study which includes an investigation
of plant operations, an examination of the sources of the high
strength wastes, and a better understanding of the groundwater
treatment operation.
The study proposed by Davis and Floyd, Inc. would examine the
following areas related to the inner tier:
0
0
The influent would be screened with Polytox tests to see if
Phenobac may enhance biomass activity.
The SBR sludge would be analyzed for antimony, chromium,
lead and zinc to examine if bioaccumulation of toxic
materials may be occurring.
5050H175
A Westinghouse Electric Corporauon subs1d1ary.
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HC/Shelby RD
Westinghouse Project 4124-85-0SOH
Documenc Control Number 85050H-208
Page 2
0
0
0
0
0
0
0
0
0
Oxygen uptake tests would be conducted on the SBR mixed
liquor and the individual wells. The information would
address biomass toxicity associated with the SBR and
individual wells.
Micotox bacteria would be used to screen individual wells
and combined flow for toxic materials.
During the project, the biomass would be examined daily to
determine biomass makeup and relative condition of same.
Davis and Floyd, Inc. would examine the benefit in adding a
coagulate to enhance solids separation in the SBR.
The study would examine nutrient loading to the SBR and
current operational practices. Davis and Floyd, Inc. would
determine the proper nutrient level and required
supplemental feeding schedule.
In addition to the current analyses performed, the inner
tier extraction wells would be analyzed for phenols,
sulfate, and antimony.
An indepth evaluation of the groundwater treatment plant
operations, individual well performance and monitoring
system would be part of the study.
The study may include additional operational guidance that
may be added as a supplement to the Project Operations Plan.
Davis and Floyd, Inc. may include a section on operator
training as Hoechst Celanese feels that hands-on operator
training may be required.
The Davis and Floyd, Inc. study would take approximately two
months and would include the additional sampling detailed
above. The proposed sampling frequency, sample points and
parameters to be analyzed are listed in Table 1.
5050H175
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HC/Shelby RD
Westinghouse Project 4124-85-0SOH
Document Control Number 85050H-__
Page 3
Hoechst Celanese would like to proceed immediately with the
testing program outlined in this letter. If there are any
comments or questions concerning the information contained in
this letter, please contact us immediately. If we have not
heard from you by January 24, 1990, we will assume that you are
in agreement the testing program and that we may proceed.
Very truly yours,
WESTINGHOUSE ENVIRONMENTAL AND
GEOTECHNICAL SERVICES, INC.
M lti 'MCUJs
M. Kirk Mays, P.E.
Senior Environmental Engineer
(o~ [D t)kw_{ i
Everett W. Glover, Jr. P.E.
Project Manager
MKM/EWG
cc: Jim Pullen-HC/Charlotte /
Bill Carter-HC/Shelby
John McBride-HC/Shelby
earl Burrell-Davis & Floyd/Grnwd
5050H175
Ron Caldwell-HC/Shelby
Jack Kelley-HC/Shelby
Terry Atkins-HC/Shelby
Ken Mallary-EPA/Atlanta
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Table 1
Proposed Salll)l ing & Frequency
Equal.
Pl ant Tank SBR SBR SBR SBR
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Parameter Frequency Influent Effluent Influent Tank Effluent Sludge ·······------------------------------------------------------------------------------------pH Daily X X X loo Total
,: Soluble
IH3-N
Phosphorous
lotal Kjeldahl
N 1 trogen
litrate Nitrogen
Oxygen Uptake
lettleable Sol ids
Sulfate
lhenols
-ss
ron Total
Iron Soluble
Antimony Total
lntimony Dissolved
lopper Total
opper Dissolved
lhromil.111 Total
Chromil.111 Dissolved
lead Total
Zinc Total
line Dissolved
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Daily
lleekly
2/week
2/week
2/week
2/week
2/week
Daily
Daily
2/week
2/week
Daily
2/week
2/week
2/weelc
2/week
2/week
2/week
2/week
2/week
2/weelc
2/weelc
2/week
X
X
X
X
X
X
X
X
X
X
X
X
X X
X
X
X
X
X X
X X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
X
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114
TRANSMITTAL
MEMORANDUM ___J,_
Date:
-~ /"'"='Ft .... r➔1;1r,-: :.7t." :'"-,-,,.. ~•,, ~
Attention: ///,-_.
Client:
Cl Westinghouse Environmental
and Geotechnical Services, Inc.
Suite 250
4000 DeKalb Technology Parkway. NE
Atlanta, Georgia 30340
(404) 458-9309
FAX (404) 458-9438
Per Your Request
For Your Information/Records
For Your Approval/Comments
For Your Review/Comments
Revise and Resubmit
Approved as Noted
Resubmit for Record Copy
Approved
Please Return
Returning to You
For Correction
Not Approved
' ..,_ ,,.. ,. , , , ' , I I' t,,! , .. ,._
-. .{ r, i i.nL
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1t4
• •
TRANSMITTAL
MEMORANDUM J_
Date: :)1-z./,;.o
f..:.TLAN \H (-r,1...--.-I.. ':;.Cc, S
Attention: I,. I\ , 1 •~-, ·..: ,:..,_....,J;10::. ~Ir,.\ lo.v• -.J
Job No.: -1-,2.,:;. _ ?"' _ c,s-r,,-/
Client:
/Jin~. Jc·
f;;aeE-rr lu-0Lovert-,J.·.
C ·C. l'_.,,_ (',,__(} dwell µ' / 5 /.,__( l,
/1 j
{_(!(_( t ... .w_ J._~~·m., I.-...... !ff' I" •(, I ~ c> ..,_, ,
(i0,U' ... Cr-.,, T.r-<-H( /!>t,,,_( 1.,. /
/'-' ✓-
1 __ ,r, c!:... 1(,(_(_..,.)--/>~j.~J...( i,,_
V I
__ ,, (,.__," 1-( c.12, t.Ac!, , '-'<-/'-;.,_f._ 1., ",r
\
\
e Westinghouse Environmental
and Geotechnical Services. Inc.
Suite 250
4000 DeKalb Technology Parkway. NE
Atlanta. Georgia 30340
(404) 458-9309
FAX (404) 458-9438
Per Your Request
For Your Information/Records
For Your Approval/Comments
For Your Review/Comments
Revise and Resubmit
Approved as Noted
Resubmit for Record Copy
Approved
Please Return
Returning to You
For Correction
Not Approved
,_ (./,,_ ( &,,
1
/.IC / (_j.c,J..,., 1-k.
,~>--M, ,~~rlt.,.1.,,i 1-tc.//:,,_,~•·'~'--1'-r,f; ....
,.::..~ i_-:,, .._,1___U
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PHENOBAC1
Microbial Hydrocarbon Degrader
FOR USE IN: Indll!lri:il 1111d mo.aufacruru:a hydro=boa waste di.scllaraas. sucb. lS, che111!co.l placta, steel mllla, !utile :u,.d rood proceSSU1g pla.acs.
ngscgrmoN PHENOBAC hu beH aciectifiC3lly designed tor biO-allplCDIAd011 oC laduattlal and m11111faetur1D1 hydroc:arboa waste diaclw-ses, For pretreatment or complete oa•slto trc111.meac ayacems and la mllllicipal syiccma recolviq mixed w11&1ewatcr from coiamualcy actlvicles, Wuce, with a attons or1anlc co1111nt aad blah .BOD may coo.cu various level, of blologlcally roalataiit quasl,toxlc and 10JCio compoUDda chat Interfere with 110rmd opefflt!o11 of w:iatfl'N:ltor ttontmea.c ayscema, Blomua ollhucemD'III widl PIIENOBAC wW enable tbe tteo.tmeiu aystcm to etticieatly, ll!ld o!ectively deande organic wucea that coo.WA complex orp.aica, aucb u phenols. beazeaea, allphedc and aromatic hydrocarbons, me1~cryl11e1, ultrllcs, Gl'cosols. aapthaleau, IIIIWIN, organic alcoboll, ayntbotlc detcr~n!$ and 1W'f11clllllt1, go.aollna, kerosene, Nol 111d machlao olla, formaldehyde. iij'COla, hc1erocycio1 auch as morpbollno and pyridlna, ctboxylated phenols, WllXCS 111d ocher difficult 10 crut compo1111da. Cy:lllides 11e biologlcolly removod Crom 1oh1tio11.
P'OR.M
COLOR
SPl:?CtFIC GRAVITY
CONTENI'S
STORAGI!
CAUTION
PRQQYCI SP!CJFJCA'flQN!i
: Frce-Howill1 powder
: Bull to brown : o.s · 0,7
: Adapted micrcx,,g:iaiuu1 111d ~II atlmulADIS
: Store 1caJcd In a dry Alta 11 rOQffl temperature not excoodlng 100 dogroe1 P. DO NOT AI.LOW PRODUCT TO Bl!COMB WET OR MO(ST PJUOR TO USE, DO NOT FRE!E!ZE.
: loh41atlon o.nd direct skin contact should be avoided. 01111 protocdon for eyes, 11osc and mouth should be used. In the ovcat of direct concoct with the 1kia or eye,, Dush the affected o.rea with wncer, If irri111tio11 pel'liill, coo.tact your ph)11Ci4D.
Q(BECTJQNS fQB USE 1. Ol&pcm 01111 !)Mt PHENOBAC la elibt 10 ten parta W1ll1D wutor, (about 0110 po1111d per 1allo11), The water sbould be about 80 • 100 degrca P. Allow to 11111d tor C•IIC to two houri with ocwlolllll lti.rriq. 2. Do oot add PHENOBAC to enc cre11tme11t system 11 a locatlo11 where 10,cic or otherwiH adveno pH, dlssolvcd oicyae11 or te111per11turc co11dltlo111 m11y exist Al peak lovcla, 3, Applica.tioa R11tes: Co111ult your Polybac rcprcsc11tatlva prior to uso tor specific lnstructlo11,. ~-For appllc:i.tlo11 011 ,pill&, u~ your Polybac ropresentotivc for o deuliled appllcotion prorram, $. In mil.lly cnae1, PHENOBAC ls best 11aod In combination witb oi;hor 11utricn11, aolvcnt 5)1toma or other biocbeadcnl&. Camult your Polyb3c repreaeat3tivo with your req11irem$ntl,
For optima.I rosult& the w.a.stcwatcr tru1mco1 system should meet tho rollowlng condition,:
lnlluont pH
Oluolvcd oxygon, ppm
C/N/P rnda
Tempcr11turc, C degrees
Toxic metal&. pp~
( e.g., bCJC., cluoqiium)
OPTIMUM
7.0
2.0 ..
100/10/1
30(861')
0
MINIMUM
6.0
1.0
100/S/l
10(,0 I')
0
MA.XlMUM
9.0 ...
100/20/1
40(104 F)
2
•\, __________ _
·,,, ,,
...... · .. -, ~1 '8 .. :rm ---L ,.u c1r-1 ..,J -~ ·t. ·C C 1--1 ,' ,_, /,-. . 7
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4YAIY:DJLO:X 25 lb. plutlc pails &Ad 100 lb. polyethylcnc•IIDed &bar clruma.
BJQMA.V JNQJNEEBJNO Bio111w Engineerilla; by Polybac Corporuioa Cllll ao1vc )'QIII' bloloaical waste ueatmen1 problew. Biomass Eopnccriilg combl.aea btocliemical procesa eqwerlng with the uu of ,poeialized adapted mkroorglJllsma. TbJa ia a powGrill1 combi.aation for i,olvlng oporaling problem ill blological waste~or ttolltlDOU plama IIAd blologiC41 waste dlaposal sys1ema. Cllll on Pol)1)ac: tor a detailed Blo111w Enginoorlng propow for your plant. '
QISCL\JMJB Thia io!ol"l!lation is representative only 111d 'dlere are 110 warr1111tlc:1 of pcrforma11co, expreued or lmpllod. Polybac Corporlllion baa ao control over 11orap, lwidllng. or product applic:itloii oocdldoaa, thorofore, Polybac Corporatloii alaall 110t be liable for. da,uget ot 111y kilid uwn1 fro111 th• prcaonco or IIIO ot !he prodllW deceribod. '
PHENOBAC LI maau!acturcd and dlstribu1ed oxcluslvely by Polybac Corpor1tio11, ~S4 Ma.rcon Blvd., AlleotOWII, PA. 18103. U,SA 1988.
•Material Safety Data Sheet
Mil;Y be' usild ta oamply with OSHA's Hazard CcmmunlClllon 814ndard, a29_~FR'1ij10.1200. Sllndll'CI muet bt r:1,:utttd for speclftc requtremenll.
U.S. Depairtment of Labor
Occup1tlon1I Saluy and Ht1lll1 Admlnlstmlon
(Non-Malldatorv Form)
Form Appro'ltd
OMB No. 1:na-0012
N«tl Sllnk ,,._ .,,, nor ptlffl/ll9d. I/ IIIY "9111 ii ,,,,,, _.._ /Ir flO /nlamNIIIO,, Ir 1vf/l&Olt. rhe 1pac. muat t>f mlnl9d lo fnlllOI» C'llr.
Man11lecw,er'1 N11M
~llAC C0IPOU'l'I0lf
~• (Numbtlr. ._ C#J,, ltlll. -ltl' OOdt) 3894 Coqrtue• Street
lech.lehea, PA 18017
Eton 11 -Hazardous lngrcdlent&lldentlty lntormauon
l!mar;er!O'f Ttlt~ Numblt
(215) 1367-733R
Teiepnont NUll1IW for tnfOrmlllOn
C2U' 1!67-7338
er MW1d0Ue ~ (8pe,;diO Cl,tmlcll '4tnllty; Common Name(II)) 05HA PIL ACGIH nv llecommtnatcl ~ bazardou1. Misad b:lochemj,call, euzm,1 aod 111rfac:tauts ou bran baH,
t----~--------
1=--------------------~----------------------------------1-----------------
t= ___________ _
Eon Ill -Physfcal/Chemlcal Characte11stlca
Bollin, Pcint
f Pt-19 (mm H9,)
v.ji;' Ounslty (Al~ • 1)
ufu,i Fl,. ano l!,cpjoslOII ~
H A
co Bt'OWll Color; llu1ty Odor.
0.5 -0.7
IA
NA
I Se<:llon V -Reae1lvlty Data
~lal:<11\Y • l Unstallle !
, , SWIii
I 1 i;;ec,mcallblllty (Mlltr/1/a to A~old)
Water
D01'0Tffl.
Ha.tan:10"1 Decgml)Ofil!Otl or S~ 1 ::=:... 1:: .... I ,f .. -·-
1 SG¢t!On VI -Health Hazard Dita
AO<Jte(I) of int,y: w;ii\Gif 81dn? I Duat .A.void direct sld.sl contact. Hoellh H&Uldl ,_ MO Chronic) _ Vash bands with 1oap and water after ba'lltlU.ngs noid d1rtct •kin 1.sooucc; all■rgic
ea;::u,ogonlotly.
s•actiou co euzp1 111,ay oceur 111 hYpenaa,ttiva illlliv1dua11.
I NIA
NTP? IARC MorioQrapnl?
'tl/1 I/A
sl;,,s and Symptoma al ~,.
I It
Mec!lcal CcnclltlOnl
I ~ltrwlly "WnlYlltcl 11v &,pccl\ll9 ladividuals llypsrallargic to ea.zymea or other related prote1A1
_ 9hould a~ t,•n41t, emergency &nCI P1'00IOUNls
Wash hai!sJ• With tW and vater after handl:l.ng; a-,otd diraet skin l..5.Sata9t or tahalatiop; a1lorgic raacti99• may oocyr in h:z:perseaait1vc 1ad1v1d9l1, Section VII -Pl'ICluttona for Saft Handling and Use
I StePS tD e, Tlkllll In Cua Mltlltll " rn 0, ·~Ued
SHISP yp/coll~ct1 avoid direct rkta copcact w/or --~ii:-oc;t inhalation of dupe.
I Wastt Ol"f'OSII MtthOd No fpaeial dispgaal method. May ba sewered; compatible vitb all lcnolm I t,iologic;al tr■acmept ■atbods,
Procautlont to St T8kon ill Handling Ind eioiine
I c:011tacc, ~ .. , Preca\1110n1
Llep dry ud •t ro0111 ce111paratura. Avoid direct ak111
pq pot :t.pgast. Avoid dupt 1 Wa11h bands •rith soap and veter af'ter use, I l'p,Uviduals hYpJ[IS9f1tiva
11;:;ctlon VIII -Control Measures
AO';plralOt'f l'rocec,IQn ™ $.) I Tes,
Fe;,ulatlon LOOal !Niau• ta■
to e0~3•• or o~hei:-Tllat,ad prota1ua 1hould 11ot bandle.
eur,rical uak or eciuiva'laut.
s~;a1
O!lltt·
I !)'t Protto1l0rt
f•s (Dust) Ott,., Protect,,,. Clolft1r19 or &quopment I Dyftiug protection •. ~-ri</~yg1en1c P'ract•~•
Do got ipge1t. Avoid dust. Wash baad.:J with so~p and water afteT lllle.
••
Po99 2
1 3 : 1 1
FEE:-1. 5 -·=·· .. :1
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nEPA f
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THU B 19 POLYBAC CORP
Unitttd Statn
en"ironm4ntal Proitetio"
AQoney
Wa1■r
Office ot
wnor
WHhin9ton OC 20-460
F'A
E?A 4-10/4-87-00S
July 1987
Permit Writer':s Guide
to Water Quality-Based
Permitting for
Toxic Pollutan1ts *
~he National Discharge Eliminatinn System (~?DES)
per~its program has begun to focu~ regulatory
attention on the control of toxic i>ollutancs to
protect water quality. Recent pesHaqe of amendments
to the Clean Water Act and the acqu!s!tion o!
increasing amounts c~ data on the ccxicit~ of
eff~uent~ point to the need for an increased
effort to control the d~scharge of toxic µcllutants.
The P~rrrd:. Wri1:cer',c1 (;l.lidCJ to Wqt.:!1 ~)iAUZi,::-1-aa,-.:ed
P~rmitting fer Toxic PoZlwtantc• provides procedural
recommendations to State and feder<1l NPDI::S permit
writers on setting water quality-based permit limits
for toxic pollutants.
In Appendix c of the guide, the ''Overview of Selu~tea
Available Tools", summarizes sCJmc of the important
tools that can be used to identify and control
unacceptable toxicity in effluents. On page C-3,
listed under MICROBIAL TESTS, POl,Y'l'OX is recommended
as an inexpensive tool to test foe effluent toxicity.
"EPA document 440/4-87-005, July 1987
----------------------
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1=-E.B-15-·:=,~-::::1 THU 8 18 POLYBAC CORP PA
rl POL YfOX"' RA.PIO TOXICITY TEST
TECHNICAL DATA SHEET
FOR USE IN: Determining the biological dccomposibility or to,icity of wastewater lo a sewage lrealmenl
facility,
DESCRIPTION
POL'\TOX,. i.~ a blend of spcciali1.cd microbial cultures in an casy•to•use kit, designed to provide a simple,
rapid test for measuring the lmcicity of wastewater to biological systems within 30 minutes, without the use of
expensive instrumentation.
WASTEWATER TREATMENT
The biological processing of organic waste in industrial and municipal wastewater streams has long been
known. Although these biological systems typically utili,c microorganisms to effect biochemical
decomposition of the organic waste, the processing plants may differ in the method in which the
microorganisms are cultivated,
A variety of test procedures have been developed for analyzing the quality of sewage for the purpose of
determining the toxicity of the sewage, for determining the total chemical content in the sewage, and to
determine the oxygen consumption rate of the microorganisms in that sewage. By and large, many of the
analytical processes have involved the utilization of complicated equipment or have taken a substantial
amount of time in order to complete the test and obtain meaningful results. In many cases, because of the
time required to obtain the results, sufficient time has not been provided to take the appropriate action with
respect to the handling of the incoming sewage, As a result, the plants in many cases were rendered inactive.
AD auractive solution to analY7.ing the quality of waslcwalcr is POL'\TOX,.. This process is capable of
providing analytical information with respect to the toxicity of iricoming sewage to a waste disposal plant, the
quantity of organic component in the sewage, and the type of t,,xic matcrfal in a particular system in a quick
and efficient manner.
By utilization of a specially prepared bacterial culture and thi,ough the measurement of dissolved oxygen
content, one can achieve ,several advantages which have not b,:cn available with prior processes. Some of
these advantages include:
-· a rapid biological lest for determining the loxicily of a wastewater sample
•· a rapid, reproducible, biological test for determining the toxicity of a specific chemical as a
function of its concentration in water
•· a rapid method of assessing the toxicity of acidic or basic solutions of various specific chemicals,
and organic and inorganic compounds lo bacteria
·· a rapid method of determining the effects of pH, temperature or a dissolved gas such as H2S,
NH3 and CO2 on bacteria
PQLYJQX,.:
•· Is EPA recommended
•· Is free of nitrifying microorganisms
·· Has been marketed since 1984
•· Is sold around the World
•· ls always a stocked item
•· Orders are shipped within 24 hours of receipt
-· Is backed by a money-back guarantee or rcplaccmcnl if nm completely satisfied
COURTNEY Pl.ACE.:• J8,4.COURTNEY STREET• BETHI.EHEM. t•A 18017-89,9 • {21S) 867•7:lJB
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THU POL"•,·"E:AC CO F: P F"A
SPECIFICATIONS
: Buff to brown. Form
Contents
Container
Storage
Expiration
Caution
: 8.0 grams of a specialized blend of microbial cultures, grOWth stimulants and pl I buffer.
: Polyfoam mailer containing 20 clear, polystyrene vials.
: Store scaled in dry place al room temperature (20'C), Do not free1,e or refrigerate.
: For best results, use within three lo six months of receipt.
: Avoid inhalation and contact with eyes, skin, and clothing. Wash thoroughly after handling. In
case of contact, immediately flush eyes with plenty of water for at least 15 minutes. Call •
phy.;ician. Flush skin with water. Wash clothing befmr: reuse.
DJRECTIONS FOR US€
POLYTOX"' is a granular, free-flowing, dehydrated powder which must be rehydrated bdurc use. Du not
allow POLYTOX"' to become wet or moist before use. Always store in a dry area, preferably at room
temperature (20•q. Do not store in a chemical cabinet.
IMPORTANT:
For best results, toxicity tests should meet the following conditions:
Sample Pretreatment Parameters
pH
Dissolved oxygen, ppm
Temperature,' C
Toxic metals, ppm (e.g., hex. chronium)
PACKAGING
20 vials/unit
OTHER LABORATORY PRODUCTS
function
Optimum
7,00
8.00
20
0
BOD Seed Inoculum
~~
6.50
7.00
18
0
~
POLYSEEo@
POL YSEED-NX®
NITROSEED-ST"'
CBOD Seed Inoculum -Inhibits Nitrification
Toxicity Test for Nitrifying Microorganisms
BIOMASS ENGINEERING®
Maximum
7.50
9.00
22
1
Biomass Engineering by Polybac Corporation can solve your biotogiicat waste treatment problems, as well as
your analytical test problems. Biomass Engineering combines biochemical process engineering with the use
of specialized microorganisms. This dual approach offers enhanced prohlem solving capahilities to biological
wastewater treatment plants, remedial spill clean-up sites, and analytical testing laboratories. Polybac's
personnel are trained and experienced. Call on Polybac's pcrsor. nel fm a detailed Biomass Engineering
proposal for your plant or to enhance your analytical testing.
»ISCL4.IMER
The information presented in the Technical Dara Sheet is believed lO he accurate and reliable. This
information is presented as representative only and there arc no warranties of performance, express or
implied. Since Polybac Corporation has no control over storage, handling or application conditions, Polybac
Corporation shall not be liable for damages of any kind arising from the presence or use of the products
described.
POLYTOX"' is manufactured exclusively hy Polyhac Corporati,m, Courtney Place, 3894 Courtney St.,
Bethlehem, PA 1R017-R99Q USA. 1990.
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Yj APPLICATION. PROCEDURE
POLITOX" RAPID TOXICITY ~rEST PROCEDURE
BACKGROUND
ln 19n, the United States .Clean Water Act declared a need to regulate the discharge of toxic pol-
lutants into the nation's water supply. This Act allowed the Envirotw1eotal Protection Asency (EPA), through
the use of permits, to establish rules and regulations governing the 'pretreatment• of industrial wastewaten
prior lo their discharge.
An attractive solution to analyzing the quality of wastewater is POL VTOX. POL Yl'OX pra--idcs a
simple, rapid test for measuring the toxicity or wastewater lo biological wastewater treatme11t systelll5.
POLYfOX contains spcciali7.ed microbial cultures and can determine the toxicity of wastewater& and chemi<:al&
in biological treatment S)'$tems in 30 minutes, with no expensive instrumentation required.
The process described in this 'Applicatioo Procedure' evaluates the inhibitory effect of the W85tewaler
or chemical(s) to the spccialiud bacterial cultures by measuring the respiration rate under defined conditiona
in the presence of different concentrations of that wastewater or chemical The respiration rate is the oxygen
consumed by the aerobic bacterial cultures and is exprcsaed in mg o2 per liter per minute.
POLYfOX is designed to provide a rapid screening method whereby wastewaters and chemicals which
may adversely affect the biomass of a wastewater treatment facility can be determined, and non-inhibitory con•
ccntrations for wastewaters and chemicals prescribed. This test kit Is most applicable to wastewatera and
chemicals that are likely to remain in solution. The Lethal Concentration, 1.e:30, in this procedure is the con-
centratioo of the wastewater or chemical at which the respiration rate is 30% or that exhibited by the baseline
or control The inhibitory or toxk effect of the wastewater or chemical at a specific concentration is expressed
as a percent or the baseline respiration rate. A testing procedure utilizing at least 5 different concentrations is
recommended.
The 1--<;o value should be regarded merely as a guideline of toxicity for that particular wastewater or
chemical to its own wai;tewatcr microorganisms, sina: the naturally occurring environment C8Dl!Ot be duplicated
exactly under laboratory conditions.
EQUIPMENT REQUIRED
• Staodard (300 ml) BOD bollle(s) (e.g., Wheaton '800" brand glass).
• Dissolved oxygen probe and meter. The prolle must fit snugly into •the neck of the BOD bottle, elimioating
all head space.
· One-inch magnetic stirring bar aud magnetic stirrer or self-stirring dissolved oxygen probe capable of
suspending the l'OLITOX populations in the BOD bonle (e.g., the YSI seir-stirring BOD probe).
• Aeration devia: (e.g., aquarium pump, tubing and air stone).
• One and two liter containers lo be used for the aeration of the distilled or deionized water (control)
and wastewater or chemical (test) samples.
• pH adjusting solution (e.g., dilute sodium hydroxide or sulfuric acid).
• Thermometer.
· Funnel.
• Stopwatch.
-(Optional) A single channel recorder connected lo the dissolved oxygen meter to provide a continuous
strip chart recording of the dissolved o,-ygcn level in the BOD bottle v,:rsus time.
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LEST CONDITIONS
. ~oration/contact time: 19 and 21 minutes
-O>ntaincrs: 1 liter &ize for the aeration or the control(s), 2 liter size for the aeration of the tcst(s)
. Air Supply: clean, oil-frco air
. Water: Deionized and/or distilled water
• Reactor Vessel: BOD boule(s)
-Test Solution: The freshly prepared wastewater or cbemiciJ solution (e.g., aerated solution with pH and
temperature adjusted)
-Control: Baseline respiration rate for the POL YTOX populations only
-Temperature: 20 :I: 2 • C.
PROCEDURE FOR BASELINE ACTIVITY
SW2:
L Calibrate the dissolved oxygen probe and meter according to the manufacturer's specifications.
2. Air &aturatc 500 mil of pH adjusted (7.0) dcioo.iud or distilled w.,tcr by aerating the water for at least 30 ·
minutes at a relatively constant temperature (20 ± 2' C),
3. Pour 50 mis of the aerated, pH adjusted water into a small beaker and sci side.
4. Remove the cap from one of the POLYTOX vials. Place a funnel into the ncclc of a clean, dry BOD bottle.
Pour the dry bacterial contents of the 'lial into the BOD bottle.
5. Add the magnetic stirring bar lo the BOD bottle if a self-stirring probe is not available.
6. With stopwatch in hand, pour the premca•ured 50 mis of water into the BOD bottle containing the
POLYI"OX vial contents. IMMEDIATELY START THE STOPWATCH.
7. Piclc up the BOD bottle and swirl the contents for 25 to 30 seconds, making sure that the POLYrOX
populations are thoroughly wet and thus acdvatcd.
8. Hold tho BOD bottle at a 45 • angle and pour additional pre-aerated water into the BOD bottle. Pour the
water down the side of the bottle to avoid tbe formation of excess nir bubbles. Fill the bottle to a level just
above the bottom of the ground glass joint.
9. Place the bottle on a flat surface and tap gently to remove bubbles.
10. Insert the dissolved oxygen probe into the BOD bottle, carefully displacing all bubbles from the bottle. It
helps to tilt the bottle to the side so that bubbles will slide off the face of the dissolved oxygen probe
membrane.
11. Initiate stirring in the BOD bottle.
12. The dissolved oxygen level should be at least 6.5 mg/I at this tim,,. Record the· dissolved oxygen reading
continuously with the optional recorder or every two minutes by hand. After you are familiar with the
procedure, the dissolved oxygen level can be recorded at the pe,ctinent times of 19 and 21 minutes only.
NOTE: With practice, the disoolvcd oxygen probe can be plaa:d in the bottle within 60 to 90 aeconds
after adding the first 50 mls of pre-aerated water.
13. Use the following equation to c.alculate the dissolved oxygen uptake rate for the baseline acti-;ity of the
POL YTOX populations:
Equ•tion 1:
0019s . 00215
DOURs ~ --------= mg/I/min
2 mins.
DOURs ~ Baseline Dissolved Oxygen Uptake Rate
D019S = Dissolved Oxygen ( mg/I) al 19 minutes
D021S = Dissolved Oxygen (mg/I) al 21 minutes
P_n7
I Fo~ atiY POLYTOX kit, the ba.elioe rate of respiration ~ deionized ~r distilled water should range between
, · O.~ to 0.40 mg/I/min. The baseline respiration rate for your POLITOX kit should remain with.i.a this range
I for at least three months if the kit is stored at 20 :t 5• C. (DO NOT FREEZE OR REFRIGERATE.) A
baseline ,hould be run for each series of tests.
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PROCEDUREFORBACKGROUNDACTIVITYOFSAMPLE
To acrollllt for any rackgroWld oxygen depletion caused by either miaobes present in the sample itself or by
the stripping away of COD (Chemical Oxygen Demand) during aeration, the samplc(s) must also be tested in
the absence of the POL ITOX populatio11S.
~=
1. Calibrate the dissolved oqgen probe and meter according lo the manufacturer's specification.
2. Air ulllfate one liter of wastewater or test solution (full strcogtl:.) by aerating the sample for at least 30
minlltc$ at a relatively constant temperature (20 :I: 2' C).
3. U ne~, adjust the pH of the wa.tewater or solution to 7.0 with dilute sodium hydroxide or sulfutic acid.
4. Add the m11gJ1etic stirring bar to the BOD bottle if a self-st.irrin& probe is not available.
5. Hold the BOD bottle ata 45' angle and pout pre-aerated wlutio11 into the BOD bottle. Pour the wnplc
down the side of the bottle to avoid the formation of excess air bubbles. Fill the bottle to a !eve I just abowe
the bottom of the ground glass joint.
6. Place the bottle on a fiat surface and tap gently lo remove bubbles.
7. Insert the dissolved oxygen probe into the BOD bottle, carefully ciisplacing all bubbles from the bottle. It
helps to tilt the bottle to the side so that bubbles will slide off the face of the dissolved oxygen probe
membr&®.
8. Initiate stirring in the BOD bottle.
9. The dissolved oxygen level should be at least 8.0 mg/I at this time. Record !he dissolved oxygen reading
continuously with the optional recorder or every two minutes by hand. After you are familiar with the
proo:dure, the dissolved oxygen level can be recorded at the pc,rtinent times of 19 and 21 minutes only.
NOTE: If the dissolved oxygen is less than 8.0 ing/~ the 30 minute prcaeratio11 procedure must be repeated
and the dissolved oxygen level rechecked. U it is still less than 8.0 mg/~ ii is likely that a significant
chemical 07')'gen demand exists in the test solution. This will interfere with the POLYTOX test and
must be eliminated. Overnight aeration of the sample may be sufficient to remove the immediate
oxygen demand. It should also be noted that removal of the chemical oxygen demand by air
stripping methods could change the levels of inhibition exhibited by the sample.
10. Use the following equation to calculate the dissolved oxygen uptake rate for the background activity of the
=pie:
Equation 2:
DOURB • --------• mg/1/miu
2mw.s.
DOUR8 • Background Dissolved Oxygen Uptake Rate
DO19B • Dissolved Oxygen (mg/I) at 19 minutes
DO21 8 ~ Dissolved Oxygen (mi;/1) at 21 minutes
For any given sample, the background rate of rcspiratio11 should be less than 0.05 mg/I/min.
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PROCEDURE FOR TOXICITY TEST.
With ·the dissol:.Cd oxygen probe and meter calibrated according to the manu!ac~er's specifications and the
tc,~ sample pre-aerated, pH and temperature adjusted, proceed onto the following steps:
~:
l. Pour 50 mls of the sample into a small beaker and set aside.
2. Remove the cap from one of the POL YfOX vials. Place a fwwel into the neck of a clean, dry BOD
bottle. Pour the dry bacterial contents into the BOD bottle.
3. Add the magnetic stirring bar to the BGD bottle if a self-stirring probe is not available.
4. With stopwatch in hand, pour the premeasurcd 50 mis of sample into the BOD bottle containing the
POLYfOX vial contents. IMMEDIATELY START THE STOPWA1'CH.
5. Pick up the BOD bottle and swirl the contents for 2S to 30 seconds, making sure tbat the POLYI'OX
populations are thoroughly wet and thus activated.
6. Hold the BOD bottle at a 45 • angle and pour additional pre-aerated solution into the BOD bottle. Pour the
sample down the side of the bottle to avoid the formation of cxa:ss air bubbles. Fill the bottle to a level
jtt<t above the bottom of the ground glass joint.
7. Place the bottle on a flat surface and tap gently to remove bubbles.
8. Insert the dissolved Oll}'l!en probe into the BOD bottle, carefully displacing all bubbles from the bottle. It
helps to tilt the bottle to the side so that bubbles will slide off the face of the dissolved oxygen probe
membrane.
9. Initiate stining in the BOD bottle.
10. The dissolved oxygen level should be at least 6.5 mg/I at this time. Record the dissolved oxygen reading
continuo\1$ly with the optional recorder or every two minutes by hand. After you arc familiar with the
procedure, the dissolved Oll}'l!en level can be recorded at tbc pertinent times of 19 and 21 minutes only.
11. Use the following equation to calculate the dissolved oxygen uptake rate for the test sample:
Equation 3:
D0191" • D021r
DOlffir • -------= mg/I/min
2mins.
DOURr ~ Dissolved Oxygen Uptake Rate for the Test Solution
DO19r " Dissolved Oxygen (mg/I) at 19 minutes
DO21T • Dissolved Oxygen (mg/I) at 21 minutes
12. Use the following equation to calculate the corrected dissolved oxygen uptake rate for the sample to account
for any background activity (DOUR8 ).
Equation 4:
DOURC • Corrected Dissolved Oxygen Uptake Rate for the Tes,: Solution
DOURT • Dissolved Oxygen Uptake Rate for the Test Solution
DOUR8 n Background Dissolved Oxygen Uptake Rate ·
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If ,:he respiration of the test solution is lower than the baseline rate, then the lest solution is con.sidered in-
b.ibi1ery to the microorganism&.
13. Use the following equation to C3lculate the perceut whibitioo of the test s=ple to the POLYTOX
populations:
Equati011 S:
DOURc
1 • ------X 100 • % INHIBmON
DOURg
DOURc • Corrected Dissolved Oxygen UpLlke Rate
DOURg • BasdiJle Dissolved Oxygen Uptake Rate
If the inhibition is sismfic:mt, it may be desirable to dilute the test wastewater or c;hemical aolution and repeat
the POLYl'OX test procedure. Testing at various dilution.s can be used to determine the concentration at which
30% inhibition of the inicroorganisms occurs, LC:Jo-(For the purposc.1 of the POLYTOX toxicity testing proce-
dure, inhibition of microorganisms is equated to reduction io dissolved oitygcn utilization by the
microorganisllls). For example, the following charts provide the LC:3o for pure phenol based on data ge11c~atcd
using the POL YI'OX procedure. For additional information, sec "Application Notes'.
DETERMINING LC30 FOR PHENOL
USING POLYTOX DATA
"'
t, • 11,1,c1-,1 ' .__~...._. ............... __....___._....._._........,
,.. lH4 11 ...
~~no,t ...
s.
.!! -:: -----p 0 " M Q
10
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RAPID TOXICITY TEST
,mn AT m,aas mmmna11
At!SiR1 'C! a.r,J
l11 tltl
' '-....,-,--"'-,--..,,-,.,--,~'--,,,,.,--
Tlll lllHIUi
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APPLICATION N01'ES
NOTE#DOBE8
When the dissolved oxygen level is at least 8.0 mg/~ but the background rate o[ respiration (DOUR8)
is greater than 0.05 mg/I/mill.
A DOURa·that is greater than 0.05 mg/I/min. is likely to be a combination of biological and chemical
oxygen demand (i.c~ sample composition and microscopic elWl!ination could verify biological activity).
NOTE#DOBL8
When the background rate of respiration (DOURa) is not zero at full strength and several test solu•
tions (DOURT) are run.
Use a percent of the DOURB that was observed at full stre11gth and subtract that number from the
DOUR-r for each concentration tested.
NOTE#E8
When the dissolved oxygen has been completely depleted prior to the 19 minute reading.
Record the pertinent times in which a dissolved oxygen of at least 1.0 mg/! remains for the baseline
(DOURs) and tests (DOUR-r), Using those times (i.e., 15 and 17 minute readings) calculate the various ac-
tivity rates.
NOJE#TBRE8
When the dissolved oxygen rate of the test (DOURT) is greater than the baseline rate of respiration
(DOURs),
Depending upon the homogeneity of the contaniinants with.in the sample, areas of low organic levels
may even experience 60me enhance111cnt of biological oxygen uptake activity. At concentration of samples
where there is no toxic effect upon the microbial oxygen uptake rate, uptake rates greater than the baseline rate
(DOURs) arc sometimes experienced. These enhanced rates are rcp,·esented as negative inhibition values and
usually indicate the presence of certain readily degradable compounds which the POLYfOX bacteria can im,
mediately utilize as a good source, thereby increasing cellular metabolic activily and the uptake of dissolved
oxygen.
NOTE#SSE8
When the sa111ple is soil or sand.
Using the combined mixing capabilities provided by a self-stirring dis.solved oxygen probe and a 111ag-
octic stir bar, 100 g soil or sand per liter deionized water is the highesl conccotratioo able to be kept thoroughly
mixed. Therefore, it is suggested that the sample be tested at concc11trations of 100.00, 33.33, 3.33, and 0.33 g
sample/I achieved by the addition of30.00, 10.00, 1.00 and 0.1 g samplc/300 ml BOD bottle respectively. Some-
times the limited factor for increasing the concentration of the sample is not the toxicity of the sample to the
microorganisms but the ability to sustain adequate mixing. Upon completing the procedure for the baseline
(DOURs), the background activity (DOURa) o[ the sample mu:;t be checked by the addition of 30.0 g
samplc/300 ml BOD bottle.
By testing different concentrations o[ the sample using these suggested notes, ... Jxicity profile of the
;ample toward the microorganisms can be generated. This infonnation can then be used to determine a
threshold level (maximum threshold) at which lhe waste can be effectively treated.
C:• 1 1
IFEB-15-90 THU 8 : 2 4-
I Material Safety Data Sheet
May be used to comply with
(")~W.d1111 1-11'1.II!"-~ ~--"""'-,..1.1111i~,i•i~.-i tt~l'll~II\~.
29 CFR 1910,1200. Standard must be
CORP PA
U.S. Department of Labor
Occupational Safety and Health Administration
~t-lQn-Manda~•=-ry rgrTfl)
Form Appro1•ed
OM!! Nv, 1218-M72
F' • 1 1
I mns11ttflf1 lnr ~flfli:ifir r~11ir11mRnr1
IDENTITY (As um on Label and use POL YTOX
Ra id Bio1o ical Toxic1t Test Noro: Blank spaco.s aro nor permirred. N any lt'1m Is nor opplicable, Of no
Jnlormatloi1 Is available, rlls space must be mwked to lnd/ca!8 that. I Section I
Manufacturer's Name Emergency Telephone Number
Pnlvbac Cornoration (800\523--9385
I AQdross /Numbe~ Stroot, City, Stato, ono ZIP Cods) Telephone Numl,er for Information
__ co_u_r_t_ne-=y-~_1 a_c_e ___________ -+..,,...'~' ?o:11'.:t ~·.J.J ,, M:m.i,7.:.-J..::,.,. 7 ~, ~)A_ii ___________ _
Ome Prepared
I 3894 Courtney Street
Bethlehem. PA 18017-8999
I Section II -Hazardous Ingredients/Identity Information
Hazardous Components (Spe<:ific Chemical Identity; Common Name(s))
January '1990
Signature ol Pr11parer /opl/onaQ
OSHA PEL ACGIH TLV Other Umlls
Recommended
Non-hazardous •------------•------------
•------------
1------------
1 --------------------------·----------------Section Ill -Physlcal/Chemlcal Characteristics
I Boiling Poinl
NIA Specific Gravity (H:P • 1)
I
Vapor Prussure (mm Hg.) N/A Melting Point
Vapor Density (AIR • t) N/A Evaporation Role
(Butyl Acetate • 1)
I SOluOllity In Waler
N/A
Appearance and Odor
Granular powder; Buff to brown ~olor; I Section IV -Flre and Explosion Hazard Data
Flash Point (Method Used)
Musty odor
Flammable Umlt.!,
A I Ex1inguishing Media Foam, CO2,
N A
Dry chemical, Water
Special Fire Fighung Proceduros
I N/A
0.5-0.7
N/A
N/A
LEL UEL
---c-=:---c-=,--,.--------------------------------U nu su al Fire and Explosion Hazards , ______ ..:.:_N:.._;/A ________________ _
I FEB-15-90 THU e.~~ POLYBAC CORP PA P • 1 2
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0 Soction V -Reactivity Data
SIallmty Unstaole Condilions 10 Avoid A . d to moisture. Do not wet. vo, exposure
Stable
y c::+---.... 1i1•r _ 111~•r
Incompatibility {Msterlsl• to Avolcf) Water
Hazardous Decomposillon or ByprOducis N/ A
Hazardous
Polymenza1Ion
May Occur C<lndlUON; 10 Avoid
Will Nol Occur
X
Section VI -Health Hazard Data
Aoute{s) of Entry: Inhalation? Skin? Ingestion? Dust Avoid direct skin contact. Do not ingest.
HfWfo{t8A¥1b~~ur~f8i'a'~"/,¥ove subject to fresh air, SKIN-wash thoroughly with soap and
water, remove and wash clothes. INGESTION-avoid, call physician, observe for 48·
hours for development of allergic symptoms.
Carclnogonlclty: N/A NTP? N/A IAAC Monographs? N/A OSHA Aegula!od? N/A
Signs and Symp1oms of Exposure
Irritation ta the eyes and respiratory tract,
MeaIca1 Condlllons
GenerrutyAggrava1oobyExposure Respiratory allergy (hay·fever, asthma) in some people.
Indiyiduals hyperallergic to enzymes or related proteins should not handle.
Emeraency and First Aid Procedures Wash nands with soap and water. Contact physician immediately, if allergic symptoms
occur in 0-12 hours,
Section VII -Precautions for Sale Handling and Use
Slops to Se Taken In Ca,ie Material Ip R•l~asod or SpiUO<j! Sweep up/collect: avo1d airect skin contact, inhalation and/or ingestion of dust.
'1Foi~~ ~f~Wsal rrethod. Staroard larofi 11 disposal according to legal State and Federal regulations.
May be se..-ered; carpatible with all biological treatrrent net.hods,
Precautions to Be Taken in HB/1dling and Storing Wear respiratory/surgical mask or equivalent MESA/NIOSH. Store in dry place at room
temperature (20°). Do not freeze or refridgerate.
01J1or Precautions Wear gloves
Section VIII -Control Measures
Respiratory Protection (Speedy Typo) Yes, surgical mask or equivalent.
ventJlation
1.oca1 i;xhausr V<>< at nnint nf d11st release Special
Mechanical /G6norllf) Oll!o,
Prot&d!ve Gloveo Yes· I Eye Protect1or, Yes (dust) ANSI-287, 1, 1968.
Olher Protective Clothing or Equipment
Dusting pratectiao -Eyewash f Won</Hygienlc Practices
Co not ingest. Avoid dust. Wash hards with soap ard water after use. Individuals h)?9rallergic to enz)IT'eS
or other related proteins should not handle. Page 2 • u•o•.o",..••n-m"'"'
I
THE MICROTOX®
I INSTRUMENT
I ACCESSORIES AND SUPPLIES
I
WHAT MICROTOX DOES
I The Microtox system measures the toxicity of aque-
us solutions. In the test, a light emitting reagent is
exposed to samples of material in small cuvettes. If the
~
mples are toxic, the light output of the reagent is
duced in proportion to the toxicity of the samples.
he light loss is a simple measure of toxicity. The
lignificance and applications of Microtox, The Fast
oxicity Test•m, are treated at length in other literature.
THE MICROTOX INSTRUMENT
I The Microtox Model 2055 Toxicity Analyzer
mploys a sensitive photomultiplier to measure the
light emitted by small volumes of glowing reagent.
l Separate refrigeration units maintain samples and
agent at precise temperatures.
A patented turret mechanism allows the cuvettes
■ontaining test materials to be exposed conveniently to llie photomultiplier in a brightly lighted lab without am-
bient light leakage.
IJsE OF THE INSTRUMENT
The Microtox Instrument serves both as an experi-
a,ental laboratory instrument and as a "production"
ltJol. It can be operated easily over a broad range of
sensitivities and temperatures for
(
search. It can be used to run large
umbers of tests reliably, day after day,
onth after month.
IHE CHART RECORDER
Microbics Corporation offers an
simplifies the testing process, (serving as an automatic
timer, for example), greatly reduces the probability of
transcription errors, and aids in final data reduction.
SPECIFICATIONS
Power Requirements: The standard unit operates on
nominal 100-120 volts AC at 50/60 Hz. It is fused for 2
amps. (At startup, when the coolers are working to
bring temperatures from ambient to their set points.
the unit draws about 150 watts. Later, when the coolers
need only maintain the set temperature, the unit draws
about 60 watts.) An export model, running on nominal
220-240 volts AC at 50/60 Hz. is fused for one amp.
Height to the top of the turret: 10 inches.
Width: 18.3 inches.
Depth: 20.7 inches.
Weight: 48 pounds. (about 55 pounds,
· packaged for shipment.)
The unit was designed to sit on a laboratory bench.
Even so, people move Microtox instruments around
freely, using them wherever they are needed. The sys-
tem can easily be carried into the field, and operated in
the back of a station wagon or van, using a small port-
able generator for power.
accessory chart recorder matched to t e analyzer: a ten-inch, single-pen.
anual lift, 1 0mv fixed ( 17 speed)
device in either 120 or 220 volt
/.:-~ ~. ~~ ~•/. ' '
l ersions.
Though the Microtox instrument
is equipped with a three-digit display
■hat shows temperatures and light
!levels. most laboratories rely on the
chart recorder for permanent records
If the tests. Use of the recorder
MtCRCJTCJ•_}• ,:;•~,: • ' •• ' • -• -•
fRT: What we do
I
I
'az•nt Assassmant
ane of the first steps the Environmental
ltipact Section takes in formulating a
hazardous substance strategy is to de-
'
mine the degree of risk to people and
e environment Since the hazard may
eel its target population in several
ways, it is essential that the assessment
Insider all environmental media-air,
rface water, ground water, soil, and
omass.
llhe Section has been conducting hazard-lJis assessments at a variety of spills and
waste sites; each operation produces an
E. easingly refined hazard assessment
nique. This increased sophistication
resulted in improved documentation
of the extent of contamination, a logical
l proach for defining "when clean is
ean enough." and the extent of cleanup
imately recommended.
Ifie Section's approach is firirt to consider
■e hazard itself, then to determine its
probable pathways. Identification of the
E pathway or pathways and the
of the contaminant's migration will
e selection of the most cost-effective
remedial actions. These findings are then
E to develop engineering options for
aining and removing contaminants,
restoring the site.
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Toxicity Testing
In its field activities the Environmental
Impact Section employs a new instru-
ment which rapidly assesses the toxicity
of leachates, treatment system effluents
and ambient water. The portable instru-
ment has been used successfully at a
number of hazardous waste sites and
spills for toxicity screening of waterborne
contaminants, as well as for substantia-
tion of other aquatic bioassay tests. The
instrument operates on the fact that toxic
chemicals in water inhibit the light-
, emitting capacity of certain marine bac-
teria. The degree of light inhibition is
directly proportional to the concentration
of the chemicals.
The instrument's effectiveness is derived
from its portability, its compactness and
its dependence upon regular household
electric current Requiring only four feet of
table top or laboratory bench space, it can
be set up in a command post or a motel
room. Individual samples can be assayed
in 5-15 minutes. This type of speed can be
particularly valuable if the sample is from
stored, treated wastewaters that must be
discharged only after toxicity tests are
completed. The instrument also reveals
the synergistic effects of toxic mixtures,
which cannot be predicted by interpreting
data from chemical analysis.
The data produced by the instrument are
the result of toxic chemicals acting on
various physiological systems of the
marine bacteria. As such, the data do not
have as much ecological relevance as a
bioassay of fresh water fish or macroin-
vertebrates. However, the instrument has
been used enough on certain fish/macro-
invertebrate bioassays to make correla-
tions with specific toxic chemicals.
The Environmental Impact Section is also
developing a portable unit using fresh
water fish and Daphnia (a small crusta-
cean) to generate toxicity information.
This unit will soon be combined with the
rapid assessment instrument to provide a
complete system for defining toxicity in
aqueous hazardous materials.
This new unit consists of modules that
can be transported as baggage on most
airlines and set up in a trailer. Its own
temperature control unit maintains ap-
propriate temperatures for test
organisms.
While the unit's diluter system is espe-
cially designed to produce the concen-
trations of pollutant required for generat-
ing standard TL,. data, it can be easily
modified to perform special tests using
more than one pollutant at a time.
The information generated from these
IINo new instruments can be used to
determine:
• The most environmentally acceptable
means of counteracting effects of
hazardous material discharges.
• The environmental acceptability of the
effluent from various treatment
processes.
• The biological "zone of influence• re-
sulting from the discharges of hazard-
ous materials.
For further information on Environmental
Impact Section services, contact:
Dr. Royal Nadeau
Chief, Environmental Impact Section,
ERT
U.S. Environmental Protection Agency
Edison, New Jersey 08837
201-321-6743
FTS-340-6743
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USE OF A BACTERIAL TOXICITY MEASUREMENT SYSTE~'.
AT HAZARDOUS WASTE SITES
Dr. Royal J. Nadeau, Environmental Response Team, U.S.
Environmental Protection Agency, and Michael Kwiatkowski, Jacobs
Engineering, Inc., Edison, New Jersey.
ABSTRACT
A Bacterial Toxicity Measurement System was used at two hazardous
waste sites for relative toxicity screening purposes. Determining
the relative toxicity of samples was valuable at one site as an
intermediate monitoring assessment technique, while at another
site it was used for initial site assessment purposes. The first
incident involved the migration of phenolic compounds from a
buried entrenchment into a nearby creek. Chemical and Microtox
analyses were performed on surface and groundwater samples to
monitor the effectiveness of a subsurface slurry retaining wall,
emplaced to reduce the seep. The second incident involved the
screening of soil and aqueous samples collected during an Extent
of Contamination Survey. The chemical screening was for volatile
organics by Gas Chromatography. The Microcox screening served as
an adjunct for the qualitative detection oi compounds not detected
by the Gas Chromatography analysis. From the joint data, areas of
probable contamination were determined and used as a general basis
for subsequent analyses and sampling.
Although each incident was unique in terms of contamination,
chemical analysis support, and objectives, the Microcox Toxicity
Analyzer System was both flexible and adaptable in meeting each
sites' needs. The System displayed its usefulness as an on-site
monitoring device. Requiring only minimal technical and manpowe~
support samples were collected, prepped, and analyzed 1n an
eight hour period. The System displayed a broad range sensitiYity
11hich was useful for exam1.n1.ng samples when little or no
information was available. The data provided by the Microtox
Toxicity Analyzer System decreased analysis turn-around time and
reduced the number of samples requ1.r1ng extensive chemica:
analysis, in that a lower analysis priority was assigned co the
non-toxic Microtox samples.
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USE OF A BACTERIAL TOXICITY MEASUREMENT SYSTEM AT -----HAZARDOUS WASTE SITES
INTRODUCTION
Hazardous waste site investigations is a topic which has been the
subject of legislative, public, and scientific concern.
Conducting a hazardous waste site investigation is, at best, a
monlllllentous undertaking. Ic encompasses many stages of planning,
each of which must address multidimensional questions. The
initial site assessment plays a vital role since ic serves as a
blueprint for all project planning. Similarly, . any subsequent
assessments are important for monitoring the effectivenesss of a
plan to determine if any changes are necessary. The most
encompassing assessments have included testing of both chemical
and biological parameters. Yet, such involved testing can often
become entangled in individual constraints of time, cost, and
sens1t1v1ty. The Microtox Toxicity Analyzer System™ developed
by Beckman Instrlllllents, Inc., has been used successfully at two
hazardous waste site investigations in reducing these constraints.
MICROTOX ASSAYS
The versacili:y of the Microcox seems from its fundamental
theories and basic operating principles. The system uses a
special strain of lyophilized bioluminescenc marine bacteria
Photobaccerium ohosohoreum, which are reconstituted co provide a
ready-co-use bacterial suspension. Three reagents, bacterial
reconstitution solution, diluent reagent, and osmotic adjustment
solution, are required in milliliter amounts, and are delivered
with fixed volllllle pipettors. The physical system is comprised of
a housing unit which encases the temperature controlled
incubator/turret assembly, photomultiplier hardware, and a digital
display screen. An optional chart recorder may be used for making
permanent records of the data. Since the bacteria are of a marine
origin all samples muse be adjusted to a salinity range of z: co
9:, which is accomplished by using the osmotic adjustment
solution. After an initial light reading is taken the bacteria
are exposed co up co four serial dilutions of the sampt-e. Tne
system is designed for duplicate simultaneous testing. The
relative light output of the bacteria are measured ac discrete
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time intervals, ranging from five to thirty minutes. These light
readings are used, inturn, to calculate the Normalized Percent
Light Decreases (NPLD's). The NPLD's take into account a
correction for natural die-off, and any decrease in Che light
output of the bacteria is attributed co a deterioration in Che
state of health of the bacterial poyulacion, stemming from Che
presence of toxicants in the sample. ·