DEQ-CFW_00061707

Final Report
C8 Assessment of Toxicity Team (CATT) Report Addendum
Aquatic Life Advisory Concentration for C8
JeWl6e J. rura
Project Manager
l ,t "�
Caitlin Cusack
Project Scientist
October, 2002
Prepared For:
TERA
1757 Chase Avenue
Cincinnati, OH 45223
Prepared By:
Menzie-Cura & Associates, Inc.
1 Courthouse Lane, Suite 2
Chelmsford, MA 01824
L�
M g WAidle
Project Reviewer
DEQ-CFW 00061707

TABLE OF CONTENTS
1.0 INTRODUCTION.............................................................................................................1
2.0 REVIEW OF TOXICITY DATA....................................................................................1
3.0 CALCULATION OF AN ADVISORY ACUTE VALUE.............................................1
3.1 CALCULATION OF AN ADVISORY ACUTE -CHRONIC RATIO .............................................. 3
3.2 ADVISORY CONCENTRATION........................................................................................... 4
4.0 UNCERTAINTY...............................................................................................................4
5.0 REFERENCES..................................................................................................................5
LIST OF TABLES
Table 1 Acute Toxicity of Ammonium Perfluorooctanoate to Aquatic Organisms
Table 2 Chronic Toxicity of Ammonium Perfluorooctanoate to Aquatic Organisms
LIST OF FIGURES
Figure 1 Derivation of an Aquatic Life Advisory Concentration for Ammonium
Perfluorooctanoate
LIST OF APPENDICES
Appendix A USEPA Methodology for Deriving Ambient Water Quality Criteria and Advisory
Concentrations
Appendix B Uncertainty Factors
Appendix C References for Tables
t
DEQ-CFW 00061708

1.0 INTRODUCTION
This report provides an aquatic life advisory concentration for ammonium perfluorooctanoate
(C8) based on available toxicity data from a literature search supplied by TERA. The method
followed USEPA's 1985, Guidelines for Deriving Numerical National Water Quality Criteria
for the Protection ofAquatic Organisms and Their Uses and USEPA's 1986, Guidelines for
Deriving Ambient Aquatic Life Advisory Concentrations.
Currently, there is no water quality criterion, advisory concentration, or general standard for C8
that ensures the protection of aquatic organisms and their uses. The lack of required toxicity data
does not allow the calculation of an ambient water quality criterion. Advisory concentrations
"can be based on fewer data than can water quality criteria for aquatic life because advisory
concentrations are not intended to have as much regulatory impact as ambient water quality
criteria" (USEPA, 1986). Advisory concentrations are conservative estimates of potential effects
levels below which "there is probably no cause for concern about effects on aquatic organisms
and their uses" (USEPA, 1986).
2.0 REVIEW OF TOXICITY DATA
After reviewing the literature and toxicity data provided by USEPA, we summarized acute and
chronic toxicity studies of C8 (Table 1). These data include acute toxicity of C8 to:
• Daphnia magna (a water flea);
• Dunaliella Salina (a freshwater algae);
• Lepomis macrochirus, (bluegill sunfish);
• Oncorhynchus mykiss (rainbow trout);
• Photobacterium phoshoreum (a marine bacteria);
• Pimephales promelas (fathead minnow);
• Selenestrum capricornutum (a freshwater green algae);
• Snails; and,
• microbes.
Chronic toxicity values are available for Daphnia magna and Pimephales promelas (Table 2).
Our review indicated that many of these studies met rejection criteria (shown on page one of
Appendix A) or had invalid results.
Table 1 indicates which studies met the rejection criteria and the reason for rejection. Results of
the rejected studies were not included in the final calculation of the advisory concentration.
3.0 CALCULATION OF AN ADVISORY ACUTE VALUE
The acceptable data (from among those studies that did not meet rejection criteria) do not include
all taxa required to calculate an ambient water quality criteria. (Required taxa are listed on page
one of Appendix A). Therefore, we followed the USEPA 1986 protocol, Guidelines for Deriving
Ambient Aquatic Life Advisory Concentrations, which draws upon the procedures in Guidelines
for Deriving Numerical National Water Quality Criteria, for the Protection of Aquatic Organisms
DEQ-CFW 00061709

and Their Uses (USEPA, 1985). The methodology followed to derive the advisory concentration
is shaded pink in Figure 1.
The estimate of an advisory concentration requires data from acceptable acute tests with at least
three animal species. The recommended taxa are:
one fish species in the class Osteichthyes;
one invertebrate species in the class Crustacea; and
one invertebrate species in the phylum Mollusca or a different family of the
Arthropoda.
The available literature does not offer an invertebrate species in the phylum Mollusca or a
species from a different family among the Arthropoda. Therefore, we used test results that:
• did not meet the rejection criteria (as explained in Table 1); and,
• were from the required taxa, although we did not have species from all the
recommended taxa (these studies are highlighted in Table 1).
These results were from tests on three species in the class Osteichthyes and one in the class
Crustacea:
• Lepomis macrochirus (bluegill sunfish);
• Oncorhynchus mykiss (rainbow trout);
• Pimephales promelas (fathead minnow); and,
• Daphnia magna (water flea).
Appropriate effect level values were selected from acute tests according to the criteria listed on
page 2 of Appendix A. Species Mean Acute Values (SMAVs) were calculated as:
Where:
SMAV = {n; * n(i-l)... n(;+,)J'/"
n = acceptable acute value from a specific study;
x = number of studies available.
The specific SMAVs for the selected species are:
Daphnia magna (based on 48 hr EC50 and LC50 values)
SMAV = (360*266*632*221)"= 340.0740 mg/L
Lepomis macrochirus (based on 96 hr LC50 values)
SMAV = (569*634*420)"3 = 533.1101 mg/L
2
DEQ-CFW 00061710

Oncorhynchus mykiss (based on 96 hr LC50 value)
SMAV = 800 mg/L
Pimephales promelas (based on 96 hr LC50 values)
SMAV = (301 *741 *440*766*843)" = 575.9420 mg/L
Because there is only one SMAV available for each genus, SMAV = GMAV. An Advisory
Acute Value (AAV) was calculated by dividing the lowest GMAV (340.0740) by the appropriate
uncertainty factor of 10, which is directly proportional to the number of available GMAVs. The
table of uncertainty factors is in Appendix B.
AAV = 34.0074
3.1 Calculation of an Advisory Acute -Chronic Ratio
There are acceptable chronic values available for Daphnia magna and Pimephales promelas,
(Table 2). Appropriate effect level values were selected from chronic tests according to the
criteria listed on page 3 of Appendix A. The acute -chronic ratios (ACRs) are:
Daphnia magna — 48 hr EC50 = 266 ;z� 7
21-day EC50 (young reproductive impairment) 38
Daphnia magna — 48 hr EC50 = 266 ;:z� 7
21-day EC50 (adult mortality) 40
(species mean ACR for Daphnia magna � 7)
Pimephales promelas - 96 hr LC50 = 766 � 8
48 hr post -fertilization to 30 days post -hatch 100
(hatchability of egg; survival and growth of fry)
The method requires at least 3 ACRs from at least 3 species of aquatic animals from three
different families to calculate an Advisory Acute -Chronic Ratio (AACR). The literature offers
only 3 ACRs from two different species. Therefore we assumed an AACR of 25 to calculate the
advisory concentration, based on Kenaga (1982). This study of the relationship between the
ACR and the type of chemical, type of species, etc, found that 93% of the 30 industrial chemicals
investigated had an ACR </= 25. Therefore, Kenaga (1982) determined that "the use of ACRs of
25 or less appears to be a good tool for predicting the chronic toxicity from the acute toxicity for
organic industrial chemicals." In addition, Kenaga (1982) found that there was no large variance
in ACR values between species. He calculated an average ACR for daphnia (11), sheepshead
minnow (15), coho salmon (5), and fathead minnow (13) exposed to 30 industrial organic
chemicals. In most cases, the ACR for daphnia and fathead minnows was very similar for a
given chemical. The similarity of our ACR values for daphnia and fathead minnows, 7 and 8
respectively, are consistent with Kenaga's finding. Therefore, the use of an AACR of 25 appears
3
DEQ-CFW 00061711

to be consistent with other studies performed on organic chemicals and is a conservative estimate
of the chronic toxicity of C8 to aquatic organisms.
3.2 Advisory Concentration
The advisory concentration is calculated as the Advisory Acute Value/ Advisory Acute -Chronic
Ratio.
Advisory Concentration = 34.0074/25 = 1.3603 mg/L,
USEPA (1986) requires that the advisory concentration be reported using the following
language:
If the measured or estimated ambient concentration of ammonium perfluorooctanoate
exceeds 1.36 mg1L in fresh or salt water, one or more of the following options must be
completed as quickly as possible:
1) obtain additional data concerning the concentration of ammonium
perfluorooctanoate in the effluent and/or ambient water;
2) obtain additional laboratory and/or field data on the effect of ammonium
perfluorooctanoate on aquatic organisms and their uses so that a new aquatic life
advisory or a water quality criterion can be derived;
3) conduct acute and/or chronic toxicity tests on the effluent;
4) reduce the concentration.
After a reasonable period of time, unless a consideration of all available data concerning the
ambient concentration and the effects of ammonium perfluorooctanoate on aquatic life
demonstrate that the ambient concentration is low enough, it must be reduced.
4.0 UNCERTAINTY
Due to the lack of a sufficient amount of data, we were unable to calculate ambient water quality
criteria for C8. It is important to recognize that the calculated advisory concentration of 1.36
mg/L of C8 should not be used as a regulatory standard and is based on a measurable degree of
uncertainty. The data set was limited in the amount of acceptable acute and chronic results and
the number of species represented. In fact, we had to modify the procedure for calculating the
advisory concentration to include acute tests from only one invertebrate species, instead of two.
Further studies on the toxic effects of C8 to different species from different families required by
USEPA (1985) could eventually lead to the development of ambient water quality criteria for
C8.
M
DEQ-CFW 00061712

5.0 REFERENCES
Kenaga, E.E. 1982. Predictability of chronic toxicity from acute tests of chemicals in fish and
aquatic invertebrates. Environmental Toxicology and Chemistry. Vol. 1:pp.347-358.
USEPA, 1985, Guidelines for Deriving Numerical National Water Quality Criteria for the
Protection of Aquatic Organisms and Their Uses. United States Environmental
Protection Agency, Office of Water Regulations and Standards Criteria and Standards
Division, Washington, D.C. and Office of Research and Development, Environmental
Research Laboratory, Duluth, MN.
USEPA, 1986. Guidelines for Deriving Ambient Aquatic Life Advisory Concentrations. United
States Environmental Protection Agency, Office of Regulations and Standards Criteria
Division, Washington, D.C. and Office of Research and Development, Environmental
Research Laboratory, Duluth, MN. NTIS 82212-86-100.
DEQ-CFW 00061713

TABLES
DEQ-CFW 00061714

Table 1
Acute Toxicity of Ammonium Perfluorooctanoate to Aquatic Organisms
West Virginia Ecological Toxicity Study
Test Species
Ex osure Route
Effect Endpoint
Exposure Duration
Effect Level
Compound
Effect Concentration
Nominal Test Concentrations
Reasons for Rejection of Results
Reference
Daphnia magna
In water
48 hr (static, acute)
EC50
FC-143
126 (86-183) mg/L to
01 10, 30, 100, 300, 1000 mg/L
(5182); 0, 10, 100, 500, 1000
Results rejected because of inconsistency: 48
hr EC50 from May, 1982 = >1000 mg/L; 48 hr
22
>1000 mg/L
(6/82)
EC50 from June, 1982 = 126 mg/L.
Daphnia magna
In water
48 hr (static, acute)
EC50
FC-143
360 (219-621) mg/L(6)
0, 430, 730, 1200, 2000, 3330
m /Lts)
35
Daphnia magna
In water
48 hr (static, acute)
EC50
FC-143
266 (231-307) mg/L
0, 25, 40, 63, 100, 160, 250,
23
400,630 m /L
Results not included because the 48 hr EC50
value from the same study is used in the
Daphnia magna
In water
24 hr (static, acute)
EC50
FC-143
416 (366-472) mg/L
0, 25, 40, 63, 100, 160, 250,
advisory concentration calculation. According
23
400, 630 mg/L
to USEPA (1985), 48 hrs is a more appropriate
acute exposure duration for daphnia than 24
hrs.
Daphnia magna
In water
48 hr static, acute
LC50
FC-143
632 570-699 m /L
7.5, 10, 18, 32, 56 m /L
4
Results rejected because there is no reference
Daphnia magna
In water
48 hr (static, acute)
LC50
FC-143
720 mg/L
made to the study from which the results were
36
obtained. Therefore, it is impossible to confirm
the validity of the test methods & results.
Daphnia magna
In water
48 hr (static, acute)
EC50
FC-126
221 (186-261) mg/L
0, 100, 180, 320, 560, 1000
27
m /L
50% growth
Results rejected because there is no reference
(DuPont Co.
Diatoms
In water
reduction; safe
FC-143
2400 mg/L; 720 mg/L
made to the study from which the results were
Unpublished
level (7 days)
obtained. Therefore, it is impossible to confirm
data) in Ref
the validity of the test methods & results.
3
Results rejected because there is no reference
(DuPont Co.
Dunalie/la salina
in vitro
C-8
The algae did not die, but
made to the study from which the results were
Unpublished
also did not grow.
obtained. Therefore, it is impossible to confirm
data) in Ref
the validity of the test methods & results.
3
Results rejected because there is no reference
(DuPont Co.
Lepomis macrochirus
In water
48 hr (static, acute)
LC50
FC-143
1550 mg/L
made to the study from which the results were
Unpublished
obtained. Therefore, it is impossible to confirm
data) in Ref
the validity of the test methods & results.
3
Lepomis macrochirus
In water
96 hr (static, acute)
LC50
FC-143
569 (500-636) mg/L
0, 420, 560, 750, 1000, 1350
4
4,16
m /L
Lepomis macrochirus
In water
96 hr (static, acute)
LC50
H-19704
634 (567-725) mg/L
0, 262, 328, 410, 512, 640, 800,
7
1000 m /L
Lepomis macrochirus
In water
96 hr (static, acute)
LC50
FC-143
>420 mg/L
0, 135, 180, 240, 320, 420 mg/L
15
Oncorhynchus mykiss
In water
96 hr (static, acute)
LC50
FC-118
800 (665-986) mg/L(s)
0, 625, 1250, 2500, 5000, 10000
6
m /L
Photobacterium phosporeum is a single -celled
Photobacterium
organism. According to USEPA (1985) studies
phoshoreum
In water
% light loss
30 min (static, acute)
EC50
FC-1015-X
1950 (1760-2160) mg/L
0, 416, 832, 1665, 3330 mg/L
using single -celled organisms should not be
31
used to calculate an ambient water quality
criteria or advisory concentration.
Photobacterium phosporeum is a single -celled
Photobacterium
organism. According to USEPA (1985) studies
phoshoreum
In water
% light loss
30 min (static, acute)
EC50
FC-118
3150 (2910-3420) mg/L
0, 625, 1250, 2500, 5000 mg/L
using single -celled organisms should not be
30
used to calculate an ambient water quality
criteria or advisory concentration.
Page 1 of 3

Table 1
Acute Toxicity of Ammonium Perfluorooctanoate to Aquatic Organisms
West Virginia Ecological Toxicity Study
Test Species
Exposure Route
Effect Endpoint
Exposure Duration
Effect Level
Compound
Effect Concentration
Nominal Test Concentrations
Reasons for Rejection of Results
Referencelbl
Photobacterium phosporeum is a single -celled
Photobacterium
phoshoreum
In water
% light loss
30 min (static, acute)
EC50
FC-143
730 (630-850) mg/L
0, 125, 250, 500, 1000 mg/L
organism. According to USEPA (1985) studies
using single -celled organisms should not be
29
used to calculate an ambient water quality
criteria or advisory concentration.
Photobacterium phosporeum is a single -celled
Photobacterium
phoshoreum
In water
% light loss
30 min (static, acute)
EC50
FC-126
870 (810-930) mg/L
0, 420, 560, 750, 1000 mg/L
organism. According to USEPA (1985) studies
using single -celled organisms should not be
26
used to calculate an ambient water quality
criteria or advisory concentration.
Pimephales promelas
In water
96 hr (static, acute)
LC50
FC-126
301 (244-370) mg/L
0, 100, 180, 320, 560, 1000
mg/L
28
Pimephales promelas
In water
96 hr (static, acute)
LC50; NOEC
FC-1015
741 (630-999) mg/L;
0, 530, 830, 1330, 2100, 3330
mg/L
33
Pimephales promelas
In water
96 hr (static, acute)
LC50
FC-26
440 mg/L
0, 50,125, 250, 375, 500 mg/L
14
The source refereces a 3M report dated
Pimephales promelas
In water
96 hr (static, acute)
LC50
FC-143
740 mg/L
3/27/96. This report is unavailable therefore,
the results are rejected because it is not
36
possible to confirm the validity of the test
method and results.
Pimephales promelas
In water
96 hr (static, acute)
LC50
FC-143
766 (743-789) mg/L
0, 560, 650, 750, 870, 1000
mg/L
19
Pimephales promelas
In water
96 hr (static, acute)
LC50
FX-1001
843 (811-878) mg/L
0, 690, 750, 810, 870, 930 mg/L
24
Pimephales promelas
In water
96 hr (static, acute)
LC50
FC-143
70 mg/L
0, 10, 20, 30, 40, 50 mg/L
These results are rejected because the LC50
was extrapolated from an insufficient number
13
of data points.
Plant data are not used in the calculation of an
Selenestrum
capricornutum
In water
Algal growth
response
96 hr (static, acute)
EC50
FC-1015
1980 (1710-2360) mg/L
(cell count); >3330 mg/L
021030
, ,430,830,1670, 33
advisor Y concentration because, according to
USEPA (1986), most aquatic plant species are
34
(growth rate)
m30
protected if aquatic animal species are
protected.
Plant data are not used in the calculation of an
Selenestrum
In water
Algal growth
96 hr (static, acute)
NOEC
FC-1015
210 mg/L (cell count); 430
0, 210, 430, 830, 1670, 3330
advisory concentration because, according to
capncornutum
response
mg/L (growth rate)
mg/L
USEPA (1986), most aquatic plant species are
34
protected if aquatic animal species are
protected.
Plant data are not used in the calculation of an
Selenestrum
In water
Algal growth
96 hr (static, acute)
LOEC
FC-1015
430 mg/L (cell count); 830
0, 210, 430, 830, 1670, 3330
advisory concentration because, according to
capncornutum
response
mg/L (growth rate)
mg/L
USEPA (1986), most aquatic plant species are
34
protected if aquatic animal species are
protected.
Plant data are not used in the calculation of an
Selenestrum
capricornutum
In water
Algal growth
9 g
response
96 hr (static, acute)
EC50
FC-143
49 (28-75) mg/L (cell
count); 149 (57-340) mg/L
0, 100, 180, 320, 560, 1000,
advisory concentration because, according to
USEPA 1986 most aquatic plant species are
( ) q p
21
cell dry weight
1800 mg/L
,
protected if aquatic animal species are
protected.
Plant data are not used in the calculation of an
Selenestrum
capricornufum
In water
Algal growth
9 9
response
168 hr (static, acute)
EC50
FC-143
30 (21-40) mg/L (cell
count); 70 (34-118) mg/L
01100, 180, 320, 560, 1000,
advisory concentration because, according to
USEPA (1986). most aquatic plant species are
( ) q p p
21
(cell dry weight)
1800 mg/L
protected if aquatic animal species are
protected.
Plant data are not used in the calculation of an
Selenestrum
capricornutum
In water
Algal growth
9 9
response
240 hr (static, acute)
EC50
FC-143
27 (8-50) mg/L (cell count);
49 (15-96) mg/L (cell dry
0, 100, 180, 320, 560, 1000,
advisory concentration because, according to
USEPA 1986 most aquatic plant s ecies are
( ) q p p
21
weight)
1800 mg/L
,
protected if aquatic animal species are
protected.
Page 2 of 3

Table 1
Acute Toxicity of Ammonium Perfluorooctanoate to Aquatic Organisms
West Virginia Ecological Toxicity Study
Test S ecies
Ex osure Route
Effect End oint
Ex osure Duration
Effect Level
Compound
Effect Concentration
Nominal Test Concentrations
Reasons for Rejection of Results
Reference
Plant data are not used in the calculation of an
Selenestrum
caprcomutum
In water
Algal growth
g g
response
336 hr (static, acute)
EC50
FC-143
43 (14-81) mglL (cell
count); 73 (25-147) mg/L
0, 100, 180, 320, 560, 1000,
advisory concentration because, according to
USEPA (1986), most aquatic plant species are
21
(cell dry weight)
1800 mg/L
protected if aquatic animal species are
protected.
Results rejected because there is no reference
(DuPont Co.
Snails
in water
48 hr
LC50
FC-143
820 mg/L
made to the study from which the results were
Unpublished
obtained. Therefore, it is impossible to confirm
data) in Ref
the validity of the test methods & results.
3
These results are not comparable to results
from other tests because the exposure mediurr
Microbes
Activated sludge
Respiration
p
Inhibition
3 hr (static, acute)
EC50
FC-126
>1000 mg/L (38%
inhibition of respiration
2 blanks, 100, 180, 320, 560,
differs. In addition, the other components of
activated sludge are unknown. In addition,
25
rate)
1000 mg/L
microbes are single -celled
gle-celled organisms which
should not be used to calculate an ambient
water quality criteria or advisory concentration.
These results are not comparable to results
from other tests because the exposure mediurr
Microbes
Activated sludge
Respiration
p
Inhibition
3 hr (static, acute)
EC50
FC-1015-X
>3320 mg/L
2 blanks, 420, 840, 1660, 3320
differs. In addition, the other components of
activated sludge are unknown. In addition,
32
mgtL
microbes are single -celled organisms which
should not be used to calculate an ambient
water quality criteria or advisory concentration.
These results are not comparable to results
from other tests because the exposure mediurr
Microbes
Activated sludge
Respiration
Inhibition
7 min (acute)
Inhibitory
effect
FC-143
No acute inhibitory effect.
0, 1000 mg/L
differs. In addition, the other components of
activated sludge are unknown. In addition,
20
microbes are single -celled organisms which
should not be used to calculate an ambient
water quality criteria or advisory concentration.
EC50 - Median Effective Concentration. This is the concentration of the test substance that causes 50% effect on specific characteristics of the test organism (ex. Immobilization of 50% of Daphnia, reduction in algal cell growth by 50% as
compared to controls) after specified exposure period.
IC50 - Mean Inhibitory Concentration. This is the concentration of the test substance that inhibits biological processes of the test organism by 50% (ex. Light production, respiration) after the specified exposure period.
LC50 - Median Lethal Concentration. This is the concentration of the test substance that kills 50% of the test organisms after a specified exposure period.
LOEL - Lowest Observed Effect Level.
NOEC - No Observed Effect Level.
NOEL - No Observed Effect Level.
Daphnia magna -Water flea.
Dunaliella sa/ina - A freshwater algae.
Lepomis macrochirus - Bluegill sunfish.
Oncorhynohus mykiss -Rainbow trout.
Pimephales promelas - Fathead minnow.
Selenestrum capricornutum - A freshwater green algae.
Ammonium perfluorooctanoate is the common name for octanoic acid, pentadecafluoro-, ammonium salt. All compounds listed in this column are synonyms for this chemical.
a> - Values in parentheses represent the 95% confidence interval for the given effect level.
s - Test concentrations refer to the concentrations of the original test substance, not the corrected concentrations for the assumed % purity of ammonium perfluorooctanoate
° - The test substance is FC-1015, a substance of uncertain purity. According to the 3M reviewer, the test substance is most likely composed of a 30% straight carbon chain version of FC-143 in 80% water. Therefore the 48 hr EC50,
1200 mg/L, of FC-1015 was corrected for the assumed purity of 30% ammonium perfluorooctanoate (FC-143).
s - The test substance is H-24216, a 20% solution of FC-118. The 96 hr LC50 for H-24215 of 4001 (3327-4932) mg/L was corrected for the assumed purity of 20% ammonium perfluroroctanoate (FC-118).
The list of references can be found in Appendix C.
Highlighted values indicate that the results were used to calculate an advisory concentration for ammonium perfluorooctanoate.
Page 3 of 3

Table 2
Chronic Toxicity of Ammonium Perfluorooctanoate to Aquatic Organisms
West Virginia Ecological Toxicity Study
Test Species
Exposure Route
Effect Endpoint
Exposure Duration
Effect Level
Compound
Effect Concentration
Nominal Test Concentrations
Observations
Reasons for Rejection of Results
Reference
All surviving 1 st generation
Daphnids appeared normal at
Results not used because there were
Daphnia
14 & 21 days with
14-day EC50 = >60
test termination. Survival in 36 8
not enough chronic studies to develo
magna
g
In water
Adult mortality
renewal every 2 days
EC50
FC-143
mg/L; 21-day EC50 = 4
0, 5, 8, 13, 22, 36, 60 mg/L
60 mg/L treatments was
the appropriate number of ACRs to
23
semi -static chronic
( )
(31-52) mg/L (ai
statistically significant p <!=
calculate an advisory concentration.
co
0.05) from negative control
Instead an assumed AACR of 25 was
rou .
used.
Daphnids in control produced
neonates on day 7.
Results not used because there were
Daphnia
Young
14 & 21 days with
14-day EC50 = 33 (26-
Reproduction is statistically
not enough chronic studies to develo
In water
reproduction
renewal every 2 days
EC50
FC-143
45) mg/L; 21-day EC50
0, 5, 8, 13, 22, 36, 60 mg/L
significant (ie. p </= 0.05) from
the appropriate number of ACRs to
23
magna
impairment
(semi -static chronic)
= 38 (35-42) mg/L(3)
control (D unnett's) at 13, 22, 36
calculate an advisory concentration.
& 60 mg/L test soln. after 14
Instead an assumed AACR of 25 was
days & in 36 & 60 mg/L test soln.
used.
at 21 days.
No adverse
Hatchability of
effect (no
3M reviewer notes a lack of
Results not used because there were
In water
egg; Survival &
growth of fry
48 hr post -fertilization
observed
reductions in
FC-143
100 mg/L
0, 6.2, 12.5, 25, 50, 100 mgJL
information on purity & analysis
of test substance concentrations
not enough chronic studies to develo
the appropriate numbers o dev to
17
promeales
promelas
(early life -cycle
to 30 days post -hatch
hatchability, °!
and preserved fry or frozen fry
calculate an advisory concentration.
test)
survival &
samples.
Instead an assumed AACR of 25 was
sed.
used.
no
Results not used because there wer
Histological -
"significant,
Any changes observed were
not enough chronic studies to develo
Pime hales
p
promelas
In water
tissue change
9
(early life -cycle
48 hr post -fertilization
to 30 days -hatch
demonstrable
FC-143
100 mg/L
0, 6.2, 12.5, 25, 50, 100 mg/L
judged to be minimal and
the appropriate number of ACRs to
18
post
"tissue
consistent with any routine
calculate an advisory concentration.
test )
change
changes seen in healthy fish.
Instead an assumed AACR of 25 was
used.
EC50 - Median Effective Concentration. This is the concentration of the test substance that causes 50% effect on specific characteristics of the test organism (ex. Immobilization of 50% of Daphnia, reduction in algal cell growth by 501/6 as
compared to controls) after specified exposure period.
IC50 - Mean Inhibitory Concentration. This is the concentration of the test substance that inhibits biological processes of the test organism by 50% (ex. Light production, respiration) after the specified exposure period.
LC50 - Median Lethal Concentration. This is the concentration of the test substance that kills 50% of the test organisms after a specified exposure period.
NOEC - No Observed Effect Level.
Daphnia magna - Water flea.
Pimephales promelas - Fathead minnow.
`'I - Ammonium erfluorooctanoate is the common name for octanoic acid, p pentaderafluoro ,ammonium salt. All compounds listed in this column are synonyms for this chemical.
`�1- Values in parentheses represent the 95% confidence interval for the given effect level.
Results derived using the Moving Average Angle Method.
"I - The list of references can be found in Appendix C
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