NC0025496_Effluent Toxicity Test 2016_20160504

CITY OF LINCOLNTON WASTEWATER TREATMENT PLANT
May 4,2016
Teresa Rodriguez
NPDES COMPLEX UNIT
1617 Mail Service Center I ; EUL)""VR
Raleigh,NC 27699-1617 REGENED'
MAY � 9
V�1u=�r 0,�;,lity
Dear Ms. Rodriguez, pptjr0ll, , -cc ttC,'l
Enclosed is a copy of the March 2016 Fathead Minnow Effluent Toxicity Test and the Ceriodaphnia Dubia
Effluent Toxicity Test conducted by the City of Lincolnton Wastewater Treatment Plant, NPDES permit
number NC0025496. The Fathead Minnow Toxicity test was requested to be performed for our pending
NPDES Permit Renewal. The City of Lincolnton passed the Ceriodaphnia Dubia toxicity test, but the
Fathead Minnow test result was invalid.
The lab performing the test indicated that the samples were foamy when shaken and believes this foam to
have caused the food for the minnows to die. Lack of food caused the minnows to have low growth and
mortality. However, we did pass the Ceriodaphnia Dubia test which was taken at the same time as the
Fathead Minnow test.
We repeated the Fathead Minnow test in April and the result was a pass. Since the March test resulted in an
invalid result, we are performing another Fathead Minnow test this week. 1 will forward the May results as
soon as I get them and hopefully this will complete the required alternate species toxicity testing required
for our NPDES permit renewal.
If you have any questions or need any additional information, please do not hesitate to contact me at 704-
736-8960 or by e-mail at: dona1dhul'kt\4t ci.lincolnton.nc.LIS.
Sincerely,
Donald A. Burkey,J .
W WTP Superintendent
City of Lincolnton
[phone]
P.O. BOX 617 • LINCOLNTON, NORTH CAROLINA 28093-0617
PHONE [phone]• FAX [phone]

•rEffluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:4/4/2016
Facility: Lincolnton WWTP NPDES#NC00 25496 Pipe#: 001 County: Lincoln
�Larat n .eritech, IComments Samples foamy when shaken.
X Artemia mortality probable cause of low growth
1g, 03
Signatuie-a�_ at in sponsi a arge weight and mortality.
X
)' �. - ,� / �.f,;:`� •*•TEST INVALID due to multiple
Signature of Laboratory Supervisor statistical inversions.***
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiation Date/Time 3/22/2016 3:44 PM Avg Wt/Surv. Control 0.775 Test Organisms
%Eff. Repl. 1 2 3 4 Cultured In-House
Control Surviving# 10 10 10 10 %Survival 100.0 Outside Supplier
Original# 10 10 10 10
Wt/original(mg) 0.795 0.631 0.882 0.790 Avg Wt(mg) 0.775 Hatch Date: 3/21/16
5.5 Surviving# F 9 8 8 10 %Survival 87.5 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
Wt/original(mg) 0.678 0.428 0.431 0.609 Avg Wt(mg) 0.537
8.25 Surviving# 10 9 10 %Survival 97.5
Original# 10 10 10
Wt/original(mg) Eil 0.437 0.644 0.620 Avg Wt(mg)F 0.582
11 Surviving# 10 10 7 9 %Survival 90.0
Original# 10 10 10 10
Wt/original(mg) 0.560 0.624 0.530 0.505 Avg Wt(mg) 0.555
16.5 Surviving# 10 9 9 10 %Survival 95.0
Original# 10 10 10 10
Wt/original(mg) 0.668 0.537 0.535 0.644 Avg Wt(mg) 0.596
22 Surviving# 8 7 6 30
Survival 60.0
Original# 10 10 10 10
Wt/original(mg) 0.591 0.479 0.261 0.155 Avg Wt(mg)F. 0.372
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.18 / 7.81 7.88 / 7.81 8.26 / 7.83 8.12 / 7.90 18.18 / 7.93 8.07 / 8.04 18.19 / 7.83
DO(mg/L) Init/Fin 7.65 / 6.73 7.39 / 6.62 7.64 16.85 7.71 / 7.48 1 7.80 / 6.93 7.94 / 7.15 7.61 / 6.20
Temp(C)Init/Fin 24.7 / 24.3 24.5 / 24.3 24.1 / 24.5 24.4 / 25.4 1 24.4 / 24.3 24.5 / 24.3 25.0 / 24.3
High Concentration 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.16 / 7.91 8.01 / 7.83 8.06 / 7.93 8.08 18.05 8.12 / 8.07 8.18 / 8.11 8.17 / 7.89
DO(mg/L) Init/Fin 7.81 16.80 7.53 / 6.57 7.71 16.86 7.87 / 7.51 7.97 / 7.04 7.97 / 7.19 7.70 15.80
Temp(C)Init/Fin 25.0 / 24.4 24.8 / 24.3 24.4 / 24.7 24.6 ! 24.3 24.5 / 24.7 24.7 / 25.0 25.3 / 24.9
Sample 1 2 _3 Survival Growth Overall Result
Collection Start Date 3/21/2016 3/23/2016 3/24/2016 Normal YES r'I ChV INVALID
Grab Hom.Var. F1
Composite(Duration) 24.0 24.0 24.0 NOEC 16.5 8-25%,16,5%
Hardness(mg/L) 80 72 80 LOEC 22 .5%,11%,221,
Alkalinity(mg/L) 97 126 130 ChV 19.1 INVALID
Conductivity(umhos/cm) 785 945 959 Method Steel's Dunnett's
Chlorine(mg/L) <0.1 <0.1 <0.1
Temp.at Receipt(°C) 1.1 0.8 0.8 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 993 994 995 996 997 998 5.5 10 12 2.41 2.8527
Hardness(mg/L) 44 46 46 46 42 46 8.25 10 16 2.41 2.3134
Alkalinity(mg/L) 56 55 56 55 55 54 11 10 14 2.41 2.6340
Conductivity(umhos/cm) 222 223 214 208 214 223 16.5 10 14 2.41 2.1396
22 10 10 2.41 4.8305
DWQ Form AT-5 1/04

Meritech, Inc.
Chronic f=athead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: Lincointon #of Organisms per Chamber: 10 Start Date: 3/22/16 Time:
r
NPDES #: NCO025496 Test Vessel Size: 400 ml End Date: 3/29/16 Time: ) " , •5n/r1
Initiated by: /—( Test Solution Volume: 250 ml
Date/Time Fed: 3/22/16 ',(,(_ t Temp. of Stock: °C Randomization: Yes No
Date/Time Born.- 3/21/16 3:00 PM CT #of Reps: 4 Incubator#:
Organism Source: Aquatox, Inc. Analyst(s): MR, TH, CD
Transferred by: Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials
Day 1 3/23 Day 0 3/22
Day 2 3/24-- ��, C J-,,� �� j Day 1 3/23 '� .���;�f► /`�L- C.' ; 1
Day 3 3/25 Day 2 3/24 ;, C
Day 4 3/26 I `1?..��. /'Y'� Day 3 3/25
Day 5 3/27 Day 4 3/26
Day 6 3/28 �, . '�� Day 5 3/27
Day 3/28 L
Test Termination Data: r 1
Initials/Signature:
End Date: 3/29/16
End Time: 114
/

r' AEM®7
ENVIRONMENTAL LABORATORIES
A Division of Technology and Controls, /nc.
Chemical and Physical Determinations
I
Client: Lincolnton NPDES#: NCO025496 Start Date: 3/22/16
Test Organism: Pimephales promelas End Date: 3/29/16
��'t 3 .3�t-EC: -�1� •� Day
Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Freshwater '}4 _ ,
BatchCS
1. /
pH: Initial `r E:Z ;~, �' r:Z /3
ti
Final �; , ; 7 Y> `( � i a
D.O.. Initial7-
.
,may ,�i 7
Final `'r' , Is-
Temp.: Initial
Final �� n 3 �'�l `T i 2 tet. 13
Conductivity: Initial
Final % r -} - -
Residual Chlorine: Gam, t `r'.- 4. t - % L' Z,
Hardness: L4 j,, L f
Alkalinity:
Day
Concentration: 5.5% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial y G
Final -�
D.O.: Initial
Final r„, �i �, 5�-; �: � 7 i ri•S 1 , tom 1 —
Temp.. Initial
Final
Conductivity: Initial
Final
Residual Chlorine: t _' r t — . L”
Day
Concentration: 8.25% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial
Final `1 i 7) fl
-` - r
D.O.. Initial } :��-1 � '�`t r, .1`1
Final z:
Temp.. Initial
Final }
Conductivity: Initial ..
Final _ -
.r
Residual Chlorine: L .+ I ` C 1 z-

• „ /!//IIER/T�CH� /N�
ENVIRONMENTAL LA13ORATOR1F-! c
.0 Oivisicra c/�a nrcr 1x+chrao/opy wr.rf Co.a�rcais, i
Chemical and Physical Determinations
Page 2 of 2
Client: Lincolnton NPDES#: NCO025496 Start Date: 3/22/16
Test Organism: Pimephales promelas End Date: 3/29/16
Da
Concentration: 11% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial "6 1 , t� �s G ,�• C-� C': r �� l'vt cl`i, 4
Final 1.� �� '7r vi-- 7- 7 l i CX �?
D.O.. Initial `1.4�' 1 (..G — r'%` 7.rl
Final
Temp.: Initial
Final ;_fit t. 1 ��:4 `' 'J j Lj 7 % '+
Conductivity: Initial
Final i .<
Residual Chlorine: �-c A
Day
Concentration: 16.5% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial
Final ,' r. -S";
D.O.: Initial ) 1i� �'1 11 '.-1 7, ? `i 7l 7 1.Lr
Final r, �✓ �-;(�,`; �- ' 7
Temp.- Initial Z1C1�
Final
Conductivity: Initial j'� -l; �� '�j j� �' s
Final �";% "� =' �``t s' ' % _� I[- c -7 I
Residual Chlorine: _ ( Vic.- <
Day
Concentration: 22% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial
Final "� t "1 7 -1
-
D.O.: Initial
Final
Temp.- Initial
Final w, Z ;_ •_ !
Conductivity: Initial
Final .=
Residual Chlorine: `v
Day
Concentration: 100% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
PH
Conductivity: -
Residual Chlorine:
Hardness:
Alkalinity: t 1 t ��( _ ��C'

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lincolnton NPDES#: NC0025496 Start Date: 3/22/16
Test Organism: Pimephales promelas End Date: 3/29/16
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
V
Rep A 0
Rep B 0
Rep C 0
Rep D 0 L
Concentration 5.5%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
i'
Rep A 0
Rep B 0
Rep C 0
Rep D 0
Concentration 8.25%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 - r
Rep B 0
Rep C 0
i
Rep D 0 — L;

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lincolnton NPDES#: NC0025496 Start Date: 3/22/16
Test Organism: Pirnephales promelas End Date: 3/29/16
Concentration
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
G.
Rep A 0
Rep B 0
i
Rep C 0
Rep D 0
Concentration 16.5%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
0
Rep A / L., L'
Rep B 0
Rep C 0
Rep D 0
Concentration 22%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 i
Rep B 0
Rep C 0 `-
Rep D 0 r

ENVIRONMENTAL LABORATORIES
-•"^," A Division of:Vater Technology and Controls, lnc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lincointon Page: 1 of 2
NPDES #: NCO025496 Outfall:
Date of Test: 3/22/16
Initial weights taken on: J by: Organism: Pimephales promelas
Final weights taken on: by:
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONIC REP (mg) WT. (mg) (mg) #ORG. WTJORG. (mg) SURV%
+ B
C
O
C
A
5.5% B
D
8.25%
C - ✓
D

ENVIRONMENTAL LABORATORIES
'1- A Division of Vl'a Cer T—h-1.9y.end Conirn/s, /nc
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lincolnton Page: 2 of 2
NPDES #: NCO025496 Outfall:
Date of Test.- 3/22/16
Initial weights taken on: by: C Organism: Pimephales promelas
Final weights taken on: ' by: 0
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) # ORG. WTJORG. (mg) SURV%
11%
D
�.
4 `.
16.5% B _ ,
C - -7
22% B ✓

9P ERI11 ECH, INC. MeritechSample ID
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: c, L�� U2�c cd>a-loa Wo iTP PO#: f9, 396-, ,3
Contact Person: � {� 'tT U-mN .TI/=._j NPDES#: NC 0o—a �'4Cr (ya
Address: � + p ,C ( ('� Phone: 9/74, '7.3C�, �>(o b
City: U t o Imo' +'l Pipe#: _ (�
County: 1-�r�t�(} State: C- Zip: IS-1✓93
SAMPLE INFORMATION
I - -
Sample Site: t
Sample Type: ❑Grab Composite #of containers:
Sampling Time: Start Date: Start Time: s� AM PM 1 y
End Date: End Time: AM PM
.i. :�` :til', ..i5 ' ..:_% r ,-•.: r - !:^- r-( -. .. Ali^G `-�`n"� C :. � _ ... _. _ _ (;";�..,•
Collector's Name. Print Signature:
TOXICITY TEST INFORMATION
Test Required: ❑ Chronic(7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) Pimephales promelas (fathead minnow)
JJJ❑��� Mysidopsis bahia (shrimp)
MC: % Test Concentrations(if multiple dilutions):
Comments:
A + _
11 SHiiPPING INFO6=MIATION
Relinquished by: ,L_ Date: - �1—� Q-, Time: PM
Received by`;, / n - .�- J Date: t�, ryr- — ! Time: l�.� 0MA PM
Relinquished byl--" ✓ Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature('C):
Method of Shipment: ❑UPS []Fed EX EXMeritech Pick-up ❑ Delivered Other
C��I .c`',i.; ..1.;_.'�' _ �c:U: m'i'l.. .,� `: \ _. .. �...
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by; <
Received by: j Date: - Time: I AM PM
Sample Temperatures(°C): / / / Sample Condition: 'r
= L i_ L _

MERI 1 ECH, INC. Meritech Sample 10#:
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reedCa)meritech-labs.com Web Site: www.meritech-labs.com
/y CLIENT INFORMATIONClient: I t 1 r\Lojn h)n W ,w t t PO#:
Contact Person: �ty1Y\� � n �j,;�.� rte: <V , NPDES#: NC rj(-)2L544
Address: 'PQ �jvx (�('� Phone: 19 0�4 /) 3 L, (" C)
City: Pipe#: 00
County: J ;n r; i; State: A/ Zip: R:L�3
SAMPLE_ INFORMATION
Sample Site:
Sample Type: ❑Grab omposite #of containers: - c
_ t G
Sampling Time: Start Date: 3 Start Time: � 75 AM PM
End Date: L End Time: y � ASI PM
***Triple rinse sample container with sample before filling. Completely Fill the sample container with no air space.
Pack the sample cooler completely in ice- The sample must be<6ArC capon receipt at the Iabo tory—
Collector's Name: Print: ��1t1 t`t ���`� Signature: <oe,�
r V_- �4
TOXICITY TEST INFORMATION
Test Required: ❑Chronic(7 days) Test Organism: XCeriodaphnia dubia (water flea)
❑Acute(24-48 hours) 1>�imephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by— Date: 3 �—y,p Time: �1--'� AM PM
Received by: �� Date: 3—4;1 Time: Z AM PM
Relinquished by: E Date: J�� �� �� Time: 3"!a CP APA
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: APA PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX r1 Meritech Pick-up Delivered Other
**Samples shipped on Friday must be FedEx and must beclearlylabeled for Saturday delivery,NO SIGNATURE REQUIRED"
SAMPLE RECEIVING(Laboratory Use Only)
c
Relinquished by:
Received Date: 3 ���G Time: J AM (Full 1I
Sample Temperatures( ): I(.!,`� I I Sample Condition:
WHITE=Laboratory copy YELLOW=Client copy

MERI Y E""f INC. Meritech Sample ID#: r�.� �) ! f. � t;
_ Bioassay Sample Chain of Custody
1r�p 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reed(a)meritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: 4-t►1 Ltu�► ) rA,! - PO#:
Contact Person: Vq V ` =,a vV•G 4=e-1 NPDES#: NC 0C>;l. S LC, (e,
Address: F0 Goy,X�("0 1 9 Phone: "7()-; /23 6,
City: 1 'j r\ co I nv ty r) Pipe#: 00 �
County: 4=�n{' o l n State: ,�! � Zip:
SAMPLE INFORMATION
Sample Site: /: 1(—C,
Sample Type: ❑Grab Composite -- #of containers:
Sampling Time: Start Date: �j '� C, StartTime: �lr` AM; PM
End Date: '�-� -�Sc' End Time: 1(�1 �4 AM ! PM
T ri.ple.-.*,-,se sa;7%ife cc.r7,aif7er t,'i_h sarp-!e hafr_-e r71Nr;g. co;nprefely 1 Z!ta SEjmP!e co.atc'i:,S; I.- nic E1r spece.
Poch the sEmDfe cooler cQRt;Jr2L1e1y ir!ice. The sample rr ci.:7_ <6-OoC ufj n ecei,')t Pt .se ecru^'rc':C•_�r"/�
Collector's Name: Print: •�
111 {' r 'a\ Signature:
f
TOXICITY TEST INFORMATION
Test Required: ❑Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) *imephales promelas (fathead minnow)
❑ Mysidopsis Bahia (shrimp)
IWC: I % Test Concentrations(if multiple dilutions):
Comments'
SHIPPING INFORMATION
Relinquished¢ f._SC Date: ,j 7 ; Time: f� .j,� Aivl// PM
( i
Received hy_: j�j Date: Time: /��r PM
Relinquished by: %y� /r�si'r� Date: E 'r j_"��/ Time: l5 f AM PM
Received by: i Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX FN/Meritech Pick-up ❑Delivered ❑ Other
Samples shipped on Friday must be_ and mus'be clearly labeled for Sa°urda•de!"svS ,' '- PE REZJIIIRED
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
Received by: Date: Time: S: AM M
r`
Sample Temperatures(°C): I ! I Sample Condition:

Title: Lincolnton
File: Lincolnton Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
----------------------------------------------------------------------------
D = 0.2506
W = 0 . 9799
Critical W = 0 . 8840 (alpha = 0 . 01 , N = 24)
W = 0 . 9160 (alpha = 0 . 05 , N = 24)
----------------------------------------------------------------------------
Data PASS normality test (alpha = 0 . 01) . Continue analysis .

Title: Lincolnton
File : Lincolnton Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 5 . 7239 (p-value = 0 .3340)
Data PASS B1 homogeneity test at 0 . 01 level . Continue analysis .
----------------------------------------------------------------------------
Critical B = 15 . 0863 (alpha = 0 . 01, df = 5)
= 11 . 0705 (alpha = 0 . 05, df = 5)

Title: Lincolnton
File: Lincolnton Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 5 0 .3335 0 . 0667 4 . 7918
Within (Error) 18 0 . 2506 0 . 0139
----------------------------------------------------------------------------
Total 23 0 . 5841
----------------------------------------------------------------------------
(p-value = 0 . 0058)
Critical F = 4 .2479 (alpha = 0 . 01, df = 5, 18)
= 2 . 7729 (alpha = 0. 05, df = 5, 18)
Since F > Critical F REJECT Ho: All equal (alpha = 0 . 05)

Title: Lincolnton
File: Lincolnton Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 1 OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0 . 05
----- -------------------- ----------- ------------------ ------ ---
1 Control 0 . 7745 0 .7745
2 5 . 5% 0 . 5365 0 . 5365 2 . 8527
3 8 .25% 0 . 5815 0 . 5815 2 .3134
4 11% 0 .5548 0 . 5548 2 . 6340
5 16 . 5% 0 .5960 0 . 5960 2 . 1396
6 22% 0 .3715 0 . 3715 4 . 8305
----------------------------------------------------------------------------
Dunnett critical value = 2 .4100 (1 Tailed, alpha = 0 . 05, df = 5, 18)
Title: Lincolnton
File: Lincolnton Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 2 OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
----- -------------------- ------- ---------------- ------ ------------
1 Control 4
2 5 . 5% 4 0 .2011 26 . 0 0 .2380
3 8.25% 4 0 .2011 26 . 0 0 . 1930
4 11% 4 0 .2011 26 . 0 0 .2198
5 16 . 590 4 0 .2011 26 . 0 0 . 1785
6 22% 4 0 .2011 26 . 0 0 .4030
----------------------------------------------------------------------------

Title: Lincolnton Mortality
File: LincMort Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
----------------------------------------------------------------------------
D = 0 .2450
W = 0 . 9144
Critical W = 0 . 8840 (alpha = 0 . 01 , N = 24)
W = 0 . 9160 (alpha = 0 . 05 , N = 24)
----------------------------------------------------------------------------
Data PASS normality test (alpha = 0 . 01) . Continue analysis .

Title: Lincolnton Mortality
File: LincMort Transform: NO TRANSFORMATION
Hartley' s Test for Homogeneity of Variance
Bartlett ' s Test for Homogeneity of Variance
----------------------------------------------------------------------------
These two tests can not be performed because at least one group has .
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless .
----------------------------------------------------------------------------

Title: Lincolnton Mortality
File: LincMort Transform: NO TRANSFORMATION
Steel ' s Many-One Rank Test - Ho: Control<Treatment
----------------------------------------------------------------------------
MEAN IN RANK CRIT. SIG
GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0 . 05
----- -------------------- ----------- ------- ------ ----- ---
1 Control 1 . 0000
2 5 . 506 0 . 8750 12 . 00 10 . 00 4 . 00
3 8 .2506 0 . 9750 16 . 00 10 . 00 4 . 00
4 110-0 0 . 9000 14 . 00 10 . 00 4 . 00
5 16 . 5% 0 . 9500 14 . 00 10 . 00 4 . 00
6 220-. 0 . 6000 10 . 00 10. 00 4 . 00
---------------------------------------------------------7------------------
Critical values are 1 tailed ( k = 5 )

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50 Date: 03/30/16
Facility: LINCOLNTON WWTP NPDES#: N00025496 Pipe#: 001 County: LINCOLN
La or tr Perf mi Te t: MERITECH LABS, INC.
Comments:
X
Signa r o erato
uResponsible Charge
X
Signature o L orator pervisor * PASSED: 2.61% Reduction
Water Sciences Section - Aquatic
Work order: Toxicology Branch
MAIL ORIGINAL TO:
Division of Water Resources
1621 Mail Service Center 621
North Carolina Ceriodaphnia Raleigh, N.C. 27699-1621
Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t = 0.363
Tabular t = 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction = 2.61
% Mortality Avg.Reprod.
# Young Produced 24 27 24 24 24 20 28 25 30 28 26 27
0.00 25.58
Control Control
Adult (L)ive (D)ead L L L L L L L L L L L L
0.00 24.92
Treatment 2 Treatment 2
Effluent %: 11%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Control CV
10.336% PASS FAIL
# Young Produced 27 32 22 23 23126113 26 27 32 31 17 % control orgs X
producing 3rd
brood Check One
Adult (L)ive (D)ead L L L L L L L L L L L L 1001-.
1st sample 1st sample 2nd sample Complete This For Either Test
pH Test Start Date: 03/23/16
Control 7.87 8.03 7.94 8.04 7.96 8.12 Collection (Start) Date
Sample 1: 03/21/16 Sample 2: 03/23/16
Treatment 2 7.82 8.07 8.01 8.17 8.01 8.09 Sample Type/Duration 2nd
1st P/F
s s S Grab Comp. Duration D
t e t e t e I S S
a n a n a n Sample 1 X 24 hrs L A A
r d r d r d U M M
t t t Sample 2 X 24 hrs T P P
1st sample 1st sample 2nd sample
D.O. Hardness (mg/1) 44 ,,,,,,,, ,,,,,,,,
.......... ..........
Control E76N9
7.82 7.50 7.52 7.54
Spec. Cond. (pmhos) 190 785 945
Treatment 2 7.73 7.59 7.58 7.59
Chlorine(mg/1) ,,,,,,,, <0.1 <0.1
LC50/Acute Toxicity Test Sample temp. at receipt(OC) ,.....,. 1.1 0.8
(Mortality expressed as %, combining replicates)
Note: Please
Concentration Complete This
Section Also
Mortality
start/end start/end
LC50 = % Method of Determination Control
95% Con i en�imits Moving Average Probit
-- % Spearman Karber — Other — High
Conc.
pH D.O.
Organism Tested: Ceriodaphnia dubia Duration(hrs) :
Copied from DWQ form AT-1 (3/87) rev. 11/95 (DUBIA ver. 4.41)

Ma,uoo».mu.
Mini Chronic Pass/Fail Test: Cvriodephmiadu0ia |noubotor#:#Young Produced ( '
Client: Pipe#: County: Date Start: Date End:
NPIDES#: NC Date Time of Culture Transfer: Time End:
Dilution Water: Lake Brandt Date/Time Neonates born: 1 st Renew-al Date:
Test Organism Source: Tray# Age of �eonates at Test Start:,-� 2- hot,r,,; 2nd Renewal Date: Time:
Stirred/Aerated for D.O.: Y /gRandomized:UY N Culture Tray Temp:.,.
Sample 1: Sample 2-. —'
Adults Live/ Dead T-]4�1 I Sample Information
hours
hours
Adults Live/ Dead
Transferred by: Fed by:
Young Produced Batch# Samplel Sample 2 DayAdults Live/ Dead- C- i t-- Transfer Day
Day
Hardness
Day2
I Produced L4
Spec.Cond. Day 3
Percent of Control producing third brood: Day4
'Test Sample Organism Reproduction (mg]L) Day
Receipt Sa�-ple Day � `^
Terminated by:
H 1 st Sample 2nd umple 2nd Sample
#Young Produced Control
Adults Live/ Dead
Sample <6'I
#Young Produced
D C) 1stSample 2nd mple 2nd Sample
3
C, Sample
Adults Live/ Dead L t.- L- initial- final —initial final i-n.t,=a final
Temp. 1 st Sample 2nd Sample 2nd mple
Total Produced Control
�w�
Comments: Sample -,Ik,
ina-iaT- f7nal— final initial final

MERITECH, INC. MeritechSample ID#: ���� •� �
Sioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Chen;: . • . L (\ C ,�f rte'! �A w i r PO#: /93( l(fes .4
S�v1r% l� \i ' ]l - u'—� NPDES#: NC �D D S'' -9
Contact Person: {�
Address: rV P(�i 19
Phone: 904, 7 3�, 6 6
City: t ie\ (—J 1'Y�"�i� Pipe#: _ –
County:
Li�'1L O ti� State. C Zip: cl3
cG SAMPLE INFORMATION
Sample Site: � ` `
Sample Type: ❑Grab Composite #of containers:--� �
Sampling Time: Start Date: 4 ` ! � Start Time:
FA-M)
PM
End Date: �–) to End Time: PM
"•Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample container completely in ice. The sample,must be<6.0°C upon r eipt at the laboratory'
Collector's Name: Print:
Signature:
TOXICITY TEST INFORMATION
Test Required; ❑ Chronic(7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) .Pimephales promelas (fathead minnow)
Mysidopsis bahia (shrimp)
IWC: 1 % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: r_� Date: �c��` Timer PM
Received by:! ✓ Date: _ Time: 6M PM
Relinquished b Date: f� Time: >L– AM PM
Received by: Date:
Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by:
Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment: ]UPS []Fed EX 1XMeritech Pick-up Delivered E] Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery,NO SIGNATURE REQUIRED"
SAMPLE RECEIVING (Laboratory Use OnIV)
Relinquished by: 6 � ^'t"'"`
Received by: Date: a – Time: I U AM P
r / / Sample Condition: `e CA
Sample Temperatures(°C): /
WHITE= Laboratory copy YELLOW=Client copy

IV1ERITECH, INC. MeritechSample10#: `
_ - Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reed(aD_meritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: CI U� t—t�L�;j ��i1 �it1Z � PO#:
Contact Person: J C't1�( L� �Yl� ?�V�,-� NPDES#:
��. J.
Address: �� �irY. (�si'� Phone:
City: Pipe#: )
County: State: A/6- Zip:-9 R-L C1,3
SAMPLE INFORMATION
Sample Site:
Sample Type: ❑Grab omposite #of containers: r - Cl
�=
� � ''iiC c
Sampling Time: Start Date: 3 �3 1`f' Start Time: 7 7.] emw
PM (
End Date: 3 :_2 4 � � End Time: �1� PM
re-'_I�.- � f r^N the c-..� cr_. i C ^rte
c:. -, ::Ir1:F1/E� a)- te`. i..C- c...(�'2@ CC^iaf(tcr'l4"ur RQ c.� flcw.
.c. - r.,-- v The cp, m 's,tc< 000 L'Por::-sce!'Of a'f'»`ie
Z�G.r..�t.�f_e.��.C.:1 J.' vl.'�;'.�-.i V.1.it�:C:E:_ e_�e lve. v.r?'� @.t . n.zz
I w 1
Collector's Name: Print: ��r�y1 ""\-e-- Signature: L'-�Q
TOXICITY TEST INFORMATION
Test Required: ❑Chronic(7 days) Test Organism: Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) '1>epimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished byr-4, LDate: ^-�`�— Time: AM
e
Received by �,��` Date: �� '�.'f�—�` Time: r!��G7 AM PM
Relinquished by: -,jY_e, Date: - ��= %(j Time: ji L AM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX kMeritech Pick-up ❑Delivered ❑ Other
"Sampa es shi;,Ped orrricay must be FedEx,and,mi ust be clearly iabeled for Saturday delivery,€�O SIC€i:4�TURE REOUIF,K)
SAMPLE RECEIVING (Laboratory Use Only)
Relinquished by:
Received 6y Date: `l Time: — AM PM
Sample Temperatures(°C): > /(. / / Sample Condition:
r'J- tt`l i L- Lel}G Ce L�[ GO t' YELLOVir= Client cc-

Effluent Toxicity Statistical Results - Chronic Pass/Fail Date: 03/30/16
Facility: LINCOLNTON WWTP NPDES#: NCO025496 Pipe#: 001 County:LINCOLN
Laboratory Performing Test: MERITECH LABS, INC.
Reduction: 2.611 CONTROL 111 Effluent
# Replicates 12 12
Female Live 12 12
Adult Male 0 0
Adult Dead 0 0
Adult Mortality 0.000-0 0.001
# Neonates 307 299
Mean # Neonates 25.583 24.917
Standard Deviation 2.644 5.791
Coefficient of Variation 10.3361
Fisher's Exact Test
A = 12 B = 12 a = 12 b = 12
a/A = 1.00 b/B = 1.00
Success is: survival
Critical b value = 8
12 > 8
The test concludes that the proportion of survival is not
significantly different for the control and the effluent groups.
Test Passes!
SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA
ORDERED OBSERVATIONS
i Group Neonates Centered i Group Neonates Centered
1 E 13 -11.9167 13 E 26 1.0833
2 E 17 -7.9167 14 E 26 1.0833
3 C 20 -5.5833 15 C 27 1.4167
4 E 22 -2.9167 16 C 27 1.4167
5 E 23 -1.9167 17 E 27 2.0833
6 E 23 -1.9167 18 E 27 2.0833
7 C 24 -1.5833 19 C 28 2.4167
8 C 24 -1.5833 20 C 28 2.4167
9 C 24 -1.5833 21 C 30 4.4167
10 C 24 -1.5833 22 E 31 6.0833
11 C 25 -0.5833 23 E 32 7.0833
12 C 26 0.4167 24 E 32 7.0833

SHAPIRO-WILK'S TEST FOR NORMAL DISTRIBUTION OF DATA (cont.)
COEFFICIENTS AND DIFFERENCES
i x(n-i-1) x(i) a(i) x(n-i-1) - x(i)
1 7.0833 -11.9167 0.4493 19.0000
2 7.0833 -7.9167 0.3098 15.0000
3 6.0833 -5.5833 0.2554 11.6666
4 4.4167 -2.9167 0.2145 7.3334
5 2.4167 -1.9167 0.1807 4.3334
6 2.4167 -1.9167 0.1512 4.3334
7 2.0833 -1.5833 0.1245 3 .6666
8 2.0833 -1.5833 0.0997 3 .6666
9 1.4167 -1.5833 0.0764 3.0000
10 1.4167 -1.5833 0.0539 3.0000
11 1.0833 -0.5833 0.0321 1.6666
12 1.0833 0.4167 0.0107 0.6666
1
W = X 418.1289
445.8333
Calculated W = 0.938 Critical W = 0.884
0.938 z 0.884
The reproduction data is normally distributed
evaluated at a 99% confidence interval.
Test Passes!
F test for Homogeneity of Variance
Effluent variance 33 .5379
F = _ = 4.80
Control variance 6.9924
Numerator degrees of freedom: 11 Denominator degrees of freedom: 11
Critical F = 5.32
4.80 <_ 5.32 =► The Test PASSES, the variances of the two groups
are significantly the same, homogeneous.

EQUAL VARIANCE t TEST
25.6 - 24.9
t = = 0.363
1.838
Degrees of freedom = 22
Critical t = 2.508
0.363 a 2.508
Test passed. There is not a significant difference in reproduction
between the Control and the effluent evaluated at a 99% confidence interval.
Chronic Test PASSES

r
Effluent Toxicity Report Form-Chronic Fathead Minnow Multi-Concentration Test Date:4/22/2016
Fa cil Lincolnton WWTP NPDES#NC00 25496 Pipe#: 001 County: Lincoln
Lab ra et h, InA Comments
X Pill" A
Signature of pe orp nsiote Charge
X
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh,NC 27699-1621
Test Initiation Date/Time 4/12/2016 3:55 PM Avg WUSurv.Control 0.658 Test Organisms
%Eff. Repl. 1 2 3 4 r' Cultured In-House
Fc-o-ni-ro-il Surviving# 10 10 10 10 %Survival 100.0 I✓ Outside Supplier
Original# 10 10 10 10
Wt/original(mg) 0.719 0.698 0.481 0.734 Avg Wt(mg) 0.658 Hatch Date: 4/11/16
5.5 Surviving# 10 10 10 10 %Survival 100.0 Hatch Time: 3:00 pm CT
Original# 10 10 10 10
Wt/original(mg) 0.691 0.649 0.478 0.413 Avg Wt(mg) 0.558
8.25 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.677 0.602 0.661 0.589 Avg Wt(mg)F 0.632
11 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.681 0.576 0.535 0.652 Avg Wt(mg) 0.611
F76-75-1 Surviving# 10 6 9 9 %Survival 85.0
Original# 10 10 10 10
Wt/original(mg) 0.623 0.342 0.579 0.456 Avg Wt(mg) 0.500
22 Surviving# 10 10 10 10 %Survival 100.0
Original# 10 10 10 10
Wt/original(mg) 0.624 0.562 0.699 0.649 Avg Wt(mg) 0.634
Water Quality Data Day
Control 0 1 2 3 4 5 6
pH(SU)Init/Fin 8.23 / 7.90 8.24 / 7.93 8.12 / 7.81 8.23 / 7.86 8.25 / 7.77 8.22 / 8.07 8.23 / 8.04
DO(mg/L) [nit/Fin 7.82 / 6.90 7.86 17.51 8.08 16.93 8.15 7.52 8.20 / 6.55 7.92 / 8.02 8.21 / 7.65
Temp(C)Init/Fin 25.1 125.5 25.1 / 24.3 24.9 / 24.6 25.4 r 24.5 24.3 / 24.6 24.4 / 25.9 25.0 / 24.4
High Concentration 0 t 2 3 4 5 6
pH(SU)Init/Fin 8.10 / 7.88 8.09 / 8.01 8.17 / 8.03 8.14 / 7.93 8.21 / 7.91 8.17 / 8.16 8.19 / 8.14
DO(mg/L) Init/Fin 7.75 / 6.68 7.65 / 7.93 7.96 / 7.02 8.06 / 7.01 8.02 / 6.66 7.92 / 7.85 8.21 / 7.84
Temp(C)Init/Fin 24.1 / 24.7 24.8 / 24.3 25.3 / 25.2 25.1 / 25.2 24.3 124.4 24.6 125.8 25.2 / 24.5
Sample 1 2 3 Survival Growth Overall Result
Collection Start Date 4/11/2016 4/13/2016 4/14/2016 Normal ri Fi ChV >22
Grab Hom.Var. ri 1171
Composite(Duration) 24.0 24.0 24.0 NOEC 22 22
Hardness(mg/L) 32 40 48 LOEC >22 >22
Alkalinity(mg/L) 94 115 148 ChV >22 >22
Conductivity(umhos/cm) 830 866 1078 Method Steel's Dunnett's
Chlorine(mg/L) <0.1 <0.1 <0.1
Temp.at Receipt(°C) 1.0 0.6 0.4 Stats Survival Growth
Conc. Critical Calculated Critical Calculated
Dilution H2O Batch# 1004 10051006 1007 1008 5.5 10 18 2.41 1.4163
Hardness(mg/L) A
44 44 44 44 8.25 10 18 2.41 0.3396
Alkalinity(mg/L) 55 52 54 44 11 10 18 2.41 0.6467
Conductivity(umhos/cm) 211 200 212 212 16.5 10 12 2.41 2.2509
22 10 18 2.41 0.3216
DWQ Form AT-5 1/04

Meritech, Inc.
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: L i n CO i nto n #of Organisms per Chamber: 10 Start Date: 4/12/16 Time:
NPDES#: NCO025496 Test Vessel Size: 400 ml End Date: 4/19/16 Time: 1 i
Initiated by: ��L �(` ` Test Solution Volume: 250 ml
r
Date/Time Fed: 4/12/16 j ( !. �'al,, Temp. of Stock: r �!. -' °C Randomization: Yes No
Date/Time Born: 4/11/16 3:00 PM CT #of Reps: 4 Incubator#:
Organism Source: Aquatox, Inc. Analyst(s): MR, CD C_
Transferred by: Fed by:
Date Time Initials Date Time 1 Initials Time 2 Initials
Day 1 4/13 IL,i' , , Day 0 4/12 ;�. ,ls�,,•.. ��r�._ - ,"�"`! /^ itit�_,
Day 2 4/14 �� „� `�_ :%C..,., Day 1 4/1.3 5
Day 3 4/15 I;?' ?}�,.••� .t')/I 1 G i Day 2 4/14 M
Day 4 4/16 r;t ( r��: v 1� Day 3 4/15
Day 5 4/17 Y' 6 Day 4 4/16 j
Day 4/18 �: 3(%:� _ r),X, Day 4/17
Day 6 4/18
Test Termination Data:
Initials/Signature:
End Date: 4/19/16
End Time:
s

ENVIRONMENTAL LABORATORIES
�' A Division of Wafer Technology and Contrpls, Inc_
Chemical and Physical Determinations
Client: Lincolnton NPDES4. NCO025496 Start Date: 4/12/16
Test Organism: Pimephales promelas End Date: 4/19/16
Day
Concentration: control 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
Soft Synthetic Freshwater / T
Batch#: J L
H: Initial
Final 7V
D.O.. Initial 7-
Final
Final (; 'I! f =,i - ( -5
Temp.: Initial y, 2)
Final ?S .5 -}� _ ��►�, i. 2 ' `�` .r i`t. `t
Conductivity: Initial `~( 2-11 ;; ? ► ^?
Final L4�j T�.,; ,�)�
Residual Chlorine:
Hardness: Lid `' \1 LN U L Lg U
Alkalinity: '
J _}
Day
Concentration: 5.5% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial
Final -7� 71> r r+ 7 ?.
D.O.. Initial
Final L.zz. 7 '�.1 7
Temp. Initial _L .Z Y-c
Final
Conductivity: Initial " Z
Final )_k
Residual Chlorine:
Day
Concentration: 8.25% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial l.2 S-
Final -7 S c"
J !
D.O.. Initial 7. ` 7
Final r - 4 ' '-7 1
Ternp. Initial
Final
Conductivity: Initial
Final i ? - �. j " -
Residual Chlorine:

JWAE7"ff 7 A=_ 4C7", Af~4_
Chemical and Physical Determinations
Page 2 of 2
Client: Lincointon NPDES#: NCO025496 Start Date: 4/12/16
Test Organism: Pimephales promelas End Date: 4/19/16
Day
Concentration: 11.0% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
pH: Initial
Final
D.O.: Initial t5.
A
Final
Ternp.: Initial
Final —7
Conductivity: Initial
9,61
3
Final
Residual Chlorine: z
— L.
Day
Concentration: 16.5% 0-1 1-2 2-3 3-4 4-5 5-6 6-7 Remarks
PH: Initial
le )
Final L4
D.O.: Initial Ci L
Final L1 -7 x
31/
Temp.: Initial f
I J
Final Ll 3
Conductivity: Initial
Al
�;-Final Yo
Residual Chlorine:
Day
I
Concentration: 22.0% 0-1 1-2 2-3 .3-4 4-5 5-6 6-7 Remarks
P14: Initial
Final
D.O.: Initial
Final
Temp.: Initial
Final Lj
2-
;,4
Conductivity: Initial F i
`Finals-7-.-
Residual Chlorine:
Day
1-2 -7 Remarks
Concentration: 100% 0 1 2-3 3-4 4-5— 5-6 6
pH:
Conductivity:
Residual Chlorine:
Hardness:
Alkalinity:

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lincointon NPDES#: NC0025496 Start Date: 4/12/16
Test Organism: Pimephales promelas End Date: 4/19116
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 i' ✓ (/ (�.
Rep B 0
Rep C 0
Rep D 0 I..
Concentration 5.50% 1
�J
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B 0
Rep C 0 ✓ C J
Rep D 0
Concentration 8.25%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
r
Rep A 0
Rep B 0
Rep C 0 y
Rep D 0 - °✓ 11 j
,y

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: Lincolnton NPDES#: NCO025496 Start Date: 4/12/16
Test Organism: Pimephales promelas End Date: 4/19/16
Concentration 11.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B 0
Rep C 0 ✓ i
Rep D 0
Concentration 16.5%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Or-
Rep
Rep A 0
Rep B 0
Rep C 0
P\
Rep D 0 i
Concentration 22.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 "
Rep B 0
f
Rep C 0
Rep D 0 .

ENVIRONMENTAL LABORATORIES
A Division of LVarer Techno/o Jv and Conrrofs. /nc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lincolnton Page: 1 of 2
NPDES #: NCO025496 Outfall:
Date of Test: 4/12/16
Initial weights taken on: - { - ;v- by: , 4-\ Organism: Pimephales promelas
Final weights taken on: �' �' E by:
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WTJORG. (mg) SURV%
A
775
i Z
A -2
5.50%
8.25%
f

(•s ENVIRONMENTAL LABORATORIES
� A O%vision of;Nater Technology and Controls. Inc.
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: Lincolnton Page: 2 of 2
NPDES #: NCO025496 Outfall:
Date of Test: 4/12/16
Initial weights taken on: �4- —1 by: I G-1 Organism: Plrnephaleg promelag
Final weights taken on: `f - :? I !;`: by: :7) ;
PAN WT. PAN + ORG. WT. OF ORG. MEAN
CONC REP (mg) WT. (mg) (mg) #ORG. WT./ORG. (mg) SURV%
y '2
11.0° e � '�� �� . : �1 %�
/o `
C ,c � �' Vii'i Lr �• ) 3
coo
16.5% B
i
D 71
1.
l
22.0%
B

MERITECH, INC. Meritech Sample ID#:
Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reed(aDmeritech-labs.com Web Site: www.meritech-labs.com
/ CLIENT INFORMATION
1
Client: (I, j``�_^ k iv L�l=1 LCU ixi-n—\ I5�\i(A/ PO#:
Contact Person: n i Tt� t � ��;;;-� �f �';`(`-i NPDES#: NC C C C-,
Address: UAL' L7�.'X (C t/ Phone:
City: L1'r'1 LCJ( d"\ fun Pipe#:
County: �''1, �.f.`r Y'1 State: 1V (___ Zip:
SAMPLE INFORMATION
Sample Site:
� —
Sample Type: ❑Grab Composite #of containers:
Sampling Time: Start Date: " 11 � Start Time: �l/ JS AMPM
r � LA
MfiEnd Date: '-��� ! EndTime: /� PM
l.:j.r;e ir:CS2...:��ses cC':ci;7c 't .�i� �:-: 4c t°J�r:.:"c`i: irk ,lii:r:rco-.:�•.... .-r��Sc.-r:'iG GCs-c`c�,:��ln'f C:l7:?08ir J.`CB.
t ack t,fs S=. lie cO:�"er CC!C— e!j,Ln fC... !t!e sa:,mp ie=s ;bs <C.0"C:w!pon reCG;-r Et elle ec�.Prc'iOC1'
Collector's Name: Print: �-Zi11`\�ir1—c`_a tIl a Signature: \ �
F
TOXICITY TEST INFORMATION
Test Required: ❑Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) [g Pimephales promelas (Fathead minnow) '
❑ Mysidopsis bahia (shrimp)
IWC: % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
l
Relinquished Time: 3•� �r
PM
Received by: ' v �f� Date: Time: II�i PM
3
Relinquished by: �'�� �%� Date: �:/..---�/ rh Time: 3 AM
- r -
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: . Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX jZMeritech Pick-up Delivered Other
Samples sh'pped on Frid_y must be FedEx and must be clearly Iabeied for Sa;ur zy delivery,140 SiGNILIURE REQUIRED"
SAMPLE RECEIVING(Laboratory Use Only)
Relinquished by: 1
Received by:j Date: Time: ~' AM r PM
Sample Temperatures(°C): / .` .L / l Sample Condition:
WI;iTE = Laboratory,copy YELLO'k = Client copy � .

MERITECH, INC. Mentech Sample ID#: `l `t I` �
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax: [phone]
Toxicity Supervisor email: mike.reed(cbmeritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: l t L-RI\ -(t't1�:' i • ` PO#:
Contact Person: NPDES#: NC
Address: 11�U $,i' y. (C I'/ Phone: 9 CLI, %_6
City: Lj 'n C_C in +0n Pipe#: (G}
County: `-}n CLL) d`l State: i y Zip:
SAMPLE INFORMATION
Sample Site:—'�
Sample Type: ❑Grab EXcomposite #of containers: lot 1 VII
Sampling Time: Start Date: ?,k^_ Start Time: i) T AM PM
End Date: y I It r)��i End Time: ( rj�l J M PM
iK
a C:.I'-.:.�C r•GrntJ:C CC:�_ ..:�..�- -t: 9 SE <:..�i-G
Collector's Name: Print: i lf1� C__ ��- Signature:
TOXICITY TEST INFORMATION
Test Required: ❑Chronic(7 days) Test Organism: ❑Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) NPimephales prornlelas (fathead minnow)
<<❑ Mysidopsis bahia (shrimp)
IWC: , % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: — ; Date: -� —/ - Time: 4 AMPM^'
Received by: 1 � �� �- �"-� �(' ` Date: l I 1 ( Time: I` , ( AMP-1 �
Relinquished by: ? .•� 1 c; ; ` ��` Date: 1 1`4,'t 1 Time: AM `M
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EX Meritech Pick-up ❑Delivered ❑ Other
Sampies shipped on Fricay must be FedEx and must be clearly labeied for Saturday Qelivenr. N'0 SIGNATURE REOUiFED
SAMPLE RECEIVING(Laboratory Use Only)
Relinquished by:
Received by' ; j w Date: `- j t ( Time: 1 AM�•J�M
Sample Temperatures(°C): C �` / / / Sample Condition: = `
;r>m it hF I E = Laborafc^,'cc^v t`ELL01 v =Client copy

MEIRITECH, INC. Meritech Sample 0#:
_ Bioassay Sample Chain of Custody
642 Tamco Rd,Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reedCilmeritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
fi
Client: `_�' ,i Lam' L-t r\C-0rl�1?yr� 1 4J 7 PO#:
Contact Person: b'�r1 i C•.��. �%t t1�!��1 ) NPCES#: NC ! �� ,)- S L G�
Address:
�c Phone:
f L_: � �"7
City Pipe#:
County: Ln C 0 !�` State: . Zip:
_ SAMPLE_ INFORMATION
Sample Site:
Sample Type: ❑Grab 1XComposite #of containers: !V W
Z
Sampling Time: Start Date: t' �/� Start Time: f?� tAM
PM
End Date: - S � 5 EndTime: I I (�S ) PM
r x r(ire(iirSe S.-'?(r�rc Cc,.-, sEnnp.2e before r fi(i:-. vG.�,.7aB�'c%}'i°% fh6 S�eTcP..'E CGRfaffne( 4'iu°f no air SG2Ce.
Foci:f.he SELF G CC^:ler CC:n.p efe!j'in ice. ISE Sc."r:is f-lus,be<6,0'.,;.pcn-J (ece.' cL I'E o^JO'Gzc *"
n.:•�t fi
Collector's Name: Print: - -fit t�'�� ;-c�' �'3i1� SignaiureE �
e
TOXICITY TEST INFORMATION
Test Required: ❑Chronic(7 days) Test Organism: ❑ Ceriodaphnia dubia (water flea)
❑ Acute(24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
IWC: % Test Concentrations(if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: Date: I��I L Time: !�/ �`} ,%AM' PM
Received b/ i i`�1//> c J - Data: '= Time: i, =ti ��'PM
Relinquished by:.-'" !' _ ,' ':' =� Date: Time: AM PM:
Received by: Date: Time: AM PM
Relinquished by: V Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature(°C):
Method of Shipment:❑ UPS ❑ Fed EXMeritech Pick-up ❑Delivered ❑ Other
�
"Samples shipped on Friday must be FedEx and must b6/clearly labeled for Saturday d0very,NO SIGNATURE REQUIRED
SAMPLE RECEIVING(Laboratory Use Only)
Relinquished by: L �•ti j
Received by: l� Date: : I ' i Time: AM PM
Sample Temperatures(°C): (,` 1~` l �"• I ! Sample Condition:
r =s V H`TE= Latc!atai y Copy `.`ELLC'N=Client CCF

Title: lincolnton - survival
File: lincolnsury Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
----------------------------------------------------------------------------
D = 0 . 0900
W = 0 .4806
Critical W = 0 . 8840 (alpha = 0 . 01 , N = 24)
W = 0 .9160 (alpha = 0 . 05 , N = 24)
------------------
----------------------------------------------------------
Data FAIL normality test (alpha = 0 . 01) . Try another transformation.
Warning - The first three homogeneity tests are sensitive to non-normality
and should not be performed with this data as is.

Title: lincolnton - survival
File: lincolnsury Transform: NO TRANSFORMATION
Hartley' s Test for Homogeneity of Variance
Bartlett ' s Test for Homogeneity of Variance
----------------------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance .
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless .
----------------------------------------------------------------------------

Title: lincolnton - survival
File: lincolnsury Transform: NO TRANSFORMATION
Steel ' s Many-One Rank Test - Ho: Control<Treatment
----------------------------------------------------------------------------
MEAN IN RANK CRIT. SIG
GROUP IDENTIFICATION ORIGINAL UNITS SUM VALUE DF 0.05
----- -------------------- ----------- ------- ------ ----- ---
1 control 1 . 0000
2 5 . 5 1 . 0000 18 . 00 10 . 00 4 . 00
3 8 .25 1 . 0000 18 . 00 10 . 00 4 . 00
4 11 1. 0000 18 . 00 10 . 00 4 . 00
5 16 . 5 0 . 8500 12 . 00 10 . 00 4 . 00
6 22 1 . 0000 18 . 00 10 . 00 4 . 00
----------------------------------------------------------------------------
Critical values are 1 tailed ( k = 5 )

Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Shapiro - Wilk' s Test for Normality
----------------------------------------------------------------------------
D = 0 . 1724
W = 0 . 9504
Critical W = 0 . 8840 (alpha = 0 . 01 , N = 24)
W = 0 . 9160 (alpha = 0 . 05 , N = 24)
----------------------------------------------------------------------------
Data PASS normality test (alpha = 0 . 01) . Continue analysis.

Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Bartlett ' s Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 5 . 1806 (p-value = 0 . 3942)
Data PASS B1 homogeneity test at 0 . 01 level . Continue analysis.
----------------------------------------------------------------------------
Critical B = 15 . 0863 (alpha = 0 . 01, df = 5)
= 11 . 0705 (alpha = 0 . 05, df = 5)

Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 5 0 .0686 0 . 0137 1.4337
Within (Error) 18 0 . 1724 0. 0096
----------------------------------------------------------------------------
Total 23 0 .2410
----------------------------------------------------------------------------
(p-value = 0.2599)
Critical F = 4 .2479 (alpha = 0 . 01, df = 5, 18)
= 2 . 7729 (alpha = 0 . 05, df = 5, 18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0 . 05)

Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 1 OF 2 . Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED MEAN CALCULATED IN SIG
GROUP IDENTIFICATION MEAN ORIGINAL UNITS T STAT 0 . 05
1 control 0 . 6558 0 . 6558
2 5. 5 0 . 5577 0 . 5577 1.4163
3 8 .25 0 . 6323 0 . 6323 0 .3396
4 11 0 .6110 0 . 6110 0 . 6467
5 16. 5 0 . 5000 0 . 5000 2 .2509
6 22 0 .6335 0 . 6335 0 .3216
----------------------------------------------------------------------------
Dunnett critical value = 2 .4100 (1 Tailed, alpha = 0. 05, df = 5, 18)
Title: lincolnton
File: lincolnton Transform: NO TRANSFORMATION
Dunnett ' s Test - TABLE 2 OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF MIN SIG DIFF % OF DIFFERENCE
GROUP IDENTIFICATION REPS (IN ORIG. UNITS) CONTROL FROM CONTROL
----- -------------------- ------- ---------------- ------- ------------
1 control 4
2 5 . 5 4 0 . 1668 25 . 4 0 . 0980
3 8 .25 4 0 . 1668 25 . 4 0 . 0235
4 11 4 0 . 1668 25 .4 0. 0448
5 16.5 4 0 . 1668 25 .4 0 .1558
6 22 4 0 . 1668 25 .4 0. 0223
----------------------------------------------------------------------------