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Home My WebLink AboutNC0024333_More Information (Received)_20241018Hazen October 15, 2024 RECEIVED OCT 18 2024 To: North Carolina Department of Environmental Quality V tCDEQ/D WR Division of Water Resources I /N PDES Street Address Raleigh, NC, 27699-1617 Attention: Mr. Mike Montebello Job: 32138-020: Monroe WWTP Expansion to 13.4 mgd We Are X Herewith Under Separate Cover Sent By: Blue Printer _ Hold for Pickup Sending You: Reports Tracings Prints Hand Delivered Mail Shop Drawings Specifications x Other x UPS FedEx No. Date Description 10115/2024 Monroe Wastewater Treatment Plant NPDES Permit Major Modification Application NC0024333 - Revision 10/15/2024 Monroe Wastewater Treatment Plant NPDES Permit Major Modification Application NC0024333 - Revision 10115/2024 Monroe Wastewater Treatment Plant NPDES Permit Major Modification Application NC0024333 - Revision 10/15/2024 Monroe Wastewater Treatment Plant NPDES Permit Major Modification Application NC0024333 — Revision USB Drive Remarks: Transmitted: Copies to: At Your Request For Your Information For Your Comments x For Your Approval Very truly yours, For Distribution Hazen and Sawyer Revise and Resubmit Other. File By: Mary E. Sadler, PE Hazen and Sawyer • 9101 Southern Pine Blvd. Suite 250 • Charlotte, INC 28273 • 704.357,3150 � x %Ity OI Monroe Water Resources monroenc.org • 704-282-4601 • PO Box 69, Monroe, NC 281 1 1 October 8, 2024 Mr. Mike Montebello Water Quality Permitting Section, NPDES Division of Water Resources North Carolina Department of Environmental Quality 1617 Mail Service Center Raleigh, NC, 27699-1617 Re: NPDES Permit Renewal and Major Modification Application (NC0024333) — Revisions City of Monroe Wastewater Treatment Plant City of Monroe, North Carolina Dear Mr. Montebello: An initial NPDES Permit Renewal and Major Modification Application was submitted in July 2024 for the City of Monroe Wastewater Treatment Plant (NC0024333). At the time of the submittal, a fourth second species whole effluent toxicity (WET) test had not been completed. Enclosed, you will find revisions to Table E of EPA Form 2A, as well as the additional WET test result. This will finalize all testing requirements for the NPDES Permit Application. Enclosed, you will find the revised attachments. The revisions include the following: • Application EPA Form 2A — Table E (revised) • Second Species Effluent Toxicity Testing — Fourth Test If you have any questions regarding any of the NPDES permit renewal application materials, please contact me at (704) 2824624 or sclark@monroenc.org. Sincerely, L,� cS e C"'A §Director of Water Resources Attachments cc: Amy Cook, PE, City of Monroe Kyle Edwards, City of Monroe Mike Parker, PE, Hazen and Sawyer Mary Sadler, PE, Hazen and Sawyer RE'e�v ao 0 C ? 1 g Z�24 14CUEQJU0R1N?OES EPA Identification Number NPDES Permit Number Facility Name Oulfali Number Form Approved 03105119 110000554873 NCO024333 City of Monroe WWTP 001 OMB No. 2040 0004 - TABLE E. EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results. Test Information Test Number 1 Test Number 2 Test Number 3 Test species Pimephales promelas Pimephales promelas Pimephales promelas Age at initiation of test In-house culture, <24-hr In-house culture, < 24-hr In-house culture, <24-hr Outfall number 001 001 001 Dale sample collected 03/01/2021 09/12/2022 03/04/2024 Date test started 03/02/2021 09/13/2022 03/05/2024 Duration 7 days (terminated 3/9/21) 7 days (terminated 9/20/22) 7 days (terminated 3/12/24) Toxicity Test Methods Test method number EPA 1000.0 EPA 1000.0 EPA 1000.0 Manual title EPA-821-R-02-013 EPA-821-R-02-013 EPA-821-R-02-013 Edition number and year of publication 4th edition, October 2002 4th edition, October 2002 4th edition, October 2002 Page number(s) Sam le Tvve Check one: ab T224-hoLr composite El Grab ❑� 24-hour composite ❑Grab 24-hour composite Sample Location Check one: ❑ Before Disinfection ❑ After Disinfection ❑� After Dechlorination ❑ Before Disinfection ❑ After Disinfection AfterDechlorinalion ❑ Before disinfection ❑ After disinfection ❑� Afterdechlorination Point in Treatment Process Describe the point in the treatment process at which the sample was collected for each test. Final effluent Final effluent Final effluent Toxicity Type Indicate for each test whether the test was performed to asses acute or chronic toxicity, or both. (Check one response.) ❑ Acute ❑✓ Chronic ❑ Both ❑ Acute ❑� Chronic ❑ Both ❑ Acute Chronic Moth EPA Form 3510-2A (Revised 3-19) Page 25 EPA Identification Nun 110000554873 iES Permit Number I Facility Name NCO024333 City of Monroe WWTP 001 Fan Approved 03105/19 OMB No. 2040-0004 The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results. Test Number Test Number 2 Test Number 3 Indicate the type of test performed. (cherkone ❑ Static response.) ❑✓ Static -renewal ❑ Flow -through Source of Dilution Water Indicate the source of dilution water. (Check ❑✓ Laboratory water one response.) ❑ Receiving water If laboratory water, specify type. moderately hard synthetic water If receiving water, specify source. NA LJ Statc 0 Static -renewal ❑ Flow -through R1 Laboratory water ❑ Receiving water moderately hard synthetic water NA Type of Dllutlon Water Indicate the type of dilution water. If salt ❑� Freshwater Freshwater ❑ Freshwater water, specify "natural" or type of artificial sea salts or brine used. ❑Sall water (specify)❑Saltwater (specify)❑Salt water (specify) Specify the percentage effluent L concentrations in the test series. 45%, 67.5%, 90%, 95%, 100% Control (0% effluent/100% dilution water) NA LJ Static ❑� Static -renewal ❑ Flow -through r❑ Laboratory water ❑ Receiving water moderately hard synthetic water NA 45%, 67.5%, 90%, 95%, 100% Control (0% effluent/100% dilution water) NA 45%,67.5%,90%,95%, 100% Control (0% effluent/100% dilution water) NA Check the parameters tested. ® pH ❑ Ammonia ® pH ❑ Ammonia ® pH ❑ Ammonia ❑ Salinity ® Dissolved oxygen ❑Salinity Dissolved oxygen ❑ Salinity ®Dissolved oxygen ® Temperature ® Temperature ® Temperature Acute Test Results Percent survival in 100% effluent NA % NA % NA % LC5o NA NA NA 95% confidence interval NA % NA % NA % Control percent survival NA % NA % NA 9/6 EPA Fan 3510-2A (Revised 3-19) Page 26 1 Identification Number NPDES Permit Number Facility Name Outfall Nu 110000554873 NCO024333 City of Monroe W WTP 001 EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY provides response space for one whole effluent toxicity sample. Copy the table to report additional test results. Test Number 1 ITest Number 2 NA I NA MA Form Approved 03105/19 OMB No. 2040-0004 Test Number 3 NOEC 1DD %, 100 % 100 % IC25 > 100 % > lo0 % > loo % Control percent survival 1GO % loo % 100 % Other (describe) NA NA NA Is reference toxicant data available? ❑✓ Yes ❑ No E Yes ❑ No ❑° Yes ❑ No Was reference toxicant lest within El Yes El No ❑� Yes ❑ No ❑✓ Yes ❑ No acceptable bounds? What dale was reference toxicant lest run 03/02/2021 09/13/2022 03/05/2024 (MMIDDNYYY)? Other (descdbe) 3-day sample collection 3-day sample collection 3-day sample collection EPA Form 3510-2A (Revised 3-19) Page 27 EPA IdenfificaUon Number NPDES Pend Number Facility Name Out1a11 Number Form Approved 03105119 110000554873 NCO024333 City of Monroe WWTP 001 OMB No. 20400004 TABLE E. EFFLUENT MONITORING FOR WHOLE EFFLUENT TOXICITY The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results. Test Information Test Number 4 Test Number Test Number Test species Pimephales promelas Age at initiation of test In-house culture, < 24-hr Outfall number 001 Date sample collected 09/09/2024 Date test started 09/10/2024 Duration 7 days (terminated 9/17124) Toxicity Test Methods Test method number EPA 1000.0 Manual title EPA-821-R-02-013 Edition number and year of publication 4th edition, October 2002 Page number(s) Sample Type Check one: ❑ Grab ❑� 24-hour composite ❑ Grab ❑ 24-hour composite ❑ Grab ❑ 24-hour composite Sample Location Check one: ❑ Before Disinfection ❑ After Disinfection ❑� After Dechlorination ❑ Before Disinfection ❑ After Disinfection ❑ AfterDechlorinalion ❑ Before disinfection ❑ After disinfection ❑ After dechlorination Point in Treatment Process Describe the point in the treatment process at which the sample was collected for each test. Final effluent Toxicity Type Indicate for each test whether the test was performed to asses acute or chronic toxicity, or both. (Check one response.) ❑ Acute 2 Chronic ❑ Both El Acute El Chronic ❑ Both ❑ Acute Chronic ❑ Both EPA Form 3510-2A (Revised 3.19) Page 25 EPA Identification Number NPOES Permit Number Facility Name Outfati Number Form Approved 03105119 110000554873 NCO024333 City of Monroe W WTP 001 OMB No. 2040-0004 TABLE E. EFFLUENT MONITORING O• WHOLE The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results. Test Number 4 Test Number Test Number Test Type Indicate the type of lest performed. (Check one ❑Static ❑ Static ❑ Static response.) ❑� Slalic-renewal ❑ Static -renewal ❑ Statc-renewal ❑ Flow -through ❑ Flow -through ❑ Flow -through Source of Dllullon Water Indicate the source of dilution water. (Check 2 Laboratory water ❑ Laboratory water ❑ Laboratory water one response.) ❑ Receiving water ❑Receiving water El Receiving water If laboratory water, specify type. moderately hard synthetic water If receiving water, specify source. NA Type of Dilution Water Indicate the type of dilution water. If salt ❑� Freshwater ❑ Fresh water ❑ Freshwater water, specify "natural' or type of artificial ❑ Salt water (specify) ❑ Salt water (specify) ❑ Saltwater (specify) sea salts or brine used. Percentage Effluent Used Specify the percentage effluent used for all 45%, a7.5%, 90%, 95%,100% concentrations in the test series. Control (0% effluent/100% dilution water) NA Parameters Tested Check the parameters tested. ® pH ❑ Ammonia ❑ pH ❑ Ammonia ❑ pH ❑ Ammonia ❑ Salinity ® Dissolved oxygen ❑ Salinity ❑ Dissolved oxygen ❑ Salinity ❑ Dissolved oxygen ® Temperature ❑ Temperature ❑ Temperature Acute Test Results Percent Survival In 100% effluent NA % % % LC50 NA 95% confidence interval NA % Control percent survival NA % % % EPA Form 3510-2A (Revised 3.19) Page 26 EPA Identification Number NPDEB Permit Number Facility Name Outfall Number 110000554873 NCOD24333 City of Monroe WV✓TP 001 MONITORINGTABLE E: EFFLUENT The table provides response space for one whole effluent toxicity sample. Copy the table to report additional test results. Form Approved 03105119 OMB No. 2040-0004 Test Number 4 Test Number Test Number Acute Test Results Continued Other (describe) NA Chronic Test Results NOEC 100 % % % IC25 > 100 % % %] Control percent survival too % % % Other (describe) NA Quality Control/Quality Assurance Is reference toxicant data available? Z Yes ❑ No ❑ Yes ❑ No ❑ Yes ❑ No Was reference toxicant test within acceptable bounds? ❑� Yes ❑ No ❑ Yes ❑ No ❑ Yes ❑ No What date was reference toxicant test run MMIDD ? 09/10/2024 Other (describe) 3-day sample collection EPA Form 3510-2A (Revised 349) Page 27 1 Environmental Testing Solutions, Inc. P. O. Box 7565 Asheville, NC 28802 Phone 9 (828) 350-9364 Bill To Monroe, City of Mr. Kyle Edwards PO Box 69 Monroe, NC 28110 I Date 9/23/2024 103041 s 0 0 o17 7 1-7 _ September 20, 2024 Mr. Kyle Ketchum City of Monroe 775 Treeway Drive Monroe, NC 28110 ETS Project Number: 19969 Test Start Date: September 10, 2024 Facility Tested: Monroe WWTP PO Box 7565 Asheville, NC 28802 Phone: (828)350-9364 Fax: (828)350-9368 Enclosed are toxicity test results for samples received by Environmental Testing Solutions Inc. Parameter Test Procedure, Method Number Result (ChV) Code THP6C Fathead minnow, Pimephales promelas, Larval Survival and Growth Chronic >100% EPA-821-R-02-013, Method 1000.0 All toxicity testing results required as part of your permit must be entered on the Effluent Discharge Monitoring Form (MR-1) for the month in which it was performed, using the parameter code THP6C. Additionally, the DMR Reporting Form AT-5 must be signed and e-mailed or mailed to the following address: ATForms.ATB(cDdeg. nc.gov North Carolina Division of Water Resources Water Sciences Section / Aquatic Toxicology Branch 1621 Mail Service Center Raleigh, NC 27699-1621 Please call if you have any questions concerning these results. jSincerely, Jim Sumner Laboratory Supervisor North Carolina Certificate Numbers: Biological Analyses: 037, Drinking Water: 37786, Wastewater: 600 South Carolina Certificate Number. Clean Water Act: 99053-001 a PO Box 7565 ° Asheville, NC 288028802 o Phone: (828) 350-9364 Fax: (828) 350-9368 Effluent Toxicity Report Form - Chronic Fathead Minnow Multi -Concentration Test Date: September 20, 2024 Facility: City of Monroe NPDES 9: NC 0024333 Pipe x: 001 County: Union Monroe V WiP Laboratory PerfooNngTest: Environmental Testing Solutions, Inc. Certificate x 037 Comm•no Signature of Operator in Responsible Charge (ORQ ORC Phone / E-mail: Pro aces: 19969 $¢naNrt a/fabpratary S...M3pr: "morec EW91607.Eamu"2 .EWelIO Test Organisms: Plmepholes promelas Or Mail Original to: North Carolina OMsion of Water Resources supplier Water Sciences Section/ Aquatic Toxicology Branch Imhouu CVlmra 1621 Mail Service Center Begin hetch: 09J)124 1355 Raleigh, INC 27699-1621 End hatch. 09.1624 Was Test Start Date: Se tember l0, 207A Control Organisms %Effluent 45x %Effluent G73x %Effluent 0 %Effluent 95% %Effluent Mh Water Control W Isul: Do Imgltl: Tem, I-Q: High Concentration PH(s ): DO Im910: Temp.I�CI: Replicate number 1 2 3 4 SUMNngnumber a/larvae 30 10 30 10 Odgin+l numberal lame 10 1 30 1 10 30 weighVedgimilmgRamel1 0.864 1 0.883 1 0.733 1 0.908 suMNn[numberaihme 30 i 30 i 10 30 Original numberorlame 1 10 10 10 Ii 10 WelghVodghW (mWmel 1 0.858 0.928 0.945 1 0.858 suMeln2 number of lame 1 30 10 i 30 i 10 odginalnumbvoftame I 10 1 10 1 10 1 30 werghttmighul(m&me) 1 0928 1 0.938 1 C.977 1 0.789 surviving number of lame 30 30 i 10 i 30 ontinal numberplhme 10 1 10 1 10 1 10 weishVongm.1lmgNmel 0.988 1 0.901 1 0.948 1 2.006 wr romimberoflame 10 10 i 10 i 10 odpnalmm,b atlame 1 30 1 10 1 10 1 10 WeighVonWu1lmgNmal1 0.996 1 0.970 1 1.010 1 1.017 wrvMnsnumber ollame1 10 30 i 1D 10 origmilnumberoflamae 1 10 1 10 1 10 30 Weight/oMwllmeamal I 1.032 1 0.956 1 1.DEA I 0.830 sunmllx) 300.0 AwnBa wtlmU1 0.947 Awnp vrt/1 0.347 nMNng (rod suMval rA) 100.0 Awngawt(mtl 1 0.872 sunhal lxl 100.0 Avengava(md 1 0908 Surmnilpn 100.0 AwraBewt(ma) I 0.961 survinll%1 100.0 Awngi,M(mt) 1 0.998 Survhall%1 300.0 Avenge vet (mgl 1 0.958 7.7s 12b 7A9 710 7a2 6.53 7.62 7.24 7.43 7.16 7.62 7.40 7.31 714 7.7 7.6 78 73 7A 55 7A 73 7.7 7.1 7.8 7.5 1.6 7.0 24.9 2S1 24.8 2U 24.8 25.1 24.8 25.1 24.825.2 24.9 23.2 248 251 5.93 G.7S 6.39 6.39 5.97 6.04 GAS SAS 6.47 6.49 6.64 6.75 682 fi.52 ].9 7.7 7.7 7.6 SO 6.2 7.9 7.6 8o 7.3 7.9 7.4 ).9 10 34.9 251 23A 1 251 1 25.0 25.0 I 25A 251 25.0 i 25.1 no 24.9 1 14.9 23.0 Sample Information Cotimui Mart data: Gnb: Compndu n: duredo AheaNry(mg/L C.COJ: Herdnev(mtACa000: COMu4tMty (IunheVanl: Toul nndual chlorim (mg/Ll: Sample Temp. at Receipt ('Cl: sainpiel Sample2 a24 010-Sa 24-h 24-h 14 12Ea 13 1�12-241: 130 140 "a 891 <O.Sa 40.100.10 0.7 13 1.7 Analyses Normal: Hem. Var. NOEL: LOEC: OV: Method: Overall Analysis: Result: PASS LOEC: >100% NOEC: 100% ChV: >100% DWR Report Farm AT-5 suM l Growth Yes Yet Yet Yet 100% 1003L >1003E >100% >1a0% >300f3 Wu.I I.P. Dmnen't sunhal Grmnh %Emuent Gitlin CskVlaud COtlnl Cdcubted 45% 2.410 4.555 673% 2.410 .1.340 90% 3.410 -2A98 1 95% 1410 -3.322 1" 2,420 -2A33 Page 1 of 5 _� FmlwnmmnrJTeaWpSalurM�Lc Chronic Whole Effluent Toxicity Test (EPA-821-R-02-013, Method 1000.0) Species: Pimephales promelas Client: Monroe WWTP County: Union NPDES #: NC 0024333 Outfall #: 001 Project #: 144W Dilution preparation: Dilution prep (%) 1 45 1 67.5 1 90 1 95 100 Sample was not aerated or treated unless otherwise noted on this foan. Sample was warmed to 25.021.0°C in a warm water bath and then diluted to the test concentrations with moderately hard synthetic water (MHSW). Effluent volume (mL) 450 67S 900 950 1000 Diluent volume (mL) 550 325 300 50 0 Total volume(ml.) 10()0 1000 1000 1000 10D0 Test organism information: Test information: Organism source: In-house culture Randomizing template: LZ Age: <24-hours old Incubator number and shelf location: l� Y Spawn date: 04-4h'V%i Artemio CHM number: CHM1294 Hatch dates and times: 04.aA-V1 vbSS -n Ato-Vl UADS Drying Information for weight determination: Date/Time in oven: .t1-V1 -Initial oven temperature: V O•C. Transfer vessel information: pH(S.U.)- •a Temperature(-Q---IIIL Date/Time out of oven: o%-% •t PASS •Flnai oven temperature: 40'C Average transfervolume (mL): <0.25 mL Total drying time: 1•t•11a0&S 06(rC Oven, Thermometer SN: 14-90585 Daily feeding and renewal information: Day Date Morning feeding Afternoon feeding Test initiation, renewal, or termination Sample number used MHSW batch used Time Analyst Time Analyst Time Analyst 0 09-10-24 0500 1430 l50 t`104110•� O'1aY•1�1A 1 09-11-24 OSoo llbo 0950 2 09-12-24osto l'LA O 4� %%Is -'00 3 09-13-24 OS to 'at, OF 4 09-14-24 6 ,Zoo 1 14 I 05.OR 1•I6 5 09-1S-24 b 1100 Oq j 6 09-16-24 7 09-17-24 aotSo Chemical analyses: Parameter Reporting Limit Method number Meter Serial number H 0.15.U. SM 4500.H+B-2021 Accumet AR20 93312452 Dissolved Oxygen (D.O.) 1.0 mg/L SM 45MO G-2021 YSI Model 52CE 09AI00271 Conductivity 14.9 µmhos/cm SM 2510 B-2021 Accumet AR20 93312452 Alkalinity 5.0 mg CaCO,/L SM 2320 B-2021 Accumet AR20 93312452 Hardness 5.0 mg CaCOs/L SM 2340 C-2021 Not applicable Not applicable Chlorine, Total Residual 0.1 mglL ORION 97-70-1977 Accumet AB250 92349123 Temperature 0.1°C SM2550B-2010 Digital Thermometer V1066 q 6 RS Control information: Acceptance criteria Summary of test endpoints: % Mortality: 17, 5 20% 7-day LC,(%) 7 100 Average weight per initial larvae: D• 8`•1-i NOEC (%) tool Average weight per surviving larvae: I 0•941 a0.25mg/larvae LOEC(%) 71OD 1. ChV (%) '.P 1007 ICU(%) >t007. SOP AT20-Revision 6-Exhibit AT20.3 . FmlmmmmrJTenhp Sdunona4c Species: Pimeohales aromelas Client: Monroe WINTP Survival and Growth Data Page 2 of 5 Date: 09-10-24 Day CONTROL 45% 67.5% A 8 C D E F G H I J K L 0 10 10 10 10 10 10 10 10 10 10 10 10 1 fp 10 l0 10 10 )0 10 10 /D ID l 0 1'p 2 10 10 10 10 10 1D 10 10 10 10 10 10 3 10 10 10 10 l0 I 10 10 ID 10 ID 10 4 l0 1 IO 10 10 ID 10 10 IO 10 10 10 I 5 10 10 ID 10 ID ►D 10 (D 1p Ip 10 ID 6 /o 10 10 !O 10 10 to 10 1D 1D 10 10 7 Ip 10 10 IO 10 t0 l0 10 10 10 (0 Io 'A Pan weight (-a)C OIZ Traymlorcode: 0 `� r� n `0 OD ^ ` aO n, ) Analyst �� Date: OA-a. ��. --\\y \v a I�/� `l• �' �` �• L1 \l,1• � �• b �• \^' 'B = Pan ♦ larvae weight (mg) Analyst. .61 IzAb t1.51 1.1.71 .29 12,0S 11.1% 22.'>a V{.QS t3t'� tyl.{p 20.03 Date: n414•19 C - larvae weight (mg) - B - A 6.&A d.t3 1.bz) 9.od &S& •U 9AS 8.59 Cluq-1& q,11 1.59 Analyst Weight per initial number of larvae (mg) = C / Initial number of larvae 0'. A, Analyst O W Ia. IS p 0' 0• 0' 0 0' 0 _� Average weight per Initial lame (mg) Percent number of reduction 0 gN� 0.8"1'L —3.07, 0.°10d from control(%) 'Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520. Comment codes: c = clear, d = dead, fg = fungus, it = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Comments: Envlmnmrn�al ANn I SNu W na E Species: Pimephalespromelas Client: Monroe WWTP Survival and Growth Data Page 3 of 5 Date: 09-10-24 Day 90% 95% 100% M N O P Q R 5 T U V W X 0 10 10 10 10 10 10 10 10 30 10 10 10 1 ID IO 10 10 [0 10 10 I to I p 10 10 2 ID 10 [p ID 10 lb ID 10 10 lb /0 /p 3 f0 10 l0 l0 1p ID l0 t0 l0 to l0 [o 4 10 10 10 ID 10 10 10 t0 l0 /0 10 10 5 10 10 10 10 10 (0 10 ID 10 10 to 10 6 (D 10 10 11) 10 to I ID 10 to 10 /0 ' ►u to tD to to Io (o l0 10 to t0 �� •A= Pan weight (mg) Tray color code: `1\p,/l\ 0 Analyst Date: •7. - y�`1 \�• \� ��' \1• �• ��• ti \� •B = Pan + larvae weight (mg) Analyst 25 Sj '11.58 Zt.93 'IASI V.S9 Tt. RA -t•3,g5 12•b7 2t.'1S -LA.S3 21.41 Date: nq•s4- C - larvae weight (mg) = B -A q.8F q.o1 q•y8 lo.o6 q•46 000 to.iD 10.11 lo.31- q.(.6 lo.o,4 8.10 Analyst -1A — Weight per initial number of larvae (mg) = C / Initial number of larvae Analyst: �• D• A �• p• U �• \• \• Q' \' D� Average weight per initial number of larvae (mg) Percent reduction q61 ,�j• 0 qq� _11•q 7• 0,q5& r from control N *Weight measurements performed using Cahn 28 Automatic Electrobalance, SN 41520. Comment codes: c = clear, d = dead, fg = fungus, k = killed, m = missing, sk = sick, sm = unusually small, Ig = unusually large, d&r = decanted and returned, w = wounded. Comments: e G 0 J enemmmviinm95WEeMK Quality Control: Verification of Data Entry, Calculations, and Statistical Analyses Chronic Whole Effluent Toxicity Test (EPA-921-R-02-013, Method 1000.0) Species: Pimephalespromelas r u We ..wr Tesility:t 2OZ4 Tezt start date: September 10, 2024 Septem September A*MeM� Project number: 19969 240910.07, 240912.10, 240914.02 Ice w�ru Sample numbers VAJ, GmNnLLnlel -YNI^MI W.W4/SunFYY Y•.nwlim/ [u114LW vlvM11M W.YY/Iran. Yws•MI1-ing) 00 ®w•rJbn.v lei ..=v=1 no 10 10 It" 20.% a." 0.864 am? 9.] 0064 0.80 9.2 NataDpOubh SO 10 14.30 23.13 8.83 0.%3 eastConeW 0.733100.0 10 10 2424 21.57 7.33 0.733 EA 10 10 14.21 23.29 9.08 0.%8 0.908 10 10 13.]1 22.29 8.58 0.858 . 43 158 0OB)l Mal 43 -3A 10 10 33." 23.05 9.2a 0.928 092E 10 30 1216 21-31 aAS Mass MUSloo.D 0AS8 30 10 13.72 22.30 8.58 ME% fi7.5% 10 10 159 24.85 918 0.928 0.900 9.0 0.928 TWA 0.%8 So -].2 1 30 10 13.96 23.24 9.38 0.938 0,938 It 10 10 13.63 23.40 9.]1 0.977 0.977 0.789 1 30 10 12.14 20.03 719 0.789 90% M 10 SO 15.65 25.53 9.88 09% 0.961 4.9 0.988 I%.a M%l 4.9 -13.4 N 10 10 13.S7 22.58 9.02 0.901 0.901 O 10 10 12AS 21.93 9Aa 0948 Osda P 10 SD 14.52 24.58 10.06 1.0436 1.006 95% Q ID 30 14.57 24.53 9.96 0,996 0.998 2.3 0.996 100.0 0.%a L3 -11.9 A ID 10 13.29 22.89 9,70 0.970 0970 2010 5 10 10 14.69 24,79 1010 1010 T 10 10 13AS 23.85 10.17 1.017 1.017 100X U 10 30 12.31 22.63 10.32 3032 0.958 9.3 1.032 IpOA 0.953 9.3 -13.1 V 10 30 1109 9.% 0.966 0.966 Laos V/ 10 30 14A9 US 24.53 1004 lZ 1t 30 10 14.17 1 22.47 8.30 1 0930 DA30 cethril of MSD. Minimum Slpnlfcant Offennca Demean'. MSD value: 0.1097 PMSD. Percent Minimum SlgmMnt Difference PMSD: ".a PMSOGamea4ereoftent PYcNlon. The PMSD IS uraminlmum pe¢an[dlllerenca betvrten Ne control and Vutmen[Wtunbe dedarM suNucath si[nlf n[InawMbeffluem mddrymst. sower PM6G Gouts determined by USEPA Seth percentile) a 12%. Uppar PMSD bound determined by USEPA (90th Percentile) a 30%. lower and upper PMSD bounds Were determined from the ION and Sour p.... mi e, «eDeatrely, of PMSD data from EPA's WET Inte,labantory VYteGlll, Shi (USEPA 2001a; USEPA, 30011h) USEPA 20010001b. Mel Bepore Int.mbornory VirNNOty Study of EPA Sho[bterm OvpNc aml Acute Mole Effluent Toddry Tut Methods, Volumes land 2-AppeMY. EPA-921 11a1A04 arW EPA-821d 01.005. US Environmental Protection Agency, Cincinnati, ON. amar Statistical Analyses 5 ! `1 -`, .i rmb.mmoltav,foNa.lr. a` V larval FYtr GYowm and Bur 1TOW My Glrowttl Sort Dale: 911 M024 T9etID: PpFRCR Sample ED: Monroe End Date: 9117=24 LnD ID: E Smoir. Testing Sol. Sample Type: DMR-Dlsobarge Mtndodng Report Sample Data: Pm10mI: FY.CHR-EPM21-11-02.013 Test Spedes: PP-penePDale3 piomelas Camnlenll: Cone.% 1 2 3 4 D-Control 0.8840 00830 0.7330 0.9030 45 U 0580 09280 0 "so 0.8580 GTS 09280 0.9mo 0.9770 0.7890 90 0.9880 0.9010 0.9460 1.0080 95 0.9900 0.9700 1.0100 1.0170 100 1A320 0.9660 1.0040 0.8300 'f Tnns/orm:Untnnafarmsd 1-Tall d Yoleale Ce'n4-14 Mean Nolan Mean Mkt Max CY% N tSYal Crmo4l 110190 Mean Nolan D-Control 0.8470 1A000 0.8470 0.7330 0.9080 9.222 4 0.9240 1.0000 45 02723 1.0298 0.8723 0.8450 0.9280 4.319 4 4.555 2.410 0.1097 0.9240 1.0000 87.5 0.9080 1.0720 0.9080 0.7890 09770 9.042 4 -1.340 2410 0.1097 0.9240 10000 90 0..9608 t.1343 0.9608 0.9010 1.0060 4.853 4 -2.498 2.410 0.1097 0.9240 10000 95 0.WS3 1.1766 09983 0.9700 1.0170 2.079 4 41.322 2410 0.1097 0.9240 10000 100 0.9580 1.1311 0.9580 0.8300 1.0320 9.344 4 -2.438 2.410 0.1097 0.9240 10000 INanY T.ab SY9305 Skew lout SDapim-V91Ks Tesl lndimYs normal dlsmDWon (p>001) 0.89305 0..8oa1 0.eB4 -1.048 0.51382 BamaRS Teal irWicates equal wdances(p-0.26) 6.50955 15.0863 eY Teal1-bi,'.a5 NC150 LDEC C0Y T9 MSDu MSDp MSE MSE "rob d1 Dunnetfs Test 100 >100 1 0.10973 0,12958 0.01351 0.00415 0.02872 5.18 Treatments we D-Conent DdarpoYlbn (200 Hasamples) �Pobrl % SD 96X pIJroar Sk.w IC05 >100 C10 100 IC15 >100 td IC20 >100 o.s IC25 >100 0.8 IC40 >100 0.7 ICSO >100 I 0.0 . 05 0.4 „ oa 8 02 0.1 oa ._ _ .oz 413 0 50 led 150 Dea. % Oov.-Raspome Plol 13 t 0.0 0.051ew1 ofslg of clgnlfleance 0.8 8 0.. 0.2 0 J 8 IS 8 I 0 Page 4 of 5 , Emimmunr.ITeNnp Stivaona Nc Species: Pimeoholes aromelas Client: Monroe WWTP Date: 09.10-24 Daily Chemistry: Temperatures performed at the time of test initiation, renewal or termination by the analyst Identified in the Daily Renewal Information table located an Page 1. Alkalinity, hardness and chlorine (total residual) performed by the analyst Identified on the bench sheet specific for each analysis and transcribed to this bench sheet. Analyst Day (Analyst Identified for each day, performed pH, D.O. and conductivity measurements only.) 0 i i m Concentration Parameter C1: Koss~, W,iiiesm. livil. -1 iJ5?'.xza' - PH(S.U.) .1 ��. 9 7w b.A3 Dissolved oxygen(mg/L) 9 .> CONTROL, Corlductivi tY (tu^hos/an) Zq Q '6'�"i°0%; �er aare�0 3130 4s`•'<4ACAW 31 MHSW Alkalinity (mg CaCO3/LI 6.4 ', v; '+ 7 Haniness(mg CaCOJL) i1 -'W-e. f.�ri1'a'�a Temperature('[) l•49 15-1 u•t i'1. f ti5-\ PH (s.U.) (e.9 l z fl 1 7. Dissolved oaygen(mg/L) 1.-\ Conductivity (pmnas/Dn) ,;.rs-.- Temperature('[) 1`\-& •<S.0-., VAA •\$.-L. UA Y)\.S. PH(s.U.) (..SR 7 6 t e.50 Dimmed aaygen(mg/L) 1-$ ,(O 11 .2_ 67.5% Conductivity(pmhos/on) S4 �'i�'na�". .: ; .trl- V Temperature(-C) liar• (• ZS-0 VIA 1,5.1—• 1`I.0% •LS.1 PH(S.U.) .•i.¢t G G.1 G. a �n.I(a Dissolved oxygen(mg(L) l.0 ,1 ^ -t 90% Conductivity(ILmhos/tm) Temperature(-C) l.S .j 1S •O S•o li. U •1 PH(S.U.) b.0 6• - Sa G•S ls1 Dissclvedoaygen(mg/Ll -IA •� 2 9S% ConducWity(Wnhne/an) 1Jg ;. 'L:'S.: �00 r'•�`'!Y:-: S;r ::.it-:?:(: Temperature K) •ty.$ lS-1 1S•0 lS.C) '\S.0 is.-L PH(s.U.) S -IM . 3 . ?9 L. tl Dissolved oxygen(mgA) 1- ,� /4.10 Conductivity (lun_11lal 18 +:-114`it�: ):.-. 'L ';AS�66'a ig q 100% Allanmty lmg wcoJU 14 11 tpl p.MI—VA fib li 1t`^Y`ftRw Hardness(mg CaCOs/U 130 4tYv �x'''+Ji aAXAw's`. Raw" Iy0 ?lwn*U� Chlorine(mg/L) Go.ID F.r`(sV"':" - 0.LD `. Temperature I-Cl 1 ZS • t l.S .0 lS • 15. 0 't55. 0 Initial Final Initial I Final Initial Final SOP AT20-Revision 6-Exhibit AT20.3 P !r- (�! Page 5 of 5 mwenm.mdrmlrgse�Wena�,u. Species: Pirrleohatesoromelas Client: Monroe WWTP Date: 09-1O-24 Analyst Day (Analyst Identified for each day, performed pH, D.O. and conductivity measurements only.) 3 4 5 6 Llk 95c, L. 6.4 Concentration Parameter ®� e PHltu., , 2 -4-43 4. ly Ttq 1 71r 7, D+nPbmdPm.�(nanl 15 9.1 I'l ,4 CONTROL, uw-m r(Wm Af-N 0 O MHSW nD,smr,(mt c.myil :re 1. xmePen (mtr w,N &A Temp W.('Q • .. re not. 4 - L- PH(SLL) Lo, :.t><i .; }.Oo 3. a 7.3 3 7 �Ft{• 0 mvoAred Parten4RA) .to 5 4.0 I'l 7, %.vi 1A -1.\ 45% cma.manlw./.m, �j' SL M ilia 5 �L T�m %A. fr- Za. U. � PH lsu, U110 4.B 4 I.17o b. +F ,08 Z16 2- 6.95 DlmMdorytmlmtA) ,b 73.V RA 1 �'.�/ �1.a .'L 67.s% co.aw RYG W-) , Lois 1 499 rmP•..P..m v1. Y.t- .a 'b' .o 'L4. �S.o lV.& PH n.U.) t4- (o.td9 (o.LP} (. V4 .9 7 S .70 ouoM1eaoattm(mtA) �A.- 90% tm,M„mary twRm) '3 �24 G'tl TrmP Vcl U ZS. -L.. 1�1.q zd.. o ti�1. Zs. o '\.d.4 PH(suJ 11.54 to.56 .5 4. 7 .96 DivW.ee ogtenlmtA, •a. i•.2 IA 1• -1A 1.L 9s% CPPdPeMnlPmhm/n*> d �6 r� rQ %4. ZS•o 19. %S.o 'LS•D %16.% -Lq.lk PH(su.) .Pi$ to.4 IP-43 10.1,04 .7 6• PL A-511- 0 [PPdPetdglPmhP>rcm) l ` `d 9 tl its -i `� � 100% ADS* timgC ,A) t3 HUMts, ImtheDyl) ® ® 1�10 � - wo.wlmtAl tO.ro T.mPa.m.m 'IS•O tL i•' ZS.o -1.5. -LS.o ILIA. at 14.9 15.O Initial Final Initial Final Inal Final Initial Final SOP AT2O-Revision 6-Exhibit AT2O.3 • 0 351 Depot Street i Whole Effluent Toxicity Asheville, NC 28801 Chain -of -Custody Form Phone: (828) 350-9364 .� Envlronmental Testing Solution;Inc Fax: (828)350-9368 Client: City of Monroe NPDESII: NCO024333 outfall: 001 County: Union Facility: MonroeWWTP Purchase order: 140071 Species: P/mephales promelas Effluent dilution: 45, 67.5, 90, 95, 100% Test type: Full Range Chronic Parametercode: THP6C mrormanon: [to oe Grab sample:�J l.�l.' JVI(U111/`( Startdate: " Time: Composite sa le: f �� En I dater L4 Time: 1 End date: C C-/ Time: Flow proportional (d): or If samples/hour: Chilled during collection (yes/no)? If chilled, specify temperature rQ • Q Sample location: bb\ Volume collected for testing: 2- Cr -4- - 1. Number of containers filled for testivi X^ 3 Method of transport to laboratory: FPA2Y- Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) BYSIGNING BELOW, I CERTIFY THAT THE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM ARE ACCURATE. Samole collected bv. IIQ `tlocl(Zy PNI L(nldn Un..M umn Relinquished by: I.L '11"'w 16 i v Lctv�P/s' ( (Z GL�I t �t l 1012 Sample receipt Information: (to be completed by ETS personnel) Relinauiahed to M 6 .. Ram /O�ly rant vovrvn Custody seals intact (V)T Y. No N.MwA Samples received In good condition (V)sT.�/��f Un Q� FedEc/UPS cracking number. W " rt 7 1 JNa Comments: o...t.»n 1..,. I CI 1 0ti.�q.2,� YMf Ypl+[un "I ....wen... M+- /j� I/L /Oil y "t Sgo+M. .1..M. . Sample temperature upon receipt at ETS: Projectnumber: IMA Sample number: 1y0j 10. n Environmental Tests g 5olutions.Inc Client: City of Monroe Facility: Monroe WWTP Whole Effluent Toxicity Chain -of -Custody Form 351 Depot Street Asheville, NC 28801 Phone: (828) 350-9364 Fax: (828) 350-9368 NPDESN: NC0024333 Outfall: 001 county: Union Purchase order: 140071 Species: Ceriodaphnia dubia Effluent dilution: 90% Testtype: Pass/Fall Chronic Parametercode: TGP30 imormaaon: tto oe Grab sample: Start date: Time: Composite sam le: Start date: Q 7it•l Time: loay End date: 12, 1 It IZ" Time: L17J_) Flow proportional (4): or # samples/hour: Chilled during collection(yes/no)7 \A�� If chilled, specify temperature(DC): ��•'() Sample location: UVt Volume collected for testing: Number of containers filled for testing: --t: Method of transport to laboratory: Comments: Triple rinse sample container with sample before filling. Completely fill the sample container with no air space. Pack the sample container completely in ice. The sample must be < 6.0DC upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) BYSIGNING BELOW, f CERTIFY THATTHE PERMIT AND TEST REQUIREMENTS IDENTIFIED ON THIS FORM AREACCURATE. Sample collected by: uklutiwin U1 I(I Z4 �„ prn.M. Dn.uaivn. t,�tindz-t Relinquished by. Received by; Lt Vi 'h G 1 AM ASV✓V 06 LI y al Rl.(IL'� n. Spm,. One.M Wne "Fl•V rMl syn,tun DnvwM1v 1 Sample receipt Information: (to be completed by ETS personnel) Relinquished to US by: ma �` psn.mr. o.u.mlM. Custody seals Intact(v)T En Y. xa xa used Samples received in good condition (V)T FedEx/UPS tracking number. lY Jeq "t 1) Z RN TT Received at US bv: tk- O056 q.luy Sample temperature upon receipt at ETS: Project number: (9 9109 Sample number: Ati I[A IZ•I 0 Comments: 351 Depot Street Whole Effluent Toxicity Asheville, NC 28801 0117_17 Chain -of -Custody Form Phone: (828) 350-9364 Environmental Testing Solutions Inc. Fax: (828) 350-9368 Client: City of Monroe NPOES lt: NCO024333 outfall: 001 County: Union Facility: Monroe WVVTP Purchase order: 140071 Species: PimephaleS promelos Effluent dilution: 45, 67.5, 90, 95, 100% Test type: Full Range Chronic Parametercode: THP6C imormauon: Ito oe Grab sample: Sample location: Ci Q I Vr/r Start date: Time: Volume collected for testing: t S L Composite sam$: Number of containers filled for testing: Start date: �11 Z f 2u} Time: 101 Z Method of transport to laboratory: 6% End date: 13 12L-f Time: � Comments: Flow proportional(,r): or ksa pl s/hour: Chilled during collection (yes/no)? If chilled, specify temperature (°C):UG, Triple rinse sample container with sample before filling. Completely fill the sample container with no airspace. Pack the sample container completely in ice. The sample must be < 6.0°C upon receipt at the laboratory. Sample custody: (to be completed by sample collector and facility personnel) BYSIGNING BELOW,1 CERTIFY THAT THE PERMIT AND TEST REQUIREMEP Samole collected br. �;>trn u �6Zw �113124 I mnn Rellmulshed hw.. L n- 1f C'Gtr LLCM of u mm vm.un un...dun. Sample receipt information: (to be completed by ETS personnel) Rellnnuished eo FTS hr. oti-II444 Rqb� 1 1 1oi7 .m. Nm.on. vn..1..... Custody seals intact()? = Y. No Not m.d Samples received In good condition (J)? FedEx/L1PS tracking number: Comments: Received bv: ON THIS FORM ARE ACCURATE. C X D 1.f57 rmt se.+m Received at ETS bv: (4zy 1� Lk- ols MN en..ndu. Sample temperature upon receipt at ETS: Project number:1%06I Sample number: 1VOD(IVuf1L Pimephales promelas 7, Chronic Reference Toxicant Control Chart 7 0.9 0.8 0.7 0.6 Cd Y 0.5 bA V Uj 1.2 A 13 n 1.0 0.8 0.6 0.4 ^°°m••�r,°�=•°°°• Source: In-house Culture Control Limits (± 2 Standard Deviations) ------------- - — — — — — — — — — — — 0 — — — — — — •-- Warning Limits O�'2> OO 1�1; 0�1; 1%Is 'Ls Is p91> O� 13 Al as "'V' p9'Ls 06.20 O51A �z% 0'j.1u 00.1 A0!, Oy.9.o 109•N p3' o°' 05 p6. oi' OB 09 ,a 1ti ,tti 11 oy p1. 03. �. 09. 06. Oi 06. 09. Test date • 7-day IC, = 25% inhibition concentration. An estimation of the potassium chloride concentration which would cause a 25% reduction in Pimephales growth (calculated using ToxCalc). Central Tendency (mean logarithmic IC25 converted to anti -Logarithmic values) — — — Control Limits (mean logarithmic IC,, t 2 standard deviations converted to anti -logarithmic values) —. •—• Laboratory Warning Limits (mean logarithmic IC, ± 2 coefficent of variations converted to anti -logarithmic values) • • • •..... USEPA Warning Limits (mean logarithmic IC35±S,rs converted to anti -logarithmic values, 5MS = 750 percentile of Us reported nationally by USEPA)