NC0082376_Report_20060227

HAZEN AND SAWYER
Environmental Engineers a Scientists
February 27, 2006
North Carolina Division of Water Quality
NPDES Unit (East), Surface Water Protection Section
1617 Mail Service Center
Raleigh, North Carolina 27699-1617
Re: City of Raleigh
E.M. Johnson WTP
NPDES Permit No. NCO082376
Dear Sirs:
Hazen and Sawyer, P.C.
4011 WestChase Blvd.
Suite 500
Raleigh, NC 27607
[phone]
[phone] (Fax)
Enclosed is one copy of a report on the Flow Characterization Study for Outfall 002 at
the City of Raleigh E.M. Johnson Water Treatment Plant (WTP). This report is being
submitted on behalf of the City of Raleigh to meet the requirements of Item A.(6.) of
the NPDES permit for the E.M. Johnson WTP.
Please let us know if you have any comments on this report.
Very truly yours,
HAZEN AND SAWYER, P.C.
���yAW
Robert A. Berndt, P.E.
Senior Associate
RAB/mdb
cc: Environmental Sciences Section/ Aquatic Toxicology Unit w/encl
Surface Water Protection, Raleigh Regional Office w/encl.
Mr. Dale Crisp w/encl.
Mr. Bryan Hamilton w/encl.
Mr. Larry McMillan w/encl.
r r-.1 -) 200I6
NPDES 02,27.06 Ih.
New York, NY • Philadelphia. PA • Detroit, MI • Raleigh. NO • Charlotte, NO • Greensboro, NO • Atlanta, GA • Fairfax, VA • Baltimore, M0 • Hollywood. FL • Boca Raton, FL • Fort Plems, FL • Sarasota, FL • Miami, FL

am
E.M. JOHNSON WATER TREATMENT PLANT
FLOW CHARACTERIZATION STUDY
-
a' Introduction/Background
The City of Raleigh has been issued an NPDES permit (No. NC0082376), effective on
August 1, 2005, for the discharge of wastewater from the E.M. Johnson Water Treatment
Plant (WTP) into waters of the Neuse River Basin. As a requirement of Section A.(6.) of the
permit, a flow characterization study of Outfall 002 is required to be submitted to the N.C.
Department of Environment and Natural Resources, Division of Water Quality, NPDES Unit.
The E.M. Johnson WTP's main wastewater discharge is produced from the treatment
processes for the residuals from the drinking water treatment process, and is discharged
FM from Outfall 002 into an unnamed tributary to Honeycutt Creek, which drains to an arm of
Falls Lake. These receiving waters are classified as WS-IV-NSW-CA, which applies to the
critical area of water supply waters in moderately to highly developed watersheds. Nutrient
Sensitive Waters (NSW) is a classification for waters requiring further nutrient management
strategies to control nutrient (nitrogen and/or phosphorus) inputs.
Im
To meet the permit requirements for this study, the flow characterization study for Outfall
002 consisted of a single, continuous, 7-day monitoring event for the effluent. A total of four
parameters, flow, conductivity, settleable solids, and acute toxicity, were monitored at
different monitoring frequencies. The selected study period, from 8:00 AM, November 14,
2005 through 8:00 AM, November 21, 2005, is representative of facility operations and
discharge and coincided with quarterly chronic toxicity monitoring of Outfall 002.
Methods
The location for all samples for the flow characterization study was at the existing effluent
monitoring point for Outfall 002. Effluent flow was monitored continuously using the effluent
~" Parshall flume and recorded at one -quarter hour intervals during this sampling event.
Conductivity was monitored continuously using a conductivity probe, which was set up to
collect data once an hour over the 7-day period.
Settleable solids were also monitored at an hourly frequency. These were effluent grab
MR samples and were stored in a refrigerator at 4 degrees Celsius and then analyzed in a lab
within 48 hours. The analysis of settleable solids was in accordance with the 18th edition of
MR
1

Standard Methods for the Examination of Water and Wastewater, Section 2540F,
Volumetric Method. This technique utilizes an Imhoff cone, which is a transparent cone -
shaped container that holds one liter of liquid. The volume of settleable solids can be read
off the cone in the units of mUL.
..
Acute toxicity of the effluent, performed as a 48-hour static test with Ceriodaphnia dubia
(Parameter Code TAA313), was required to be conducted with one grab sample taken during
a representative peak flow period during the 7-day monitoring period. This test determines am
the lethal concentration (LC) of the effluent required to kill off 50 percent of the test subjects,
in this case Ceriodaphnia dubia, a freshwater invertebrate commonly known as the water MR
flea, within 48 hours. The acute toxicity test of the E.M. Johnson WTP effluent was
conducted with 20 organisms, 5 per replicate (Replicates A through D) at five different
effluent concentrations, plus a control. This control was examined to show the reliability of
the test, and must yield a 90 percent or greater survival of the test species. The
temperature was kept at a constant 25 degrees Celsius (plus or minus 1 degree), while the ..
dissolved oxygen, pH, and conductivity were required to be within acceptable limits. In
addition, total residual chlorine was also measured initially and reported (EPA, 2002). The
acute toxicity testing was performed by Meritech, Inc., Reidsville, NC. A copy of the acute
toxicity report (including the original DWQ Form AT-1) is attached.
No
Results
ON
During the 7-day flow characterization study of Outfall 002, effluent flows ranged from 1.05
to 14.88 mgd, with an average flow rate of 3.16 mgd. The conductivity averaged 203.38
/rmhos/cm, and ranged from a minimum of 4.32 ymhos/cm to a maximum of 219.95
/umhos/cm. Settleable solids were below the detection limit (0.1 mUL) for all samples taken
except one, a duplicate on November 15, 2005. Because the initial sample taken did not
^
produce any settleable solids and there were no detections in any of the other samples
during this test period, this single deviation is most likely a reporting error. Table 1 shows
the data collected for the study for flow, conductivity, and settleable solids. Figure 1 shows
the flow and conductivity data over the 7-day period. The results show that conductivity
was relatively constant over the 7-day period.
The report for the acute toxicity test is included in Appendix A. The results of the acute
toxicity test showed an LC50 at an effluent concentration of 96.6 percent. The results for
chronic toxicity tests conducted during the same time as the acute toxicity test are also
included in Appendix A. Two different labs performed chronic toxicity tests, Meritech and
Pace Analytical. For the chronic toxicity test conducted by Meritech, residual chlorine was
initially calculated at 2.54 mg/L, which should have yielded a 100 percent mortality of the
..
2
P,

a"
.,
0"
F"
higher dilution test organisms (see memo). When this did not occur, the effluent was
retested for chlorine, producing similar results. It was discovered that the detected residual
chlorine was due to the existence of suspended solids in the sample, and upon their
removal using a No. 3 Whatman filter paper, no residual chlorine was detected. For the
chronic toxicity tests, a chronic value of 94.9 percent was reported by Meritech and a
chronic value of >100 percent was reported by Pace Analytical. The E.M. Johnson WTP's
NPDES permit requires quarterly chronic toxicity monitoring to be conducted at an instream
waste concentration of 90 percent.
References
1. American Public Health Association, American Water Works Association, Water
Environment Federation, Standard Methods for the Examination of Water and
Wastewater. 18th Edition. 1999.
2. U.S. Environmental Protection Agency, "Methods for Measuring the Acute Toxicity of
Effluents and Receiving Waters to Freshwater and Marine Organisms," EPA/600/4-
90/027F, Fifth Edition. October 2002.
3

TABLE 1
Flow Characterization Study
November 14, 2005
Time
Flow (mgd)
Conductivity
mhos/cm
Settleable Solids (ml/L)
8:00
2.04
201.10
<0.1
8:15
2.67
8:30
2.50
8:45
1.80
9:00
1.69
204.48
<0.1
9:15
1.92
9:30
1.66
9:45
2.00
10:00
2.83
202.45
<0.1
10:15
2.87
10:30
2.45
10:45
2.25
11:00
1.69
205.60
<0.1
11:15
1.55
11:30
2.24
11:45
2.19
12:00
2.22
207.08
<0.1
12:15
2.75
12:30
3.17
12:45
2.19
13:00
1.91
205.05
<0.1
13:15
2.32
13:30
2.51
13:45
2.74
14:00
2.70
205.36
<0.1
14:15
2.27
14:30
1.68
14:45
1.88
15:00
2.65
206.17
<0.1
15:15
2.88
15:30
2.45
15:45
2.66
16:00
2.32
207.27
<0.1
16:15
2.15
16:30
2.28
16:45
1.99
17:00
2.56
207.83
<0.1
17:15
2.30
17:30
3.19
17:45
2.50
4
Time
Flow (mgd)
Conductivity
mhos/cm
Settleable Solids (mill-)
18:00
2.29
209.64
<0.1
18:15
2.14
18:30
1.87
18:45
2.39
19:00
2.03
211.16
<0.1
19:15
2.98
19:30
2.77
19:45
2.68
20:00
2.65
212.70
<0.1
20:15
2.12
20:30
2.54
20:45
2.02
21:00
2.52
216.37
<0.1
21:15
2.33
21:30
2.55
21:45
2.73
22:00
2.34
213.04
<0.1
22:15
12.07
22:30
14.07
22:45
9.35
23:00
6.85
4.32
<0.1
23:15
6.80
23:30
4.61
23:45
1 2.90
1 l 1 1 1 1 1 1 7

1 I 1 l l I l
TABLE 1 (continued)
Flow Characterization Study
November 15, 2005
Time
Flow (mgd)
Conductivity
(urnhos1cm)
Settleable Solids (ml/L)
0:00
2.16
212.57
<0.1
0:15
2.01
0:30 1
1.74
0:45
1.99
1:00
2.17
4.44
<0.1
1:15
2.64
1:30
2.63
1:45
2.43
2:00
2.39
212.94
<0.1
2:15
1.95
2:30
2.41
2:45
2.31
3:00
2.44
218.72
<0.1
3:15
2.41
3:30
2.05
3:45
2.83
4:00
2.11
214.01
<0.1
4:15
2.29
4:30
2.19
4:45
2.41
5:00
2.44
213.49
<0.1
5:15
2.23
5:30
2.73
5:45
2.13
6:00
2.39
157.22
<0.1
6:15
2.43
6:30
2.80
6:45
2.53
7:00
2.17
212.64
<0.1
7:15
2.72
7:30
2.31
7:45
3.08
8:00
2.77
217.10
<0.1
8:15
2.62
8:30
2.29
8:45
2.25
9:00
2.85
214.28
<0.1
9:15
10.75
9:30
14.16
9:45
7.74
10:00
5.94
212.76
<0.1
10:15
4.43
10:30
2.57
10:45
2.39
11:00
2.10
212.77
<0.1
11:15
2.95
11:30
2.51
11:45
2.00
-uupiicate at time lv:uu peiaea a result or >u.i MUD
Time
Flow (mgd)
Conductivity
(Pmhos/cm
Settleable Solids (mVL)
12:00
1.74
212.33
<0.1
12:15
1.61
12:30
1.81
12:45
1.75
13:00
2.85
211.96
<0.1
13:15
2.93
13:30
2.75
13:45
2.04
14:00
1.82
214.80
<0.1
14:15
2.50
14:30
2.22
14:45
3.09
15:00
3.50
212.78
<0.1
15:15
2.90
15:30
2.07
15:45
1.67
16:00
1.64
212.49
<0.1
16:15
2.51
16:30
2.59
16:45
2.98
17:00
3.28
215.21
<0.1
17:15
2.44
17:30
1.97
17:45
2.47
18:00
2.11
211.14
<0.1
18:15
2.17
18:30
3.08
18:45
2.95
19:00
2.77
210.61
<0.1'
19:15
2.68
19:30
2.31
19:45
1.97
20:00
2.02
209.40
<0.1
20:15
2.47
20.30
2.68
20:45
2.70
21:00
2.35
209.03
<0.1
21:15
2.01
21:30
1.77
21:45
1.71
22:00
2.37
208.94
<0.1
22:15
2.64
22:30
2.23
22:45
2.17
23:00
1.97
209.68
<0.1
23:15
1.77
23:30
1.95
23:45
1.67
5

TABLE 1 (continued)
Flow Characterization Study
November 16, 2005
Time
Flow (mgd)
Conductivity
(pmhoalcm)
Settleable Solids (ml/L)
0:00
2.21
210.67
<0.1
0:15
2.63
0:30
8.53
0:45
11.96
1:00
7.05
208.21
<0.1
1:15
6.15
1:30
11.65
1:45
8.80
2:00
5.94
210.88
<0.1
2:15
6.64
2:30
12.77
2:45
7.17
3:00
2.80
207.23
<0.1
3:15
2.21
3:30
2.30
3:45
2.56
4:00
2.41
215.22
<0.1
4:15
2.18
4:30
1.86
4:45
1.88
5:00
1.82
209.00
<0.1
5:15
2.26
5:30
2.71
5:45
2.74
6:00
1.94
208.45
<0.1
6:15
1.86
6:30
2.09
6:45
1.99
7:00
2.19
208.82
<0.1
7:15
2.49
7:30
2.72
7:45
2.43
8:00
2.60
209.24
<0.1
8:15
1.88
8:30
1.53
8:45
2.03
9:00
2.33
210.42
<0.1
9:15
2.48
9:30
2.78
9:45
2.23
10:00
2.01
207.34
<0.1
10:15
2.21
10:30
2.41
10:45
2.74
11:00
2.88
213.13
<0.1
11:15
3.06
11:30
2.38
11:45
2.14
Time
Flow (mgdd
Conductivity
hos/cm
Settleable Solids (ml/L)
12:00
2.82
207.46
<0.1
12:15
2.79
12:30
2.98
12:45
2.71
13:00
3.01
206.98
<0.1
13:15
2.58
13:30
2.66
13:45
3.18
14:00
2.54
206.68
<0.1
14:15
2.25
14:30
2.00
14:45
2.33
15:00
2.85
209.04
<0.1
15:15
2.89
15:30
2.86
15:45
1.98
16:00
1.86
204.82
<0.1
16:15
2.12
16:30
2.85
16:45
2.96
17:00
2.27
204.08
<0.1
17.15
1.89
17:30
1.85
17:45
2.17
18:00
2.39
204.04
<0.1
18:15
2.67
18:30
2.62
18:45
2.12
19:00
1.87
205.14
<0.1
19:15
2.59
19:30
11.97
19:45
14.60
20:00
6.83
207.75
<0.1
20:15
5.75
20:30
4.87
20:45
3.09
21:00
3.05
210.67
<0.1
21:15
2.49
21:30
2.23
21:45
1.69
22:00
2.73
206.41
<0.1
22:15
2.25
22:30
2.36
22:45
1.87
23:00
1.76
208.12
<0.1
23:15
1.78
23:30
2.11
23:45
2.45
I l l 1 -1 -- -1 1 1 1 1 1
1 1 1 l 1 1 1 I

TABLE 1 (continued)
Flow Characterization Study
November 17, 2005
Time
Flow (mgd)
Conductivity
(pmhosfcm)
Settleable Solids (mUL)
0:00
2.05
208.05
<0.1
0:15
1.92
0:30
1.66
0:45
2.31
1:00
1.98
207.91
<0.1
1:15
1.93
1:30
2.49
1:45
2.40
2:00
2.09
206.67
<0.1
2:15
2.27
2:30
1.89
2:45
2.11
3:00
10.59
207.24
<0.1
3:15
9.55
3:30
7.42
3:45
3.81
4:00
4.42
208.98
<0.1
4:15
5.97
4:30
7.77
4:45
7.65
5:00
7.96
208.21
<0.1
5:15
12.98
5:30
11.92
5:45
7.67
6:00
4.29
214.42
<0.1
6:15
13.67
6:30
8.41
6:45
7.97
7:00
14.88
208.37
<0.1
7:15
8.37
7:30
8.73
7:45
14.25
8:00
8.79
209.44
<0.1
8:15
6.37
8:30
6.60
8:45
5.06
9:00
3.41
208.64
<0.1
9:15
1.97
9:30
1.61
9:45
1.52
10:00
1.75
213.61
<0.1
10:15
2.34
10:30
2.90
10:45
2.39
11:00
2.28
209.72
<0.1
11:15
1.74
11:30
1.35
11:45
1.76
Time
Flow (mgd)
Conductivity
hos/cm
Settleable Solids (mUL)
12:00
1.84
209.48
<0.1
12:15
2.67
12:30
3.02
12:45
2.25
13:00
1.48
211.07
<0.1
13:15
1.05
13:30
1.47
13:45
2.01
14:00
2.79
211.87
<0.1
14:15
2.94
14:30
1.96
14:45
1.40
15:00
1.26
211.24
<0.1
15:15
1.87
15:30
2.06
15:45
2.29
16:00
2.61
211.01
<0.1
16:15
2.22
16:30
1.70
16:45
1.19
17:00
1.43
210.43
<0.1
17:15
2.03
17:30
2.73
17:45
2.64
18:00
2.20
211.04
<0.1
18:15
1.71
18:30
1.28
18:45
1.43
19:00
1.23
210.82
<0.1
19:15
1.84
19:30
2.30
19:45
2.61
20:00
1.97
210.58
<0.1
20:15
1.43
20:30
2.03
20:45
1.91
21:00
2.52
212.17
<0.1
21:15
2.44
21:30
2.64
21:45
1.88
22:00
1.87
210.42
<0.1
22:15
2.16
22:30
2.15
22:45
2.16
23:00
2.08
210.35
<0.1
23:15
2.33
23:30
2.08
23:45
2.12
7

TABLE 1 (continued)
Flow Characterization Study
November 18, 2005
Time
Flow (mgd)
Conductivity
(umhos/cm)
Settleable Solids (ml/L.)
0:00
1.82
210.35
<0.1
0:15
2.48
0:30
1.99
0:45
2.29
1:00
2.21
209.97
<0.1
1:15
2.49
1:30
2.01
1:45
1.84
2:00
2.45
209.46
<0.1
2:15
2.08
2:30
2.07
2:45
1.94
3:00
1.97
209.58
<0.1
3:15
2.09
3:30
2.27
3:45
2.44
4:00
2.52
210.57
<0.1
4:15
1.91
4:30
1.92
4:45
2.65
5:00
2.23
209.93
<0.1
5:15
2.11
5:30
2.13
5:45
2.09
6:00
1 2.07
212.35
<0.1
6:15
2.08
6:30
2.31
6:45
2.71
7:00
2.09
210.06
<0.1
7:15
2.62
7:30
2.59
7:45
2.19
8:00
2.05
210.08
<0.1
8:15
2.17
8:30
2.51
8:45
2.49
9:00
8.27
209.79
<0.1
9:15
12.26
9:30
7.18
9:45
4.74
10:00
3.10
209.79
<0.1
10:15
2.81
10:30
2.36
10:45
2.03
11:00
2.44
209.52
<0.1
11:15
2.31
11:30
2.23
11:45
2.19
Time
Flow (mgd)
Conductivity
(umhos/cm)
Settleable Solids (ml/L)
12:00
2.79
210.47
<0.1
12:15
2.19
12:30
2.03
12:45
2.46
13:00
2.32
209.28
<0.1
13:15
2.17
13:30
2.75
13:45
2.76
14:00
2.06
209.40
<0.1
14:15
1.91
14:30
1.92
14:46
2.30
15:00
2.12
208.59
<0.1
15:15
2.45
15:30
2.76
15:45
2.78
16:00
1.70
207.62
<0.1
18:15
1.60
16:30
2.14
16:45
2.90
17:00
2.88
207.68
<0.1
17:15
2.55
17:30
1.58
17:45
1.22
18:00
1.27
207.21
<0.1
18:15
2.39
18:30
2.24
18:46
2.26
19:00
2.11
206.77
<0.1
19:15
1.92
19:30
2.77
19:45
2.85
20:00
2.22
206.52
<0.1
20:15
2.10
20:30
2.32
20:45
2.15
21:00
2.21
205.66
<0.1
21:15
1.95
21:30
2.06
21:45
2.27
22:00
2.09
206.07
<0.1
22:15
2.04
22:30
2.17
22:45
2.43
23:00
2.36
206.46
<0.1
23:15
2.02
23:30
1.93
23:45
2.11
8
I I I I I I I I I I I I

TABLE 1 (continued)
Flow Characterization Study
November 19, 2005
Time
Flow (mgd)
Conductivity
(umhos/cm)
Settleable Solids (mUL)
0:00
1.89
205.34
<0.1
0:15
2.71
0:30
2.52
0:45
1.93
1:00
1.67
82.03
<0.1
1:15
2.02
1:30
2.21
1:45
2.38
2:00
2.18
204.11
<0.1
2:15
2.13
2:30
2.09
2:45
2.41
3:00
2.35
204.11
<0.1
3:15
2.63
3:30
2.02
3:45
1.85
4:00
2.23
204.28
<0.1
4:15
2.61
4:30
2.73
4:45
2.43
5:00
2.13
204.81
<0.1
5:15
1.69
5:30
7.91
5:45
14.23
6:00
8.24
156.73
<0.1
6:15
6.68
6:30
10.95
6:45
13.71
7:00
13.75
205.63
<0.1
7:15
6.67
7:30
8.42
7:45
13.84
8:00
14.13
206.08
<0.1
8:15
8.32
8:30
4.41
8:45
2.82
9:00
2.53
206.44
<0.1
9:15
2.57
9:30
2.89
9:45
2.34
10:00
2.58
208.96
<0.1
10:15
2.03
10:30
2.17
10:45
1.83
11:00
1.71
206.61
<0.1
11:15
2.55
11 *30
2.74
1 Z
3.26
Time
Flow (mgd)
Conductivity
hos/cm
Settleable Solids (ml/L)
12:00
2.67
206.58
<0.1
12:15
2.66
12:30
1.84
12:45
1.48
13:00
2.78
207.10
<0.1
13:15
2.51
13:30
2.47
13:45
2.19
14:00
2.38
207.02
<0.1
14:15
2.07
14:30
1.72
14:45
2.38
15:00
2.46
205.90
<0.1
15:15
2.51
15:30
10.95
15:45
9.61
16:00
6.37
206.50
<0.1
16:15
11.85
16:30
8.88
16:45
6.14
17:00
10.76
206.81
<0.1
17:15
14.04
17:30
8.22
17:45
4.14
18:00
2.45
207.33
<0.1
18:15
2.01
18:30
1.84
18:45
2.03
19:00
3.55
208.01
<0.1
19:15
2.74
19:30
2.36
19:45
1.80
20:00
1.59
9.10
<0.1
20:15
1.71
20:30
2.03
20:45
3.01
21:00
2.49
209.05
<0.1
21:15
2.44
21:30
2.29
21:45
1.97
22:00
2.12
207.86
<0.1
22:15
2.00
22:30
3.17
22:45
2.56
23:00
2.71
210.58
<0.1
23:15
2.23
23:30
1.62
23:45
1.56
9

TABLE 1 (continued)
Flow Characterization Study
November 20, 2005
Time
Flow (mgd)
Conductivity
mhos/cm
Settleable Solids (ml/L)
0:00
1.74
208.08
<0.1
0:15
2.31
0:30
2.12
0:45
2.52
1:00
12.51
209.44
<0.1
1:15
13.18
1:30
8.31
1:45
6.39
2:00
2.96
208.83
<0.1
2:15
2.42
2:30
2.54
2:45
2.57
3:00
2.25
209.72
<0.1
3:15
1.83
3:30
1.67
3:45
1.73
4:00
2.09
209.59
<0.1
4:15
2.31
4:30
2.58
4:45
2.74
5:00
4.01
211.06
<0.1
5:15
8.75
5:30
8.64
5:45
8.28
6:00
7.47
213.33
<0.1
6:15
5.66
6:30
12.81
6:45
8.59
7:00
6.25
211.64
<0.1
7:15
4.13
7:30
3.01
7:45
2.35
8:00
2.21
10.54
<0.1
8:15
2.17
8:30
2.47
8:45
2.74
9:00
3.17
213.24
<0.1
9:15
1.97
9:30
2.01
9:45
2.31
10:00
2.57
213.34
<0.1
10:15
2.82
10:30
2.38
10:45
2.19
11:00
1.81
215.73
<0.1
11:15
2.03
11,30
2.15
11:45
2.66
Time
Flow (mgd)
Conductivity
mhos/cm
Settleable Solids (ml/L)
12:00
2.91
214.75
<0.1
12:15
2.47
12:30
2.62
12:45
2.06
13:00
1.57
218.88
<0.1
13:15
2.07
13:30
1.90
13:45
2.69
14:00
2.11
213.96
<0.1
14:15
1.96
14:30
1.53
14:45
1.38
15:00
1.62
218.06
<0.1
15:15
1.70
15:30
2.48
15:45
2.90
16:00
2.71
213.59
<0.1
16:15
1.99
16:30
1.53
16:45
1.86
17:00
2.39
219.95
<0.1
17:15
1.87
17:30
1.82
17:45
1.89
18:00
1.81
217.74
<0.1
18:15
1.83
18:30
1.48
18:45
1.63
19:00
1.81
213.41
<0.1
19:15
2.38
19:30
3.05
19:45
2.21
20:00
1.56
213.28
<0.1
20:15
2.17
20:30
1.99
20:45
2.17
21:00
2.29
215.44
<0.1
21:15
2.21
21:30
2.13
21:45
2.07
22:00
2.32
213.56
<0.1
22:15
2.46
22:30
2.21
22:45
2.33
23:00
2.61
209.90
<0.1
23:15
2.76
23:30
2.89
23:45
3.58
10

TABLE 1 (continued)
Flow Characterization Study
November 21, 2005
Time
Flow (mgd)
Conductivity
mhos/cm
Settleable Solids (ml/L)
0:00
2.56
195.59
<0.1
0:15
1.85
0:30
2.01
0:45
2.52
1:00
3.33
196.02
<0.1
1:15
2.78
1:30
2.27
1:45
2.08
2:00
1.81
195.68
<0.1
2:15
2.14
2:30
2.73
2:45
3.13
3:00
2.79
213.46
<0.1
3:15
1.92
3:30
1.73
3:45
1.55
4:00
2.16
209.91
<0.1
4:15
2.31
4:30
3.15
4:45
2.17
5:00
2.07
206.25
<0.1
5:15
1.97
5:30
2.29
5:45
2.44
6:00
2.93
210.78
<0.1
6:15
2.75
6:30
5.57
6:45
2.39
7:00
2.25
211.31
<0.1
7:15
2.05
7:30
1.92
7:45
2.32
8:00
2.68
211.98
<0.1
r
1
i
7-day Average
3.16
203.38
<0.1
7-day Max.
14.88
219.95
<0.1
7-day Min.
1.05
4.32
<0.1
11

Figure 1. Flow Characterization Study
30
220
28
200
26
— -
180
24
22
-
-_
160
20
140
a
18
0
16
120 <:
E
�
0 14
-
100
LL
=r
12
— --
- =
0
80 0
10
_
- - _
3
v
8
- -
60
6
- - --
40
4-
20
2
0
0
11 /14/0 11 /14/0 11 /15/0 11 /15/0 11 /16/0 11 /16/0 11 /17/0 11 /17/0 11 /18/0 11 /18/0 11 /19/0 11 /19/0 11 /20/0 11 /20/0 11 /21 /0
5 6:00 5 18:00 5 6:00 5 18:00 5 6:00 5 18:00 5 6:00 5 18:00 5 6:00 5 18:00 5 6:00 5 18:00 5 6:00 5 18:00 5 6:00
Time
- Flow
Conductivity

APPENDIX A

04
1LI 141 LUUU LJ: YO Gq JUPIIYDun WHIMM rLl rmU ULILY
Effluent Toxicity Report Form- Chronic Pass/Fail and Acute LC50 1
Facility CM iD NPDES#NC6W& M37ti Pip# Opp
e County
Laboratory Performing T st Mar rr`r r � �.
�-�[ I �—
x — I comments
il'13-0.5"
Signature of Operator in poosi to Charge
x�
Signatureioraoq,' Ju Nisor
r
nmental SCiences 6ig
Div. o r Qual'
N.C. DEN
16 ervice
North Carolina Cerlodanhnla Chronic Pass/Fail Repro-duct!o oxi i Test igh, North Carolina 27 t
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Chronic Test Reaulta
#Young Produced Calculate­
1
Tabuiet C
Reduction
Adult (L )Ive (D )ead PIVIoltallty I Av ,I
r
Effluent%I—Control
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Treatment 2 Treatr
.. Control CV
# Young Produced PASS
M I Adult (L Jive (D )ead
1stsample lstsam le 2ndsample
Control
pH
Treatment 2
�. t a l e 9
a n a n a e
r d r d r d
t t t
tat sample lstsampie 2ndsampie
Control F Ell H
D.O,
.. Treatment 2
LC501Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Sample 1 I I / J
Sample Tvoe/Duration
1
2
%l
%
%
%
%
Y
%
o
%
D%
�%
LC50=_%2 %
79�5%� �C�onfidginncce�.Liiimits
... organlsm Tested
DWQ fort AT-1 (3/97) rev, I I/9S
Method of Determination
Moving Average © Probit
Spearman Kerber ❑ Other__
01
Duration
Hardness(mg/1)
Spec,Cond.(pmhcS)
Chlorine(mg/1)
Sample temp. at receipt
Concentration
Mortality I Note; Please
Complete This
Section Also
starttend start/end
Conho
y High 7 7
Conc.
34
pH D.D.
Check One
sl c,lprrtt GI at-
( I C(
2 i /
Note: Original submitted with monthly Daily Monitoring Report
(DMR) for November.

v
CV
m
m
w
cD
CT
IL
a
w
a
z
0
W
z
0
W
Cr)
Cn
OD
N
1
!D
N
(10
1
(n
Cn
W
m
N
U)
!D
W
CV
C�1
e-1
Client:
NPDESt#: C �' 710
Analyst:
Dilution Water:2,u�Sv it
Randomization?. 10 &J6
Incubator#:
Merltech. Inc.
Data Sheet for Acute Toxicity Test
48 Hour Acute Cerlodaphnia
No. of Organisms per Chamber. ...J
Test VF.s8el Size: 10 ML
Test Sollttian Volume: .2bin
L .—
No. of Replicates per Treafrnent: `t
v
Temp. or Stock: G.
Test Organism Source (Tray #):
Start: DateTime:
End: Date1 Time: We,
Test Organism _"j4 j Age: "2 S gpj).A
Born: Date: I- v-D.- T iime: SP°-7--,ff)jS-? j
Test Termtnated by: /'j-
Reviewed by:
tration
FMooM
106�
M
I =
MM
MP#M
o
r,�
ePill.
eeee�e,����e����
oo:4!
omom000
MMMMEEM-MM
�ee�e��eeen����■����
eoeeeeeeo�����■���
LMFam,
M
IMMOMEMaW
MeMer�MM6
9,01MM''
MM
Total Hardness as mgfl of GaCO 3
Date and Time Fed: ,� "�. /Dorm
Initials:

1 u 1 FJ [CJCJ� tJ: L+0 71 7-o r 0-[o7J
ci*I oun izuw wt t i r-m rt, i - ... -: s•►
EM Johnson
Proportion
MM
Conc.
Number Number
Observed
proportion
Responding
adjusted for
Exposed Resp.
Responding
Controls
-�
10.0000
20.0000
20 0
20
0.0000
0.0000
�0.0000
1
20 1
0.0500
0.0500
fan
65.0000
20 6
0.0500
0.3000
0.0500 '
0.3000
90.0000
20 10
0.5000
0.5000
Chi - Square
for Heterogeneity
(calculated)
_ 2.196
Chi - Square
for Heterogeneity
(tabular value at 0.05
level,)
7.815
EM Johnson
Estimated LC/EC Values and Confidence
Limits
95% Confidence
Limits
Point
Conc.
Conc.
Lower
Upper
LC/EC 1.00
16.706
3.211
27.569
LC/EC 50.00
96.600
73.311
195.304
fm
am%
Oft
0M
IM
MR
M
am

1'1I 14/ 1bb5 :e.J: 4b yly-d Ill-zUtij tM JUMMUN WH I tK rL. I rHuc riot L4
AMerxtech, Ihc.
Bioassay Sample Chain of Curod, 642 Ta m eo Rd
Reidsville. NC 27320
` [phone]
Client Information
Client: Ci.. �t�o PO # Poi � 4 ,2q'T
Contact: � NPDES 1#: N
Address: l o ( s) A.3ib
ity:
phone:1.70
��.�,
County: w>�1C ,k- State: Pipe Number:7
Type of Facility Generating Effluent: lYlun. c_4
Sample Information
Sampling Site:_, N p DES oo jEj,+
Sarnplc Type: Grab Composite # of Contain s f
Sampling Tame: START Date: j (- l5 -OS Time: Qq O W- PM
END Date: N/A Time: N/Iq AM PM
Sample Chilled (Covered with ice): Yes No
Collector's Name (Prig): are
Collector's Signature: r0p_M0 �
Tox,ieity Test Information
Test Required; Chronic: Vest Organism: Ceri.odapluda Dubia:— s/ -
Acute: — y/ Pimephales promelas:JWC _
Mysidopsis baiva:
J oxiciry Test Concentration(s):
List Any Special Requirements:
Shipping Information
Relinquished AQ Date:
Time: !D Am PM
Received By: Date: T) � _o' Tirne:Jej e2 AM PM
Relinquished By: Date: ll=,I,S :p ime,,-f, M PMReceived By: Date: Time: . AM P.M
Sample Temperature (C : Method of Shipment: WTC(p/u) UPS Fed -Ex
Circle One Other:
Sample Receiving (Laboratory Use Only)
Received From: fie=
Received By: . Date: L C S Time:'AM
Sample Temperatuz�e {C ): / Samp.le ondition: C�
,am(
0M
"a
004
w
am
.,
oft
a"
No
.e
0"
0"
MR

1Lf lYf LVVJ LJ. YY J1J Vf V LV JJ LI•I VVI II\JVII W.II LI\ I LI 1 i14L VV. ��
Effluent Aquatic Toxicity Report Form/Phase II Chronic Ceriodaphnia
Faclilly E►'n .�o�'4n9Grt NPDES#:NCe2jL2. to Pipe#00 County o.hq- _
Laboratory Performing Testjii Comment ar� . S LqS �lo,ry�;ya n
x x i[a--r .`Pie �, Cl lar)nt Itt-A-o•1'Or/'Cr` Ar
Signature of O.R.C. Signatureof Lab Supervlsor I �'1Dn ' rOr^ u"''"'
Sample Informatio
Collection Start Date
Grab
Composite
(Duration)
Hardness(mgA
Spec.Cond.(umhos/c
Chladne(mg/I
Sample temp. at receip
n
Sample 1 Sam le 2 Control
I-r -ps O!;
aµ s
c'{0(p alU /'7 E
c o.
0.
1.4 'c o. s °c
oian u ne I End Data Stan Time End
11 /1s/gs rr/. 5 9 :
Start RenewilkOmw2l Stan Renew Re
1001 1 o0'j Control Ccrdrol OM
pH Initial 1.16
pH Final °(, & I
Q.O.lnitia 6.Ia
D.O. Final 7.55
lmp, initial aq,8
-mp. Final 1.1q. G
Organism#
1 5 6 7 8 9 10 11 12 mew
Control #Young all I I B AO ]o is 19 19 oat
Adult
(L)ive (D)ead L L L. L L L L-
1 2 3 4 8 6 78 911 10 11 12 Won
Effluent% #Young a0 21 I 1'1 a3 a5 eZ0 to I 1q•�
-Zz Adult xa°a
(L)Ive (D)ead L L. L- L L L L L L- 3.0
MMMn
Efflu�enntt% #Young i 1 a3 b IQ ao ao I o 1 as
Adult %Rm Method:
h (ve Dead L L L L L L 1, 'L I L. H2O.Normal
Effluent% #Young 18 lb 11 IB Al as AO
7Jr Adult
(L)Ive (D)ead L L L L L L L L
Effluent%
$0
1.3101• V 141441.14.
:hro is Test Remits
at Control Mortality °
°/M Con lral 3rd Broo4 lot
%
Lj"r�l Non-ParametdoAnelvsis(ifaopllcableY
Method:
M°e1 Effluent % Rank Sum Cdtica(Sum
#Young K. 11 1 16 1 It. 1 17 1 1'7 110 1 11 111.111 14 H1440,-004
(L)(Ve (Dteatl L L [LIL 'L L_ L L t_
1 2 3 4 5 6 7 8
Effivant°lo #Young IS119 F1411611SILIA,1 i 1'd
It?0 Adult
(L)ive (D)ead L L L L I L_ I L L I L
MAIL ATT: Environmental Sciences Branch
Div. of Water Quality
TO: N.C. DENR
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Should use highest teat concentration or
highest concentration with D.O. 15.0 mgA
T I RMMu°MMn M Con"I RUMduMlon 14sen
DWQ form AT-3 (8/91) Rev. 11/95
oian u ne I End Data Stan Time End
11 /1s/gs rr/. 5 9 :
Start RenewilkOmw2l Stan Renew Re
1001 1 o0'j Control Ccrdrol OM
pH Initial 1.16
pH Final °(, & I
Q.O.lnitia 6.Ia
D.O. Final 7.55
lmp, initial aq,8
-mp. Final 1.1q. G
Organism#
1 5 6 7 8 9 10 11 12 mew
Control #Young all I I B AO ]o is 19 19 oat
Adult
(L)ive (D)ead L L L. L L L L-
1 2 3 4 8 6 78 911 10 11 12 Won
Effluent% #Young a0 21 I 1'1 a3 a5 eZ0 to I 1q•�
-Zz Adult xa°a
(L)Ive (D)ead L L. L- L L L L L L- 3.0
MMMn
Efflu�enntt% #Young i 1 a3 b IQ ao ao I o 1 as
Adult %Rm Method:
h (ve Dead L L L L L L 1, 'L I L. H2O.Normal
Effluent% #Young 18 lb 11 IB Al as AO
7Jr Adult
(L)Ive (D)ead L L L L L L L L
Effluent%
$0
1.3101• V 141441.14.
:hro is Test Remits
at Control Mortality °
°/M Con lral 3rd Broo4 lot
%
Lj"r�l Non-ParametdoAnelvsis(ifaopllcableY
Method:
M°e1 Effluent % Rank Sum Cdtica(Sum
#Young K. 11 1 16 1 It. 1 17 1 1'7 110 1 11 111.111 14 H1440,-004
(L)(Ve (Dteatl L L [LIL 'L L_ L L t_
1 2 3 4 5 6 7 8
Effivant°lo #Young IS119 F1411611SILIA,1 i 1'd
It?0 Adult
(L)ive (D)ead L L L L I L_ I L L I L
MAIL ATT: Environmental Sciences Branch
Div. of Water Quality
TO: N.C. DENR
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Should use highest teat concentration or
highest concentration with D.O. 15.0 mgA
T I RMMu°MMn M Con"I RUMduMlon 14sen
DWQ form AT-3 (8/91) Rev. 11/95
1 2 3 4 5 6 7 8
Effivant°lo #Young IS119 F1411611SILIA,1 i 1'd
It?0 Adult
(L)ive (D)ead L L L L I L_ I L L I L
MAIL ATT: Environmental Sciences Branch
Div. of Water Quality
TO: N.C. DENR
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Should use highest teat concentration or
highest concentration with D.O. 15.0 mgA
T I RMMu°MMn M Con"I RUMduMlon 14sen
DWQ form AT-3 (8/91) Rev. 11/95

IV 14/ 'ebbP 'L.i: 4b yl y-b t b-ZU1j j tM JUHNSUN WA I tK I L I I-'Alzt b t / 14
a•
-•�.�' 1=SVVIROhtMBl�lT L L4t30RATORt�S
e�Rs/may wrsd COr►lrot.4. /ne.
Multi-diiutionai Ceriodaphnia dubia Chronic Definitive Test Data Sheet
T@6f Effluent Information;
Client: 'INS 'S p N 3DES #: ' G 008 Z? -?
ea
Dilution Water: take_Reidsa! Batch # H d r---.n, __ ..
ar nese
qq?, I I q4o
Collection Start
Sample Type / Our,.
Conductivity (Umhos
Residual C Ij InrL
Temperature
100°/
Incubator M. I
Temp. of Stock: °C
Randomization: & No
Test Start, Renewal, and End Dates 1 Times:
Start Date; Time:
1st Renewal Date: IV - 1-7- iD Time:
2nd Renewal bate. Time:
End Date: Time: ���.�
Chemical and Physical Determination*-
C
Stirred Aerated
Neonates:
A ,—
sfer: DateMme Z/-/ rs-/ 00m
dateMme //,i . of/ r'; r � COPA-?
t Stem of Test: A� hours
Test Transfer:
Feeding:
Start:
Start:
*4
Day 1: .(k-
Day 2: /r�
Day 2: „.,'M L
Day 3: /*-
Day 5:
Day 4:
Day S; /"k
inated:
Day 6: �L
Test
Start
1 at Renewal
2nd Renewal_..,.
Start
End
tart
End
Start End
Control
PH
7- 6
1.94
'T.
High Conc.
7. 15
rj, (��
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D.O.
�`��
'7•'7a I
11•
0
6q 5
High Conc.
. �.
�..3r' 6
rT.
t
I.
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temp,
Z , $�
t1 4
a �
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g4
.�
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c'�q.S
ia4. 3
�/!����~11R�i Soli 3 �v►Sp 1e1 Sam�d �X I 3V1+R 'TMS� Cin+O1 Irlt
9s r�•y .�� Spc. �hlorinc �� T x t we 9� .�
S ��M �L 4 �Ec. AV%O
dMR
P"
P"
me

`%
Meritech
Cerlodaphnia Chronic Reproduction
LIC
Client: EK4 '�o�vLso�t
KFPDES-4
'--—?�?."��
Data Sheet
Start Date: r1 s 03 Time:
•�__
_ .
End Date:.
.
Time: .....
9�6Eftlu�nt paY#
9
i.�
Reviewed by: -
Replicates
3
�
O
3
�
5
6
_ 7 8
_�
9
100
, 3rd road
Control!
0
a
O
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d
4 t
C7
r
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a
7
7
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YAY'y
$`
C.V-. Mead
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Total
2.
t
j
2
Reorodaction
w
a
3
o
b
o
o
ot
2-0
Z
--�r!
7
y�y
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T
Total
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2
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•
o
! 7
23
!o RED.
3. E,1
w
3
0
6
Q
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Total
17
Z3
i�u
--
°% RED., D
-
3
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Total
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r�
2z-
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m
2 r
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% RED.
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7
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1Z/14/ZU170 Z3: 4b 71'7-tl /tl-Ztl7d LM JUHNbUN WHILK F'LI r/aUL UW/ e4
rn
Meritech, Inc. '2 / —
Bioarsay Sample Chain of Custody
642 Tamco Rd
Reidsville, NC 27320
(336) 3424748
Client information
Client: C' iva{ Qol rn�ohn-son wTP PO �7ap64aq^1
Contact; ` `Tohwcr'n NPDES #: N
Address: I City:_Ph
I�� 'N - — �.tZc�o 8� 3'7io
one:qlG- o-�b70
F .�Pipe Number: ppa.
Countty:y: State: NC Tin: 27 (a I a
Type of Facility Generating Effluent: lYlurlsGipa.l W0.}ew pbCt� _
Sample Information
Sampling Sits: 00 F
Sample Type: Grab _%-/ Composite # of Containers 3.
Sampling Time: START Date: I -11 ri - O Time: pq p p PM
END Date: ^iI -IS-OS Time: Q°YOO PM
Sample Chilled (Covered w h Ice): Yes ✓ No
Collector's Name (Prim: rt _
Col.lector's Signature: _am
Toxicity Test Information
Test Required: Chronic: 9 Test Organism: Cetiodaphnia Dubia:
Acute: Pimephales promelas: ..
IWC: G oho
Mysidopsis bahia:
toxicity Test Concentration(s): 00 r�
List Any Special Requirements:
Shipping Information r*� —`-
Relinquished By Ani Date: J -1$. Time: 1 QW (! a PM
Received By; Date: // -!�r_o Time: 2l AM PM
Relinquished 13y: Date: / � Time: f � I S"AM PM
Received By: Date: Time: , AM pM
Sample Temperature (C ): Method of Shipment: WTC(p/u) UPS Fed -Ex
Circle One Other:
—
Sample Receiving (Laboratory Use Only)
Received From:
Received By: J Date: O `Z- Time:X� AM
Sample Temperature (CM L- / .a Sample ondidon:_ c,7 _
r

1L/14/GOU0 LL:40 Cpl JUMNY UIN WHI LM rl.l 1. .,n 1C414
M
go
Meritech, Inc.
Bioassay Sample Chain of Custody
642 TemeO Rd
Reidsville, NC 27320
[phone]
Client Information
Client: C. V 1° PO» OO/v d 5 Contact; � NPDLS #:
Address: r' (\1_c ODoS237tm
City: _LQ3 �tv?si,_ Phone:'�_�g'd
County:- State: Pipe Number:
NC Zip:,_22�
Type of Facility Generating Effluent: (Y1 u n l G ctlz�l
Sample Information
.. Sampling Site: N P=S O ur�^tt
Sample Type: Grab _ // Composite # of Containers
Sampling Time: START Date; _II - U-p 5 Time:�j$� (g toPM
END Date: 1l -if -oS Time: W145 (0a PM
Sample Chilled (Covered with Ice): Yes ✓ No
Collector's Name (.Print): ge aM
^ Collector's Signature; _ la6ur�
Toxicity Test Information
Test Required: Chronic: i' 11. Test Organism: Ceriodaphnia Dubia: ✓
Acute:
Pimephales promelas:____
1IWC: p
Mysidopsis bahia:
toxicity Test Concentration(s):
qp 7S u a.5
List Any Special Requirements:
Shipping Information
Relinquished By:
Received By: s?..I
Date: /-/ S" Time: 4' ff M
�yM
Relinquished By: _gyp IY �
Date:1 i- 6 e Time: Ii;
Date: t t, 19, c l Time:
Received By:
Sample Temperature (C ):
Date: Time:. AM PM
Method of Shipment: WTC(p/u) UPS Fed-&
Circle One Other:
Sample Receiving (Laboratory Use Only)
Received From: -t}�
Received .By: _ 7 < (n o o Date: 1 Time: oiAP
Sample Temperature I^' (
(C�): /O,S
Sample Condition: tC
0%

1LI 1441 LUU:J GJ. ` 0 71 7-O f U-LO7J GI'1 Owl-111.0 JIY Whi 1 Ll\ I Ir I I nu►_ fir c't
• c
Title: EM Johnson
File: EMJ Transform. NO TRANSFORMATION
_ Kolmogorov Test for Normality
r-- r r- r r r- r-- r-- r r r w i- •. r-- w r r r- r r r
1 r r - -r- r - r-r r r r r
w r — — r r
D 0.9168 = �
0.169
D'*(p-value = 0.0405)
=
Critical D* = 1,035 (alpha 0.01 , N = 60)
= 0.895 (alpha = 0.05 , N = 60)
.. r ..•..... r r r � r � .� � r r � w r r � r .. � �. r `. � ti r � r r .. r � r .r � � r .. � .� r r r r .. r � � � w r � � r �. � _. .. .. .. ._ .. ... ._ .. -. r � /'�
Data PASS normality test (alpha = 0.01). Continue analysis.
am
ON
AA

1L/ 14/ 'LYJl7b L�: gib �l b—t� I17—LCfb.7 tM JUMMDUIY WH I r-M r L.1 r iAkAt 1 L/ ic}
Title: EM Johnson
File: BMJ
Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------
"" Calculated B1 statistic = Z2,�7g0
Data PASS B], homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df � 5)
- 11.0705 (alpha = 0.05, df
t
a�
am
FOR

1'L/ 14/ :630ti
'L.1: 4b 1�1'y-U I U-'Lti J.j
tM JUMMDUY
WH I tK h'L I
t-,A L I J/ 'L4
Title:
EM Johnson
File:
EMJ
Transform!
NO TRANSFORMATION
Summary Statistics
on Data
TABLrE I of 2
GRP IDENTIFICATION
N MIN
MAX
MEAT
1 control
2
10 18.0000
24.0000
22.5
3
10 16.0000
25.0000
19.4000
45
4
10 16.0000
25.0000
19.6000
75
5
10 17.0000
22.0000
18.7000
6 90
10 14.0000
17.0000
16.2000
WO
-----
100
_ .. r r __ r -_ �.r_ _ r
10 13.0000 19,0000
--------
15.5000
Title:
E'M Johnson
File:
EMJ
Transform:
NO TRANSFORMTION
w
- - - - - - - -
Summary Statistics
- - - - - - - - - - - - - - r r _ - .. - .. -
on Data
- - - - - - - _ r _ _ _
TABLE 2
- - _ - - - - r
of 2
r
GRP
--- -
IDENTIFICATION VARIANCE SD
-,--_ ---r------ --- _
SEM
_ - - - - - - r ` - - - _ r - - -
C.V. �
2
control
22.5
3.1211 1.7638
r r.
0.5578 8.8192
3
45
8.9333 2.9889
0.9452
15.4065
4
75
7.3778 2.7162
0.8589
13.8582
5
90
3.1222 1.7670
0.5588
9.4491
5
100
0.8444 0.918.9
0.2906
5.6724
- - - - _ _
4.9444 2.2236
0.7032
14.3459
pffM
MM
ow
00
oft
Am

114/ 114/ LCJCJV L3: LFO 71 7-a f V-LG7J
UVI JUMNIZU114 WH 1 MM rL. 1
rHuG 141 LLF
- Title: EM johnsor,
File;
EMJ
Transorm:
f
NO TRANSFORMATION
_
ANOVA Table
SOURCE
DF
---------._
-------------- - - - -
- - - - - - - - -
SS
- - - -
MS F-
Between
5
- - r .. _
181.7333
36.3467 7.6999
Within (Error)
'S4
255.0000
---Tota1__--__
59
436.7333
(iP-value = O. o00o)
P"
w�l
FOw
am
Critical F = 3.3769 (alpha 0.01, df = 5154)
= 2.3861 (alpha 0.05, df = 5154)
Since F > Critical F REJECT Ho: All equal (alpha = 0,05)

IV 14/:MUb
:e.j: 4b yl y-U f U_:ed1jj
tM JUMMDUN WH I tK VL 1
1
Title:
EIw Johnson
File:
EMJ
Transform: Y(` .
y ti �•t ry /��T
Dunnett ' s Test -
TABLE J. OF 20 : Cor�tx'ol
cTre atment
GROUP
IDENTIFICATION
MEAN CALCULATED -III
M�
SIG
'
_
ORIGINAL UNITS
T STAT 0.05
3.
2
control
20.0000 20.0000
�.►
3
22.5
19.4000 19.4000
0.6174
4
45
75
19.6�000 19,6000
0.4116
5
90
18.'7000 18.7000
1.3377
6
100
16.200Q 16.2000
15.5000
3.9102
M'
--------
_ ... _ _ _ _ 1 r _ _ _ _ _ _ _ _ _ _
15.5000
_
a.5305
Dunnett
_ _ _ _ - - _
alpha _-0-...
Critical value = 2,3100 (1 Tailed, d
. , 05f
[used)_,= _-5140)__
(Actual df = 5, 54•)
r
Title:
EM Johnson
File:
EMJ
Transform: NO
TRANSFORMATION
D=nett's Test
----------------`----- --
FABLE 2 OF 2 Ho: Control<Treatment
GROUP
XDENTI FICAiTION
------------
NUM OF MIN SIG-DIFF OF
REPS (IN ORIG UNITS)
DIFFERENCE
.r,7,�D
- - - - - -
- - - - - - - - -- - - - - _ - -. - r
. CONTROL
�. r _
F .., � e, ^r
�O� �0WR0L
control.
... _ r _
10
2
3
22.5
45
1.0 2.2449 11,2
0.6000
4
75
10 2.2449 11.2
'10
0.4000
0.4000
5
90
2.2449 11.2
10
6
2.2449 11.2
10
3.80or
--------------
2.2449 11.2
4.5000
-Y-_^--_-100
4"
ow
Inq

M1
,am
on
P"
No
oft
M"
Fma
1 LI 14/ ,4000 ZJ: 40 71 -CI ! U-Gi3J3 tin JUP'MUN WH 1 tK rL 1 f Hlit 10! !4
- M
IN
mE
kRI-TECH, it
ENVIRONMENTAL LABORATORIES
A Division Of Water 'rechno/o
gy and Controls, inc.
Memo
Date: 11/22/05
To: EM Johnson
Subject: Interference of solids in EM Johnson Toxicity sample one.
The EM Johnson Municipal water Plant for the city of Raleigh
(NC0082376) ran their required November multi-dilutional chronic toxicity
Test on 11 / 15/05. At test initiation, total residual chlorine for sample tox I
(11114-15/05) was performed and recorded with a value of 2.54mg/1. All of
the higher dilution organisms should have died at that concentration of
chlorine. However, on 11 / 17/05 (first renewal date) it was noted that none
of the test organisms had died. The sample was then re -ran for chlorine on
11/17/05 with similar results. Amer filtering sample tox 1 through, a #3
whatznan filter to remove all the suspended solids, the chlorine test was run
again, This time there was no indication of any residual chlorine (<0,1
rng/1).
Based on this data it was concluded that the solids in. EM Johnson tox
1 sample caused a false reading with regard to residual chlorine. After
discussing the issue with Matt Mathews of the State of NC aquatic
Toxicology unit it was concluded that a memo should accompany the
original toxicity test in an attempt to explain the circumstances of this
situation and to clear up any confusion surrounding this matter.
Michae Ree
ioassay Dept.
Meritech, Inc,
642 Tamco Road 11 P.O. Box 27 * Reidsville, NC 27320
[phone] - [phone] Fax

ltf 1"II LVVJ LJ. 'TV Ji. J Ulu LU JJ
aceA zWical
www.pocelabs.com
November 30, 2006
Mr. Larry McMillan
City of Raleigh Public Utility
PO Box 590
Ral•ei gh, IBC 27602
RE: Lab Project Number: 92107977
Client Project ID: C.dubia Chronic
Dear Mr. McMillan:
L.1.6 V VI tl 1.JV1 Y YYt"1 I `IN 1 L.. 1
race Nnsiptal services, lnc.
9800 Klncey Avenue, Suite 100
Nuntersville, NC 28078
Phone, 704.8i6,9082
Fax., 704.875.9091
t nut_ J. r r L-+
Pace analytical Sergices, Inc.
?.225 Riverside Drive
Asheville, NC 28804
Phone: [phone]
Pax.- 828.252.4618
Enclosed are the analytical results for sample(s) received by the la oratory November 15. 2005through November
18. 2005 Results reported herein conform to the most current NELAC s andards. where applicable. unless otherwise
narrated in the body of the report.
Inorganic filet Chemistry and Metals Analyses were performed at our Pade Asheville laboratory and Organic testing
was performed at our Pace Charlotte laboratory unless otherwise footnoted.
If you have any questions concerning this report, please feel free 4 contact me.
Sincerely,
Jjennif Jones
[e-mail]
Project Manager
Enclosures
A-shMtb Cart' cation
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL NELAP F87648
I
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, exceF t in full,
without the written consent of Pace Analytical .' ervlces, Inc.
*0n
ac*.,
Char tkUert fic tin c
N-C Wastevratsr :2
NC Drinking Water 37706
SC 99006
FL NELAP E87627
.r,
oft
r�1
ow
�'
0"
Oft
am
r'4

1L/ 194/ ZU00 LJ; CFO 71,J-0 f U-L6ZIJ
ace Analytical
www.Raaelabs.eem
C-11 JUMN-nUN WH I r-M rL 1
i al,e Auldirlival J8Nice$1 1110.
9600 Kinsey Avenue, Suite 100
HUntersville, NC 28078
Phone. [phone]
Fax.[phone]
rHuG 10/ LH
Pace Analylleal Sely/ces, In-.
2225 Riverside Drive
Asheville, NC 28604
Phone.' 828.254.7176
FIX." 828.252.4618
Lab Project Number: 92107977
Client Project iD: C.dubia Chro;-;,
lab Sample No: 926369703 Project Sample Number: 92107977-001 Date Collected: 11./14/05 09:00
Client Sample T0; COMPOSITE FFF.
Matrix; Water Date Received: 11/15185 37:20
Par eters sulfi Units Report Limit ,- Analyzed B y C�5_No. _ ua7 a Lmt
Bioassay
C.dubia Full -range Chromic NC Method: EPA 600/4.91/002
C.dubia Full range Chronic NOEC=100x 11/16/05 .1GJ
Date: 11/30/05
AWhevill ,ertiiic .IDS
NC Wastewater 40
NC Drinking Water 37712
SC 99030
FL NELAP E87648
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
Without the written consent of Pace Analytical Services, Inc.
�4 ■ f�. r
Page! 1 of 4
Char _C,ertliicatlen Qra
NC Wastewater
12
NO Drinking Water
37706
SC
99006
FL NELAP
E87627

1L/ 14/ Zt3rJO L3: 40 717-0 to_z f33
_ ace Analytical
is ww,Peceia6s.cam
GIYI JUMNnUN WH I tK I-L I
race Analytical Services, Inc.
9800 07cey Avenue, Suite 100
Huntersville, NC 29078
Phone: 704.875,9092
Fax: 704.875, 5091
f-AUL 15/ L4
Pace Analytical Services, inc. ""
22.25 Riverside Drive
Asheville, NC 28804
Phone: [phone]
F?, • 828.2,5,74618 04
I-ab Project Number: 92207977
Client Project IN C.dubia Chronic
Lob Sample No: 926369711
Client Sample T0; 2N0 COMMPOPO SITE EFF.
Project Sample Number: 92107977.002 Date Collected: 11/17/05 09:45
Client
Matrix: Water Date Received: 11/18/05 21:30
Parameters
R^5u1 _Uni t� Report t_i mi t Ana Iyzed 8.y CAS � 8�Lmt
Bioassay
C.dubia Full -range Chronic NC Method: EPA 600/4.91/OOT.
C,dubir Full range Chronic - 11/19/05 JGJ MW
Date: 11/30/05
A.�vi11e Certifi�atton I,p,A REPORT OF LABORATORY NC Wastewater 4o ORATORY ANALYSIS
NC Drinking Water 37712 This report shall not be reproduced, except in full,
SC 99030 without the written consent of Pace Analytical Services. Inc.
FL NELAP E87648
rr
=n'
c r Y
ISM
S�tisrSi;�Certificatio i t s "~
NC Wastewater
12
NC Drinking Water
37706
SC
99006
FL NELAP
�.
E87627

1LI 1�F/ LCJCJ:J GJ. CFO 71 7-O f CJ-L07J Giro Jurim)ur1 wH I CR r'L. I -Hkzr- LGN Z-4
race A11a1ytl08l SerVIGeS. Inn. �GC� t��1:iCai s617HC8s, Inc.
9800 KlnceyAvenue, Suite 100
Hunlersvive, NC 28078 2225 Rrverslde Drive
_�. 8804
ac�Analytical PhoAshnvIlle,e:
25 .7176
Phone. Phone: [phone]
Fax.' 704.875 9091 Fax.* 828.z52,4616
www.pacelabs.aom
Lab Project Number: 92107977
Client; Project ID: C.dubia Chronic
PARAMETER FOOTNOTES
Method 90718 modified to use ASE.
r�
All pH, Free Chlorine, Total Chlorine and Ferrous Iron analyses conducted outside of EPA recommended immediate
hold time.
Depending on the moisture content the PRLS can be elevated for all soil samples reported on a dry weight basis.
2•Chloroethyl vinyl ether has been shown to degrade in the presence of acid.
QED Not detected at or above adjusted reporting limit
NC Not: Cal cul abl e
J Estimated concentration above the adjusted method detection limit and below the adjusted reporting limit
HDL Adjusted Method Detection Limit
r+�
rr
Mft
Date: 11/30/05
Pape: 3 of 4
��ifl9�lLlp
,�,
NC Wastewater
48 _s
NC Drinking Water
37712
SC
99030
FL NELAP
E87648
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Face Analytical Services, Inc.
ti: •,f�
- ne act,
C.�A rlQtte Ce ' ',anon
NC Wastewater
12
NC Drinking Water
37706
SC
99006

itf 1'Y/ LVVJ LJ• 'YV J1 J V 1 V LVJ.J
ace Analytical
WWW.Pacelabs. corn
1...1.1 V VI II 1-)Wl l m-1 1 L-1N I L.. 1
race Andl ytical Services, Inc.
9800 Kincev Avenge, Suite 100
Huntersville, NC 28078
Phone., 704.875.9092
Fax:704.875.909r
1 MbiL Lit L`f
Pace Analytical Setirices, Inc.11•�
Z225 Riverside Drive
Asheville, NC 28804
Phone. [phone]
Fax; 328.252.46l8
Lab Project Number: 92107977
Client Project ID: C-duhia GnrD-•-
QUALITY CONTROL DATA PARAMETER FOOTNOTES
Consistent with EPA guidelines, unrounded concentrations are displayed and have been used to calculate N Rec and RPD values.
w
LCS(D) Laboratory Control Sample (Duplicate)
MS(D) Matrix Spike (Duplicate)
DUP Sample Duplicate
ND Not detected at or above adjusted reporting limit
NC Not Calculable
1 Estfmated concentration above the adjusted method detection limit and below the adjusted reporting limit !�R
MDL Adjusted Method Detection Limit
RPD Relative Percent Difference
Date: 11/m/OS
04
0"
61L
.m
r
14
OR
0M
Page: It of 4 r
gs Ile ltiflcatian !Ds PM
NC Wastewater 40 REPORT OF LABORATORY ANALYSIS har! a Certitic�tion !Ds
NC Drinking Water 37712 This report shall not be reproduced, except In full, NC Wastewater 12
so 99030 without the written consent of Pace Analytical Services, Inc. NC Orinking Water 37706
FL NELAP E87648 1N �e<o SC 99006 am
FL NELAP E87627
so

iL1 14/ ZriMJ Z.D. NO 71 7-O f u-ZoDa Clrl JUrINMUl`I wm I CrC r"t_ I I -Mk= LLI Z4
MR I Pace Analytical Services, Inc. Pace Analytical Servlres, Inc.
9800 KlnCOYAVenue, Suite 700
1� % Nuntersvllle, NC 28t?78 2225 Riverside nr.'tlO
� n A! lal ,�ca'O Phone. 704.875,9092 Asheville, NC �8804
Fax., 704.875.9097 Phone. 828.Z54.7176
a I b Fax; 828.252.4618
VW
It_:luent Aquatic Toxic-t y'jteporTwt°Qrm/Phase If Chronic Cerrodaphnia
+* Facility Raleigh NPDES#:NC 0082378 pipe# 1002 Co
Laboratory Performing Test PACE An 1 suv ices . '- ents; County Wake
Comm
841146lure of opwalor In R88phAsl; Cnema
�
a>4r+a of
b 5voe
Selmnle Infarmatlon
76
Semple 1
Sample 2
Centre)
TsSF
a`�tMID
End Q�`q Stan Time
Collection Stan Date
11114W5
11/17105
fa t'on�
�11�s`0s
'+tzs�os ta:so
Grab
Start
Renews Renew2 Start Renrrtal
Composite (Duration)
2ghr
25hr
Treatment 100 y
100 7 100 '� Control Control
Hardness (mg/E,)
pH Initial
6.9
6.8 6.t3 7.6 7.4
Spec. Cond. (µmhos/cm)
214
220
48
1 pH Final
6.8
6.7 7.1 7.7 7.8
Chlorine f mg/L)
c0.1
70.1
:RRTemp.
130
D.O. Initial
8.0
7.8 8.5 8.1 7.9
Sample Temp. 8t Receipt
0.0 C
2 C
n.o. Final
7.9
7.7 8.3 7.9 7.8.
lnffial
25.4 1
25.0 25.3 24.7 i 24.6
Temp, FInall
25.1
F 24.9 2A.S l 54 7 t 2^ to '
Organlsin 4
# Young
1 2 3 4 5 6 7 8 9 10 11 12 mean
Control
24
18
21
22
25
P.8
19
24
2P..
22
NA
NA 22.5
'm►
adult
(t.)Ive (D)iad
L
L
L
L
L
L
L
L
L
L
NA
NA
# Y°ung
1
2
3
4
5
6
7
8
9
10
11
12 moan
" Effluent%
23
26
21
P24
22 1
28
0
22
25
NA
NA
19.2
22.ti
Adult
(L)TV9 (o)oad
L
L
L
L
L
L
D
L
L
NA
NA
Uad
6.2
# Younp
1
2
3
4
5
6
7
8
9
10 mean
@St R its
7.1
0
_8.4
8.2
24.8
24.8
% Control with 3rd Brood 100
Control Reproduction CV 13.0
48 Hour Mortality
w
Q o 10 0 o 10
Significant? Nb
Final mortlatlty sloni8can!'4t na wens
Effluent°Ia 22 26 7_7 30 26 25 0 2 27 2S 23.1 Reproductlon Analysis:
4,5
Adult
(Qlve (o)and
L
L
L
L
L
ten
t�v
L
D
L
L
L o.0
Rgprrsduclion LOECa
s100 % : NOEC= 100 %
#Younp
1
2
3
4
6
6
7
8
9
10 moan
Method:
Steel's Many One Rank
Effluent%
24
24
22
23
23
24
24
2
24
29 24.6
Normal Dlstrib7
No Mothed: Kotrnogorav a
7E
Adult
Wive (D)nad
L
L
L
t
L
L
L
L
L
%Red
L 0.0
Statistic:
1.634 Critics;: 1.035
� Younq
1
2
3
4
5
6
7
8
9
10 moan
N -PargmatiaanalysLisr(if
applicable):
Effluent%
23
27
25
29
27
26
6
3
33
28
27.3
Method:
90 (L)tvo (D)aad
L
L
L
L
L
LL
E
L
L
L
o o
E ffluent%
Rank Sum GdUcai Sun-;
� #Younp
1
2
3
4
5
6
7
$
9
10 mean
22.8
----}-
108.6 75.0
affluent%
16
21
19
13
23
23
23
23IL
i
NL
20 ,
5
1�5 75.0
100
Adult
(Olve (waaa
L
L
L
D
L
L
L
L
�8 s
`
75
19A 5 75 0
MAIL ATT: Environmental Sciences Branch ' Should Us
�90
b highest lest '
10000
144.5 75.0
-
9^--1�-5
Div. of Water Quality
_ TO'
contnntratlon
or Honest
7 1
N.C.DENR
conrentrauort
P41h b.o. 1-00 mq/L Overall Analysis:
� 1621 Mail Service Center
Raleigh, NC 27699-1621
-• %Rvduc
Anpmducllon
lion From Control Result - PASS/FAIL
Test LOEC= �
Mean 100
or
o 0
/o ; NOEC= 1oo.0 /o
Chronic Value =
>1oo °fo
Asheville CerilOCatfon IDs
NO Wastewater 40
REPORT OF
ABQRATQRY AN
Mari * %stiUrS,
NC Drinking Water 37712
This report sh
11 not be reproduced, except in full,
NC 12
SC 99030
withatlt the written
.onsent of Pace Analytical Services, Inc.
Drinking W
Ii)C Drinking Water 37706
FL NELAP E87648
Q ,,:..��o,,��
SC 99006
�R�e�c
FL NELAP E87627
lum

Grub sample
-ate: Time:
m
ar
m
N
i
m EnIARIts:
m
t
m
a' AMPUCONDITION
Received on lee y/ r Pit
o 'ealed Cwler
N )amplts Intact y
'roject Number: Qq 7
m :ample Nmttber. qa (Q 3 %G
N I J
v
.+ s
OI
N AQUATIC TO7iICOLt)GYLABOi ATORy
N CHAIR-OF-CUMD 17 AND FIELD ANALYSIS P4)RM
Tha Chain-Df-Custody is a LF:ML DOCUMENT. AU ielevaul fie::te must be completed accurately.
a.
TEST REQUIREPIENI'S (pleas;: check the appmPriate test type and species required.)
Trs/ Tjpe ++
J _._ Ac¢1F Fircle the appropriate lest durafiou: 2q 46 96 _
a Chronic, T-do}•s
Pass/FaR, dilnlion rey¢ired:
aF¢R Range DeRttitive, d8utiooa rrq¢hed: �Op�9py-7�y� 2,Z ,rj"
3 Othn; specifv-
Z
O
Z iAMPLETYPE
= Corn o st 'te I
aceAnaloical
Specief
Fzi—cefiodaphrl dabia (Water Rea)
pauepbafrs prmaejar(Fathead minnuu)
,4lvaidopsis bahfa (Mysid shrimp)
bfmidra beu&ita ([mood silverside)
p p ramp a Sample Iufm-matiou
'tat=date- %i-Iy+05 Start time: Ma — OTOO Sample location: (afterchlofinstion, aboveoutfaR, at weir, effluent outfnll, etc.)
w and date; JJ-(S-OS Endtime: O't:nD
iampks per hour. F'JOW Pao 'rlbtud Approximate volume collected for testing: 3 L.
-1111e dmiug rnllettioo?: 7E5 Number-ofcontainersfined for testing: ?) ---- - --
f chiQed, specify- tempaat¢tx: 3.8 Total residual chlorine (utg/L): —
Method of trauaport to PACE: -_
PACE ANALYTICAL SERVICES, INC.
2223 RIVERSIDE DRIVE
ASHEVILLE, NC 288D4
PHONE: [phone]
FAX: [phone]
14652
.....v...,....,.�.•�. �n�., ............
Samples must be received at PACE at or below 4°C. Samples should twl be ffnun
Signature: i5fLpia4, IDate/Time: fJ -f5-05 (� Bq:20
1hS/1.]I[iXliYl}1��, f� .j: m / 'li/lf���

l 7 1 I I 1 1 I 1 I 1 1 1 i
+ AQUATIC TOXICOLOGY LABORATORY
CHAIIN-OF-CUSTODYAND FIELD ANALYSISFORM aceAnalytical
The ChaiFI-Custody is a LEGAL � DOCUMEA'1'. All relevant fields u3ust be completed accurately. ,
Poo to14 Zq Cr.
THIS FORM IS REQUIRED RS'
TEST IIEQUIREAIENTS (Please check Lbe appropriate lest type and species required.)
Tee! Tjpr
r— Acute, circle the appropriate test duration: 24 48 96 Spicier
a�Chronic, 7.days ✓ Cerrndwimladubia(Waterf")
_ Pass/Fail, dilution required: PW1WPirder pronwbn(Fathead mianow)
Mfoidopsis ballia (M sid shlirup)
r Full Range Uctirrifivq dilations rYquired: 1bp,tjM "75 try
3_Olher; apecifi': ,22.5- dfeaiatia begJ/nra(Inland silverside)
ZSAAIPLE TNPE
O Composite, sample
�>tar( date: /I-/'J. c;S- Slatme:
LdateExul miomples per boor. F=%w rproporf
ihilled during collection?: ke�—S
f chilled, sperifi' temperatuie: 3, 7
i
m
PACE ANAMMCAL SERVICES, INC
2225 RIVEESDjF.DRIvr
ASBEVILLE, NC 28804
PHONE,: [phone]
FAX: [phone]
10039
sample Information
SemPle location: (after chlorination, above outfall, at u-eir; effluent outran, etc.)
o02 EWIuCn-F"
Approximate volume mllected for testing:
Nu mher of conlainers fd?ed ftrr lesdng:
Total tesidual chlno,.. r..,.,n i-
semperamteat lime otcolleclipa cc)
Grub sample Method of tramport to PACE: —
CAla.r i P r
late: Tiub e: Note: Rinse containers with the sample before fdling. Pack samples is loose ice.
Samples must be received at PACE at or below 4'C. Samples should not be frozen.
or AMPLE CONE
Received on Ice
y;ealed Cooler
N iomples intact
inject Number.
m;amplc Narnber:
v
N
ti
.11
RELINQUIRftT'D HY RaTI[ Mir A('C'Ef'IfO GY L`:SI'f- 'flRl?;
❑mp.. 7eml. C'