NC0024911_Effluent Toxicity Report_20221222

Action History (UTC-05:00) Eastern Time (US & Canada)
Submit by Anonymous User 12/22/2022 8:58:49 AM (Supplemental Submittal)
Submit by bradley.bennett 6/26/2023 4:04:49 PM (Supplemental Info Submittal)
• The task was assigned to Thedford, Wren, Bennett, Tilman B 12/22/2022 8:58:52 AM
• bradley.bennett assigned the task to bradley.bennett 6/26/2023 4:02:42 PM

Initial Review Information
.........
Updated ID#: * IMPORTANT: REVIEWER SHOULD VERIFY and revise here if necessary.
NCO024911
How should this project be handled? *
File Discard
Information Submitted on 12/22/2022
Submitter Information
Name: Bart T. Farmer
Who is submitting the information?
Email Address:* [e-mail]
Submittal Information
The Division of Water Resources (DWR) is providing this site to allow for the upload of required supplemental information
associated with discharge monitoring reports (DMR). All DMR data is required to be submitted electronically through
the eDMR system and should not be submitted here. Division staff will review all uploaded documents and will only
maintain required information. Documents such as laboratory reports are not required to be submitted and will not be
maintained in the system.
Project Information:
Please supply the permit number for this project.
...............................................................................................................................
Existing ID#* Permit Number
NCO024911
Examples: NCxxxxxxx or NCGxxxxxx
Facility Name: * French Broad River WRF
County: Buncombe
Please upload all files that need to be submited. *
Click the upload button or drag and drop files here to attach document
MSD_NCOO24911—November 2022 Toxicity
1.53MB
Report.pdf
Only pdf files are accepted.
Please provide descriptive comments about the attached information.
Attached is MSD's November 2022 Bioassay Toxicity testing. These results are also reported on our monthly eDMR
report for the month of November 2022.
* By checking the box and signing box below, I certify that:

• I have given true, accurate, and complete information on this form;
• I agree that submission of this Supplemental Information form is a "transaction" subject to Chapter 66, Article 40 of the
NC General Statutes (the "Uniform Electronic Transactions Act')
• I agree to conduct this transaction by electronic means pursuant to Chapter 66, Article 40 of the NC General Statutes
(the "Uniform Electronic Transactions Act');
• I understand that an electronic signature has the same legal effect and can be enforced in the same way as a written
signature; AND
o I intend to electronically sign and submit the Supplemental Information form."
Full Name: Bart T. Farmer
Signature:
cY�rl-lt ��ILt�I!'�it
Date Submitted: 12/22/2022

Metropolitan Sewerage District
• '�`--� OF BUNCOMBE COUNTY, NORTH CAROLINA
2 %%'
c°MB '. aPua se1o�1�� GPQ
6 C�UNTY. 140P'
December 22, 2022
Environmental Services Branch
NCDEQ-Division of Water Resources
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
Dear Sir/Madam,
Attached are the results and supporting information of the Fathead Minnow Survival and Growth test
and Bioassay Toxicity testing for the week of November 8, 2022, for the Metropolitan Sewerage District
of Buncombe County (MSD). This is the Fourth and final required chronic pass/fail toxicity tests in 2022.
Indicated on page nine (9) of this report, the bioassay toxicity results of this report are "Pass." Indicated
on page fifteen (15) of this report, the results of the Fathead Minnow Survival and Growth test were
>48.00%. These results are also reported on our monthly eDMR report for the month of November.
If you have questions concerning this report, please feel free to contact me.
Sincerely,
Bart Farmer, Director
Operations & Pretreatment Division
Office (828)-225-8224
[e-mail]
Protecting Our Natural Resources-
2028 RIVERSIDE DRIVE, ASHEVILLE, NORTH CAROLINA 28804 TELEPHONE: [phone] FAX: [phone] WEBSITE: www.msdbc.org

a
laceAnalXical
www.pacelabs.com
December 21, 2022
Bart Farmer
MSD of Buncombe County
RE: Project: NC002491111/8
Pace Project No.: 92635344
Dear Bart Farmer:
Pace Analytical Services, LLC
2225 Riverside Dr.
Asheville, NC 28804
[phone]
Enclosed are the analytical results for sample(s) received by the laboratory between November 08, 2022 and November
10, 2022. The results relate only to the samples included in this report. Results reported herein conform to the applicable
TNI/NELAC Standards and the laboratory's Quality Manual, where applicable, unless otherwise noted in the body of the
report.
Some analyses were subcontracted outside of the Pace Network. The test report from the external subcontractor is
attached to this report in its entirety.
The test results provided in this final report were generated by each of the following laboratories within the Pace Network:
If you have any questions concerning this report, please feel free to contact me.
Sincerely,
0
Taylor Dyson
[e-mail]
[phone]
Project Manager
Enclosures
cc: Ms. Shannon Bergeron, Metropolitan Sewerage District
Mr. John Gosnell, Metropolitan Sewerage District
Ms. Sheila Kilby, MSD of Buncombe County
Mr. Chad Ledford, Metropolitan Sewerage District
Ms. Angel McCrain, MSD of Buncombe County
Patrick Smith, MSD of Buncombe County, NC
Mr. Alan Taylor, Metropolitan Sewerage District
Mr. Schuyler Taylor, Metropolitan Sewerage District
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
l without the written consent of Pace Analytical Services, LLC.
Page 1 of 7

DC#_T1t1e: ENV-FRM-HUN1=0083 V01_San1pie Condition Upon Receipt
Neptive Date: 06/12I2022
Laboratory re elvin . samples,-
Ash6V1lje Eden[? ereoftood ❑ Htlntem.111140 Rayleigh[) Mechalni;csVllllen Atla ltai -I KeYnersviupl-1
Will -i Client Name: Project WO# 92635344,
tf;
Coyne,-; E]Fed.EX []UPS❑USPS cirent
b Commeecial []Pace [1pthek: 82836344
t
Custody Seal. Present? nYes OK9 Sgats MOO OYes 290
Date/Initlals Person exatnlnrng Contents•, 2
packlogMaterlah []bubble Wrap npubble•8ags Obnd El, other BiologicalTlssue kozeo?
Thermomek- ' ❑Yes ❑No ❑N/A
1 R Gun ID: 7 t `.et .QBlue,
'Cor(ection Factors Type of Ice: P
CoolerTemp: S Add/Subtract(°Cj .
C:oolerTerhO Corrected] �, G
USDA'Regufatdd Sofl N/A, water sbmPip)
[]None
Temp should be above freezing to 6T,
EIS.amples'out of temp criteria, Samples on Ice, cooling process
has -begun
Dln5ampl'e5 QClgand(e tr1.d gttar�7ntlne
(check maips)?,[]Yes ONo
zone wants) [ne unlLep Grates: cy, IVr,or?�
'
uta samples onginite rrgma tutet�n;uurce tntremauunany,.
Including Hawaii and Puerto.Rico)?,[l 6s E�JNo
_ . _ .
Commhnts/bisZ+rdpancy:
Chain of Custody Present?
25yes,
[:]No
CINIA
1.
Samples ArrlvO wt(hfn Hold Time?
es
QNo
QN/A
.
Short H-ald Time Anai sls (42 hr.)?
es
(]No
[]N/A
3.
Rush Ttarn,Around Time Requested?
Yes _
'o
ON/A
4,
Sufficlpn4Volume7
Yes
No
N/A
S.
Correct 6nwl,ners Used?
.Pace Containers Used?
Yes
Ds_
[INo
[]No
[jN/A
[jN/A
6.
ContalnerS.lnta@t?
bYes
❑No
[]NIA
7.
Dissolved gp5lysls: Samples Field. Filtered?
[]Yes
No
/A
8,
sample Labels Match COC?
-Includes Date/Time/ID/Analysis Matrix:_
es
%
[)No
[INlA
9:
Hesd�paceInVUAVIais(>5.6mm)?
[]Yes
[]No:
N/A
10.
Trip Qlank Presennt?
Trip Black Custody Seals Present?
[]Y•es
[IYes
[]No
No
A
11,
COMMENTS/SAMPLE RIKKEF'ANI V
Lot ID.,of spilt cobtalners:
CLIENT NOTIFICATION/RESOLUTION
Person contacted:
Project Manager SCURF Review:
Project Manager SRF Review:
Date/Time;
Dater
Date:
r,eiu uma neyuucur Urea Urvu
Page 2 of 7
Orlaltrax Ins 69614 Page 11 of 2

DO#;�7itl'p: ENV-pRM-HUN1-g083 vol_$aimple Condition Upon Receipt
Effective Oafs: Q5/12/ 62
#Check mark tap hate of box If pH and/or clechIcIripatign is vet fied.and project #
Within the acceptance range for preservation samples,
Except►onsi VCA, coliform, j'OC, Cill and Grwo, 0. 60615 (water) DOC, LLHg-
"*8ottom,half ofbox'Is.to list nufnber of bottles
***Check a (I unpreserved Nitrates for chlorine
WO#:92636344.
PM: TOO Due Date: 12/01/22
CLIENT: 93—MSD
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pN upon receipt
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Lot N
Note: Whenever there is a discrepancy affecting North Carolina compliance samples, a copy of this form will be sent to the North Carolina DENR Certification Office (Le,
Out of hold, Incorrect preservative, out of temp, Incorrect containers.
Page 3 of 7
Qualtrax ID: 69614 Page 2 of 2

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DOLT-ifle; ENV-FRWHUN1,0083 V01 SAMOle Gondition- Upon ftcelpt
EffeOlvit DaW 06MV2,022
Laibpratdry !L;-Jetrim samples:
gdeqEl OreonWood F] HmntetwIlle.
n RoJelghn Meehan k's 111en At[ahtaQ KornersyllIeF1
Sample C ondition I Client Name,-
IJPon Re ceipt�� Prqjdct #; WO#:92635344,
P14: TOO Due Date: 12/01/22
DFod Ek Flops Flusps. ErPlont CLIENT: 93-MSD
[Pate notheh
Custody sea I Present? E]Yes 'ONo Seals Intact? [Yes No
PeckIng M'aterlalc E] pubbk Wrap OBQbblo Bags QN8nd E3, Other
Thermometer;
I R Gu , n ID: q-3 Tt 7 Type of Ice: PVei [)Blue
'Correction Factor;
Coble(Temo: Add/Subtract ('C) 0
C661drTerhp Corrected N/A,
watees�rripfq) usDA-Rogulated Sall (
Old sqmpigs originate In,agparantine zone, within the United States: CA, NY, or SC
b4iie/l,nitIalls Person Examining Contents,
Biological Tissue Frozen?
[]Yes E1Nq-QN/A
ONon'e
Temp should be above freezing to WC,
Elsample'stutiftemp criteria. $Ample,s on Ice, cooling process
ha' begun
D(4 samples originate from a foreign source (IntRrnation@IIV,,
check mips)? UYes Na
inciming nawaii dou rvefw- n1wil Tea. L-Jr4V
Commients/DkedpancV.
Chain of Custody Present?
P Yes,
[]No
EINIA
1.
S;mpJe% Arrived within Hold Time?.
as
No
QN/A
2.
Short Held Time Analysis (<72 hr.J?
es.
[]Nq
[JN/A
3.
Rush Ttmrn,Around TiMe Requested?
QYes
QN4
EIN/A
4.
SufficlentVolume?
QYes
[]No.
[]N/A
5.
Correct Co;itpl.ners Used?
.Pace Containers Used?
•Ejye,
Yes
0N4, qN/A
[IN. QN/A.
6.
t3N.
[JN/A
.7.
Dissolve!c! ap6lyVs; Sarnplds Field Filtered?
E]Yes
ONo
f141-k-
8.
Sample Labels Match COC?
-Includes Date/Time/ID/Analysts Matrix;
QYs
/,A-
[(No
[]N/A
9.
Heacipppce In VOA VIaLs (>5-6mm)?
[]Yes[]Nd,
DNIA
10.
Trip olaFik Present?
Trip.slainkCustody Seals Present?
[]Yes
[]yes
[]No
QNo
C101A
N/A
III.
COMMENTS/SAMPLE DISCREPANCY
Lot ID of split containers;
CLIENT NOTIFICATION/RESOLUTION
Person contacted:
Project Manager SCURF Review:
Project Manager SRF Review:
Date/Tlme:
Date:
Date:
rielaudidnequirCOF L.Jit!!. L_J[4u
Page 5 of 7
oiialtra)( in- 69614 Paqe 1 of 2

'Che&-maiek top half of b6x,If PH 4qd/qrdochlq!Inatl I on is
s vet l - ftqOAncl
within the aciceptanco range for preservation samples,
excep,064sl VGA, coliform TCrC, Oil and Grease, O-ROA015 (.water) . C L Hg
**8,DttQrq half of. box is to list n ' Urqber of bottles.
***Check a IIanpreserved Nitrates for chlorine
130- itlet ENV-FR.M-HUN1,0083 vOI_Sample Condition 0-pon kecelpt
Motive 0511 212022
Project # wUV:9ZbJ5344,
PM: TBD Due Date: 12/01/22
CLIENT: 93-MSD
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Log for Preserved Samples
sample to
Type of Preservative
pH upon receipt
Date preseyvation adjusted
Time preservation
adjusted
Amount of Preservative
added
'Lot 0
Note: Whenever there is aisrrepancy allecting Norm Larclitna compliance samples, a copy of inis io(m will Ue 512111. to the IMitil VO[Vlllld UrINn LCltllli3OUV91 UIPILlf JIM,
out of hold, Incorrect preservatIve, out of terrip, Incorrect containers,
Page 6 of 7
qualtrax ID: 69614 Page 2 of 2

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Page 7 of 7

E,,,Tf,,, (E. 1) [177 g42 . t rid: (t304) E17� 0��E1
P.O. Box 16414, Creenville„ 0C 29606 4 Coaftsrrlan Cwt, Greet, SC 29650
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: MSD OF BUNCOMBE COUNTY
Facility: WRF
NPDES #: NCO024911
Test Date: 02-Nov-21
Laboratory ID #: T60518 C dubia
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
President
w
Certification #1 87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept, of Health. Included results pertain only to provided sam leg
Rag,e 1 of 6
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification # 022

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 18-Nov-21
Facility: MSD OF BUNCOMBE CO. WRF NPDES# NCO024911 Pipe # 001 County: Buncombe
Laboratory Performing Test: Comments
X
Sig ure of Operator in Responsible Charge �n '
X / g✓
Signature of Laboratory Supervisor
MAIL ORIGINAL TO Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Chronic Test Results
Calculated t= 0.3419
Critical Value= 2.508
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 % Reduction= 1.2%
# Young Produced 22 21 22 18 22 20 23 20 21 22 21 25 % Mortality Avg. Reprod.
Adult (L)ive (D)ead L L L L L L L L L L L L 0% 21.4
Effluent %
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)Ive (D)ead
pH
Control
Treatment 2
Control Control
0% 21.2
Treatment Treatment
Control CV
1 2 3 4 5 6 7 8 9 10 11 12 8.1%
23 19 21 22 18 22 21 24 20 19 22 23 % 3rd Brood PASS FAIL
L L L L L L L L L L L L 100% X
Complete This for Either Test Test Start Date
Collection (_Start) Date 02-Nov-21
1st sample 1st sample 2nd sample Sample 1 3,-Oct-21 Sample 2 02-Nov-21
7.6 8.1 a
.5 7.7 8.3
.6 7.8 8.3
start end start end start end
D.O. 1st sam le 1st sam le 2nd sam le
Control 7.6 8.3 7.9 8.4 8.0 8.6
Treatment 2 7.7 8.4 8.1 8.3 7.9 8.2
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
LC50 = %
95 % Confidence Limits
Organism Tested
Grab
Comp
IDuration
1st
2nd
Sample 1
X
24hrs.
Tox
Tox
Sample 2
X
24hrs.
Dilution Sample
Sample
Hardness (mglL)
85.4
Spec. Cord. (pmhos)
318
527
546
Chlorine (mg1L)
<0.05
<0.05
note Temp. at receipt (°C)
2.3
0.6
Mortality, slartlend starlend
Method of Determination Control
Average ROther
ProbitED High Con..
an Kerber pH D.O.
dubia
DEM Form AT-1
Page 2 of 6

STATISTICAL ANALYSIS RESULTS
Facility: MSD
NPDESH NCO024911
Sample ID: WWTP
ETT# T60518 C dubin
Date: 02-Nov-21
Laborato :
Certification #: NCO22
I Exp. Date: I l/1/2022
Survival Data
7 Day Survival Test Used: FISHERS TEST
Control 100% Test Statistic: P= 1.000
Effluent 100% Critical Value: P= 0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female
Std. Dev.
Test Used: Shapiro -Willis Test:
Control 21.4
1.73
Effluent 21.2
1.85
W:
0.976
Critical Value:
0.884
Analysisfor Differences in Reproduction
Test for Honrogeneitp of Variance
Test Used: Equal Variance
t Test.
Test Used: F Test
Calculated t=
0.34
F=
1.14
Critical Value=
2.51
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
source
rep
H410-21
1
3
+8+11
22
U3 10-22
2
4
+7+10
21
R7 10-21
3
+4+7
11
22
K510.21
4
3
+6+9
18
X510-22
5
4
+7+11
22
N1 10-21
6
4
+6+10
20
B310-22
7
1
1
4
+7+12
23
18 10-21
8
3
+8+9
20
V10 10-22
9
4
+7+10
21
Q510-21
10
3
+7+12
22
D810-22
11
3
+7+11
21
L310.21
12
5
+8+12
25
13
N/A
0
14 INIA
1
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
o
IMean
20
N/A
0
21.4
12 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
H410-2
1
1
4
+8+11
23
U310-2
2
4
+6+9
19
R710-2
3
+4+7
10
21
K510-2
4
+3+8
11
22
X510-2
5
3
+7+8
18
N 1 10-2
6
a
+7+11
22
B310-2
7
3
+8+10
21
18 10-21
8
5
+7+12
24
V1010-
9
4
+6+10
20
Q510-2
10
3
+7+9
19
D810-2
11
4
+7+11
22
L3 10-2
12
4
+8+11
23
0
13
N/A
0
0
14
N/A
0
0
15
N/A
0
0
16
N/A
0
0
N/A
0
0N/A
0
0N/A
r17
o
Mean
0
N/A
0
21.2
JC
JZ
JC
AM
JC
Jz
09-Nov-21
-
07:40 AM
03:56 PM
71:41 AM
05:41 AM
09:27 AM
71:61 Ah1^"
10:15 AM JC
24.7
24.5
Old tamp. °C
24.81
1 25.2
25.1
Control New tamp. °C
24.51
1
1 24.5
L
T60518 C dubia
... %
MSD
W W TP
NCO024911
Buncombe
$rF111
02-Nov-21
ff1 111itg
03:15 PM
JC
Ceriodaphnia dubia
FfR4P1r 1�1fl ?
01-Nov-21
1700-2200
NCCPF
{j,�pnt C
MHSF 10/27/218
G1iTt€arnkw
A
ibs1l(eSBeJ ,,;" .
12
30 ml
15 ml
1
1611/8dk
i t "0.05ml
ia11 ifs�NlssSi _." a ^
24.8
0.05 ml
EPA 821-R-02-013:1002
ini lem
17 AND 60518 SHARE A CONTROL
Page 4 of 6

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EQt€tttta „Irt . (E.64) 877.6942 . FAX (869 ) 9 7-6938
P.O. Box 16414, GreenV9lle, SC 29606 4 Craftsman Courl, Greer, SC 29650
Fathead Minnow Survival and Growth Test
EPA-821-R-02-013 Method 1000
Permit Renewal Second Species Testing
Client: MSD OF BUNCOMBE COUNTY
Facility: WRF
NPDES #: NCO024911
Test Date: 02-Nov-21
Laboratory Sample ID #: T60518 P PROMELAS
Test Reviewed and Approved By:
V
Robert W. Kelley, Ph.D.
President
` Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples.
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #2310a
NCDENR Certification # 022
Page 1 of 6

Effluent Toxicity Report Form -Chronic Fathead Minnow
Facility: MSD OF BUNCOMBE CO.
Laboratory:
NPDES # NCO024911
Jlyl IdLUlC VI IJPUI d[UI III RUJI./VI IWIJIC t IIQI yG W 4 x
Signature of Laboratory Supervisor
MAIL ORIGINAL TO: Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time
11/02/21
/ 03:15 PM
% Eff. Repl.
1
2
Control Surviving #
Original #
Wt/original (mg)
3.0 Surviving #
Original #
Wt/original (mg)
6.6 Surviving #
Original #
WUoriginal (mg)
12.0 Surviving #
Original #
Wt/original (mg)
24.0 Surviving #
Original #
Wt/original (mg)
F--4-8.-ol Surviving #
Original #
Wt/original (mg)
4 4
Pipe #: 001 County:
Avg WUSurv. Control 6.6845
10
10
9
9
10
10
10
9
0.6460
0.6110
0.6440
0.7656
10
10
1 10
9
10
10
10
9
0.6660
0.6180
0.6630
0.6744
10
8
1 10
1 10
10
9
10
10
0.6880
0.8333
0.6770
0.6660
10
10
1 10
9
10
10
10
9
0.6960
1 0.6340
0.6880
1 0.4889
10
10
1 10
9
10
10
10
9
0.7150
1 0.6630
0.6530
0.7622
10
10
1 10
10
10
10
10
10
0.7750
0.7320
0.6830
0.7850
% Survival 97.4%
Avg Wt (mg) 0.6666
% Survival 100.0%
Avg Wt (mg) 0.6554
% Survival
Avg Wt (mg) 1 0.7161
% Survival 100.0%
Avg Wt (mg) 1 0.6267
% Survival 100.0%
Avg Wt (mg) 0.6983
% Survival 100.0%
Avg Wt (mg) 0.7438
Test Organisms
PXCultured In -House
Outside Supplier
Hatch Date: 11/01/21
Hatch Time: 12:00 AM
Water Quality Data
Day
Control
0
1
2
3
4
5
6
7
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) IniUFin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
7.8
I --
7.8 /
7.6
7.7
/ 8.1
7.8 I
7.8
7.8
/ 7.7
7.8
/ 8.0
7.8 I
7.6
---- I
7.7
7.6
/ --
7.3 I
6.1
7.9
/ 7.2
7.5 I
6.2
7.5
I 6.4
7.4
/ 6.5
7.5 I
5.9
---- /
7.5
24.7
/ --
24.5 /
24.9
24.6
/ 24.8
24.5 /
24.2
24.7
/ 25.5
24.8
/ 25.4
24.6 /
25.6
---- /
25.5
n 1 9 R d F R 7
I -�/6.4
/
8.0
8.3 /
7.8
7.7
/ 7.7
7.9
/ 7.8
8.0
I 7.6
---- l
7.6
P8./ -
/
7.3
7.1 /
6.1
8.1
/ 6.0
6.5
/ 5.8
6.4
I 5.5
---- /
5.5
25.0 / -
24.6 /
24.9
24.7 /
24.8
24.6 /
24.2
25.1
/ 25.5
24.9
/ 25.4
25.2
/ 25.6
---- /
25.5
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
1 2
10/31/21
11/02/21
11/04/21
24 hr
24 hr
24 hr
68.8
85.4
187.5
0.0
0.0
0.0
527
546
572
1 <0.05
1 <0.05
<0.05
1 2.3
1 0.6
3.2
Dilution H2O
Hardness (mg/L) 85.4
Alkalinity (mg/L) 1 58.2
Conductivity (umhos/cm) 318
Survival
Growth
Normal
no
yes
Hom. Var.
yes
yes
NOEC
48.0%
48.0%
LOEC
>48,00%
>48.00%
ChV
>48.00%
>48.00%
Method
Steel's Test
T-Test
Overall Result
ChV >48.00%
Stats
Survival
Growth
Conc.
Critical
Calculated
Critical
Calculated
3%
10.00
20.00
2.41
0.25
6%
10.00
17.50
2.41
-1.08
12%
10.00
20.00
2.41
0.87
24%
10.00
20.00
2.41
-0.69
48%
10.00
20.00
2.41
-1.68
DWQ Form AT-5 (1/04)
Page 2 of 6

MOM
. a
©--------------_-_�
Lab#
T60518 P PROMELA
Client
MSD
Sample ID
WWTP
NPDES#
NCO024911
County
Buncombe
Month
11
Start 8 fed Date
02-Nov-21
Start & fed Time
03:15 PM
Started 8 fed By
AM
Test Organism
Pimephales promela
NOD. born date
01-Nov-21
NOD. born time
1130.1300
Test Type
NCCD
Dilution Water
MHSF
Units for Cone.
%
%3rd BROOD
Test vessels
400 ml
Testvolume
150m1
Incubator#
1
Light
161U8dk
Initial Temp °C
25
Artemia
.015g MAY
Test method
EPA e27-R-02-019',1e00
repshave 10
is unless noted otherwise.
Page 3 of 6

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