NC0021954_Effluent Toxicity Report_20220309

Action History (UTC-05:00) Eastern Time (US & Canada)
Submit by Anonymous User 3/9/2022 9:54:59 AM (Supplemental Submittal)
Submit by bradley.bennett 6/26/2023 4:03:57 PM (Supplemental Info Submittal)
• The task was assigned to Thedford, Wren, Bennett, Tilman B 3/9/2022 9:55:02 AM
• Bennett, Tilman B assigned the task to Bennett, Tilman B 12/20/2022 10:43:36 AM
• Bennett, Tilman B released the task 12/20/2022 2:42:27 PM
• bradley.bennett assigned the task to bradley.bennett 6/26/2023 4:02:42 PM

Initial Review Information
......... ......... .......................................................................................................................................................................................................................
Updated ID#: * IMPORTANT: REVIEWER SHOULD VERIFY and revise here if necessary.
NCO021954
How should this project be handled?
File Discard
Information Submitted on 3/9/2022
Submitter Information
Name: Hal Hardison
Who is submitting the information?
Email Address:* [e-mail]
Submittal Information
The Division of Water Resources (DWR) is providing this site to allow for the upload of required supplemental information
associated with discharge monitoring reports (DMR). All DMR data is required to be submitted electronically through
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maintained in the system.
Project Information:
Please supply the permit number for this project.
.............................................................................................................................................................................................................................................................................................................................................................................................
Existing ID#* Permit Number
NCO021954
Examples: NCxxxxxxx or NCGxxxxxx
Facility Name: * CITGO Petroleum Corp. Selma NC
County: Johnston
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Click the upload button or drag and drop files here to attach document
Selma2022EffluentToxicityReport.pdf 1.71 MB
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* By checking the box and signing box below, I certify that:
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• I agree that submission of this Supplemental Information form is a "transaction" subject to Chapter 66, Article 40 of the
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• I agree to conduct this transaction by electronic means pursuant to Chapter 66, Article 40 of the NC General Statutes
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Full Name: Hal Hardison
Signature:
Date Submitted: 03/09/2022

Effluent Toxicity Report Form- Chronic Pass/Fail and Acute LC50 Date 2/2/2092
Facility Outfall 00- NPDES#NC 0021954 Pipe # 001 County Johnston
Laboratory Performing Test Pace National Comments Pace National ID:
x L1456929-01
Signatur f Ope or 'n Responsible Charge
x 2-22 2
Signature of(Laboratory d6upervisor
Environmental Sciences Branch
MAIL ORIGINAL TO:
Div. of Water Quality
N.C. DFNR
1621 Mail Service Center
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test Raleigh, North Carolina 27699-1621
CONTROL ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12 Chronic Test Results
—n ICalculated t
# Young Produced ° /o Tabular t
Reduction
Adult (L )ive (D )ead
Effluent%
TREATMENT 2 ORGANISMS 1 2 3 4 5 6 7 8 9 10 11 12
# Young Produced
Adult (L )ive (D )ead
list sam le 1 st sam le 2nd sample
Control
pH
Treatment 2
s s s
t e t e t e
a n a n a n
r d r d r d
t t t
1st sample 1st sample 2nd sample
Control
D.O. FE
Treatment 2
LC501Acute Toxici Test
(Mortality expressed as %, combining replicates)
Treatment 2
Control CV
% control organisms
producing 3rd brood
Complete This For Either Test
2
PASS FAIL
Check One
Start Date
Collection (Start) Date
Sample 1 2 I 1 1 2 2 Sample 2 I 1
Sample Type/Duration
Grab
Comp
--
`= o
nX
Sample 1
grab
N/A
rDur2ation
x
(
T),
--------
O
�3
mSample2
0
m
�m
To
%
%
5d
%
75
%
100
%
%
%%
Q
12.
25
%
%
%
%
%
%
%
%
%
0
0
0
0
5
14%
LC50= > 100 %
95% Confidence Limits
Method of Determination
Moving Average ❑ Probit ❑
Spearman Karber ❑ Other
Hardness(mgll) 190
Spec.Cond.(pmhos)1 332 123 .2 1 N/A
Chlorine(mgll)
Sample temp. at receipt
Concentration
Mortality
Organism Tested Pime hales 1promelas Duration (hrs) 24
DWQ form AT-1 (3/87) rev. 11/95
0.00 1 N/A
5.4 1 N/A
Note: Please
Complete This
Section Also
start/end start/end
7.7F761 Control 8. 9FBol
7.1 6.9 HighConc. 8.5 7.5
pH D.O.
Page 1 of 9

Reviewed by: t i
aceAnalyt-ical' Control Water (Tank ID): _ ,� 0-1
r
hlo ' nal Center for Testing & Innovation Control Water (Begin Use Date): r4 -
i
client: CITGO Petroleum Corp. - Selma Terminal (Outfall 001)
Test start Date: 9 —" q ;� Fac ID or NPDES #: NCO021954
Thermometer Serial #: 18050064
Pace Sample #: I L i q -
Measurements taken directly in test chamber. Temperatures recorded in degrees Celsius.
Effluent Conc (%)
CONTROL
12.5
25
50
75
100(PL)
Fathead Minnow
Fathead Minnow
Fathead Minnow
Fathead Minnow
Fathead Minnow
Fathead Minnow
CONTROL
100% Effluent
Analyst: Ld
Analyst:
Analyst: a
0 hrs
24 hrs (morning)
24 hrs (final)
oc
05,0
-c
9
-C
�c
015.1
c
a q.y
-c
oc
01)5,0
C
9s.0
oC
•
�.1.{ � -Cl
D
oC
a q.
-c
L2'-ft(q �C
.3(
-C
% ��
4.
oC
Total Cl2 (mg1L)
Alkalinity (mg/L)
Hardness (mg/L)
< 0.2
Dissolved Oxygen: YSI 5000 DO Meter/Probe (serial #01 L0435)
pH: Beckman 390pH/Temp/mV/ISE Meter
Conductivity: Thermo Orion Model 150A+
pH/RDO/Conductivity: Thermo Scientific Orion VersaStar (serial #V 02105)
Water Bath: Lindberg/Blue, Model WB1140A-1 (serial #S01 M-580360-SM)
Temperature: Thermometers calibrated to NIST certified thermometer
Alkalinity: Lachat
Hardness: Lachat
Total Residual Chlorine: Hach Pocket Colorimeter, Model #DR300 (serial #19110A002361)
Environmental Chambers: 25 degrees C + 1.0 degree - Thermo-Kool
Environmental Chambers (for Colorado tests): 20 degrees C ± 1.0 degree - Thermo Scientific Model 3759
Light Quality: Ambient Lab Illumination
Light Intensity: 50-100 ft-c - VWR Traceable Dual -Range Light Meter- Model 62344-944 (S/N 210158976)
Photoperiod: 16 hours light, 8 hours dark
Drying: Overnight at greater than 60 degrees Celsius in a Fisher Scientific Isotemp Oven, Model 655F
Mean Dry Weight: Determined using Mettler Toledo Balance, AT261 Delta Range
Reference Weights (Set#1): Class 1, TREOMNER, Inc., serial number 85035
Reference Weights (Set #2): Class 1, TREOMNER, Inc., serial number 67812
EPA Acute Manual Edition and Date: EPA-821-02-012 October 2002, Fifth Edition
EPA Chronic Manual Edition and Date: EPA-821-R-02-013 October 2002, Fourth Edition
This method is performed only by Assistant Biologists, Biologists, and Senior Biologists that have
experience with aquatic toxicity testing. Laboratory Technicians, Chemists, and any other
laboratory personnel that are not experienced with toxicity testing will not handle test organisms
during a toxicity evaluation. Lab Techs, Chemists, and others may assist (under supervision) with
the gathering of data during the evaluation (pH, DO, conductivity, alkalinity, hardness, etc.), but
will not be allowed to do any work with the test organisms themselves. The following analysts
have met Technical Training Qualifications and their initials (in parenthesis) can be found on the
bench sheets in this report: Brandon Etheridge (BE); Cody Medley (CM);
Clarissa Moore (CGM); Nadiar Yakob (NY); Emily Novick (EN); Anthony Grist (AG);
Makayla Graham (MJG); Lwiindi Mudenda (LM); Manar Kolta (MK); Hunter Holden (HH);
Mike Lowe (ML); Heidi Buckett (HB); Nathan Hawkins (NH); Ashwaq Albeladi (AA);
-01
Client ID: CITGO Petroleum Corp. - Selma Terminal (Outfall 001)
Page 2 of 9

Chad Deylc� Used in
Crownionka Walk -In
Incubator_.
Dickson (small chart)
Page 3 of 9

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CETIS QC Plot
Report Data: 12 Jan-22 08:56 ( 1 of 1)
Control Chart for January 2022 Acute C.dubla Reference Toxicant Pace National
Test Type: Survival (48h) Organism: Ceriodaphnia dubia (Water flea) Material. Potassium chloride
Protocol: EPA18211R-02-012 (2002) Endpoint: 48h Survival Rate Source: Reference Toxicant REF
CoMmi Chart for January 2022 Acute G.dubla Reference ToxIcant
422S
+1s
wwn
-18
-2s
m n c ro m c O o
0
Mean: 0.5973 Count: 20 -1s Warning Limit: 0.5436 -2s Action Limit: 0.4899
Sigma: 0.06374 CV: 9.00% +1s Warning Limit: 0.6511 +2s Action Limit: 0.7048
Quality Control Data
Point Year Month
Day
Time
OC Data
Delta
Sigma
Warning Action Test ID
Analysis ID
1 �2021
Feb
3
0:00
0.6156
0.01827
0.34
002176-0237
08-9882-7317
2
3
0:00
0.5
-0.0973
A.811
(-)
15-3863-0925
14-3861-7721
3
Mar
17
0:00
0.483
-0.1143
-2.127
(-)
(-) 08-7439-7084
05-2793-4472
4
17
0:00
0.6164
0.01908
0.3551
10-4333-5406
02-5030-4577
5
Apr
7
0:00
0.5596
-0.03769
-0.7013
02-1434-2146
15-2711-1915
6
May
5
0:00
0.6373
0.03998
0.744
04-8855-0129
07-6096-7752
7
19
0:00
0.6391
0,04182
0.7783
01-6240-2222
11-2393.4603
9
Jun
17
0:00
0.6426
0.04533
0.8436
1MO64-0438
11-3675-6294
10
17
0:00
0.683
0.08572
1.596
N
04-8649-4823
19-5272-1855
11
30
0:00
0.6156
0.01827
0.34
01-8534-9999
19-3925-0400
12
Jul
14
0:00
0.6515
0.05425
1.009
(+)
00-0839-0804
10-3368-2505
13
Aug
19
0:00
0.5176
-0.07967
-1.482
(-)
12-4976-0061
00-6404-9763
14
Sep
9
0:00
0.5743
-0.02295
-0.4271
07-8533-6370
08-3330-9392
15
Oct
13
0:00
0.5779
-0.01941
-0.3612
01.4397-9696
15-2906-7102
16
Nov
3
0:00
0.6598
0.06245
1.162
(+)
15-0569-9951
16-8401-1270
17
18
0,00
0.5612
-0-03607
-0.6712
08-2948-1489
19-6311-3e47
18
18
0:00
0.5859
-0.01136
-0.2115
1M026-5074
04-0971-2264
19
Dec
1
00
0.5946
-0,002696 -0.05018
05-3729-7699
15-6948-6653
20
2
0:00
0.5946
-0.002696 -0.05018
17-1546-7488
05-9082-M19
21
2022 Jan
5
0:00
0,6598
0.06245
1.162
{+)
1645842-7466
054755-7777
12065 Lebanon Rd January 2022
s Mt. Juliet, TN 37122
aceanaIxta! Reference Toxicant Test
rrsa:;rrr.�, ^sr; fr to [phone]
t [phone] Fax
001-600-314-4 CETISTM v1.9.4.4 Analyst: P�(p:564

CETIS QC Plot Report Date: 14 Jan--22 09:24 ( 1 of 1)
Control Chart for January 2022 Chronic C,dubla Reference Toxicant Pace National
Test Type: Reproduction -Survival (7d) Organism: Genodaphnia dubla (water Ilea) material: VOtaS51Un1 cnjoriae
Protocol: EPA/821/R-02-013 (2002) Endpoint: Reproduction Source: Reference Toxicant -REF
Control Chart forJanuary 2022 Chronic C.dubfs Reference Toxicant
rN
+2s
+1s
Mean
.1s
.2s
N
O N
N
n
O 02
O G
N - r
r 4 O O O O
Mean:
0.305
Count:
20
-1s Warning
Limit: 0.2267
-2s Action Limit: 0,1483
Sigma:
0.07831 CV: 26.70%
+1s Warning Limit: 0.3833
+2s Action Limit: 0.4616
Quality Control Data
Point Year
Month Day
Time
QC Data
Delta
Sigma
Warming
Action Test ID
Analysis ID
1 2021
Jan
26
0:00
0.2444
-0-0606
-0.7738
19-5629-6226
01-9271-7012
2
Feb
2
0:00
0.2816
-0.02335
-0.2982
09-2062-1182
01-9691-9547
3
Mar
16
0:00
0.4535
0.1485
1.897
(+)
01-3609-3887
10-9817-8578
4
23
8:00
0.2479
-0,05707
-0.7287
01-3138-8964
18-7143-2871
5
23
8:00
0.2234
-0.08163
-1.042
(-)
21-3847-4714
02-2884-6478
6
Apr
6
0,00
0.4256
0.1206
1.54
(+)
03-3313-0925
04-6755-3784
7
20
0:00
0.4171
0,1121
1.431
(+)
14-4052-1330
17-2294-5156
A
May
4
0:00
0.2067
-0-09825
-1.256
{-)
20-8700-98331
03-9275-1593
9
18
0:00
0.3599
0-05492
0.7013
20-7890-6912
12-0158-5941
10
Jun
8
0:00
0.2475
-0.05748
-0.734
02-9180-4810
21-2598-7733
11
8
0:00
0.3362
0-03121
0.3985
12-6453-3465
06-9503-3499
12
29
0:00
0.3288
0-02375
0.3033
10-5750-6124
17-8418-5784
13
Jul
13
0:00
0.3613
0.05627
0.7185
19-0744-3044
08-7751-2570
14
Aug
10
0:00
0.2442
-0.06077
-0.776
14-2389-9415
06-6812-1998
15
Sep
14
0:00
0.3531
0.04807
0.6139
01-5131-7824
04-8510-8962
16
Oct
12
0:00
0.2399
-0.06513
-0,8317
06-5929-2530
0960174-3134
17
Nov
2
0:00
0.3154
0.01035
0.1322
05-4120-7262
13-3177-1849
is
3
0:00
0.3791
0.0741
0.9463
04-1927-5719
15-9146-6216
13
Dec
7
0:00
0.1928
-0.1122
-1.433
(-)
11-5027-8468
15-6723-5131
20
7
0:00
0.2409
-0,06409
-0,8184
12-0264-8899
01-7093-1637
21 2022
Jan
4
0:00
0.2681
-0.03685
-0-4706
02-5537-7434
07-6626-1970
AV 1 12065Lebanon Rd January 2022
aceAnalyi#ical Mt. Juliet, TN 37122
Reference Toxicant Test
(615) 773-i 003
! [phone] Fax
o0i-600-314-4 CETISrm vi-9.4.4 Analyst: Pagakof

CETIS QC Plot Report Data: it Jan-22 16:34 (1 of 1)
Control Chart for January 2022 Acute Minnows Reference Toxicant Pace National
Test Type: Survival (48h) Organism: Pimephales promelas (Fathead Minn Material: Potassium chlo{xie
Protocol: EPAI8211R-02-012 (2002) Endpoint: 48h Survival Rate source: Reference Toxicant -REF
Control Chat for January 2022 Acute hUnnom Reference Toxicant
Y
G
Mean:
0.7985 Count. 20
-1s Warning Limit: 0.6383
-Zs Action Limit: 0.4741
Sigma:
0.1622 CV: 20,30%
+is Warning Limit. 0.9607
+2s Action Limit: 1.123
Quality Control Data
Year Month
Day
Time
QC Data
Delta
Sigma_
Warning
Action Test ID
Analysis ID
_Point
1
2021 Mar
i7
8;00
0.8801
0.08156
0.5028
161780-t888
01-5403-1074
2
Apr
15
0:00
0.7647
-0.03376
-0.2082
01-2811-1480
18v'7-75-5229
3
15
0:00
0.7917
-0.006795
4.04189
02-2898-8093
15-5594-2853
4
May
5
0:00
1.352
0.5538
3.414
(+}
(+) 11-5430-2809
02-70164559
5
19
0,00
0.8785
0.07995
0.4929
18-3528-9340
12-8180-1469
6
19
0:00
0.8485
0.06003
0.3084
01-8129-2223
02-5507-2891
7
Jun
17
0:00
0.7917
-0,008795
-0.04189
20-3082-7283
20-6881-3246
17
0:00
0.8196
0.02112
0.1302
17-5234-5192
18-6913-2513
g
36
MOO-•
10
Jul
14
4:00
0.7917
-0.006795
-0.04189
11-1907-9411
17-9913-5497
11
Aug
19
0.00
0.8485
0.05003
0.3084
12-4704-3267
00-8455-9341
12
Sep
9
0:00
0.8336
0.03508
0.2163
11-4377-8637
17-7413-0465
13
Oct
13
0:00
0.6431
-0.1564
-0.9583
06-9871-8460
06-6481-7453
14
Nov
3
0:00
0.8485
0.05003
0.3084
IM015-1422
17-9725-5266
15
18
0:00
0.7303
-0.0682-2
-0.4206
13-1601-4860
18-4064-4544
16
18
0:00
0.7727
-0.02584
-0.1593
16-3750-5656
15-4927-8397
17
Dec
1
13:47
0.6
-0.1985
-1.224
(-)
17.4906-1160
18-4459-5178
18
2
0:00
0.5223
-0.2762
-1.703
( )
02,3993-1571
07-3005-SI71
19
16
0:00
0.8181
0.01963
0.121
01-9247-6416
11-1807-9439
20
16
0:00
0.7917
-0.006795
-0.04189
03-1157-3718
08-0843-9307
21
2022 Jan
5
0:00
0.8181
0,01963
0.121
13-9533-1111
05-1157-2603
12065 Lebanon Rd January 2022
� Mt. Juliet, TN 37122
acearralyica! Reference Toxicant Test
Nt?:'n 4;.;:i:sa:rv:vr [phone]
[phone] Fax
001-600-314-4 CETISTm v1.9.4.4 Analyst: g%`

Report Date: 14 Jan-22 09:23 (1 of 1)
CET1S QC Plot
Control Chart for January 2022 Chronic Minnows Reference Toxicant
Pace National
Test Type: Growth-5urvivai (7d) Organism: Pimephales promelas (Fathead Minn Material: Potassium chloride
P
Protocol: EpAl821lR-02-013 (2002)
Endpoint'. Mean Dry Biomass -mg Source: Reference Toxicant -REF
_
Control Chart for January 2022 Chronic Minnows Reference Toxicant
2s
-is
teen
-is
-2s
0 r �
N
O a
� Po
�•, o o v
� -
-
5681
0. 56a1
Count:
20
-Is Warning Limit: 0.4675
-2s Action Limit: 0.3669
Wean:
0,1006
CV: 17.70°/v +is Warning Limit: 0.6687 +2s Action Limit' 0.7693
Sigma:
Quality Control Data
QC Data
Delta
Sigma Action
Test iD
Analysis 1D
Point Year Month Day
Time
,
_Warning
15-8332-1340
05-6240-5700
1 2020 Dec 1
0:00
0.6478
0.07969
0.7921
054656-8971
1 3-2502-0305
2 15
0:00
0.5913
0.02325
0.2311
+ +
() ()
17-6414-7461
11-3112-5982
3 2021 Jan 5
0:00
0.7843
0,2162
2.149
�k)
01 8525-3287
j 1-2080-8220
4 26
0:00
4.69
0.1219
1.212
03-3817-1494
18-9606-2249
5 Feb 2
O:DO
0.4729
-0.09521
-0.9464
16-0978-6305
12-7053-1009
6 Mar 2
0:00
0.5696
0.001527
0.01518
45,4115_g214
04-0735-0967
7 Apr 6
0:00
0.5498
-0.01833
-0.1822
19-9828-92D7
i 3-0547-3591
8 MU 4
0:00
0.4959
-0.07223
-0.718
9
8
0:00
0.6152
0.04706
0.4678
174830-8595
13-8949-2788
10 Jun
8
0:00
0.6958
0.1277
1.27 H
09-9926-1346
07-4529-6124
11
29
0:00
0,7561
0.188
1.869 ()
08-0569-6381
00-0062-6277
12
Jul 13
0:00
0.5088
-0.05935
-0.69
09-8608-2358
08-4017-8716
13
10
0:00
D.5416
-0.08257
-0.8631
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09-6399-1286
14 Aug
14
UO
0.4855
-fl.08257
0.8208
00-7588-1410
06-9837-4819
15 Sep
7
0:00
0.4354
-0.1327
-1.319 (')
12-3809-4766
01-1962-5400
16 Oct
2
0:00
0.509d
-0.05$67
-0.5832
16-7844-7025
08-7128-8630
17 Nov
3
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0.4506
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12-5475-0605
18
7
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0.66
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10-9172-6309
02-9168-1813
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7
0:00
0.4894
-0.07874
-0.7827
01-3629-9711
01-2129-5494
20
4
0:00
0,4525
-0.1156
-1.15 (-)
04-6748-8542
04-6572-2587
21 2022 Jan
11065 Lebanon Rd
January 2022
laceAnalytical
Mt. iuliet, TN 37122
Reference Toxicant Test
Mvdm :q zjrmw "ot
(615)
773-2003
[phone] Fax
CETI.S7M �1.9.4.4
Analyst: Qk
001-600-314-4
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