NC0066516_Application_20190221

10P-V
f=JQJ/-\(-\/A-R!NA
1 )U 01 1 f lrobf t( I
February 21, 2019
Ms. Julie Grzyb
Water Quality Permitting Section — NPDES
Division of Water Resources
North Carolina Department of Environmental Quality
1617 Mail Service Center
Raleigh, NC, 27699-1617 RECEFVEWDFNR/DWR
FEB 2 e 2019
Re: NPDES Renewal Application Package Update
NPDES Permit Number NCO066516 Water ng Section
Permitting
Terrible Creek Wastewater Treatment Plant ing Se
Town of Fuquay-Varina
Dear Ms. Grzyb:
The Town of Fuquay-Varina Public Utilities Department is permitted to discharge 6.0 million gallons per
day (mgd) of treated effluent from the Terrible Creek Wastewater Treatment Plant (WWTP) to Terrible
Creek under North Carolina NPDES Permit NC0066516. The application package for the NPDES permit
renewal was submitted to the Division of Water Resources (DWR) on October 26, 2018. The renewal
application did not include the four required Second Species Toxicity tests. In the October 2018
correspondence, the Town committed to completing the required testing by the NPDES permit expiration
date of April 30, 2019. Attached to this correspondence are the following revisions to our NPDES
renewal application package:
• Revised EPA Form 2A Part E Toxicity Testing Data.
• Attachment (Tab F of October 2018 NPDES renewal application package):
Second Species Toxicity Tests dated October 2018, November 2018, December 2018, and
January 2019.
We appreciate the time and consideration of DWR for this Terrible Creek WWTP NPDES permit
renewal. If you have any questions regarding any of the NPDES permit renewal application materials,
please contact me at [phone] or [e-mail].
Sincerely,
Chris Grimes
Public Utilities Operation Manager
Town of Fuquay-Varina

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Terrible Creek WWTP, NCO066516
Renewal
Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd; 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E, you need not submit it again. Rather, provide the information
requested in question EA for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E.I. Required Tests. Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
® chronic ❑ acute
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 001 Test number: 002 Test number: 003
a. Test information.
Pimephales Promelas
Pimephales Promelas
Pimephales Promelas
Test Species & test method number
EPA1000.0
EPA1000.0
EPA1000.0
Age at initiation of test
< 48 hours
< 48 hours
< 48 hours
Outfall number
001
001
001
Dates sample collected
10/22/18, 10/24/18, 10/25/18
11/12/18, 11/14/18, 11/15/18
12/3/18, 12/5/18, 12/6/18
Date test started
10/23/2018
11/13/2018
12/4/2018
Duration
7 days
7 days
7 days
b. Give toxicity test methods followed.
Short -Term Methods for Estimating the
Short -Term Methods for Estimating the
Short -Term Methods for Estimating the
Manual title
Chronic Toxicity of Effluents and Recieving
Chronic Toxicity of Effluents and Recieving
Chronic Toxicity of Effluents and Recieving
Water to Freshwater Organisms
Water to Freshwater Organisms
Water to Freshwater Organisms
Edition number and year of publication
4th Edition, 2002
4th Edition, 2002
4th Edition, 2002
Page number(s)
pp. 53-111
pp. 53-111
pp. 53-111
c. Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used.
24-Hour composite
X
X
X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
X
X
X
After dechlorination
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 15 of 24

FACILITY NAME AND PERMIT NUMBER:
Terrible Creek WWTP, NCO066516
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Test number: 001 Test number: 002 Test number: 003
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected:
Final Effluent
Final Effluent
Final Effluent
f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
X
X
X
Acute toxicity
g. Provide the type of test performed.
Static
Static -renewal
X
X
X
Flow -through
h. Source of dilution water. If laboratory water, specify type; if receiving water, specify source.
Laboratory water
Soft Synthetic Freshwater
Soft Synthetic Freshwater
Soft Synthetic Freshwater
Receiving water
i. Type of dilution water. If salt water, specify "natural' or type of artificial sea salts or brine used.
Fresh water
Soft Synthetic Freshwater
Soft Synthetic Freshwater
Soft Synthetic Freshwater
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
0, 22.5, 45, 75, 90, 100%
0, 22.5, 45, 75, 90, 100%
0, 22.5, 45, 75, 90, 100%
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH
Yes
Yes
Yes
Salinity
N/A
N/A
N/A
Temperature
Yes
Yes
Yes
Ammonia
N/A
N/A
N/A
Dissolved oxygen
Yes
Yes
Yes
I. Test Results.
Acute: N/A
Percent survival in 100%
effluent
%
%
%
LC5o
95% C.I.
%
%
%
Control percent survival
%
%
%
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 16 of 24

FACILITY NAME AND PERMIT NUMBER:
Terrible Creek WWTP, NCO066516
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Chronic:
NOEC
100 %
100 %
100 %
IC25
N/A
N/A
N/A
Control percent survival
100 %
100 %
100 %
Other (describe)
m. Quality Control/Quality Assurance.
Is reference toxicant data available?
Yes
Yes
Yes
Was reference toxicant test within
acceptable bounds?
Yes
Yes
Yes
What date was reference toxicant test
run (MM/DD/YYYY)?
10/23/2018
11/13/2018
12/4/2018
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes, describe:
EA. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: N/A/ / (MM/DD/YYYY)
Summary of results: (see instructions)
Required NPDES toxicity monitoring is submitted quarterly to NCDWR
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 17 of 24

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Terrible Creek WWTP, NCO066516
Renewal
Neuse
SUPPLEMENTAL APPLICATION INFORMATION
PART E. TOXICITY TESTING DATA (continued)
POTWs meeting one or more of the following criteria must provide the results of whole effluent toxicity tests for acute or chronic toxicity for each of the
facility's discharge points: 1) POTWs with a design flow rate greater than or equal to 1.0 mgd; 2) POTWs with a pretreatment program (or those that are
required to have one under 40 CFR Part 403); or 3) POTWs required by the permitting authority to submit data for these parameters.
• At a minimum, these results must include quarterly testing for a 12-month period within the past 1 year using multiple species (minimum of two
species), or the results from four tests performed at least annually in the four and one-half years prior to the application, provided the results
show no appreciable toxicity, and testing for acute and/or chronic toxicity, depending on the range of receiving water dilution. Do not include
information on combined sewer overflows in this section. All information reported must be based on data collected through analysis conducted
using 40 CFR Part 136 methods. In addition, this data must comply with QA/QC requirements of 40 CFR Part 136 and other appropriate QA/QC
requirements for standard methods for analytes not addressed by 40 CFR Part 136.
• In addition, submit the results of any other whole effluent toxicity tests from the past four and one-half years. If a whole effluent toxicity test
conducted during the past four and one-half years revealed toxicity, provide any information on the cause of the toxicity or any results of a
toxicity reduction evaluation, if one was conducted.
• If you have already submitted any of the information requested in Part E, you need not submit it again. Rather, provide the information
requested in question EA for previously submitted information. If EPA methods were not used, report the reasons for using alternate methods.
If test summaries are available that contain all of the information requested below, they may be submitted in place of Part E.
If no biomonitoring data is required, do not complete Part E. Refer to the Application Overview for directions on which other sections of the form to
complete.
E. 1. Required Tests. Indicate the number of whole effluent toxicity tests conducted in the past four and one-half years.
® chronic ❑ acute
E.2. Individual Test Data. Complete the following chart for each whole effluent toxicity test conducted in the last four and one-half years. Allow one
column per test (where each species constitutes a test). Copy this page if more than three tests are being reported.
Test number: 004 Test number: Test number:
a. Test information.
Test Species & test method number
Pimephales Promelas
EPA1000.0
Age at initiation of test
< 48 hours
Outfall number
001
Dates sample collected
1/7/18, 1/9/18, 1/10/18
Date test started
1 /8/2019
Duration
7 days
b. Give toxicity test methods followed.
Short -Term Methods for Estimating the
Manual title
Chronic Toxicity of Effluents and Recieving
Water to Freshwater Organisms
Edition number and year of publication
4th Edition, 2002
Page number(s)
pp. 53-111
c. Give the sample collection method(s) used. For multiple grab samples, indicate the number of grab samples used.
24-Hour composite
X
Grab
d. Indicate where the sample was taken in relation to disinfection. (Check all that apply for each.
Before disinfection
After disinfection
X
After dechlorination
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 18 of 24

FACILITY NAME AND PERMIT NUMBER:
Terrible Creek WWTP, NCO066516
PERMIT ACTION REQUESTED:
Renewal
RIVER BASIN:
Neuse
Test number: 004 Test number: Test number:
e. Describe the point in the treatment process at which the sample was collected.
Sample was collected:
Final Effluent
f. For each test, include whether the test was intended to assess chronic toxicity, acute toxicity, or both
Chronic toxicity
X
Acute toxicity
g. Provide the type of test performed.
Static
Static -renewal
X
Flow -through
h. Source of dilution water. If laboratory water, specify type; if receiving water, specify source.
Laboratory water
Soft Synthetic Freshwater
Receiving water
i. Type of dilution water. If salt water, specify "natural' or type of artificial sea salts or brine used.
Fresh water
Soft Synthetic Freshwater
Salt water
j. Give the percentage effluent used for all concentrations in the test series.
0, 22.5, 45, 75, 90, 100%
k. Parameters measured during the test. (State whether parameter meets test method specifications)
pH
Yes
Salinity
N/A
Temperature
Yes
Ammonia
N/A
Dissolved oxygen
Yes
I. Test Results.
Acute: N/A
Percent survival in 100%
effluent
%
%
%
LC5o
95% C.I.
%
%
%
Control percent survival
%
%
%
Other (describe)
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 19 of 24

FACILITY NAME AND PERMIT NUMBER:
PERMIT ACTION REQUESTED:
RIVER BASIN:
Terrible Creek WWTP, NCO066516
Renewal
Neuse
Chronic:
NOEC
100 %
%
%
IC25
N/A
%
%
Control percent survival
95 %
%
%
Other (describe)
T_
m. Quality Control/Quality Assurance.
Is reference toxicant data available?
Yes
Was reference toxicant test within
Yes
acceptable bounds?
What date was reference toxicant test
1/8/2019
run (MM/DD/YYYY)?
Other (describe)
E.3. Toxicity Reduction Evaluation. Is the treatment works involved in a Toxicity Reduction Evaluation?
❑ Yes ® No If yes, describe:
EA. Summary of Submitted Biomonitoring Test Information. If you have submitted biomonitoring test information, or information regarding the
cause of toxicity, within the past four and one-half years, provide the dates the information was submitted to the permitting authority and a summary
of the results.
Date submitted: N/A/ / (MM/DD/YYYY)
Summary of results: (see instructions)
Required NPDES toxicity monitoring is submitted quarterly to NCDWR
END OF PART E.
REFER TO THE APPLICATION OVERVIEW (PAGE 1) TO DETERMINE WHICH OTHER PARTS
OF FORM 2A YOU MUST COMPLETE.
EPA Form 3510-2A (Rev. 1-99). Replaces EPA forms 7550-6 & 7550-22. Page 20 of 24

TAB F: Second Series
Toxicity Testing Results

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:1.1/1/2018
Facility: Fuquay Varina NPDES # NC00 66516 Pipe #: County: Wake
Signature of Laboratory Supervisor
Test Initiation Date[Time
% Eff, Repl.
Control Surviving #
Original #
Wt/original (mg)
. 22.5 Surviving #
Original #
Wt/original (mg)
F_75­1 Surviving #
Original #
Wt/original (mg)
75 Surviving #
Original #
'' Wtloriginal (mg)
F-9-F-1 Surviving #
Original #
Wt/original (mg)
100 Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
ip. at Receipt CC)
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621141ail Service Center
Raleigh, N.C. 27699-1621
FVTC 3:55 PM
1 2 3 4
Avg Wt/Surv. Control 0.924
10
10
10
10
10
10
10
10
1.016
0.890
0.967
0,823
10 _
10
10
10
10
110
10
10
0.885
0.793
0.850
0.758
10
10
1 10
10
10
10
10
10
0.780
0.877
0.847
0.710
10
10
10
10
10
10
10
10
0.680
0.961
0.822
0.807
10
10
10
10
10
10
10
10
0.716
0.998
0.794
0.698
10
10
10
10
10
10
10
10
0.845
0.834
0.905
0.762
Day
% Survival 100.0
Avg Wt (mg)0.924
% Survival 100.0
Avg Wt (mg) 0.822
% Survival 100.0
Avg Wt (mg) 0.8 44
%Survival 100.0
Avg Wt (mg) 0,815
% Survival 100.0
Avg Wt (mg) 0.802
% Survival 100.0
Avg Wt (mg) 0.837
0 1 2 3 4 5 6
Test Organisms
r Cultured In -House
I_ Outside Supplier
Hatch Date: 10/22/18
Hatch Time: 3:00 pm CT
8,02 / 7.82
8.17 17.99
8.08 / 7,90
8.15 / 8.14
8.27 1 8.04
8.26 / 7.86
8.22 / 7.86
7.84 1 6.68
7.53 / 7.40
1
7.90 / 6.80
1
7.45 / 7.72
7.95 / 7.46
1
7.68 / 6.89
1
7.70 / 6.73
24.4 / 24.0
24.9 / 24.6
25.8 / 24.8
24.3 1 24.7
24.2 / 24.8
24.4 / 24.2
24.1 / 25.0
0 1 2 3 4 5 6
7.18 / 7.53
7.19 / 7.73
7.33 / 7.66
7.23 ! 8.04
7.81 / 7.82
7.83 / 7.63
7.50 / 7.60
8.28 1 6.88
7.85 / 7.45
1
7.79 / 6.88
1
7.91 17.60
1
7.95 / 7.24
1
7,59 / 6.85
1
7.74 1 6,67
24.5 ! 24.6
25.0 / 25,0
25.5 1 24.8
25.1 / 24.3
24,5 / 24.4
24.9 / 24.3
25.3 / 24.7
1 2 3
10/22/2018
10/24/2018
10/26/2018
24.0
24.0
24.0
58
58
60
35
41
39
476
492
500
<0.1
<0.1
<0.1
1.2
1.1
1.3
Dilution H2O Batch # 1326 1327 1328
Hardness (mg/L) 44 42 48
Alkalinity (mg/L) 53 54 52
Conductivity (umhos/cm) 201 206 224
Survival
Growth Overall Result
Normal
F) ChV >100
Horn. Var.
FT
FI
NOEC
100
100
LOEC
>i00
>100
ChV
>100
>100
Method
Steel's
Dunnett's
Stats
Survival
Growth
Cone.
Critical Calculated
Critical Calculated
22.5
10 18
2.41 1.5785
45
10 18
2.41 1.8657
75
10 18
1 2.41 1.6786
90
10 18
2.41 1.8866
100
10 18
2.41 1.3475

Client: FVTC
NPDES #: 0066516>
Initiated by:
Date/Time Fed: 12/4/18
Date/Time Born: 12/3/18 3:00 Pry cr
Organism Source: Aquatox, Inc.
Transferred by:
Meritech, Inc. (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber. 10 Start Date: 12/4/18 Time:
Test Vessel Size: 400 ml End Date: 12/11/18 Time:
Test Solution Volume: 250 ml
Temp. of Stock: '2 V "K °C
# of Reps: 4
Test Organism: Pimephales promelas
Date
Time Initials
Day 1
12/5
Day
12/6
�.�{1,0,
Day 3
12/7
Day
12/8
07- �� G✓�`7n
Day 5
12/9J1
r"—
t
Day 6 12 10 Sri- 04, 2 /t'lt� G✓
Randomization: Yes No
incubator #: I_
Analyst(s): MR; CD, LV, SB
Fed by:
Date
Time 1 Initials
Time 2
Initials
Day 0
12/4
- 30n►� �-
- Q
f.�
Day 1
12/5
.11-3,n
0,L�
Day 2
12/ 6
10-'0qX q
Day 3
12/7
77. 3YAj wrn
L,,-)
Day-4
12/8
Day 6
12/ 10
/ a 3�n,
Test Termination Data:
Initials/Signature: [il
�v
End Date: 12/11/18

Client: FVTC
Ery.VIR¢NMENTAL LABORATOKIRS
A Ohf1s/on c+i VV.#fee Techinblagy, and Inc.
Chemical and Physical Determinations
NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Dav
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwater
Batch # :
/ %337
2
/ / 22 `l
?
l3KO
i3Yf -
--�
pH: Initial
rr
�� ,
U�
ra
F�51V
Final
C
'7L
"7• .
D.O.: Initial
'
`7 (�
7,
7
Final
- -27
7•
�9
Temp.: Initial
��
�(c
�i .
0
1�t'(
Final
o� �.
��c (�
�� on-
��{� ;
�,2
H _
Conductivity: Initial
. '
a5- Sr
Final
1' O,
a 3
Residual Chlorine:
<rn
Cc-,
CC,
zc)
Hardness:
L4a -
y q
Alkalinity:
cj�•
-
�Sri
Dav
Concentration: 22.50%
0-1
1-2
2-3
3-4
1 4-5
5-6 1
6-7
Remarks
pH: Initial
'7
q-3
7 '7 3
Final
7 62,
D.O.: Initial
2T3
'7-
Final
, 0
v
?"- t:7-
(O,S�,�
,
Temp.: Initial
Final
2S:Lfc
W ,
Conductivity: Initial
2,�-Z
f
a 7
(-
Final
02 `�
SD &
3 .
Residual Chlorine:
CO) (
Cw 1
<4
Day
Concentration: 45.0%
0-1
1-2
2-3
3-4 1
4-5
5-6
6-7
Remarks
PH: Initial
i �7
7�
S %
`�
y
Final
(p
7
D.O. Initial
i� q6
$ t O G
t
Final
'72
(Or `
-),4 q
Temp.: Initial
�J. U
k,.
d-
�� �P
q ,CJ
Final
k ,1
�u
a , '
��,
`� K S
9. q,s
Conductivity: Initial
o
-32
1 %
Final
?,
-3`A (
Residual Chlorine:
�Cu�
�U
��

Client: FVTC
ENVIRONMEN'T.AL }.f�6 cmArT R,65
.4 n%v:asan m/:W f.•r 7�!c,Yi.u/bp.Yi.n � Cnn rra/L., ,i.c.
Chemical and Physical Determinations
NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Dav
Page 2 of 2
Concentration: 75%
0-1
12
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Final
5
55
7. a9
•-7, q 3'
5
D,O.: Initial
Final
OA 1
—7 oZ
%i
�MS
7,10
U, (OS
Temp.: Initial
�iL
aS - 5
Final
2
Li�Q
Conductivity: Initial
Final
, j 7,
-
E 1
D
(�
�YD6
Residual Chlorine:
4cx-t
_
vj
��n �v
�u�)
t.)I I
Day
Concentration: 90%P07
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH Initial�
%%�
�.�(J
y
Final
u
��
71-.110
D.O.: Initial
Itd
3 • a
3
- j (U
%- &77
1 pgj
Final
DYS
Temp.: Initial
Final
��•
���
�S.''���
�`I
�S�-�
s
Conductivity: InitialLI
41
Final
117cg
`1
Residual Chlorine:
LLAti
C,
a"
Ccn
Zrii l
ecn l
e-a•:!
Da
Concentration: 100%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
`7,
7,va
' l
(i2cClS
�--;n
(�,
Final
%
r7-
D.O.: Initial
_
3`?<�i
�' �- -fs
Final
r 3
Temp.: Initial
a5 l
Cj •
`Z �(�
V-76
�CI -
Final
,S•
Conductivity: Initial
-7
Final
Residual Chlorine:
Lo,l
zfA(
Z-,m
I LcA
ZcAl
e) l
��
Hardness:
o
Alkalinity:
r
,

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC HPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11 /18
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
o --- --
Rep B 0
Rep C 0y IG I -
Rep D 0 --—
centration 22.50%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 a,
Rep B 0_
Rep C 0 �' d
Rep 0 0_ U 0
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B 0
Rep C 0
0
Rep D 0

MERITECH; INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 12/4/18
End Date: 12/11/18
Concentration 75%
Tuesday
Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
RepA_
0
Rep B
0
V
�
Rep C
0
Rep D
0
o
v
- oncentration
90%
Tuesday
Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
% '�
/
V
Rep B
0
Rep C 0_��
_
--�—
Rep D 0
/
Concentration 100%
Tuesday Wednesday Thursday
Friday
Saturday
Sunday Monday
Tuesday
Rep A 0
' _
/1
P
Rep B 0
—f_
---
Rep C 0
7
Rep D 0
_

ENVIRONMJE [NTAL LAB.ORA [F=S:
;y:..V :4 oryTsio rt cf Wate�Tec`(inoloyy and �ont[ols, (nc_
FISH LARVAL SURVIVAL/GROWTH DATA -SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC
NPDES #: 0066516
Date of Test: 1214/18
Initial weights taken on: by: tr-✓
Final weights taken on: by: (�
Page: 1 of 2
Outfall:
organism: Pimephales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT, (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
A
44
B�,
CCU rIn
n
C
�
0?7
Ko t
l0
qq
t
C
o
T00.
5,q
�
o
L_)t5W
/,(YD
A
931, ;20
3/c
67H
L/
•
to
22.50%
B
�' ,
Q�
o
���
5,
5�
0
,5.
A
C
5, 6�/
a "5ql
Zan
45.0%
B
4_
C
D
L�L-/, ­70
I g33:335
-3
1 to
G,S�_
,c

�NV.IRQNMENT.4L IABOFLA70R[ =S.
A AlVis%n 6f Water 'Toe inoloyy 017d conpals, /nc_
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC
NPDES #:.0066516
Date of Test: 1214/18
Initial weights taken on: by: - CV
Final weights taken on: by:—L�2
Page: 2 of, 2
Outfall:
organism: Pimephalespromelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
#ORG.
MEAN
WTJORG. (mg)
SURV %
o
75/o
s
tC�,
� Q/l 9
/'�j
of
c.(��
ter.
C
l
ELI
90%
A
QCS✓,
l
8
ERa(ga
o.Qd
0
a5,6?
6,
q6
1,06
D
.)30 �),J
! Lam
+
o- 0
o
100 �o
A
I7, 9
31-76'
k.)
o.(-,()()
B
W9. 5
N •7�
�
0
0
57LI
0, 57

MERITECH, INC. MerlEechSample lD
Bloassav_Sample Chain of.Custody_-
642 Tamco Rd, Reidsville NC 27320
_- Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reedOmeritech-labs.com Web Site: www.meritech-labs.com
�(' CLIENT INFORMATION
Client: 1LXZV- b� FU4U4-! PO#:
Contact Person: NPDES#: NCt3U(o4J�
Address: .S4(t2 (4,!t W QA- Phone:
City: � j!t. ;Pipe #:
County Zip: Z.75--Zic
SAMPLE INFORMATION
Sample Site: I eff4, C644,4`'r 1�
Spmple Type: ❑ Grab Composite # of containers: -
Sampling Time: Start Date: Z-3-1 `d StartTime; `cl J AP 'PM
End Date: I2 - (-(-1 Y End Time: A0 PM
*** Triple rinse sample container with sample before filling. Completely till the sample .container with no air space.
Pack the sample cooler complette(ly in ice. The sample must be < 6.0°C upon receipt at the laboratory***
Collector's Name: Print: 6� +1.c,,� Signature: i
TOXICITY TEST INFORMATION
st Required: Chronic (7 days) Test Organism: [� Ceriodaphnia dubla (water Sea)
❑ Acute (24-48 hours) Pimephales promelas. (fathead minnow)
❑ Mysldopsis bahla (shrimp)
IWC: o %
Comments:
Test Concentrations (if multiple dilutions):
/i SNIPPING INFORMATION
Relinquished by [.,i! �(. S I� t yr .n Date: L-t•j-1 V
Received by: Date: �a ::w -1
Relinquished byia�k _ Date: j —0Z-
Received by: Date:
Relinquished by:
Received by:
Relinquished by.
Received by:
Date:
Date:
Date:
Date:
Sample Temperature CC):
Time: d% ! S ? AM PM
Time: ,' .
AM
PPM,
Time: L �^ r
AM <P5
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Time:
AM
PM
Method of Shipment: ❑ UPS ❑ Fed EC I Meritech Pick-up ❑ Delivered ❑ Other
•" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED'*
SAMPLE RECEIVING (Laboratory Use Only)
�,alinquished by:
Received by: Date: 1, Time:410 AM �M
Sample Temperatures CC): "3 / r ' I I Sample Condition:
_ INLIITC — 1 NPI t 111W O r1ionf nnnv

MERITECH, INC. .-MerltechSample ID#: ,'�)06 SA3
Bloassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed meritech4abs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: Bch o fv _ flhl ? , PO#:
Contact Person: f C64 ^.%San NPDES#: NC 00 46 51 C.
Address: Phone:
City: Pipe #:
County State: Zip:
SAMPLE INFORMATION
Sample Site: L!P Cree- t'1 �LOoen
Sample Type: ❑ Grab Composite # of containers:
Sampling Time: Start Date: Start Time: 07o0 AM PM•=
End Date: ` 2 -6-End Time: 0906• AM PM:"
*** Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt. at the laboratory***
Collector's Name: Print: )'ro a Signature;
TOXICITY TEST INFORMATION
est Required: Chronic (7 days) Test Organism: Cerlodaphnia dubia (water flea)
El Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
lwc. 90 % Test Concentrations (if multiple dilutions):
Comments:
` SHIPPING INFORMATION tc�
r Z' ��1
Time: ��
AM(!!2
Relinquished by: Irc� 1')<✓t
Date:
Date:
o
j
Time: �a r s
AM
M
Received b
Relinquished b ' G—
Date:
�a
time:
AM
P }
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date;
Time:
AM
PM
Date:
Time:
AM
PM
Received by:
Relinquished by:
Received by:
Date:
Date:
Sample Temperature eC):
Time: AM PM
Time: AM PM
Method of Shipment:❑ UPS ❑ Fed EX Meritech Pick-up ❑Delivered ❑ Other
Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, No SIGNATURE REQUIRED
,.elinquished by: "I / {1r fi
Received by: Date: t Time: AMM
Sample Temperatures CC): l l 1 Sample Condition:
UU411TP 1 ahnrafnry cnnu YELLOW = Client coov

MERITECH, INC. MeritechSample ID
. _ . Bioassay Sample Chain of Custody:_ _
" 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: [e-mail]. Web Site: www.M6ritech,labs.com
CLIENT INFORMATION
Client:i/� FiJp,v - �i r�✓ty PO#:
NPDES#: NC (7Q(6St.6.
Contact Person: "in 4 t�nr,041
Address: Phone: ,t
City: Pipe
County: State: Zip:
��- SAMPLE INFORMATION
Sample Site: ►Pll: btr L,(te jj E -60y-
L
I
Sample Type: ❑ Grab Composite # of containers:
Sampling Time: Start Date: >>g Start Time:—) -00 (AMM)
0M
End Date: 12- - %' 1 b End Time: 11 ' (%O - PM
Triple rinse sample container with sample before tilling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 60C upon receipt at the laboratory'*
Collectoes Name: Print: _f J � VI e, Signature: -
TOXICITY TEST INFORMATION
TAst Required: 0 Chronic (7 days) Test Organism: Cerlodaphnla dubla (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsls bahla (shrimp)
IWC: % Test Concentrations (if multiple dilutions):
Comments:
Relinquished by: r-
Received by:
Relinquished by: /G�.
SHIPPING INFORMATION a
Date; IL- % ��lo
Date: _�� / 1$_
�- Date: �oZ / 7 �% 9
Time: _ �a �d
Time: ���a�����
Time: / 1 0
—
AM
AM PM
AM EM
Received by:
Dater
Time:
AM PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Relinquished by:
Date:
Time:
AM
PM
Received by:
Date:
Time:
AM
PM
Sample Temperature (°C):
Method of Shipment:❑ UPS ❑ Fed EX [A Meritech Pick-up ❑Delivered ❑ Other
'• Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED ••
y-ilinquished by: t rr� ``
Date: z � Time: �y AM PAM
Received by: -
Sample Temperatures (°C): I_I v I Sample Condition:t!C
WHITE = Laboratory copy YELLOW = Client copy

Title: FVTC
F, FVTCGrowth Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
-----------------------------------------------------------------------------
D = 0.0493
W = 0.9755
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: FVTC
F-"- . FVTCGrowth Transform; NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 6.2510 (p-value = 0.2826)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
----------------------------------------------------------------------------
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)
I

Title: FVTC
F, FVTCGrowth Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE
DV
SS
--______
MS F
- ---------------
Between
- --__-_
5
-
0.0213
0.0043 1.5552
Within (Error)
18
0.0493
0.0027
-------------------
Total
23
0.0706
----------------------------------------------------------(p-value
- 0.2229)
Critical F =
4.2479 (alpha =
0.01, df = 5,18)
=
2.7729 (alpha =
0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title:
F"I"
FVTC
FVTCGrowth
Transform: NO
TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
-------------------------------------------------------
TRANSFORMED
MEAN CALCULATED
IN SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
T STAT 0.05
--------------------------
1
Control
-
0.6135
0.6135
2
22.5
0.5760
0.5760
1.0135
3
45
0.6395
0.6395
-0.7027
4
75
0.5923
0.5923
0.5743
5
90
0.6625
0.6625
-1.3243
6
100
0.5938
0.5938
0.5338
Dunnett
--
critical value= 2.4100 (1 Tailed,
alpha---0.05, df
= 5,18)
Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
____________________________NUM
-OF---
MIN SIG DIFF
o OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS ,
(IN ORIG. UNITS)
CONTROL
FROM CONTROL
Control
4
22.5
4
0.0892
14.5
0.0375
3
45
4
0.0892
14.5
-0.0260
4
75
4
0.0892
14.5
0.0212
5
90
4
0.0892
14.5
-0.0490
6
100
4
0.0892
14.5
0.0198

Title: FVTC
F-" FVTCSurvival Transform: NO TRANSFORMATION
Shapiro -- Wilk's Test for Normality
D = 0.0225
W = 0.6683
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation..
Warning -- The first three homogeneity tests are sensitive to non --normality
and should not be performed with this data as is.

Title: FVTC
W - FVTCSurvival Transform: NO TRANSFORMATION
Hartley's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.
----------------------------------------------------------------

Title: FVTC
74'-^ FVTCSurvival Transform:
NO TRANSFORMATION
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
__..___________---------------
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
VALUE
DF 0.05
1
Control
1.0000
2
22.5
1.0000
18.00
10.00
4.00
3
45
1.0000
18.00
10.00
4.00
4
75
0.9750
16.00
10.00
4.00
5
90
0.9750
16.00
10.00
4.00
6
100
0.9750
16.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values. are 1 tailed ( k = 5 )

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date:11/28/2018
Facility'. Fuquay Varina
] gboAtory: Meritech, Inch
of Operator.
V.
Signature of Laboratory
NPDES # NC00 66516 Pipe M County: Wake
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Test Initiation Date/Time 11/13/2018 4:25PM Avg Wt/Surv, Control 0.779
% Eff. Repl. 1 .2 3 4
Control Surviving #
Original #
Wt/original (mg)
_22.71 Surviving #
Original #
Wt/original (mg)
45 Surviving #
Original #
Wt/original (mg)
75 Surviving #
Original #
WUoriginal (mg)
90 Surviving #
Original #
Wtloriginal (mg)
100 Surviving #
Original #
Wtloriginal (mg)
iter Quality Data
Control
pH (SU) Init/Fin
DO (mgtL) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) lniUFin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
p. at Receipt (°C)
10
10
10
10
10
10
10
10
0.850
0.697
0.769
0.801
10
10
10
10
10
10
10
10
0.791
0.632
0.748
0.767
10
10
10
10
10
10
10
10
0.608
0.651
0.739
0.768
10
10
10
10
10
10
10
10
0.714
0.736
0.706
0.743
10
10
10
10
10
10
10
10
0.717
0.687
0.693
0.752
10
10
10
10
10
10
10
10
0.679
0.708
0.667
0.733
% Survival 100.0
Avg Wt (m
g)F 0.779
% Survival 100.0
Avg Wt (mg) 0.735
% Survival 100.0
Avg Wt (mg) 1 0.692
% Survival 100.0
Avg Wt (mg) 0.725
%Survival i00:0
Avg Wt (mg) 0.712
% Survival 100.0
Avg Wt (mg) 0.697
Day
n 1 2 3 4 5 6
Test Organisms
{M Cultured In -House
r_ Outside Supplier
Hatch Date: 11/12/18
Hatch Time: 3:00 pm CT
8.03 1 7.99
8.15 / 7.96
8.07 / 7.73
8.13 17.97
8.13 1 8.07
8.22 1 7.92
8.11 / 7.69
7.88 1 7.30
7.79 / 7.73
7.87 16.75
7.62 / 7.83
8.05 1 7.50
8.17 17 34
7.69 / 6.55
25.1 / 24.1
24.6 1 24.5
24.7 124.6
25.0 / 24.3
25.0 1 24.5
25.4 1 24.5
24.0 / 24.6
0 1 2 3 4 5 6
6.95 1 7.65
7.12 1 7.70
7.17 17.63
7.37 / 8.04
7.92 18.07
8.05 / 7.78
7.50 17.71
8.18 1 7.44
8.04 1 7.18
8.22 1 7.00
8.19 17.85
8.12 1 7.47
8.03 i 7.20
7.92 / 6.45
25.7 / 24.6
25.5 1 24.7
24.8 1 24.2
24.8 1 24.7
25.9 1 25.2
25.3 1 24.7
24.8 1 24.9
1 2 3
11 /12/2018
11 /1412018
11/15/2018
24.0
24.0
24.0
54
50
48
51
56
56
479
384
399
<0.1
<0.1
<0.1
1.6
1A
1.6
Dilution H2O Batch # 1332 1333 1334 1336 1336
Hardness (mg/L) 44 42 44 44 44
Alkalinity (mg/L) 54 53 52 55 48
Conductivity (umhoslcm) 209 185 200 195 187
Survival
Growth .
Normal;
F�
Hom. Var.
7
NOEC
100
100
LOEC
>100
>100
Chi/
>100
>100
Method
Steel's
Dunnett's
Overall Result
ChV F >100
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
22.5
10 18
2.41 1 A 986
45
10 18
2.41 2.3502
75
10 18
2.41 1.4597
90
10 18
2.41 1.7945
100
10 18
2.41 2.2096

Client: FVTC
NPDES A 0066516
Initiated by: w
Date/Time Fed: 1/8/19
Datelrime Born: 1/7/19 3,00 PM CT
Organism Source:. Aquatox, Inc.
Meritech, Inc. (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber 10
Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
Temp. of Stock:-q•') -°C
# of Reps: 4
Test Organism: Pimephales promelas
Start Date: 1/8/19
Time:
End Date: 1/15/19
Time:
Randomization:
Yes No
Incubator #: i
Analyst(s): MR, CD, LV, SB
Transferred by: Fed by:
Date Time initials Date Time 1 Initials Time 2 Initials
Day 1 1/9 % I 4j �w Day 0 1/8 .1 i�� 7r '112
Day 1 1/9 . 30 A2
Day 2 1/10 f
Day 3 1/11 t,)/(-L Day 2 _ i/10 fifl
Day 3
4 ]12
Day /) --
''11 n Day 4
1/13
Days r U
Day 1/14 -� Days 1/13 L�
'OA—
lac.};
Day 6 1/14 �- �,E d'1CSS
Test Termination Data:
Initials/Signature:
End Date:

TV
• ENVIRONIVIEIVTA I:ABORA DRIES r
•• - •o vfsfo»'c+f Water:?-ech o
.- ..: _ ..: - ..: r •> .. .. tocJX �hd. Controls, IrYc. ..
Chemical and Physical Determinations
Client: FVTC NPDES#: 0066516 Start Date: 1/8/19
End Date: 1/15119
Day
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwater
Batch 0 :
q 3
l
h
pH: Initial
U
�, a�,r)
Final
i
,l�(
�•
L
-71-71
D.O.: Initial
Pj,4
Final
rS
u-9
7i• 5�
677(2
Temp.: Initial
`
Final
Conductivity: Initial
Cj (
��
W
13
,3
1
x
Final
Residual Chlorine:
Hardness:
L4
Alkalinity:
c r
, -
s52
Day
Concentration: 22.50%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
PH: Initial
lU
, 03
I a o
Y, v
Final
D.O.: Initial
�
, G
n�i7
�Jj�'t5-
(p
1 %
0
Final
2
�
7Temp.:
17
Initial
02
Final
�f
S
r9` �- ��.
�% f
7 . t_l
'AfJ
112 1-1 . 3
Conductivity: Initial
2l
9Nk
q"4-p-
23( ,
Final
3 y
r}
Residual Chlorine:
rx
nZoi,<f-SQ
I
ZP„�,
Day
Concentration: 45.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
f
7, 20
1
Final
7^ a
7, K 3
q,, Q
%►
D.O.: Initial
Final
Id
7 "j(}
�• Q ,
Temp-: Initial
�, -
r
_
v2�
;,�� �`
kf
Finallj
Conductivity: Initial
5bY
379
-,
.
Final
5
3�
Residual Chlorine:alCam;
i
Cca (
ut
Ccn

7-2
' '': �+Y+%v/ /u.r�i/.W M�� rrrhisis%uq!'••un'�l '2:nri�iti�Ys�, /Nc.
Chemical and Physical Determinations
Page 2 of 2
Client: FVTC NPDES#: 0066516 Start Date: 1/8/19
End Date: 1115/19
Concentration: 75%
0-1
1-2
2-3
.3-4
4-5
5-6
6-7
Remarks
pH: Initial
W7
5,
1 '
71 71
T''
Final
j-',
D.O.: Initial
Final
�Ur
.,
%i 5—
2-�
Temp.: Initial
/J
Final.
Conductivity: Initial
cJ U
0
�b
Li 15-
I)
Final
G
? -i a'.
Residual Chlorine:
4(A
C�'►
C� (
Cam'
�GL
Day
Concentration: 90%
0-1
1-2
2-3
-3-4
4-5
5-6
6-7
Remarks
pH: Initial
L a
! �}
l-I
-/•'6
,7 -,)-a
Final
���
zGL�
`7 10
D.O.: Initial
Final
Z 3�5
(:
Temp.: Initial
, y . C
Final
e
�L tl1 %
?,X� ,
`,t . 7
Conductivity: Initial
3 ,
q.�
t0
Final
II ,,S
71-g
Residual Chlorine:
ZoL I
C�x �
---c-�-
C/t
Day
Concentration: 100%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
•�
!1.
-1?��
.- r
(15
Final
0
9.n
^ '1
7+ 2
c�
%�✓'�J
D.O.: Initial
.�
-S-z
Final'
3-
Temp.: Initial
rj�,
o 6 •
�`t, �
�
g
fa4,FinalConductivity:
Initial
1
q.
- - Final
7
Residual Chlorine:
j1—,,
Z-'A.
{
Hardness:
H Cc
Alkalinity:
7Q,70
• i

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 1/8/19
End Date: 1/15/19
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
v
�2
ti-
Rep B 0
Rep C 0
Rep D 0 -- �-
-ncentration 22.50%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 P')) �)
l/
Rep B 0 d 1 "
v 7���
Rep C 0 �Z_IL—
Rep D 0 v_ D
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 t_ U 0 .r) - _/ V
Rep B 0� �)
Rep C 0
12
Rep D 0

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066616 Start Date: 118/19
End Date: 1/16/19
Concentration 75%
Tuesday
Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
Rep B
0
n_
Rep C
0
—--
��
Rep D
0
(_7+
f"oncentration
90%
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A
0
_
D
Rep B
0
0
Rep C
0
^�
(J
0
Rep D
0
/v
Concentration 100%
Tuesday Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A 0 r
-__�
0
--—
Rep B 0
Rep C 0
Rep D 0

Ar
/ N: r:; Api3NSicaioF, LNa;erTe�linol5�'rjy.•�{2dConF�gls�n�
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC
NPDES #: 0066516
Date of Test: 1 /8/19
Initial weights taken on: 1,2-9- 7 r' by: (A/
Final weights taken on: 1-16—: by:
Page: 'I of 2
Outfall:
Organism: Plmephales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
A
R1
.F
B/
I
U ( IJ
c
�,Ll`�..
T
�.�97
F.jl f n
71 f2 F
C .
01=
0
A
'33 1,��'
`7r 7,
0,7
B
97,
—7,
�► /�
22.50%
�s`3
6r
1
, �
lv�
D
,�14
1
� r'
0
0165`
lC�
A
45.0%
B.
��
D

_,r ' - ' .. -'.'ENYt�Zi]NM.�MTAL'L•'AS:Of2A'i:OFEIES' .: .
Ec
._ '. �:'`�Ad?iYtsiorf.dF, Wat'e7eclinoloyy.und Conlrojs,"%nT:, , -
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 2 of 2
NPDES #: 0066516
Date of Test: 118119
Initial weights taken on: by:
Final weights taken on: by:—
Outfall:
Organism: Pimephates promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT.IORG. (mg)
SURV %
75�ia
A
3�.
cZ
, '
��
��� 7��C�109
s.
��
�a���
0t(
C
�7� V4
! ic7
ro
D
3�7 i�
��
G
10
90%
A
173
1
-7, 7 5
l0
6-7-75
166
B
�� d
95,5,5
o
O, �5'
10�
�e
� .7�f
ld
a, 67eci
Or-)
D
O,a
F7
' 100%
A
(?1-715(0
b-�25)14/
1
/0
01
< <
l v
017
D
39,
F3gH6
667
q
046:�Z
q0

MERITECH, INC. (Lab #027) Medtech Sample ID #:
Bloassay Sample Chain of Custody
' 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike reedCr7meritechlabs.com Web Site: www.meiitechiabs.com
CLIENT INFORMATION
C„y w ID
Client: � +rPO#: y`i
Contact Person: Sf ,ree NPDES##: NCd0&&6't,c,.
Address: �foT 147 1 ¢, Phone:
City: Fi-x-lof!n1 Pipe #:
County: ljcy%C-�-
State: Zip: Zz5'z 4
samplesite: Tcir16l(_ Crtc lv G T �
Sample Type: ❑ Grab 4 Composite
SAMPLE INFORMATION
# of containers: 7-
Sampling Time: Start Date: 1-7 -) `t Start Time: U`t=O-J AP PM
End Date: I -5r-- Iq End Time: 0i::0 A0 PM
*** Triple rinse sample container with sample before filling completely with NO AIR SPACE. Pack the sample cooler completely with ice. The
sample must be <00C upone receipt at Meritech`
Collector's Name: Printri 5tyw`^ Signature:
TOXICITY TEST INFORMATION
L; `A Required: ( Chronic (7 days) Test Organism: E Cerlodaphnia dubla (water flea)
—' ❑ Acute (24-48 hours) [P P#mephales promelas (fathead minnow)
❑ Mysidopsis bahla (mysid shrimp)
IWC: 91-) % ***Friday Samples for Chronic Fathead test must be collected after 9:00 a.m. on Friday*`*
Comments:
SHIPPING INFORMATION
Relinquished by: Date: Time: AM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: �& O C) AM P
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Relinquished by: Date: Time: AM PM
Received by: Date: Time: AM PM
Sample Temperature CC):
Method of Shipment:❑ UPS ❑ Fed EX P Meritech Pick-up ❑ Delivered [] Other
`* Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED `*
SAMPLE RECEIVING (Laboratory Use Only)
�;Naltnquished by: aa CO
Received by: Date: t Gl AM
Sample Temperatures CC): r 1 1 1 Sample Condition:
InrwsC - i ohnrz%+r,r%r rnnv YELLOW = Client copy

MERITECH, INC. (Lab #027) Meritech Sample ID #:
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
1 Toxicity Supervisor email: mike.reed(o)meritechiabs.com Web Site: www.meritechiabs.com
CLIENT INFORMATION
Client: TOw, oP Fuwq - POM
Contact Person: NPDES#: NCdUGf r1,4,
Address: S t-m 0 d fl i2 Z Phone:
city: { d4ul , ! Pipe #:
County: J(J4V-'- State: Nc- Zip: Z?tzc�
SAMPLE INFORMATION
Sample Site: Rt--,biY-- Ceiri✓`
Sample Type: ❑ Grab Composite # of containers: Z
Sampling Time: StartDate: _ I-4-t Y Start Time: 04; vJ AV PM
End Date: I -"10 -t 1 End Time: U '_ v.� A0 PM
*** Triple rinse sample container with sample before filling completely with NO AIR SPACE. Pack the sample cooler completely with ice. The
sample must be <0.6°C upone receipt at Meritech***
Collector's Name: Print: �S � Signature:
TOXICITY TEST INFORMATION
r- st Required: [ Chronic (7 days) Test Organism: [1 Ceriodaphnia dubia (water flea)
❑ Acute (24-48 hours) [1 Plmephales promelas (fathead minnow)
❑ Mysidopsis bahla (mysid shrimp)
IWC: 1t7 % ***Friday Samples for Chronic Fathead test must be collected after 9:00 a.m. on Friday'
Comments:
SHIPPING INFORMATION
Relinquished by: 6k r-5 E-( Date: to -[
Received by: Date: j 0
Relinquished by: Date: % /2 Q Z/ 9
Received by: Date:
Relinquished by:
Received by:
Relinquished by:
Received by:
Date:
Date:
Date:
Date:
Sample Temperature (°C):
Time:
Time:
Time:
Time:
Time:
Time:
Time:
Time:
//moo 0 PM
o AM PM
/.3 So AM PM
AM PM
AM PM
Method of Shipment: ❑ UPS ❑ Fed EX ❑ Meritech Pick-up ❑ Delivered ❑ Other
AM PM
AM PM
AM PM
I Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED **
.-inquished by: Z I
Received by: Date: v Time: AM >SU r
Sample Temperatures (*C): ! ! / Sample Condition: ' `?
1AlLIITC - 1 ok-r.t- rnnv Y1=1 1 nw = t'-linnf enhv

MERITECH, INC. (Lab #027)
Meritech Sample ID #:
k..
Bioassay Sample Chain of Custody
"
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
ter`
Toxicity Supervisor email: mike.reed(ameritechlabs.com Web Site: www.maritechiabs.com
CLIENT INFORMATION
Client:
Ip r ✓
PO#.
Contact Person:
Ah. ST''e
0 hsb+.
NPDES#: NC 00&6 ,P C�
Address:
aI..
Phone:
City:
uti -
V<i i h` k
Pipe #:y°
County:
W c A44
/ .
State: V C Zip:
75'.16
SAMPLE INFORMATION
Sample Site: �C w W TP
Sample Type: El Grab D omposite # of containdrs: 3
Sampling Time: • Start Date: 11 l 1 Start Time: O = �-� AM PM
End Date: ! ~��� 19 End Time: %O 45 AM PM
***Triple rinse sample container with sample before filling completely with NO AIRSPACE. Pack the sample cooler completely with ice. The
sample must be <0.6°C upone receipt at Meritech***
Collector's Name: Print: Signature: -�/e i7.IstS
�� TOXICITY TEST INFORMATION
st Required: [2 Chronic (7 days) Test Organism: [] C riodaphnla dubia (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (mysid shrimp)
IWC: E) % `**Friday Samples for Chronic Fathead test must be collected after 9:00 a.m. on Friday*`*
Comments:
SHIPPING INFORMATION G
Relinquished by: rK" Y)el Date; J /
Received by: Date:
Relinquished b Date:
Received by: Date:
Relinquished by:
Received by:
Relinquished by:
Received by:
Date:
Date:
Date:
Date:
Time: /3 020 AM P
Time: /S;t 0 AM P
Time: �-s oZ AM �M
Time: AM PM
Time: AM PM
Time: AM PM
Time: AM PM
Time: AM PM
Sample Temperature CC):
Method of Shipment: ❑ UPS ❑ Fed EX 12/Meritech Pick-up ❑ Delivered ❑ Other
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED
--„-Jinquished by:
Time:
Sample Temperatures VC):
Sample Condition:
IAIUI C - i ahnrn#^ry rnnv YFI 1 i M = rliPnt ennv

Title: fuquay
fuquay Transform: NO TRANSFORMATION
Shapiro - Wilkes Test for Normality
----------------------------------------------------------------------------
D = 0.1204
W = 0.9659
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: fuquay
fuquay Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 1.5589 (p-value = 0.9062)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
-----------------------------------------------------------------------------
Critical B = 15,0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Title: fuquay
fuquay Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE
DF
SS
MS F
------------------------------------------------------------------------
Between
5
0.0072
0.0014 0.2142
Within (Error)
18
0.1204
0.0067
Total
23
0.1276
--------------------------------------------------------------------------
(p-value = 0.9519)
Critical F =
4.2479 (alpha =
0.01, df = 5,18)
=
2.7729 (alpha =
0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title:
fuquay
File:
fuquay
Transform: NO
TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED
MEAN CALCULATED
IN SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
T STAT 0.05
------
-----
I
--------------------
control
-----------
0.6865
------------------
0.6865
2
22.5
0.7070
0.7070
-0.3544
3
45
0.6498
0.6498
0.6354
4
75
0.6820
0.6820
0.0778
5
90
0.6708
0.6708
0.2723
6
100
0.6840
0.6840
0.0432
----------------------------------------------------------------------------
Dunnett
critical value = 2.4100 (1 Tailed,
alpha = 0.05, df
= 5,18)
Title: fuquay
File: fuquay Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
------------------
7---------------------------------------------------------
NUM OF
MIN SIG DIFF
% OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
(IN ORIG. UNITS)
CONTROL
FROM CONTROL
control
4
22.5
4
0.1394
20.3
-0.0205
3"
45
4
0.1394
20.3
0.0367
4
75
4
0.1394
20.3
0.0045
5
90
4
0.1394
20.3
0.0157
6
----------------------------------------------------------------------------
100
4
0.1394
20.3
0.0025

Title: fuquay - survival
File: fuquaysury Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.0650
W = 0.,8435
Critical W = 0.8840 (alpha = 0.01 N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data FAIL normality test (alpha = 0.01). Try another transformation.
Warning - The first three homogeneity tests are sensitive to non -normality
and should not be performed with this data as is.

Title: fuquay - survival
File: fuquaysury Transform: NO TRANSFORMATION
H�.s-iey's Test for Homogeneity of Variance
Bartlett's Test for Homogeneity of Variance
These two tests can not be performed because at least one group has
zero variance.
Data FAIL to meet homogeneity of variance assumption.
Additional transformations are useless.

Title:
fuquay - survival
File:
fuquaysury
Transform:
NO
TRANSFORMATION
Steel's Many -One
Rank Test
- Ho:
Control<Treatment
----------------------------------------------------------------------------
MEAN IN
RANK
CRIT.
SIG
GROUP
IDENTIFICATION
ORIGINAL UNITS
SUM
-------
VALUE
------
DF 0.05
----- ---
-------------------------
1
control
-----------
0.9500
2
22.5
0.9500
18.00
10.00
4.00
3
45
0.9500
19.00
10.00
4.00
4
75
0.9750
20.00
10.00
4.00
5
90
1.0000
22.00
10.00
4.00
6
100
0.9750
20.00
10.00
4.00
----------------------------------------------------------------------------
Critical
values are 1 tailed ( k = 5 )

Effluent Toxicity Report Form -Chronic Fathead Minnow Mufti -Concentration Test Date:12/14/2018
Facility: Fuquay Varina
Signature of Operator in
Signature of Laboratory Supervisor
NPDES # NC00 66516
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Pipe #: County: Wake
Comments
Test Initiation Date/Time 12/4/2018 4:36 PM Avg WE/Surv. Control 0.614
6ff. Repl. 1 2 s 4
Control Surviving #
Original #
Wt/orlginal (mg)
22.5 Surviving #
Original #
Wt/original (mg)
F-4-5-1 Surviving #
Original #
Wt/original (mg)
75 surviving #
Original #
Wt/original (mg)
90 Surviving #
Original #
Wt/original (mg) -
Surviving #
Original #
Wt/original (mg)
Quality Data
Control
pH (SU) Init/Fin
DO (mglL) Init/Fin
Temp (C) InitlFin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Ternp (C) InitlFin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
p. at Receipt (°C)
10
10
1 10
10
10
10
1 10
10
0.628
0,652
1 0.644
0.530
10
10
10
10
10
10
10
10
0.675
0.554
0.499
0.576
10
10
10.
10
-10
10
10
10
0.591
0.706
0.699
0.563
10
10
9
10
10
10
10
10
0.613
0.594
0.611
0.551
10
10
10
9
10
10
10
10
0.687
0.620
0.695
0.648
10
10
10
9
10
10
10
10
0.600
0.581
0.620
0.574
% Survival 100.0
Avg Wt (mg) 0.614
% Survivall 100.0
Avg Wt (mg) 0.576
% Survival 100.0
Avg Wt (mg) 0.640
%° Survival 97.5
Avg Wt (mg) 0.592
% Survival 97.5
Avg Wt (mg) 0.653
% Survival 97.5
Avg Wt (mg) 0.594
Day
n 1 2 3 4 5 6
Test Organisms
r': Cultured In -House
rv'� Outside Supplier
Hatch Date: 12/3/18
Hatch Time: 3:00 pm CT
8.09 / 7.92
8.20 / 7.93
8.21 / 8.16
8.27 / 7.83
8,21 1 '7.72
8.20 1 7.87
8.07 / 7.81
7.71 1 7.09
7.66 17.27
7.69 / 6.96
7.68 / 6.88
7.68 17.47
7.85 1 6,48
7.62 1 6.99
24.8 124.5
24.8 124.6
24.4 / 25.2
24.7 1 24.5.
25.4 / 24.9
25.2 / 24.9
24.9 / 24.1
n 1 7 3 4 5 6
7.19 / 7.80
7.50 1 7.86
7.00 1#7.43.16
/ 7.36
6.95 1 7.16
8.22 / 7.02
6.74 / 7.128.35
/ 7.04
7.59 / 7.19
8.39 /.72
/ 7.09
8.26 / 7.42
7.85 / 6.41
8.32 / 6.8325.1
/ 25.6
25.2 / 25.4
24.9 15.3
/ 24.3
25.0 / 24.6
24.7 ! 25.0
25.7 / 25.3
1 2 3
12/3/2018
12/5/2018
1216/2018
24.0
24.0
24.0
48
58
60
56
27
14
422
469
478
<0.1
<0.1
<0.1
1.3
1.8
1.8
Dilution H2O Batch # 1337 1338 1339 1340 1341
Hardness (mg/L) 42 44 44 42 44
Alkalinity (mglL) 55 57 58 59 51
Conductivity (umhos/cm) 199 219 233 213 209
Survival
Growth Overall Result
Normal
171
i✓j ChV >100
Hom. Var.
a
F UI
NOEC
100
100
LOEC
>100
>100
ChV
>100
>100
Method
Steel's
Dunnett's
Stats
Survival
Growth
Conc.
Critical Calculated
Critical Calculated
22.5
10 18
2.41 1.0135
45
10 18
2.41-0.7027
75
10 16
2.41 0.5743
90
10 16
2.41-1.3243
100,
10 16
2.41 0.5338

Meritech, Inc, (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
Client: FVTC # of Organisms per Chamber 10
NPDES #: 00665166 Test Vessel Size: 400 ml
Initiated by: � /
Date/Time Fed: 10/23/18
Date/Time Born: 10/22/18 s:ao PM CT
Organism Source: Aquatox, Inc.
Transferred by:
Date
Time Initials
Day 1
10/24
Day 2
10/25
Day 3
10/26
1--)
Day 4
10/27
Day 5
10/28
0'
Day
10/29
./- , (,x/
Test Solution Volume: 250 mi
Temp. of Stock: a � " °C
# of Reps: 4
Test Organism: Pimenhales promelas
Start Date: 10/23/18 Time:
End Date: 10/30/18 Time: I0
Randomization: Yes No
Incubator M
Analyst(s): MR, CD, LV, SIB
Fed by:
Date
Time 1 Initials
Time 2 Initials
Day 0
10/23
j7pr� Cyr)
Cl�
Day 1
10/24
r
7-S��rZ� ./
1
Day 2
10/25
-9fLqAtZ
v
Day 3
10/26
Day
10/27
".24_
1
Day 5 10 / 28�
Day 6 - 10/29 Liev)-
Test Termination Data:
Initials/Signature: A:7 " C
End Date: J0/30/18

Client: FVTC
ENVIRONMENTAL LABORATORIES
A plvlsI>n O'f Wztter Techr?a1,p_1-y and Conrrpls, Inc -
Chemical and Physical Determinations
NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Day
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwater
BatchN :
)
(�
pH: Initial
gf d
2
Final
}
b
9114
e7/nU-IQ
IMP
D.O.: Initial
"7
7 f o
• Vt jr'
' l?
%
Final
(a I]
Ub
PD
7, %
Temp.: Initial
1''(d_y
101
oC S r 9
2
Final
—,)-
9
Conductivity: Initial
j 0
Final
r
Residual Chlorine:
40, t
z . (
.1- 0 - (
2-6 , t
Z-0, I
G©
Hardness:
Alkalinity: 1
53
Day
Concentration: 22.50%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
-7, 0
—7, 7�,
17
1 j.
Final
Q r13
D.O.: Initial
f
Final
,
Temp.: Initial
oc `� 3
`t• 7
l (,
Final
o`Z . �
l�
� 715' oZ-
� �g
v� `HO
Conductivity: Initial
,9f
2(, %
,
.
Final
233
c3uG
Residual Chlorine: 1
Z p .• (
0. f I
G p, l
1 L o,
c.0 1
j
Day
Concentration: 45.0%
0-1
I-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
Cp
7 LU
Final
1, 2,
D.O.: Initial
`7
Final
Temp.: Initial
s
rj ��
'�j , rf
a
�,2` j -
IV,
- Final
2111
Conductivity: Initial
3
336
�
3S
'53
Final
Residual Chlorine:
G
GO ,
G p, 1
e0,

Client: FVTC
/WA=-"O7-A=- 4 r ff
IENVIROhIMENTAL LABQRP.TORIHS
Chemical and Physical Determinations
NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Dav
Page 2 of 2
Concentration: 75%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
49
4�
r3 r�r�
Final
q
� (,�
D.O.: Initial
9, Ha
36
b-
B
Ji")
Final
ri
Temp.: Initial
9q
�L1'
. C a
•
�L4,!�
Final
n
1121
,Q`t
Conductivity: Initial,LIU0
411
L) IS
Y)
Final
' 9
q ,
d
�ff)
Residual Chlorine:
1 /—0, (
L . ,
I Z-v
� f
q1 r
Dav
Concentration: 90%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
_ pH: Initial
�j
?tea
- 33 3
` -
Final
D.O.: Initial
, (� '
�)
0
Final
r,
l
p )-
3
12
_ 9
Temp.: Initial
1
�-;
9 c'
6948
S i ±
Final
.;%
,r
y
'J7, L1
9.
g1<)
Conductivity: Initial
)
q,ka
.tQG
L G
L4 -90
L
Final
14 -53
L11%
4U3
q
Residual Chlorine;
,
(
� G,
c Oa l
gyp, '
- t
Dav
Concentration: 100%
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
jj
)
�
.� 9 �
1
3
�,To
Final
(
j
D.O.: Initial
1
Final
tk
- HE
721DO
1 • 1O'
(Or
Temp.: Initial
15
51
Li.S
Oas•3
Final
gq, (
0
2NIS
, i�
a .
Conductivity: Initial
0
tc
Final
v
6
Residual Chlorine:
ZO,
LO • I
GO ,(
Od
LO.
GOr
GO- L
Hardness:
15-8
�1-1�1 -
Alkalinity:3
t
-

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 / _0 0—
Rep B 0
Rep C 0
Concentration 22.50%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 0 r
Rep B 0
07 0
Rep C 0 v
Rep D a.
Concentration 45.0%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 e� '
Rep B 0
Rep C 0—
C' V
Rep D 0

MERITECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 10/23/18
End Date: 10/30/18
Concentration 75%
Tuesday Wednesday Thursday
Friday
Saturday
Sunday
Monday
Tuesday
Rep A 0_
Rep B •0 ---E—
---tom-
Rep C 0
O
`�
Rep D 0__
Concentration 90%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0 / (9 n
Rep B 0_
Rep C 0_
Rep D 0
Concentration 100%
Tuesday Wednesday Thursday
Friday
Saturday
Su ay
Monday.
Tuesday
Rep A 0 �
�
�
_�•
0 _
Rep B 0
Rep C 0
Rep D 0_

~ ti ENVIRONNIEIVTAL LABORATORIES
A Pf WCA 6f Wafer"Tectinology aqd Conn-ols, 1-
FISH LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC
NPDES #: 0066516
Date of Test: 10/23/18
Initial weights taken.on:— - / by:
Final weights taken on: Jo- 31-17 by: IV
Page: 1 of 2
Outfall:
Organism: Pimephales promelas
CO NC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
## ORG.
MEAN
WTJORG. (mg)
SURV %
16.
0
A
E3.31
L!l('
lG
B
F3
U
F qo
o�
. on
;q7
q, (ol'Z
o, �1
o
- .
F� ), �Q
� 3
1 v
0, Q3
22.50%
A.
a�3 13
2 i �- 05?! 9
l
of �1 �6
(
B
J
R25'11l -3
3
of
7
Z So
- ,�o
0
/00
45.0%
.I
A
7
5,q 7
77
r}
, 7
I)
B
l -7, -66
Dab, 6
a
61 - 7
Milo)
c
-%
7
r.,

ENVIRONMENTAL LABORATORIES
i3 DivislCiri e! N�a te! 7echno/ygy and Con too/Si (nc_
FISH .LARVAL SURVIVAL/GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC
NPDES #: 0066516
Date of Test: 10123118
Initial weights taken on:��r15?by: bal
Final weights taken on: 10-31-11 by:
Page: 2 of 2
Outfall:
Organism: Pimephales prometas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
## ORG.
MEAN
WTJORG. (mg)
SURV %
p
75/o
A`
U >G
%
0
l 0
F0
C W
B
C][iJ,
7 ► J J
1 y
(O
o,
o 1 v
55
/0
-
Oe Fn
/a
90%
A
7f lKo
Q
Cis
10
B
(O
:J
!
�0
El 7, U q
W,1)6� z-1 3
7, �L
L2
0,-Z�q
)191--)
o
, "`'
5-
(?
Ci• L,'-2
A
C
0p
350
, vS
Ir

MERITECH, INC. MeritechSample lD#.
Bioassay Sample Chain of Custody
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(Mmeritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: TL-"vt PO#:
Contact Person: _ ^S- Civ. )v,� NPDES#: NC.GdC CtS- w
Address: i�GZ f� cc Phone:
City: Fv rr-, Pipe #:
County C, ky_ler State: Nc — Zip: ZZS7-Cp
SAMPLE INFORMATION
Sample Site: T�e'rf,6[r_ C<,L(C c4;Wti.-'-
Sample Type: ❑ Grab 0 Composite
Sampling Time:
Start Date:
Start Time: (SVJ
End Date: End Time: 0` W
# of containers: Z
ArI PM
A A PM
*** Triple rinse sample container with sample before filling. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0*C upon receipt t the laboratory***
Collector's Name: Print: Cz S Signature:
Test Required: MChronic (7 days)
❑ Acute (24-48 hours)
IWC: IL-) %
Comments:
TOXICITY TEST INFORMATION
Test Organism: Ceriodaphnia dubla (water ilea)
IAJ
Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
SHIPPING INFORMATION
Relinquished by: Date:Q • .'� 3 r
Received b Date:
Relinquished by: Date: / a
Received by: Date:
Relinquished by:
Received by:
Relinquished by:
Received by:
Time: 1! 3 U i PM
Tirge: i pPM
�_� i� PM
Time:
Time: AM PM
Date:
Time:
AM
PM
Date:
Time:
AM
PM
Date:
Time:
AM
PM
Date:
Time:
AM
PM
Sample Temperature CC):
Method of Shipment:❑ UPS ❑ Fed EX V Meritech Pick-up []Delivered ❑ Other.
VV
** Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED'*
SAS ►+-d3-i� SAMPLE -RECEIVING (Laboratory Use Only)
relinquished by:
jv
Received by: Date: w�lc ;} Time: AM
12 Sample Temperatures
WHITE = Laboratory copy YELLOW = Client copy

MERITECH; INC. Merltech Sample Ili#:
Bioassay Sample Chain of Custody
" 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed aMmeritech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: jt)ui ..� G� it J cU ( {�1 t".' l_ 1 PO#:
Contact Person: .; �t;/�cU t, s�- NPDES#: NCkk)(cb,) 1 W
Address: Phone:
City: Iwf( IJ u Pipe #:
County State: t v c.._ zip: 7-75 Z w
SAMPLE INFORMATION
Sample Site: Tew- .le—Cr,-<.c, -e�i!!✓'�
Sample Type: ❑ Grab 0 Composite
Sampling Time: Start Date: 10—Z14—ty
Start Time: (fd'>V J
# of containers: 2—
EndDate: to—i� C y End Time:
Al� PM
Alai PM
*** Triple rinse sample container with sample before rifling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory"*
Collector's Name: Print: 611-65 �'i'.i/rv� Signature:
Test Required: XChronic,(7 days)
❑ Acute (2448 hours)
Comments:
TOXICITY TEST INFORMATION
Test Organism: ❑. Ceriodaphnia dubla (water flea)
{Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
Test Concentrations (if multiple dilutions):
SHIPPING INFORMATION
Relinquished by: p Date:
Received b ���` Date: f 0 2Sr
Relinquished b Date:
Received by: Date:
Relinquished by:
Received by:
Relinquished by:
Date:
Date:
Date:
Time: ^/I � S
�PM
Time: �
AM PM
Time: J�a '
AM PNI
Time:
AM PM
Time:
AM PM
Time:
AM PM
Time:
AM PM
Received by: Date: Time: AM PM
Sample Temperature CC):
Method of Shipment: ❑ UPS ❑ Fed EX [ Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED •t
SAMPLE RECEIVING (Laboratory Use Only)
4inquished by:
Received by. Date: Time: &02rS' AM 40
Sample temperatures Cj J-.%_1 i s t I_ Sample Mull;
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC. MeritechSample IDM. to
Sloassay Sample Chain of Custody
-� 642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: [e-mail] Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: 'TQL,)k PO#.
Contact Person: kLH... NPDES#: NCW* (f(O Lb
Address c l 'T �� Phone:
City: u- Pipe #:
County: (n)i'� ��� State: Zip: i✓ZtrZt;,
SAMPLE INFORMATION
Sample Site: ��.<�J(t (_✓-�t�t- (..� ( I7
SampleType: ❑ Grab Composite # of containers:
Sampling Time: Start Date: Start Time: Ot! f.XJ Alyl PM
End Date: • IJ'2 "t End Time: e tW- Aiyf PM
fi
' ** Triple rinse sample container with sample before lling. Completely fill the ((sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receip t the laboratory'
Collector's Name: Print: C f� t- ✓% Signature:
TOXICITY TEST INFORMATION
Test Required: (A Chronic (7 days) Test Organism: ❑ Cerlodaphnia dubla (water flea)
❑ Acute (24-48 hours) Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
IWC: J % Test Concentrations (if multiple dilutions):
Comments:
SHIPPING INFORMATION
Relinquished by: Date: o vZ 6
Received W Date:
Relinquishedb �Date:
Received by: Date:
Relinquished by:
Received by:
Relingdished by:
Received by:
Date:
Date:
Date:
Date:
Sample Temperature CC):
Time: Zoa
Time:
Time: -5OS
Time:
Time:
Time:
Time:
Time:
AM
AM
AM PM
AM PM
AM PM
AM PM
AM PM
AM PM
Method of Shipment:❑ UPS ❑ Fed EX V Meritech Plck-up ❑ Delivered ❑ 'Other
** Samples shipped on Friday must'be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED *'
SAMPLE RECEIVING (Laboratory Use Only)
elinquished by:
Received by: _ Date: dY1 Time: f! .r AM
amp
Sample Temperatures C : s
e C n ion:
WHITE = Laboratory copy. YELLOW = Client copy

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Shapiro - Wilk's Test for Normality
----------------------------------------------------------------------------
D = 0.1518
W = 0.9743
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 3.3216 (p-value = 0.6505)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 15.0863 (alpha = 0.01, df = 5)
= 11.0705 (alpha = 0.05, df = 5)

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE DF SS MS F
----------------------------------------------------------------------------
Between 5 0.0424 0.0085 1.0065
Within (Error) 18 0.1518 0.0084
----------------------------------------------------------------------------
Total 23 0.1942
----------------------------------------------------------------------------
(p-value = 0.4422)
Critical F = 4.2479 (alpha = 0.01, df = 5,18)
= 2.7729 (alpha = 0.05, df = 5,18)
Since F < Critical F FAIL TO REJECT Ho: All equal (alpha = 0.05)
0
19

Title: FVTC
File:
FVTCGrowth
Transform: NO
TRANSFORMATION
<r Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
--------
- - ---
TRANSFORMED
MEAN CALCULATED
IN SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
T STAT 0.05
1
Control
0.9240
0.9240
2
22.50
0.8215
0.821.5
1.5785
3
45
0.8035
0.8035
1.8557
4
75
0.8150
0.8150
1.6786
5
90
0.8015
0.8015
1.8865
6
100
0.8365
0.8365
1.3475
-----------------------------------------------------------------------------
Dunnett
critical. value = 2.4100, (1 Tailed,
alpha = 0.05, df
= 5,18)
Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
MIN SIG DIFF
o OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
(IN ORIG. UNITS)
CONTROL
FROM CONTROL
Control
4
22.50
4
0.1565
16.9•
0.1025
'P3
45.
4
0.1565
16.9
0.1205
4
75
4
0.156.5
16.9
0.1090
5
90
4
0.1565
16.9
0.1225
6
100
4
0.1565
16.9
0.0875

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test Date1/17/2019
Facility: Fuquay Varina
Laboratory: Meritech Inc.
Signature of Operator in Re portsible Charge
x '1�1 / ;
Signature of Laboratory Supervisor
NPOES # NC00 66516
MAIL ORIGINAL TO: Water Sciences Section
Aquatic Toxicology Branch
Division of Water Resources
1621 Mail Service Center
Raleigh, N.C. 27699-1621
Test Initiation Date/Time 1/8/2019 4:33 PM
% Eff. Repl.
Control I Surviving #
Original #
Wt/original (mg)
22.5 Surviving #
Original #
Wt/original (mg)
45 Surviving #
Original #
Wt/original (mg)
75 Surviving #
Original #
)Wt/original (mg)
90 Surviving #
Original #
Wt/original (mg)
100 Surviving #
Original #
Wt/original (mg)
Quality Data
Control
pH (SU) Init/Fin
DO (mglL) Init/Fin
Temp (C) IniUFin
High Concentration
pH (SU) Init/Fin
DO (mg/L) [nit/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
p. at Receipt (*C)
2 3 4
Pipe #: County: Wake
Avg Wt/Surv. Control 0.724
10
9
9
10
10
10
10
10
0.683
0.748
0.587
0.728
9
9
10
10
10
10
10
10
0.776
0.711
0.683
0.658
10
10
8
1 10
10
10
10
10
0.614
0.794
0.533
0.658
10
9
10
110
10
10
10
10
0.790
0.638
0.616
0.684
10
10
10
10
10
10
10
10
0.775
0.565
0.679
0.664
10
10
10
9
10
10
10
10
0.785
0.585
0.709
0.657
% Survival . 95.0
Avg Wt (mg) 0.687
% Survival 95.0
Avg Wt (mg)0.707
% Survival 95A
Avg Wt (mg) 0.650
% Survival 97.5
Avg Wt (m
g).F 0.682
% Survivall 100.0
Avg Wt (mg) 1 0.671
% Survival 97.5
Avg Wt (mg) 0.684
Day
0 1 2 3 4 5 6
Test Organisms
i' Cultured In -House
Ps Outside Supplier
Hatch Date: 1/7/19
Hatch Time: 3:00 pm CT
8.01 1 7.85
7.98 / 7.81
8.23 / 8.12
18.26 / 8.08
8.28 1 7.98
8.22 1 7.99
8.24 / 7.71
7.71 1 6.95
7.29 / 7.04
17.87 l 7.26
7.76 / 7.74
8.15 l 7.53
8.15 1 7.41
1
7.89 / 6.55
1
24.5 1 25.2
24.6 1 24.9
124.0 / 24.0
125.2 / 24.3
24.5 1 24,7
24.4 / 24.1
24.6 / 24.2
7.41 / 7.97
7.54 / 8.03
7.44 / 7.99
7.80 / 8A4
7.89 1 7.92
T65 / 7.92
7.70 / 7.73
8.26 1 6.84
7.53 /, T25
8.15 / 7.10
7.68 ! 7.74
8.11 / 7.25
8.45 / 7.28
'8.20 / 6.32
25.1 / 26.8
24.3 / 25.0
25.7 ! 25.2
24.6 124.7
25.9 / 24.9
25.3 / 24.1
25.2 / 24.5
1 2 3
1/7/2019
. 1/9/2019
1110/2019
24.0
24.0
24.0
46
48
46
72
80
75
465
488
490
<0.1
<0.1
<0.1
1.8
1.8
1.9
Dilution H2O Batch # 1348 1349 1360 1351
Hardness (mg/L) 44 44 44 46
Alkalinity (mg/L) 50 55 52 54
Conductivity (umhoslcm) 193 208 236 217
Survival
Growth
Normal
Hom. Var,
F"1:
J?�
NOEC
100
100
LOEC
>100 -
>100
ChV
>100
>100
Method
Steel's
Dunnett's
Overall Result
ChV >100
Stats
Survival
Growth
Conc.
Critical Calculate_ d
Critical Calculated
22.5
10 18
2.41-0.3544
45
10 19
2.41 0.6354
75
10 20
2.41 0.0778
90
10 22
2.41 0.2723
100
10 20
2.41 0.0432

Client: FVTC
NPDES #: 0066516
I
Initiated by. p,- _
I
DatefTime Fed: 11/13/18
Date/Time Born: 11/12/18 3:00 PM Cr
Organism Source: Aquatox, Inc.
Transferred by:
Meritech, Inc. (Lab # 027)
Chronic Fathead Minnow Benchsheet
Transfer and Feeding Dates and Times
# of Organisms per Chamber:10
Test Vessel Size: 400 ml
Test Solution Volume: 250 ml
Temp. of Stock: °C
# of Reps: 4
Test Organism: Pimephales promelas
Date
Time Initials
Day 1
11/14Q�
y� ���(�/
Day 0
Day 2
11/15
`���"'�'�- �(,� Day 1
Day 3
11 / 16
s/i'/�� /
Day 2
Day
11/17
5-7>'
Day 3
Day 5
11/ 18
��ti C�
Day 4
Day 6
11/19
�(-�7'-
! jti%
Day 5
Day 6
Start Date: 11/13/18
End Date: 11/20/18
Fed by:
Time:
Time:
Randomization: Yes No
Incubator#:
Analyst(s): MR, CD, I-V, SB
Date
Time 1 Initials
Time 2 Initials
11/13
_a C✓
s;Q n 'i ce
11/14
7's(�i�e i
-
11/16
—Z
,� • 7 m C�
11/17
11 / 18
3_20pr ! •r-
11/19Q._._"
1
Test Termination Data: /
Initials/Signature:✓� C✓�yz,�lrlt I�,�M�/,
End Date: 11/20/18
1

G B S N N ALaw err I ffr / s M 'Wmw or
ENVIRONMENTAL LABORATORIES
A OIVI.sIon caf -pier "`Technology anct Controls, Inc.
Chemical and Physical Determinations
Client: FVTC NPDES#: 0066516 StartDate: 11/13/18
End Date: 11/20/18
lnav
Concentration: control
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
Soft Synthetic Feshwater
Batch # :
� 3 �
7
131
i l 11
1 3
i
t
��
PH:_ Initial
. 03
91 1
(T•- 07
Pit 9
9
It 99�
Al l
Final
T,,7
7
D.O.: - Initial
(Q�
06
Final
Temp.: Initials
�l U
Final
Ll�
�; �`i—
�k S
I
Conductivity: Initial
1
Final
o'Z
la
1
Residual Chlorine:
Z'-0. t
2.r)
1
2-O. (
GO •
�C7
u Hardness:
Lf
t'I
Alkalinity:
rj
�7
Dav
Concentration: 22.50%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
{ r 6
(L
�ft
L! y
W, 17
cin
Final'.
, C
�t7
D.O. Initial
-7,
Fugal
?
6�i �->
'-7.
iC .
Temp.: Initial
Final
r
a"�• i
r
�r�.
ya
-2I `�
�`E'
D ; '(
°`�►(
�527
Conductivity: Initial
;;� j
�3
�7
�t
a`
"7 s
Final
M /-
i �J(v
c �J
� �
�F
Residual Chlorine:
G-0"1
/-p r
G,Q,
ZZ,
� OI
Z-0i-
/-
Dav
Concentration: 45.0%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
"7,2
1O�
U3-
Final
D.O. Initial
, k
r
,s f
03
3
Final
. '3
,
�.
g
Temp.: Initial
o�
-5•
4 1 S
A �
_�
a5 �)
LL
Final
`� •
��
� • 0
R'L 0
a
Conductivity: Initial
g'l
7-
?
nc)
Final
b
b
3
Residual Chlorine:
4011
La.l
Z-O'
�

A&— M VL Ir ,ter .i- -Mr. w / wAp, Air - AV wW
ENVf:iOry NI@NTAI- LABCRATO�IES
A N<. — Yr. a/u.u/...py.NnN.C.irir-/s,, /r.c.
Chemical and Physical Determinations
Client: FVTC NPDFS#: 0066516 Start Date: 11/13/18
End Date: 11/20/18
Dav
Page 2 of 2
Concentration: 75%
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
-FinalD.O.:
076
InitialFinal
t
JTemp.:
Initial
.i
oZ�
.. `j�c
�H i
Final
a5• b
`1-
`�-�•`1
ak. 1
;,2�(<
Conductivity: Initial
i'�gL.
'jam 5
13 � 3
3
3
Final
3 f�
��
�o %
Residual Chlorine:
z Q, [
ZO, (
Z ,
Gv : (
Gd , f
Z-O 1
LU
Dav
Concentration: 90%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
�] �,
. % 6
, Y
q
6
177
Final
D.O,: Initial
�l�
op,
Final
40
7 CI
a, q�
►3
Temp.: Initial
94
�H r
��j,
��j'c
„?�t
a �]
Finalf
��
���'�,>
�'1 "i
o� 1t-r7
�`f.
Conductivity: Initial
4.
Final
5
q�
Residual Chlorine:
,
Lam,
LD,
L4•
Dav
Concentration: 100%
0-1
1-2
2-3
3-4
4-5
5-6
6-7
Remarks
pH: Initial
,
7?
r q
�
)
Final
��
U
�. %
D.O.: Initial
Final
Temp.: Initial
air
�`G1
�s 13
r
Final
lkl
,�S
-
oZ �
•
Conductivity: Initial
q,
-3
Final
4 qS
q
Residual Chlorine,
` ,ZO.
Z.0, <
LQ,
Hardness:
5
Alkalinity:
6
5,6

-M-E-RI-T-EC-H; INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 11/13/18
End Date: 11 /20118
Concentration Control
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
a
Rep B 0_ --— --_
Rep C 0
Rep D 0 O
Concentration 22.50%
Tuesday Wednesday Thursday Friday Sat Jday Sunday Monday Tuesday
Rep A 0
0-
Rep B 0 1`}_
Re C 0 �_ E
Rep D 0
Concentration 45:0%
Tuesday Wednesday Thursday
Friday Saturday
Sunday
Monday
Tuesday
Rep A 0—--
C/
Rep B 0
Rep C 0 r
`�
Rep 0 � �z 0 & o � —0

MERIT-ECH, INC.
Mortality Data: Chronic Fathead Test
Client: FVTC NPDES#: 0066516 Start Date: 11/13/18
End Date: 11/20/18
Concentration 75%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
RepA— c a
0 �.._ �7 - Rep B 0_ O0—
Rep
C 0
Rep D 0 `"` 0—
Concentration 90%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A 0
Rep B 0 % V
Rep C 0
Rep D 0 / --—
Concentration 100%
Tuesday Wednesday Thursday Friday Saturday Sunday Monday Tuesday
Rep A Q
Rep B 0�
Rep C 0_
Rep D 0

ENV{RONM,EN7AL LABORAT.ORIE5:.
' � Dfiflsjon Qf lNa }erTQrhnoltigy and•Concre�l3Jnc '
FISH LARVAL SURVIVAL(GROWTH DATA SHEET
CHRONIC FATHEAD MINNOW .
Facility: FVTC
NPDES #: 0066516
Date of Test: 11 /13/18
Initial weights taken on: 11-,�L- by:�
Final weights taken on: 0-1'l by: (A-1
Page: 1 of 2
Outfall:
Organism: Pimephales promelas
GONG
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WTJORG. (mg)
SURV %
A
o.3
��S
Cif 50
0
L.
G
O
OV63
4
F370
7, ,
0z7 _
l o o
D
q.-30
3o?.3
/0 1
O 3l
�
A
gC.(
3q.0
�.
oo
B
Fa fl
��3
0
O�o �
00
°
22.50 /o
C+
/ YL-
01•
00
I3
Q,�
lool
45.0%
c
wgq.z5
IM
10
n, 7qq
o

NviRPNNI�ZVT+AF:,L. -B•ORATO.Ries;
`s< " :A'Dipis�dtl eFt%Vaie�TeYtinDrvgy:an,dCon tro%s, yqr.,
FISH LARVAL SURVIVALIGROWTH DATA SHEET
CHRONIC FATHEAD MINNOW
Facility: FVTC Page: 2 of 2
NPDES #: 0066516
Date of Test: 11/13/18
Initial weights taken on: by:
Final weights taken on: / /. t� i by:. A
Outfall:
Organism: Pime hales promelas
CONC
REP
PAN WT.
(mg)
PAN + ORG.
WT. (mg)
WT. OF ORG.
(mg)
# ORG.
MEAN
WT./ORG. (mg)
SURV %
A
z3a
3�f�00
^7, l
d J7I
90 %
A
L a
`� /
0, -7/7
/,0-0
g
F , (o
6,
l
o,.
C
i
35
r
D
�7p�, 01
5 -3
yr 1,
V
'100%
A
3 to ,!
8 .
do
B
70
lO
, Z
O
C+
f1
►66-7
/00.
D
��
1

ME.RITECH, INC.
Meritech Sample ID M
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike.reed(cbmeritech-labs.com _ Web Site; www.meritech4abs.com
r CLIENT INFORMATION
Client: 0 � n D( FtN U M --L 1 40401
Contact Person:
Address:
City:
PO#: .
NPDES#. NO 00640C
Phone:
Pipe#: toi
County: State: Zip:
SAMPLE INFORMATION
Sample Site: ��� t'Five-n�
Sample Type: ❑ Grab Composite # of containers: 2.
Sampling Time. . Start Date: j.( -I ?--I T, Start Time: 0900 11� PM
End Date: i ^ (3 -(� End Time: 0 9c3"cT l AA PM
"** Triple rinse sample 'contalner with sample before filling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory"**
Collector's Name: Print: -T-- S Signature:
Tpst Required: Y`-` Chronic (7 days)
❑ Acute (24-48 hours)
iWC: v ono
Comments:
TOXICITY TEST INFORMATION
Test Organism: ❑ Ceriodaphnia dubia (water flea)
(1 Pimephales promelas (fathead minnow)
❑ Mysidopsis bahla (shrimp)
Test Concentrations (if multiple dilutions):
SHIPPING INFORMATION
Relinquished by: Date: ��/�� `e'Time: M PM�
Received by: Date: / ��13 3 Time: b ' r PM
i 1!
Relinquished by: Date: (L - (� ` Time:' S AM P-7m
Received by: Date: Time: AM PM
Relinquished by:
Received by:
Relinquished by:
Date: Time: AM PM
Date:
Date:
Time: AM PM
Time: AM PM
Received by: Date: . Time: AM PM
Sample Temperature CC):
Method of Shipment ❑ UPS ❑ Fed EX 0 Meritech Pick-up ❑ Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED •`
SAMPLE RECEIVING (Laboratory Use Only)
Ainquished by: q&:=�
Received by: Date: y Time: . _ AM 6
Sample Temperatures (°C): / ' `i' / I Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC.
Merltech Sample ID #: I + i 4W`�
WE
642 Tamco Rd, Reidsville NC 27320
;�+� Phone: [phone] Fax:[phone]
1 Toxicity Supervisor email: mike.reed(d-meritech-labs.com_ Web Site: www.meritech-labs.com
CLIENT INFORMATION
Client: 'Tout, dt fufUk-f UT'7
Contact Person: <H s
Address: 5"�t2 bl3up xZ
City:o��
County: (JjZ C-C State: NL
SAMPLE INFORMATION
PO#:
NPDES#: NCtluf.6T� tto
Phone:
Pipe #: W 4
Zip: Z?,$` t t, -
Sample Site:
Sample Type: ❑ Grab Composite # of containers: 2-
Sampling Time: Start Date: Y StartTime: .0100 AA PM
End Date: End Time: 0?Jb A0 PM
*** Triple rinse sample container with sample before riding. Completely fill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < 6.0°C upon receipt at the laboratory'
Collector's Name: Print: ckr:5 Signature: (Je" �IeL
Test Required: Chronic (7 days)
❑ Acute (24A8 hours)
IWC: 9a %
Comments:
TOXICITY TEST INFORMATION
Test Organism: ❑ Ceriodaphnia dub/a (water flea)
0 Pimephales promelas (fathead minnow)
❑' Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
SHIPPING INFORMATION
Relinquished by: �, � Date: l — lr` q
Received by: �'��Date: .s
Relinquished by: Date:
Received by: Date:
Relinquished by:
Received by:
Relinquished by:
Received by:
Date:
Date:
Date:
Date:
Sample Temperature (°C):
Time: '02/' J�
Time:
Time:
Time:
Time:
Time:
Time:
Time:
AM 60
AM
AM
AM PM
AM PM
AM PM
AM PM
AM PM
Method of Shipment:❑ UPS ❑ Fed EX 0 Meritech Pick-up ❑Delivered ❑ Other
" Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED
SAMPLE RECEIVING (Laboratory Use Only)
inquished by:
Received by: [f Date: Time: (60 q U AM
Sample Temperatures (°C): r —t / / / Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

MERITECH, INC.
Merilech Sample ID #: II I �EJ_ (I ty)
642 Tamco Rd, Reidsville NC 27320
Phone: [phone] Fax:[phone]
Toxicity Supervisor email: mike. reed(&merifech-labs.com Web Site: www.meritech-labs.com
CLIENT INFORMATION
� PO#•
Client: �O(�u of }-ck/tiu �/ (c)li}7`�
Contact Person: ♦ Sf�([•cw}�� NPDES#: NC00" ISfa
Address: SD• l Phone:
City: Pipe #:
FiX�rJc•,.-t' '
County: f 4 t& State:_ Zip: Z��l
Sample Site:
Sample Type: ❑ Grab Composite
SAMPLE INFORMATION
# of containers: _ _ T_
Sampling Time: StartDate: Q-15--181 StartTime: 6 ivl% A0 PM
End Date: (I- tp, -i, is End Time: oi,, C, J A0 PM
'*• Triple rinse sample container with sample before tilling. Completely rill the sample container with no air space.
Pack the sample cooler completely in ice. The sample must be < B.00C upon receatthelaborato
Collector's Name: Print: Signature:
_Test Required: � Chronic (7 days)
❑ Acute (24-48 hours)
IWC: 6 %
Comments:
TOXICITY TEST INFORMATION
Test Organism: ❑ Ceriodaphnia dubia (water flea)
[ Pimephales promelas (fathead minnow)
❑ Mysidopsis bahia (shrimp)
Test Concentrations (if multiple dilutions):
-_ SHIPPING INFORMATION
Relinquished by: (J""" ��/`" Date: If- t4 -' �
Received by: •�� Date: •-� - {
Relinquished 1 Date:=�
Received by: Date:
Relinquished by:
Date:
Received by:
Date:
Relinquished by:
Date:
Received by:
Date:
Sample Temperature (°C):
Time: / AM P
Time: AM PM
Time: AM M
Time: AM PM
Time: AM PM
Time: AM PM
Time: AM PM
Time: AM PM
Method of Shipment: ❑ UPS ❑ Fed EX Meritech Pick-up ❑ Delivered ❑ Other
'• Samples shipped on Friday must be FedEx and must be clearly labeled for Saturday delivery, NO SIGNATURE REQUIRED'*
SAMPLE RECEIVING (Laboratory Use Only)
r
ainquished by: ),c
Received by: Date: Time:_ AM -@�:)
Sample Temperatures ( Sample Condition:
WHITE = Laboratory copy YELLOW = Client copy

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
- Shapiro - Wilk's Test for Normality
------------------------------------------------------------------------
D = 0.0502
W = 0.9616
Critical W = 0.8840 (alpha = 0.01 , N = 24)
W = 0.9160 (alpha = 0.05 , N = 24)
Data PASS normality test (alpha = 0.01). Continue analysis.

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Bartlett's Test for Homogeneity of Variance
----------------------------------------------------------------------------
Calculated B1 statistic = 7.5823 (p-value = 0.1808)
Data PASS B1 homogeneity test at 0.01 level. Continue analysis.
Critical B = 15,0863 (alpha = 0.01, df = 5)
11.0705 (alpha = 0.05, df = 5)

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
ANOVA Table
----------------------------------------------------------------------------
SOURCE
DF
SS
MS F
----_-^_--------
Between
------
5
0.0204
0.0041 1.4623
Within (Error)
18
0.0502
0.0028
----------------------------------------------------------------------------
Total
23
0.0706
-------------------------------------_--_-----------------------------------
(p-value = 0.2507)
Critical F = 4.2479
= 2.7729
Since F < Critical F
( alpha = 0.01, df = 5,18)
(alpha = 0.05, df = 5,18)
FAIL TO REJECT Ho: All equal (alpha = 0.05)

Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 1 OF 2
Ho:Control<Treatment
----------------------------------------------------------------------------
TRANSFORMED
MEAN CALCULATED IN
SIG
GROUP
IDENTIFICATION
MEAN
ORIGINAL UNITS
T STAT 0.05
---
-----
1
--------------------
Control
-----------
0.7793
------------------
0.7793
------
2
22.5
0.7345
0.7345
1.1986
3
45
0.6915
0.6915
2.3502
4
75
0.7248
0.7248
1.4597
5
90
0.7123
0.7123
1.7945
6
100
0.6968
0.6968
2.2096
----------------------------------------------------------------------------
Dunnett critical value = 2.4100 (1 Tailed,
alpha = 0.05, df =
5,18)
Title: FVTC
File: FVTCGrowth Transform: NO TRANSFORMATION
Dunnett's Test -
TABLE 2
OF 2 Ho:Control<Treatment
----------------------------------------------------------------------------
NUM OF
MIN SIG DIFF
a OF
DIFFERENCE
GROUP
IDENTIFICATION
REPS
(IN ORIG. UNITS)
CONTROL
FROM CONTROL
-----
--------------------
Control
-------
4
----------------
-------
------------
22.5
4
0.0900
11.5
0.0448
3'
45
4
0.0900
11.5
0.0878
4
75
4
0.0900
11.5
0.0545
5
90
4
0.0900
11.5
0.0670
6
----------------------------------------------------------------------------
100
4
0.0900
11.5
0.0825