NC0024911_Tox Lab Reports_20211221

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vw.p�Whson www.paeelabs.enm
November 23, 2021
Mr. Roger Edwards
Metropolitan Sewerage District
2028 Riverside Drive
Asheville, NC 28804
RE: Project: Bioassay - NC0024911 11/1
Pace Project No.: 92569736
Dear Mr. Edwards:
Pace Analytical Services, LLC
2225 Riverside Dr.
Asheville, NC 28804
[phone]
Enclosed are the analytical results for sample(s) received by the laboratory between November 01, 2021 and November
05, 2021. The results relate only to the samples included in this report. Results reported herein conform to the applicable
TNI/NELAC Standards and the laboratory's Quality Manual, where applicable, unless otherwise noted in the body of the
report.
Some analyses were subcontracted outside of the Pace Network. The test report from the external subcontractor is
attached to this report in its entirety.
The test results provided in this final report were generated by each of the following laboratories within the Pace Network:
If you have any questions concerning this report, please feel free to contact me.
Sincerely,
Lorri Patton
[e-mail]
1(828)254-7176
Project Manager
Enclosures
cc: Ms. Shannon Bergeron, Metropolitan Sewerage District
Mr. John Gosnell, Metropolitan Sewerage District
Mr. Chad Ledford, Metropolitan Sewerage District
Mr. Dwayne Martin, Metropolitan Sewerage District
Ms. Cassandra McKnight, MSD of Buncombe County
Patrick Smith, MSD of Buncombe County, NC
Mr. Alan Taylor, Metropolitan Sewerage District
REPORT OF LABORATORY ANALYSIS
This report shall not be reproduced, except in full,
without the written consent of Pace Analytical Services, LLC. Page 1 of 16

Laboratory receiving samples:
Asheville
Edenr Greenwood ❑
Sample Condition
Upon Receipt
Courier:
Commercial
Client Name:
❑Fed Ex ❑UPS
['Pace
Document Name:
Sample Condition Upon Reeeipt(SCUR)
Document No.:
F-CAR-CS-033•Rev.07
Huntersville ❑ Raleigh❑
Proles
❑LISPS Client
❑Other:
Custody Seal Present? Dyes Qfgo Seals Intact?
Packing Material: ['Bubble Wrap ['Bubble Bags
Thermometer:
IR Gun:D:
Cooler Temp:
qr 3
Des to
Pone ❑. Other
et ❑Blue
Type of Ice:
Correction Factor:
Add/Subtract ('C)
Cooler Temp Corrected ('C):
USDA Regulated Soil (❑ N/A, water sample)
Did samples originate in a quarantine zone within the United States: CA, NY, or SC (check maps)?
y3
Document Revised: October 28, 2020
Page Iof2
Issuing Authority:
Pace Carolinas quality Office
Mechanicsville❑ Atlantan] Kernersville❑
WO#:92569736
111111IIIIIIIIIIIIIIII
Date/Initials Person ExaniiningContents: ✓' /r i/`
❑Nose
Biological Tissue Fr zen?
Lives ❑No a/A
Temp should be above freezing to 6°C
❑samples out of temp criteria. Samples on ice, cooling process
has begun
Did samples originate from a foreign source (internationally,
rn�r f rr Hawaii and Puerto Ricoi? ❑Yes No
l_JYes LJrvo
.__
Comments/DiSCrepancyA,
Chain of Custody Present?
Yes
❑No
❑N/A
1•
Sarrsples Arrived within Hold Time?
es
❑No
❑N/A
2,
Short Hold Time Analysis (<72 hr,)?
es
❑No
DNA
3.
Rush Turn Around Time Requested?
❑xes
❑No
❑N/A
.
Sufficient Volume?
es
❑No
❑N/A
5.
_
Correct Containers Used?
-Pace Containers Used?
, res
s
❑No
❑Na
❑N/A
j,,,,,
6.
Containers Intact?
Tries
❑Na
ON/A
7•
Dissolved analysis: Samples Field Filtered?
❑ves
❑No
{'iNlWA
8.
Sample Labels Match COO
-Includes Date/Time/ID/Analysis Matrix: _
s
1/1-77
❑No
❑N/A
9,
Headspace in VOA Vials (>5-6mm)?
pees
❑No
)t<!/A
10,
Trip Blank Present?
Trip thank Custody Seals Present?
Des
Des
❑No
Om
,/A
t/A -_
11•
COMMENTS/SAMPLE DISCREPANCY
Field Data Required? ❑Yes ❑No
Lot ID of split containers:
CLIENT NOTIFICATION/RESOLUTION
Person contacted:
Project Manager SCURF Review:
Date/T]rn0:
Date:
Project Manager SRF Review:
Date:
Page 2 of 16

a
/2eAnalyt!caf
Document Name:
Sample Condition Upon Receipt(SCUR)
Document Revised: October 28, 2020
Page 2 of 2
Document No.:
F-CAR-CS-O33-Rev.07
Issuing Authority:
Pace Carolinas Quality Office
*Check mark top half of box if pH and/or dechlorination is
verified and within the acceptance range for preservation
samples.
Exceptions: VOA, Coliform, TOC, Oil and Grease, ORO/8015 (water) 00C, LLHg
**Bottom half of box is to list number of bottles
Project WO# : 92
PM: LJP Due Date: 11/15/21
CLIENT: 93—MSD
tx
E
g
SP4U-12S mL Plastic Unpreserved (N/A) (CI-1
BP3U-250 mL Plastic Unpreserved (N/A)
113P2U-SOO rnL Plastic Unpreserved (N/A).
ti
a
'Z.'
Z
m
a
C
D
I
e`
,t
j
c
m
BP45-125 mL Plastic 1-12SO4 (pH < 2) (CI-)
SP3N-250 mL plastic HNO3 (pH <2)
BP4Z-125 mL Plastic ZN Acetate & NaOH (>9)
BP4C-125 mL Plastic NaOH (pH > 12) (CI-)
WGFU-Wide-mouthed Glass jar Unpreserved
AG1U-1 liter Amber Unpreserved (N/A) (CI-)
AG11-i-1 liter Amber MCI (pH < 2)
AG3U-25O mL Amber Unpreserved (N/A) (CI-)
v
[1
On
N
5
"
E
f
m
,
4Tcn
t7
a
AG3S-250 mL Amber H2SO4 (pH < 2)
AG3A(DG3A)-250 mL Amber N114CI (N/A}(CI-)
6
_
U
y
O
>
c
7
a
VG9T-40 mL VOA Na2SZO3 (N/A)
d
Z
C
:
0
}
E
R
7
l�7
?
DG9P-40 mL VOA H3PO4 (N/A)
.
.
M
�`'
x
ID
s
j
'f
4
>
V/GK (3 vials per kit)-VPH/Gas kit (N/A)
SPST-12S rnL Sterile Plastic (N/A— lab)
SP2T-250 mLSterile Plastic (N/A —lab)
n
al
w
O
r,
T
2
.:
a
-
n
N
a
m
AGOU-100 mL Amber Unpreserved vials (N/A)
VSGLI-20 rnL Scintillation vials (N/A)
DG9U-10 mi. Amber Unpreserved vials (N/A)
\\\\
3
milk%
5
\\L
\
h.
_
LikkIlL
Ilk
\
11111.
go
11
12lik,
.a.Klialk
II
ih.
xImik
\
�.
I _
\
\
pH Adjustment Log for Preserved Samples
Sarnple ID
Type of Preservative
pH upon receipt
Date preservation adjusted
Time preservation
adjusted
Amount of Preservative
added
Lot ft
Off
Note: Wheneve there is a discrepancy affecting North Carolina compliance samples, a copy or nis farm wsh ce sent to the north Carolina DEHNR Ceruncaunn sci {La.
Out of hold, incorrect preservatve, out of temp, Incorrect containers.
Page 3 of 16

COMPOSITE SAMPLING PROCEDURES
Composite samples must be collected over a 24 hour period.
Time Proportional: 1 sample each hour for 24 hours. Equal volui.
or at minimum 1 sample every 4 hours over 24 hours.
Plow Proportional: As per instructions in NPDES permit.
special Instructions:
Sample Custody Transfer Record Secure Receipt Sample
Date Time Relinquished By / Organization Received By / Organization Area Tema 'C Preserved?
rn
0)
m c)
N
(0) Grab (C) Composite
�'
il-
y
1
T....
V
_
n
W
'�
n 12
.
o
y p
5 ILI
O Fri
n o
CA
.Z
)
(Grab or Composite)
!Simple Collection Dnle l Time
..0
��f
TEMPERATURE MONITORING PROCEDURES
Sample temperature during collection and transport must be between
0.0 and 6.0'C. Samples must not be frozen. Use water ice in sealed bags.
J
q
n21- �
2s
Sign, and Print below
the dotted line
Collected by
Clean Water Act ,,o
Stormwatcr o
un
Sediment g
Non -Regulatory
# of Containers
Plastic o
Glass N.
~-rs,
,.._
_
1
r
r
i-
Volume
n
'~'
-
Ice
-*
11 N , if ti
oP znti
n
Acute Pass/rail
r
Acute Multi-Conc,
C
Chronic Pass/Fail
a
b
C
7
Chronic Multi-Conc.
t7
•
C. dubia
w-,
3
D. ambigua
.1
M
HOLD TIME PROCEDURES
For toxicity testing the sample must first be used within 36 hours
of sample collection (completion of composite sample).
Sample may not be used after 72 hours from sample collection.
.
Fathead Minnow
a
p
o
.R
Ivlysitl Shrimp
e.
,
Silverside
a
liyallela
'^
ro
Cliirotionlns
;1,
TIE/PRE
WER
a
Macroinveriebrate ID
0
m
3
n;y\
w rN
Y
O .
m
:,.7
ITT ASSIGNED I.AB ID II
(R3103)31a MIOIS.LDD AO MIVH3
age 4 of 16

EI, 1 IL 1
��v ron manta : Inc.
064) 677-6 42 . FAY 064) 877-693e
P.O. Box16414., GireenyIle_ 5C 4 Craftsrnan Court, Greer, SC 29650
Fathead Minnow Survival and Growth Test
EPA-821-R-02-013 Method 1000
Permit Renewal Second Species Testing
Client: MSD OF BUNCOMBE COUNTY
Facility: WRF
NPDES #: NC00249 11
Test Date: 02-Nov-21
Laboratory Sample ID #: T60518 P PROMELAS
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
President
Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples.
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification # 022
Page 1 of 6
Page 5 of 16

Effluent Toxicity Report Form -Chronic Fathead Minnow Multi -Concentration Test
Facility: MSD OF BUNCOMBE CO. NPDES # NC0024911
Pipe #: 001 County: Buncombe
Laboratory:
x
ETT Environmental, Inc.
Signature of Operator in Responsible Charge
x
Signature of Laboratory Supervisor
Comments
MAIL ORIGINAL TO:
Environmental Sciences Branch
Division of Water Quality
NC DENR
1621 Mail Service Center
Raleigh, NC 27699-1621
Test Initiation Date/Time 11/02/21
Eff.
Control
Repl.
Surviving #
Original #
Wt/original (mg)
3.0
Surviving #
Original #
Wt/original (mg)
6.0
Surviving #
Original #
Wt/original (mg)
12.0
Surviving #
Original #
Wt/original (mg)
24.0
Surviving #
Original #
Wt/original (mg)
48.0
Surviving #
Original #
Wt/original (mg)
Water Quality Data
Control
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
High Concentration
pH (SU) Init/Fin
DO (mg/L) Init/Fin
Temp (C) Init/Fin
Sample
Collection Start Date
Grab
Composite (Duration)
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
Chlorine(mg/L)
Temp at Receipt ( C)
Dilution H2O
Hardness (mg/L)
Alkalinity (mg/L)
Conductivity (umhos/cm)
1
/ 03:15 PM
2 4
4
Avg Wt/Surv. Control
10
10
9
9
10
10
10
9
0.6460
0.6110
0.6440
0.7656
10
10
10
9
10
10
10
9
0.6660
0.6180
0.6630
0.6744
10
8
10
10
10
9
10
10
0.6880
0.8333
0.6770
0.6660
10
10
10
9
10
10
10
9
0.6960
0.6340
0.6880
0.4889
10
10
10
9
10
10
10
9
0.7150
0.6630
0.6530
0.7622
10
10
10
10
10
10
10
10
0.7750
0.7320
0.6830
0.7850
Day
% Survival
Avg Wt (mg)
% Survival
Avg Wt (mg)
% Survival
Avg Wt (mg)
% Survival
Avg Wt (mg)
% Survival
Avg Wt (mg)
% Survival
Avg Wt (mg)
0.6845
97.4%
0.6666
100.0%
0.6554
97.4%
0.7161
100.0%
0.6267
100.0%
0.6983
100.0%
0.7438
Test Organisms
Cultured In -House
Outside Supplier
Hatch Date: 11/01/21
Hatch Time: 12:00 AM
0
1
2
3
4
5
6
7
7.8
/ --
7.8
/
7.6
7.7
/
8.1
7.8 /
7.8
7.8
/
7.7
7.8
/
8.0
7.8
/
7.6
----/
7.7
7.6
/ --
7.3
/
6.1
7.9
/
7.2
7.5 /
6.2
7.5
/
6.4
7.4
/
6.5
7.5
/
5.9
----/
7.5
24.7
/ --
24.5
/
24.9
24.6
/
24.8
24.5 /
24.2
24.7
/
25.5
24.8
/
25.4
24.6
/
25.6
---- /
25.5
0
1
2
3
4
5
6
7
7.9
/ --
8.1
/
7.9
8.1
/
8.0
8.3 /
7.8
7.7
/
7.7
7.9
/
7.8
8.0
/
7.6
----/
7.6
8.1
/ --
6.7
/
6.4
8.3
/
7.3
7.1 /
6.1
8.1
/
6.0
6.5
/
5.8
6.4
/
5.5
----/
5.5
25.0
/ --
24.6
/
24.9
24.7
/
24.8
24.6 /
24.2
25.1
/
25.5
24.9
/
25.4
25.2
/
25.6
---- /
25.5
1
2
10/31/21
11/02/21
11/04/21
24 hr
24hr
24 hr
68.8
85.4
187.5
0.0
0.0
0.0
416
435
461
<0.05
<0.05
<0.05
2.3
0.6
3.2
85.4
58.2
318
Normal
Hom. Var.
NOEC
LOEC
ChV
Method Steel's Test T-Test
Survival
no
yes
48.0%
Growth
yes
yes
48.0%
>48.00% >48.00%
>48.00% >48.00%
Overall Result
ChV
>48.00%
Stats Survival Growth
Conc. Critical Calculated Critical Calculated
3% 10.00 20.00 2.41 0.25
6% 10.00 17.50 2.41 -1.08
12% 10.00 20.00 2.41 0.87
24% 10.00 20.00 2.41 -0.69
48% 10.00 20.00 2.41 -1.68
DWQ Form AT-5 (1/04)
Page 2 of 6
Page 6 of 16

Day 1
Dead Lost
Day 2
Dead Lost
Day 3
Dead Lost
Day 4
Dead Lost
Day 5
Dead Lost
Day 6
Dead Lost
Day 7
Dead Lost
Weight
mg
Fecundity
N-Grav Gravid
source
rep
Survival
control
A
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.46
10
B
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.11
10
C
0
0
0
0
0
0
1
0
0
0
0
0
0
0
6.44
9
D
0
0
0
0
0
0
0
0
0
0
0
0
0
1
6.89
9
E
0
F
0
G
0
H
0
3
A
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.66
10
B
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.18
10
C
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.63
10
D
0
0
0
0
0
0
0
0
0
0
0
0
0
1
6.07
9
E
0
F
0
G
0
H
0
6
A
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.88
10
B
0
0
0
0
1
0
0
0
0
0
0
0
0
1
7.5
8
C
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.77
10
D
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.66
10
E
0
F
0
G
0
H
0
12
A
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.96
10
B
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.34
10
C
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.88
10
D
0
0
0
1
0
0
0
0
0
0
0
0
0
0
4.4
9
E
0
F
0
G
0
H
0
24
A
0
0
0
0
0
0
0
0
0
0
0
0
0
0
7.15
10
B
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.63
10
C
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.53
10
D
0
0
0
0
0
0
0
0
0
0
0
0
0
1
6.86
9
E
0
F
0
G
0
H
0
48
A
0
0
0
0
0
0
0
0
0
0
0
0
0
0
7.75
10
B
0
0
0
0
0
0
0
0
0
0
0
0
0
0
7.32
10
C
0
0
0
0
0
0
0
0
0
0
0
0
0
0
6.83
10
D
0
0
0
0
0
0
0
0
0
0
0
0
0
0
7.85
10
E
0
F
0
G
0
H
0
Renew
JG
JZ
AM
AM
JG
JG
End Date
Fed
JG
JZ
AM
AM
JG
JG
Time fed & renew
02'.55 PM
03'.43 PM
01 21 PM
11 31 AM
11 50 AM
11 45 AM
I 09-Nov-21 JZ
Effluent New temp. ve
24.6
24.7
24.6
25.1
24.9
25.2
04.03 PM
Old temp 'c
24.9
24.8
24.2
25.5
25.4
25.6
25.51
Control New temp. 'C
24.5
24.6
24.5
24.7
24.8
24.6
Lab#
T60518 P PROMELA
Client
MSD
Sample ID
WWTP
NPDES#
NC0024911
County
Buncombe
Month
11
Start & fed Date
02-Nov-21
Start & fed Time
03:15 PM
Started & fed By
AM
Test Organism
Pimephales promela:
Neo. born date
01-Nov-21
Neo. born time
1130-1300
Test Type
NCCD
Dilution Water
MHSF
Units for Conc.
%
%3rd BROOD
Test vessels
400 ml
Test volume
150 ml
Incubator #
1
Light
161t/8dk
Initial Temp°C
25
Artemia
.015g 2XDAY
Test method
EPA 821-R-02-013'.1000
Comments
All reps have 10
organisms unless noted otherwise.
Control ini temp
24.7
D=Dead N/A -Lost or not used
Page 3 of 6
Page 7 of 16

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131
6)
(Grab or Composite)
:13-
Sample Collection Data
1)
(Composite only)
E
1-
0
imNorl woo (0) cpi.z0 (D)
ge o
(13
c.
Sample Custody Transfer Record
0
N
13)
0
1313
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0
a.)
E
(41
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7-;*
TEMPERATURE MONITORIATG PROCEDUj
13
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134-413
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Et. (664) B77-0942 . FAY 064) 1}77-6930
P.O. Box 16414, Greenville, BC 28606
4 Craftsman Court, Grier, SC 291350
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: MSD OF BUNCOMBE COUNTY
Facility: WRF
NPDES #: NC0024911
Test Date: 02-Nov-21
Laboratory ID #: T60518 C dubia
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
President
•
Certification #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples.
Page 1 of 6
Patrick D. Timms
QA/QC Officer
SCDHEC Certification #23104
NCDENR Certification # 022
Page 11 of 16

Effluent Toxicity Report Form - Chronic Pass/Fail and Acute LC50
Date 18-Nov-21
Facility: MSD OF BUNCOMBE CO. WRF NPDES#NC0024911 Pipe# 001 County: Buncombe
Laboratory Performing Test: ETT Environmental, Inc.
Comments
X
Signature of Operator in Responsible Charge
X/
Signature of Laboratory Supervisor
MAIL ORIGINAL TO
North Carolina Ceriodaphnia Chronic Pass/Fail Reproduction Toxicity Test
CONTROL ORGANISMS
# Young Produced
Adult (L)ive (D)ead
Effluent %
2
3
4
5
6
7
8
9
10
11
Environmental Sciences Branch
Div. of Water Quality
N.C. DENR
1621 Mail Service Center
Raleigh, North Carolina 27699-1621
12
Chronic Test Results
Calculated t=
Critical Value=
% Reduction=
0.3419
2.508
1.2%
22
21
22
18
22
20
23
20
21
22
21
25
% Mortality
Avg. Reprod.
L
L
L
L
L
L
L
L
L
L
L
L
12.0%
TREATMENT 2 ORGANISMS
# Young Produced
Adult (L)ive (D)ead
pH
Control
Treatment 2
D.O.
Control
Treatment 2
1st sample
2
3
4
5
6
7
8
9
10
11
12
0%
Control
21.4
Control
0%
Treatment 2
21.2
Treatment 2
Control CV
8.1%
23
19
21
22
18
22
21
24
20
19
22
23
L
L
L
L
L
L
L
L
L
L
L
L
% 3rd Brood
PASS FAIL
100% I X I
7.6
8.1
7.8
8.2
start end
1st sample
7.6
8.3
7.7
8.4
1st sample
7.7
8.5
8.1
8.6
start end
1st sample
7.9
8.4
8.1
8.3
2nd sample
7.7
8.3
7.8
8.3
start end
2nd sample
8.0
8.6
7.9
8.2
Complete This for Either Test
Collection (Start) Date
Sample 1 31-Oct-21
Test Start Date
02-Nov-21
Sample 2 02-Nov-21
Sample Type (Duration)
Sample 1
Sample 2
Grab Comp Duration
24hrs.
24hrs.
1st 2nd
Tox Tox
Dilution Sample Sample
Hardness (mg/L)
Spec. Cond. (pmhos)
Chlorine (mg/L)
Sample Temp. at receipt (°C)
85.4
318
527
546
<0.05 <0.05
2.3
0.6
LC50/Acute Toxicity Test
(Mortality expressed as %, combining replicates)
Concentration
Mortality
LC50 =
95% Confidence Limits
Method of Determination
Moving Average
Spearman Karber
Pro bit
Other
Organism Tested
DEM Form AT-1
Ceriodaphnia dubia
i
start/end start/end
Control
High Conc.
pH D.O.
Page 2 of 6
Page 12 of 16

STATISTICAL ANALYSIS RESULTS
Facility:
MSD
NPDES# NC0024911
Sample ID:
NA \\ 1 P
ETT# T60518 C dubia
Date:
02-Nov-21
Laboratory:
ETT Environmental, Inc.
Certification #: NCO22
Exp. Date:
11/1/2022
Survival Data
7 Day Survival
Test Used: FISHERS TEST
Control
100%
Test Statistic: P=
1.000
Effluent
100%
Critical Value: P=
0.01
PASS: The effluent does not reduce survival of the test organisms.
Reproduction Data
Raw Data
Test for Normality
Mean young/female
Std. Dev.
Test Used: Shapiro -Willis Test:
Control
21.4 1.73
Effluent
21.2 1.85
W:
0.976
Critical Value:
0.884
Analysis for Differences in Reproduction
Test for Homogeneity of Variance
Test Used: Equal Variance t Test.
Test Used: F Test
Calculated t=
0.34
F=
1.14
Critical Value=
2.51
Critical Value=
5.32
The data are homogeneous in variance
PASS: The effluent is not chronically toxic.
Page 13 of 16
Page 3 of 6

Control Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
source
rep
Total
H4 10-21
U310-22
R710-21
K510-21
X510-22
N1 10-21
B310-22
18 10-21
V1010-22
Q510-21
D8 10-22
L310-21
1
3
+8+11
22
2
4
+7+10
21
3
+4+7
11
22
4
3
+6+9
18
5
4
+7+11
22
6
4
+6+10
20
7
4
+7+12
23
8
3
+8+9
20
9
4
+7+10
21
10
3
+7+12
22
11
3
+7+11
21
12
5
+8+12
25
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
0
Mean
20
N/A
0
21.4
H4 10-2
U3 10-2
R7 10-2
K5 10-2
X510-2:
N1 10-2
B3 10-2:
18 10-21
V 10 10-:
Q5 10-2
D810-2
L3 10-2'
0
0
0
0
0
0
0
0
12 % Effluent Mortality and Reproduction by Test Day
1 2 3 4 5 6 7 8
Total
1
4
+8+11
23
2
4
+6+9
19
3
+4+7
10
21
4
+3+8
11
22
5
3
+7+8
18
6
4
+7+11
22
7
3
+8+10
21
8
5
+7+12
24
9
4
+6+10
20
10
3
+7+9
19
11
4
+7+11
22
12
4
+8+11
23
13
N/A
0
14
N/A
0
15
N/A
0
16
N/A
0
17
N/A
0
18
N/A
0
19
N/A
0
Mean
20
N/A
0
212
renew 1
JZ
JC
End Date
fed
JC
JZ
JC
AM
JC
JZ
I 09-Nov-21
time fed & renew
07:46 AM
03:55 PM
11:41 AM
06:41 AM
09:27 AM
11:51 AM
10 15 Ah1
JC
ew temp. °C
24.7
24.5
Old temp. °C
24.8
25.2
25.1
Control New temp. -C
24.5
24.5
Lab#
T60518 C dubia
Client
MSD
Sample ID
WWTP
NPDES#
NC0024911
County
Buncombe
Month
11
Start & fed Date
02-Nov-21
Start & fed Time
03:15 PM
Started & fed By
JC
Test Organism
Ceriodaphnia dubia
Neo. born date
01-Nov-21
Neo. born time
1700-2200
Test Type
NCCPF
Dilution Water
MHSF 10/27/21B
Units for Conc.
IWC
12
%3rd BROOD
Test vessels
30 ml
Test volume
15 ml
Incubator #
1
Light
161t/8dk
Initial Temp °C
24.8
Selenastrum
0.05 m1
YAT
0.05 m1
Test method
EPA 821-R-02-013:1002
Comments
Control ini temp
24.7
60517 AND 60518 SHARE A CONTROL
D=Dead N/A -Lost or not used
Page 4 of 6
Page 14 of 16

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