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HomeMy WebLinkAbout20070812 Ver 1_15 Mar 2005 Tailwater Fish & Aquatic Biota Assessment, Draft Report (2)_20080502Yadkin Project FERC No. 2197 YADKIN TAILWATER FISH AND AQUATIC BIOTA ASSESSMENT DRAFT STUD YREPORT MARCH 2005 YADKIN PROJECT FERC No. 2197 YADKIN TAILWATER FISH AND AQUATIC BIOTA ASSESSMENT DRAFT STUDYREPORT Prepared for ALCOA GENERATING COMPANY, INC. YADKIN DIVISION 293 NC 740 Highway Badin, NC 28009-0576 Prepared by NORMANDEAU ASSOCIATES, INC. 25 Nashua Road Bedford, NH 03110 R-19556.001 March 2005 Tailwater Fish & Aquatic Biota Assessment Draft Table of Contents Page SUMMARY ....................................................................................................................................... vii 1.0 INTRODUCTION ....................................................................................................................1 2.0 BACKGROUND ......................................................................................................................1 3.0 STUDY OBJECTIVES ............................................................................................................1 4.0 METHODS ...............................................................................................................................3 4.1 TAILWATER FISH SAMPLING ............................................................................................3 4.2 TAILWATER FISH ANALYSIS .............................................................................................4 4.3 CAROLINA AND ROBUST REDHORSE SEARCHES ..............................................................5 4.4 TAILWATER MACROINVERTEBRATE SAMPLING AND MUSSEL SEARCHES ......................6 5.0 TAILWATER FISH ASSESSMENT RESULTS ..................................................................6 5.1 HIGH ROCK TAILWATER FISHERIES .......................................................................... .......6 5.1.1 High Rock Tailwater Summer Sample Period .............................................. .......8 5.1.2 High Rock Tailwater Fall Sample Period ..................................................... .....13 5.1.3 High Rock Tailwater Spring Sample Period ................................................. .....14 5.1.4 Seasonal Differences in High Rock Tailwater .............................................. .....14 5.2 TUCKERTOWN TAILWATER FISHERIES ...................................................................... .....15 5.2.1 Tuckertown Tailwater Summer Sample Period ............................................ .....15 5.2.2 Tuckertown Tailwater Fall Sample Period ................................................... .....22 5.2.3 Tuckertown Tailwater Spring Sample Period ............................................... .....22 5.2.4 Seasonal Differences in Tuckertown Tailwater ............................................ .....23 5.3 NARROWS TAILWATER FISHERIES ............................................................................ .....24 5.3.1 Narrows Tailwater Summer Sample Period .................................................. .....29 5.3.2 Narrows Tailwater Fall Sample Period ......................................................... .....31 5.3.3 Narrows Tailwater Spring Sample Period .................................................... .....31 5.3.4 Seasonal Differences in Narrows Tailwater .................................................. .....32 5.4 FALLS TAILWATER FISHERIES ................................................................................... .....33 5.4.1 Falls Tailwater Summer Sample Period ........................................................ .....33 5.4.2 Falls Tailwater Fall Sample Period ............................................................... .....39 5.4.3 Falls Tailwater Spring Sample Period .......................................................... .....40 5.4.4 Seasonal Differences in Falls Tailwater ........................................................ .....41 5.5 TAILWATER FISH SUMMARY ..................................................................................... .....41 5.6 FISH SAMPLING DURING Low AND NORMAL DISSOLVED OXYGEN CONDITIONS .... ..... 43 5.7 FISH STRANDING DURING GENERATION ON/OFF CYCLES ........................................ .....46 5.8 ROBUST AND CAROLINA REDHORSE SEARCHES ....................................................... .....46 6.0 TAILWATER MACROINVERTEBRATE AND MUSSEL ASSESSMENT RESULTS ...............................................................................................................................48 6.1 TRANSECT AND STATION LOCATIONS.. 6.2 TAILWATER DESCRIPTIONS .................. 6.2.1 High Rock Tailwater ................ ....................................................................48 ....................................................................48 ....................................................................48 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 11 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft 6.2.2 Tuckertown Tailwater ........................................................................................54 6.2.3 Narrows Tailwater .............................................................................................54 6.2.4 Falls Tailwater ...................................................................................................54 6.3 MUSSEL SEARCHES ........................................................................................................54 6.4 MACROINVERTEBRATES .................................................................................................56 7.0 IMPACTS OF EXISTING PROJECT OPERATIONS ON FISH AND AQUATIC BIOTA IN THE TAILWATERS ......................................................................62 8.0 CITED REFERENCES .........................................................................................................64 APPENDIX 1: Tailwater Fish and Aquatic Biota Assessment Final Study Plan APPENDIX 2: Length Frequency Distributions for Selected Species from High Rock, Tuckertown, Narrows, and Falls Tailwaters. APPENDIX 3: Narrows Tailwater Long Term Monitor and Plant Generation Data for 2 24- Hour Time Periods Investigating Fish Abundances and Diversity During Periods of Normal and Low Dissolved Oxygen Levels APPENDIX 4: Benthic Macroinvertebrate Survey, Yadkin River, September 2003-June 2004 for Normandeau Associates, Inc. by Pennington and Associates, Inc. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 M Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft List of Figures Page Figure 5-1. High Rock Tailwater Fisheries Sampling Locations ........................................................... 7 Figure 5-2. Tuckertown Tailwater Fisheries Sampling Locations ........................................................16 Figure 5-3. Narrows Tailwater Fisheries Sampling Locations ......................................................... .... 25 Figure 5-4. Falls Tailwater Fisheries Sampling Locations ............................................................... .... 34 Figure 5-5. Dissolved oxygen (mg/L) and total outflow (cfs) for 24 hour period during which electrofish sampling in Narrows tailwater (summer 2003). Shaded area denotes 24 hour period of fish sampling ...................................................................................... .... 44 Figure 5-6. Dissolved oxygen (mg/L) and total outflow (cfs) for 24 hour period during electrofish sampling in Narrows tailwater (summer 2003). Shaded area denotes 24 hour period of fish sampling ...................................................................................... .... 44 Figure 5-7. Species and number of fish of each captured during low and normal dissolved oxygen periods during the 24-hour sampling period in the Narrows tailwater during the summer season ............................................................................................... .... 45 Figure 5-8. Species and number of fish each captured during low and normal dissolved oxygen periods during the 24-hour sampling period in the Narrows tailwater during the fall season .......................................................................................................... 47 Figure 6-1. High Rock Macroinvertebrate Stations and Mussel Transects ..........................................49 Figure 6-2. Tuckertown Macroinvertebrate Stations and Mussel Transects ........................................ 50 Figure 6-3. Narrows Macroinvertebrate Stations and Mussel Transects .............................................. 51 Figure 6-4. Falls Macroinvertebrate Stations and Mussel Transects .................................................... 52 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 iv Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft List of Tables Page Table 5-1. Species composition for High Rock Tailwater by season .................................................... 9 Table 5-2. Percent Composition of Fish Species Captured within High Rock Tailwater, all gear types combined ............................................................................................................10 Table 5-3. Electrofishing CPUE (4of fish per hour of shock time) for fish captured in High Rock Tailwater ....................................................................................................................11 Table 5-4. Gillnet CPUE (# of fish per 24 hour set) for fish captured in High Rock Tailwater .............................................................................................................................. 12 Table 5-5. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr)Values for selected species within High Rock Tailwater during all three sampling seasons ........................................................................................................12 Table 5-6. Species composition for Tuckertown Tailwater by season ................................................17 Table 5-7. Percent Composition of Fish Species Captured within Tuckertown Tailwater, all gear types combined ............................................................................................................18 Table 5-8. Electrofishing CPUE (# of fish per hour of shock time) for fish captured in Tuckertown Tailwater .........................................................................................................19 Table 5-9. Gillnet CPUE (# of fish per 24 hour set) for fish captured in Tuckertown Tailwater .............................................................................................................................. 20 Table 5-10. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr)Values for selected species within Tuckertown Tailwater during all three sampling seasons ........................................................................................................21 Table 5-11. Species composition for Narrows Tailwater by season .....................................................26 Table 5-12. Percent Composition of Fish Species Captured within Narrows Tailwater, all gear types combined ............................................................................................................ 27 Table 5-13. Electrofishing CPUE (# of fish per hour of shock time) for fish captured in Narrows Tailwater ...............................................................................................................28 Table 5-14. Gillnet CPUE (# of fish per 24 hour set) for fish captured in Narrows Tailwater . ........... 29 Table 5-15. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr) Values for selected species within Narrows Tailwater during all three sampling seasons ........................................................................................................30 Table 5-16. Species composition for Falls Tailwater by season ........................................................... 35 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 v Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-17. Percent Composition of Fish Species Captured within Falls Tailwater, all gear types combined .......................................................................................................... Table 5-18. Electrofishing CPUE (# of fish per hour of shock time) for fish captured in Falls Tailwater .................................................................................................................... Table 5-19. Gillnet CPUE (# of fish per 24 hour set) for fish captured in Falls Tailwater . ....... Table 5-20. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr) Values for selected species within Falls Tailwater during all three sampling seasons ....................................................................................................... Table 6-1. Transect locations for mussel and macroinvertebrate sampling, Yadkin tailwaters 2003 ........................................................................................................................... Table 6-2. Physical characteristics of mussel/macroinvertebrate sampling transects within the four project tailwaters .......................................................................................... Table 6-3. Summary of Mollusca taken from Yadkin River, 2003-2004 ................................. Table 6-4. Benthic Macroinvertebrates collected from Yadkin River 2003-2004 (No./-2M2) Table 6-5. Percent composition of the dominant benthic macroinvertebrate species by sampling season ......................................................................................................... Table 6-6. EPT Index and Mlsenhoff Biotic Index scores along with associated water quality for Piedmont area of North Carolina ............................................................ 36 37 38 39 53 53 55 57 62 62 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 vi Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft SUMMARY The Tailwater Fish and Aquatic Biota Assessment Report presents the results of several surveys of fish and aquatic biota (e.g. mussels, benthic macroinvertebrates, etc.) in the tailwaters of the four developments that comprise the Yadkin Project: High Rock, Tuckertown, Narrows and Falls. The project tailwaters are generally those areas where moving water is clearly observable when water is being released from the powerhouses and/or dams. These areas are of particular interest to the resource management agencies because they may provide riverme habitat that can support aquatic species that are native to the free-flowing portions of the Yadkin-Pee Dee River. The study was conducted in accordance with the Final Study Plan that was developed in consultation with Fish and Aquatics Issue Advisory Group (IAG). Specific objectives identified in the Final Study Plan included: ¦ Describe tailwater habitats in all four Yadkin development tailwater areas. ¦ Inventory and assess the resident fish community in the Project tailwaters on a seasonal basis to develop baseline data that can be used to detect changes over time. Evaluate the impacts of existing Project operations on the tailwater fish community, such as impacts associated with generation schedules and impacts due to the low dissolved oxygen found in the tailwaters during certain times of year. ¦ Search for RTE mussel species in Project tailwaters ¦ Search for RTE fish species, including the Robust and Carolina Rehorse species in the Project tailwaters during the spring (spawning period) and during the summary and fall fish surveys. Tailwater aquatic surveys were conducted during the spring, summer and fall seasons. To ensure that the greatest number of species were being collected, fish sampling was done using a variety of methods and gear types including electrofishing and gill nets. Fish were sampled in many tailwater locations including both shallow and deep water habitats. Mussels and benthic macroinvertebrates were sampled along transects established in each of the tailwaters. Mussel searches were conducted by divers swimming along the length of each transect line. Divers searched at least two meters (approximately 6.5 ft) upstream and downstream of each transect line. Additional searches were conducted along the shoreline of each tailrace looking for mussel shells and by having divers search in areas identified by agencies as good mussel habitat that were not located along a transect line. Benthic macroinvertebrates were collected along each transect using an air lift. Benthic organisms were preserved in the field and returned to the laboratory for identification and counting. The initial study effort included a detailed survey and description of the aquatic habitat found in each of the tailwaters. This work was accomplished by establishing transects in each tailwater and doing a detailed survey of substrate and other habitat characteristics along the transect. Another primary objective of the tailwater fish surveys was to develop a comprehensive list of fish species utilizing the tailwaters. The complete list of fish species found in each of the development tailwaters is provided in the table below. 19556.001 Tailwater Fisheries Assessment 3/11/05 vii Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Summary of Fish Species Collected in the Four Yadkin Project Tailwaters Common Name Scientific Name High Rock Tailwater Tucker- town Tailwater Narrows Tailwater Falls Tailwater Blueback Herrin Alosa aestivalis x X X X Gizzard Shad Dorosoma ce edianum x X X X Threadfin Shad Dorosoma etenense x X X X Goldfish Carassius auratus x Common Ca C rinus car io x X X Golden Shiner Notemi onus chr soleucas x X X X S ottail Shiner Notro is hudsonius x Satinfin Shiner C rinella analostana x X X X Eastern Silvery Minnow H bo nathus re ius x Quillback Car iodes c rinus x X X Creek Chubsucker Erim zon oblon us x X X Shorthead Redhorse Moxostoma macrolepidotum x X X X Silver Redhorse Moxostoma anisurum x X X X Flathead Catfish Pylodictus olivarus x X X X Blue Catfish Ictalurus furcatus x X X X Channel Catfish Ictalurus puntatus x X X X White Catfish Ameiurus catus x X X X Flat Bullhead Ameiurus platycephalus x X Yellow Bullhead Ameiurus natalis x Snail Bullhead Ameiurus brunneus x White Perch Morone americana x X X X Hybrid Bass (Striped x White) Morone saxatilis x chrysops x X Striped Bass Morone saxatilis x X X X White Bass Morone chrysops x X X X Redbreast Sunfish Lepomis auritus x X X X Green Sunfish Lepomis cyanellus x X X X Pumpkinseed Lepomis gibbosus x X X X Bluegill Lepomis macrochirus x X X X Redear Sunfish Lepomis microlophus x X X X Warmouth Lepomis gulosus x X X X Smallmouth Bass Micropterus dolomieu x Largemouth Bass Micropterus salmoides x X X X White Crappie Pomoxis annularis x X X X Black Crappie Pomoxis nigromaculatus x X X X Tesselated Darter Etheostome olmstedi x X Yellow Perch Perca flavescens x X X X Longnose Gar Lepisosteus osseus x X X X Smallmouth Buffalo ktiobus bubalus x X X Bowfin Amia calva x White Sucker Catostomus commersoni x Spotted Sucker Minytrema melanops x Overall, the fish communities sampled in the tailwaters of High Rock, Tuckertown, Narrows and Falls developments were found to be very similar, but some differences in species captured were noted. Species diversity recorded in the tailwaters ranged from a high of thirty-four species in both High Rock 19556.001 Tailwater Fisheries Assessment 3/11/05 Vlll Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft and Falls tailwaters to a low of 29 species recorded in Tuckertown tailwater. Large numbers of bluegill, largemouth bass, gizzard shad and white perch dominated the catches in each tailwater. These four species are among the ten most abundant species captured within each tailwater, comprising 48% of the total catch in High Rock tailwater, 57% in Tuckertown tailwater, 64% in Narrows tailwater and 46% in Falls tailwater. These species are generally tolerant of low dissolved oxygen (DO) concentrations, a condition which can occur in the project tailwaters during the summer. Given the numbers of these species captured it also is apparent that these species are well adapted to hydro peaking operations, and routine changes in powerhouse discharges. Another popular sport fish, black crappies, were more abundant in both Tuckertown and High Rock tailwaters than either Narrows or Falls. Common carp and quillback were both in the ten most abundant species sampled in the High Rock tailwater and were either not present or captured in low numbers in the other three tailwaters. The numbers of carp captured in High Rock tailwater were evenly distributed during all three seasons of sampling. Quillback were most abundant in the tailwater during the spring season and may have been using the tailwater area below High Rock dam for spawning. In the Falls tailwater, silver and shorthead redhorse were in the top ten species collected. The shorthead redhorse was captured at all four tailwaters during the study, but its numbers were lower at the other three tailwaters. The higher catches of shorthead redhorse in the Falls tailwater compared to upstream tailwaters may be due to better habitat and water quality conditions, especially dissolved oxygen levels. The shorthead redhorse (and the black redhorse) are considered to be intolerant to poor water quality, as are some darter species (Scott 1999). In terms of the health of the tailwater fisheries, the relative weight values for bluegill and largemouth bass were either within or near the ideal ranges for these species in each of the four tailwaters. Average proportional stock density (PSD) and relative stock density (RSD-P) values for largemouth bass were greater then the ideal range within each of the four tailwaters. Bluegill PSD values were within (High Rock and Narrows) or close to (Tuckertown and Falls) the ideal range for the species in all four tailwaters, suggesting a balanced population. However, RSD-P values for bluegill were well below the ideal range for the species in all four tailwaters and this indicated that few large, quality sized fish were available for harvest. Relative weights for black crappie were within or very close to the ideal range in both Narrows and Tuckertown tailwaters, indicating that the fish are in good condition. However, black crappie relative weights in High Rock tailwater (Tuckertown Reservoir) were lower than both the ideal range, suggesting possible problems finding adequate food sources. The PSD and RSD-P values for black crappie were either within or greater then the ideal range for the species in High Rock, Tuckertown, and Narrows tailwaters, suggesting a balanced population with most size classes represented. Striped bass are currently present within all of the reservoirs and tailwaters, but the numbers captured in the High Rock tailwater (n=11) and Falls tailwater (n=18), were low compared to the numbers capture in the Tuckertown (n=65) and Narrows (n=39) tailwaters. The North Carolina Wildlife Resources Commission (NCWRC) stocks striped bass in all the project reservoirs except Falls (Narrows tailwater). Striped bass captured in the Narrows tailwater (upper Falls reservoir) most likely have dropped down from Narrows Reservoir. Those collected in Falls tailwater (upper Tillery Reservoir) may have originated from stockings into Tillery Reservoir or may have dropped down from Falls Reservoir. Striped bass are known to be relatively sensitive to water temperature and DO conditions, and striped bass in Narrows Reservoir (Tuckertown tailwater) are currently the target of cooperative bioenergetic studies by NCWRC and North Carolina State University to evaluate growth in relation to available habitat, particularly the thermal environment. 19556.001 Tailwater Fisheries Assessment 3/11/05 lx Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Two federal fish species of concern, the Carolina redhorse and robust redhorse, were of particular interest to the fishery agencies during this study. Both species have been collected previously in the Pee-Dee River below the Blewett Falls project, and Carolina redhorse individuals have been collected below Tillery dam and in Tillery Reservoir. For the Yadkin Project study, focused searches for these two species were made in all four tailwaters, with sampling concentrated on Falls tailwater at the upper end of Lake Tillery. Despite the intensive surveys, neither the Carolina redhorse nor the robust redhorse was found in any of the Yadkin Project tailwaters. The study also survey mussels in the four Project tailwaters. A total of seven species of freshwater mussels were found within the four tailwaters. A summary of the mussel species found within each of the four Project tailwaters is provided in the table below. Mussel Species Found in the Yadkin Project Tailwaters Species Falls Dam Tailwater Badin Dam (Narrows) Tailwater Tuckertown Tailwater High Rock Tailwater Anodonta im licata R 1 Alewife floater Elli do com lanta 328 16 Eastern Ellido Elli do c f. lanceolata 113 1 Pee Dee Lance Lampsilis radiata 117 R Eastern lamp mussel Pyganodon cataracte 1 2 Eastern floater Utterbackia imbecillis 8 2 4 1 Paper pond shell Villosa delumbis 8 Eastern creekshell Total No. Of Unionidae Species 7 6 1 1 Total No. Of Individuals 575 22 4 1 Corbicula fluminea A A A A Cipangopalucdinea chinensis 231 Chinese mystery snail • R = represented by relics only • A = abundant Falls tailwater had the greatest mussel diversity with seven species and 575 total individuals. In Falls tailwater, Elliptio complanta (Eastern Elliptio) was the most abundant (57%) mussel species, while Elliptio cf lanceolata (Pee Dee Lance)(20%) and Lampsilis radiata (Eastern lamp mussel)(20%) were common. Narrows tailwater had 6 species with 22 total individuals. Elliptio complanta (73%) was the most abundant species within the Narrows tailwater. Elliptio complanata was present in both the Falls and Narrows tailwaters. One specimen ofAnodonta implicata (Alewife floater) was found within the Narrows tailwater. The only mussel species found in the Tuckertown and High Rock tailwaters was the Utterbackia imbecillis (Paper pond shell) with four individuals found in Tuckertown and one in High Rock. Corbicula fluminea, the Asiatic clam, is an invasive species that was abundant throughout all four tailwaters. There were no federally listed mussel species found within any of the four tailwaters. Elliptio 19556.001 Tailwater Fisheries Assessment 3/11/05 x Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft cf lancolata (PeeDee Lance) is listed as endangered by the state of North Carolina and was found in the tailwaters of both Falls and Narrows. Two species, Anodonta implicata (alewife floater) and Lampsilis radiata (Eastern lamp mussel), are both listed as threatened by the state of North Carolina. Anodonta implicata was found in both Falls (relic shells only) and Narrows tailwaters. Lampsilis radiata was found in Falls and Narrows (relics only) tailwaters. Villosa delumbis (Eastern creekshell) is considered significantly rare by the North Carolina Natural Heritage Program and 8 individuals were found within the Falls tailwater. The study also examined benthic macroinvertebrate communities in each of the tailwaters. Because of their limited mobility, benthic macroinvertebrates are often used as indicators of water quality and aquatic habitat quality. Generally speaking, a more diverse benthic community is indicative of better water quality. At the Yadkin Project, 6 phyla, 24 orders, and 41 families represented by the 99 benthic macroinvertebrates species were found in the four Project tailwaters. Spring sampling in Falls tailwater yielded the highest number of species with 53 found and the summer sampling in High Rock yielded the lowest number of species collected with 29. The spring sampling in Narrows (12,008/12m2) and Falls (10,172/12M2) yielded the highest densities of individuals. The lowest numbers of individuals per sample were recorded in Falls (1,420/12m2) and Narrows (1,333/12m2) during the fall sampling. The table below summarizes the percent composition of the most abundant benthic macroinvertebrate species within each of the four tailwaters during the three seasons of sampling. Percent Composition of the Dominant Benthic Macroinverteb rate Species by Sampling Season in the Yadkin Project Tailwaters September 2003 November 2003 June 2004 Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High SPECIES Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Dugesia tigrina 9.3 7.6 6.2 12.5 Corbicula fluminea 26.7 48.0 43.5 11.0 9.3 Musculium transversum 15.1 9.6 38.2 43.7 6.2 53.2 28.3 18.6 35.2 Physella sp. 8.2 Menetus dilatatus 6.3 Dero sp. 14.1 Slavina appendiculata 14.6 9.1 Lumbriculidae 10.0 Caecidotea sp. 11.1 17.0 10.0 12.6 6.8 17.9 13.8 28.8 17.3 16.3 29.7 6.8 Hyalella azteca 11.1 8.2 6.8 Cyrnellus fraternus 7.3 Cricotopus sp. 15.0 Dicrotendipes simpsoni 24.5 22.3 11.7 Glyptotendipes sp. 9.2 9.2 Rheotanytarsus sp. 22.5 The EPT index is the total number of species found in an area within the pollution sensitive groups Ephemeroptera, Plecoptera, and Trichoptera and is considered a measure of water quality. As the EPT value increases, water quality will tend to do the same. The EPT values computed for all four of the Yadkin Project tailwaters, in all seasons, fell within the fair to poor water quality range. 19556.001 Tailwater Fisheries Assessment 3/11/05 xi Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft 1.0 INTRODUCTION Alcoa Power Generating Inc. (APGI) is applying to the Federal Energy Regulatory Commission for a new license for the Yadkin Hydroelectric Project. The Project consists of four reservoirs (High Rock, Tuckertown, Narrows, and Falls), dams, and powerhouses located on a 38-mile stretch of the Yadkin River in central North Carolina (Figure 1-1). The Project generates electricity to support the power needs of Alcoa's Badin Works and its other aluminum operations, or is sold on the open market. To address concerns over potential impacts of Project operations on aquatic biota in the tailwater reaches of the four developments, a comprehensive, seasonal survey of fish and macroinvertebrates (aquatic insects and mussels) was undertaken in each of the four Project tailwaters. These surveys also included searches for rare, threatened and endangered (RTE) fish and mussel species. 2.0 BACKGROUND As part of the relicensing process, APGI prepared and distributed, in September 2002, an Initial Consultation Document (ICD), which provided a general overview of the Project (APGI 2002). Agencies, municipalities, non-governmental organizations and members of the public were given an opportunity to review the ICD and identify information and studies that were needed to address relicensing issues. To further assist in the identification of issues and data/study needs, APGI formed several Issue Advisory Groups (IAGs) to advise APGI on resource issues throughout the relicensing process. Through meetings, reviews and comments, the Fish and Aquatics IAG assisted in developing the Study Plans for the various resource issues, and will further review and comment on the findings resulting from the implementation of the study plans. The Fish and Aquatics IAG was interested in the effects of Yadkin reservoir releases on tailwater fish, macroinvertebrates and aquatic habitat. They were also interested in the current status of RTE aquatic species in the Project tailwaters that could be impacted by Project operations. This report presents the findings of the tailwater fish and aquatic biota studies, following implementation of the Final Study Plan, dated June 2003. The Final Study Plan, entitled Tailwater Fish and Aquatic Biota Assessment is attached to this report as Appendix 1. 3.0 STUDY OBJECTIVES On March 12 and April 9, 2003 the Fish and Aquatics IAG met to discuss study objectives for the Tailwater Fish and Aquatic Biota study. Through those discussions and written comments on the draft study plan received after the April 9, 2003 IAG meeting, the following objectives were identified for the final study plan. 1. Describe tailwater habitats in all four Yadkin development tailwater areas. Inventory and assess the resident fish community in the Project tailwaters on a seasonal basis (spring, summer, and fall) to develop baseline data that can be used to detect changes over time. Evaluate the impacts of existing Project operations on the tailwater fish community, such as impacts associated with generation schedules (generation on/off), and impacts due to the low dissolved oxygen (DO) found in the tailwaters during certain times of the year. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 1 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft -i- 17 1r ?v ?i u? a ? ucau n?u ??a,e a Environmental Consultants 25 Nashua Road Bedford NH 03 110-5500 (603) 472-5191 0 5 10 Miles I I Figure 1-1. Yadkin Project 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 2 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Inventory and assess the macroinvertebrate and mussel species in the Project tailwaters on a seasonal basis to develop baseline data that can be used to detect changes in these communities over time. Evaluate the impacts of existing project operations on the tailwater macroinvertebrate community and describe tailwater habitats. 4. Search for rare, threatened and endangered mussel species in Project tailwaters. Search for rare, threatened and endangered fish species, including the Robust and Carolina redhorse species, in the Project tailwaters during the spring (spawning period), summer, and fall fish surveys. 4.0 METHODS 4.1 TAILWATER FISH SAMPLING Normandeau personnel intensively sampled for fish during the summer (August and September, 2003), fall (November 2003) and spring (May 2004) in the Falls, Narrows, Tuckertown, and High Rock tailwater areas. Sampling gear included gill nets, boat electroshocking equipment, backpack electrofishing gear, and a forty foot seine net. Sampling focused on the immediate tailwater areas within each of the four reservoirs. The goal was to capture the maximum number of fish species to develop a comprehensive species list of the resident fish community on a seasonal basis in each tailwater. This list would then become a baseline that could be used to detect changes in the fish community over time. Fisheries sampling in each tailwater was planned to include periods when the projects were generating and during periods of no generation or reduced generation (this depended on the season and river flows). Part of this effort included sampling during low dissolved oxygen (DO) and normal DO levels during each season to determine effects of oxygen on fish catches. To accomplish these objectives, sampling in each tailwater generally continued for three to four consecutive days each season, so that different generation (on/off) and normal and low DO periods were sampled. Two sets of experimental, monofilament gill nets, 100 ft long by 8 ft deep, were used to sample each of the tailwaters. The nets were constructed of four 25 ft panels of 1, 2, 3, and 4-inch stretch mesh. The second set of gillnets were of the same construction with 2, 3, 4, and 5 inch stretch mesh. The numbers of nets fished per tailwater varied based upon suitable conditions for setting the gear. Six nets were fished in the Highrock tailwater, seven in Tuckertown, six in Narrows and eight in Falls. Most gill nets were set to fish 24-hour periods and after they were hauled and the fish removed they were usually reset in the same location. However some gill nets were moved to other locations within the tailwater to avoid catching high numbers of repeat species and to expand the species list. Electrofishing in the four tailwaters was conducted from two 16 ft aluminum boats equipped with a 4500W, 230V gasoline-powered generator. A four-electrode array was mounted on a boom and suspended in the water approximately 2 in in front of the boat; the cathode boom was secured to the bow of the boat. Direct current (DC) discharge was controlled by a Smith-Root Model IV electrofisher set to deliver 672V at 5-6 amps at a frequency of 60 pulses/sec. Current to the electrodes was pulsed by a foot switch operated by a netter at the bow. Both shock boats would work opposite shorelines simultaneously to expedite sampling and to ensure that fish were collected from all areas during both generation and non- generation periods. Most of the electrofishing effort in each tailwater concentrated on the shoreline because the mid-channel areas were too deep to effectively fish with anything but gill nets. Electrofishing 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 3 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft was conducted during both daytime and nighttime to maximize catches and to sample during potential low DO periods in order to assess fish community reaction to the oxygen conditions in each tailwater. Each shore was electrofished multiple times to ensure that most fish species were captured. The Smith- Root systems logged the number of seconds each boat applied electricity to the water and this was used to calculate the number of fish captured per minute or the catch per unit effort (CPUE). A backpack electroshocker was used to sample shallow water areas in each tailwater that could not be accessed with the electrofishing boats. A 40 ft seine was also used in appropriate habitat in each of the four tailwaters. All fish were identified to species, measured to the nearest mm, and representative samples were weighed to the nearest gm. Most of the fish captured with electrofishing gear were released alive after they were processed except for a few individuals retained for further ID and reference specimens. Most of the fish captured with gill nets were dead or in poor condition, however, any live fish was released after it was measured and weighed. 4.2 TAILWATER FISH ANALYSIS Percent composition and catch per unit effort (CPUE)was calculated for all fish. Proportional stock density (PSD), relative stock density (RSD) and relative weight (Wr) values were calculated for selected species. Boat electrofishing CPUE was obtained by dividing the total number of captured individuals within species by the number of hours (obtained from the Smith Root log detailing the seconds of electricity applied to the water) to calculate a value representing the number of fish captured per hour of actual shock time. Gill net CPUE was obtained by dividing the total number of captured individuals within each species by the total number of 24 hour net sets to calculate a value representing the number of fish captured per 24 hour period. Proportional stock densities (PSD) and relative stock densities (RSD-P) were calculated for selected game and forage fish. As described by Anderson (1980), PSD is the percentage of the stock that is of quality size and is calculated by: PSD(%) = Number > quality size x 100 Number > stock size where: stock size = 25 percent of maximum length quality size = 37 percent of maximum length RSD is the percentage of the stock that fits one of several size categories; preferred (RSD-P), memorable (RSD-M), or trophy (RSD-T), and is calculated based on the management strategy for the given stock (Gabelhouse 1984). RSD-P(%) = Number > preferred size x 100 Number > stock size where: stock size = 25 percent of maximum length preferred size = 45-55 percent of maximum length In addition to calculating PSD and RSD-P values for the dominant fish species captured in the tailwaters, relative weight was also determined. Relative weight gives an indication of a fish's body condition at the 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 4 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft time of capture. As described by Anderson (1980) and Anderson and Neumann (1996), relative weight (Wr) is an index of body condition and is calculated by: Wr = (W/Ws) x 100 where: W = the weight of an individual WS = a length specific standard weight predicted by a weight-length regression designed to represent a given species Species-specific slope and intercept values are available to fisheries managers to assist in the calculation of relative weight values for a number of sport and non-game fish species (Neumann and Murphy 1991; Muoneke and Pope 1999; Anderson and Neumann 1996; Bister et al. 2000). Standard ranges of PSD and RSD-P values to define a balanced fish population are available to aid fisheries managers. A balanced fish population has been defined as one that is intermediate between large numbers of small fish and small numbers of large fish and it indicates that the rates of growth, recruitment and mortality may be satisfactory (Anderson and Weithman 1978). Largemouth bass PSD values between 40 and 70 and RSD-P values between 10 and 40 are reported as ideal ranges for this species. Bluegill PSD values between 20 and 60 and RSD-P values between 5 and 20 are ideal for this fish. Crappie PSD values between 30 and 60 and RSD-P values between 5 and 20 are indicative of balanced populations (Anderson 1980; Anderson and Newman 1996). In general, an average relative weight of 100 over a broad range of size groups may reflect the optimum ecological and physiological conditions for a given species. Relative weight values well below the optimum range may be indicative of a problem with food or feeding for the species of concern. Ideal ranges of 95 to 100 for largemouth bass, bluegill, and redear sunfish (Murphy et al. 1991) and 95 to 105 for black and white crappie (Neumann and Murphy 1991) have been suggested. Of important note to fisheries managers, it has been suggested that populations of bass with low or no annual reproduction or low or indeterminate mortality of quality-sized fish may have PSD values greater then 80, RSD-P values greater then 40, and relative weight values exceeding 110. Populations of bluegill associated with above described bass populations typically have PSD values less then 20 and RSD-P values around zero (Anderson 1980). 4.3 CAROLINA AND ROBUST REDHORSE SEARCHES The robust redhorse (moxostoma robustum) and Carolina redhorse (undescribed moxostoma species) are both listed as a Federal Species of Concern by the U.S. Fish and Wildlife Service (USFWS) and in recent years small numbers of both species have been captured in the Pee Dee River downstream of the Yadkin Project (RRCC 2003). Field crews were prepared to collect and hold these fish alive during all three seasons of tailwater fish capture. During the spring 2004 tailwater fish sampling, an additional effort was expended at all four tailwaters to try and capture the two redhorse species. Most of the available spawning habitat for the redhorse species at the Yadkin Project exists in the rocky, shoal habitat present in the four tailwaters, therefore the spring fish sampling was expanded to try and capture spawning redhorse species. Spring (2004) fish sampling began in the Falls tailwater when water temperatures reached 18 °C (robust redhorse spawn between 18 and 24°C). Additional gill net sets and electrofishing transects were fished in Falls tailwater during the spring sampling period, mainly because Falls has more of the redhorses preferred riverine habitat compared to the other Project tailwaters. Carolina redhorse have been captured in the Pee Dee River below the Yadkin Project around woody debris, therefore field crews also targeted this habitat type, when present, with both gill nets and electroshocking gear. Gill net locations at 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 5 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft all four tailwaters were moved frequently during the spring sample period in an effort to cover more area. Gill nets were fished and checked within 8-hour periods during the spring survey to avoid any potentially negative effects on the rare redhorse species. 4.4 TAILWATER MACROINVERTEBRATE SAMPLING AND MUSSEL SEARCHES Normandeau and Pennington and Associates, Inc. (PAI) personnel set-up permanent mussel transects and macroinvertebrate sampling stations in each of the four Project tailwaters. Agency personnel and interested participants from the Fish and Aquatics IAG assisted in picking out the transect locations at each tailwater site during the site visits in July 2003. Once the transect locations were selected, a GPS reading was taken on each shoreline so all macroinvertebrate sampling and mussel searches would be taken from the same transects. Two transects were setup in each of the four tailwaters, with one transect located near each powerhouse and the other located downstream in the lower tailwater. Along each transect, three permanent macroinvertebrate stations were established at approximately 25%, 50% and 75% of the distance along each transect. Sampling was conducted at the macroinvertebrate stations during summer (September 2003), fall (November, 2003), and spring (May, 2004). A total of six 2 m2 macroinvertebrate samples were collected from each tailwater per sample period. In deep water (>4 ft), an underwater airlift was used by a diver to collect the 2 m2 macroinvertebrate samples at each station and in shoal water, a kick net was used. Macroinvertebrates collected were preserved on-site and returned to PAI's lab for sorting and identification of species. Mussel searches were also conducted each season in each of the four tailwaters by divers swimming along the length of each transect line (length dependent on the wetted width of each tailwater at time of sampling). Divers searched at least one meter upstream and downstream of each transect line (2 in wide band along the entire transect) when visibility permitted. Additionally, mussel searches were done by walking along the shoreline of each tailwater looking for mussel shells and by having divers search in areas identified by participants during the July 2003 site visit as good mussel habitat that was not located along a transect line. Any live RTE mussel species located during the searches were to be identified, returned to where it was found (if it was removed from the water), and its location recorded with GPS. The location of any relic mussel shells found would be recorded and the shells collected and identified. The benthic macroinvertebrate communities were assessed and compared using taxa richness, percent contribution of dominant taxon, EPT index, Jaccards Coefficient, Percent Similarity and a modified Hilsenhoff Biotic Index. During the macroinvertebrate sampling and mussel searches planned for August/September 2003, Normandeau and PAI biologists collecting the samples described the habitat found in each tailwater area along the transect lines. 5.0 TAILWATER FISH ASSESSMENT RESULTS 5.1 HIGH ROCK TAILWATER FISHERIES This section presents results of all the fish captured in Highrock tailwater during the three sampling periods. Figure 5-1 shows the seasonal gill net locations, the extent of the area that was electrofished by boat, and the locations of backpack electrofishing and beach seining stations. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 6 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 5-1. High Rock Tailwater Fisheries Sampling Locations. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 7 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft A total of 2,275 fish (all gear types and seasons combined) representing 33 species and one hybrid were captured in the High Rock tailwater during the study period (Tables 5-1 & 5-2). Bluegill (15.7%), white perch (14.2%) and channel catfish (10%) were the three most common fish species collected (Table 5-2). A total of 1,171 fish were captured by electrofishing during all three seasons of sampling (Table 5-3). Bluegills were the dominant species electrofished, with a CPUE of 47.7 fish/hr. Gill nets captured a total of 942 fish (Table 5-4). White perch (CPUE = 3.9 fish) were the most common fish captured using this gear. A total of 154 individuals representing five species (golden shiner, largemouth bass, satinfin shiner, spottail shiner and threadfin shad) were captured by beach seine (all seasons combined) and backpack shocking yielded eight individuals representing six species (bluegill, flathead catfish, green sunfish, pumpkinseed, satinfin shiner, and white crappie). 5.1.1 High Rock Tailwater Summer Sample Period A total of 706 fish, representing 25 species were captured in High Rock tailwater (all gear types combined) between September 15 and September 18, 2003 (Table 5-2). Bluegill were the dominant fish species collected (18.8%) followed by white perch (16.0 %), channel catfish (13.0 %) and common carp at 9.4 % (Table 5-2). These four fish accounted for more than 57 % of all the fish captured during the summer period. Electrofishing in High Rock tailwater during the summer sample period resulted in 20 species being captured. Total electrofishing CPUE (all species combined) was 281 fish per hour with a total of 318 individual fish captured (Table 5-3). Bluegills were the most abundant fish species captured with a CPUE of 109.7 fish per hour followed by largemouth bass (42.5 fish per hour), black crappie (32.7 per hour) and gizzard shad (30.1 per hour). Combined, these four species represent 76 % of all the fish captured by electrofishing during the summer period. Total CPUE for fish (all species combined) collected in gill nets deployed in High Rock tailwater during the summer sample period was 11.3 fish per net, and this consisted of 338 fish representing 20 species (Table 5-3). White perch were the dominant gill net fish captured, with a CPUE of 3.3 fish per net followed by channel catfish (2.9 per net) and common carp (1.8 per net). These three fish combined made up 71 % of the summer gill net catches. Beach seining in the High Rock tailwater during the summer sample period yielded 50 total fish, representing three species (44 satinfin shiner, 4 golden shiner, and 2 threadfin shad). No fish were captured during backpack electrofishing. Values for PSD and RSD-P were calculated for largemouth bass, black crappie and bluegill captured in High Rock tailwater during the summer period (Table 5-5). The average PSD and RSD-P values for largemouth bass were 89 and 58, respectively, and both these values exceeded the ideal range reported. This means some of the large quality sized fish are experiencing low mortality and this may be due to catch and release fishing practices. Black crappie had an average PSD of 24, which is lower than the ideal range of 30 to 60 reported for a balanced population, but the RSD-P value of 10 is within the ideal range reported, suggesting that some large fish are available (Table 5-5). A low sample size prevented the calculation of PSD and RSD-P values for white crappie during the summer season. The average bluegill PSD (22) was within the range for a balanced population, but the average RSD-P (2) was below, indicating few large bluegills were present. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 8 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-1. Species composition for High Rock Tailwater by season. Sampling Periods Common Name Scientific Name Summer 2003 Fall 2003 S rin 2004 All Seasons Blueback Herring Alosa aestivalis x X Gizzard Shad Dorosoma cepedianum x X X X Threadfin Shad Dorosoma petenense x X X X Goldfish Carassius auratus x X Common Carp Cyprinus carpio x X X X Golden Shiner Notemigonus chrysoleucas x X X X Spottail Shiner Notropis hudsonius x X Satinfin Shiner Cyprinella analostana x X X X Eastern Silvery Minnow Hybognathus regius x X Quillback Carpiodes cyprinus x X X X Creek Chubsucker Erimyzon oblongus x X Shorthead Redhorse Moxostoma macrolepidotum x X X X Silver Redhorse Moxostoma anisurum x X X White Catfish Ameiurus catus x X Channel Catfish ktalurus puntatus x X X X Flathead Catfish Pylodictus olivarus x X X X White Perch Morone americana x X X X Hybrid Bass (Striped x White) Morone saxatilis x chrysops x X X Striped Bass Morone saxatilis x X X X White Bass Morone chrysops x X Redbreast Sunfish Lepomis auritus x X X X Green Sunfish Lepomis cyanellus x X X X Pumpkinseed Lepomis gibbosus x X X X Bluegill Lepomis macrochirus x X X X Largemouth Bass Micropterus salmoides x X X X White Crappie Pomoxis amrularis x X X X Black Crappie Pomoxis nigromaculatus x X X X Tesselated Darter Etheostome olmstedi x X Yellow Perch Perca flavescens x X X Longnose Gar Lepisosteus osseus x X X X Warmouth Lepomis gulosus x X X Smallmouth Buffalo ktiobus bubalus x X Blue Catfish ktalurus furcatus x X X Redear Sunfish Lepomis microlophus x X X X Total Taxa 25 26 28 34 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 9 Normandeau Associates, inc. Table 5-2. Percent Composition of Fish Species Captured within High Rock Tailwater, all gear types combined. O Summer 2003 Fall 2003 S rin 2004 All Seasons Species # Individuals % Composition # Individuals % Composition # Individuals % Composition # Individuals % Composition Blue ill 133 18.84% 150 19.53% 75 9.36% 358 15.74% White Perch 113 16.01% 152 19.79% 58 7.24% 323 14.20% Channel Catfish 92 13.03% 73 9.51% 63 7.87% 228 10.02% Largemouth Bass 55 7.79% 63 8.20% 89 11.11% 207 9.10% Gizzard Shad 55 7.79% 46 5.99% 103 12.86% 204 8.97% Common Carp 66 9.35% 48 6.25% 51 6.37% 165 7.25% Black Crappie 51 7.22% 34 4.43% 63 7.87% 148 6.51% Golden Shiner 5 0.71% 54 7.03% 66 8.24% 125 5.49% Satinfin Shiner 54 7.65% 27 3.52% 16 2.00% 97 4.26% Quillback 10 1.42% 1 0.13% 68 8.49% 79 3.47% Threadfin Shad 8 1.13% 40 5.21% 19 2.37% 67 2.95% Pumpkinseed 4 0.57% 22 2.86% 14 1.75% 40 1.76% White Crappie 5 0.71% 6 0.78% 27 3.37% 38 1.67% Shorthead Redhorse 4 0.57% 11 1.43% 19 2.37% 34 1.49% Flathead Catfish 10 1.42% 4 0.52% 9 1.12% 23 1.01% Green Sunfish 10 1.42% 8 1.04% 3 0.37% 21 0.92% Yellow Perch 0 0.00% 1 0.13% 16 2.00% 17 0.75% Longnose Gar 3 0.42% 2 0.26% 9 1.12% 14 0.62% Blue Catfish 13 1.84% 0 0.00% 0 0.00% 13 0.57% Silver Redhorse 1 0.14% 11 1.43% 0 0.00% 12 0.53% Striped Bass 3 0.42% 6 0.78% 2 0.25% 11 0.48% White Bass 0 0.00% 0 0.00% 8 1.00% 8 0.35% Redear Sunfish 1 0.14% 2 0.26% 4 0.50% 7 0.31% Redbreast Sunfish 2 0.28% 0 0.00% 4 0.50% 6 0.26% Creek Chubsucker 0 0.00% 0 0.00% 5 0.62% 5 0.22% Warmouth 1 0.14% 1 0.13% 3 0.37% 5 0.22% White Catfish 3 0.42% 2 0.26% 0 0.00% 5 0.22% Goldfish 0 0.00% 0 0.00% 3 0.37% 3 0.13% Hybrid Bass (Striped x White) 1 0.14% 2 0.26% 0 0.00% 3 0.13% Blueback Herring 0 0.00% 2 0.26% 0 0.00% 2 0.09% Smallmouth Buffalo 2 0.28% 0 0.00% 0 0.00% 2 0.09% Tesselated Darter 0 0.00% 0 0.00% 2 0.25% 2 0.09% Eastern Silvery Minnow 0 0.00% 0 0.00% 1 0.12% 1 0.04% Spottail Shiner 0 0.00% 0 0.00% 1 0.12% 1 0.04% White Sucker 1 0.14% 0 0.00% 0 0.00% 1 0.04% TOTALS 706 100.00% 768 100.00% 801 100.00% 2275 100.00% rz) Tailwater Fish & Aquatic Biota Assessment Draft Table 5-3. Electrofishing CPUE (#of fish per hour of shock time) for fish captured in High Rock Tailwater. Sumner 2003 Fall 2003 Spring 2004 All Seasons S ecies # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE Bluegill 124 109.65 146 47.31 72 24.35 342 47.67 Largemouth Bass 48 42.45 60 1944 . 81 27.39 189 26.34 Gizzard Shad 34 30.07 40 12.96 28 9.47 102 14.22 Black Crappie 37 32.72 28 9.07 34 11.50 99 13.66 Common Carp 11 9.73 36 11.67 19 6.42 66 9.20 Threadfin Shad 5 4.42 40 12.96 17 5.75 62 8.64 Satinfin Shiner 10 8.84 25 8.10 14 4.73 49 6.83 Pumpkinseed 4 3.54 21 6.80 14 4.73 39 5.44 Quillback 0 0.00 0 0.00 29 9.81 29 4.04 Golden Shiner 1 0.88 8 2.59 16 5.41 25 3.48 White Perch 14 12.38 2 0.65 5 1.69 21 2.93 Channel Catfish 6 5.31 8 2.59 5 1.69 19 2.65 Green Sunfish 10 8.84 6 1.94 3 1.01 19 2.65 Yellow Perch 0 0.00 1 0.32 16 5.41 17 2.37 Silver Redhorse 1 0.88 11 3.56 0 0.00 12 1.67 White Crappie 4 3.54 4 1.30 4 1.35 12 1.67 Flathead Catfish 1 0.88 3 0.97 7 2.37 11 1.53 Striped Bass 2 1.77 6 1.94 2 0.68 10 1.39 Shorthead Redhorse 2 1.77 2 0.65 4 1.35 8 1.12 Redear Sunfish 1 0.88 2 0.65 4 1.35 7 0.98 Redbreast Sunfish 2 1.77 0 0.00 4 1.35 6 0.84 Longnose Gar 0 0.00 0 0.00 5 1.69 5 0.70 White Bass 0 0.00 0 0.00 5 1.69 5 0.70 Creek Chubsucker 0 0.00 0 0.00 4 1.35 4 0.56 Warmouth 0 0.00 1 0.32 3 1.01 4 0.56 Blueback Herring 0 0.00 2 0.65 0 0.00 2 0.28 Hybrid Bass (Striped x White) 0 0.00 2 0.65 0 0.00 2 0.28 Tesselated Darter 0 0.00 0 0.00 2 0.68 2 0.28 Eastern Silvery Minnow 0 0.00 0 0.00 1 0.34 1 0.14 Goldfish 0 0.00 0 0.00 1 0.34 1 0.14 White Catfish 1 0.88 0 0.00 0 0.00 1 0.14 TOTALS 318 281.2 454 147.09 399 134.91 1171 163.1 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 11 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-4. Gillnet CPUE (# of fish per 24 hour set) for fish captured in High Rock Tailwater. Summer 2003 Fall 2003 S rin 2004 All Seasons S ecies # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE White Perch 99 3.30 150 8.33 53 1.77 302 3.87 Channel Catfish 86 2.87 65 3.61 58 1.93 209 2.68 Gizzard Shad 21 0.70 6 0.33 75 2.50 102 1.31 Common Carp 55 1.83 12 0.67 32 1.07 99 1.27 Quillback 10 0.33 1 0.06 39 1.30 50 0.64 Black Crappie 14 0.47 6 0.33 29 0.97 49 0.63 Shorthead Redhorse 2 0.07 9 0.50 15 0.50 26 0.33 White Crappie 1 0.03 1 0.06 23 0.77 25 0.32 Bluegill 9 0.30 2 0.11 3 0.10 14 0.18 Largemouth Bass 7 0.23 1 0.06 6 0.20 14 0.18 Blue Catfish 13 0.43 0 0.00 0 0 13 0.17 Flathead Catfish 9 0.30 1 0.06 1 0.03 11 0.14 Longnose Gar 3 0.10 2 0.11 4 0.13 9 0.12 White Catfish 2 0.07 2 0.11 0 0.00 4 0.05 Threadfin Shad 1 0.03 0 0.00 2 0.07 3 0.04 White Bass 0 0.00 0 0.00 3 0.10 3 0.04 Goldfish 0 0.00 0 0.00 2 0.07 2 0.03 Smallmouth Buffalo 2 0.07 0 0.00 0 0.00 2 0.03 Creek Chubsucker 0 0.00 0 0.00 1 0.03 1 0.01 Hybrid Bass (Striped x White) 1 0.03 0 0.00 0 0.00 1 0.01 Striped Bass 1 0.03 0 0.00 0 0.00 1 0.01 Warmouth 1 0.03 0 0.00 0 0.00 1 0.01 White Sucker 1 0.03 0 0.00 0 0.00 1 0.01 TOTALS 338 11.25 258 14.34 346 11.54 942 12.08 Table 5-5. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr)Values for selected species within High Rock Tailwater during all three sampling seasons. PSD S ecies Summer Fall S r?n All Seasons Ideal Range Black crappie 24 42 24 27 30 to 60* Bluegill 22 40 25 26 20 to 60* Largemouth bass 89 83 87 87 40 to 70* RSD-P Species Summer Fall S r?n All Seasons Ideal Range Black crappie 10 9 8 8 5 to 20* Bluegill 2 0 1 1 5 to 20* Largemouth bass 58 69 55 58 10 to 40* Wr S ecies Summer Fall S r?n All Seasons Ideal Range Black crappie 82 80 80 80 95 to 105** Bluegill 95 98 94 95 95 to 100*** Channel catfish 87 88 90 89 90 to 100* Largemouth bass 104 110 104 102 95 to 100*** [*(Anderson and Neumann 1996) **(Neumann and Murray 1991) ***(Murphy et al. 1991) 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 12 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-5 shows the average relative weights (Wr) for four fish species captured in High Rock tailwater by season. Black crappie had an average relative weight of 82 during the summer sampling season, which is lower than the recommended values of 95 to 105 reported for that species, indicating a problem securing enough food. The average bluegill relative weight for the summer sampling period was 95 and this was within the acceptable range (95-100) for the species. Average largemouth bass relative weights (104) for the summer period were slightly higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 87) were slightly below the recommended range of 90 to 100 reported for the species. 5.1.2 High Rock Tailwater Fall Sample Period A total of 768 fish, representing 26 species were captured in High Rock tailwater (all gear types combined) between November 6 and November 8, 2003 (Table 5-1). White perch (19.8 %) and bluegill (19.5 %) were the two most abundant species during the fall sampling followed by channel catfish (9.5 %) and largemouth bass (8.2 %) (Table 5-2). Together, these four fish species comprised 57 % of all the fish captured. Golden shiners were the fifth most abundant fish within the tailwater area during the fall and the majority of these were captured by beach seine in shallow, low-flow areas with sandy substrate. Electrofishing CPUE in High Rock tailwater averaged 147 fish per hour during the fall sampling period and from this effort, 454 fish, representing 22 species were captured by electrofishing (Table 5-3). Bluegills were the dominant fish captured electrofishing, with a CPUE of 47.3 fish per hour, followed by largemouth bass (19.4 per hour), gizzard shad (13.0 per hour), threadfin shad (13.0 per hour) and common carp (11.7 per hour). Gillnet CPUE for the fall sampling, all species combined, averaged 14.3 fish per net (Table 5-4). Two hundred fifty-eight fish representing 13 species were captured in the gill nets. White perch dominated the gill net catch with a CPUE of 8.3 fish per net, comprising 58% of the total fish caught. During the fall sampling, a total of 50 fish representing three species were captured by beach seining (46 golden shiner, 2 satinfin shiner, 2 largemouth bass). An additional six fish representing 4 species were captured by backpack electrofishing (2 green sunfish, 2 bluegills, 1 pumpkinseed and 1 white crappie). Values for PSD and RSD-P were calculated for largemouth bass, black crappie and bluegills collected during the fall sample period (Table 5-5). The average PSD and RSD-P for largemouth bass was 83 and 69, respectively, and both these values exceeded the ideal range reported. This indicates some of the large quality sized bass are experiencing low mortality and this may be due to catch and release fishing practices. Black crappie had an average PSD of 42 and RSD-P of 9, suggesting a balanced population with some large fish available for harvest. A low sample size prevented the calculation of PSD/RSD-P values for white crappie during the fall season. The average bluegill PSD (40) was within the range for a balanced population, while the average RSD-P was zero, indicating no preferred size fish. Table 5-5 shows the average relative weights for four fish species captured in High Rock tailwater by season. Black crappie had an average relative weight of 80 during the fall sampling season, which is lower than the recommended values of 95 to 105 reported for that species. This indicates that the black crappies may have a problem securing enough food. The average bluegill relative weight for the fall sampling period was 98 and is within the acceptable range (95-100) for the species. Average largemouth bass relative weights (110) for the fall period were slightly higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 88) were just below the recommended range for the species. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 13 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft 5.1.3 High Rock Tailwater Spring Sample Period A total of 801 fish, representing 28 species were captured in High Rock tailwater (all gear types combined) between May 4 and May 6, 2003 (Tables 5-1). Gizzard shad (12.9%), largemouth bass (11.1%), and bluegill (9.4%) were the three most abundant species captured in the tailwater during spring sampling (Table 5-2). Quillback were the fourth most abundant species captured, making up 8.5% of the fish caught, and field notes suggest the gear was not completely effective at capturing quilback. Large numbers of quillback were observed escaping capture by moving out of the field generated by the electrofishing boats. Golden shiners were the fifth most abundant species collected during the spring season, comprising 8.3% of the total catch. The majority of these fish were captured by beach seine in shallow, sandy, low-flow areas of the tailwater. Spring electrofishing CPUE averaged 135 fish per hour, with a total of 399 fish (27 species) captured (Table 5-3). Largemouth bass (CPUE=27.4 per hour) and bluegill (24.4 per hour) were the dominant species and comprised over 38% of all the fish caught. Black crappie (11.5 per hour), quillback (9.8 per hour) and gizzard shad (9.5 per hour) were also regularly captured by electrofishing. Total CPUE for gill nets fished during the spring season averaged 11.5 fish per net, per 24-hour set (Table 5-4). A total of 346 fish representing 16 species were captured, and of these, gizzard shad (2.5 per net), channel catfish (1.9 per net), white perch (1.8 per net) and quillback (1.3 per net) were the dominant species. During the spring sampling, a total of 54 fish representing four species were captured by beach seining (50 golden shiners, 2 largemouth bass, 1 satinfin shiner and 1 spottail shiner). An additional two fish (1 satinfin shiner and 1 flathead catfish) were captured by backpack electrofishing The average PSD and RSD-P values for largemouth bass were 87 and 55, respectively, and both these values exceeded the ideal range reported (Table 5-5). This indicates that some of the large quality sized fish are experiencing low mortality and this may be due to catch and release fishing practices. Black crappie had an average PSD of 24, which is lower than the ideal range of 30 to 60 reported for a balanced population, but the RSD-P value of 8 is within the ideal range reported, suggesting that some large fish are available. Table 5-5 shows the average relative weights for four fish species captured in High Rock tailwater by season. Black crappie had an average relative weight of 80 during the spring sampling season, which is lower than the recommended values of 95 to 105 reported for that species. Relative weights for black crappie were low during all three seasons in High Rock tailwater, suggesting a problem securing enough food. The average bluegill relative weight for the spring sampling period was 94, near the acceptable range (95-100) for the species. Average largemouth bass relative weights (104) for the spring period were slightly higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 90) were within the recommended range for the species. 5.1.4 Seasonal Differences in High Rock Tailwater Species abundance was highest in High Rock tailwater during the spring sampling period. Bluegill, largemouth bass, channel catfish and gizzard shad were among the top seven species collected in each of the three sampling periods. Quillback abundance showed a dramatic increase during the spring sampling period when compared to the summer and fall periods. Quillback in Tennessee have been shown to spawn from late April through May (Etmer and Starnes 1993). Quillback may be using the area below 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 14 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft the dam as a spawning ground. The PSD and RSD-P values for black crappie, largemouth bass, and bluegill followed the same pattern through all three sampling events. Using criteria from Anderson (1980), the High Rock PSD, RSD-P and Wr values for largemouth bass from all seasons combined were suggestive of a population with low mortality among quality-sized fish. The relative weights calculated for black crappie were consistently below ideal ranges, suggesting a feeding problem. Bluegill and channel catfish relative weights were either in the ideal range or just below, indicating these fish are in good condition. Largemouth bass consistently exceeded Wr values, indicating these fish are in very good condition, with no problems securing food. Appendix 2 (Figures 1-1 through 1-9) show the length frequency distributions for several important game and forage fish found in High Rock tailwater during all three sampling seasons. Largemouth bass are spawning successfully within High Rock tailwater or the upper reach of Tuckertown reservoir. The length-frequency figures for this species show a bimodal distribution with peaks for juvenile and adult sized fish. Small numbers of striped bass were present in the tailwater during all three sampling periods. Striped bass are stocked by NCWCR to enhance the sport fishery and as a management tool to consume shad. Channel catfish ranging in size from <5cm to >60 cm were present in the tailwater during all three seasons. Channel catfish are maintaining a population with some large-sized individuals within the tailwater and are a popular sport fish. Bluegills are spawning successfully with a large number of individuals in the 12-15 cm size-classes, but there are few large quality fish. Black crappies were captured in all three seasons in the tailwater and those individuals represented a wide range of size classes, from juvenile to adult fish. White crappies were less abundant but individuals from both adult and juvenile size classes were captured. Large numbers of forage species such as white perch, gizzard shad, and threadfin shad indicate these fish are spawning successfully 5.2 TUCKERTOWN TAILWATER FISHERIES This section presents results of all the fish captured in the Tuckertown tailwater during the three sampling periods. Figure 5-2 shows the seasonal gill net locations, the extent of the area that was electrofished by boat, and the locations of backpack electrofishing and beach seining stations. A total of 3,296 fish (all gear types and seasons combined) representing 28 species and one hybrid were captured in the Tuckertown tailwater during the study (Tables 5-6 & 5-7). Bluegill (18.2%), gizzard shad (18.1) and white perch (13.7%) were the top three species captured (Table 5-7). A total of 1,836 fish were captured by electrofishing during all three seasons of sampling, and of these, bluegill were the most commonly captured species with a CPUE of 48.2 fish captured per hour of electroshocking (Table 5-8). A total of 1,438 fish were captured by gill nets during the three seasons of sampling. White perch were the most common species with a CPUE of 4.3 per net (Table 5-9). A total of 13 fish representing two species (bluegill and largemouth bass) were captured by beach seine during all three sampling seasons. Backpack shocking yielded nine individuals representing five species (bluegill, channel catfish, flathead catfish, golden shiner, largemouth bass). 5.2.1 Tuckertown Tailwater Summer Sample Period A total of 1,423 fish, representing 24 species were captured in the Tuckertown tailwater (all gear types combined) between September 1 and September 4, 2003 (Tables 5-7). Gizzard shad (29.9%), bluegill 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 15 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 5-2. Tuckertown Tailwater Fisheries Sampling Locations. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 16 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-6. Species composition for Tuckertown Tailwater by season. Sampling Periods Common Name Scientific Name Summer 2003 Fall 2003 S rin 2004 All Seasons Blueback Herring Alosa aestivalis x X X X Gizzard Shad Dorosoma cepedianum x X X X Threadfin Shad Dorosoma petenense x X X X Common Carp Cyprinus carpio x X X X Golden Shiner Notemigonus chrysoleucas x X X Satinfin Shiner Cyprinella analostana x X X Quillback Carpiodes cyprinus x X X Creek Chubsucker Erimyzon oblongus x X Shorthead Redhorse Moxostoma macrolepidotum x X X X Silver Redhorse Moxostoma anisurum x X X White Catfish Ameiurus catus x X X X Channel Catfish ktalurus puntatus x X X X Flathead Catfish Pylodictus olivarus x X X X White Perch Morone americana x X X X Hybrid Bass (Striped x White) Morone saxatilis x chrysops x X X Striped Bass Morone saxatilis x X X X White Bass Morone chrysops x X Redbreast Sunfish Lepomis auritus x X X X Green Sunfish Lepomis cyanellus x X X X Pumpkinseed Lepomisgibbosus x X X X Bluegill Lepomis macrochirus x X X X Largemouth Bass Micropterus salmoides x X X X White Crappie Pomoxis amrularis x X X X Black Crappie Pomoxis nigromaculatus x X X X Yellow Perch Perca flavescens x X X X Longnose Gar Lepisosteus osseus x X X X Warmouth Lepomis gulosus x X Blue Catfish ktalurus furcatus x X X Redear Sunfish Lepomis microlophus x X X X Total Taxa 24 26 25 29 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 17 Normandeau Associates, inc. Table 5-7. Percent Composition of Fish Species Captured within Tuckertown Tailwater, all gear types combined. -777 Summer 2003 Fall 2003 S rin 2004 All Seasons Species # Individuals % Composition # Individuals % Composition # Individuals % Composition # Individuals % Composition Bluegill 267 18.76% 244 20.75% 88 12.63% 599 18.17% Gizzard Shad 426 29.94% 116 9.86% 53 7.60% 595 18.05% White Perch 224 15.74% 164 13.95% 63 9.04% 451 13.68% Channel Catfish 100 7.03% 141 11.99% 73 10.47% 314 9.53% Largemouth Bass 78 5.48% 79 6.72% 79 11.33% 236 7.16% Threadfin Shad 53 3.72% 76 6.46% 69 9.90% 198 6.01% Black Crappie 37 2.60% 83 7.06% 27 3.87% 147 4.46% White Crappie 43 3.02% 29 2.47% 20 2.87% 92 2.79% Pumpkinseed 5 0.35% 9 0.77% 62 8.90% 76 2.31% Flathead Catfish 26 1.83% 4 0.34% 35 5.02% 65 1.97% Striped Bass 15 1.05% 43 3.66% 7 1.00% 65 1.97% Yellow Perch 2 0.14% 21 1.79% 37 5.31% 60 1.82% White Catfish 34 2.39% 6 0.51% 18 2.58% 58 1.76% Redear Sunfish 19 1.34% 31 2.64% 6 0.86% 56 1.70% Blueback Herring 41 2.88% 10 0.85% 4 0.57% 55 1.67% Shorthead Redhorse 15 1.05% 38 3.23% 1 0.14% 54 1.64% Common Carp 16 1.12% 16 1.36% 21 3.01% 53 1.61% Hybrid Bass (Striped x White) 2 0.14% 20 1.70% 0 0.00% 22 0.67% Golden Shiner 0 0.00% 18 1.53% 3 0.43% 21 0.64% Blue Catfish 8 0.56% 1 0.09% 8 1.15% 17 0.52% Quillback 1 0.07% 7 0.60% 7 1.00% 15 0.46% Silver Redhorse 2 0.14% 9 0.77% 0 0.00% 11 0.33% Longnose Gar 3 0.21% 1 0.09% 5 0.72% 9 0.27% Satinfin Shiner 0 0.00% 7 0.60% 1 0.14% 8 0.24% Redbreast Sunfish 2 0.14% 1 0.09% 3 0.43% 6 0.18% White Bass 0 0.00% 0 0.00% 5 0.72% 5 0.15% Green Sunfish 1 0.07% 1 0.09% 2 0.29% 4 0.12% Warmouth 3 0.21% 0 0.00% 0 0.00% 3 0.09% Creek Chubsucker 0 0.00% 1 0.09% 0 0.00% 1 0.03% TOTALS 1423 100.00% 1176 100.00% 697 100.00% 3296 100.00% rz) Tailwater Fish & Aquatic Biota Assessment Draft Table 5-8. Electrofishing CPUE (# of fish per hour of shock time) for fish captured in Tuckertown Tailwater. Summer 2003 Fall 2003 Spring 2004 7 77A711 Seasons Species # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE Bluegill 256 58.42 233 74.44 73 17.60 562 48.20 Gizzard Shad 283 64.58 47 15.02 53 12.78 383 32.85 Largemouth Bass 66 15.06 77 24.60 73 17.60 216 18.52 Threadfin Shad 42 9.58 56 17.89 19 4.58 117 10.03 Pumpkinseed 5 1.14 9 2.88 62 14.95 76 6.52 White Perch 32 7.30 21 6.71 5 1.21 58 4.97 Black Crappie 16 3.65 26 8.31 7 1.69 49 4.20 Redear Sunfish 11 2.51 30 9.58 6 1.45 47 4.03 Blueback Herring 41 9.36 2 0.64 0 0.00 43 3.69 Yellow Perch 2 0.46 21 6.71 17 4.10 40 3.43 Channel Catfish 8 1.83 9 2.88 17 4.10 34 2.92 White Crappie 1 0.23 12 3.83 20 4.82 33 2.83 Common Carp 9 2.05 13 4.15 10 2.41 32 2.74 Striped Bass 15 3.42 15 4.79 2 0.48 32 2.74 White Catfish 9 2.05 3 0.96 11 2.65 23 1.97 Golden Shiner 0 0.00 18 5.75 2 0.48 20 1.72 Shorthead Redhorse 6 1.37 10 3.19 0 0.00 16 1.37 Hybrid Bass (Striped x White) 2 0.46 13 4.15 0 0.00 15 1.29 Satinfin Shiner 0 0.00 7 2.24 1 0.24 8 0.69 Redbreast Sunfish 2 0.46 1 0.32 3 0.72 6 0.51 Silver Redhorse 2 0.46 3 0.96 0 0.00 5 0.43 Green Sunfish 1 0.23 1 0.32 2 0.48 4 0.34 Longnose Gar 0 0.00 0 0.00 4 0.96 4 0.34 Quillback 0 0.00 0 0.00 4 0.96 4 0.34 White Bass 0 0.00 0 0.00 4 0.96 4 0.34 Flathead Catfish 0 0.00 1 0.32 2 0.48 3 0.26 Warmouth 2 0.46 0 0.00 0 0.00 2 0.17 TOTALS 811 185.08 628 200.64 397 95.7 1836 157.44 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 19 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-9. Gillnet CPUE (# of fish per 24 hour set) for fish captured in Tuckertown Tailwater. Sumner 2003 Fall 2003 Spring 2004 77AH Seasons Species # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE White Perch 192 5.49 143 5.11 58 2.07 393 4.32 Channel Catfish 91 2.60 132 4.71 56 2.00 279 3.07 Gizzard Shad 143 4.09 69 2.46 0 0.00 212 2.33 Black Crappie 21 0.60 57 2.04 20 0.71 98 1.08 Threadfin Shad 11 0.31 20 0.71 50 1.79 81 0.89 Flathead Catfish 26 0.74 3 0.11 32 1.14 61 0.67 White Crappie 42 1.20 17 0.61 0 0.00 59 0.65 Shorthead Redhorse 9 0.26 28 1.00 1 0.04 38 0.42 White Catfish 25 0.71 3 0.11 7 0.25 35 0.38 Striped Bass 0 0.00 28 1.00 5 0.18 33 0.36 Common Carp 7 0.20 3 0.11 11 0.39 21 0.23 Bluegill 11 0.31 7 0.25 2 0.07 20 0.22 Yellow Perch 0 0.00 0 0.00 20 0.71 20 0.22 Largemouth Bass 11 0.31 1 0.04 6 0.21 18 0.20 Blue Catfish 8 0.23 1 0.04 8 0.29 17 0.19 Blueback Herring 0 0.00 8 0.29 4 0.14 12 0.13 Quillback 1 0.03 7 0.25 3 0.11 11 0.12 Redear Sunfish 8 0.23 1 0.04 0 0.00 9 0.10 Hybrid Bass (Striped x White) 0 0.00 7 0.25 0 0.00 7 0.08 Silver Redhorse 0 0.00 6 0.21 0 0.00 6 0.07 Longnose Gar 3 0.09 1 0.04 1 0.04 5 0.05 Creek Chubsucker 0 0.00 1 0.04 0 0.00 1 0.01 Warmouth 1 0.03 0 0.00 0 0.00 1 0.01 White Bass 0 0.00 0 0.00 1 0.04 1 0.01 TOTALS 610 17.43 543 19.42 285 10.18 1438 15.81 (18.8%), and white perch (15.7%) were the three most abundant species, comprising over 60% of the fish captured (Table 5-15). Channel catfish, largemouth bass, and threadfin shad combined made up another 15% of the fish captured. Summer electrofishing CPUE in the Tuckertown tailwater averaged 185 fish per hour (Table 5-8). A total of 811 fish representing 21 species were captured by boat electrofishing during this effort. Gizzard shad (64.5 per hour) and bluegill (58.4 per hour) were the two most abundant fish collected in the tailwater, followed by largemouth bass (15.1 per hour), threadfm shad (9.6 per hour), and blueback herring (9.4 per hour). Gill net CPUE in Tuckertown tailwater during the summer sample period was 17.4 fish per net (Table 5-9). Six hundred and ten fish representing 17 species were captured by gill net and of these, white perch (5.5 fish per net), gizzard shad (4.1 per net), channel catfish (2.6 per net) and white crappie (1.2 per net) were the dominant species captured in the gill nets. During the summer sampling, one largemouth bass was captured by beach seining and one channel catfish was collected with the backpack electrofishing unit. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 20 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft The average PSD and RSD-P for largemouth bass was 86 and 63, respectively, and both these values exceeded the species ideal range (Table 5-10). Black crappie had an average PSD of 57 and RSD-P of 16, suggesting a balanced population with some large quality fish available. White crappie had a summer PSD of 23, slightly lower than the ideal range and an RSD-P of 9 within the ideal range, indicating some large fish are available for harvest. The average bluegill PSD (18) was slightly below the range for a balanced population, as was the average RSD-P (0). All redear sunfish captured during the summer that were greater than the minimum "stock" length for calculation of PSD values were also greater than the lower end of the "quality" size class. This resulted in a summer season PSD value of 100, which exceeded the ideal range of 20 to 60 reported for this species. The RSD-P value for redear was 32, higher than the ideal ranges, and this was due to the number of larger individuals captured. Black crappie had an average relative weight of 91 during the summer sampling season, which is slightly lower than the recommended values of 95 to 105 reported for that species (Table 5-10). The average white crappie relative weight for the summer sampling period was 86, also below the acceptable range (95-105) for the species. The average bluegill relative weight for the summer Table 5-10. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr)Values for selected species within Tuckertown Tailwater during all three sampling seasons. PSD Species Summer Fall S r?n All Seasons Ideal Range Black crappie 57 71 65 66 30 to 60* Bluegill 18 5 15 15 20 to 60* Largemouth bass 86 85 83 84 40 to 70* Redear sunfish 100 N/A 100 100 20 to 60* White crappie 23 54 34 35 30 to 60* RSD-P Summer Fall S rin All Seasons Ideal Range Black crappie 16 19 35 27 5 to 20* Bluegill 0 0 0 0 5 to 20* Largemouth bass 63 54 56 57 10 to 40* Redear sunfish 32 N/A 30 30 5 to 20* White crappie 9 25 16 16 5 to 20* Wr Summer Fall S rin All Seasons Ideal Range Black crappie 91 97 92 93 95-105** Bluegill 101 92 98 98 90-100*** Channel catfish 92 87 93 92 90-100* Largemouth bass 106 102 104 101 95-100*** Redear sunfish 78 N/A 77 78 95-100*** White crappie 86 92 87 87 95-105** *(Anderson and Neumann 1996) **(Neumann and Murray 1991) ***(Murphy et al. 1991) 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 21 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft sampling period was 101, slightly higher than the acceptable range (95-100) for the species, indicating they are in very good condition. The average relative weight for redear sunfish during the summer sampling was 78, lower then the ideal range for the species, indicating a potential problem securing food. Average largemouth bass relative weights (106) for the summer period were higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 92) were within the recommended range for the species. 5.2.2 Tuckertown Tailwater Fall Sample Period A total of 1,176 fish, representing 26 species were captured in the Tuckertown tailwater (all gear types combined) between November 9 and November 11, 2003 (Table 5-7). Bluegill (20.8%), white perch (14.0%), and channel catfish (12.0%) were the three most abundant fish captured (Table 5-8). Along with gizzard shad (9.9%), black crappie (7.1%) and largemouth bass (6.7%), these six species combined to represent over 70% of the total number of fish captured. Total electrofishing CPUE (all fish combined) in the Tuckertown tailwater was 201 fish per hour and from this effort, 628 fish, representing 23 species were captured during the fall period (Table 5-8). Bluegills were the most abundant fish captured with a CPUE of 74.4 fish per hour, followed by largemouth bass (24.6 per hour), threadfin shad (17.9 per hour), and gizzard shad (15.0 per hour). Gill net CPUE for fish collected during the fall period in Tuckertown tailwater was 19.4 fish per net (Table 5-9). A total of 543 fish, representing 21 species were captured. White perch (5.1 per net) and channel catfish (4.7 per net) comprised over 50 % of the fish caught in the gill nets. Gizzard shad (2.5 per net) and black crappie (2.0 per net) were the third and fourth most commonly captured fish. During the fall sampling, four bluegills and one largemouth bass were captured by backpack electrofishing. There were no fish captured by beach seine in Tuckertown tailwater during the fall sampling period. The average fall PSD and RSD-P for largemouth bass was 85 and 54, respectively, exceeding the ideal range for both values (Table 5-10). Black crappies also exceeded the ideal values for PSD (71) and RSD-P (19) indicating some large fish were available for harvest. White crappie had a fall PSD of 54 and an RSD-P of 25, also exceeding ideal values reported in the literature. The average bluegill PSD (5) was below the range for a balanced population, as was the average RSD-P (0). Black crappie had an average relative weight of 97 during the fall sampling season, which is within the recommended values of 95 to 105 reported for that species and the average white crappie relative weight for the fall sampling period was 92, just below the acceptable range (95-105). The average bluegill relative weight for the fall sampling period was 92, near the acceptable range (95-100) for the species. Average largemouth bass relative weights (102) for the fall period were slightly higher than the recommended range of 95 to 100 reported in the literature and channel catfish (Wr = 87) were just below the recommended range for the species. 5.2.3 Tuckertown Tailwater Spring Sample Period A total of 697 individuals, representing 25 species were captured in the Tuckertown tailwater (all gear types combined) between May 9 and May 12, 2004 (Tables 5-7). Bluegill (12.6%), largemouth bass (11.3%), channel catfish (10.5%), threadfm shad (9.9%) and white perch (9.0%) comprised the top 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 22 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft five species captured in the tailwater during the spring and accounted for 53.3 % of the individuals caught (Table 5-7). Total electrofishing CPUE averaged 95.7 fish per hour of effort during the Tuckertown spring sampling event and a total of 397 individuals representing 22 species were captured (Table 7-8). Bluegill (17.6 per hour), largemouth bass (17.6 per hour), pumpkinseed (15.0 per hour) and gizzard shad (12.8 per hour) were the four most abundant species collected, comprising 66% of the fish captured electrofishing. Total CPUE (all fish combined) for gill nets fished during May of 2004 was 10.2 fish per net (Table 5-9). A total of 285 individuals representing 17 species were caught in gill nets and of these, white perch (2.1 per net), channel catfish (2.0 per net), threadfin shad (1.8 per net), and flathead catfish (1.1 per net) were the dominant fish. During the spring sampling, 1 golden shiner, 1 flathead catfish and 1 bluegill were captured by backpack electrofishing and 12 bluegills were captured by beach seine in Tuckertown tailwater. The average PSD and RSD-P for largemouth bass was 83 and 56, respectively, exceeding the ideal range for both values (Table 5-10). Black crappie had an average PSD of 65 and RSD-P of 35 and white crappie had a PSD of 34 and an RSD-P of 16. Both crappie species exceeded the ideal ranges for these values, indicating balanced fish populations with some quality sized fish available. The average bluegill PSD (15) was below the range for a balanced population, as was the average RSD-P (0). All redear sunfish captured during the spring that were greater than the minimum "stock" length for calculation of PSD values were also greater than the lower end of the "quality" size class. This resulted in a spring season PSD value of 100, well over the ideal range, and an RSD-P value of 30, also greater than the ideal range of 5 to 20 recommended for this fish, indicating some large quality fish are available for harvest. Black crappie had an average relative weight of 92 during the spring sampling season, which is slightly lower than the recommended values of 95 to 105 reported for that species. The average white crappie relative weight for the spring sampling period was 87, also below the acceptable range (95- 105) for the species. The average bluegill relative weight for the spring sampling period was 98, within the acceptable range (95-100) for the species. The average relative weight for redear sunfish during the spring sampling was 77, lower then the ideal range for the species. Average largemouth bass relative weights (104) for the spring period were slightly higher than the recommended range of 95 to 100 reported in the literature and channel catfish (Wr = 93) were within the recommended range for the species. 5.2.4 Seasonal Differences in Tuckertown Tailwater Species richness was highest in the Tuckertown tailwater during the November sampling period. Bluegill, largemouth bass, channel catfish, threadfin shad, white perch and gizzard shad were consistently within the top seven species collected (all gear types combined) for each of the three sampling periods. The PSD, RSD-P and Wr values of largemouth bass were similar to values suggested by Anderson (1980) to be representative of a population that either has low or no annual reproduction or low rates of mortality on quality-sized fish. Since multiple size classes of largemouth bass were present (see Appendix 2 Figure 1-10), the data suggests that large, quality sized fish have low mortality and this may be a result of catch and release fishing. The PSD and RSD-P values for Tuckertown tailwater bluegills suggest a population that is not balanced due to few large fish, 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 23 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft however, the bluegills captured had relative weights within the ideal range, suggesting the fish were in good condition. Redear sunfish were not present in the tailwater in great enough numbers during the fall season to calculate PSD/RSD-P values. The Wr values for this species (<78) were lower than optimum in both the spring and summer periods and this may be suggestive of a problem with food or feeding in this species. Relative weights for all six species looked at were consistent throughout the three seasons. Appendix 2 (Figures 1-10 through 1-21) show the length frequency distributions for several important game and forage fish found in the Tuckertown tailwater. Largemouth bass representing all the size classes from 2-50 cm were captured during all three sampling periods in Tuckertown, demonstrating that successful spawning is occurring. Adult and juvenile sized striped bass were present in Tuckertown tailwater during all three sampling seasons. Fingerling striped bass are currently stocked by NCWRC into Narrows Reservoir (Tuckertown tailwater) at a rate of 62,000 fish per year. Blueback herring were present in Tuckertown tailwater during all three seasons sampled as a result of stockings into Narrows Reservoir (Tuckertown tailwater) during the 1970's. The presence of juvenile sized fish in the fall sampling suggests that this species is spawning successfully and continues to maintain a small land-locked population. Black and white crappies of different size classes were present in the tailwater during all three sampling events, indicating these fish are successfully spawning in Narrows reservoir (Tuckertown tailwater). Bluegills were present in large numbers and over a range of size classes demonstrating that they are successfully spawning. While PSD and RSD- P values are low for bluegill, suggesting an abundance of small individuals, the average relative weight value of all fish captured during the three seasons of sampling is within the ideal range for the species suggesting feeding is not a problem. The presence of small redear sunfish during the fall sampling period suggests that these fish are spawning and maintaining a population within Tuckertown tailwater. Channel and blue catfish are maintaining populations in the tailwater with some individuals reaching large size. Large numbers of forage species such as white perch, gizzard shad and threadfin shad demonstrate that these fish are spawning successfully. 5.3 NARROWS TAILWATER FISHERIES This section presents results of all the fish captured in the Narrows tailwater during the three sampling periods. Figure 5-3 shows the seasonal gill net locations, the extent of the area that was electrofished by boat, and the locations of backpack electrofishing and beach seining stations. A total of 3,667 fish (all gear types and seasons combined) representing 30 species were captured in the Narrows tailwater over the three seasons of sampling (Tables 5-11 & 5-12). White perch (20.3 gizzard shad (19.6%) and bluegill (12.6%) were the top three fish species captured in Narrows during the study. Most of these fish (2,417 individuals) were captured by electrofishing during all three seasons of sampling. Gizzard shad (CPUE=39.2 fish/hr) were the most commonly captured species (Table 5-13). A total of 1,217 fish were captured by gill nets during the three seasons of sampling and of these, white perch were the dominant species with a CPUE of 6.9 per net (Table 5-14). A total of 25 individuals representing six species (bluegill, black crappie, golden shiner, largemouth bass, pumpkinseed, and redear sunfish) were captured by beach seine during all three sampling seasons. Backpack shocking yielded eight individuals representing five species (bluegill, gizzard shad, largemouth bass, pumpkinseed, and redbreast sunfish). 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 24 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 5-3. Narrows Tailwater Fisheries Sampling Locations. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 25 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-11. Species composition for Narrows Tailwater by season. Sampling Periods Common Name Scientific Name Summer 2003 Fall 2003 S rin 2004 All Seasons Blueback Herring Alosa aestivalis x X X X Gizzard Shad Dorosoma cepedianum x X X X Threadfin Shad Dorosoma petenense x X X X Common Carp Cyprinus carpio x X X X Golden Shiner Notemigonus chrysoleucas x X X Satinfin Shiner Cyprinella analostana x X X Shorthead Redhorse Moxostoma macrolepidotum x X X X Silver Redhorse Moxostoma anisurum x X White Catfish Ameiurus catus x X X X Channel Catfish ktalurus puntatus x X X X Flathead Catfish Pylodictus olivarus x X X X White Perch Morone americana x X X X Striped Bass Morone saxatilis x X X X White Bass Morone chrysops x X Redbreast Sunfish Lepomis auritus x X X X Green Sunfish Lepomis cyanellus x X X X Pumpkinseed Lepomis gibbosus x X X X Bluegill Lepomis macrochirus x X X X Largemouth Bass Micropterus salmoides x X X X White Crappie Pomoxis annularis x X X Black Crappie Pomoxis nigromaculatus x X X Yellow Perch Perca flavescens x X X X Bowfin Amia Galva x X Longnose Gar Lepisosteus osseus x X X Flat Bullhead Ameiurus platycephalus x X X X Warmouth Lepomis gulosus x X X X Smallmouth Buffalo ktiobus bubalus x X Snail Bullhead Ameiurus brunneus x X Blue Catfish ktalurus furcatus x X X X Redear Sunfish Lepomis microlophus x X X X Total Taxa 23 24 28 30 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 26 Normandeau Associates, inc. Table 5-12. Percent Composition of Fish Species Captured within Narrows Tailwater, all gear types combined. N J Summer 2003 Fall 2003 S "n 2004 All Seasons _Species # Individuals % Com position # Individuals % Com position # Individuals % Composition # Individuals % Composition White Perch 505 23.36% 217 24.27% 23 3.76% 745 20.32% Gizzard Shad 553 25.58% 90 10.07% 77 12.60% 720 19.63% Bluegill 273 12.63% 90 10.07% 100 16.37% 463 12.63% Largemouth Bass 245 11.33% 125 13.98% 52 8.51% 422 11.51% Redbreast Sunfish 198 9.16% 111 12.42% 89 14.57% 398 10.85% Channel Catfish 92 4.26% 38 4.25% 73 11.95% 203 5.54% White Catfish 67 3.10% 24 2.68% 42 6.87% 133 3.63% Blue Catfish 90 4.16% 7 0.78% 24 3.93% 121 3.30% Threadfin Shad 17 0.79% 58 6.49% 6 0.98% 81 2.21% Blueback Herring 2 0.09% 41 4.59% 18 2.95% 61 1.66% Warmouth 23 1.06% 10 1.12% 23 3.76% 56 1.53% Striped Bass 14 0.65% 18 2.01% 7 1.15% 39 1.06% Flathead Catfish 19 0.88% 4 0.45% 12 1.96% 35 0.95% Redear Sunfish 16 0.74% 5 0.56% 14 2.29% 35 0.95% Black Crappie 15 0.69% 16 1.79% 1 0.16% 32 0.87% Pumpkinseed 6 0.28% 11 1.23% 15 2.45% 32 0.87% Yellow Perch 11 0.51% 5 0.56% 11 1.80% 27 0.74% Common Carp 5 0.23% 5 0.56% 6 0.98% 16 0.44% Shorthead Redhorse 1 0.05% 7 0.78% 2 0.33% 10 0.27% Longnose Gar 0 0.00% 6 0.67% 2 0.33% 8 0.22% Golden Shiner 6 0.28% 0 0.00% 1 0.16% 7 0.19% Flat Bullhead 2 0.09% 1 0.11% 3 0.49% 6 0.16% Green Sunfish 1 0.05% 2 0.22% 1 0.16% 4 0.11% Snail Bullhead 0 0.00% 0 0.00% 3 0.49% 3 0.08% Satinfin Shiner 1 0.05% 0 0.00% 1 0.16% 2 0.05% Silver Redhorse 0 0.00% 0 0.00% 2 0.33% 2 0.05% Smallmouth Buffalo 0 0.00% 0 0.00% 2 0.33% 2 0.05% White Bass 0 0.00% 1 0.11% 1 0.16% 2 0.05% Bowfin 0 0.00% 1 0.11% 0 0.00% 1 0.03% White Crappie 0 0.00% 1 0.11% 0 0.00% 1 0.03% TOTALS 2162 100.00% 894 100.00% 611 100.00% 3667 100.00% rz) Tailwater Fish & Aquatic Biota Assessment Draft Table 5-13. Electrofishing CPUE (# of fish per hour of shock time) for fish captured in Narrows Tailwater. Summer 2003 Fall 2003 Spring 2004 777AR Seasons S ecies # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE Gizzard Shad 386 64.76 62 15.72 77 22.12 525 39.22 Bluegill 258 43.29 83 21.04 100 28.72 441 32.95 Redbreast Sunfish 197 33.05 108 27.38 89 25.56 394 29.43 Largemouth Bass 228 38.25 112 28.40 51 14.65 391 29.21 White Perch 85 14.26 12 3.04 23 6.61 120 8.96 White Catfish 51 8.56 23 5.83 36 10.34 110 8.22 Channel Catfish 39 6.54 14 3.55 46 13.21 99 7.40 Threadfin Shad 17 2.85 41 10.40 6 1.72 64 4.78 Blueback Herring 2 0.34 41 10.40 1 0.29 44 3.29 Warmouth 18 3.02 7 1.77 16 4.60 41 3.06 Redear Sunfish 14 2.35 5 1.27 14 4.02 33 2.47 Yellow Perch 11 1.85 5 1.27 10 2.87 26 1.94 Pumpkinseed 5 0.84 5 1.27 15 4.31 25 1.87 Striped Bass 10 1.68 12 3.04 2 0.57 24 1.79 Black Crappie 9 1.51 6 1.52 1 0.29 16 1.20 Blue Catfish 13 2.18 1 0.25 0 0.00 14 1.05 Common Carp 3 0.50 4 1.01 5 1.44 12 0.90 Flathead Catfish 2 0.34 2 0.51 6 1.72 10 0.75 Golden Shiner 4 0.67 0 0.00 1 0.29 5 0.37 Green Sunfish 1 0.17 2 0.51 1 0.29 4 0.30 Flat Bullhead 2 0.34 0 0.00 1 0.29 3 0.22 Shorthead Redhorse 0 0.00 2 0.51 1 0.29 3 0.22 Snail Bullhead 0 0.00 0 0.00 3 0.86 3 0.22 Longnose Gar 0 0.00 0 0.00 2 0.57 2 0.15 Satinfin Shiner 1 0.17 0 0.00 1 0.29 2 0.15 Smallmouth Buffalo 0 0.00 0 0.00 2 0.57 2 0.15 Bowfin 0 0.00 1 0.25 0 0.00 1 0.07 Silver Redhorse 0 0.00 0 0.00 1 0.29 1 0.07 White Bass 0 0.00 0 0.00 1 0.29 1 0.07 White Crappie 0 0.00 1 0.25 0 0.00 1 0.07 TOTALS 1356 227.52 549 139.19 512 147.07 2417 180.55 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 28 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-14. Gillnet CPUE (# of fish per 24 hour set) for fish captured in Narrows Tailwater. Sumner 2003 - Fall 2003 7 -7 S rin 2004 All Seasons S ecies # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE White Perch 420 8.75 205 8.54 0 0.00 625 6.94 Gizzard Shad 167 3.48 27 1.13 0 0.00 194 2.16 Blue Catfish 77 1.60 6 0.25 24 1.33 107 1.19 Channel Catfish 53 1.10 24 1.00 27 1.50 104 1.16 Flathead Catfish 17 0.35 2 0.08 6 0.33 25 0.28 Largemouth Bass 10 0.21 13 0.54 0 0.00 23 0.26 White Catfish 16 0.33 1 0.04 6 0.33 23 0.26 Blueback Herring 0 0.00 0 0.00 17 0.94 17 0.19 Threadfin Shad 0 0.00 17 0.71 0 0.00 17 0.19 Black Crappie 5 0.10 10 0.42 0 0.00 15 0.17 Striped Bass 4 0.08 6 0.25 5 0.28 15 0.17 Warmouth 5 0.10 3 0.13 7 0.39 15 0.17 Bluegill 3 0.06 7 0.29 0 0.00 10 0.11 Shorthead Redhorse 1 0.02 5 0.21 1 0.06 7 0.08 Longnose Gar 0 0.00 6 0.25 0 0.00 6 0.07 Common Carp 2 0.04 1 0.04 1 0.06 4 0.04 Flat Bullhead 0 0.00 1 0.04 2 0.11 3 0.03 Redbreast Sunfish 0 0.00 3 0.13 0 0.00 3 0.03 Redear Sunfish 1 0.02 0 0.00 0 0.00 1 0.01 Silver Redhorse 0 0.00 0 0.00 1 0.06 1 0.01 White Bass 0 0.00 1 0.04 0 0.00 1 0.01 Yellow Perch 0 0.00 0 0.00 1 0.06 1 0.01 TOTALS 781 16.24 338 14.09 98 5.45 1217 13.54 5.3.1 Narrows Tailwater Summer Sample Period A total of 2,162 fish, representing 23 species were captured in the Narrows tailwater (all gear types combined) between August 28 and September 1, 2003 (Table 5-12). Gizzard shad and white perch were the dominant fish, and together they represented 50% of the individuals captured (Table 5-12). Bluegill (12.6%), largemouth bass (11.3%), redbreast sunfish (9.2%) and channel catfish (4.3%) rounded out the top six species captured. Total summertime electrofishing CPUE in Narrows tailwater was 227.5 fish per hour, with a total of 1,356 fish collected (Table 5-13). Gizzard shad were the most frequently captured fish in the summer electrofish samples, with a CPUE of 64.8 fish caught per hour, followed by bluegill (43.3 per hour), largemouth bass (38.3 per hour) and redbreast sunfish (33.1 per hour). The above four species along with white perch (14.3 per hour) and white catfish (8.6 per hour) comprised the top six fish captured by electrofishing. Total CPUE for fish (all species combined) collected in the Narrows gill nets during the summer of 2003 was 16.2 fish per 24 hour set (Table 5-14). White perch were the most abundant fish in the gill nets with a CPUE of 8.8 fish per net followed by gizzard shad with 3.5 fish per net. Four catfish 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 29 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft species, blue (CPUE=1.6), channel (L 1), flathead (0.4) and white (0.3), rounded out the six most frequently captured species in the gillnets. During the summer sampling period in Narrows tailwater, a total of 19 fish representing five species were captured by beach seine (9 bluegill, 6 largemouth bass, 2 golden shiner, 1 black crappie, 1 redear sunfish). Backpack electrofishing captured a total of 6 fish representing four species (3 bluegill, 1 redbreast sunfish, 1 pumpkinseed, l largemouth bass). The average PSD and RSD-P for largemouth bass was 84 and 41 respectively and these exceed the species ideal range (Table 5-15). Black crappie had an average PSD of 93 and RSD-P of 57, both values being higher then the optimum ranges for the species. The average bluegill PSD (17) was just below the range for a balanced population, as was the average RSD-P (0). Redear sunfish captured during the summer had an average PSD value of 33 and RSD-P value of 33. The PSD value was within the optimum range for the species whereas the RSD-P value was above, indicating some large fish were captured. Table 5-15. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr) Values for selected species within Narrows Tailwater during all three sampling seasons. PSD Species Summer Fall S r?n All Seasons Ideal Range Black crappie 93 100 N/A 97 30 to 60* Bluegill 17 24 21 20 20 to 60* Largemouth bass 84 70 79 78 40 to 70* Redear sunfish 33 N/A 61 53 20 to 60* RSD-P Summer Fan S r?n All Seasons Ideal Range Black crappie 57 93 N/A 76 5 to 20* Bluegill 0 0 0 0 5 to 20* Largemouth bass 41 38 39 39 10 to 40* Redear sunfish 33 N/A 35 35 5 to 20* Wr Summer Fan S r?n All Seasons Ideal Range Black crappie 91 99 96 95 95 to 105** Blue catfish 108 N/A 106 107 90 to 100* Bluegill 93 89 92 92 95 to 100*** Channel catfish 99 98 100 99 90 to 100* Largemouth bass 104 108 104 102 95 to 100*** Redear sunfish 83 N/A 79 80 95 to 100*** *(Anderson and Neumann 1996) **(Neumann and Murray 1991) ***(Murphy et al. 1991) Table 5-15 shows the average relative weights for six fish species captured in Narrows tailwater by season. Black crappie had an average relative weight of 91 during the summer sampling season, which is lower than the recommended values of 95 to 105 reported for that species. The average bluegill relative weight for the summer sampling period was 93, near the acceptable range (95-100) 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 30 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft for the species. The average relative weight for redear sunfish during the summer sampling was 83, lower than the ideal range for the species. Average largemouth bass relative weights (104) for the summer period were slightly higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 99) were within the recommended range for the species whereas blue catfish (Wr = 108) were higher then the range suggested for that species. 5.3.2 Narrows Tailwater Fall Sample Period A total of 894 fish, representing 24 species were captured in the Narrows tailwater (all gear types combined) between November 6 and November 8, 2003 (Table 5-12). White perch were the most frequently caught, comprising 24 % of the catch , followed by largemouth bass (14.0 %), redbreast sunfish (12.4 %), bluegill (10.1 %) and gizzard shad (10.1 %). These five species made up over 70 % of the fish caught during the fall period. Electrofishing CPUE in Narrows tailwater averaged 139.2 fish per hour, with a total of 549 fish collected (Table 5-13). Largemouth bass (28.4 per hour), redbreast sunfish (27.4 per hour) and bluegill (21.0 per hour) were the most abundant fish captured by electrofishing. Total CPUE for fish collected in the Narrows gill nets during November of 2003 was 14.1 fish per 24 hour set (Table 5-14). A total of 338 individuals were captured and of these, white perch were the dominant fish captured in the gillnets, with CPUE of 8.5 fish per net. Gizzard shad (1.1 per net), channel catfish (1.0 per net), threadfm shad (0.7 per net) and largemouth bass (0.5 per net) rounded out the five most commonly captured fish in gillnets. During the fall sampling in Narrows tailwater, a total of six pumpkinseed were captured by beach seine. A single gizzard shad was captured by backpack electrofishing. The average PSD and RSD-P for largemouth bass was 70 and 38 respectively and black crappie had an average PSD of 100 and RSD-P of 93 (Table 5-15). These values are higher than the optimum ranges for both species. The average bluegill PSD (24) was within the range for a balanced population, whereas the average RSD-P (0) was below. Redear sunfish were not captured in high enough numbers during the fall sampling period to calculate stock densities or relative weights. Black crappie had an average relative weight of 99 during the fall sampling season, which is within the recommended range of 95 to 105 reported for that species (Table 5-15). The average bluegill relative weight for the fall sampling period was 89, slightly below the acceptable range (95-100) for the species. Average largemouth bass relative weights (108) for the fall period were slightly higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 98) were within the recommended range for the species. 5.3.3 Narrows Tailwater Spring Sample Period A total of 611 fish, representing 28 species were captured in the Narrows tailwater (all gear types combined) between May 12 and May 14, 2004 (Table 5-12). The five dominant fish captured in the Narrows tailwater during the spring sample period were bluegill (16.4 %), redbreast sunfish (14.6 gizzard shad (12.6 %), channel catfish (12.0 %) and largemouth bass (8.5 %). Electrofishing CPUE in Narrows tailwater averaged 147 fish per hour, with a total of 512 fish collected (Table 5-13). Similar to the fall season, bluegill (28.7 per hour) and redbreast sunfish (25.6 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 31 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft per hour) were the two dominant species, followed by gizzard shad (22.1 per hour) largemouth bass (14.7 per hour), and channel catfish (13.2 per hour). Total CPUE for fish collected from gillnets during the spring season was 5.44 fish per net and from this effort, 98 fish, representing twelve species were captured (Table 5-14). Channel catfish (1.5 per net), blue catfish (1.3 per net), and blueback herring were the three most frequently captured species in gillnets. During the spring sampling in Narrows tailwater, one largemouth bass was captured by backpack electrofishing. There were no fish captured by beach seine in Narrows tailwater during the spring sampling. The average PSD and RSD-P for largemouth bass was 79 and 39 respectively, exceeding the species optimum range (Table 5-15). The average bluegill PSD (21) was within the range for a balanced population, but the average RSD-P was zero due to no large fish. Channel catfish had a spring season PSD of 58 and an RSD-P of 2. Blue catfish had a spring season PSD of 26 and an RSD-P of 1. Redear sunfish captured during the spring had an average PSD value of 61 and RSD-P value of 35, with both values exceeding the optimum range. Black crappie sample size for the spring season was too small for calculation of average stock densities. The average bluegill relative weight for the spring sampling period was 100, within the acceptable range (95-100) for the species, while the average relative weight for redear sunfish during the spring sampling was 79, lower then the ideal range for the species (Table 5-15). Average largemouth bass relative weight (104) for the spring period was slightly higher than the recommended range of 95 to 100 reported in the literature. Channel catfish (Wr = 100) were within the recommended range for the species whereas blue catfish (Wr = 106) were higher then the range suggested for that species. 5.3.4 Seasonal Differences in Narrows Tailwater Species abundance was highest in Narrows tailwater during the spring season. Largemouth bass, bluegill, redbreast sunfish, white perch, and gizzard shad were consistently among the five most abundant species sampled during all three seasons. Blueback herring were represented in the catch during all three seasons. These fish have not been officially stocked into Falls reservoir (Narrows tailwater) but have most likely been recruited from the land-locked population found upstream in Narrows reservoir. The PSD and RSD values for largemouth bass and bluegill in Narrows tailwater are consistent through all three sampling periods. Overall, largemouth bass and bluegill were within or near the suggested ranges, indicating a balanced population. Redear sunfish were not present in the tailwater in great enough numbers during the fall season to calculate PSD/RSD-P values. The values for this species were lower than suggested in both the spring and summer and this could be suggestive of a problem with food or feeding in this species. Relative weights for all six species calculated were consistent throughout the three seasons. Appendix 2 (Figures 1-22 through 1-32) show length frequency distributions for several important game and forage fish found in Narrows tailwater during all three sampling seasons. Largemouth bass are successfully spawning as they show a bimodal length frequency distribution with both young fish and older spawning age adults represented in the catches. Adult and juvenile sized striped bass were present in the Narrows tailwater during all three seasons. The NCWRC does not currently stock striped bass into Falls Reservoir (Narrows tailwater) and the presence of these fish in Falls reservoir is most likely due to successful recruitment from upstream sources. Small numbers of blueback 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 32 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft herring were captured in the Narrows tailwater during all three seasons and their presence could be explained by recruitment from the established population upsystem in Narrows Reservoir. Redear sunfish appear to be successfully spawning in the tailwater as shown by the variety of size classes present. They are not as abundant in Narrows tailwater as they were in the Falls tailwater. Bluegills in Narrows tailwater seem to be dominated by small fish in the 12 - 14 cm range. Black crappie were captured in small numbers in the Narrows tailwater and the presence of juvenile size classes could suggest some spawning taking place in Falls Reservoir (Narrows tailwater) or possible recruitment from the upstream population in Narrows Reservoir. The presence of individuals in many size classes indicates that both channel and blue catfish are spawning and achieving large sizes within the Falls reservoir. Three major forage species, gizzard shad, threadfin shad and white perch were abundant in the tailwater demonstrating successful spawning. 5.4 FALLS TAILWATER FISHERIES This section presents results of all the fish captured in the Falls tailwater during the three sampling periods. Figure 5-4 shows the seasonal gill net locations, the extent of the area that was electrofished by boat, and the locations of backpack electrofishing and beach seining stations. A total of 2,906 individuals (all gear types and seasons combined) representing 34 species were captured in the Falls tailwater over the three seasons of sampling (Table 5-16 & 5-17). Over all three seasons of sampling, gizzard shad (13.7 %), redbreast sunfish (13.5 %), and bluegill (12.5 %) were the top three fish species captured (Table 5-17). A total of 2,395 fish were captured by electrofishing during all three seasons of sampling. Redbreast sunfish were the most commonly captured species with 23.9 fish captured per hour (Table 5-18). A total of 357 fish were captured by gillnets during all three seasons of sampling and of these white perch were dominant with 1.1 captured per 24 hour set (Table 5-19). A total of seven individuals representing four species were captured by backpack electrofishing during all three sampling seasons (bluegill, largemouth bass, redbreast sunfish, and tessellated darter). Beach seining yielded three individuals representing two species (bluegill and redbreast sunfish). 5.4.1 Falls Tailwater Summer Sample Period A total of 834 fish representing 23 species were captured in Falls tailwater (all gear types combined) between August 26 and August 27, 2003 (Table 5-17). Bluegill (16 %) were the dominant fish species, followed by white perch (13 %), threadfin shad (10 %), gizzard shad (10 %), and redbreast sunfish (10 ON). Two game species, the redear sunfish and largemouth bass, each comprised between 7 and 8 % of the capture. These top seven fish species represented more then 75 % of the catch in Falls tailwater. Electrofishing CPUE in the Falls tailwater averaged 168.4 fish per hour, with a total of 676 fish collected (Table 5-18). Bluegills were the dominant species with 32.1 fish per hour followed by white perch (21.9 per hour), redbreast sunfish (19.9 per hour), redear sunfish (15.4 per hour), gizzard shad, and threadfin shad (both at 15.2 per hour). CPUE for fish collected by the gillnets in the Falls tailwater during August of 2003 was 3.3 fish per net, with a total of 157 fish captured (Table 5-19). Blue catfish CPUE was 0.6 per net, followed by threadfin shad (0.5 per net), gizzard shad (0.5 per net) and white perch (0.4 per net). 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 33 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 5-4. Falls Tailwater Fisheries Sampling Locations. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 34 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-16. Species composition for Falls Tailwater by season. Sampling Periods Common Name Scientific Name Summer 2003 Fall 2003 S rin 2004 All Seasons Blueback Herring Alosa aestivalis x X X X Gizzard Shad Dorosoma cepedianum x X X X Threadfin Shad Dorosoma petenense x X X X Golden Shiner Notemigonus chrysoleucas x X Satinfin Shiner Cyprinella analostana x X X X Quillback Carpiodes cyprinus x X White Sucker Catostomus commersoni x X Creek Chubsucker Erimyzon oblongus x X X Shorthead Redhorse Moxostoma macrolepidotum x X X X Silver Redhorse Moxostoma anisurum x X X X White Catfish Ameiurus catus x X X X Yellow Bullhead Ameiurus natalis x X X Channel Catfish ktalurus puntatus x X X X Flathead Catfish Pylodictus olivarus x X X White Perch Morone americana x X X X Striped Bass Morone saxatilis x X X X White Bass Morone chrysops x X Redbreast Sunfish Lepomis auritus x X X X Green Sunfish Lepomis cyanellus x X Pumpkinseed Lepomisgibbosus x X X X Bluegill Lepomis macrochirus x X X X Smallmouth Bass Micropterus dolomieu x X Largemouth Bass Micropterus salmoides x X X X White Crappie Pomoxis amrularis x X Black Crappie Pomoxis nigromaculatus x X X Tesselated Darter Etheostome olmstedi x X X Yellow Perch Perca flavescens x X X X Longnose Gar Lepisosteus osseus x X X X Flat Bullhead Ameiurus platycephalus x X Warmouth Lepomis gulosus x X X X Spotted Sucker Minytrema melanops x X X X Snail Bullhead Ameiurus brunneus x X X Blue Catfish ktalurus furcatus x X X X Redear Sunfish Lepomis microlophus x X X X Total Taxa 23 27 30 34 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 35 Normandeau Associates, inc. Table 5-17. Percent Composition of Fish Species Captured within Falls Tailwater, all gear types combined. w Summer 2003 Fall 2003 Spring 2004 All Seasons Species # Individuals % Composition # Individuals % Composition # Individuals % Composition # Individuals % Composition Gizzard Shad 86 10.31% 220 18.55% 92 10.38% 398 13.70% Redbreast Sunfish 84 10.07% 205 17.28% 102 11.51% 391 13.45% Bluegill 137 16.43% 146 12.31% 81 9.14% 364 12.53% White Perch 107 12.83% 130 10.96% 76 8.58% 313 10.77% Largemouth Bass 59 7.07% 95 8.01% 102 11.51% 256 8.81% Redear Sunfish 67 8.03% 87 7.34% 55 6.21% 209 7.19% Threadfin Shad 87 10.43% 23 1.94% 74 8.35% 184 6.33% White Catfish 22 2.64% 33 2.78% 69 7.79% 124 4.27% Silver Redhorse 16 1.92% 28 2.36% 58 6.55% 102 3.51% Shorthead Redhorse 13 1.56% 68 5.73% 18 2.03% 99 3.41% Yellow Perch 28 3.36% 33 2.78% 34 3.84% 95 3.27% Blue Catfish 40 4.80% 19 1.60% 10 1.13% 69 2.37% Channel Catfish 13 1.56% 12 1.01% 19 2.14% 44 1.51% Satinfin Shiner 9 1.08% 11 0.93% 21 2.37% 41 1.41% Flathead Catfish 14 1.68% 13 1.10% 12 1.35% 39 1.34% Snail Bullhead 0 0.00% 7 0.59% 25 2.82% 32 1.10% Spotted Sucker 19 2.28% 1 0.08% 1 0.11% 21 0.72% Pumpkinseed 8 0.96% 7 0.59% 3 0.34% 18 0.62% Striped Bass 2 0.24% 15 1.26% 1 0.11% 18 0.62% Yellow Bullhead 12 1.44% 0 0.00% 1 0.11% 13 0.45% Black Crappie 2 0.24% 8 0.67% 1 0.11% 11 0.38% Blueback Herring 1 0.12% 3 0.25% 7 0.79% 11 0.38% Warmouth 4 0.48% 5 0.42% 1 0.11% 10 0.34% Tesselated Darter 0 0.00% 7 0.59% 3 0.34% 10 0.34% Longnose Gar 3 0.36% 2 0.17% 2 0.23% 7 0.24% Golden Shiner 0 0.00% 0 0.00% 6 0.68% 6 0.21% Creek Chubsucker 0 0.00% 2 0.17% 2 0.23% 4 0.14% Quillback 0 0.00% 0 0.00% 3 0.34% 3 0.10% White Bass 0 0.00% 0 0.00% 3 0.34% 3 0.10% White Sucker 0 0.00% 3 0.25% 0 0.00% 3 0.10% White Crappie 1 0.12% 1 0.08% 0 0.00% 2 0.07% Flat Bullhead 0 0.00% 0 0.00% 2 0.23% 2 0.07% Green Sunfish 0 0.00% 0 0.00% 2 0.23% 2 0.07% Smallmouth Bass 0 0.00% 2 0.17% 0 0.00% 2 0.07% TOTALS 834 100.00% 1186 100.00% 886 100.00% 2906 100.00% rz) Tailwater Fish & Aquatic Biota Assessment Draft Table 5-18. Electrofishing CPUE (# of fish per hour of shock time) for fish captured in Falls Tailwater. Summer 2003 Fall 2003 S r?n 2004 All Seasons Species # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE Redbreast Sunfish 80 1993 . 203 48.26 101 12.82 384 23.85 Bluegill 129 32.13 140 33.28 81 10.28 350 21.74 Gizzard Shad 61 15.19 178 42.32 81 10.28 320 . 1988 Largemouth Bass 48 11.96 90 21.40 100 12.69 238 14.78 Redear Sunfish 62 15.44 87 2068 . 55 6.98 204 12.67 White Perch 88 21.92 30 7.13 51 6.47 169 10.50 Threadfin Shad 61 15.19 22 5.23 54 6.85 137 8.51 Yellow Perch 28 6.97 33 7.85 34 4.32 95 5.90 Silver Redhorse 15 3.74 16 3.80 55 6.98 86 5.34 White Catfish 17 4.23 17 4.04 46 5.84 80 4.97 Shorthead Redhorse 12 2.99 50 11.89 17 2.16 79 4.91 Satinfin Shiner 9 2.24 11 2.62 21 2.67 41 2.55 Snail Bullhead 0 0.00 5 1.19 24 3.05 29 1.80 Flathead Catfish 9 2.24 12 2.85 7 0.89 28 1.74 Channel Catfish 3 0.75 5 1.19 12 1.52 20 1.24 Blue Catfish 10 2.49 0 0.00 8 1.02 18 1.12 Spotted Sucker 17 4.23 1 0.24 0 0.00 18 1.12 Pumpkinseed 7 1.74 7 1.66 3 0.38 17 1.06 Striped Bass 2 0.50 10 2.38 1 0.13 13 0.81 Yellow Bullhead 12 2.99 0 0.00 1 0.13 13 0.81 Blueback Herring 1 0.25 3 0.71 7 0.89 11 0.68 Tesselated Darter 0 0.00 5 1.19 3 0.38 8 0.50 Black Crappie 2 0.50 4 0.95 1 0.13 7 0.43 Golden Shiner 0 0.00 0 0.00 6 0.76 6 0.37 Warmouth 2 0.50 2 0.48 1 0.13 5 0.31 Creek Chubsucker 0 0.00 2 0.48 2 0.25 4 0.25 Green Sunfish 0 0.00 0 0.00 2 0.25 2 0.12 Longnose Gar 0 0.00 0 0.00 2 0.25 2 0.12 Quillback 0 0.00 0 0.00 2 0.25 2 0.12 Smallmouth Bass 0 0.00 2 0.48 0 0.00 2 0.12 White Bass 0 0.00 0 0.00 2 0.25 2 0.12 White Crappie 1 0.25 1 0.24 0 0.00 2 0.12 White Sucker 0 0.00 2 0.48 0 0.00 2 0.12 Flat Bullhead 0 0.00 0 0.00 1 0.13 1 0.06 TOTALS 676 168.37 938 223.02 781 99.13 2395 148.74 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 37 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-19. Gillnet CPUE (# of fish per 24 hour set) for fish captured in Falls Tailwater. Summer 2003 Fall 2003 S rin 20 04 All Seasons S ecies # Individuals CPUE # Individuals CPUE # Individuals CPUE # Individuals CPUE White Perch 19 0.40 100 4.17 25 0.39 144 1.06 Gizzard Shad 25 0.52 42 1.75 11 0.17 78 0.57 Blue Catfish 30 0.63 19 0.79 2 0.03 51 0.38 Threadfin Shad 26 0.54 1 0.04 20 0.31 47 0.35 White Catfish 5 0.10 16 0.67 23 0.36 44 0.32 Channel Catfish 10 0.21 7 0.29 7 0.11 24 0.18 Shorthead Redhorse 1 0.02 18 0.75 1 0.02 20 0.15 Largemouth Bass 11 0.23 5 0.21 1 0.02 17 0.13 Silver Redhorse 1 0.02 12 0.50 3 0.05 16 0.12 Flathead Catfish 5 0.10 1 0.04 5 0.08 11 0.08 Bluegill 8 0.17 2 0.08 0 0.00 10 0.07 Longnose Gar 3 0.06 2 0.08 0 0.00 5 0.04 Redear Sunfish 5 0.10 0 0.00 0 0.00 5 0.04 Striped Bass 0 0.00 5 0.21 0 0.00 5 0.04 Warmouth 2 0.04 3 0.13 0 0.00 5 0.04 Black Crappie 0 0.00 4 0.17 0 0.00 4 0.03 Redbreast Sunfish 3 0.06 0 0.00 1 0.02 4 0.03 Snail Bullhead 0 0.00 2 0.08 1 0.02 3 0.02 Spotted Sucker 2 0.04 0 0.00 1 0.02 3 0.02 Flat Bullhead 0 0.00 0 0.00 1 0.02 1 0.01 Pumpkinseed 1 0.02 0 0.00 0 0.00 1 0.01 Quillback 0 0.00 0 0.00 1 0.02 1 0.01 White Bass 0 0.00 0 0.00 1 0.02 1 0.01 White Sucker 0 0.00 1 0.04 0 0.00 1 0.01 TOTALS 157 3.26 240 10.00 104 1.66 501 3.72 There were no fish captured by backpack electrofishing in the Falls tailwater during the summer sampling period. One redbreast sunfish was collected by beach seine. The PSD and RSD-P values were calculated for largemouth bass, bluegill, and redear sunfish (Table 5-20). The average PSD and RSD-P for largemouth bass was 90 and 64 respectively. The average bluegill PSD (10) was below the range for a balanced population, as was the average RSD-P (0). Redear sunfish had a summer season PSD of 71 and an RSD-P of 34 and both values were greater then the optimal range for a balanced population. The average bluegill relative weight for the summer sampling period was 100, within the acceptable range (95-100) for the species (Table 5-20). The average relative weight for redear sunfish during the summer sampling was 83, lower then the ideal range for the species. Average largemouth bass relative weights (98) for the summer period were within the recommended range of 95 to 100 reported in the literature. Relative weights for blue catfish (Wr = 109) and channel catfish (Wr = 115) were above the recommended range for the species indicating these fish are in good condition. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 38 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 5-20. Proportional stock density (PSD), Relative stock density (RSD-P) and Relative Weight (Wr) Values for selected species within Falls Tailwater during all three sampling seasons. PSD Species Summer Fall Spring All Seasons Ideal Range Bluegill 10 24 14 14 20 to 60* Largemouth bass 90 83 89 88 40 to 70* Redear sunfish 71 81 80 78 20 to 60* RSD-P Summer Fan S r?n All Seasons Ideal Range Bluegill 0 0 0 0 5 to 20* Largemouth bass 64 53 61 60 10 to 40* Redear sunfish 34 17 22 24 5 to 20* Wr Summer Fan S r?n All Seasons Ideal Range Blue catfish 109 99 107 106 90 to 100* Bluegill 100 94 96 97 95 to 100** Channel catfish 115 95 103 104 90 to 100* Largemouth bass 98 100 98 97 95 to 100** Redear sunfish 83 80 81 81 95 to 100** *(Anderson and Neumann 1996) **(Neumann and Murray 1991) 5.4.2 Falls Tailwater Fall Sample Period A total of 1,186 fish representing 27 species were captured in the Falls tailwater (all gear types combined) between November 4 and November 6, 2003 (Table 5-17). Gizzard shad were the dominant species, comprising 19% of the total catch. Redbreast sunfish, bluegill, white perch, largemouth bass, and redear sunfish each comprised between 7 and 17 % of the fish caught. Shorthead redhorse were more abundant in the tailwater during the fall season, and made up 6 % of the catch. These top seven fish species represented more then 80 % of the fish captured. Electrofishing CPUE in Falls tailwater average 223 fish per hour, with a total of 938 fish collected (Table 5-18). Redbreast sunfish (48.3 per hour) and gizzard shad (42.3 per hour) were the dominant species. Bluegill (33.3 per hour), largemouth bass (21.4 per hour), redear sunfish (20.7 per hour) and shorthead redhorse (11.9 per hour) rounded out the top six species captured by electrofishing. Gillnet CPUE for the Falls tailwater during the fall sampling period was 10.0 fish per net, with a total of 240 fish captured (Table 5-19). White perch were the dominant fish species captured by gillnet with a CPUE of 4.2 fish per net, followed by gizzard shad (1.8 per net), blue catfish (0.8 per net) and shorthead redhorse (0.8 per net). One bluegill was captured by beach seine during the fall sampling period and a total of 6 fish representing 3 species were captured by backpack electrofishing (2 redbreast sunfish, 2 bluegill, 2 tessellated darter). 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 39 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft The average PSD and RSD-P for largemouth bass was 83 and 53 respectively, exceeding the optimal range (Table 5-20). The average bluegill PSD (24) was within the range for a balanced population, whereas the average RSD-P was zero. Redear sunfish had a fall season PSD of 81 and an RSD-P of 17. The PSD value is greater then the optimal range for a balanced population, however the RSD-P value is within the optimal range. The average bluegill relative weight for the fall sampling period was 94, near the acceptable range (95-100) for the species (Table 5-20). The average relative weight for redear sunfish during the fall sampling was 80, lower then the ideal range for the species, indicating a feeding problem. Average largemouth bass relative weight (100) for the fall period was within the recommended range of 95 to 100 reported in the literature and relative weights for blue catfish (Wr = 99) and channel catfish (Wr = 95) were also within the recommended range for the species. 5.4.3 Falls Tailwater Spring Sample Period A total of 886 fish representing 30 species were captured in Falls tailwater (all gear types combined) between May 7 and May 10, 2004 (Table 5-17). The top seven fish captured during the spring sampling included largemouth bass, redbreast sunfish, gizzard shad, bluegill, white perch and threadfin shad and together, they accounted for nearly 70 % of the fish collected. Each of these species made up between 8 and 11 % of the total number of fish captured during the spring. White catfish were more abundant in the tailwater during the spring season than either the summer or fall sampling periods and made up 8 % of the total spring catch. The spring sampling period in Falls yielded the highest species diversity of any of the three seasons sampled. Electrofishing CPUE in the Falls tailwater average 99.1 fish per hour, with a total of 781 fish collected (Table 5-18). Redbreast sunfish (12.8 per hour) and largemouth bass (12.7 per hour) were the dominant species sampled by electrofishing. Gizzard shad (10.3 per hour), bluegill (10.3 per hour), silver redhorse (7.0 per hour), redear sunfish (7.0 per hour) and threadfin shad (6.9 per hour) made up the top seven species sampled. Gillnet CPUE for the spring season was 1.63 fish per net, with 104 fish captured (Table 5-19). The lower spring gillnet CPUE values may be a reflection of the fact that nets were moved frequently and fished for only eight hour sets during the day. No night gillnets were set during the spring sampling period in Falls tailwater and nets were moved frequently in an attempt to sample as much endangered redhorse habitat as possible. Overnight sets were avoided in order to prevent the mortality of any endangered redhorse that may have been captured. There were no fish captured by beach seine in the Falls tailwater during the spring sampling season. Backpack electrofishing yielded one largemouth bass. The average PSD and RSD-P for largemouth bass was 89 and 61 respectively, and both values exceeded the optimum range (Table 5-20). The average bluegill PSD (14) was below the range for a balanced population, as was the average RSD-P (0). Redear sunfish had a spring season PSD of 80 and an RSD-P of 22. The PSD value is greater then the optimal range for a balanced population, however the RSD-P value is near the optimal range. The average bluegill relative weight for the spring sampling period was 96, within the acceptable range (95-100) for the species (Table 5-20). The average relative weight for redear sunfish during the spring sampling was 81, lower then the ideal range for the species. Average largemouth bass relative 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 40 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft weight (98) for the spring period was within the recommended range of 95 to 100 reported in the literature. Relative weights for blue catfish (Wr = 107) and channel catfish (Wr = 103) were both above the recommended range for the species. 5.4.4 Seasonal Differences in Falls Tailwater Although the spring sampling period yielded higher species diversity than either the fall or summer, species composition and CPUE rates were similar for all three sampling periods in Falls tailwater. Largemouth bass, bluegill, redbreast sunfish, white perch, and gizzard shad were consistently among the seven most abundant species sampled for all three seasons. Shorthead redhorse were more abundant in the tailwater area during the fall season then either the spring or summer samplings. The PSD and RSD-P values for largemouth bass, bluegill, and redear sunfish in Falls tailwater were consistent through all three sampling periods. Similar to High Rock and Tuckertown tailwaters largemouth bass within Falls tailwater show high PSD, RSD-P and Wr levels, indicating a population with low or indeterminate mortality of quality-sized fish. Appendix 2 (Figures 1-33 through 1-41) show length frequency distributions for several important game and forage fish found in Falls tailwater. Largemouth bass are successfully spawning as they show a bimodal length frequency distribution with young fish and older spawning age adults. Striped bass, another important game fish in the area, were present in Falls tailwater. These fish are present in Tillery Reservoir due to the current stocking program of the NCWRC. Redear sunfish length frequencies indicate consistent numbers through a variety of size classes suggesting that these fish are successfully reproducing. The low PSD values for bluegill in the Falls tailwater that were suggestive of an unbalanced population dominated by smaller fish, is supported by length frequency data that shows a unimodal distribution of bluegill with a peak in the 10 - 12 cm size classes. Channel and blue catfish length frequencies each show small numbers of quality sized fish present. The three major forage species captured, gizzard shad, threadfin shad and white perch were abundant in the tailwater and are successfully spawning. 5.5 TAILWATER FISH SUMMARY The fish communities sampled in the tailwaters of High Rock, Tuckertown, Narrows and Falls developments were similar, but some differences in species captured are noted. Species diversity recorded during the NAI tailwater assessments ranged from a high of thirty-four species in both High Rock and Falls tailwaters to a low of 29 species recorded in Tuckertown tailwater. Large numbers of bluegill, largemouth bass, gizzard shad and white perch dominated the catches in each tailwater. These four species are among the ten most abundant species captured within each tailwater, comprising 48% of the total catch in High Rock tailwater, 57% in Tuckertown tailwater, 64% in Narrows tailwater and 46% in Falls tailwater. These species are tolerant of the low dissolved oxygen (DO) concentrations often found in the project tailwaters during the summer and given their numbers, they also appear well adapted to the hydro peaking operations that often occur at all four Yadkin Project developments. Black crappies were more abundant in both Tuckertown and High Rock tailwaters than either Narrows or Falls. Common carp and quillback were both in the ten most abundant species sampled in the High Rock tailwater and were either not present or captured in low numbers in the other three tailwaters. The numbers of carp captured in High Rock tailwater were evenly distributed during all three seasons of sampling. Quillback were most abundant in the tailwater during the spring season 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 41 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft and may have been using the tailwater area below High Rock dam for spawning. In the Falls tailwater, silver and shorthead redhorse were in the top ten species collected. The shorthead redhorse was captured at all four tailwaters during the study, but its numbers were lower at the other three tailwaters, where water quality conditions were not as good as those recorded at Falls. The higher catches of shorthead redhorse in the Falls tailwater compared to upstream tailwaters may be due to better habitat and water quality conditions, especially dissolved oxygen levels. The shorthead redhorse (and the black redhorse) are considered to be intolerant to poor water quality, as are some darter species (Scott 1999). Bluegill and largemouth bass maintained consistent relative weights throughout all four tailwaters. Relative weight values were either within or near the ideal ranges for these species in each of the four tailwaters. Average proportional stock density (PSD) and relative stock density (RSD-P) values for largemouth bass were greater then the ideal range within each of the four tailwaters. Bluegill PSD values were within (High Rock and Narrows) or close to (Tuckertown and Falls) the ideal range for the species in all four tailwaters, suggesting a balanced population. However, RSD-P values for bluegill were well below the ideal range for the species in all four tailwaters and this indicated that few large, quality sized fish were available for harvest. Relative weights for black crappie were within or very close to the ideal range in both Narrows and Tuckertown tailwaters, indicating that the fish are in good condition. However, black crappie relative weights in High Rock tailwater were lower than both the ideal range and the average values calculated for the downstream tailwaters, suggesting possible problems finding adequate food sources. The PSD and RSD-P values for black crappie were either within or greater then the ideal range for the species in High Rock, Tuckertown, and Narrows tailwaters, suggesting a balanced population with most size classes represented. Relative weights for redear sunfish in Tuckertown, Narrows and Falls tailwaters were low when compared to the ideal range suggested in the literature for that species suggesting a problem obtaining adequate food sources. The numbers of redear sunfish captured in the High Rock tailwater were too low to assess the population. Channel catfish relative weights were consistent among all four tailwaters and were either within or very close to the ideal range for that species. Striped bass and blueback herring, both anadromous species, are currently present within all of the reservoirs. Striped bass were captured in all four tailwaters during the study, but numbers were low in High Rock (n=11) and Falls tailwaters (n=18), compared to Tuckertown (n=65) and Narrows (n=39). The NCWRC stocks striped bass in all the project reservoirs except Falls (Narrows tailwater). Striped bass captured in the Narrows tailwater (upper Falls reservoir) may have passed downstream through the turbines or spill gates. Those collected in Falls tailwater (upper Tillery Reservoir) may have originated from stockings into Tillery Reservoir or they dropped downstream from Falls Reservoir. Low dissolved oxygen concentrations in southeastern reservoirs have received particular attention in regard to the effects on important game fish such as striped bass. Dissolved oxygen levels below 2 mg/1 and temperatures greater than 25.0 °C constitute unsuitable habitat for striped bass (Coutant and Carroll 1980; Young and Isely 2002). DO conditions of 2 mg/1 and less and water temperatures of greater than 25 °C have been recorded at certain times during the summer months in the High Rock, Tuckertown and Narrows tailwaters(NAI 2005a). Telemetry studies on adult striped bass in southeastern reservoirs have shown that habitat use, distribution and movements are all influenced by seasonal changes in temperature and dissolved oxygen (Schaffler et al. 2002). While long-term 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 42 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft exposure to DO concentrations less then 2 mg/l can be detrimental to individual striped bass, short- term exposure to these conditions are tolerable and don't necessarily lead to high rates of mortality (Jackson and Hightower 2001). Striped bass in Narrows Reservoir (Tuckertown tailwater) are currently the target of cooperative bioenergetic studies by NCWRC and North Carolina State University to evaluate growth in relation to available habitat, particularly the thermal environment. Blueback herring were captured in all four tailwaters during the study with the highest numbers captured in the Tuckertown (n=55) and Narrows (n=61) tailwaters and lesser numbers captured in the Falls (n=11) and High Rock (n=2) tailwaters. The NCWRC stocked blueback herring into Narrows Reservoir during the 1970's and the presence of adult and juvenile sized fish suggests that this population is continuing to maintain itself. Blueback herring captured in both the Narrows (upper Falls reservoir) and Falls (upper Tillery Reservoir) tailwaters may have passed downstream through the turbines or were flushed out of Narrows Reservoir during a spill event. The small numbers of blueback herring captured in High Rock tailwater may be the result of bait-bucket introductions. It should be noted that blueback herring and striped bass are both listed as species of interest in the Restoration Plan for the Diadromous Fishes of the Yadkin-Pee Dee River Basin North Carolina (USFWS 2004). 5.6 FISH SAMPLING DURING LOW AND NORMAL DISSOLVED OXYGEN CONDITIONS One of the objectives of this study was the evaluation of the impacts from existing Project operations on the fish community with regard to the low dissolved oxygen (DO) levels found within the tailwaters during certain times of the year (NAI 2005a). Within the three seasons of fish sampling in the four tailwaters, two events occurred in the Narrows tailwater when both low DO (< 5 mg/1) and normal DO (>5 mg/l)levels were sampled within a 24 hour period. These two events were defined by a change in dissolved oxygen of 2 mg/L or greater within a 24 hour period and in both instances, this change was caused by going from full generation down to no generation. Unit four at Narrows is equipped with air injection valves that increase dissolved oxygen in the tailwater by 2-3 mg/1 when unit four is operated alone (NAI 2005a). The other three units at Narrows do not have air injection. The influence of air injection at unit four on tailwater dissolved oxygen concentrations is diluted as the other units are brought online. The first of the DO comparison sampling events occurred between 2300 hrs on August 30 and 23 00 hrs on August 31, 2003. Dissolved oxygen readings taken in the Narrows tailwater by the field crew during electrofishing sampling ranged from 1.63 to 6.05 mg/1. Figure 5-5 presents the dissolved oxygen and total discharge relationship for the 24 hour time period. Fish sampling at normal dissolved oxygen levels took place within the tailwater from 2300 to 0200 and 1700-1800. Dissolved oxygen concentrations during this period ranged from 5.23 to 6.01 mg/1 during those time periods (Appendix 3). Fish sampling at low dissolved oxygen levels took place during the hours of 1300 and 1500 and dissolved oxygen concentrations during this period were between 1.63 and 1.71 mg/1. Figure 5-5 shows the greatest DO values (6 mg/1) occurred when discharge was 500 cfs and this value decreased to around 3 mg/l when discharge increased to 3,500 cfs. The lowest DO levels (< 2mg/1) in the Narrows tailwater coincided with no discharge during the afternoon hours. When unit four began generating at 500 cfs around 1700 hrs, DO levels rapidly rose to above 6 mg/1. During the summer event in Narrows tailwater, no significant differences (p = 0.5017) were detected in the number of fish species using the tailwater areas during the low and normal DO periods (Figure 5-7). However, 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 43 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Narrows Tailwater -August 30 to September 1, 2003 7 6 5 J a E c 4 X 0 9 3 6 0 2 1 Draft 4000 3500 3000 2500 3 0 2000 0 0 1500 ~ 1000 500 0 0 N (2 V?2 (2 I: W O O N N N O N m V 0 I W O O N (2 V (2 I: of 0 O N N N O N m V 0 I of m O N Hour Figure 5-5. Dissolved oxygen (mg/L) and total outflow (cfs) for 24 hour period during which electrofish sampling in Narrows tailwater (summer 2003). Shaded area denotes 24 hour period of fish sampling. Narrows Tailwater - November 7 and 8, 2003 Figure 5-6 10 6000 -mF- Dissolved Oxygen (nng/L) --A-- Total Outflow (cfs) 9- 5000 8 7 4000 m E 6 U C 3 m o 0 5 3000 a 0 d ? N 4- 0 H N_ 2000 3 2- 1000 1 0 0 o?Nmvu?cormrno?Nm vin co?mrno?Nmo?Nmvu?cormrno?Nmv?n co?mrno?Nm NNNN ? NNNN Hour Dissolved oxygen (mg/L) and total outflow (cfs) for 24 hour period during electrofish sampling in Narrows tailwater (summer 2003). Shaded area denotes 24 hour period of fish sampling. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 44 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Comparison of Catch During Low and Normal DO Periods Summer 2003 - Narrows Blueback Herring Low DO Normal DO Gizzard Shad Low DO Normal DO Threadfin Shad Low DO Normal DO Common Carp Low DO Normal DO White Catfish Low DO Normal DO Channel Catfish Low DO Normal DO Flathead Catfish Low DO Normal DO White Perch Low DO Normal DO Striped Bass Low DO Normal DO Redbreast Sunfish Low DO Normal DO Pumpkinseed Low DO Normal DO Bluegill Low DO Normal DO Largemouth Bass Low DO Normal DO Black Crappie Low DO Normal DO Flat Bullhead Low DO Normal DO Warmouth Low DO Normal DO Blue Catfish Low DO Normal DO Redear Sunfish Low DO Normal DO V IV LV JV YV JV VV /V VV JV IVV IIV ILV IJV Number of Fish Figure 5-7. Species and number of fish of each captured during low and normal dissolved oxygen periods during the 24-hour sampling period in the Narrows tailwater during the summer season. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 45 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft of the eighteen species captured in Narrows tailwater during the 24-hour summer sampling event, fifteen showed fewer individuals present in the tailwater during the low DO period. It is not known if these fish moved away from the tailwater area of if they slowed their movements down making them more likely to avoid capture. The second event took place between 1500 on November 7, 2003 and 1500 on November 8, 2003. Dissolved oxygen concentrations taken in the tailwater during electrofishing sampling ranged from 3.23 to 6.75 mg/L. Figure 5-6 presents the dissolved oxygen and total discharge relationship for the above time period. Fish sampling at normal dissolved oxygen levels took place in the tailwater between the hours of 1800 and 2000. Dissolved oxygen readings from the long term monitors ranged from 6.54 to 6.74 mg/L during those time periods. Fish sampling at low DO levels took place during the hours of 1200 and 1400 with DO's between 3.27 and 3.41. The trend lines in Figure 5-6 show the lowest DO readings (< 4 mg/1) occurred in the afternoon during a no flow (generation off) period, similar to the summer event described above. The highest DO values were recorded when discharge was 500 cfs and this was due to unit four being operated with the air injection system. When discharge was high, DO readings dropped from approximately 8.5 mg/1 to 6.5 mg/1. During the November 24-hour sampling period, the total number of species present during both low and normal DO periods was significantly different (p = 0.0195), with 16 species caught during the normal DO period and 12 species caught during the low DO period.. Of the twenty-one species captured in the tailwater, thirteen had fewer individuals present during the low DO periods (Figure 5-8). Dissolved oxygen readings from the Narrows tailwater continuous monitor, along with generation data for the two periods is presented in Appendix 3. 5.7 FISH STRANDING DURING GENERATION ON/OFF CYCLES Hydroelectric facilities often produce rapid changes of water level as turbines are turned on and off with generation demands. These rapid changes can lead to the stranding of fish that are unable or reluctant to move from habitats that become dewatered. Although generation schedules at all four Yadkin developments had periods of full and non-generation during the tailwater fish sample periods, the stranding of fish was not observed at any of the hydroelectric sites. During this study, drops in tailwater water levels were minor (1 ft or less) at each site after generation went from full or near full generation down to no generation. The lack of stranding at the four tailwaters after flows went from full generation down to no or low generation is because each tailwater is inundated, in part, by the downstream dam. In this sense, the project tailwaters do not operate as a true tailrace (riverine section downstream of the powerhouse) that is found at most hydroelectric projects, but instead become an extension of the downstream reservoir. 5.8 ROBUST AND CAROLINA REMORSE SEARCHES No robust or Carolina redhorse species were captured during the three seasons of fish sampling in the four project tailwaters. The robust redhorse, a Federal species of concern, has recently been collected from the Pee-Dee River, below the Blewett Falls project. The Carolina redhorse is an undescribed sucker species and is also a species of Federal concern. Carolina redhorse individuals have been collected from Blewett Falls Reservoir and the river reach directly below that impoundment. One specimen was also collected in Lake Tillery during November of 2002. NAI efforts, particularly during the spring season, focused on searching the tailwater reaches of the four developments thoroughly for both species. Although searches were conducted in all four tailwaters, sampling 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 46 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Comparison of Catch During Low and Normal DO Periods Fall 2003 - Narrows Blueback Herring Low DO Normal DO Gizzard Shad Low DO Normal DO Threadfin Shad Low DO Normal DO Common Carp Low DO Normal DO Shorthead Redhorse Low DO Normal DO White Catfish Low DO Normal DO Channel Catfish Low DO Normal DO Flathead Catfish Low DO Normal DO White Perch Low DO Normal DO Striped Bass Low DO Normal DO Redbreast Sunfish Low DO Normal DO Pumpkinseed Low DO Normal DO Bluegill Low DO Normal DO Largemouth Bass Low DO Normal DO White Crappie Low DO Normal DO Black Crappie Low DO Normal DO Yellow Perch Low DO Normal DO Bowfin Low DO Normal DO Warmouth Low DO Normal DO Blue Catfish Low DO Normal DO Redear Sunfish Low DO Normal DO 0 10 20 30 40 Number of Fish Figure 5-8. Species and number of fish each captured during low and normal dissolved oxygen periods during the 24-hour sampling period in the Narrows tailwater during the fall season. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 47 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft concentrated on Falls tailwater at the upper end of Lake Tillery. Spring sampling began when water temperatures were in the range of 18-24°C, the temperature thought to trigger spawning in these fish. Gill nets were fished for 8-hour periods and moved frequently in order to cover as much redhorse habitat as possible. Electrofishing focused on areas of woody debris and in cobble/boulder shoal habitat. The search area extended from the base of Falls hydroelectric facility down to the mouth of the Uwharrie River. Despite the intensive surveys, neither redhorse species was found. 6.0 TAILWATER MACROINVERTEBRATE AND MUSSEL ASSESSMENT RESULTS Macroinvertebrate sampling and mussel searches were conducted in the tailwaters of Falls, Narrows, Tuckertown and High Rock Reservoirs during September 2003 (summer sampling), November 2003 (fall sampling), and June 2004 (spring sampling), and the results are briefly summarized in this section. The full report, entitled Benthic Macroinvertebrate Survey Yadkin River, prepared by Pennington and Associates, Inc. (PAI 2005), details the results of this survey and is attached to this report as Appendix 4. 6.1 TRANSECT AND STATION LOCATIONS Figures 6-1 through 6-4 show the locations of mussel search transects within the four tailwaters along with the macroinvertebrate sampling stations located at the quarter points of each transect. Table 6-1 shows the east and west bank endpoints for each transect within the four tailwaters. Within High Rock tailwater, transect 1 was located approximately 500 feet downstream from the dam while transect 2 was located approximately 900 feet downstream. Transect 1 in Tuckertown tailwater was approximately 900 feet downstream of the dam while transect 2 was around 1,300 feet below the dam. The two transects in Narrows tailwater were located approximately 700 and 1,700 feet respectively, below the dam. Transect 1 in Falls tailwater was located approximately 400 feet below the dam while transect 2 was located approximately 1,000 feet below the dam. 6.2 TAILWATER DESCRIPTIONS Habitat (substrate) types along each transect were described during the collection of mussel and macrtoinvertebrate samples. For full descriptions, refer to the PAI report Benthic Macroinvertebrate Survey Yadkin River, attached to this report as Appendix 4 (PAI 2005). Summaries of tailwater habitats from PAI (2005) are provided here. Table 6-2 provides a summary of the physical characteristics of each transect within the four tailwaters. Transect descriptions begin on the right bank (looking downstream) and run across the channel to the left bank. 6.2.1 High Rock Tailwater Figure 6-1 shows the locations of transect 1 and 2 within the High Rock tailwater. Transect 1 was 455 feet long and water depths across this transect ranged from 2 to 10 feet deep. Substrate within transect 1 was comprised mainly of silt covered boulders with lesser amounts of cobble and gravel. Transect 2 was 675 feet long and in water from 3 to 12 feet deep. The first 100 feet of transect 2 were comprised of mud and from 100 to 675 feet across the substrate consisted of silt covered boulders with lesser amounts of cobble and gravel. As seen in Figure 6-1, the power house and associated 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 48 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 6-1. High Rock Macroinvertebrate Stations and Mussel Transects. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 49 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 6-2. Tuckertown Macroinvertebrate Stations and Mussel Transects. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 50 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 6-3. Narrows Macroinvertebrate Stations and Mussel Transects. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 51 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Figure 6-4. Falls Macroinvertebrate Stations and Mussel Transects. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 52 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 6-1. Transect locations for mussel and macroinvertebrate sampling, Yadkin tailwaters 2003. Falls Dam Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35023'45.4" W80004'22.5" N35023'36.7" W80004'28.1" 2 N35°23'36.2" W80°04' 15.9" N35°23'31.2" W80°04'21.3" Badin Dam (The Narrows) T ailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°25'06.6" W80°05'28.0" N35°25'04.5" W80°05'30.6" 2 N35°24'54.6" W80°05'22.8" N35°24'54.4" W80°05'27.3" Tuckertown Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°29'05.2" W80°10'23.2" N35°28'58.3" W80°10'38.5" 2 N35°29'02.2" W80°10'18.6" N35°28'55.1" W80°10'24.5" High Rock Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°35'58.5" W80°13'59.1" N35°35'55.4" W80°14'04.8" 2 N35°35'55.3" W80°13'57.2" N35°35'52.0" W80°14'03.8" Table 6-2. Physical characteristics of mu ssel/m acroinverteb rate sampling transects within the four project tailwaters. Falls Dam Narrows Dam Tuckertown High Rock Distance from Dam TI (ft) -400 -700 -900 -500 Width (11) T1 976 -300 843 455 Distance from Dam T2 (ft) 1000 1,700 1,300 -900 Width (11) T2 671 -300 1,188 675 Depth (ft) T1 4-20 4-20 2-8 2-10 Depth (ft) T2 4-8 4-10 2-12 3-12 0'-200' bedrock Boulders and boulders ' ' Boulders, few Substrate TI , cobble and 200 -300 Bedrock, cobble and gravel Boulders with boulders, silt gravel covered cobble, gravel with silt and silt 0-135' mud on bedrock 135'- 0-100' mud Boulders Boulders, cobble 250' boulders ' ' 100'-675' Substrate T2 , cobble and with lesser 500 -750 boulders few gravel amounts of gravel boulders few ' cobble and and silt cobble 750 - gravel covered 1188' bedrock, with silt cobble with silt 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 53 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft flow of water are located on the right side of the dam, looking upstream. A large field of boulders and slower water is located on the left side of the tailwater in the area below the spill gates. 6.2.2 Tuckertown Tailwater Figure 6-2 shows the locations of transect 1 and 2 within the Tuckertown tailwater. Transect 1 was 843 feet wide and water depths along it ranged from 2 to 8 feet. Substrate along transect 1 consisted of bedrock, boulder and silt. Transect 2 was 1,188 feet long and depths ranged from 2 to 12 feet along the length of the transect. The substrate of transect 2 from 0 to 135 feet consisted of mud overlying bedrock, while boulders were the dominate substrate from 135 to 250 feet. A mixture of silt covered bedrock, boulder, and cobble comprised the rest of transect 2. As shown in Figure 6-2 and similar to High Rock tailwater, the power house and the associated water flow are located on the right side of the tailwater. A large field of boulders and slower moving water are located on the left side of the tailwater in the area below the spill gates. 6.2.3 Narrows Tailwater Figure 6-3 shows the locations of transects 1 and 2 within the Narrows tailwater. Transect 1 was approximately 300 feet long and along its length, water depths ranged from 4 to 20 feet deep. Bottom substrate along transect 1 was characterized as bedrock and boulders from 0 to 200 feet and as boulder and cobble with lesser amounts of gravel and silt from 200 to 300 feet. Transect 2 was approximately 300 feet long with water depths ranging from 4 to 10 feet. Water depth along transect 1 was approximately 4 ft deep for most of its length, except for a deeper zone along the downstream looking right bank. Substrate along transect 2 was comprised of boulder and cobble with lesser amounts of gravel and silt. As shown in Figure 6-3, the turbine discharge is located along the right hand bank, above transect 1. The tailwater channel is very narrow until the area below transect 2, where the tailwater widens. A shallow overflow channel is located along the right side of the tailwater and rejoins the mainstem of the tailwater in the area below transect 1. 6.2.4 Falls Tailwater Figure 6-4 shows the locations of transect 1 and 2 within the Falls tailwater. Transect 1 was 976 feet long and water depths along it ranged from 4 to 20 feet. The bottom substrate was comprised mostly of boulders and cobble with mixed lenses of gravel. Transect 2 was 671 feet long with water depths that ranged between 4 and 8 feet. Bottom substrate along transect 2 was similar to that of transect 1. As shown in Figure 6-4, the endpoints of transect 2 were located on two islands on either side of the main channel within the Falls tailwater. The main flow from the turbines is located on the left side of the dam. A large area of boulders is located on the right side of the upper tailwater in the area below the spill gates. 6.3 MUSSEL SEARCHES A total of seven species of freshwater mussels were found within the four tailwaters. Table 6-3, taken from PAI (2005) provides a summary of mollusca species found within the four tailwaters. Falls tailwater had the greatest mussel diversity with seven species and 575 total individuals. In Falls tailwater, Elliptio complanta (Eastern Elliptio) was the most abundant (57%) mussel species, while Elliptio cf lanceolata (Pee Dee Lance)(20%) and Lampsilis radiata (Eastern lamp mussel)(20%) 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 54 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Table 6-3. Summary of Mollusca taken from Yadkin River, 2003-2004. Species Falls Dam Tailwater Badin Dam (Narrows) Tailwater Tuckertown Tailwater High Rock Tailwater Anodonta implicata R 1 Alewife floater Elliptio complanta 328 16 Eastern Elliptio Elliptio cf lanceolata 113 1 Pee Dee Lance Lam silis radiata 117 R Eastern lam mussel P anodon cataracte 1 2 Eastern floater Utterbackia imbecillis 8 2 4 1 Paper and shell Villosa delumbis 8 Eastern creekshell Total No. Of Unionidae Species 7 6 1 1 Total No. Of Individuals 575 22 4 1 Corbicula fluminea A A A A Cipangopalucdinea chinensis 231 Chinese mystery snail • R = represented by relics only • A = abundant were common. Narrows tailwater had 6 species with 22 total individuals. Elliptio complanta (73%) was the most abundant species within the Narrows tailwater. Elliptio complanata was present in both the Falls and Narrows tailwaters. One specimen ofAnodonta implicata (Alewife floater) was found within the Narrows tailwater. The only mussel species found in the Tuckertown and High Rock tailwaters was the Utterbackia imbecillis (Paper pond shell) with four individuals found in Tuckertown and one in High Rock. Corbicula fluminea, the Asiatic clam, is an invasive species that was abundant throughout all four tailwaters. For a detailed listing of mussels found within each tailwater by season, refer to Tables IA, 2A, and 3A in PAI (2005). In addition to inventorying the freshwater mussel species present in the four tailwaters, the presence of any rare, threatened or endangered mussel species was to be noted. There were no federally endangered mussel species found within any of the four tailwaters. Elliptio cf lancolata (PeeDee Lance) is listed as endangered by the state of North Carolina. This species was found in the tailwaters of both Falls and Narrows. Two species, Anodonta implicata (alewife floater) and Lampsilis radiata (Eastern lamp mussel), are both listed as threatened by the state of North Carolina. Anodonta implicata was found in both Falls (relic shells only) and Narrows tailwaters. Lampsilis radiata was found in Falls and Narrows (relics only) tailwaters. Villosa delumbis (Eastern creekshell) is considered significantly rare by the North Carolina Heritage Program and 8 individuals were found within the Falls tailwater. In High Rock Reservoir, mussel species were found and identified by state personnel during the severe drought of 2002. Three species, Elliptio complanata (Eastern Elliptio), Anodonta implicate (Alewife floater), and Corbicula fluminea (Asiatic clam) were identified from the shell samples 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 55 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft collected in the main body of High Rock. The mussels were collected by Peter Diamond, of the North Carolina Zoological Park and were identified by Sarah McRae, freshwater ecologist with the North Carolina Natural Heritage Program. 6.4 MACROINVERTEBRATES There were 6 phyla, 24 orders, and 41 families represented by the 99 benthic macroinvertebrates species found in the four tailwaters. Table 6-4, taken from PAI (2005) provides a list of the macroinvertebrates found within the four tailwaters. The spring sampling in Falls tailwater yielded the highest number of species with 53 found and the summer sampling in High Rock yielded the lowest number of species collected with 29. The spring sampling in Narrows (12,008/12m2) and Falls (10,172/12m2) yielded the highest densities of individuals. The lowest numbers of individuals per sample were recorded in Falls (1,420/12m2) and Narrows (1,333/12m2) during the fall sampling. Table 6-5 (taken from PAI macroinvertebrate survey report) presents the percent composition of the most abundant benthic macroinvertebrate species within each of the four tailwaters during the three seasons of sampling. Dominant species in Falls tailwater during the three sampling periods included Corbicula fluminea (Asiatic clam; summer and fall) and Caecidota sp. (isopod sp.; spring). The three sampling periods in Narrows were dominanted by Rheotanytarsus sp. (midge sp.; summer), Corbicula fluminea (fall), and Caecidotea sp. (June). Tuckertown samplings were dominated by Musculium transversum (Fingernail clam; summer and fall) and Caecidotea sp. in the spring. Musculium transversum was the dominant species in High Rock during the summer and spring while Caecidotea sp. was dominant in the fall. The EPT index is the total number of species found in an area within the pollution sensitive groups Ephemeroptera, Plecoptera, and Trichoptera and is considered a measure of water quality. As the EPT value increases, water quality will tend to do the same. Table 6-6 shows the EPT Index score for standard collecting effort within the piedmont area of North Carolina. The Hilsenhoff Biotic Index uses arthropod populations to evaluate water quality. Species are assigned pollution tolerance values from 0 to 10. A value of 0 would be assigned to a species found only in unaltered streams with high water quality whereas a value of 10 would be assigned to a species know to occur in severely polluted areas. Table 6-6 presents the range of Hilsenhoff Biotic Index values and the associated water quality classifications for the piedmont area of North Carolina. The EPT and Hilsenhoff Biotic Index values indicated that the benthic fauna in Falls tailwater exists under mostly "poor" water quality conditions during the spring sampling and no better than "fair" during the summer and fall sampling periods. Benthic fauna in Narrows tailwater follows the same pattern of "poor" water quality conditions during the spring period with "fair" water quality conditions during the summer and fall sampling periods. Water quality conditions for the benthic fauna of High Rock and Tuckertown tailwaters was determined to be "poor" for all three sampling periods based on the results of the EPT and Hilsenhoff Biotic Index. For seasonal EPT Index and Hilsenhoff Biotic Index scores for all four tailwaters, refer to Table 6-4. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 56 Normandeau Associates, inc. v, J Z O O Q fD n y y O n fD y O n Table 6-4. Benthic Macroinverteb rates collected from Yadkin River 2003-2004 (No./-2m2) Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock SPECIES T.V. F.F.G. Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL COELENTERATA H drozoa H droida Hydridae Hydra sp. 1 PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii 1 2 12 Dugesia tigrina 7.2 117 294 245 685 75 50 608 717 513 250 29 150 NEMATODA 1 10 2 1 MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata 5.1 FC 1 2 1 5 Utterbackia imbecillis 1 1 1 Veneroida Corbiculidae Corbicula fluminea 6.1 FC 542 207 18 3 682 580 205 28 1118 1122 64 119 Sphaeriidae -8 FC 13 5 Eupera cubensis 5.7 FC 114 2 44 5 58 Musculium transversum -8 FC 307 306 2461 3921 89 72 5239 1610 623 165 349 1406 Pisidium sp. 6.5 FC 6 1 Gastro oda Meso astro oda Hydrobiidae -8 SC Amnicola limosa 4 6 49 4 10 30 5 106 17 Viviparidae Cipangopaludina chinensis 2 12 Basommatophora Ancylidae SC Ferrissia rivularis 6.6 SC 49 19 198 2 33 14 26 8 9 2 f?D 1 y Qo n C?O 01 n y y y y fD O rz) (continued) to Oc Z O O Q fD n y y O n Ci tq n Table 6-4. (Continued) Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock SPECIES T.V. F.F.G. Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Physidae Physella sp. 8.8 CG 12 35 20 17 27 12 42 43 839 404 35 58 Planorbidae -6 SC Helisoma anceps 6.2 SC 1 15 1 Menetus dilatatus 8.2 SC 2 24 409 62 2 1 11 123 227 163 26 180 ANNELIDA Oli ochaeta .1 CG Tubificida Naididae -8 CG 14 19 194 53 8 72 Dero sp. 10.0 CG 3 3 37 3 10 80 1 271 31 Nais sp. 8.9 CG 1 4 Nais communis 8.8 CG 31 24 43 51 76 180 2 25 Nais bretscheri ?6 CG 4 Pristina sp. 9.6 CG 1 Pristina leidyi 9.6 CG 2 Pristinella sp. 7.7 CG 1 Ripistes parasita 2 FC 90 87 2 1 Slavina appendiculata 7.1 CG 3 193 1758 25 364 Spirosperma sp. 5.3 CG 1 Stylarza lacustris 9.4 CG 3 16 156 403 Tubificidae w.o.h.c. 7.1 CG 2 7 98 30 11 22 7 3 266 321 60 23 Limnodrzlus hoffineisteri 9.5 CG 116 16 Tubificidae w.h.c. 7.1 CG 6 1 40 1 8 Branchiura sowerbyi 8.3 CG 47 57 Quistadrzlus multisetosus 3.9 CG 2 Lumbriculida Lumbriculidae 7.0 CG 37 9 1 33 17 1 526 1197 8 Hirudinea -8 P 4 46 13 2 81 2 13 26 27 8 Erpobdellidae -8 P 1 2 8 14 5 6 20 25 6 Erpobdella punctata P 2 Rh nchobdellida Glossiphoniidae -8 P 6 20 1 4 27 10 Batrachobdella phalerata 7.6 P 1 Helobdella sp. -6 P 9 70 5 Helobdella stagnalis 8.6 P 72 41 53 4 52 149 f?D 1 y Qo n 01 C? O01 n y y fD y y fD (continued) v, Z O O Q fD n y y O n Ci f?D tq n Table 6-4. (Continued) Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock SPECIES T.V. F.F.G. Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Helobdella triserialis 9.2 P 1 2 27 4 5 40 24 36 115 Placobdella translucens 9.0 P 2 ARTHROPODA Crustacea Ostracoda 5 5 1 1 Candoniidae Candona sp. 4 Cladocera Daphnidae Daphnia sp. 1 Sididae Sida crystillina 15 1 20 9 9 2 Co e oda 5 1 1 C clo oida 5 10 Iso oda Asellidae -8 SH Caecidotea sp. 9.1 CG 225 542 646 1130 97 238 1356 1641 1762 1952 557 272 Am hi oda Crangonyctidae Crangonyx sp. 7.9 CG 1 12 1 1 2 Talitridae Hyalella azteca 7.8 CG 70 123 22 158 93 295 1 826 813 11 7 Decapoda Cambaridae 7.5 1 2 Insecta Collembola 1 E hemero tera Caenidae -7 CG Caenis sp. 7.4 CG 1 3 11 1 4 58 10 5 Heptageniidae -4 SC Stenacron interpunctatum 6.9 SC 4 1 9 Stenonema sp. -4 SC 1 1 Tricorythidae Tricorythodes sp. 5.1 CG 1 (continued) f?D 1 y Qo n c 01 C? O01 n y y fD y y fD rz) 0 Z O O Q fD n y O n ai tq n Table 6-4. (Continued) Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock SPECIES T.V. F.F.G. Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Odonata Coenagrionidae .9 P Argia sp. 8.2 P 2 1 1 Corduliidae .5 P Neurocordulia sp. 5.0 1 Gomphidae Gomphus sp. 5.8 P 1 Neuro tera Sisyridae 1 Tricho tera Hydropsychidae -4 FC Cheumatopsyche sp. 6.2 FC 68 146 1 Hydroptilidae -4 PI 1 Hydroptila sp. 6.2 PI 10 12 9 1 11 30 12 1 Leptoceridae CG Ceraclea sp. 2 CG 1 Polycentropodidae ?6 FC Cyrnellus fraternus -8 FC 134 231 32 7 55 13 47 38 27 Neureclipsis sp. 4.2 FC 1 Di tera 40 Chaboridae Chaoborus punctipennis 8.5 P 8 10 19 Chironomidae 35 62 3 211 124 13 49 Ablabesmyia mallochi 7.2 P 20 1 Ablabesmyia rhamphe gp. -6 P 50 44 17 2 5 4 438 225 1 14 Chironomus sp. 9.6 CG 14 15 2 19 Conotanypus sp. P 1 Coelotanypus sp. 8 P 36 Conchapelopia sp. 8.4 P 1 Cricotopus sp. -7 CG 157 52 116 376 1806 2 Cricotopus bicinctus 8.5 CG 61 6 5 2 9 460 243 9 7 Cryptochironomus sp. 6.4 P 1 2 Diamesa sp. 8.0 CG 1 Dicrotendipes lucifer 8.0 CG 1 29 41 17 31 Dicrotendipes neomodestus 8.1 CG 12 4 5 1 10 216 166 3 f?D 1 y Qo n C? O01 n y y fD y y fD (continued) Z O O Q fD n y O n Ci y O n Table 6-4. (Continued) Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock SPECIES T.V. F.F.G. Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Sep-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Nov-03 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Jun-04 TOTAL Dicrotendipes simpsoni 10.0 CG 10 55 1579 1999 3 21 393 669 6 36 17 122 Dicrotendipes sp. 8.1 CG 7 3 8 Endochironomus sp. SH 1 1 1 Einfeldia natchitocheae 108 Eukiefferiella claripennis gp. 5.6 CG 1 Glyptotendipes sp. 9.1 FC 3 24 220 576 1 16 912 276 14 35 97 367 Microtendipes pedellus gp. 5.5 CG 20 5 Nanocladius distinctus 7.1 CG 6 28 40 271 6 12 107 5 22 3 5 Orthocladius (Euorthocladius) sp. -4 CG 2 25 1 1 Parachironomus sp. 9.4 CG 1 16 30 59 110 18 133 165 3 214 Phaenopsectra sp. 6.5 SC 3 Parakiefferiella sp. 5.4 CG 1 2 1 Polypedilum flavum 4.9 SH 2 5 Polypedilum illinoense 9.0 SH 5 Procladius sp. 9.1 P 1 1 2 10 Pseudochironomus sp. 5.4 CG 5 3 20 Rheotanytarsus sp. 5.9 FC 137 715 10 13 1 15 77 20 Stenochironomus sp. 6.5 SH 1 5 Tanytarsus sp. 9.2 FC 1 177 35 39 9 Tribelos fuscicorne 6.3 CG 1 316 Tribelos sp. 6.3 CG 1 Tvetenia bavarica gp. 3.7 CG 1 Xenochironomus xenolabis 7.1 P 4 27 21 5 2 8 20 18 5 Empididae 7.6 P Hemerodromia sp. -6 P 2 1 Simuliidae -6 FC Simulium sp. 6.0 FC 2 Tipulidae Tipula sp. 7.3 SH 1 TOTAL NO. OF ORGANISMS 2030 3179 6445 8981 1420 1333 9856 5695 10172 12008 1878 4000 TOTAL NO. OF TAXA 42 48 38 29 34 33 39 42 53 37 44 46 EPT INDEX 6 5 2 2 4 3 2 5 5 2 1 NC BIOTIC INDEX 7.14 7.33 8.54 8.57 6.94 7.19 8.20 8.38 8.09 7.75 8.65 8.24 f?D 1 y Qo n 01 C? O?1 n y y y y fD O Tailwater Fish & Aquatic Biota Assessment Draft Table 6-5. Percent composition of the dominant benthic macroinvertebrate species by sampling season. September 2003 November 2003 June 2004 Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High SPECIES Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Dugesia tigrina 9.3 7.6 6.2 12.5 Corbicula fluminea 26.7 48.0 43.5 11.0 9.3 Musculium transversum 15.1 9.6 38.2 43.7 6.2 53.2 28.3 18.6 35.2 Physella sp. 8.2 Menetus dilatatus 6.3 Dero sp. 14.1 Slavina appendiculata 14.6 9.1 Lumbriculidae 10.0 Caecidotea sp. 11.1 17.0 10.0 12.6 6.8 17.9 13.8 28.8 17.3 16.3 29.7 6.8 Hyalella azteca 11.1 8.2 6.8 Cyrnellus fraternus 7.3 Cricotopus sp. 15.0 Dicrotendipes simpsoni 24.5 22.3 11.7 Glyptotendipes sp. 9.2 9.2 Rheotanytarsus sp. 22.5 Table 6-6. EPT Index and Hilsenhoff Biotic Index scores along with associated water quality for Piedmont area of North Carolina. Bioclassification NC Piedmont EPT Value NC Piedmont Biotic Index Excellent >31 <5.19 Good 24-31 5.19-5.78 Good-Fair 16-23 5.79-6.48 Fair 8-15 6.49-7.48 Poor 0-7 >7.48 7.0 IMPACTS OF EXISTING PROJECT OPERATIONS ON FISH AND AQUATIC BIOTA IN THE TAILWATERS One of the objectives of this study was to consider impacts from Project operations on aquatic biota in the Project tailwaters. Two types of impacts were considered potentially significant at the Yadkin Project; the effects of low tailwater dissolved oxygen conditions, and the effects of project peaking operations. Of these two, based on the aquatic biota surveys conducted in the Yadkin Project tailwaters it appears that the greatest impact of project operations to fish and other aquatic biota existing in the four Yadkin tailwaters is the low DO conditions that can occur in all four tailwaters. The low dissolved concentrations in the project tailwaters result from project operations. At each dam, both surface and bottom water from the upstream reservoir is entrained and mixed during passage through the turbines, which can cause lower dissolved oxygen concentrations in the tailwaters if the bottom water is oxygen depleted. Low dissolved oxygen concentrations can also occur when tailwater flows are reduced to near zero for a prolonged period. Peaking impacts can also occur at hydropower projects and can affect fish communities by interrupting flows, feeding cycles, spawning and causing rapid changes in water quality, including 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 62 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft DO and water temperatures in the tailwater areas. Fluctuating water levels in tailwaters due to hydro peaking operations can also impact aquatic biota by exposing habitat and in some cases stranding aquatic life when generation goes from high generation with subsequent high flows down to minimal or no generation in a short time period. The occurrence of low DO levels in the Yadkin project tailwaters and the causes for these conditions are reported in detail in Normandeau's Yadkin Water Quality Report (NAI 2005a). For example,at the High Rock tailwater during 2004, an average-to-wet water year, average daily dissolved oxygen concentrations were below 5mg/1 on 107 days (see NAI 2005a, Table 2.4-3). In Tuckertown tailwater in 2004, average daily DO concentrations were below 5 mg/1 standards on 96 days and in Narrows, average daily DO concentrations were below 5 mg/1 on 75 days. At Falls, there is a significant improvement in water quality and average daily DO concentrations fell below 5 mg/1 on only 4 dates in 2004. One of the primary impacts of project operations on macroinvertebrates in the tailwaters is caused by low DO levels. Macroinvertebrate rankings were poor for all seasons in High Rock and Tuckertown tailwaters, and this is related to the marginal water quality found at these two sites. At Narrows and Falls tailwaters, the macroinvertebrate communities ranked poor in the spring but fair during the summer and fall sample periods and this is likely due to the better water quality found in the tailwaters of the two lower developments. Similar water quality impacts were also evident for the mussel species. At High Rock and Tuckertown, only one mussel species was collected during all three sampling periods. At Narrows, six mussel species were collected (22 individuals) and at Falls, seven mussel species (575 individuals) were found. Falls tailwater has the most habitat available for mussels, but it also has the best water quality of the four Yadkin Project tailwaters. Four RTE mussel species were found during the surveys, including the NC endangered species Elliptio lancolata (PeeDee lance), which was found in Falls and Narrows tailwaters. Two NC threatened mussel species were found, Anodonta implicata (alewife floater) and Lampsilis radiate (eastern lamp mussel). The alewife floater was found in Falls and Narrows and the eastern lamp mussel was also found in Falls and Narrows, but only relic shells were found of this species. Another mussel species listed as significantly rare by the NC Heritage Program, Villosa delumbis was found in the Falls tailwater (8 individuals). During the tailwater fish collections in 2003 and 2004, the differences in fish catches during normal DO levels (5 mg/1 or greater) and low DO periods (at least a 2mg/1 drop from normal) over two 24 hour periods were analyzed (see Section 5.5). The first test occurred during the summer collections at Narrows and of the 18 fish species collected, 15 had fewer individuals captured during the low DO period. In the second test that occurred during the fall sampling at Narrows, there were significantly fewer species (P=0.019) captured during the low DO period, and of the 21 fish species, 17 had fewer individuals collected during the low DO period. It is not known if the fish ceased or slowed their movements during the low DO tests making them less available for capture or moved out of the tailwater area. Low dissolved oxygen levels and the effects of minimum flows in the tailraces of two TVA hydro projects (Douglas and Cherokee) were studied between 1987 and 1996, including what improvements to the fish communities could be realized by providing minimum flows and by reaerating the turbine discharge to improve DO levels (Scott 1999). It was reported that just providing minimum flows did not substantially improve the fish community overtime at Cherokee (1988-1995) and the reason may 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 63 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft have been the low DO's recorded (<4 mg/1) at the project for 100 days or more each year. However, when reaeration equipment was put in place at the Douglas development to bring the DO concentrations up to 4 mg/1, steady improvement in the fish community was documented between 1988 and 1996 (Scott 1999). The fish populations currently in the four Yadkin tailwaters have been shaped by the current project operations, including peaking flows and low DO concentrations that occur in three of the four tailwaters (all but Falls) between 20 and 29% of the year during a normal year. Many of the fish species present are tolerant of marginal water quality, such as gizzard shad, white perch and largemouth bass and this is why these species dominate the catches at Yadkin. Fish species that cannot tolerate marginal water quality (especially low DO), such as some of the darter and minnow species are generally absent from the catches. Relative weights (Wr) are an indication of a fish's condition, and if these values fall below a range reported for the species, it can indicate a problem with feeding. Largemouth bass either exceeded or fell within the ideal range for Wr in all four tailwaters, indicating they are having no problem securing food. They also are successfully spawning in all four tailwaters (or the reservoir backing up to the tailwaters), given the various size classes captured. Many large sized fish are available for capture, suggesting that fishermen are releasing many of the quality fish. Largemouth bass and some other predators frequently do well in reservoirs with fluctuating water levels (see NAI 2005b) because they take advantage of young forage fish drawn from their cover when lake elevations drop. Black crappies captured in the High Rock tailwater during all three seasons had low relative weights, suggesting that this species may be having a problem securing enough food. These fish did not have a balanced population (PSD value slightly below ideal range), but they are successfully spawning. It is not clear if the problems with feeding are related to the marginal water quality. Relative weights for black crappies captured downstream in Tuckertown and Narrows tailwaters were either within or just below the recommended range for this species and their populations were balanced (PSD values in range). Impacts to tailwater fish populations due to stranding are minor at all four tailwaters because the tailwater areas remain watered up when generation is shut down and discharge flow ceases. Tailwater water surface elevations do fluctuate 1 or 2 ft, but no pools or sections of the tailwater were isolated - there is connectivity to all areas of the four Project tailwaters when generation ceases. No rare, threatened or endangered (RTE) fish were captured during the three seasons of sampling the project tailwaters. Habitat that the robust redhorse and Carolina redhorse are known to frequent were intensively sampled with gill nets and electrofishing gear, especially during the spring spawning season, but none were found in the project tailwaters. 8.0 CITED REFERENCES Alcoa Power Generating, Inc. (APGI) Yadkin Division. 2002. Yadkin Hydroelectric Project FERC No. 2197-NC. Initial Consultation Document. Anderson, R.O. 1980. Proportional stock density (PSD) and relative weight (Wr): interpretive indices for fish populations and communities. Pages 27 - 33 in S. Gloss and B. Shupp, editors. Practical fisheries management: doing more with less in the 1980's. Workshop proceedings, New York Chapter, American Fisheries Society, Ithaca, New York, USA. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 64 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Anderson, R.O. and R.M. Neumann. 1996. Length, weight, and associated structural indices. Pages 447-482 in B. R. Murphy and D. W. Willis, editors. Fisheries Techniques, 2 d edition. American Fisheries Society, Bethesda, MD. Anderson, R.O. and A. S. Weithman. 1978. The concept of balance for coolwater fish populations. Pages 371-381 in R.L. Kendall, editor. Selected coolwater fishes of North America: proceedings of a symposium held in St. Paul, Minnesota March 7-9, 1978. Special Publication; no. 11. American Fisheries Society, Washington DC. Bister, T.J., D.W. Willis, M.L. Brown, S.M. Jordan, R.M. Neumann, M.C. Quist, and C.S. Guy. 2000. Proposed standard weight (Ws) equations and standard length categories for 18 warmwater nongame and riverine fish species. North American Journal of Fisheries Management. 20: 570-574. Coutant, C.C., and D.S. Carrol. 1980. Temperatures occupied by ten ultrasonic-tagged striped bass in freshwater lakes. Transactions of the American Fisheries Society. 109: 195-202. Etmer, D. A. and W.C. Starnes. 1993. The fishes of Tennessee. University of Tennessee Press, Knoxville, TN. Gabelhouse, D.W. 1984. A length-categorization system to assess fish stocks. North American Journal of Fisheries Management. 4:273-285. Jackson, J.R. and J.E. Hightower. 2001. Reservoir striped bass movements and site fidelity in relation to seasonal patterns in habitat quality. North American Journal of Fisheries Management. 21:34-45. Muoneke, M.I. and K.L. Pope. 1999. Development and evaluation of a standard weight (Ws) equation for blue catfish. North American Journal of Fisheries Management. 19: 878-879. Murphy, BR., D.W. Willis, T.A. Springer. 1991. The relative weight index in fisheries management: status and needs. Fisheries. 16(2): 30-38. Neumann, R.M. and B.R. Murphy. 1991. Evaluation of the relative weight (Wr) index for assessment of white crappie and black crappie populations. North American Journal of Fisheries Management. 11: 543-555. Normandeau Associates, Inc. 2005a. Draft Yadkin Water Quality Report. Prepared for Alcoa Power Generating, Inc. Yadkin Division. Normandeau Associates, Inc. 2005b. Draft Yadkin Reservoir Fish and Aquatic Habitat Assessment. Prepared for Alcoa Power Generating Inc. Yadkin Division. Pennington and Associates, Inc, 2005. Benthic Macroinvertebrate Survey Yadkin River. Produced for Alcoa Power Generating Inc. Yadkin Division. Robust Redhorse Conservation Committee. 2003. Intensive Surveys for the Robust Redhorse (Moxostoma robustum) on the Pee Dee River below Blewett Hydroelectric Plant, 2003 Work Plan. Schaffler, J.J., J.J. Isely and W.E. Hayes. 2002. Habitat use by striped bass in relation to seasonal changes in water quality in a southern reservoir. Transactions of the American Fisheries Society. 131:817-827. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 65 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Scott, E. M. 1999. Tailwater fish index (TFI) development for Tennessee river tributary tailwaters. Pages 507 - 522 in T. P. Simon, editor. Assessing the Sustainability and Biological Integrity of Water Resources Using Fish Communities. CRC Press LLC, Boca Raton, FL. USFWS, NMFS, NCWRC. 2004. Draft Restoration Plan for the Diadromous Fishes of the Yadkin- PeeDee River Basin North Carolina. Young, S.P. and J.J. Isely. 2002. Striped bass annual site fidelity and habitat utilization in J. Strom Thurmond reservoir, South Carolina - Georgia. Transactions of the American Fisheries Society. 131:828-837 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 66 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft APPENDIX 1 Tailwater Fish and Aquatic Biota Assessment Final Study Plan June, 2003 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Yadkin Project (FERC No. 2197) Tailwater Fish and Aquatic Biota Assessment Final Study Plan June, 2003 Background Alcoa Power Generating Inc. (APGI) is the licensee for the Yadkin Hydroelectric Project. The Yadkin Project is currently licensed by the Federal Energy Regulatory Commission (FERC) as Project No. 2197 This license expires in 2008 and APGI must file a new license application with FERC on or before April 30, 2006 to continue operation of the Project. The Yadkin Project consists of four reservoirs, dams, and powerhouses (High Rock, Tuckertown, Narrows, and Falls) located on a 38-mile stretch of the Yadkin River in central North Carolina. The Project generates electricity to support the power needs of Alcoa's Badin Works, to support its other aluminum operations, or is sold on the open market. As part of the relicensing process, APGI prepared and distributed, in September 2002, an Initial Consultation Document (ICD), which provides a general overview of the Project. Agencies, municipalities, non-governmental organizations and members of the public were given an opportunity to review the ICD and identify information and studies that are needed to address relicensing issues. To further assist in the identification of issues and data/study needs, APGI has formed several Issue Advisory Groups (IAGs) to advise APGI on resource issues throughout the relicensing process. IAGs will also have the opportunity to review and comment on Draft Study Plans. This Draft Study Plan has been developed in response to comments on the ICD and through discussions with the Fish and Aquatics JAG, to provide additional necessary information for consideration in the relicensing process. Issues The following issues were raised during initial consultation regarding tailwater fish and aquatic biota at the Yadkin Project: ¦ Effects of Yadkin Project reservoir releases on tailwater fish, macroinvertebrates and aquatic habitat ¦ Current status of rare, threatened and endangered (RTE) aquatic species at the Yadkin Project that could be impacted by Project operations Objectives On March 12, 2003 the Fish and Aquatics JAG met and discussed objectives for the tailwater fish and aquatic biota study. Over the course of those discussions the following objectives were identified for the study. Describe tailwater habitats in all four Yadkin development tailwater areas. Inventory and assess the resident fish community in the Project tailwaters on a seasonal basis (spring, summer & fall) to develop baseline data that can be used to detect changes over time. Evaluate the impacts of existing Project operations on the tailwater fish community, such as impacts associated with generation schedules (generation on/off), and impacts due to the low dissolved oxygen (DO) found in the tailwaters during certain times of the year. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft ¦ Inventory and assess the macroinvertebrate and mussel species in the Project tailwaters on a seasonal basis to develop baseline data that can be used to detect changes in these communities overtime. Evaluate impacts of existing Project operations on the tailwater macroinvertebrate community and describe tailwater habitats. ¦ Search for RTE mussel species in Project tailwaters. ¦ Search for RTE fish species, including the Robust and Carolina Redhorse species, in the Project tailwaters during the spring (spawning period) and during the summer and fall fish surveys. Following the March 12, 2003 IAG meeting, Normandeau prepared a draft study plan for the Yadkin Project Tailwater Fish and Aquatic Biota Assessment. This draft study plan was distributed electronically to the Fish and Aquatics IAG on April 4, 2003 for review prior to the next IAG meeting scheduled for April 9, 2003 in Badin, NC. Comments on the Draft Tailwater Fish and Aquatic Biota Assessment at the meeting included discussions on modifying the fish sampling plan in the Project tailwaters so that sampling would occur during generation and during no generation periods to see if it effects fish movement in and out of the tailwaters. It was also requested that more detail be provided in the study plan, including particulars on the sampling design and other parameters that will be collected during tailwater fish surveys, such as water quality sampling. Discussions also focused on sampling in the tailwaters during low DO and normal DO periods each season. It was requested that the extent of the low DO "plume" be determined, as well as its impacts to fish movements into and out of the tailwaters. Other comments included determining the change in the amount and quality of habitat in the tailwaters between peaking and non-peaking operations, especially if there is stranding due to fluctuating tailwater elevations. It was requested that the mussel searches in the tailwaters be expanded beyond the two transects proposed for each tailwater so that good mussel habitat that does not fall along a transect line would get searched. It was also requested that the mussel searches include walking the banks on both sides of each tailwater to identify fresh mussel shells/middens. It was agreed upon by the IAG participants that interested parties would meet during the summer, 2003 for a site visit to the four tailwaters to assist in establishing the permanent fish and mussel sampling stations (transects) proposed for each tailwater. A revised draft study plan was distributed to the IAG in May, 2002. Minor comments received on the revised draft have been incorporated into this final study plan. Methods The tailwater fish and aquatic biota assessment will be conducted by Normandeau Associates Inc. (NAI) with assistance from Pennington and Associates, Inc. and will entail the following: Tailwater Fish Sampling Normandeau Associates will conduct intensive electrofishing, trap netting, seine netting and gill netting in the four tailwaters of the Yadkin Project during spring, summer and fall seasons. Spring sampling will be conducted in late April/May 2004 to document resident fish use of tailwater areas and to search for RTE redhorse species. Summer sampling will occur in August 2003 and fall sampling will be performed in November 2003. Permanent fish sampling stations (and electrofishing transects) will be established in each of the four Project tailwaters in June 2003 by agencies and interested participants from the Fish and Aquatics IAG. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft The primary objective during the seasonal fish sampling in each of the four tailwaters will be to capture as many fish species as possible. This will be accomplished by intensive boat electrofishing, gill netting, seine netting and trap netting over a 3 to 4 day period in each tailwater per season. Many fish are habitat specialists, therefore efforts will be made to sample all habitats present in the tailwaters, including deeper runs, pools, undercut banks and shallow shoals. Other objectives will include sampling during generation on and generation off (or reduced generation - this will depend on the season and flows) in each tailwater and sampling during low DO compared to normal DO time periods each season to see if these project operations effects fish movement. DO levels are more apt to be low and fluctuate more each day during the summer sampling period, therefore sampling in the spring period may only focus on peaking generation (no generation/generation) if daily DO fluctuations are small. During all tailwater fish sampling, temperature and DO profiles will be collected at selected stations in each tailwater under each operating scenario, such as during generation on, generation off (or reduced generation if higher flows) and low DO or not low DO time periods. Normandeau also plans on fishing each tailwater during daytime and nighttime periods over the four days of sampling planned for each tailwater during each season. Fishing at night can be very effective and at times can produce the largest catches and the most species. In addition to the water quality data collection during fish sampling, Normandeau has installed YSI continuous DO/temperature water quality monitors in all four tailwaters in late April2003 that will remain in place through November 2003. This data will also be analyzed to confirm changes in DO and temperature that will occur during each seasonal fish sampling period and to review seasonal conditions prior to sampling so that collections can be designed around daily changes in DO, temperature and flows (flow data will be provided by Yadkin). To evaluate the longitudinal and lateral extent of DO conditions in the tailwaters, in August and September 2003, beginning at each YSI continuous tailwater monitor, temperature and DO profiles will be taken at11/4 points along transects that will be spaced 11/4 mile apart (going downstream from the monitor). Transects will be added until temperature and DO conditions at consecutive transects are mixed (this effort is included within the Yadkin Water Quality study plan). Fish sampling methods for each tailwater (and each season) will be similar. The shock boat will be used to sample the shoreline (where water depth permits) and channel sections of the tailwaters traveling in a downstream direction. The shock boat uses Smith-Root electronics and will be set to pulsed DC current, >500v, 4 amps. Shocking runs will continue along chosen transects until two consecutive shocking runs fail to capture any new species for a given habitat (i.e. shoreline or channel habitat). The time for each boat shocking run will be recorded on the data sheets along with the number and species of fish collected. Total length (mm) and weight (gm) of fish captured will also be recorded on the field data sheets - a sub- sample of 50 randomly chosen individuals will be measured and weighted for abundant fish species captured. Gill nets will also be fished at the same time the boat shocking is being conducted. Experimental gill nets measuring 30.5 in long and 2.4 in deep, and constructed with four 7.6 in panels with mesh sizes of 2.54 cm, 5.08 cm, 7.62 cm and 10.16 cm of stretch monofilament will be used - these are the same sized gill nets recently used in 2000 by Progress Energy to sample for fish in the Yadkin Project reservoirs. These gill nets will be set prior to electrofishing in various locations/habitat types in the tailwater and their location (GPS), depth and habitat type will be recorded for each station. It has been our experience that the boat shocker can be used to effectively "drive" fish into the gill nets, especially in deeper tailraces where the boat shocker may not be entirely effective. Our intent is to keep the gill nets mobile, moving them to different locations or habitat types frequently, in an effort to capture as many species as possible during each operational scenario (gen. on/ gen. off, etc). Some gill nets will be moved around to sample in concert with the shock boat, and others will be used to fish deeper areas that the shock boat cannot 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft effectively fish. Gill nets will be fished at least every 4 to 6 hours and will not be left unattended or fished overnight. Trap nets may also be used, but their use and number of traps deployed will depend on their success at capturing fish species not collected via the shock boat and gill net. If it is determined that this gear type does not help capture additional fish species, at least one net will be set-up in each tailwater as a holding pen to keep alive redhorse species or other uncommon species that may need to have their identity verified by other experts. Seine nets will also be used in all four tailwaters to try to collect smaller fish species that may be present. Tailwater Macroinvertebrate Sampling Normandeau and Pennington and Associates, Inc. will search for mussels and collect macroinvertebrates at permanent stations and transects set-up in the Project tailwaters during summer (August, 2003), fall (November, 2003), and spring (May, 2004). These permanent macroinvertebrate stations and mussel transects in each of the four tailwaters will be located in early June, 2003 by agency personnel and interested participants from the Fish and Aquatics IAG. Once a station or transect location is picked by the IAG, its position will be pinpointed with GPS and all future macroinvertebrate sampling or mussel searches will be taken from the same locations. Normandeau and Pennington propose to set-up 2 transects in each of the developments tailwaters - one transect near each powerhouse and the other located downstream in the lower tailwater (to be determined in field by Normandeau and the IAG members). Three 2 m2 macroinvertebrate samples will be collected from each transect at 25%, 50% and 75% of the distance along each transect (six samples from each tailwater per each sample period). However, these station locations will ultimately depend on the consensus from participants that attend the early June 2003 field trip to locate the stations. Mussel searches will also be conducted along these same transect lines. In deep water (>4 ft), an underwater airlift will be used to collect macroinvertebrate samples (2 m2 sample size) at each station along the transect line (in shoal water, a kick net will be used to collect the samples). Macroinvertebrates collected will be preserved on-site and returned to PAI's lab for sorting and identification of species. Mussel searches will also be conducted each season by divers swimming along the length of each transect line (length dependent on the wetted width of each tailwater at time of sampling). Divers will search at least one meter upstream and downstream of each transect line (2 in wide band along the entire transect), but this will greatly depend on visibility at the time of the search. Additionally, mussel searches will done by walking along the shoreline of each tailwater looking for mussel shells and by having divers search in areas identified by participants as good mussel habitat that is not located along a transect line. Any live RTE mussel species located during these searches will be identified, returned to where it was found (if it was removed from the water), and its location recorded with GPS. The location of any relic mussel shells found will also be recorded and the shells collected and identified. During the fisheries and macroinvertebrate sampling and mussel searches planned for August 2003, the divers will also describe the habitat types found in each tailwater area. Depending on visibility, divers will take representative U/W pictures of the habitat found along each transect line and also pictures of the chosen macroinvertebrate sampling stations. Carolina and Robust Redhorse Searches During the seasonal tailwater fish sampling, Normandeau will attempt to capture the Carolina and Robust redhorse species that are known to occupy habitat in the Pee Dee River downstream of the Falls 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft development and the Progress Energy dams. Normandeau will specifically target these fish in the Yadkin tailwaters during their spring spawning period in late April-early May, 2004, which is when the spring tailwater sampling begins. Electrofishing and gill nets will be used to try to capture these fish (see methods above in Tailwater Fish Sampling). Most of the available spawning habitat for the robust redhorse exists in the Project tailwaters. The shoal areas at each development will be targeted for spawning redhorse species when water temperatures are between 18° C and 24° C. The spring tailwater fish survey will begin at the Falls tailwater, and gradually (over a 2 week period) move up the river ending the spring fish sampling at the High Rock tailwater. Any robust or Carolina redhorse captured will be identified, measured, weighted and released unharmed. If identification is difficult, the fish will be safely held in a trap net until it can be properly identified by experts (Progress Energy personnel are willing to help with identification of these fish). Data Collection and Reporting Schedule Normandeau proposes to conduct the tailwater fish, macroinvertebrate and mussel field evaluations in each of the four Project tailwaters during spring (April/May 2004), summer (August 2003) and late fall (November 2003) sample periods. Searches for the Carolina and robust redhorse's will be conducted seasonally during the tailwater fish assessments. Locations of the proposed permanent fish, mussel and macroinvertebrate stations and transects will be selected in June 2003 by interested members of the Fish and Aquatics IAG. Results of the fish, macroinvertebrate and mussel evaluations will be reported in draft and final study reports. A draft study report for the Tailwater fish sampling will be prepared and distributed to the Fish and Aquatics IAG for review and comment by August 31, 2004, approximately three months after the completion of data collection. IAG comments will be addressed in a final study report that will be completed by November 30, 2004. A draft study report for the Tailwater macroinvertebrate and mussel evaluations will be prepared and distributed to the Fish and Aquatics IAG by the 3rd quarter of 2004 and after IAG comments are addressed, the final report will be distributed by the 4th quarter of 2004. Interim results, such as results of seasonal tailwater fish sampling, and mussel searches, may be shared with the IAG as such information becomes available, prior to completion of the draft study report. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, Inc. Tailwater Fish & Aquatic Biota Assessment Draft APPENDIX 2 Length Frequency Distributions for Selected Species from High Rock, Tuckertown, Narrows, and Falls Tailwaters. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=55 100 N 80 v= 0 60- 40- E z 20 0 10 3 3 0 4 0 1 4 3 10 8 5 2 2 0 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Summer 2003 High Rock N=63 100 N 80 v= 0 60 d E 40 z 20 13 10 11 11 0 1 4 2 2 2 1 1 4 1 0 0 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Fall 2003 High Rock N=89 100 N 80 v= 0 60 d E 40 z 20 10 10 11 12 5 8 7 5 7 6 0 3 2 0 1 1 1 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Sorina 2004 Figure 1-1. Length frequency distribution of largemouth bass captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=3 100 80 I T 0 60- 40- z 20- 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 1 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112-Summer 2003 High Rock N=6 100 80 I T 0 60 E 40 Z 20- 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 0 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Fall 2003 High Rock N=2 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Sori na 2004 Figure 1-2. Length frequency distribution of striped bass captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=92 100 80 I T 0 60- 40- z 20 9 13 8 7 9 11 9 6 6 4 0 1 0 2 1 0 0 0 0 0 0 0 3 3 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094-Summer 2003 High Rock N=73 100 80 I T 0 60 E 40 Z 20 17 10 8 7 0 4 2 4 5 2 2 2 2 4 1 0 0 2 0 0 0 0 0 1 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Fall 2003 High Rock N=63 100 N 80 I T 0 60 E 40 Z 20 0 0 0 1 0 1 1 1 5 5 g 5 7 6 3 8 2 6 2 0 2 1 2 1 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Sori na 2004 Figure 1-3. Length frequency distribution of channel catfish captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=133 100 80 I T 0 60 Q 41 38 40 E Z 20 14 12 11 p 2 2 3 5 3 2 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125-Summer 2003 High Rock N=68 100 80 I T 0 60 E 40 Z 20 g 7 10 11 15 1 2 4 5 2 3 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Fall 2003 High Rock N=75 100 N 80 I T 0 60 E 40 Z 20 12 19, 12 0 2 7 6 7 6 4 0 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Sorina 2004 Figure 1-4. Length frequency distribution of bluegill captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=51 100 80 I T 0 60- 40- z 20 16 0 0 1 0 0 0 2 3 5 5 5 2 3 1 2 1 0 0 0 1 1 1 0 1 1 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129-Summer 2003 High Rock N=34 100- 1 80 I T 0 60 E 40 Z 20 0 0 0 0 1 0 0 0 3 5 4 3 4 2 3 4 1 1 2 0 0 0 0 1 0 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129 - Fall 2003 High Rock N=62 100 80 I T 0 60 E 40 Z 20 11 11 8 0 0 1 5 5 1 1 1 0 0 0 0 0 1 0 0 0 1 0 0 3 6 5 2 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129 - Sorina 2004 Figure 1-5. Length frequency distribution of black crappie captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=5 100 80 I T 0 60- 40- z 20- o 0 1 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 0 0 0 0 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 Total Length (mm) 30128-Summer 2003 High Rock N=6 100- 1 80 I T 0 60 E 40 Z 20 1 1 0 0 0 0 0 0 0 0 1 0 0 2 0 1 0 0 0 0 0 0 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 Total Length (mm) 30128 - Fall 2003 High Rock N=27 100 80 I T 0 60 E 40 Z 20 9 9 0 1 0 0 0 0 0 0 0 3 5 0 0 0 0 0 0 0 0 0 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 Total Length (mm) 30128 - Sori na 2004 Figure 1-6. Length frequency distribution of white crappie captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N = 113 100 80 I T 0 60 `m 40 40 24 22 z 20- g 6 0 0 1 1 4 3 0 0 0 0 0 0 0 0 0 0 0 3 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111-Summer 2003 High Rock N=82 100 80 I T 0 60 E 40 32 25 Z 20 8 11 0 0 0 0 0 0 0 0 4 0 1 1 0 0 0 0 0 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Fall 2003 High Rock N=58 100 N 80 I T 0 60 40 E z 20 17 21 0 0 0 2 2 1 0 0 8 4 2 1 0 0 0 0 0 0 0 0 - 77 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Sorina 2004 Figure 1-7. Length frequency distribution of white perch captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=55 100 80 I T 0 60- 40- Z 20 11 6 6 11 3 2 4 3 4 3 0 0 1 1 0 0 0 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015-Summer 2003 High Rock N=44 100 80 I T 0 60 E 40 z 20- 16 13 0 0 2 2 1 0 - 6 0 1 2 0 1 0 0 0 0 0 0 0 0 0 1 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Fall 2003 High Rock N=103 100 N 80 I T 0 60 E 40 28 z 20 9 17 11 6 5 0 0 1 3 4 4 2 ?. 2 2 2 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Sori na 2004 Figure 1-8. Length frequency distribution of gizzard shad captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft High Rock N=8 100 80 I T 0 60- 40- z 20- 2 0 0 0 0 0 0 0 0 0 1 0 3 0 2 0 0 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018-Summer 2003 High Rock N=40 100 80 I T 0 60 E 40 Z 20 10 0 0 0 0 0 0 2 4 4 1 4 5 3 3 3 1 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Fall 2003 High Rock N=19 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 0 2 3 5 4 3 1 1 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Sorina 2004 Figure 1-9. Length frequency distribution of threadfin shad captured in High Rock tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=78 100 N 80 v= 6 60 Z5 E 40 E z 20 15 15 14 0 3 3 1 1 2 3 5 3 7 4 1 1 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Summer 2003 Tuckertown N=79 100 N 80 v= 0 60 Z5 E 40 E z 20 15 9 5 10 10 8 0 4 4 2 1 3 4 3 1 0 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Fall 2003 Tuckertown N=79 100 N 80 ILL 60 d 40 z 20 5 5 7 9 11 20 6 p 1 2 1 3 _ _ 2 0 0 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Sprina 2004 Figure 1-10. Length frequency distribution of largemouth bass captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=15 100 80 I T 0 60- 40- z 20- 4 4 2 0 0 0 - 0 0 0 0 0 0 0 0 0 0 2 1 0 1 0 1 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112-Summer 2003 Tuckertown N=43 100 80 I T 0 60 E 40 Z 20- 0 0 2 4 4 0 2 1 3 .? 0 2 0 3 1 0 1 3 1 3 2 1 1 2 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Fall 2003 Tuckertown N=7 100 N 80 I T 0 60 E 40 Z 20 0 1 0 0 0 0 0 0 0 1 0 0 1 0 0 2 0 1 1 0 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Sori na 2004 Figure 1-11. Length frequency distribution of striped bass captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=41 100 80 I T 0 60- 40- z 20 13 13 12 0 0 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 0 0 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 Total Length (mm) 30011-Summer 2003 Tuckertown N=10 100 80 I T 0 60 E 40 Z 20- 2 4 2 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 Total Length (mm) 30011 - Fall 2003 Tuckertown N=4 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 1 0 0 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 Total Length (mm) 30011 - Sorina 2004 Figure 1-12. Length frequency distribution of blueback herring captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=37 100 N 80 I T 0 60- 40- z 20 0 0 0 0 0 0 1 1 0 7 3 2 2 0 1 5 2 7 2 0 0 1 2 0 1 0 0 - 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129-Summer 2003 Tuckertown N=78 100 80 I T 0 60 E 40 z 20 6 7 6 2 4 6 2 4 4 12 7 5 3 4 0 0 1 1 1 1 1 0 0 0 0 0 1 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129 - Fall 2003 Tuckertown N=27 100 80 I T 0 60 E 40 Z 20- 0 1 2 0 0 0 0 0 4,!,l 0 1 1 3 4 1 0 1 3 0 0 1 0 1 0 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129 - Spring 2004 Figure 1-13. Length frequency distribution of black crappie captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=43 100 N 80 I T 0 60- 40- z 20 13 16 0 0 0 0 0 0 0 0 0 1 3 1 0 _3.o 2 1 0 0 0 1 2 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 Total Length (mm) 30128-Summer 2003 Tuckertown N=29 100 80 I T 0 60 E 40 Z 20 0 1 3 2 1 1 0 0 0 0 0 0 0 0 0 1 5 6 1 1 1 1 1 4 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 Total Length (mm) 30128 - Fall 2003 Tuckertown N=20 100 80 I T 0 60 E 40 Z 20- 0 0 1 3 0 0 0 0 0 0 0 1 1 1 0 0 0 1 0 0 0 3 6 3 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 Total Length (mm) 30128 - Spri ng 2004 Figure 1-14. Length frequency distribution of white crappie captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N = 267 100 80 69 i 61 0 60 48 ? 40 32 33 Z 20 11 3 3 0 4 3 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125-Summer 2003 Tuckertown N = 112 100 80 I T 0 60- 40- E 24 29 Z 20 11 7 20 12 r p - M, 4 3 2 0 0 0 0 0 0 0 0 0 0 0 71 - 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Fall 2003 Tuckertown N=88 100- 1 80- I T 0 60 E 40 25 z 20- 8 9 13 15 9 O n 3 4 2 0 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Spring 2004 Figure 1-15. Length frequency distribution of bluegill captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=19 100 80 I T 0 60- 40- z 20- 0 0 0 0 0 0 0 0 0 0 0 0 0 3 1 3 4 4 1 3 0 0 0 0 - 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398-Summer 2003 Tuckertown N=31 100 80 I T 0 60 E 40 Z 20 9 11 2 2 0 0 0 0 0 0 0 0 0 0 1 2 2 0 2 0 0 0 0 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398 - Fall 2003 Tuckertown N=6 100 N 80 I T 0 60 E 40 Z 20 0 0 2 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 1 1 0 0 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398 - Sori na 2004 Figure 1-16. Length frequency distribution of redear sunfish captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=100 100 80 I T 0 60- 40- z 20 13 16 14 12 2 2 1 0 0 0 3 5 2 4 8 5 5 5 0 2 0 1 0 0 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094-Summer 2003 Tuckertown N = 118 100 80 I T 0 60 E 40 Z 20 12 12 10 8 15 14 11 7 6 6 0 5 2 1 1 3 1 0 0 0 0 0 0 0 4 Ell 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Fall 2003 Tuckertown N=73 100 N 80 I T 0 60 E 40 z 20 16 0 2 0 0 0 0 0 5 6 7 4 6 2 4 3 5 7 2 2 0 1 1 p 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Sori na 2004 Figure 1-17. Length frequency distribution of channel catfish captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=8 100 80 I T 0 60- 40- z 20 1 0 0 0 0 1 1 0 0 2 0 0 0 1 2 0 0 0 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375-Summer 2003 Tuckertown N=1 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375 - Fall 2003 Tuckertown N=8 100 80 I T 0 60 E 40 Z 20- 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 1 0 0 0 0 1 1 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375 - Spri ng 2004 Figure 1-18. Length frequency distribution of blue catfish captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=147 100 80 I T 0 60 48 Q 40 35 E Z 20 10 15 11 4 1 0 6 5 5 0 0 1 4 0 0 1 1 0 0 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111-Summer 2003 Tuckertown N=108 100 80 I T 0 60 42 40 Z 20 20 20 9 ? 0 0 1 = 3 4 0 2 0 5 0 1 0 1 0 0 0 0 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Fall 2003 Tuckertown N=63 100 N 80 I T 0 60 E 40 19 20 Z 20 12 0 0 0 0 2 1 5 0 1 1.1 0 1 0 0 0 0 0 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Sori na 2004 Figure 1-19. Length frequency distribution of white perch captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N = 299 100 80 LL 0 60 56 58 43 Q 40 35 22 L Z 20 [ 1 15 9 17 12 10 5 0 1 0 0 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015-Summer 2003 Tuckertown N=62 100- 1 80- I T 0 60 E 40 Z 20 14 11 7 5 7 7 0 0 3 3 0 1 1 3 0 0 0 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Fall 2003 Tuckertown N=53 100 N 80 I T 0 60 E 40 z 20 6 9 13 10 7 , 0 0 0 0 0 0 0 3 3 2 I ?. 0 0 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Sori na 2004 Figure 1-20. Length frequency distribution of gizzard shad captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Tuckertown N=53 100 80 I T 0 60- 40- z 20- 15 0 1 0 2 1 2 3 6 6 4 8 2 2 0 0 0 0 0 1 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018-Summer 2003 Tuckertown N=50 100- 1 80- I T 0 60 E 40 z 20- 15 0 0 0 0 0 0 1 4 3 5 6 4 5 0 0 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Fall 2003 Tuckertown N=69 100 N 80 I T 0 60 E 40 22 26 z 20 14 0 1 0 0 0 0 0 0 0 0 0 1 5 0 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Sorina 2004 Figure 1-21 Length frequency distribution of threadfin shad captured in Tuckertown tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows 155 N = 245 100 N 80 v= 6 60 d E 40 z 20 17 9 9 14 7 9 4 4 0 2 4 5 4 1 1 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Summer 2003 Narrows N=96 100 N 80 ILL 6 60 42 40 E z 20 0 5 0 3 4 5 10 5 3 4 6 8 0 1 0 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Fall 2003 Narrows N=52 100 N 80 ILL 6 60 Z5 E 40 z 20 0 -- 6 1 0 1 4 3 9 2 7 6 -fl 5 F -1 4 F 3 0 1 0 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Sorina 2004 Figure 1-22. Length frequency distribution of largemouth bass captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=14 100 80 I T 0 60- 40- z 20 2 2 1 0 0 0 0 0 0 3 0 2 0 1 2 0 1 0 0 0 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112-Summer 2003 Narrows N=18 100 80 I T 0 60 E 40 Z 20- 0 2 2 3 2 0 0 0 1 3 0 2 2 0 0 0 0 0 0 1 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Fall 2003 Narrows N=7 100 80 I T 0 60 E 40 Z 20- 0 0 0 1 0 0 1 0 0 0 0 0 3 1 0 0 0 0 0 0 0 0 1 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Spri ng 2004 Figure 1-23. Length frequency distribution of striped bass captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=2 100 80 I T 0 60- 40- z 20- 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 Total Length (mm) 30011-Summer 2003 Narrows N=41 100 80 I T 0 60 E 40 Z 20 0 0 1 1 2 1 0 0 0 0 0 0 1 0 5 6 7 5 5 0 0 0 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 Total Length (mm) 30011 - Fall 2003 Narrows N=18 100 N 80 I T 0 60 E 40 Z 20 11 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 2_j 0 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 205 210 215 Total Length (mm) 30011 - Sorina 2004 Figure 1-24. Length frequency distribution of blueback herring captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=16 100 80 I T 0 60- 40- z 20- 1 0 0 0 3 0 0 1 0 0 4_3_1 0 0 0 0 0 1 1 2 0 0 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398-Summer 2003 Narrows N=5 100 80 I T 0 60 E 40 Z 20- 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 1 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398 - Fall 2003 Narrows N=14 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 2 0 0 2 2 1 1 1 0 1 3 1 0 0 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398 - Sorina 2004 Figure 1-25. Length frequency distribution of redear sunfish captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=241 100 80 IT 64 `0 60 51 Q 40- 34 E 25 Z 20 7 10 10 1 1 3 0 1 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125-Summer 2003 Narrows N=49 100 80 I T 0 60 E 40 Z 20 18 0 0 0 1 3 8 4 1 0 0 0 0 0 0 0 0 0 0- 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Fall 2003 Narrows N=100 100 N 80 I T 0 60 E 40 33 23 Z 20 16 9 0 0 3 2 3 4 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Sorina 2004 Figure 1-26. Length frequency distribution of bluegill captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=15 100 80 I T 0 60- 40- z 20- 1 0 0 0 0 0 0 0 0 0 0 1 0 2 1 0 1 1 2 2 2 0 0 1 1 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129-Summer 2003 Narrows N=16 100- 1 80 I T 0 60 E 40 Z 20 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 1 3 1 2 2 3 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129 - Fall 2003 Narrows N=1 100 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 Total Length (mm) 30129 - Sorina 2004 Figure 1-27. Length frequency distribution of black crappie captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=92 100 80 I T 0 60- 40- z 20 9 6 2 2 10 4 4 5 10 8 7 6 4 6 3 2 3 0 1 0 0 0 0 0 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094-Summer 2003 Narrows N=38 100 80 I T 0 60 E 40 Z 20- 0 0 0 0 0 0 0 2 4 2 1 2 3 1 6 2 5 4 1 1 1 0 1 2 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Fall 2003 Narrows N=72 100 N 80 I T 0 60 E 40 z 20 15 3 1 1 0 2 0 0 1 2 2 6 4 6 5 5 5 _2'[8;j 0 2 0 0 - 9-1 - F-1 FIE 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Sori na 2004 Figure 1-28. Length frequency distribution of channel catfish captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=90 100 80 I T 0 60- 40- Z 20 19 20 11 7 6 5 7 0 - 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375-Summer 2003 Narrows N=7 100 80 I T 0 60 E 40 Z 20- 0 0 0 0 0 0 0 0 0 3 1 1 0 0 0 1 0 0 0 0 1 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375 - Fall 2003 Narrows N=24 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 1 0 1 4 4 3 4 4 1 2 0 0 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375 - Sori na 2004 Figure 1-29. Length frequency distribution of blue catfish captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows 115 N = 318 100 80 LL 0 60 s 40 E 32 22 22 27 Z 20 19 20 9 11 13 9 6 6 4 0 2 1 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015-Summer 2003 Narrows N=14 100- 1 80- I 0 60 E 40 Z 20 0 4 5 1 0 0 0 3 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Fall 2003 Narrows N=77 100 N 80 LL 0 60 E 40 Z 20 19 17 0 1 9 5 3 4 3 1 0 0 0 0 0 0 0 1 0 0 0 - 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Sori na 2004 Figure 1-30. Length frequency distribution of gizzard shad captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N=17 100 80 I T 0 60- 40- z 20 0 0 0 0 0 0 2 1 0 2 2 2 6 2 0 0 0 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018-Summer 2003 Narrows N=51 100 80 I T 0 60 E 40 23 Z 20 11 0 0 0 0 0 0 0 0 0 2 4 5_2'2 1 0 0 0 0 1 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Fall 2003 Narrows N=6 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 0 0 0 1 1 2 1 1 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Sorina 2004 Figure 1-31. Length frequency distribution of threadfin shad captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Narrows N = 327 100 80 I T ° 60 44 46 51 45 E 40 32 28 28 Z 20 7177 13 19 0 0 0 _WdMMMWWjjMM 0 1 0 0 1 5 4 7 1 2 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111-Summer 2003 Narrows N=78 100 80 I T 0 60 E 40 23 Z 20 g 15 10 0 0 1 0 0 0 0 0 0 0 5 4 4 4 3 0 0 1 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Fall 2003 Narrows N=23 100 N 80 I T 0 60 E 40 Z 20 0 0 0 1 0 1 0 0 0 3 3 4 6. 0 1 1 1 0 1 0 1 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Sori na 2004 Figure 1-32. Length frequency distribution of white perch captured in Narrows tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N= 59 100 N 80 ILL 6 60 d E 40 z 20 19 12 2 1 0 0 0 2 2 6 3 3 5 2 2 0 0 p 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Summer 2003 Falls N= 89 100 N 80- . = 6 60 Z5 n 40 3 22 z 20 9 6 9 10 15 6 1 4 1 1 1 0 F° 1 2 p 1 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Fall 2003 Falls N = 102 100 N 80 ILL 6 60 Z5 E 40 E 24 z 20 10 8 12 9 15 8 0 4 2 p 1 1 3 e. 3 p 2 0 20 55 90 125 160 195 230 265 300 335 370 405 440 475 510 545 580 Total Length (mm) 30127 - Sorina 2004 Figure 1-33. Length frequency distribution of largemouth bass captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=2 100 80 I T 0 60- 40- z 20- 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112-Summer 2003 Falls N=15 100 80 I T 0 60 E 40 Z 20- 1 2 0 3 2 0 0 0 0 0 1 0 0 0 1 0 3 1 0 1 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Fall 2003 Falls N=1 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 90 120 150 180 210 240 270 300 330 360 390 420 450 480 510 540 570 600 630 660 690 720 750 780 Total Length (mm) 30112 - Sori na 2004 Figure 1-34. Length frequency distribution of striped bass captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=67 100 80 I T 0 60- 40- z 20 5 5 6 4 8 4 3 5 7 6 8 2 0 0 0 0 1 1 0 1 0 1 , 0 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398-Summer 2003 Falls N=54 100 80 I T 0 60 E 40 Z 20 12 0 0 0 0 0 0 2 1 0 0 1 0 _ 2 2 2 0 3 6 1 6 7 5 5 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398 - Fall 2003 Falls N=55 100 80 I T 0 60 E 40 Z 20 g 10 10 8 6 0 0 0 0 0 0 0 0 0 3 0 0 3 3 2 1 1 0 0 0 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 Total Length (mm) 30398 - Spring 2004 Figure 1-35. Length frequency distribution of redear sunfish captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=137 100 80 I T 0 60 49 40 34 26 z 20 11 g 0 1 0 3 3 1 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125-Summer 2003 Falls N=87 100- 1 80 I T 0 60 40 29 z 20 12 18 11 0 4 2 1 3 I I 6 1 0 0 0 0 0 0 0 0 0 0 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Fall 2003 Falls N=81 100 80 I T 0 60 ? 40 19 23 17 z 20 0 1 0 3 7 4 2 3 1 0 0 0 0 0 0 0 0 1 0 30 45 60 75 90 105 120 135 150 165 180 195 210 225 240 255 270 285 300 315 330 Total Length (mm) 30125 - Sorina 2004 Figure 1-36. Length frequency distribution of bluegill captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=13 100 80 I T 0 60- 40- z 20- 0 0 0 0 0 1 1 0 0 2 1 0 1 0 1 2 1 0 1 0 0 1 1 0 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094-Summer 2003 Falls N=12 100 80 I T 0 60 E 40 Z 20- 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 2 1 1 1 2 1 0 2 1 0 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Fall 2003 Falls N=19 100 N 80 I T 0 60 E 40 Z 20 0 1 0 0 0 0 0 0 0 0 0 2 0 2 0 2 2 4 1 3 0 0 2 0 0 50 75 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 500 525 550 575 600 625 Total Length (mm) 30094 - Sori na 2004 Figure 1-37. Length frequency distribution of channel catfish captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=40 100 80 I T 0 60- 40- z 20 14 10 0 0 0 0 0 0 1 1 0 2 3 4 1 0 1 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375-Summer 2003 Falls N=19 100 80 I T 0 60 E 40 Z 20- 0 0 0 0 0 0 1 0 0 1 3 3 1 6 3 1 0 0 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375 - Fall 2003 Falls N=10 100 N 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 0 0 0 2 0 1 2 3 2 0 0 0 0 0 0 0 0 40 80 120 160 200 240 280 320 360 400 440 480 520 560 600 640 680 720 760 800 840 880 920 Total Length (mm) 30375 - Sori na 2004 Figure 1-38. Length frequency distribution of blue catfish captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=86 100 80 I T 0 60 ? 40 Z 20 12 15 14 10 7 8 0 0 4 4 1 0 0 0 0 0 2 0 0 4 1 2 2 F1 rM 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015-Summer 2003 Falls N=120 100 80 I T 0 60 Q 40 38 E Z 20 20 9 13 17 0 0 6 1 0 1 2 0 0 3 3 5 1 IM - - El 0 0 0 0 1 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Fall 2003 Falls N=92 100 N 80 I T 0 60- 40- E 27 29 z 20 16 8 0 1 3 1 1 0 0 0 0 2 0 0 0 0 1 0 0 1 2 0 50 70 90 110 130 150 170 190 210 230 250 270 290 310 330 350 370 390 410 430 450 470 490 Total Length (mm) 30015 - Sori na 2004 Figure 1-39. Length frequency distribution of gizzard shad captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=87 100 80 I T 0 60 ? 40 Z 20 14 16 14 11 7 8 0 0 0 0 1 3 4 5 0 1 F9 7? 1 1 0 0 0 0 0 0 0 1 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018-Summer 2003 Falls N=23 100 80 I T 0 60 E 40 Z 20 0 0 0 0 0 0 0 1 1 0 1 6 3 4 5 1 1 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Fall 2003 Falls N=74 100 N 80 I T 0 60 E 40 z 20 14 23 16 15 0 0 0 0 0 0 0 0 0 2 3 1 0 0 0 0 0 0 0 0 0 0 0 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 Total Length (mm) 30018 - Sorina 2004 Figure 1-40. Length frequency distribution of threadfin shad captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft Falls N=107 100 80 I T 0 60 40 24 z 20 16 18 g 11 2 1 0 0 0 0 0 3 4 4 2 5 7 0 1 0 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111-Summer 2003 Falls N=68 100 80 I T 0 60 E 40 23 z 20 g 13 10 0 0 0 0 0 0 0 0 0 5 5 2 1 0 0 0 0 0 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Fall 2003 Falls N=76 100 N 80 I T 0 60 40 E 21 z 20 10 13 g 0 0 0 1 1 3 6 3 2 0 1 0 0 0 0 0 0 6 0 75 85 95 105 115 125 135 145 155 165 175 185 195 205 215 225 235 245 255 265 275 285 Total Length (mm) 30111 - Sorina 2004 Figure 1-41. Length frequency distribution of white perch captured in Falls tailwater by season. All gear types combined. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft APPENDIX 3 Narrows Tailwater Long Term Monitor and Plant Generation Data for 2 24- Hour Time Periods Investigating Fish Abundances and Diversity During Periods of Normal and Low Dissolved Oxygen Levels 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft August 31- September 1, 2003 DATE hour MeanTemp MeanDO Total Generation (MWh) Total Outflow (cfs) Log (Mean DO) Log (Total Outflow) 8/30/2003 23 26.495 5.285 44 3415 0.723044992 3.533390708 8/31/2003 0 26.34 5.94 4 350 0.773786445 2.544068044 8/31/2003 1 26.3175 6.0475 4 350 0.781575877 2.544068044 8/31/2003 2 26.3225 6.01 4 350 0.778874472 2.544068044 8/31/2003 3 26.34 6.0675 4 350 0.783009785 2.544068044 8/31/2003 4 26.34 6.105 4 350 0.785685668 2.544068044 8/31/2003 5 26.3275 6.065 4 350 0.782830805 2.544068044 8/31/2003 6 26.3 6.015 4 350 0.779235632 2.544068044 8/31/2003 7 26.545 4.14 8 855 0.617000341 2.931966115 8/31/2003 8 26.6975 3.07 44 3790 0.487138375 3.57863921 8/31/2003 9 26.7275 2.975 44 3790 0.47348697 3.57863921 8/31/2003 10 26.775 3.005 44 3790 0.477844476 3.57863921 8/31/2003 11 26.8125 3.19 44 3790 0.503790683 3.57863921 8/31/2003 12 26.795 2.2875 40 3620 0.359361103 3.558708571 8/31/2003 13 26.66 1.6375 0 0 0.214181309 0 8/31/2003 14 26.5575 1.705 0 0 0.231724383 0 8/31/2003 15 26.43 1.63 0 0 0.212187604 0 8/31/2003 16 26.3175 2.0625 0 0 0.314393957 0 8/31/2003 17 26.3675 5.2325 1 350 0.718709237 2.544068044 8/31/2003 18 26.505 6.2375 2 350 0.795010559 2.544068044 8/31/2003 19 26.525 6.4 4 350 0.806179974 2.544068044 8/31/2003 20 26.485 6.48 5 620 0.811575006 2.792391689 8/31/2003 21 26.435 6.47 4 350 0.810904281 2.544068044 8/31/2003 22 26.375 6.565 4 350 0.81723473 2.544068044 8/31/2003 23 26.37 6.4 4 350 0.806179974 2.544068044 9/1/2003 0 26.41 6.24 4 350 0.79518459 2.544068044 9/1/2003 1 26.4325 6.075 3 350 0.783546282 2.544068044 9/1/2003 2 26.4225 6.1675 2 350 0.790109158 2.544068044 9/1/2003 3 26.4525 6.245 2 350 0.795532443 2.544068044 9/1/2003 4 26.4775 6.265 2 350 0.796921075 2.544068044 9/1/2003 5 26.49 6.2525 2 350 0.7960537 2.544068044 9/1/2003 6 26.495 6.2875 2 350 0.798477998 2.544068044 9/1/2003 7 26.5025 6.0325 2 350 0.780497331 2.544068044 9/1/2003 8 26.4825 6.3025 2 350 0.799512854 2.544068044 9/1/2003 9 26.4825 6.28 2 350 0.797959644 2.544068044 9/1/2003 10 26.46 6.1525 3 350 0.789051622 2.544068044 9/1/2003 11 26.4425 6.315 2 350 0.800373355 2.544068044 9/1/2003 12 26.415 6.2525 2 350 0.7960537 2.544068044 9/1/2003 13 26.585 6.3175 2 350 0.800545251 2.544068044 9/1/2003 14 26.69 4.4375 37 2955 0.647138366 3.470557485 9/1/2003 15 26.6725 3.73 88 4800 0.571708832 3.681241237 9/1/2003 16 26.69 3.7925 89 4820 0.578925589 3.683047038 9/1/2003 17 26.6625 3.6325 88 4800 0.560205623 3.681241237 9/1/2003 18 26.655 3.5325 89 4840 0.548082171 3.684845362 9/1/2003 19 26.6025 3.355 88 4800 0.525692525 3.681241237 9/1/2003 20 26.54 3.2025 89 4840 0.505489138 3.684845362 9/1/2003 21 26.545 3.1675 88 4800 0.500716624 3.681241237 9/1/2003 22 26.5525 2.975 83 6855 0.47348697 3.836007459 9/1/2003 23 26.5625 3.315 82 6715 0.520483533 3.827046017 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft November 7 to 8, 2003 DATE MeanTemp MeanDO MeanPctSat hour Total Generation ((MWh) Total Outflow ((cfs) Log DO Log CFS 11/7/2003 17.96 7.4175 0.788003173 0 1 350 0.870258 2.544068 11/7/2003 17.8525 8.48 0.898997028 1 3 350 0.928396 2.544068 11/7/2003 17.8175 8.61 0.912116231 2 3 350 0.935003 2.544068 11/7/2003 17.835 8.665 0.918277104 3 3 350 0.937769 2.544068 11/7/2003 17.925 8.635 0.916801514 4 3 350 0.936262 2.544068 11/7/2003 18.0075 8.5 0.904006317 5 3 350 0.929419 2.544068 11/7/2003 18.0275 8.57 0.911826432 6 3 350 0.932981 2.544068 11/7/2003 18.0775 7.5725 0.806466872 7 3 700 0.879239 2.845098 11/7/2003 18.1075 6.4775 0.690326279 8 67 5110 0.811407 3.708421 11/7/2003 18.1175 6.5925 0.702726163 9 70 5310 0.81905 3.725095 11/7/2003 18.1225 6.5175 0.694802914 10 71 5375 0.814081 3.730378 11/7/2003 18.1775 6.6025 0.704662536 11 70 5310 0.819708 3.725095 11/7/2003 18.245 6.5925 0.704571787 12 70 5310 0.81905 3.725095 11/7/2003 18.3 6.6 0.706168754 13 70 5310 0.819544 3.725095 11/7/2003 18.36 6.6125 0.708373893 14 70 5310 0.820366 3.725095 11/7/2003 18.3925 6.71 0.719299258 15 70 5310 0.826723 3.725095 11/7/2003 18.375 6.6175 0.709130512 16 68 5170 0.820694 3.713491 11/7/2003 18.365 6.6975 0.717558286 17 68 5170 0.825913 3.713491 11/7/2003 18.3575 6.54 0.700573947 18 69 5250 0.815578 3.720159 11/7/2003 18.36 6.69 0.716678023 19 68 5170 0.825426 3.713491 11/7/2003 18.36 6.745 0.722571534 20 68 5170 0.828982 3.713491 11/7/2003 18.3525 6.76 0.724066242 21 68 5170 0.829947 3.713491 11/7/2003 18.375 6.845 0.733509268 22 68 5170 0.835373 3.713491 11/7/2003 18.325 7.595 0.81302076 23 65 4985 0.880528 3.697665 11/8/2003 18.2225 8.5475 0.913081238 0 2 350 0.931839 2.544068 11/8/2003 18.155 8.6425 0.921952011 1 3 350 0.936639 2.544068 11/8/2003 18.11 8.7025 0.927497321 2 3 350 0.939644 2.544068 11/8/2003 18.0875 8.8 0.93745553 3 3 350 0.944483 2.544068 11/8/2003 18.0875 8.835 0.941183524 4 4 350 0.946207 2.544068 11/8/2003 18.155 8.8325 0.942230086 5 3 350 0.946084 2.544068 11/8/2003 18.2175 8.945 0.955454653 6 3 350 0.95158 2.544068 11/8/2003 18.2425 8.29 0.885953435 7 2 350 0.918555 2.544068 11/8/2003 18.08 7.2825 0.775744608 8 0 0 0.86228 0 11/8/2003 17.9675 5.7 0.605727921 9 0 0 0.755875 0 11/8/2003 17.905 5.355 0.568320189 10 0 0 0.728759 0 11/8/2003 17.8975 4.1575 0.441160952 11 0 0 0.618832 0 11/8/2003 17.9625 3.4125 0.362579441 12 0 0 0.533073 0 11/8/2003 18.0675 3.2325 0.344205146 13 0 0 0.509539 0 11/8/2003 18.1575 3.27 0.348858889 14 0 0 0.514548 0 11/8/2003 18.2125 2.8675 0.306255861 15 0 0 0.457503 0 11/8/2003 18.1175 6.055 0.645269982 16 0 0 0.782114 0 11/8/2003 18.1125 8.1775 0.871588809 17 3 350 0.912621 2.544068 11/8/2003 18.0775 6.735 0.717339182 18 0 0 0.828338 0 11/8/2003 17.8925 5.97 0.633443383 19 0 0 0.775974 0 11/8/2003 17.8175 5.0525 0.535259303 20 0 0 0.703506 0 11/8/2003 17.785 3.97 0.420292286 21 0 0 0.598791 0 11/8/2003 17.7425 3.98 0.420979342 22 0 0 0.599883 0 11/8/2003 17.6775 3.5725 0.377365609 23 0 0 0.552972 0 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. Tailwater Fish & Aquatic Biota Assessment Draft APPENDIX 4 Benthic Macroinvertebrate Survey, Yadkin River, September 2003-June 2004 for Normandeau Associates, Inc. by Pennington and Associates, Inc. 19556.001 Tailwater Fisheries Assessment.doc 3/11/05 Normandeau Associates, inc. BENTHIC MACROINVERTEBRATE SURVEY YADKIN RIVER YADKIN PROJECT (FERC No. 2197) SEPTEMBER 2003 - JUNE 2004 FOR NORMANDEAU ASSOCIATES, INC 25 NASHUA ROAD BEDFORD, NH 03110-5500 By Pennington and Associates, Inc 570 East loth Street Cookeville, Tennessee 38501 931-526-6038 TABLE OF CONTENTS Page EXECUTIVE SUMMARY ................................................................. .................................... 1 INTRODUCTION ................................................................................. .................................... 2 MATERIALS AND METHODS ....................................................... .................................... 8 LABORATORY METHODS ................................................................. .................................... 9 SUBSTRATE DETERMINATION ........................................................ .................................. 10 COMMUNITY STRUCTURE MEASURES ......................................... .................................. 10 BIOTIC INDEX ...................................................................................... .................................. 11 PHYSICAL AND CHEMICAL PARAMETERS .................................. .................................. 14 RESULTS AND DISCUSSON .......................................................... .................................. 15 FRESHWATER MUSSELS ................................................................... .................................. 17 BENTHIC MACROINVERTEBRATES ............................................... .................................. 18 REFERENCES ....................................................................................... .................................. 40 APPENDIX ............................................................................................. .................................. 42 EXECUTIVE SUMMARY A study of the benthic macroinvertebrate and freshwater mussel populations was conducted in the tailwaters of the Falls Dam, Narrows (Badin) Dam, Tuckertown Dam and High Rock Dam on the Yadkin River. The study was conducted during the Summer (September, 2003), Fall (November 2003) and Spring (June 2004) seasons. Two transects were established in each tailwater. Along each transect -2m2 benthic macroinvertebrate samples were taken by SCUBA at 25%, 50%, and 75% of the distance along each transect for total of 6 at each tailwater and 24 for each season. A total of 72 discrete benthic samples were taken from the four tailwaters throughout the study by SCUBA divers using an airlift collection device. A total of 7 freshwater unionid mussels were taken during the study with most found (575 live individuals) in the Falls Dam Tailwater. Elliptio complanta was the dominant species with Lampsilis radiata and Elliptio cf. lanceolata also abundant. All other mussels were represented by a few live individuals. There were 6 unionid mussel species and 22 live individuals taken in the Narrows Dam Tailwater while only one species (Utterbackia imbecillis) was found in the Tuckertown and High Rock tailwaters. No federally listed species were found. Anodonta implicata and Lampsilis radiata are considered state threatened while Villosa delumbis is considered significantly rare by the North Carolina Heritage Program A minimum of 99 species of benthic macroinvertebrates was taken from all four tailwaters and all three seasons. The highest number of species (53) was taken from Falls Dam in June, followed by 48 from the Narrows Dam Tailwater in September, 46 from High Rock in June, 44 from Tuckertown in June, 43 from High Rock in November and 42 from Falls Dam in September. High Rock Dam Tailwater in September had the lowest number of species with 29. Biotic indices indicate that the benthic fauna in the four tailwaters are residing under "Poor" to "Fair" water quality conditions. Pennington and Associates, Inc Page- 1 - Appendix 4.doc 3/11/2005 INTRODUCTION Alcoa Power Generating Inc. (APGI) is the licensee for the Yadkin Hydroelectric Project. The Yadkin Project is currently licensed by the Federal Energy Regulatory Commission (FERC) as Project No. 2197. This license expires in 2008 and APGI must file a new license application with FERC on or before April 30, 2006, to continue operation of the Project. The Yadkin Project consists of four reservoirs (figures 1-4), dams and powerhouses (High Rock, Tuckertown, Narrows, and Falls) located on a 38-mile stretch of the Yadkin River in central North Carolina. The project generates electricity to support the power needs of Alcoa's Badin Works, to support its other aluminum operations, or is sold on the open market. Falls, Narrows (Badin) and Tuckertown are located in Montgomery and Stanley Counties, North Carolina. High Rock is located in Davidson and Rowan Counties, North Carolina. As part of the relicensing progress, APGI prepared and distributed, in September 2002, an Initial Consultation Document (ICD), which provides a general overview of the Project. Agencies, municipalities, non-governmental organizations and members of the public were given an opportunity to review the ICD and identify information and studies that were needed to address relicensing issues. To further assist in the identification of issues of resource and data/study needs throughout the relicensing process. APGI formed several Issue Advisory Groups (IAGs). IAGs also had the opportunity to review and comment on Draft Study Plans. A Draft Study Plan was developed in response to comments on the ICD and through discussions with the Fish and Aquatics IAG to provide additional necessary information for consideration in the relicensing process. The following issues were raised during the initial consultation regarding tailwater fish and aquatic biota at the Yadkin Project: • Effects of Yadkin Project reservoir releases on tailwater fish, macroinvertebrates and aquatic habitat • Current status of rare, threatened and endangered (RTE) aquatic species at the Yadkin Project that could be impacted by Project operations Pennington and Associates, Inc Page- 2 - Appendix 4.doc 3/11/2005 To address a portion of these issues, Pennington and Associates, Inc. was contracted by Normandeau Associates, Inc. to conduct a benthic macroinvertebrate and freshwater mussel survey in the tailwaters of the four dams. The surveys were to: • Inventory and assess the benthic macroinvertebrate and mussel populations in the Project tailwaters on a seasonal basis to develop baseline data that can be used to detect changes in these communities over time. Evaluate impacts of existing Project operations on the tailwater macroinvertebrate community and describe tailwater habitats. • Search for RTE mussel species in Project tailwaters. Collections for benthic macroinvertebrates and freshwater mussels in Falls Dam Tailwater were made on September 13 and November 8, 2003 and on June 5, 2004. The surveys in Narrows Dam Tailwater were accomplished on September 14 and November 9, 2003 and June 6, 2004. The dates for the Tuckertown Tailwater collections were September 20 and November 15, 2003 and June 12, 2004. The High Rock Tailwater survey was conducted on September 21 and November 16, 2003 and on June 13, 2004. Pennington and Associates, Inc Page- 3 - Appendix 4.doc 3/11/2005 Pennington and Associates, Inc Page- 4 - Appendix 4.doc 3/11/2005 Figure 1. Falls Dam, Yadkin River. Pennington and Associates, Inc Page- 5 - Appendix 4.doc 3/11/2005 Figure 2. Badin Dam (The Narrows), Yadkin River. Pennington and Associates, Inc Page- 6 - Appendix 4.doc 3/11/2005 Figure 3. Tuckertown Dam, Yadkin River. Pennington and Associates, Inc Page- 7 - Appendix 4.doc 3/11/2005 Figure4. High Rock Dam, Yadkin River. MATERIALS AND METHODS On July 24, 2003 two transects were established in each tailwater by personnel from Normandeau Associates, Inc. and agency personnel and interested participants from the Fish and Aquatics IGA. The transect locations were established and GPS readings taken for future sampling. The GPS locations are listed in Table 1. Along each transect two divers collected all freshwater mussels (-10 ft wide) and returned them to shore for identification and enumeration. Collections were also made along the banks, islands, back channel and any other available habitats. Dr. Paul Parmalee verified all species taken along the transect and special collections. Mussel searches were conducted each season by divers swimming along the length of each transect line (length dependent on the wetted width of each tailwater at time of sampling). Divers searched at least two meters upstream and downstream of each transect line. Additionally, mussel searches were conducted along the shoreline of each tailrace looking for mussel shells and by having divers search in areas identified by participants as good mussel habitat that were not located along a transect line. Any live RTE mussel species located during these searches were identified, returned to where it was found (if it was removed from the water), and its location recorded with GPS. The locations of any relic mussel shells were also recorded and the shells collected and identified. Scientific and common names of mussels follow those provided by the American Fisheries Society (1988). Also, along each transect three -2m2 benthic macroinvertebrate samples were taken at 25%, 50% and 75% of the distance along each transect for a total of six at each tailwater during each date. An underwater airlift device was used to collect the macroinvertebrate samples (-2m2 sample size) at each station along the transect line. The macroinvertebrate samples were preserved on-site with formalin and returned to Pennington and Associates, Inc. laboratory for storing and identification of species Pennington and Associates, Inc Page- 8 - Appendix 4.doc 3/11/2005 LABORATORY METHODS In the laboratory, the samples were cataloged by assigning a Pennington & Associates, Inc. (PAI) identification number. After cataloging, the samples were rinsed through a 120- micron mesh screen and organisms were removed from the detritus under 5X magnification. The organisms were transferred to 85% ethanol after removal from the debris. The individuals were identified to the lowest practical taxonomic level (species level in most cases) using available keys and counted. Identifications were made with a stereomicroscope (7X to 60X). Slide mounts were made of the chironomids, simuliids, oligochaetes and small crustaceans. For slide-mounted specimens, identifications were made with a compound microscope. The chironomids, simuliids, and oligochaetes were first cleared for 24 hours in cold 10% KOH. Temporary mounts were made in glycerin and the animals returned to 80% ethanol after identification. When permanent mounts were required, the organisms were transferred to 95% ethanol for 30 minutes and mounted in euperol. All species were retained in individual vials in 85% ethanol (or on glass slides if mounted) labeled by PAI number and pertinent project information, and will be archived for a minimum of 5 years. Pennington and Associates, Inc Page- 9 - Appendix 4.doc 3/11/2005 SUBSTRATE DETERMINATION A classification of substrate based on the size scale proposed by Wentworth (Compton 1962) was used to make field observations of substrate type at each sampling location. A visual proportional estimate of the three dominant substrate types was recorded. This classification of sediments is by grain diameter as follows: Diameters Approximate Inch Name of Loose Equivalents Aggregate >256 mm >10 inch Boulder 64 to 256 mm 2.5 to 10 inch Cobble 2 to 64 mm 0.08 to 2.5 inch Gravel 1/16 to 2 mm 0.002 to 0.08 inch Sand 1/256 to 1/16 mm 0.00015 to 0.002 inch Silt <1/256 mm <0.00015 inch Clay COMMUNITY STRUCTURE MEASURES The benthic macroinvertebrate communities at each location in the four tailwaters were assessed and compared using taxa richness, percent contribution of dominant taxon, EPT index, Jaccards Coefficient, Percent Similarity and modified Hilsenhoff Biotic Index. Community similarity between sites was measured by Jaccards Coefficient and Percent Similarity. Jaccards Coefficient is calculated as: Jaccards Coefficient = C 31+32-(- Where S = the number of species in each community and C = the number of species common to both communities. Pennington and Associates, Inc Page- 10 - Appendix 4.doc 3/11/2005 Percent Similarity, for a two-community comparison, is calculated as follows: The number of individuals of each species is calculated as a fractional portion of the total community. The number of individuals for species i in community 1 is compared to the number of individuals for species i in community 2. The lower of the two is tabulated. This procedure is followed for each species. The tabulated list (of the lower of each pair of values) is summed. The sum is defined as the Percent Similarity of the two communities. The percent contribution of the numerically dominant taxon to the total number of organisms in the community is a rough measure of community balance at the lowest possible taxonomic level (Plafkin et al. 1989). A community that is dominated by a few species may be under environmental stress. The total number of species within the pollution sensitive groups Ephemeroptera, Plecoptera, and Trichoptera (EPT) is generally considered a measure of water quality and is listed as the EPT Index (Plafkin et al. 1989). The EPT Index generally increases with increasing water quality. In North Carolina, the EPT index was historically used to assign a biological classification of Excellent, Good, Good/Fair, Fair and Poor. The EPT Index score for piedmont areas for a standard collecting effort in North Carolina was as follows: BIOCLASSIFICATION Excellent Good Good-Fair Fair Poor SEPT PIEDMONT >31 24-31 16-23 8-15 0-7 BIOTIC INDEX After a two-year study of 53 Wisconsin streams, Hilsenhoff (1982) proposed using a biotic index of arthropod populations as a rapid method for evaluating water quality. In 1987, Hilsenhoff expanded and improved his biotic index to more accurately detect subtle organic and nutrient pollution changes in water quality. To calculate the Hilsenhoff Biotic Index, species are assigned pollution tolerance values of 0 to 10. A value of 0 is assigned to species found only in unaltered streams of very high water Pennington and Associates, Inc Page- 11 - Appendix 4.doc 3/11/2005 quality, and a value of 10 is assigned to species known to occur in severely polluted or disturbed streams. Intermediate values are assigned to species that occur in streams with intermediate degrees of pollution or disturbance. Where species cannot be identified, genera are assigned values instead. The Hilsenhoff Biotic Index is calculated from the formula: n, a, B.I. = N where ni is the number of individuals of each species, ai is the tolerance value assigned to that species and N is the total number of individuals in the sample (Hilsenhoff 1982). The index is an average of tolerance values (pertaining to tolerance of organic enrichment). According to Hilsenhoff (1987) the calculated Biotic Index values in Wisconsin streams reflect the following: Biotic Index Water Quality Degree of Organic Pollution 0.00 -3.50 Excellent No apparent organic pollution 3.51 -4.50 Very Good Possibly slight organic pollution 4.51 -5.50 Good Some organic pollution 5.51 -6.50 Fair Fairly significant organic pollution 6.51 -7.50 Fairly Poor Significant organic pollution 7.51 -8.50 Poor Very significant organic pollution 8.51 -10.00 Very Poor Severe organic pollution To calculate the biotic index for the aquatic benthic macroinvertebrate populations of the Yadkin River, the investigators used tolerance values provided in North Carolina Department of Environment, Health and Natural Resources, Division of Environmental Management Water Quality Section, Standard Operating Procedures Biological Monitoring, Environmental Sciences Branch Ecosystems Analysis Unit, Biological Assessment Group, 2003 (North Carolina, Department of Environment, Health and Natural Resources 2003). Hilsenhoff s values were used when North Carolina values were not available. North Carolina provides historical water quality classifications for EPT and Biotic Index values based on three geographic regions (mountains, piedmont and coastal). The Yadkin River Pennington and Associates, Inc Page- 12 - Appendix 4.doc 3/11/2005 is a headwater tributary to the Pee Dee River in North Carolina piedmont region. North Carolina's historical scoring criteria for water quality assessment using the standard qualitative method is as follows: EPT Values NC Biotic Index (Piedmont)Water Quality >31 < 5.19 Excellent 24-31 5.19-5.78 Good 16-23 5.79-6.48 Good - Fair 8-15 6.49-7.48 Fair 0-7 > 7.48 Poor North Carolina Department of Environment and Natural Resources currently places equal weight to the NC Biotic Index value and EPT taxa richness value in assigning a final bioclassification to standard qualitative samples. The following table should be used to determine the scores for EPT taxa richness values and Biotic Index values for all standard qualitative samples: Score BI Values EPT Values Mt P CA Mt P CA 5 <4.00 <5.14 <5.42 >43 >33 >29 4.6 4.00-4.04 5.14-5.18 5.42-5.46 42-43 32-33 28 4.4 4.05-4.09 5.19-5.23 5.47-5.51 40-41 30-31 27 4 4.10-4.83 5.24-5.73 5.52-6.00 34-39 26-20 22-26 3.6 4.84-4.88 5.74-5.78 6.01-6.05 32-33 24-25 21 3.4 4.89-4.93 5.79-5.83 6.06-6.10 30-31 22-23 20 3 4.94-5.69 5.84-6.43 6.11-6.667 24-29 18-21 15-19 2.6 5.70-5.74 6.44-6.48 6.68-6.72 22-23 16-17 14 2.4 5.75-5.79 6.49-6.53 6.73-6.77 20-21 14-15 13 2 5.80-6.95 6.54-7.43 6.78-7.68 14-19 10-13 8-12 1.6 6.96-7.00 7.44-7.48 7.69-7.73 12-13 8-9 7 1.4 7.01-7.05 7.49-7.53 7.74-7.79 10-11 6-7 6 1 >7.05 >7.53 >7.79 0-9 0-5 0-5 Biotic Index corrections for non-summer data: Summer = Jun-Sept, Fall = Oct-Nov, Winter = Dec-Feb, Spring = Mar-May Fall Winter Spring Mountain Correction +0.4 +0.5 +0.5 Piedmont Correction +0.1 +0.1 +0.2 Costal A Correction +0.2 +0.2 +0.3 Pennington and Associates, Inc Page- 13 - Appendix 4.doc 3/11/2005 The Biological Assessment Unit has in prior years (1983-1996) used EPT abundance (EPT N) values in evaluating water quality impacts without formal quantification of criteria. EPT abundance is the sum of the abundance values for all EPT taxa in a sample, where Rare = 1, Common = 3, and Abundant = 10. EPT N allows differentiation of situations where intolerant groups are simply present from situations where intolerant groups are simply present from situations where healthier (more abundant) populations exist in a stream. A rounding approach is applied when the North Carolina Biotic Index and the EPT scoring differs by exactly one bioclassification producing a final score midway between two ratings. When deciding between more than one bioclassification, use the EPT abundance value criteria listed below and round down if the EPT N is less than the value and round up if it is equal to or above the value. Example: When comparing from a Piedmont stream, and the BI score = 5, but the EPT score =4. Round down (to Good) if EPTN < 135. Bioclassification Score (Mountains) MT (Piedmont) P (Coastal) CA Excellent (5) vs. Good (4) 191 135 108 Good (4) vs. Good-Fair (3) 125 103 91 Good-Fair (3) vs. Fair (2) 85 71 46 Fair (2) vs. Poor (1) 45 38 18 PHYSICAL AND CHEMICAL PARAMETERS The physical and field chemical parameters measured in the field included pH, dissolved oxygen, temperature, conductivity, turbidity (visual), stream width and depth. PH was determined at each station with a Fisher Accument Field pH Meter. Dissolved oxygen was determined with an YSI model 51 Dissolved Oxygen Meter. Temperature was taken with a field centigrade thermometer. A LaMotte Conductivity Meter was used to measure conductivity Pennington and Associates, Inc Page- 14 - Appendix 4.doc 3/11/2005 RESULTS AND DISCUSSON Physical characteristics of each tailwater and specific characteristics of the transects are presented in Table 2. Water quality parameters taken at each tailwater location during each of the three seasons are found in Table 3. A summary of freshwater mussels taken from the four tailwaters are listed in Table 4 while all data for each of the three seasons are found in the Appendix, Tables IA, 2A and 3A. Table 5 contains a summary list of benthic macroinvertebrates taken from each tailwater for each season using an uplift device. All benthic macroinvertebrate sample data taken with the uplift device are found in the Appendix, Table 4A. Dominant benthic macroinvertebrate species for each tailwater and date are presented in Table 6. Cluster analyses of the tailwaters and dates using species shared are shown in Figure 5 (Figure IA, Appendix). A similar comparison using percent similarity is shown in Figure 6 (Figure 2A, Appendix). Falls Dam (Photo 1) is the most downstream of the four dams studied. The study transects were situated at approximately 400 feet (T1) and 1000 feet (T2) from the dam (Table 2). These two transects were placed in what was considered riverine habitat (flow and depth). Transect 1 was 976 feet wide while Transect 2 was 671 feet. The depth of Transect 1 was between 4 to as deep as 20 feet. Transect 2 was from 4 to 8 feet deep. The substrate along both transect was mostly boulders and cobble with mixed lenses of gravel. There was a higher percentage of gravel on the descending left bank and in the channels behind both islands (adjacent to left and right banks). The water was always clear during each of the three visits. The pH ranged from a low of 6.26 during the June 2004 visit to a high of 7.33 in the September 2003 visit. Dissolved oxygen was lowest in September at 6.28 mg/l and highest (7.11 mg/1) in November (Table 3). Conductivity ranged from 50 to 95,us. As expected, temperature was highest in September (26.6 °C) and lowest in November (18.2 °C). The Narrows (Badin) Dam (Photo 2) is the third most downstream of the four dams surveyed. The tailwater channel is very narrow with both Transects 1 and 2 only about 300 feet wide (Table 2). Transect 1 was situated at about 700 feet downstream of the dam while Transect 2 was at - 1,700 feet. There was also a shallow overflow channel along the descending left bank which flowed back into the main stem of the river just downstream of Transect 2. The water depths along Transect 1 were from 4 to as much as 20 feet. Transect 2 was mostly 4 feet or less Pennington and Associates, Inc Page- 15 - Appendix 4.doc 3/11/2005 with a deeper zone along the descending right bank of as much as 10 feet deep. The substrate was primarily boulders and cobbles with small amounts of gravel and silt. As with the Falls Dam tailwater, the water in the Narrows Dam Tailwater was also clear during each visit. The lowest pH (6.0), and slightly acidic value, was found in June 2004 while the September 2003 (7.4) value was near neutral (Table 3). Dissolved oxygen levels ranged from 6.44 mg/l in September 2003 to 7.52 mg/l in June 2004. Conductivity was 100,us or less during each period. Temperature ranges were similar to those found in Falls Dam Tailwater. Tuckertown Dam (Photo 3) is the second dam in the four dam series within the Yadkin River study. The river channel in the tailwater is the widest of the four (Table 2). Transect 1 -900 feet downstream of the dam was 843 feet wide while Transect 2, approximately 1,300 feet downstream of the dam, was 1,188 feet wide. Channel depth ranged from 2 to 8 feet at Transect 1 and 2 to 12 feet at Transect 2. The substrate of the tailwater was primarily bedrock and boulders covered with silt at Transect 1 while Transect 2 had mud on bedrock (0-135 feet) along the descending right bank with boulders, cobble and silt on bedrock the primary substrate for the remaining length of Transect 2. The water in the tailwater was slightly turbid during each visit. The pH was near neutral in September and June. The meter probe malfunctioned in November and no reading was taken during that period. Dissolved oxygen levels were reduced in June 2004 at 3.40 mg/l when compared to the readings in September (6.29 mg/1) and November (7.68 mg/1). Conductivity was elevated (150 us) in June when compared to the November (90) and September (80) periods. Temperatures were similar in September and June and reduced in November. High Rock Dam (Photo 4) is the most upstream dam in the project area. Transect 1 was situated approximately 500 feet downstream of the dam while Transect 2 was at approximately 900 feet downstream (Table 2). Transect 1 was 455 feet wide and ranged from 2 to 10 feet deep. Transect 2 was 675 feet wide and 3 to 10 feet deep. The substrate along Transect 1 was primarily boulders with lesser amounts of cobble and gravel, all covered with silt. Transect 2 had a substrate of mud from the descending right bank to 100 feet in the channel and boulders with lesser amounts of coble and gravel covered with silt for the remaining length of the transect. The water was slightly turbid during each visit, usually with a visibility of less than 3 feet. PH ranged from 6.38 in June to 7.28 in September. Dissolved oxygen, as with Tuckertown, was reduced in June (4.77 mg/1) and highest in September (7.04 mg/1) and November (8.04 mg/1). Pennington and Associates, Inc Page- 16 - Appendix 4.doc 3/11/2005 Conductivity was similar (70-105 us) throughout the three seasons. Temperatures were similar in September and June and reduced in November. FRESHWATER MUSSELS There were 7 species of unionid mussels, 3 species of fingernail clams (Table 5), and 1 species of Asiatic clam (Corbicula fluminea) taken in the four tailwaters. Anodonta implicata (alewife floater) and Lampsilis radiata (Photo 5) (Eastern lamp mussel) are considered state threatened (Bogan 2002). The species listed by the field investigator as Elliptio cf. lancolata (Photo 6) is according to Bogan (personal communication) an undescribed Ellipito which he describes as the Pee Dee Lance. Elliptio lancolata (Yellow lance) is considered a state endangered species. Villosa delumbis (Photo 7) (Eastern creek shell) is considered significantly rare by the North Carolina Heritage Program. No federally listed species were taken during the survey. All other species taken during this study are considered stable (Bogan 2002). Even though it is not a mussel, there is also a large introduced viviparid snail Cipangopaludina chinensis Chinese mystery snail (Photo 8), which occurs in large numbers in the High Rock Dam Tailwater. This species is a food item in Southeast Asia and considered an exotic in North America. The Falls Dam Tailwater yielded 7 species of unionid mussels and over 575 live individuals (Table 4, Appendix Tables IA, 2A and 3A) after more than 70-man hours search efforts during the three seasons. Elliptio complanta (Photo 9) was the dominant species while Lampsilis radiata and Elliptio cf lancolata were secondary in abundance (Table 2). The Narrows Dam Tailwater survey produced 6 species and 22 live individuals after more than 50-man hour searches during the three seasons (Table 4). The Eastern Elliptio (Elliptio camplanta) was the most common species in the substrate. The Paper Pondshell (Utterbackia imbecillis) (Photo 10) was the only species of freshwater mussel found in both Tuckertown Dam and High Rock Dam tailwaters. Approximately 50-man hours were expended at each of the two tailwaters searching for mussels. This species is usually found in a mud, sand and/or silt substrate in backwaters, ponds and impoundments (Bogan 2002). Pennington and Associates, Inc Page- 17 - Appendix 4.doc 3/11/2005 BENTHIC MACROINVERTEBRATES A minimum of 99 species of benthic macroinvertebrates was taken throughout the four tailwaters in the Yadkin River Project area by SCUBA using the uplift sampling device (Table 5, Appendix Table 4A). There were 6 phyla, 24 orders and 41 families (16 insect families) represented by the species found in the tailwaters (Table 5). The tailwaters in Falls Dam during the June period had the highest number of species with 53, followed by 48 from Narrows Dam Tailwater in September, 46 from High Rock in June, 44 from Tuckertown in June, 43 from High Rock in November and 42 from Falls Dam in September (Table 5). High Rock Dam Tailwater in September yielded the least with 29. The highest numbers of individuals per sample were found during the June period in the Narrows Dam Tailwater (12,008/12m2) and Falls Dam Tailwater (10,172/12m2). The lowest 2 2 numbers were found in November with, 1420/12M in Falls Dam and 1333/12M in Narrows Dam. Falls Dam Tailwater benthic fauna was dominated by the Asiatic clam Corbicula fluminea (27.7% in September, 48% in November and 11.0% in June), the isopod Caecidotea sp. (11.1%) in September, 6.8% in November and 17.3% in June), and the fingernail clam Musculium transversum (15.1% in September in September and 6.2% in November) (Table 6). The highest number of species was found in June (53) while the least was taken in November (34). The EPT and Biotic Index values for the Falls Dam Tailwater indicates a benthic fauna existing under mostly "Poor' water quality conditions in June and no better than "Fair" water quality conditions in September and November (Table 5). The benthic fauna in the Narrows Dam Tailwater was represented by a minimum of 48 species in September, 33 in November and 37 in June (Table 5). The midge Rheotanytarsus sp. (22.5%) was dominant in September while Corbicula fluminea (43.5%) was dominant in November and the isopod Caecidotea sp. (16.3%) in June (Table 6). Other species that were abundant during the September period included Caecidotea sp. (17.0%), Musculium transversum (9.6%), Dugesia tigrina (9.3%) and Cyrnellus fraternus (7.3%). The isopod Caecidotea sp. (17.9%) was abundant during the November period. Also, during the June survey Corbicula fluminea (9.3%), Slavina appendiculata (14.6%), Lumbriculidae (10.0%), Hyalella azteca Pennington and Associates, Inc Page- 18 - Appendix 4.doc 3/11/2005 (6.8%) and Cricotopus sp. (15.0%) were abundant in the fauna. The EPT and Biotic Index values were reflective of primarily "Poor" water quality condition in June and "Fair" during the September and November periods (Table 5). The Tuckertown Tailwater benthic fauna had a minimum of 44 species in June, 39 in November and 38 in September (Table 5). The fingernail clam Musculium transversum was dominant in September (38.2%) and November (53.2%) and abundant in June (18.5%). The isopod Caecidotea sp. (29.7%) was dominant in the June period. In September, the midge Dicrotendipes simpsoni (24.5%), Caecidotea sp. (10.0%) and the snail Menetus dilatatus (6.3%) were abundant. The isopods Caecidotea sp. (13.8%), the midge Glyptotendipes sp. (9.2%), and the flatworm Dugesia tigrina (6.2%) were common during the November period. The EPT and Biotic Index Values indicate a benthic fauna existing under mostly "Poor" water quality conditions for all three seasons. The High Rock Tailwater consisted of a benthic fauna with a minimum of 46 species in June, 43 in November and 29 in September. The dominant species in the High Rock Tailwater included Musculium transversum (43.7% in September and 35% in June) and Caecidotea sp. (28.8%) in November. During September, Dicrotendipes simpsoni (22.3%), Caecidotea sp. (12.6%), and Dugesia tigrina (7.6%) were abundant. Musculium transversum (28.3%), Dugesia tigrina (12.5%) and Dicrotendipes simpsoni (11.7%) were also abundant during the November period. The midge Glyptotendipes sp. (9.2%), the worm Slavina appendiculata (9.1%) and Caecidotea sp. (6.8%) were common species during the June period. The benthic fauna in the High Rock Tailwater indicates a fauna existing under "Poor" water quality conditions for all seasons studied. A comparison of the tailwater fauna by date using species shared is presented in Figure 5. The most similar in terms of species shared are the Narrows Dam Tailwater in September and Tuckertown Dam Tailwater in November. Benthic populations which form a secondary cluster with the two previously listed tailwaters and dates include Tuckertown Dam Tailwater in June and High Rock Dam Tailwater in September. Falls Dam in June was the least similar and clusters last. All other locations and dates were fairly similar and clustered at a similar distance (Figure 5). Similar comparisons of the tailwaters and dates using species shared with a density component are shown in Figure 6. High Rock Dam Tailwater in June and Falls Dam Tailwater Pennington and Associates, Inc Page- 19 - Appendix 4.doc 3/11/2005 in September were the most similar and clustered first. The Tuckertown in June and November were similar at a greater distance and formed secondary clusters with Narrows Dam (September) and Falls Dam (November). Falls Dam (June), High Rock Dam (September), Narrows Dam (June) and Tuckertown Dam (September) also formed a distinct cluster. High Rock Dam and Narrows Dam tailwaters in November clustered at the greatest distance. High Rock (June) and Falls Dam (September) which formed the first distinct cluster, clustered last to all other tailwater populations. Pennington and Associates, Inc Page- 20 - Appendix 4.doc 3/11/2005 Table 1. Transect Locations, Yadkin River, 2003. Falls Dam Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°23'45.4" W80°04'22.5" N35°23'36.7" W80°04'28.1" 2 N35°23'36.2" W80°04' 15.9" N35°23'31.2" W80°04'21.3" Badin Dam (The Narrows) Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°25'06.6" W80°05'28.0" N35°25'04.5" W80°05'30.6" 2 N35°24'54.6" W80°05'22.8" N35°24'54.4" W80°05'27.3" Tuckertown Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°29'05.2" W80°10'23.2" N35°28'58.3" W80°10'38.5" 2 N35°29'02.2" W80°10'18.6" N35°28'55.1" W80°10'24.5" High Rock Tailwater Transect No. East Bank West Bank Lat. Long. Lat. Long. 1 N35°35'58.5 W80°13'59.1" N35°35'55.4" W80°14'04.8" 2 N35°35'55.3" W80°13'57.2" N35°35'52.0" W80°14'03.8" Pennington and Associates, Inc Page- 21 - Appendix 4.doc 3/11/2005 Table 2. Physical Characteristics, Yadkin River Project. Falls Dam Narrows Dam Tuckertown High Rock Distance from Dam T1 (ft) -400 -700 -900 -500 Width ft T1 976 -300 843 455 Distance from Dam T2 (ft) 1000 1,700 1,300 -900 Width (ft) T2 671 -300 1,188 675 Depth ft T1 4-20 4-20 2-8 2-10 Depth (ft) T2 4-8 4-10 2-12 3-12 0'-200' bedrock and boulders Boulders few Boulders ' ' , Substrate T1 cobble and 200 -300 Bedrock, cobble and Boulders with boulders silt gravel covered gravel , cobble, gravel with silt and silt 0-135' mud on bedrock 135'- 250' boulders 0-100' mud Boulders ' ' ' ' Boulders , cobble with 500 -750 100 -675 , boulders few boulders few Substrate T2 cobble and lesser amounts cobble 750'- cobble and gravel of gravel and ' 1188 gravel covered silt bedrock, with silt cobble with silt Pennington and Associates, Inc Page- 22 - Appendix 4.doc 3/11/2005 Table 3. Chemical Characteristics, Yadkin River Project. September 2003 November 2003 June 2004 Falls Dam Narrows Dam Tucker Town High Rock Falls Dam Narrows Dam Tucker Town High Rock Falls Dam Narrows Dam Tucker Town High Rock PH (standard units) 7.33 7.40 7.04 7.28 6.75 6.70 a a 6.26 6.00 6.80 6.38 Dissolved oxygen (mg/1) 6.28 6.44 6.29 7.04 7.11 7.32 7.68 8.04 6.62 7.52 3.40 4.77 Temperature (OC) 26.6 26.6 25.6 23.0 18.2 17.4 14.9 15.10 22.6 20.6 26.0 25.5 Conductivity (us) 50 60 80 70 90 100 90 70 95 98 150 105 Total Dissolved Solids (mg/1) 30 30 40 30 40 50 40 40 a meter malfunction Pennington and Associates, Inc. Page - 23 - Appendix 4.doc 3/11/2005 Table 4. Summary of Mollusca taken from Yadkin River, 2003-2004 Species Falls Dam Tailwater Badin Dam (Narrows) Tailwater Tuckertown Tailwater High Rock Tailwater Anodonta implicata Alewife floater R 1 Elliptio complanta Eastern Elliptio 328 16 Elliptio cf. lanceolata Pee Dee Lance 113 1 Lampsilis radiata Eastern lam mussel 117 R Pyganodon cataracts Eastern floater 1 2 Utterbackia imbecillis Paper and shell 8 2 4 1 Villosa delumbis Eastern creekshell 8 Total No. Of Unionidae Species 7 6 1 1 Total No. Of Individuals 575 22 4 1 Corbicula fluminea A A A A Ci an o alucdinea chinensis 231 Chinese mystery snail R = represented by relics only A= abundant Pennington and Associates, Inc Page- 24 - Appendix 4.doc 10/22/2004 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL COELENTERATA Hyd rozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Pennington and Associates, Inc Appendix 4.doc 1 1 2 12 7.2 117 294 245 685 75 50 608 717 513 250 29 150 1 10 2 1 5.1 FC 1 2 1 5 1 1 1 6.1 FC 542 207 18 3 682 580 205 28 1118 1122 64 119 "8 FC 13 5 5.7 FC 114 2 44 5 58 "8 FC 307 306 2461 3921 89 72 5239 1610 623 165 349 1406 6.5 FC 6 1 Page- 25 - 10/22/2004 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL Gastropoda Mesogastropoda Hydrobiidae "8 SC Amnicola limosa 4 6 49 4 10 30 5 106 17 Viviparidae Cipangopaludina chinensis 2 12 Basommatophora Ancylidae SC Ferrissia rivularis 6.6 SC 49 19 198 2 33 14 26 8 9 2 Physidae Physella sp. 8.8 CG 12 35 20 17 27 12 42 43 839 404 35 58 Planorbidae "6 SC Helisoma anceps 6.2 SC 1 15 1 Menetus dilatatus 8.2 SC 2 24 409 62 2 1 11 123 227 163 26 180 ANNELIDA Oligochaeta "1 CG Tubificida Naididae "8 CG 14 19 194 53 8 72 Dero sp. 10.0 CG 3 3 37 3 10 80 1 271 31 Nais sp. 8.9 CG 1 4 Nais communis 8.8 CG 31 24 43 51 76 180 2 25 Nais bretscheri "6 CG 4 Pristina sp. 9.6 CG 1 Pristina leidyi 9.6 CG 2 Pristinella sp. 7.7 CG 1 Ripistes parasita 2 FC 90 87 2 1 Pennington and Associates, Inc Page- 26 - Appendix 4.doc 10/22/2004 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w. h. c. Branchiura sowerbyi Quistadrilus multisetosus Lumbriculida Lumbriculidae Hirudinea Erpobdellidae Erpobdella punctata Rhynchobdellida Glossiphoniidae Batrachobdella phalerata Helobdella sp. Helobdella stagnalis Helobdella triserialis Placobdella translucens ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Pennington and Associates, Inc Appendix 4.doc 7.1 CG 3 193 1758 25 364 5.3 CG 1 9.4 CG 3 16 156 403 7.1 CG 2 7 98 30 11 22 7 3 266 321 60 23 9.5 CG 116 16 7.1 CG 6 1 40 1 8 8.3 CG 47 57 3.9 CG 2 7.0 CG 37 9 1 33 17 1 526 1197 8 "8 P 4 46 13 2 81 2 13 26 27 8 "8 P 1 2 8 14 5 6 20 25 6 P 2 "8 P 6 20 1 4 27 10 7.6 P 1 "6 P 9 70 5 8.6 P 72 41 53 4 52 149 9.2 P 1 2 27 4 5 40 24 36 115 9.0 P 2 5 5 1 1 4 Page- 27 - 10/22/2004 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL Daphnidae Daphnia sp. Sididae Sida crystillina Copepoda Cyclopoida Isopoda Asellidae Caecidotea sp. Amphipoda Crangonyctidae Crangonyx sp. Talitridae Hyalella azteca Decapoda Cambaridae Insecta Collembola Ephemeroptera Caenidae Caenis sp. Heptageniidae Stenacron interpunctatum Stenonema sp. Tricorythidae Tricorythodes sp. Pennington and Associates, Inc Appendix 4.doc 1 15 1 20 9 9 2 5 1 1 5 10 "8 S H 9.1 CG 225 542 646 1130 97 238 1356 1641 1762 1952 557 272 7.9 CG 1 7.8 CG 70 123 22 7.5 1 12 1 1 2 158 93 295 1 826 813 11 7 2 1 "7 CG 7.4 CG 1 3 11 "4 SC 6.9 SC 4 1 "4 SC 1 9 1 4 58 10 5 5.1 CG Page- 28 - 10/22/2004 1 1 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL Odonata Coenagrionidae Argia sp. Corduliidae Neurocordulia sp. Gomphidae Gomphus sp. Neuroptera Sisyridae Trichoptera Hydropsychidae Cheumatopsyche sp. Hydroptilidae Hydroptila sp. Leptoceridae Ceraclea sp. Polycentropodidae Cyrnellus fraternus Neureclipsis sp. Diptera Chaboridae Chaoborus punctipennis Chironomidae Ablabesmyia mallochi Ablabesmyia rhamphe gp. Chironomus sp. Pennington and Associates, Inc Appendix 4.doc "9 P 8.2 P 2 1 "5 P 5.0 1 5.8 P 1 1 1 "4 FC 6.2 FC 68 146 1 "4 PI 1 6.2 PI 10 12 9 1 11 30 12 1 CG 2 CG 1 "6 FC "8 FC 134 231 32 7 55 13 47 38 27 4.2 FC 1 40 8.5 P 8 10 19 35 62 3 211 124 13 49 7.2 P 20 1 "6 P 50 44 17 2 5 4 438 225 1 14 9.6 CG 14 15 2 19 Page- 29 - 10/22/2004 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL Clinotanypus sp. Coelotanypus sp. Conchapelopia sp. Cricotopus sp. Cricotopus bicinctus Cryptochironomus sp. Diamesa sp. Dicrotendipes lucifer Dicrotendipes neomodestus Dicrotendipes simpsoni Dicrotendipes sp. Endochironomus sp. Einfeldia natchitocheae Eukiefferiella claripennis gp. Glyptotendipes sp. Microtendipes pedellus gp. Nanocladius distinctus Orthocladius (Euorthocladius) sp. Parachironomus sp. Phaenopsectra sp. Parakiefferiella sp. Polypedilum flavum Polypedilum illinoense Procladius sp. Pseudochironomus sp. Rheotanytarsus sp. Pennington and Associates, Inc Appendix 4.doc P 8 P 8.4 P 1 "7 CG 157 8.5 CG 61 6 5 6.4 P 8.0 CG 1 8.0 CG 1 8.1 CG 12 4 10.0 CG 10 55 157 8.1 CG SH 5.6 CG 1 9.1 FC 3 24 22 5.5 CG 7.1 CG 6 28 40 "4 CG 2 25 9.4 CG 1 16 30 6.5 SC 5.4 CG 4.9 S H 9.0 SH 5 9.1 P 1 5.4 CG 5 5.9 FC 137 715 10 52 116 376 1806 2 9 460 243 29 41 5 1 10 216 166 9 1999 3 21 393 669 6 36 7 1 1 108 0 576 1 16 912 276 14 35 20 5 271 6 12 107 5 22 1 1 59 110 18 133 165 3 1 2 1 2 5 1 2 3 20 13 1 15 77 20 Page- 30 - 10/22/2004 1 36 2 9 7 1 2 17 31 3 17 122 3 8 1 97 367 3 5 3 214 10 Table 5. Benthic Macroinvertebrates Collected from Yadkin River 2003-2004 (No./-2m2) SPECIES T.V. F.F.G. Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Sep-03 Sep-03 Sep-03 Sep-03 Nov-03 Nov-03 Nov-03 Nov-03 Jun-04 Jun-04 Jun-04 Jun-04 TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL TOTAL Stenochironomus sp. 6.5 SH 1 5 Tanytarsus sp. 9.2 FC 1 177 35 39 9 Tribelos fuscicorne 6.3 CG 1 316 Tribelos sp. 6.3 CG 1 Tvetenia bavarica gp. 3.7 CG 1 Xenochironomus xenolabis 7.1 P 4 27 21 5 2 8 20 18 5 Empididae 7.6 P Hemerodromia sp. "6 P 2 1 Simuliidae "6 FC Simulium sp. 6.0 FC 2 Tipulidae Tipula sp. 7.3 SH 1 TOTAL NO. OF ORGANISMS 2030 3179 6445 8981 1420 1333 9856 5695 10172 12008 1878 4000 TOTAL NO. OF TAXA 42 48 38 29 34 33 39 42 53 37 44 46 EPT INDEX 6 5 2 2 4 3 2 5 5 2 1 NC BIOTIC INDEX 7.14 7.33 8.54 8.57 6.94 7.19 8.20 8.38 8.09 7.75 8.65 8.24 Pennington and Associates, Inc Page- 31 - Appendix 4.doc 10/22/2004 Table 6. Dominant (%) Benthic Macroinvertebrate Species, Yadkin River Pro'ect. September 2003 November 2003 June 2004 SPECIES Falls Narrows Tucker High Falls Narrows Tucker High Falls Narrows Tucker High Dam Dam Town Rock Dam Dam Town Rock Dam Dam Town Rock Dugesia tigrina 9.3 7.6 6.2 12.5 Corbicula fluminea 26.7 48.0 43.5 11.0 9.3 Musculium transversum 15.1 9.6 38.2 43.7 6.2 53.2 28.3 18.6 35.2 Physella sp. 8.2 Menetus dilatatus 6.3 Dero sp. 14.1 Slavina appendiculata 14.6 9.1 Lumbriculidae 10.0 Caecidotea sp. 11.1 17.0 10.0 12.6 6.8 17.9 13.8 28.8 17.3 16.3 29.7 6.8 Hyalella azteca 11.1 8.2 6.8 Cyrnellus fraternus 7.3 Cricotopus sp. 15.0 Dicrotendipes simpsoni 24.5 22.3 11.7 Glyptotendipes sp. 9.2 9.2 Rheotanytarsus sp. 22.5 Pennington and Associates, Inc. Page - 32 - Appendix 4.doc 3/11/2005 JACCARD COEFFICIENT STATION fd604 h r604 tt903 nd604 nd1103 fd1103 h r903 tt604 tt 1103 nd903 hr1103 fd903 2.00 1.50 1.00 0.50 0.00 DISTANCE Figure 5. Comparison of Tailwaters (fd = Falls Dam, nd = Narrows Dam, tt = Tuckertown Dam and hi= High Rock Dam) using species shared. Pennington and Associates, Inc. Page - 33 - Appendix 4.doc 3/11/2005 PERCENT SIMILARITY STATION hr1103 nd1103 fd604 h r903 nd604 tt903 fd1103 tt604 tt 1103 nd903 h r604 fd903 1.50 1.13 0.75 0.38 0.00 DISTANCE Figure 6. Comparison of Tailwaters (fd = Falls Dam, nd = Narrows Dam, tt = Tuckertown Dam and hi= High Rock Dam) using species shared with a density component. Pennington and Associates, Inc Page- 34 - Appendix 4.doc 10/22/2004 zr- Photo 1. Falls Dam. Pennington and Associates, Inc Page- 35 - Appendix 4.doc 10/22/2004 Photo 4. High Rock Dam. Pennington and Associates, Inc Page- 36 - Appendix 4.doc 10/22/2004 Photo 3. Tuckertown Dam. Photo 5. Lampsilis radiata. z 4 5 0 60 70 Be so ;w 110 12C 1 0 140 150 -- 0 c 2 7 4 5 f 7 $ 4 i u OFT- a 3 ?`'_? •? ilk r t . i "ol NV 0: y? Photo 6. Elliptio ct lancolata. Pennington and Associates, Inc Page- 37 - Appendix 4.doc 10/22/2004 Photo 7. Villosa delumbis. 11 Photo 8. Cipangopaludina chinensis. Pennington and Associates, Inc Page- 38 - Appendix 4.doc 10/22/2004 Photo 9., Elliptio camplanta. Pennington and Associates, Inc Page- 39 - Appendix 4.doc 10/22/2004 Photo 10. Utterbackia imbecillis. REFERENCES American Fisheries Society. 1988. Common and Scientific Names of Aquatic Invertebrates from the United States and Canada: Mollusks. American Fisheries Society, Special Publication 16, Bethesda, Maryland. 277 pp. Barbor, M.T., J. Gerritsen, B.D. Snyder, and J.B. Stribling. 1999. Rapid Bioassessment Protocols for use in Wadeable Streams and Rivers, Periphyton, Benthic Macroinvertebrates and Fish. EPA 841-B-99-002, Washington, DC. Barbour, M.T., J.L. Plafkin, B.P. Bradley, C.G. Graves, and R.W. Wisseman. 1992. Evaluation of EPA's Rapid Bioassessment Benthic Metrics: Metric Redundancy and Variability Among Reference Stream Sites. Environmental Toxicology and Chemistry, Vol. 11:437- 449. Bogan, A; E. 2002. Workbook and Key to the Freshwater Bivalves of North Carolina Freshwater Mussel Conservation Partnership, Raleigh, NC 101 pp, 10 color plates. Brower, T.E. and J.H. Zar. 1984. Field and Laboratory Methods for General Ecology. Second Edition. W.C. Brown, Dubuque. 226 pp. Compton, Robert R. 1962. Manual of Field Geology. John Wiley and Sons, Inc., New York, NY. 378 pp. Hilsenhoff, William L. 1982. Using a biotic index to evaluate water quality in streams. Department of Natural Resources, Madison, Wisconsin, Technical Bulletin No. 132: 22 pp. Hilsenhoff, William L. 1987. An improved biotic index of organic stream pollution. The Great Lakes Entomologist, Vol. 20(1):31-39. Howmiller, R.P. and M.A. Scott. 1977. An environmental index based on relative abundance of oligochaete species. JWPCF 49:809-815. Isom, B.G. and C. Gooch. 1986. "Rational and Sampling Designs for Freshwater Mussel Unionidae in Streams, Large Rivers, Impoundments, and Lakes" Rational for Sampling and Interpretation of Ecological Data in the Assessment of Freshwater Ecosystems, ASTM STP 894, B.G. Isom, ed., American Society for Testing and Materials, Philadelphia, 1986: 46-59. Pennington and Associates, Inc Page- 40 - Appendix 4.doc 10/22/2004 Johnson, R.I. 1970. The Systematic and Zoogeography of the Unionidae (Mollusca: Bivalvia) of the Southern Atlantic Slope Region. Bulletin of the Museum of Comparative Zoology. 140 (6): 263-449. Klemm, D.J., P. A. Lewis, F. Falk and J.M. Lazorchak. 1990. Macroinvertebrate Field and Laboratory Methods for Evaluating the Biological Integrity of Surface Waters. USEPA/600/4-901030, Cincinnati, Ohio. 256 pp. Merritt, R.W. and K. W. Cummins. 1996. An Introduction to the Aquatic Insects of North America, Third Edition. Kendall/Hunt Publishing Company, Dubuque, Iowa. 862 pp. North Carolina Department of Environment, Health and Natural Resources. 2003. Standard Operating Procedures Biological for Benthic Macroinvertebrates, Biological Assessment Unit. 44 pp. Parmalee, P.W. and A.E. Bogan. 1988. The Freshwater Mussels of Tennessee. The University of Tennessee Press, Knoxville. 328 pp. Pennington & Associates, Inc. 1994. Standard Operating Procedures for Processing, Identification and Enumeration of Invertebrate Samples. Pennington and Associated, Inc. Unpublished working document, Cookeville, TN 85 pp. Plafkin, J.L., M.T. Barbour, K.D. Porter, S.K. Gross and R.M. Hughes. 1989. Rapid bioassessment protocols for use in streams and rivers: Benthic Macroinvertebrates and Fish. EPA/440/4-89/00/, Washington, D.C. Shackleford, B. 1988. Rapid Bioassessments of lotic Macroinvertebrate Communities: Biocriteria Development. Arkansas Department of Pollution Control and Ecology, Little Rock, Arkansas. 45 pp. Waters, T.E. 1995. Sediment in Streams, Sources, Biological Effects, and Control. American Fisheries Society Monograph 7, Bethesda, Maryland. 251 pp. Weber, Cornelius, Ed. 1973. Biological field and laboratory methods for measuring the quality of surface waters and effluents. U.S.E.P.A. Report No. EPA 670/4-73- 001. Pennington and Associates, Inc Page- 41 - Appendix 4.doc 10/22/2004 APPENDIX Pennington and Associates, Inc Page- 42 - Appendix 4.doc 10/22/2004 JACCARD COEFFICIENT STATION h r604-t2 h r604-t 1 tt604-t2 tt604-t1 nd604-t2 nd604-t1 fd604-t2 fd604-t1 hr903-t1 hr1103-t1 tt 110341 h r110342 tt903-t2 h r903-t2 tt903-t1 tt 110342 fd 1103-t1 nd 1103-t' nd903-t2 nd 1103-t1 fd 110342 fd903-t2 nd903-t1 fd903-t1 2.00 1.50 1.00 0.50 0.00 DISTANCE Figure IA. Comparison of Tailwatertransects (fd = Falls Dam, nd = Narrows Dam, tt = Tuckertown Dam and hr= High Rock Dam) using species shared. Pennington and Associates, Inc Page- 43 - Appendix 4.doc 10/22/2004 PERCENT SIMILARITY STATION tt604-t1 tt604-t2 h r110342 hr1103-t1 h r604-t2 h r604-t 1 tt 110342 tt903-t2 tt 110341 h r903-t2 hr903-t1 tt903-t1 nd903-t2 nd604-t2 nd604-t1 fd604-t1 nd 1103-t2 fd604-t2 nd903-t1 fd903-t2 nd 1103-t1 fd 110342 fd 1103-t1 fd903-t1 2.00 1.50 1.00 0.50 0.00 DISTANCE Figure 2A. Comparison of Tailwater transects (fd = Falls Dam, nd = Narrows Dam, tt = Tuckertown Dam and hr= High Rock Dam) using species shared with a density component. Pennington and Associates, Inc Page- 44 - Appendix 4.doc 10/22/2004 TABLE 1A. MOLLUSCA, YADKIN RIVER, SEPTEMBER 2003. FALLS DAM TAILWATER Rapids behind SPECIES East Bank W. Bank T1 Island T2 Island Live Relic Live Relic Live Relic Live Relic Anodonta implicata 5 Corbicula fluminea A A A A A A A A Elliptio cf lanceolata 6 4 7 5 7 1 7 1 Elliptio complanata 21 9 34 29 23 15 26 9 Lampsilis radiata 11 2 15 11 13 7 1 Pyganodon cataracta 3 Utterbackia imbecillis 1 Villosa delumbis 3 BADIN DAM (THE NARROWS) TAILWATER East Bank SPECIES T1 Island T2 Live Relic Live Relic Live Relic Anodonta implicata 1 4 Elliptio complanata 1 Utterbackia imbecillis 1 Corbicula fluminea A A A A A A TUCKERTOWN TAILWATER SPECIES T1 T2 Live Relic Live Relic Corbicula fluminea A A A A HIGH ROCK TAILWATER SPECIES T1 T2 Shallows Live Relic Live Relic Live Relic Corbicula fluminea A A A A A A Cipangopaludina chinensis 9 1 16 A=abundant Pennington and Associates, Inc. Page - 45 - Appendix 4.doc 3/11/2005 Table 2A. Mollusca, Yadkin River, November 2003 FALLS DAM TAILWATER T1 T2 Species Live Relic Live Relic Elliptio complanata 20 34 4 11 Elliptio cf lanceolata 11 11 15 Lampsilis radiata 22 13 7 5 Pyganodon cataracta 1 2 Utterbackia imbecillis 2 Villosa delumbis 1 2 Corbicula fluminea A A A A BADIN DAM (THE NARROWS) TAILWATER T1 T2 Species Live Relic Live Relic Elliptio complanata 1 6 Elliptio cf lanceolata 1 4 Lampsilis radiata 1 Pyganodon cataracta 1 Utterbackia imbecillis 2 Corbicula fluminea A A A A TUCKERTOWN TAILWATER T1 T2 Species Live Relic Live Relic Corbicula fluminea A A A A HIGH ROCK TAILWATER T1 T2 Species Live Relic Live Relic Corbicula fluminea A A A A Cipangopaludina chinensis 7 A=abundant Pennington and Associates, Inc Page- 46 - Appendix 4.doc 10/22/2004 Table 3A. Mollusca, Yadkin River, June 2004. Falls Dam Tailwater T1 T2 East Bank Island Live Relic Live Relic Live Relic Elliptio complanata 12 12 24 8 164 64 Elliptio cf lanceolata 13 9 17 9 45 14 Lampsilis radiata 8 11 3 30 13 Pyganodon catarcta 1 1 Utterbackia imbecillis 5 Villosa delumbis 8 Corbicula fluminea A A A A A A Badin Dam (The Narrows) Tailwater T1 T2 Shoreline Live Relic Live Relic Live Relic Elliptio complanata 4 10 4 Elliptio cf lanceolata 2 Pyganodon catarcta 1 1 1 Utterbackia imbecillis 1 Corbicula fluminea A A A A A A Tuckertown Tailwater T1 T2 Live Relic Live Relic Utterbackia imbecillis 1 Corbicula fluminea A A A A A A High Rock Tailwater T1 T2 Live Relic Live Relic Corbicula fluminea A A A A A A Cipangopaludina chinensis 198 Pennington and Associates, Inc Page- 47 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls DamTailwater-September 2003 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Oligochaeta 7.2 5.1 FC 6 11 11 28 50 25 14 89 117 1 1 1 6.1 FC 19 82 121 222 97 48 175 320 542 *8 FC 13 13 13 5.7 FC 1 61 27 89 15 10 25 114 *8 FC 12 64 76 229 2 231 307 6.5 FC *8 SC 1 3 4 4 SC 6.6 SC 5 5 28 12 4 44 49 8.8 CG 1 2 3 4 2 3 9 12 *6 SC 6.2 SC 1 1 1 8.2 SC 1 1 2 2 *1 CG Pennington and Associates, Inc Page- 48 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls DamTailwater-September 2003 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Tubificida Naididae Dero sp. Nais sp. Nais communis Nais bretscheri Pristina sp. Pristina leidyi Pristinella sp. Ripistes parasita Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w.h.c. Branchiura sowerbyi Quistradrilus multisetosus Lumbriculida Lumbriculidae Hirudinea Erpobdellidae Erpobdella punctata Rhynchobdellida Glossiphoniidae Batrachobdella phalera Helobdella sp. Helobdella stagnalis Helobdella triserialis Placobdella translucens ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida *8 CG 4 4 6 4 10 14 10.0 CG 3 3 3 8.9 CG 1 1 1 8.8 CG *6 CG 9.6 CG 9.6 CG 7.7 CG 2 FC 7.1 CG 5.3 CG 9.4 CG 7.1 CG 2 2 2 9.5 CG 7.1 CG 8.3 CG 3.9 CG 7.0 CG 5 5 25 2 5 32 37 *8 P 1 2 3 1 1 4 *8 P P *8 P 7.6 P *6 P 8.6 P 9.2 P 9.0 P Pennington and Associates, Inc Page- 49 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls DamTailwater-September 2003 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Isopoda Asellidae Caecidotea sp. Amphipoda Crangonyctidae Crangonyx sp. Talitridae Hyalella azteca Decapoda Cambaridae Insecta Collembola Ephemeroptera Caenidae Caenis sp. Heptageniidae Stenacron interpunctatum Stenonema sp. Tricorythidae Tricorythodes sp. Odonata Coenagrionidae Argia sp. Corduliidae Neurocordulia sp. Gomphidae Gomphus sp. Neuroptera Sisyridae Trichoptera Hydropsychidae Cheumatopsyche sp. Hydroptilidae Hydroptila sp. Leptoceridae Ceraclea sp. Polycentropodidae Cyrnellus fraternus Neureclipsis sp. Diptera Chaboridae Chaoborus punctipennis *8 SH 9.1 CG 13 7.9 CG 7.8 CG 7.5 1 9 83 97 105 23 41 50 9 3 1 128 225 8 20 70 1 1 *7 CG 7.4 CG 1 1 1 *4 SC 6.9 SC 3 3 1 1 4 *4 SC 5.1 CG *9 P 8.2 P 1 1 2 2 *5 P 5.0 5.8 P *4 FC 6.2 FC 41 41 21 4 2 27 68 *4 PI 6.2 PI 6 4 10 10 CG 2 CG *6 FC *8 FC 11 40 48 99 6 10 19 35 134 4.2 FC 1 1 1 8.5 P Pennington and Associates, Inc Page- 50 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls DamTailwater-September 2003 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Chironomidae Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 4 6 Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P 1 Cricotopus sp. *7 CG Cricotopus bicinctus 8.5 CG Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG 6 Dicrotendipes simpsoni 10.0 CG 5 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 2 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudochironomus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC 97 9 Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH 14 24 12 9 5 26 50 1 1 50 7 4 61 61 1 1 1 1 1 1 1 7 4 1 5 12 5 5 5 10 3 3 3 1 3 1 1 1 3 6 2 2 2 1 1 1 2 108 18 7 1 1 1 2 2 4 4 4 29 137 1 1 1 Pennington and Associates, Inc Page- 51 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls DamTailwater-September 2003 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL TOTAL NO. OF ORGANISMS 201 247 433 881 704 183 262 1149 2030 TOTAL NO. OF TAXA 12 16 20 27 27 23 17 34 42 EPT INDEX 2 2 3 5 3 4 2 4 6 NC BIOTIC INDEX 6.48 6.65 7.39 7.59 7.17 6.49 7.14 Pennington and Associates, Inc Page- 52 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam-September 2003 T1-75 T1-150' T1-225 T-1 T2-75 T2-150 T2-225 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Olinochaeta 7.2 5.1 FC 204 14 21 239 1 2 2 54 55 294 2 6.1 FC 11 28 96 135 4 3 65 72 207 *8 FC 5.7 FC 1 1 2 2 *8 FC 60 32 19 111 3 192 195 306 6.5 FC 6 6 6 *8 SC 6 6 6 SC 6.6 SC 13 13 1 5 6 19 8.8 CG 23 1 24 1 10 11 35 *6 SC 6.2 SC 8.2 SC 24 24 24 *1 CG Pennington and Associates, Inc Page- 53 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam-September 2003 T1-75 T1-150' T1-225 T-1 T2-75 T2-150 T2-225 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Tubificida Naididae *8 CG 4 15 19 19 Dero sp. 10.0 CG 1 2 3 3 Nais sp. 8.9 CG 1 1 3 3 4 Nais communis 8.8 CG Nais bretscheri *6 CG Pristina sp. 9.6 CG Pristina leidyi 9.6 CG Pristinella sp. 7.7 CG Ripistes parasita 2 FC Slavina appendiculata 7.1 CG Spirosperma sp. 5.3 CG Stylaria lacustris 9.4 CG 3 3 3 Tubificidae w.o.h.c. 7.1 CG 5 1 6 1 1 7 Limnodrilus hoffineisteri 9.5 CG Tubificidae w.h.c. 7.1 CG 1 5 6 6 Branchiura sowerbyi 8.3 CG Quistradrilus multisetosus 3.9 CG Lumbriculida Lumbriculidae 7.0 CG 2 6 8 1 1 9 Hirudinea *8 P 3 26 2 31 15 15 46 Erpobdellidae *8 P Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P Helobdella triserialis 9.2 P 1 1 1 Placobdella translucens 9.0 P ARTHROPODA Crustacea Ostracoda 1 2 2 5 5 Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. 1 1 1 Sidaidae Sida crystillina Copepoda Cyclopoida Pennington and Associates, Inc Page- 54 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam-September 2003 T1-75 T1-150' T1-225 T-1 T2-75 T2-150 T2-225 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 8 120 105 233 79 191 39 309 542 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG 1 1 1 Talitridae Hyalella azteca 7.8 CG 96 24 120 1 2 3 123 Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG 2 1 3 3 Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC 1 1 1 Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P 1 1 1 Corduliidae *5 P Neurocordulia sp. 5.0 1 1 1 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC 9 137 146 146 Hydroptilidae *4 PI Hydroptila sp. 6.2 PI 2 10 12 12 Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 93 13 106 2 2 121 125 231 Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P Pennington and Associates, Inc Page- 55 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam-September 2003 T1-75 T1-150' T1-225 T-1 T2-75 T2-150 T2-225 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Chironomidae Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 3 15 24 42 1 1 2 44 Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG 2 Cricotopus bicinctus 8.5 CG Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG 1 Dicrotendipes simpsoni 10.0 CG 1 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 2 12 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 1 17 Orthocladius (Euorthocladius) sp. *4 CG 1 Parachironomus sp. 9.4 CG Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudo chiron omus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC 21 17 Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 27 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH 2 3 19 133 155 157 1 5 6 6 1 2 2 2 4 1 3 51 54 55 1 1 1 5 19 5 5 24 1 19 4 3 2 9 28 1 4 20 24 25 16 16 16 1 1 1 5 5 5 38 2 39 636 677 715 1 1 1 27 27 1 1 2 2 2 2 2 Pennington and Associates, Inc Appendix 4.doc Page- 56 - 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam-September 2003 T1-75 T1-150' T1-225 T-1 T2-75 T2-150 T2-225 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL TOTAL NO. OF ORGANISMS 410 498 353 1261 110 284 1524 1918 3179 TOTAL NO. OF TAXA 18 26 22 37 19 16 21 31 48 EPTINDEX 3 2 3 1 3 3 3 5 NC BIOTIC INDEX 7.40 7.92 7.57 8.53 8.23 6.80 7.33 Pennington and Associates, Inc Page- 57 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-September 2003 T-1-210 T-1 420' T-1 630' T-1 T-2 250' T-2 500' T-2-750' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Oliqochaeta 7.2 5.1 FC 31 47 40 118 37 45 45 127 245 6.1 FC 1 1 3 9 5 17 18 *8 FC 5.7 FC *8 FC 535 516 999 2050 158 253 411 2461 6.5 FC *8 SC 5 5 27 15 2 44 49 SC 6.6 SC 16 5 120 141 8.8 CG 5 5 10 20 *6 SC 6.2 SC 8.2 SC 10 10 *1 CG 6 51 57 198 20 10 389 399 409 Pennington and Associates, Inc Page- 58 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-September 2003 T-1-210 T-1 420' T-1 630' T-1 T-2 250' T-2 500' T-2-750' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Tubificida Naididae Dero sp. Nais sp. Nais communis Nais bretscheri Pristina sp. Pristina leidyi Pristinella sp. Ripistes parasita Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w.h.c. Branchiura sowerbyi Quistradrilus multisetosus Lumbriculida Lumbriculidae Hirudinea Erpobdellidae Erpobdella punctata Rhynchobdellida Glossiphoniidae Batrachobdella phalera Helobdella sp. Helobdella stagnalis Helobdella triserialis Placobdella translucens ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida *8 CG 10.0 CG 1 1 30 6 36 37 8.9 CG 8.8 CG 10 10 1 20 21 31 *6 CG 9.6 CG 9.6 CG 7.7 CG 2 FC 7.1 CG 5.3 CG 1 1 1 9.4 CG 7.1 CG 90 90 5 3 8 98 9.5 CG 7.1 CG 8.3 CG 44 44 3 3 47 3.9 CG 7.0 CG 1 1 1 *8 P 6 1 7 1 5 6 13 *8 P P *8 P 3 3 6 6 7.6 P *6 P 9 9 9 8.6 P 6 6 17 33 16 66 72 9.2 P 1 1 1 1 2 9.0 P 5 5 5 15 15 15 5 5 5 5 5 5 Pennington and Associates, Inc Page- 59 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-September 2003 T-1-210 T-1 420' T-1 630' T-1 T-2 250' T-2 500' T-2-750' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 20 13 293 326 136 113 71 320 646 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG Talitridae Hyalella azteca 7.8 CG 5 2 7 10 5 15 22 Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG 10 1 11 11 Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI Hydroptila sp. 6.2 PI Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 2 10 12 10 5 5 20 32 Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P Pennington and Associates, Inc Page- 60 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-September 2003 T-1-210 T-1 420' T-1 630' T-1 T-2 250' T-2 500' T-2-750' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Chironomidae 10 25 35 35 Ablabesmyia mallochi 7.2 P 20 20 20 Ablabesmyia rhamphe gp. *6 P 5 5 7 5 12 17 Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG Cricotopus bicinctus 8.5 CG 5 5 5 Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG Dicrotendipes simpsoni 10.0 CG 454 127 323 904 133 195 347 675 1579 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 45 12 57 77 65 21 163 220 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 26 3 29 3 5 3 11 40 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 25 1 26 1 3 4 30 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH 5 5 5 Procladius sp. 9.1 P Pseudochironomus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC 10 10 10 Stenochironomus sp. 6.5 SH 5 5 5 Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 1 20 21 21 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH Pennington and Associates, Inc Page- 61 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-September 2003 T-1-210 T-1 420' T-1 630' T-1 T-2 250' T-2 500' T-2-750' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL TOTAL NO. OF ORGANISMS 1233 767 1960 3960 624 854 1007 2485 6445 TOTAL NO. OF TAXA 18 19 12 30 14 25 21 29 38 EPT INDEX 1 1 1 1 2 2 2 2 NC BIOTIC INDEX 8.75 8.27 8.33 8.62 8.65 8.76 8.54 Pennington and Associates, Inc Page- 62 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G High Rock-September 2003 T-1 T-1- T-1 T-1 T-2- T-2- T-2- T-2 TOTAL 110' 220 330' 160' 330' 490' 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Oligochaeta Tubificida Naididae Dero sp. Nais sp. Nais communis Nais bretscheri Pristina sp. Pristina leidyi Pristinella sp. Ripistes parasita Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w.h.c. Branchiura sowerbyi Quistradrilus multisetosus Lumbriculida 7.2 75 113 50 238 11 136 300 447 685 5.1 FC 6.1 FC 3 3 3 *8 FC 5.7 FC *8 FC 266 149 1592 2007 475 372 1067 1914 3921 6.5 FC *8 SC 1 SC 6.6 SC 8.8 CG *6 SC 6.2 SC 8.2 SC 35 *1 CG *8 CG 10.0 CG 8.9 CG 8.8 CG 10 *6 CG 9.6 CG 9.6 CG 7.7 CG 2 FC 7.1 CG 5.3 CG 9.4 CG 7.1 CG 9.5 CG 7.1 CG 8.3 CG 3.9 CG 1 1 1 7 35 20 3 3 3 13 10 1 1 10 10 20 1 1 2 2 2 10 17 17 4 27 62 3 3 11 24 1 1 20 30 Pennington and Associates, Inc Page- 63 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G High Rock-September 2003 T-1 T-1- T-1 T-1 T-2- T-2- T-2- T-2 TOTAL 110' 220 330' 160' 330' 490' 25% 50% 75% TOTAL 25% 50% 75% TOTAL Lumbriculidae 7.0 CG Hirudinea *8 P 2 2 2 Erpobdellidae *8 P 1 1 1 Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P 10 10 10 10 20 Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P 39 39 2 2 41 Helobdella triserialis 9.2 P 10 10 1 6 10 17 27 Placobdella translucens 9.0 P ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 226 214 122 562 328 127 113 568 1130 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG 6 1 7 2 3 5 12 Talitridae Hyalella azteca 7.8 CG Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC 1 1 1 Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI Hydroptila sp. 6.2 PI Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 5 5 2 2 7 Neureclipsis sp. 4.2 FC Pennington and Associates, Inc Page- 64 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G High Rock-September 2003 T-1 T-1- T-1 T-1 T-2- T-2- T-2- T-2 TOTAL 110' 220 330' 160' 330' 490' 25% 50% 75% TOTAL 25% 50% 75% TO TAL Diptera Chaboridae Chaoborus punctipennis 8.5 P Chironomidae 40 22 62 62 Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 2 2 2 Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG Cricotopus bicinctus 8.5 CG Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG Dicrotendipes simpsoni 10.0 CG 420 232 354 1006 452 201 340 993 1999 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 55 20 61 136 110 250 80 440 576 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 45 40 85 121 25 40 186 271 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 15 17 32 20 7 27 59 Phaenopsectra sp. 6.5 SC 3 3 3 Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudo chiron omus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC 10 3 13 13 Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 3 3 2 2 5 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 1219 837 2222 4278 1577 1151 1975 4703 8981 TOTAL NO. OF TAXA 15 13 9 21 16 19 11 25 29 EPTINDEX 1 1 2 1 1 2 NC BIOTIC INDEX 8.90 8.75 8.38 8.80 8.60 8.32 8.57 Pennington and Associates, Inc Page- 65 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam November 2003 T-1 250' T-1 500' T-1 750' T-1 T-2 150' T-2-300' T-2 450' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Al:n i.rh ?e4? 7.2 5 8 12 25 45 5 1 1 5.1 FC 1 1 50 75 1 1 6.1 FC 3 59 126 188 85 174 235 494 682 *8 FC 5.7 FC 4 4 27 35 9 9 44 *8 FC 2 23 29 54 35 35 89 6.5 FC *8 SC 3 SC 6.6 SC 2 3 8.8 CG 10 *6 SC 6.2 SC 8.2 SC *1 CG 3 1 5 9 3 13 3 1 1 4 9 10 28 33 2 9 14 27 1 2 2 Pennington and Associates, Inc Page- 66 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam November 2003 T-1 250' T-1 500' T-1 750' T-1 T-2 150' T-2-300' T-2 450' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Tubificida Naididae *8 CG Dero sp. 10.0 CG Nais sp. 8.9 CG Nais communis 8.8 CG Nais bretscheri *6 CG Pristina sp. 9.6 CG Pristina leidyi 9.6 CG Pristinella sp. 7.7 CG Ripistes parasita 2 FC Slavina appendiculata 7.1 CG Spirosperma sp. 5.3 CG Stylaria lacustris 9.4 CG Tubificidae w.o.h.c. 7.1 CG Limnodrilus hoffineisteri 9.5 CG Tubificidae w.h.c. 7.1 CG Branchiura sowerbyi 8.3 CG Quistradrilus multisetosus 3.9 CG Lumbriculida Lumbriculidae 7.0 CG Hirudinea *8 P Erpobdellidae *8 P Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P 1 Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P Helobdella triserialis 9.2 P Placobdella translucens 9.0 P ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida 1 5 6 4 4 10 11 11 11 1 4 5 27 1 28 33 2 2 2 1 1 1 3 4 4 3 1 4 4 1 1 1 Pennington and Associates, Inc Appendix 4.doc Page- 67 - 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam November 2003 T-1 250' T-1 500' T-1 750' T-1 T-2 150' T-2-300' T-2 450' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 9 37 2 48 20 17 12 49 97 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG 1 1 1 Talitridae Hyalella azteca 7.8 CG 1 120 27 148 2 8 10 158 Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC 8 8 1 1 9 Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P 1 1 1 Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC 1 1 1 Hydroptilidae *4 PI Hydroptila sp. 6.2 PI 1 1 8 8 9 Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 9 33 4 46 7 2 9 55 Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P Pennington and Associates, Inc Page- 68 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam November 2003 T-1 250' T-1 500' T-1 750' T-1 T-2 150' T-2-300' T-2 450' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Chironomidae Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG 1 Cricotopus bicinctus 8.5 CG Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG Dicrotendipes simpsoni 10.0 CG 2 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudo chiron omus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH 1 2 33 6 11 50 52 4 1 5 5 1 3 3 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Pennington and Associates, Inc Appendix 4.doc Page- 69 - 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam November 2003 T-1 250' T-1 500' T-1 750' T-1 T-2 150' T-2-300' T-2 450' T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL TOTAL NO. OF ORGANISMS 49 310 242 601 291 223 305 819 1420 TOTAL NO. OF TAXA 12 20 13 24 18 10 14 25 34 EPTINDEX 1 2 2 3 2 2 1 4 4 NC BIOTIC INDEX 7.99 7.66 6.73 7.09 6.52 6.46 6.94 Pennington and Associates, Inc Page- 70 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows-November 2003 T-1-75 T1-150' T-1-225 T-1 T-226% T-2-50% T2-75% T-2 TOTAL TOTAL TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Oligochaeta 1 7.2 4 22 4 30 9 5.1 FC 1 1 7 4 20 50 6.1 FC 104 80 334 518 32 2 28 62 580 *8 FC 5 5 5 5.7 FC 1 4 5 5 *8 FC 18 38 56 16 16 72 6.5 FC 1 1 1 *8 SC 1 1 9 9 10 SC 6.6 SC 2 4 6 3 8.8 CG 3 5 2 10 2 *6 SC 6.2 SC 8.2 SC 1 1 *1 CG 5 8 14 2 12 1 Pennington and Associates, Inc Page- 71 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows-November 2003 T-1-75 T1-150' T-1-225 T-1 T-226% T-2-50% T2-75% T-2 TOTAL TOTAL TOTAL Tubificida Naididae Dero sp. Nais sp. Nais communis Nais bretscheri Pristina sp. Pristina leidyi Pristinella sp. Ripistes parasita Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w.h.c. Branchiura sowerbyi Quistradrilus multisetosus Lumbriculida Lumbriculidae Hirudinea Erpobdellidae Erpobdella punctata Rhynchobdellida Glossiphoniidae Batrachobdella phalera Helobdella sp. Helobdella stagnalis Helobdella triserialis Placobdella translucens ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida *8 CG 10.0 CG 8.9 CG 8.8 CG *6 CG 9.6 CG 9.6 CG 7.7 CG 2 FC 7.1 CG 5.3 CG 9.4 CG 7.1 CG 14 9.5 CG 7.1 CG 8.3 CG 3.9 CG 7.0 CG *8 P *8 P P *8 P 7.6 P *6 P 8.6 P 9.2 P 9.0 P 2 16 6 6 22 10 10 3 2 2 7 17 8 8 8 5 5 5 Pennington and Associates, Inc Page- 72 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows-November 2003 T-1-75 T1-150' T-1-225 T-1 T-226% T-2-50% T2-75% T-2 TOTAL TOTAL TOTAL Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 9 15 43 67 53 55 63 171 238 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG Talitridae Hyalella azteca 7.8 CG Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI Hydroptila sp. 6.2 PI Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P 1 1 1 76 17 93 93 1 1 1 1 1 1 1 1 1 3 10 13 13 Pennington and Associates, Inc Appendix 4.doc Page- 73 - 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows-November 2003 T-1-75 T1-150' T-1-225 T-1 T-226% T-2-50% T2-75% T-2 TOTAL TOTAL TOTAL Chironomidae Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG 4 1 Cricotopus bicinctus 8.5 CG Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG 1 Dicrotendipes simpsoni 10.0 CG 8 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 3 4 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 1 Orthocladius (Euorthocladius) SID- *4 CG Parachironomus sp. 9.4 CG Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudo chiron omus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 2 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH 5 8 39 64 111 116 1 8 12 1 1 7 5 2 3 3 1 1 2 1 12 3 1 1 1 13 21 1 4 9 16 3 6 1 1 1 1 2 15 15 2 1 1 Pennington and Associates, Inc Appendix 4.doc Page- 74 - 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows-November 2003 T-1-75 T1-150' T-1-225 T-1 T-226% T-2-50% T2-75% T-2 TOTAL TOTAL TOTAL TOTAL NO. OF ORGANISMS 173 251 447 871 175 110 177 462 1333 TOTAL NO. OF TAXA 13 13 18 27 16 8 10 20 33 EPTINDEX 2 2 3 3 NC BIOTIC INDEX 6.91 7.30 6.63 7.69 8.02 7.68 7.19 Pennington and Associates, Inc Page- 75 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. High Rock-November 2003 T1- T2- T2- T1-25% 50% T1-75% T-1 T2-25% 50% 75% T-2 TOTAL TOTAL TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina 7.2 136 36 70 242 25 60 390 475 717 NEMATODA 2 2 2 MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata 5.1 FC Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea 6.1 FC 18 18 10 10 28 Sphaeriidae *8 FC Eupera cubensis 5.7 FC Musculium transversum *8 FC 105 48 325 478 169 124 839 1132 1610 Pisidium sp. 6.5 FC Gastropoda Mesogastropoda Hydrobiidae *8 SC Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae SC Ferrissia rivularis 6.6 SC 1 5 2 8 8 Physidae Physella sp. 8.8 CG 4 2 13 19 3 21 24 43 Planorbidae *6 SC Helisoma anceps 6.2 SC Pennington and Associates, Inc Page- 76 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. High Rock-November 2003 T1- T2- T2- T1-25% 50% T1-75% T-1 T2-25% 50% 75% T-2 TOTAL TOTAL TOTAL Menetus dilatatus 8.2 SC 18 1 8 27 36 30 30 96 123 ANNELIDA Oligochaeta *1 CG Tubificida Naididae *8 CG Dero sp. 10.0 CG 1 1 1 Nais sp. 8.9 CG Nais communis 8.8 CG 19 4 23 5 23 28 51 Nais bretscheri *6 CG Pristina sp. 9.6 CG 1 1 1 Pristina leidyi 9.6 CG Pristinella sp. 7.7 CG Ripistes parasita 2 FC 80 7 87 87 Slavina appendiculata 7.1 CG 3 3 3 Spirosperma sp. 5.3 CG Stylaria lacustris 9.4 CG 15 1 16 16 Tubificidae w.o.h.c. 7.1 CG 3 3 3 Limnodrilus hoffineisteri 9.5 CG Tubificidae w.h.c. 7.1 CG 1 1 1 Branchiura sowerbyi 8.3 CG Quistradrilus multisetosus 3.9 CG Lumbriculida Lumbriculidae 7.0 CG 1 1 1 Hirudinea *8 P 1 1 2 2 Erpobdellidae *8 P 5 5 5 Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P Batrachobdella phalera 7.6 P Helobdella sp. *6 P 5 5 5 Helobdella stagnalis 8.6 P 1 1 3 3 4 Helobdella triserialis 9.2 P 10 4 14 10 10 24 Placobdella translucens 9.0 P 2 2 2 ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Pennington and Associates, Inc Page- 77 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. High Rock-November 2003 T1- T2- T2- T1-25% 50% T1-75% T-1 T2-25% 50% 75% T-2 TOTAL TOTAL TOTAL Daphnia sp. Sidaidae Sida crystillina 1 1 5 3 8 9 Copepoda Cyclopoida Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 48 353 250 651 325 154 511 990 1641 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG 1 1 1 1 2 Talitridae Hyalella azteca 7.8 CG 1 1 1 Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG 1 Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI Hydroptila sp. 6.2 PI 11 Leptoceridae CG 2 2 5 20 3 30 53 58 1 1 1 1 1 1 11 11 Pennington and Associates, Inc Page- 78 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. High Rock-November 2003 T1- T2- T2- T1-25% 50% T1-75% T-1 T2-25% 50% 75% T-2 TOTAL TOTAL TOTAL Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 2 1 3 25 10 35 38 Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P Chironomidae 3 3 3 Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 4 4 4 Chironomus sp. 9.6 CG 5 10 15 15 Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG Cricotopus bicinctus 8.5 CG 6 6 3 3 9 Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG 10 10 10 Dicrotendipes simpsoni 10.0 CG 17 127 185 329 130 140 70 340 669 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 32 31 18 81 135 20 40 195 276 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 4 38 13 55 5 27 20 52 107 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 5 13 18 18 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudochironomus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC 22 8 30 47 47 77 Stenochironomus sp. 6.5 SH Pennington and Associates, Inc Page- 79 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. High Rock-November 2003 T1- T2- T2- T1-25% 50% T1-75% T-1 T2-25% 50% 75% T-2 TOTAL TOTAL TOTAL Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 5 5 3 3 8 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 472 673 902 2047 1010 677 1961 3648 5695 TOTAL NO. OF TAXA 25 20 16 35 19 20 10 26 42 EPTINDEX 3 3 2 5 2 1 2 2 5 NC BIOTIC INDEX 7.89 8.90 8.66 8.25 8.47 8.22 8.38 Pennington and Associates, Inc Page- 80 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-November 2003 T1-25% T1-50% T1-75% T-1 T2-25% T2-50% T2-75% T-2 TOTAL TOTAL TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina 7.2 50 420 470 61 26 51 138 608 NEMATODA 10 10 10 MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata 5.1 FC 5 5 5 Utterbackia imbecillis 1 1 1 Veneroida Corbiculidae Corbicula fluminea 6.1 FC 40 20 60 66 45 34 145 205 Sphaeriidae *8 FC Eupera cubensis 5.7 FC Musculium transversum *8 FC 1374 2483 550 4407 320 62 450 832 5239 Pisidium sp. 6.5 FC Gastropoda Mesogastropoda Hydrobiidae *8 SC Amnicola limosa 10 20 30 30 Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae SC Ferrissia rivularis 6.6 SC 9 17 26 26 Physidae Physella sp. 8.8 CG 20 10 10 40 2 2 42 Planorbidae *6 SC Helisoma anceps 6.2 SC Menetus dilatatus 8.2 SC 10 10 1 1 11 Pennington and Associates, Inc Page - 81 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-November 2003 T1-25% T1-50% T1-75% T-1 T2-25% T2-50% T2-75% T-2 TOTAL TOTAL TOTAL ANNELIDA Oligochaeta *1 CG Tubificida Naididae *8 CG Dero sp. 10.0 CG 40 40 Nais sp. 8.9 CG Nais communis 8.8 CG 40 Nais bretscheri *6 CG Pristina sp. 9.6 CG Pristina leidyi 9.6 CG Pristinella sp. 7.7 CG Ripistes parasita 2 FC 10 80 Slavina appendiculata 7.1 CG Spirosperma sp. 5.3 CG Stylaria lacustris 9.4 CG Tubificidae w.o.h.c. 7.1 CG Limnodrilus hoffineisteri 9.5 CG Tubificidae w.h.c. 7.1 CG Branchiura sowerbyi 8.3 CG Quistradrilus multisetosus 3.9 CG Lumbriculida Lumbriculidae 7.0 CG Hirudinea *8 P 11 Erpobdellidae *8 P 1 Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P 31 Helobdella triserialis 9.2 P 20 1 Placobdella translucens 9.0 P ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae 80 80 40 2 1 3 43 90 90 5 2 7 7 11 46 24 70 81 10 11 3 3 14 2 2 2 4 4 4 1 1 1 70 70 70 31 22 22 53 10 31 9 9 40 Pennington and Associates, Inc Appendix 4.doc Page- 82 - 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-November 2003 T1-25% T1-50% T1-75% T-1 T2-25% T2-50% T2-75% T-2 TOTAL TOTAL TOTAL Sida crystillina 20 20 20 Copepoda Cyclopoida 10 10 10 Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 570 152 410 1132 59 119 46 224 1356 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG Talitridae Hyalella azteca 7.8 CG Decapoda Cambaridae 7.5 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI Hydroptila sp. 6.2 PI Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 11 143 94 248 21 18 8 47 295 1 1 3 3 4 10 20 30 10 3 4 17 47 Pennington and Associates, Inc Page- 83 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-November 2003 T1-25% T1-50% T1-75% T-1 T2-25% T2-50% T2-75% T-2 TOTAL TOTAL TOTAL Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P 5 3 8 8 Chironomidae Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 5 5 5 Chironomus sp. 9.6 CG 10 10 4 4 14 Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG Cricotopus bicinctus 8.5 CG 2 2 2 Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG Dicrotendipes neomodestus 8.1 CG Dicrotendipes simpsoni 10.0 CG 70 60 210 340 15 16 22 53 393 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH 1 1 1 Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 90 40 220 350 373 145 44 562 912 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 10 10 1 1 2 12 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 42 51 10 103 5 2 7 110 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P 2 2 2 Pseudochironomus sp. 5.4 CG 3 3 3 Rheotanytarsus sp. 5.9 FC Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Pennington and Associates, Inc Page- 84 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Tuckertown-November 2003 T1-25% T1-50% T1-75% T-1 T2-25% T2-50% T2-75% T-2 TOTAL TOTAL TOTAL Xenochironomus xenolabis 7.1 P Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 2370 3221 2054 7645 997 504 710 2211 9856 TOTAL NO. OF TAXA 18 18 13 26 15 23 17 32 39 EPT INDEX 1 1 1 2 1 2 1 2 2 NC BIOTIC INDEX 8.38 7.97 8.30 8.30 8.44 8.02 8.20 Pennington and Associates, Inc Page- 85 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls D am June 2004 T-1 250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii 2 2 2 Dugesia tigrina 7.2 3 20 23 105 35 350 490 513 NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata 5.1 FC Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea 6.1 FC 12 96 476 584 58 134 342 534 1118 Sphaeriidae *8 FC Eupera cubensis 5.7 FC 11 6 40 57 1 1 58 Musculium transversum *8 FC 29 45 100 174 40 6 403 449 623 Pisidium sp. 6.5 FC Gastropoda Mesogastropoda Hydrobiidae *8 SC Amnicola limosa 4 4 1 1 5 Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae SC Ferrissia rivularis 6.6 SC 4 4 5 5 9 Physidae Physella sp. 8.8 CG 72 91 163 87 16 573 676 839 Planorbidae *6 SC Helisoma anceps 6.2 SC 5 10 15 15 Menetus dilatatus 8.2 SC 35 110 145 2 80 82 227 Pennington and Associates, Inc Page- 86 - Appendix 4.doc 10/22/ 2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam June 2004 T-1 250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL ANNELIDA Oligochaeta *1 CG Tubificida Naididae *8 CG 10 10 122 4 58 184 194 Dero sp. 10.0 CG Nais sp. 8.9 CG Nais communis 8.8 CG 3 3 61 12 73 76 Nais bretscheri *6 CG 4 4 4 Pristina sp. 9.6 CG Pristina leidyi 9.6 CG Pristinella sp. 7.7 CG Ripistes parasita 2 FC Slavina appendiculata 7.1 CG 13 30 90 133 31 29 60 193 Spirosperma sp. 5.3 CG Stylaria lacustris 9.4 CG 40 40 116 116 156 Tubificidae w.o.h.c. 7.1 CG 92 174 266 266 Limnodrilus hoffineisteri 9.5 CG 116 116 116 Tubificidae w.h.c. 7.1 CG Branchiura sowerbyi 8.3 CG Quistradrilus multisetosus 3.9 CG Lumbriculida Lumbriculidae 7.0 CG 7 40 30 77 306 56 87 449 526 Hirudinea *8 P 3 10 13 13 Erpobdellidae *8 P 5 5 1 1 6 Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P Helobdella triserialis 9.2 P Placobdella translucens 9.0 P ARTHROPODA Crustacea Ostracoda 1 1 1 Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Pennington and Associates, Inc Page- 87 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam June 2004 T-1 250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Sidaidae Sida crystillina 3 5 8 1 1 9 Copepoda 1 1 1 Cyclopoida Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 374 261 80 715 347 469 231 1047 1762 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG Talitridae Hyalella azteca 7.8 CG 14 511 45 570 15 17 224 256 826 Decapoda Cambaridae 7.5 1 1 1 1 2 Insecta Collembola Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG 10 10 10 Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae 1 1 1 Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI 1 1 1 Hydroptila sp. 6.2 PI 9 10 19 10 1 11 30 Leptoceridae CG Ceraclea sp. 2 CG 1 1 1 Pennington and Associates, Inc Page- 88 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls D am June 2004 T-1 250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Polycentropodidae *6 FC Cyrnellus fraternus *8 FC 1 5 21 27 27 Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P Chironomidae 19 30 60 109 45 27 30 102 211 Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe 9p. *6 P 33 80 310 423 5 10 15 438 Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG 2 2 200 124 50 374 376 Cricotopus bicinctus 8.5 CG 1 10 11 300 39 110 449 460 Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG 18 5 23 6 6 29 Dicrotendipes neomodestus 8.1 CG 10 180 190 6 20 26 216 Dicrotendipes simpsoni 10.0 CG 5 1 6 6 Dicrotendipes sp. 8.1 CG 5 2 7 7 Endochironomus sp. SH Einfeldia natchitocheae 17 10 80 107 1 1 108 Eukiefferiella claripennis 9p. 5.6 CG Glyptotendipes sp. 9.1 FC 4 10 14 14 Microtendipes pedellus 9p. 5.5 CG 20 20 20 Nanocladius distinctus 7.1 CG 5 5 5 Orthocladius (Euorthocladius) sp. *4 CG 1 1 1 Parachironomus sp. 9.4 CG 42 20 50 112 5 6 10 21 133 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG 1 1 1 Polypedilum flavum 4.9 SH 2 2 2 Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudochironomus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC 1 10 140 151 5 1 20 26 177 Pennington and Associates, Inc Page- 89 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Falls Dam June 2004 T-1 250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Tribelos fuscicorne 6.3 CG 25 290 315 1 1 316 Tribelos sp. 6.3 CG 1 1 1 Tvetenia bavarica gp. 3.7 CG 1 1 1 Xenochironomus xenolabis 7.1 P 13 5 1 19 1 1 20 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 792 1215 2274 4281 1844 994 3053 5891 10172 TOTAL NO. OF TAXA 35 22 24 43 20 35 22 40 53 EPT INDEX 4 1 3 5 1 1 1 5 NC BIOTIC INDEX 8.54 7.77 7.13 8.09 7.93 8.08 7.96 8.08 8.09 Pennington and Associates, Inc Page- 90 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam June 2004 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus 7.2 5.1 FC 12 12 12 42 30 35 107 111 12 20 143 250 6.1 FC 115 42 187 344 647 78 53 778 1122 *8 FC 5.7 FC *8 FC 3 11 14 142 4 5 151 165 6.5 FC *8 SC 25 25 51 20 10 81 106 SC 6.6 SC 8.8 CG 43 21 5 69 242 71 22 335 404 *6 SC 6.2 SC 8.2 SC 25 25 102 21 15 138 163 Pennington and Associates, Inc Page- 91 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam June 2004 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL ANNELIDA Oligochaeta Tubificida Naididae Dero sp. Nais sp. Nais communis Nais bretscheri Pristina sp. Pristina leidyi Pristinella sp. Ripistes parasita Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w.h.c. Branchiura sowerbyi Quistradrilus multisetosus Lumbriculida Lumbriculidae Hirudinea Erpobdellidae Erpobdella punctata Rhynchobdellida Glossiphoniidae Batrachobdella phalera Helobdella sp. Helobdella stagnalis Helobdella triserialis Placobdella translucens ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae *1 CG *8 CG 9 10.0 CG 8.9 CG 8.8 CG *6 CG 9.6 CG 9.6 CG 7.7 CG 2 FC 7.1 CG 44 5.3 CG 9.4 CG 17 7.1 CG 61 9.5 CG 7.1 CG 8.3 CG 3.9 CG 7.0 CG 44 *8 P *8 P P *8 P 7.6 P *6 P 8.6 P 9.2 P 9.0 P 9 20 15 35 127 18 44 44 53 145 180 50 75 169 889 84 616 1589 1758 17 254 132 386 403 61 254 6 260 321 40 40 40 40 84 1016 6 91 1113 1197 20 20 6 6 26 20 20 20 Pennington and Associates, Inc Page- 92 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam June 2004 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Sida crystillina Copepoda Cyclopoida Isopoda Asellidae Caecidotea sp. Amphipoda Crangonyctidae Crangonyx sp. Talitridae Hyalella azteca Decapoda Cambaridae Insecta Collembola Ephemeroptera Caenidae Caenis sp. Heptageniidae Stenacron interpunctatum Stenonema sp. Tricorythidae Tricorythodes sp. Odonata Coenagrionidae Argia sp. Corduliidae Neurocordulia sp. Gomphidae Gomphus sp. Neuroptera Sisyridae Trichoptera Hydropsychidae Cheumatopsyche sp. Hydroptilidae Hydroptila sp. Leptoceridae Ceraclea sp. Polycentropodidae Cyrnellus fraternus *8 SH 9.1 CG 124 746 448 1318 60 7.9 CG 7.8 CG 7.5 *7 CG 7.4 CG *4 SC 6.9 SC *4 SC 5.1 CG *9 P 8.2 P *5 P 5.0 5.8 P *4 FC 6.2 FC *4 PI 6.2 PI CG 2 CG *6 FC *8 FC 1 728 43 772 41 5 5 475 99 634 1952 41 813 5 12 12 12 Pennington and Associates, Inc Page- 93 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam June 2004 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Neureclipsis sp. 4.2 FC Diptera 40 40 40 Chaboridae Chaoborus punctipennis 8.5 P Chironomidae 10 5 15 40 24 45 109 124 Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 140 75 215 10 10 225 Chironomus sp. 9.6 CG Clinotanypus sp. P Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG 350 95 445 624 216 521 1361 1806 Cricotopus bicinctus 8.5 CG 1 25 26 108 54 55 217 243 Cryptochironomus sp. 6.4 P Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG 1 40 41 41 Dicrotendipes neomodestus 8.1 CG 1 50 75 126 30 10 40 166 Dicrotendipes simpsoni 10.0 CG 25 25 6 5 11 36 Dicrotendipes sp. 8.1 CG Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 4 10 10 24 10 1 11 35 Microtendipes pedellus gp. 5.5 CG 5 5 5 Nanocladius distinctus 7.1 CG 22 22 22 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 31 70 25 126 21 12 6 39 165 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH 5 5 5 Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudo chiron omus sp. 5.4 CG 20 20 20 Rheotanytarsus sp. 5.9 FC 20 20 20 Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC 20 15 35 35 Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 3 3 10 5 15 18 Pennington and Associates, Inc Page- 94 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G. Narrows Dam June 2004 T-1250' T-1-500' T-1-750' T-1 T-2-150' T-2-300 T-2-450 T-2 TOTAL 25% 50% 75% TOTAL 25% 50% 75% TOTAL Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 601 2448 1233 4282 4819 1137 1770 7726 12008 TOTAL NO. OF TAXA 20 19 20 32 22 19 21 29 37 EPTINDEX 1 1 1 1 2 NC BIOTIC INDEX 7.71 8.00 7.92 8.06 7.32 8.14 7.44 7.59 7.75 Pennington and Associates, Inc Page- 95 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G Tuckertown June 2004 T-1 T-1- T-1- T-1 T-2- T-2- T-2- T-2 TOTAL 250' 500' 750' 150' 300 450 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina 7.2 4 2 11 17 4 5 3 12 29 NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata 5.1 FC Utterbackia imbecillis 1 1 1 Veneroida Corbiculidae Corbicula fluminea 6.1 FC 4 6 10 22 32 54 64 Sphaeriidae *8 FC Eupera cubensis 5.7 FC Musculium transversum *8 FC 56 89 37 182 59 43 65 167 349 Pisidium sp. 6.5 FC Gastropoda Mesogastropoda Hydrobiidae *8 SC Amnicola limosa 2 5 1 8 7 1 1 9 17 Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae SC Ferrissia rivularis 6.6 SC 1 1 1 1 2 Physidae Physella sp. 8.8 CG 3 6 23 32 1 2 3 35 Planorbidae *6 SC Helisoma anceps 6.2 SC 1 1 1 Menetus dilatatus 8.2 SC 2 17 19 1 6 7 26 ANNELIDA Oligochaeta *1 CG Tubificida Naididae *8 CG 3 3 1 2 2 5 8 Dero sp. 10.0 CG 22 3 230 255 4 8 4 16 271 Nais sp. 8.9 CG Nais communis 8.8 CG 1 1 1 1 2 Nais bretscheri *6 CG Pristina sp. 9.6 CG Pristina leidyi 9.6 CG 2 2 2 Pristinella sp. 7.7 CG Ripistes parasita 2 FC 2 2 2 Slavina appendiculata 7.1 CG 10 10 9 6 15 25 Spirosperma sp. 5.3 CG Stylaria lacustris 9.4 CG Tubificidae w.o.h.c. 7.1 CG 1 43 44 4 4 8 16 60 Limnodrilus hoffineisteri 9.5 CG 14 14 2 2 16 Tubificidae w.h.c. 7.1 CG 1 1 1 Branchiura sowerbyi 8.3 CG Pennington and Associates, Inc Page - 96 - Appendix 4.doc 10/22/ 2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G Tuckertown June 2004 T-1 T-1- T-1- T-1 T-2- T-2- T-2- T-2 TOTAL 250' 500' 750' 150' 300 450 25% 50% 75% TOTAL 25% 50% 75% TOTAL Quistradrilus multisetosus 3.9 CG 2 2 2 Lumbriculida Lumbriculidae 7.0 CG Hirudinea *8 P 27 27 27 Erpobdellidae *8 P 1 5 19 25 25 Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P 24 24 3 3 27 Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P 7 32 39 4 8 1 13 52 Helobdella triserialis 9.2 P 1 8 14 23 3 6 4 13 36 Placobdella translucens ARTHROPODA Crustacea Ostracoda Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida Isopoda Asellidae Caecidotea sp. Amphipoda Crangonyctidae Crangonyx sp. Talitridae Hyalella azteca Decapoda Cambaridae Insecta Collembola Ephemeroptera Caenidae Caenis sp. Heptageniidae Stenacron interpunctatum Stenonema sp. Tricorythidae Tricorythodes sp. Odonata Coenagrionidae Argia sp. Corduliidae Neurocordulia sp. Gomphidae Gomphus sp. Neuroptera Sisyridae Trichoptera Hydropsychidae Cheumatopsyche sp. Hydroptilidae Hydroptila sp. 9.0 P 1 1 1 1 2 1 1 1 *8 SH 9.1 CG 64 84 161 309 75 81 92 248 557 7.9 CG 7.8 CG 1 8 9 1 1 2 11 7.5 *7 CG 7.4 CG *4 SC 6.9 SC *4 SC 5.1 CG *9 P 8.2 P *5 P 5.0 5.8 P *4 FC 6.2 FC *4 PI 6.2 PI 1 1 1 Pennington and Associates, Inc Page- 97 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G Tuckertown June 2004 T-1 T-1- T-1- T-1 T-2- T-2- T-2- T-2 TOTAL 250' 500' 750' 150' 300 450 25% 50% 75% TOTAL 25% 50% 75% TOTAL Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P 5 5 3 2 5 10 Chironomidae 3 5 8 2 3 5 13 Ablabesmyia mallochi 7.2 P Ablabesmyia rhamphe gp. *6 P 1 1 1 Chironomus sp. 9.6 CG 2 2 2 Clinotanypus sp. P 1 1 1 Coelotanypus sp. 8 P Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG Cricotopus bicinctus 8.5 CG 9 9 9 Cryptochironomus sp. 6.4 P 1 1 1 Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG 5 11 1 17 17 Dicrotendipes neomodestus 8.1 CG Dicrotendipes simpsoni 10.0 CG 3 3 3 9 2 6 8 17 Dicrotendipes sp. 8.1 CG 3 3 3 Endochironomus sp. SH Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 20 21 7 48 10 27 12 49 97 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 1 2 3 3 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 1 1 1 3 3 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P 1 1 9 9 10 Pseudo chiron omus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC 2 20 14 36 1 1 1 3 39 Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 238 278 651 1167 188 268 255 711 1878 TOTAL NO. OF TAXA 21 19 25 34 17 26 24 35 44 EPTINDEX 1 1 1 NC BIOTIC INDEX 8.54 8.50 9.08 8.83 8.45 8.34 8.29 8.35 8.65 Pennington and Associates, Inc Page- 98 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G High Rock June 2004 T-1 T-1- T-1- T-1 T-2- T-2- T-2- T-2 TOTAL 250' 500' 750' 150' 300 450 25% 50% 75% TOTAL 25% 50% 75% TOTAL COELENTERATA Hydrozoa Hydroida Hydridae Hydra sp. PLATYHELMINTHES Turbellaria Tricladida Planariidae Cura foremanii Dugesia tigrina NEMATODA MOLLUSCA Bivalvia Unionoida Unionidae Elliptio complanata Utterbackia imbecillis Veneroida Corbiculidae Corbicula fluminea Sphaeriidae Eupera cubensis Musculium transversum Pisidium sp. Gastropoda Mesogastropoda Hydrobiidae Amnicola limosa Viviparidae Cipangopaludina chinensis Basommatophora Ancylidae Ferrissia rivularis Physidae Physella sp. Planorbidae Helisoma anceps Menetus dilatatus ANNELIDA Oligochaeta Tubificida Naididae Dero sp. Nais sp. Nais communis Nais bretscheri Pristina sp. Pristina leidyi Pristinella sp. Ripistes parasita Slavina appendiculata Spirosperma sp. Stylaria lacustris Tubificidae w.o.h.c. Limnodrilus hoffineisteri Tubificidae w.h.c. 1 1 1 7.2 2 18 30 50 32 18 50 100 150 1 1 1 5.1 FC 1 1 1 6.1 FC 29 35 35 99 8 2 10 20 119 *8 FC 5.7 FC *8 FC 190 208 409 807 145 125 329 599 1406 6.5 FC *8 SC 11 11 1 SC 6.6 SC 8.8 CG 4 2 30 36 *6 SC 6.2 SC 8.2 SC 19 4 10 33 *1 CG *8 CG 17 24 41 10.0 CG 6 1 8 15 8.9 CG 8.8 CG *6 CG 9.6 CG 9.6 CG 7.7 CG 2 FC 1 1 7.1 CG 58 19 221 298 5.3 CG 9.4 CG 7.1 CG 12 1 13 9.5 CG 7.1 CG 8 8 3 4 90 11 16 10 6 5 5 1 12 15 22 58 46 147 180 5 31 72 5 16 31 20 25 25 1 9 2 55 66 364 6 4 10 23 8 Pennington and Associates, Inc Page- 99 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G High Rock June 2004 T-1 T-1- T-1- T-1 T-2- T-2- T-2- T-2 TOTAL 250' 500' 750' 150' 300 450 25% 50% 75% TOTAL 25% 50% 75% TOTAL Branchiura sowerbyi 8.3 CG 30 5 35 22 22 57 Quistradrilus multisetosus 3.9 CG Lumbriculida Lumbriculidae 7.0 CG 3 5 8 8 Hirudinea *8 P 4 4 8 8 Erpobdellidae *8 P 6 6 6 Erpobdella punctata P Rhynchobdellida Glossiphoniidae *8 P 10 10 10 Batrachobdella phalera 7.6 P Helobdella sp. *6 P Helobdella stagnalis 8.6 P 24 32 10 66 12 1 70 83 149 Helobdella triserialis 9.2 P 27 15 42 2 71 73 115 Placobdella translucens 9.0 P ARTHROPODA Crustacea Ostracoda 1 1 1 Candoniidae Candona sp. Cladocera Daphnidae Daphnia sp. Sidaidae Sida crystillina Copepoda Cyclopoida Isopoda Asellidae *8 SH Caecidotea sp. 9.1 CG 24 57 125 206 36 20 10 66 272 Amphipoda Crangonyctidae Crangonyx sp. 7.9 CG Talitridae Hyalella azteca 7.8 CG 6 6 1 1 7 Decapoda Cambaridae 7.5 Insecta Collembola 1 1 1 Ephemeroptera Caenidae *7 CG Caenis sp. 7.4 CG Heptageniidae *4 SC Stenacron interpunctatum 6.9 SC Stenonema sp. *4 SC Tricorythidae Tricorythodes sp. 5.1 CG Odonata Coenagrionidae *9 P Argia sp. 8.2 P Corduliidae *5 P Neurocordulia sp. 5.0 Gomphidae Gomphus sp. 5.8 P Neuroptera Sisyridae Trichoptera Hydropsychidae *4 FC Cheumatopsyche sp. 6.2 FC Hydroptilidae *4 PI Pennington and Associates, Inc Page- 100 - Appendix 4.doc 10/22/2004 Table 4A. Benthic Macroinvertebrates Collected From Yadkin River, Fall 2003 (No./-2mz) SPECIES T.V. F.F.G High Rock June 2004 T-1 T-1- T-1- T-1 T-2- T-2- T-2- T-2 TOTAL 250' 500' 750' 150' 300 450 25% 50% 75% TOTAL 25% 50% 75% TOTAL Hydroptila sp. 6.2 PI Leptoceridae CG Ceraclea sp. 2 CG Polycentropodidae *6 FC Cyrnellus fraternus *8 FC Neureclipsis sp. 4.2 FC Diptera Chaboridae Chaoborus punctipennis 8.5 P 3 10 13 1 5 6 19 Chironomidae 15 10 25 11 7 6 24 49 Ablabesmyia mallochi 7.2 P 1 1 1 Ablabesmyia rhamphe gp. *6 P 4 3 7 6 1 7 14 Chironomus sp. 9.6 CG 1 3 1 5 9 5 14 19 Clinotanypus sp. P Coelotanypus sp. 8 P 28 5 33 2 1 3 36 Conchapelopia sp. 8.4 P Cricotopus sp. *7 CG 2 2 2 Cricotopus bicinctus 8.5 CG 7 7 7 Cryptochironomus sp. 6.4 P 1 1 1 1 2 Diamesa sp. 8.0 CG Dicrotendipes lucifer 8.0 CG 26 26 5 5 31 Dicrotendipes neomodestus 8.1 CG 2 1 3 3 Dicrotendipes simpsoni 10.0 CG 1 35 20 56 24 12 30 66 122 Dicrotendipes sp. 8.1 CG 8 8 8 Endochironomus sp. SH 1 1 1 Einfeldia natchitocheae Eukiefferiella claripennis gp. 5.6 CG Glyptotendipes sp. 9.1 FC 49 33 82 150 95 40 285 367 Microtendipes pedellus gp. 5.5 CG Nanocladius distinctus 7.1 CG 3 2 5 5 Orthocladius (Euorthocladius) sp. *4 CG Parachironomus sp. 9.4 CG 33 72 60 165 4 4 41 49 214 Phaenopsectra sp. 6.5 SC Parakiefferiella sp. 5.4 CG Polypedilum flavum 4.9 SH Polypedilum illinoense 9.0 SH Procladius sp. 9.1 P Pseudochironomus sp. 5.4 CG Rheotanytarsus sp. 5.9 FC Stenochironomus sp. 6.5 SH Tanytarsus sp. 9.2 FC 1 5 6 2 1 3 9 Tribelos fuscicorne 6.3 CG Tribelos sp. 6.3 CG Tvetenia bavarica gp. 3.7 CG Xenochironomus xenolabis 7.1 P 5 5 5 Empididae 7.6 P Hemerodromia sp. *6 P Simuliidae *6 FC Simulium sp. 6.0 FC Tipulidae Tipula sp. 7.3 SH TOTAL NO. OF ORGANISMS 562 579 1074 2215 606 356 823 1785 4000 TOTAL NO. OF TAXA 22 23 25 35 26 29 21 37 46 EPTINDEX NC BIOTIC INDEX 8.07 8.38 8.04 8.14 8.42 8.47 8.29 8.37 8.24 Pennington and Associates, Inc Page- 101 - Appendix 4.doc 10/22/2004