NC0088919_Toxicity Report (AT-3)_20140502

TEIRRAC. Juest
ENVIRONMENTAL CONSULTANTS, P.C.
May 2, 2014
VIA CERTIFIED MAIL ARTICLE NUMBER: 7010 2780 0002 4630 7597
RECEIVED
N.C.Dept. of ENR
MAY 0 6 2014
Winston-Salem
Regional Office
Environmental Sciences Section
Div. of Water Resources.
N.C. DENR-
1621.Mail Service Center
Raleigh, NC 27699-1621
Ret DWQ Form AT-3 Submittal
NPDES Permit N'o. NCO088919
Former Coliseum"Shell
Forsyth County
To.Whom It May Concern:
On behalf of Quality Oil, Company, LLC; Terraquest Environmental Consultants, P.C. hereby
submits the enclosed Effluent Aquatic Toxicity Report, Form/Phase II Chronic Ceriodaphnia
(DWQ. form AT3) with supporting laboratory reporting. Note that this.facility is operating
under a Special Order by Consent (SOC) that requires chronic',toxicity monitoring only to
achieve compliance. The tox test failure is not a violation.
Sincerely,
TERRAQUEST ENVIRONMENTAL CONSULTANTS; P.C.
Ryan D. Kerins
Project Manager, ORC (PG1 #986481)
Enclosed: AT-3 Form and Lab Data Package
cc: Quality Oil Company, LLC— BradleySnover, NCDWM-UST—Thomas Moore, NCDENR-DWQ— Ron Boone
100 E. Ruffin Street • Mebane, North' Carolina 27302
Telephone [phone] • Facsimile [phone]
www.terraquestpc.com

Effluent Aquatic Toxtdty Report Form/Phase If Chronic Carltdsphtxla
TerraQues -Former Coliseum Shell ��0088919p Cpr Forsyth County
F
L taty. . IngTest ESC Lab Sciences comments Lab ID: L692310-04, -07
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4,,,✓`8, 4 4X. 514 '16t15 .08;35
Treakmt
pH teat
pH Final
D.O. ltifti
D O3 Final
etiap• InitU
Lirfl3. final
10 0?
• 4 5 .1
4 5
cantrull
CoWat
c bnw
8 .1
B. 0
8.0
; 7.7
7.7
7.8
8.1
8.0
7.8
8.0
8-.0
7.7
8.1
8 .3
8.4
8 .6
, 8 . 5
8.4
8.5
8.6
8.4
8.4
8.3
7.9
24 . 5
24.7
25.9
24.7
24.6
24 .8
25.3
25.6
25.3
125.3
125.6
25.3
C3filar&ri*
3 4 5 6 7 8 9 10 It 12 rim
29.2
Yng
31131
31
1. 24
28
129
29
127
30
32
Pltlt
L
I L
I L I
L
L
' L
I L.
L
I L
I L
l tent'A 4Young
12.5 (-� @3e
Ef leant # 1tafrlg
2 5 =(L)1V'r(UD")e
Eluet
45
Eflue nt%
90
Final Corm Mortality 13 0
% Control 3rd am .10 0 0
Control Repro GV e.200
1
2
3
4
5
6
7
8
9
10
11
12 !!!nj
1 0 of 3.0 f j_LO of 10
34:28
18
27
24
31
26
29
32129.
2''8
slgai�carlt?� �]' yes
L
L
L
L
L
L
L
L
L
L
4 79
FinalI4lartsR Y81 Meant
45 0A
Qr'fxaGon
7
14
0
0
11
16
[22
3
1 G
2 2
11.1
Rbamdrie�Anawsts: 12 5
Ropra LOEG= 2 5 %' HOEG=�s!'a
y
MOUMMsteel's Many -one Rank
L
LL
LL
L
162,01
NoM1 t31st€lb? nb fyjetjt;shapi=-Wilk
7
g
antis 0. 91246 C ifts2l 0.919
Equal vartanees? no 1Jleodpartlett
gtgtV= 39 .7303 'C F]tICa 11.3449
than-�'ar°ameMc �4na�slsliiannAcabtei'
ME&DtStee1' a Many -One Rank Test
S " Eitlrreet ft Rerile Ss Crttirat
N
mossomeno®MeOMMMMUMBONO
12.5
95.5A
7.00
25
55.00
77.00
45
55.00
77.00
:z Z 4 0 tx ! u Id lu fk= .��.
Effluer°le #Yt, 0. 0 0 0 0 0 0 0 0' 0 0 ;. :.
D D D D D D D: D D D 100ii>isZilrz
*Should rw'e higImttosat canwntraft or
ATr Environmental Scient:e5 Section hWrstemcento tmentlh ©.Q.n5Amgfl
MAIL DIv_ of Water Resources txRFaac�oettaozcor�m�rr�raauc�atdEan
- - N.D. DENR
{i21-Mail SenkeOentDr DWQ form AT-3 revised 2/2013
Raleigh, N.D. 276991621
1 of 19

TerraQuest-Former Coliseum Shell
NPDES #: NCO088919
Tue 4/8/14
Initials pH Con' DO Time Analyst
Control
Dup. Control
12.6
Dup. 12.5
26
Dup, 25
45
Dup, 45
90 (PL)
Dup. 90 (PL)
100
Dup. 100
NOWMI9990
-:I
-
7.7
52.11
8.6
15:41:27
BE
7.8
58
8.4
15:43:02
BE
7.8
58.2
8.4
15:43:20
BE
:1;Jit3
'bt
I s'
MIN
Mer
y
BA:
74.9
8.2
15:44:54
BE
75.2
8,1
15:4p5:11
BE
,y8A
AM
"gal
Note: 100% Effluent data required throughout test
Thu 4/10114
initials pH Con. DO Time Analyst
Control
Duplicate
12.6
26
45
90 (PL)
100
g
;
IMMA1A
t.-
11 ds
eMW
7.7
89-11
8.5
14:13:08
BE
7,9
1001
8.4
14:14:21
BE
1
8
131.2
8
14:15:15
BE
y_< B
ku:g s7
� ::4x'��
1 N
z
Note: 100% Effluent data required throughout test
Sun 4113/14
Initials pH Con. DO The Analyst
Control
Duplicate
12.6
26
46
90 (PL)
100
�-
7.8
66.2.
BA
10:11:43
BE
_
7.9
74.3
8.4
10:12:38
BE
!`ti' N ;hB
' E
r
°' $ if3
dTIR
r
BE
Note:. MO% Effluent data required throughout test
Initials
Centro
12.E
211
41
90 (PL
101
Test Data: Start: April 8, 2014
Login #: 1_692310-04, 07
Thu 4/10114 Cedodaphnia dubia
Finals pH DO Time Analyst
Control
Dup. Control
12,6
Dup. 12.5
26
Dup, 25
46
Dup, 45
90 (FL)
Dup. 90 (PL)
100
Dup. 100
8
8.5
16:14:30
W M
8
8.5
16:15:21
WM
8
B.5
16:15:42
WM
&M
Won
8
83
16:16:40
WM
8
8.5
16:26:58
WM
MM
!�1ad�.��rd:
L.i'`•"�or�8�
;�6:�Zi.'�0
t:;luGr=s
Sun 4113114 Ceriodaphnia dubia
Finals pH DO Time Analyst
Control
Duplicate
12.5
26
45
90 (PL)
100
t
LIE- i�3f i�
-��r�t:�_4:b1
iC'�h� j
8
88..5
14:45:131
BE
8
8.6
14:45:56
BE
BEK
Mon 4/14114 Ceriodaphnia dubia
Finals pH DO Time Analyst
Control
Duplicate
12.6
25
46
90 (PL)
100
TIM
M
W UN&9
7.7
8
16:22:36
BBM
v8.3
7.3
16:23:35
BBM
;aL?iF8
efts
h'tB6 °
B$fz'
BBM
pH
I Ion
I DO
range.
Imean
I range
Imean I
range
mean
7.7-7.8
7.7
2.1.89.4
69
8.4-8.6
a.5
7.7-7.9
7.8
54.6-96.4
69
8.4-35
8.5
7.8.7.9
7.9
58-100
73
8.4-8.4
8.4
7.9.8
63.2.109
79
83-14
8.4
9-8.174.9-131.2
El
1177.3-136.7
94
8.8.2
8.1
8-8.1
8.1
99
8-8,6
8.2
Finals
Contro
12,t
21
4t
90 (PL',
IN
Cndodanhnia dubia
pH
I DO
range
mean
range
mean
7.1--a
7.9
7.9.8.5
8.9
7.7-3
7.9
8.2-8.5
8.4
7.9-8
8.0
8.3.8.6
8.5
7.9-8
7.9
8.4.8.6
8.5
8-8
8.0
8.5-a.s
8.5
8.1-0.1
8.1
8.5.8.6
:8,6
2 of 19

TerraQuest-Former Coliseum Shell
NPDI=S # NCO088919 Test Date: Start: April 8, 2014
ESC Lab M L692310-04,-07
Preliminary Hardness (analyzed prior
fn fcef ininaflnn uetnn 1 anhaf) Anaivstt Date: Time.
(Preliminary Hardness 10% DMW
Determination) Control
39 mg/L 578 1. 4/8/2014 09:41
Alkalinity (analyzed In
L# of Control 10% DMW Wet Lab via LACHAT)
L692249-02 Control7 30 mg/L
Control Water Carboy(s)Used:. sR ' "`'';1, , -' `-°'
'E �:aN PN
Alkalinity (analyzed in
Hardness (analyzed in
Effluent Wet Lab via Lachat)
Wet Lab -via LACHAT)
Tue 4/8/14
Total Res. Cl 2
Tue 418/14
Thu 4/10/14
Analyst: (initial)
Control (initial)
Highest
Concentration
(initial)
Analyst: (final)
Control (final)
Highest
Concentration
(final)
46
mg/L
45
mg/L
Analyst
0.01
mg/L
BE
0.00
mg,
WM
-692249-01
Control Water (Range & Mean) - Alkalinity
range:30-30 1 mean: 30
Effluent Alkalinity (mg/L)
range:46-49 mean: 48
Effluent Hardness (mg/L)
range:45-49 1 mean: 47
TPmnara4iirP CPr1ndanhn1a dubla l°C1 /maasuramanf fakan finm surrnaate cunl
a Cis t, . ,.
OR
- MOLN",
�so
, g'F &mtw.
OUR-
r
ZIFF
®p �
r T ,.E
{ ba l -y
Thermometer serial number: 87870092
Record of Feedina Times - Cerlodaahnia. dubia
Time
Analyst
Tue 4/a/14
Thermometer serial number: 87870092
Record of Feedina Times - Cerlodaahnia. dubia
Time
Analyst
Tue 4/a/14
Wed 4/9114
Thu 4110/14
Fri 4111/14
Sat 4112114 Sun 4113/14
Mon 4/14/14
15:38
10:41
14:05
9:35
8:50 10:11
14:57
BE
BE
WM
WM
KR BE
8E
TerraQuest-Former Coliseum Shell
3of19

L•a•IR s•c•I•�.rr•c•�•s
NOTATIONS USED BY ANALYSTS DURING TOXICITY EVALUATIONS
Cedodaphnia dubia (water flea)
# numbers on the Reproduction bench sheets (chronic) indicate the number of live young produced
if number is circled, this Indicates movement of daphnid has become impaired either by actual algal
growth on the organisms, or has become entrapped in substances found in the effluent sample, or has
been covered in stalked cilia
ME (molted embryo) often a stressed or poor condition female will abort all or some of a brood in response
to a toxin, insufficient nutrition, or just an inability to sustain a certain level of reproduction
P (pale) this is a noticeable reduction in coloration compared to that which is normal for -the Individual's age
SS (small size) this observation is made in comparison to other individuals of the same brood or age group
and generally represents a difference'of at least 2X size difference
(erratic swimming) this represents a locomotor behavior typified by unsustained swimming with the
ES daphnld periodically "resting" on the bottom of the test vessel; this condition is often observed prior to a
daphnid becoming totally immotile
I (immotility) this denotes a total lack of motility; daphnid is on the bottom of the test vessel and is confirmed
as living; daphnlds are frequently dead within a short time
LIT (lost in transfer) organism was lost during transfer process; stats are adjusted to represent this dilution as
having one less organism
N L (not loaded) organism was not loaded at test intiation; stats are adjusted to represent this dilution having
one less organism
NT (not transferred) organism was not present at the time of the next transfer; stats are adjusted to represent
this dilution having one less organism loaded at the initiation of testing
X (dead) dead daphnid is on bottom of test vessel and is confirmed dead by observation of no appendage
movement and no visible heartbeat
Accreditation Is only applicable to the test methods specified on each scope of accreditation held by ESC Lab Sciences. 5 of 19

Date(s) and Time(s) of Neonate Harvest From 17:04 on 4/7/20144 to 23'.44 on 4/7/2014
Neonates were Harvested from the Following Tray(s): 040714XA7 040714XA1PH6
0714XA1 04OT14XA1 U0714XA1 U0714XA1 04onUM DW14XA1 UMAIXA1 040714XA7 Template Name: Maximum duration of
Neonates were Harvested from the Following Cups: H2 H3 I H7 1 11 1 13 1 14 1 J1 I J5 I J7 Sigma test: 7 days + 2 hrs
M n%. MAW) Login t L692310-04,-07
.. . • .: • -• - -e - - •�•:. .
•
- . -'�.•.
Idenfification
of Replicate•'
. •
w
')`
�t --y�am
ENR
z �.-'� �
�''
k e���i":'��.
N'`'�ri�'�lF�'...�'�f-
8 �a
-r.0' E'�,_0
r
CiQ +
•, �'1 ,
P.�.'�-'I�1"�.il7�.a�,�`,
4.
.:,.,,,,
?iC�
(� �r
tl:���.a•''
'•`i f(Sai. �i'�i�
i " A8 •�
�:�.s.�1�� "
''o&I�'�`
4.. •
I.F,C3�e�r��,
r . a
«:�I�,-,?,,'.:�s
•-r7,t��
�r•:.I��::;1�1
r t `•Tfj
^`=*,;'
ut,aii.�.ta.,'T:.j
.
" '.�
, y.�.
�;y`..-;
r' a
��€
�.,.
Pam,.
`�lYi•11'1'1
\I►y'l 1k.liF:ll�itT��k1..IiWCv�AI<f,:NE-1
\�/ C`
ii\I'�M26"lllmm��
omm
ption of Sample Being Analyzed Below: 12.5 TerraQuest-Former Coliseum Shell
& Transfer Data Identification of Replicate
Thu 4110/14
14:23
WM
48 hrs
0
0
00
0
11 0
1 0
01
0
Fri 4111114
9:44
WM
72 hrs
.59
Sat 4112114
8:53
KR
96 hrs
Sun 4/13/14
10:31
BE
120 hrS
7' 11
' 1
41
4'
5111
6'
5' 13
4111
5' 13
6' 13
4' '
Mon 4114114
14:48
BE
144 hrs
SURVIVAL
113
ONLY
Tue 4116114
8:39
BE
168 hrs
16
114 Ill
7 11
13
Ill
Ill
13 113
Total # of Young Produced: 1
34 1 28 1 18 1 27 1
24 1 31 1 26
1 297 32
1 29
' X' = indicates dead daphnid; death is confirmed by observation (no appendage movement and no visible heartbeat) -
Analyst Note: Numbers that have been divided by vertical dashes (- --) indicate that two separate broods were produced.
Z
Test Acceptability Criteria
Survival > 8D%
> 15 neonates/female
X Yes
No
> 80% 3rd brood
Is repro CV <40%
NCO088919
# of Offspring # of Live Adults
at Renewal at Renewal
0 10 ~
10
10
145 10
0 10 % Reduction
133 10 from Control
Total Offspring at Total Young Reproduction
Renewal Produced Mean
278 278 0

escription of Sample. Analyzed Below..Q- e e -um Shell NCO088919
Identification of Replicate
r �.'y iSi`�l""Y ` �•1
J�
"'MAR'•
i y 'i. iiU7`i
'G 16�' �I
m-•rc--zY^*'- r1c*'
� i{i 1 1{! I,n,l
"it[iiKr^:'t
/��'-t�
C �.ls�.,
•»
l'{r! `
�!—G
n •Ilc�..._0
' €tip-�'�'-,4
""''
"£��.
•�u'.f"'a'`ti...
' ij�' ifP—"'+
i1���iJ�
wS"`
,...7{i.-1.. •"'�
Ix ��.:'r". lOrty{( f` s
_... F: ���
����l�+TlC,�
Z,A :° •ry,. �`�4'{���{{�
�r !•�7�-im•^_^
T'i
+� �'. +�-�
'S � �.'i #.
(t- kT.Y
�5 {��,F
2Y�k�r
�^./
p'u-tu.w.•La
lt: Y�{
''�. r
i. �G l'l�(
,'._-
.{irT���}•^K
I... �..�t �3a
5.t+nt�
�Vi-.
�k�Z��:�:t.�.�(;
����W...1.t47.�:.'�t.#..1s+�U�wadJ�iryi�u:...�J..:
ps'.��M'ht.'�..t i
�I...��i�Ykc':3&iG
' �5�, �}j �;..NJ
..it.�iSr::{"'"*'.'.�'v^.i.�iF;:�;
c i_iTr�
i
iI R
11WE�n1
IIC iilIM10
1I
iFM�yc_
®�Wi('i1�1=31�i1i�'ii
IPj--Ma
�ililiW"M
.�M{
0
NUM, T,,qm
mom, M1
MM
-
0M
E,iM���00
Total # of Young Produced
Description of Sample Being Analyzed Below- 45 TerraQuest Former Coliseum Shell NG0088919
Set-up &Transfer Data Identification of Replicate # of Offspring # of Live Adults
,r, i .}'{{r .y,;,, ._ _ _ .w a_r:i ai •'ti•}.: • ::- ti••• A• 4 R- i. r_• n• F- 1 F• r, R m- a i 3 j 7 at Renewal at Renewal
EMS®
c%�
o �� ;i
if �F111CIIffinI
II[ Ni_ITn1iNMIT_ral-
=1IFA
i O
0��
Total # of. Young
Produced:
W = indicates dead,daphnid; death is confirmed by observation (no appendage movement and no visible heartbeat)
Analyst Note: Numbers that have been divided by vertical dashes (---) indicate thattwo separate broods were produced.
M
—n
3
4
2
2
Dial Young
% Reduction
from Control
Reproduction
Mean
% Reduction
1
from Control
ReproduOon
Produced
Mean
2
99.5 - 70/0

�aa •n �u w
I
0
Sun 4113114
120 hrs
Mon4114/14
144 hrs
0
Tue 4116114
168 hrs
0
Total Offspring at
Renewal
Total Young
Produced
Total # of Young Produced: 0 0
0 0 0 0 0 0 0 0
0
0
Sun 4113/14
120 hrs
11`
-
0
Mon 4114114
144 hrs
Tue 4115114
1 168 hrs
`
Total Offspring at
Renewal
Total•Young
Produced
Total # of Young-Produced:1 0
1 0 1 0 0 0 0 0 0 0 0
0
0
"JN = indicates dead daphnid; death.is confirmed by observation (no appendage movement and no visible heartbeat)
Analyst Note: Numbers that have been divided by vertical dashes (---) indicate that two separate broods were produced.
CD
O
h
% Reduction
from Control
Reproduction
Mean
. b0
% Reduction
from Control
Reproduction
`Mean
l bo

Ceriodaphnia Survival and Reproduction Test-7 Day
Survival
Start bate:
4/8/2014 Test ID: NCO088919
Sample ID:
L692310-04; 07
End Date:
4/15/2014 Lab ID: ESC Lab Sciences
Sample Type:
EFF2-Industrial
Sample Date:
Protocol: EPAF 94-EPA Freshwater
Test Species:
CD-Ceriodaphnia dubia
Comments:
TerraQuest - Former Coliseum Shell
Conc% 1 2 3 4 5 6 7 :8 9 10
B-Control 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
12.5 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
25 1.0000 1.0000 0.0000 0.0000 1.0000 1.0000 1.0000 1.0000 1.0000 1.0000
45 0.0000 0.0000 0.0000 0.0000 1.0000 0.0000 0.0000 0.0000 0.0000 0:0000
90 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000
100 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000 0.0000' 0.0000 0.0000 0.0000
Not
Fisher's 1-Tailed
Conc-/o
Mean
N-Mean
Resp
Resp
Total
N
Exact P
Critical
B-Control
1.0000
1.0000
1.0472
0
10
10
10
12.5
1.0000
1.0000
1.0472
0
10
10
10
110000
0.0500
25
0.8000
0.8000
0.94248
2
8
10
10
0.2368
0.0500
*45
0.1000
0.1000
0.57596
9
1
10
10
0.0001
0.0500
90
0.0000
0.0000
0:5236
10
0
10
10
100
0.0000
0.0000
0.5236
10
0
10
10
Hypothesis Test (1-tall, 0.05) NOEC LOEC ChV TU
Fisher's Exact Test 25 45 33.541 4
Treatments vs B-Control
Dose -Response Plot
0.9
0.8
0.7
20.6
y 0.5
G 0.4
~ 0.3
0.2
0.1
pp N N � rn o
+C'
O r-
O
U
Ch
Page 1 ToxCalc v5.0.23 Reviewed byus
9of19

Start Date:
4/8/2014
Test ID:
NCO088919
5ampie u:
Loaasiu-ul+F vI
End Date:
4/15/2014
Lab ID:
ESC Lab Sciences
Sample Type:
EFF2-Industrial
Sample Date:
Protocol:
EPAF 94-EPA Freshwater
Test Species:
CD-Cedodaphnia
dubia
Comments:
TerraQuest - Former
Coliseum Shell
Conc =/6
1
2
3
4 5
6
7 8
9
10
B-Control
31.000
31.000
31.000
24,000 28.000
29.000
29.000 27.000
30.000
32.000
12.5
34.000
28.000
18.000
27.000 24.000
M.000
26.000 29.000
32.000
29.000
25
7.000
14.000
0.000
0.000 11.000
16.000
22.000 3.000
16.000
22.000
45
0.000
0.000
0.000
0.000 0.000
0.000
2.000 0.000
0.000
0.000
90
0.000
0.000
0.000
0.000 0.000
0.000
0.000 0.000
0.000
0.000
100
0.000
0.000
0.000
0.000 •0.000
0.000
0.000 0.000
0.000
0.000
Conc%
Mean
N-Mean
Mean
Min
max
UVI/O
B-Control
29.200
1.0000
29.200
24.000
32.000
8.200
12.5
27.800
0.9521
27.800.
18.000
34.000
16.247
*25
11.100
0.3801
11.100
0.000
22.000
74.949
*45
0.200
0.0068
0.200
0.000
2.000
316.228
90
0.000
0.0000
0.000
0.000
0.000
0.000
100
0.000
0.0000
0.000
0.000
0.000
0.000
10 0.640 2.137 4.675
10 8.273 2.137 4.675
10 13.254 2.137 4.675•
10
10
Auxiliary Tests
Statistic
Critical
Skew
Kurt
Shapiro-Wilk's Test indicates non -normal distribution (p <= 0.01)
0.91246
0.919
-0.3241,
1.44784
Bartlett's Test Indicates unequal variances (p =1 22E-08)
39.7303
11.3449
Hypothesis Test (1 tail, 0.05) NOEC LOEC ChV TU
MSDu MSDp
MSB MSE
F-Prob
df
Dunnett's Test 12.5 25' 17.6777 8
4.67497 0.1601
1941.69 23.9361
4.4E-1.6
3,36
Treatments vs B-Control
Dose -Response Plot
40
35
30
o 25
a 20
0
a15
10
5
4_........4 x.................................I
0 0
2N
N
CDO
C r
O
9
ca
1-tail, 0.05 level
of significance
Page 1 ToxCalc v5.0.23 Reviewed by:_J w I
10of19

/
Ceriodaphnia Survival and Reproduction Test -Reproduction
Start Date:
4/8/2014 Test ID: NCO088919
Sample ID:
L692310-04,-07
End Date:
4/15/2014 Lab ID: ESC Lab Sciences
Sample Type:
EFF2-Industrial
Sample Date:
Protocol: EPAF 94-EPA Freshwater
Test Species:
CD-Ceriodaphnia dubia
Comments:
TerraQuest - Former Coliseum Shell
C °
1 2 3 4 5 6
7 8
9 10
one -a
B-Control
31.000
31,000
31.000
24.000
28.000
29.000
29.000
27.000
30.000
32.000
12.5
34.000
28.000
18.000
27.000
24.000
31.000
26.000
29.000
32.000
29.000
25
7.000
14.000
0.000
0.000
11.000
16.000
22.000
3.000
16.000
22.000
45
0.000
0.000
0.000
0.000
0.000
0.000
2.000
0.000
0.000
0.000
90
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
100
0.000
0,000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
0.000
Transform: Untransformed
Conc%o
Mean
N-Mean
Mean
Min
Max
CV%
N
B-Control
29.200
1.0000
29.200
24M0
32.000
6.200
10
12.5
27.800
0.9521
27.800
18.000
34.000
16.247
10
*25
11.100
0.3801
11.100
0.000
22.000
74:949
10
*45
0.200
0.0068
0.200
0.000
2.000
316.228
10
90
0.000
0.0000
0.000
0.000
0.000
0.000
10
100
0.000
0.0000
0.000
0.000
0.000
0.000
10
7-Tailed
Critical
95.50 77.00
55.00 77.00
55,00 77.00
Auxiliary Tests Statistic Critical Skew Kurt
Shapiro-WilWs Test indicates non -normal distribution (p <= 0.01) 0.91246 0.919-0.3241 1.44784
Bartletfs Test Indicates usual variances (p =1.22E-08) 39.7303 11.3449
Treatments vs B-Control
Dose -Response Plot
40
35
30
o 25
:a
0
-520
0
a1s
10
5
0
CC� N
m
Ln o 0
1 CD0
Page 1 ToxCalc v5.0.23 Reviewed by:0''�
11 of19

Control Chart for April 2014 Acute C.dubla Reference Toxicant
CUu/ = 93 9
0.49
0.44
0.39
J
0.34
U
0
0.29
0
0.24
0.19
0.14
0.09
4''beN e, N"°°\^e"��,��°°\o
Date
+2 SD
+1 SD
Mean
-1 SD
-2 SD
Dates
Values
Mean
-1 SD
-2 SD
+1 SD
+2 SD
12/06/12
0.3536
01 /09/13
0.3318
0.3427
0.3273
0.3119
0.3581
0.3735
02/06/13
- 0.2102
0.2985
0.2213
0.1440
0.3758
' 0.4531
03/06/13
0.2588
0.2886.
0.2225
0.1563
0.3547
0.4209
04/03/13
0.3536
0.3016
0.2374
0.1731
0.3658
0.4301
05/02/13
0.2856.
0.2989
0.2411
.0.1833
0.3568
0.4146
06/05/13
0.1833
0.2824
0.2139
0.1453
0.3509
0,4195
07/10/13
0.1961
0.2716
0.2012
0.1308
0.3420
0.4124
08/08/13
0.1896
0.2625
0.1912
0.1199
0,3338
0.4051
09/04/13
0.1789
0.2541
061819
0.1096
0.3264
0,3986
10/02/13
0.2108
0.2502
0.1804
0.1106
0.3200
0,3898
10/09/13
0.2082
0.2467
'0.1791
0.1115
0.3143
0.3820
11/01113
0.2415
0.2463
0.1815
0.1168
0,3111
0,3758
12/04/13
0.2415
0.2460
0.1837
0.1215
0.3082
0.3704
01/03/14
0.2588
0.2468
0.1868
0,1267
0.3069
0.3669
02/05/14
0.35361
0.2535
0.1896
0.1257
0.3174
0.3812
03/05/14
0.3055
0.2565
0.1934
0.1303
0.3197
0.3828
04/02/14
0.3186
0.2600
0.1970
0.1341
0.3230
0.3859
04/02/14
0.3078
0.2625
0.2004
0..1382
0.3247
0.3868
04/02/141
0.30781
0.2648
0.2034
0.1421
0.3261
0.3875
12065 Lebanon Rd
OESCMt. Juliet, TN 37122
[phone]
4 , A , y i , , G , 815.758.5859 fax
April 2014
Reference Toxicant Test
12 of 19

Control Chart for April 2014 Chronic C.dubla Reference Toxicant
CV% =19.4
0.33
v
0.28
Y
c
0 0.23
v
c 0.18
d
a
U 0.13
0.08
o�\oo\�b\o oa\o\o�\0�\06�0\��\0�\0\0�\0 �o �\o�\�^\��\�^ ry�\,�� �6\���o o�\o��\o�^N\o��^�\�`,\QN\,�a
Date
+2 SD
+1 SD
Mean
-1 SD
-2 SD
Dates
Values
Mean
-1 SD
-2 SD
+1 SD
+2 SD
04/02/13
0.2190
05/14/13
0.2289
0.2240
0.2169
0.2099
0.2310
0.2380
06/04/13
0.1496
0.1992
0.1560
0.1127
0.2424
0.2856
07/09/13
0.2404
0,2095
0,1686
0.1277
0.2503
'0.2912
08/06/13
0.2486
0.2173
0.1778
0.1383
0.2568
0,2963
08/27/13
0.2320
0,2198
0.1839
0.1481
0.2566.
0.2914
08/27/13
0.1640
0,2118
0.1729
0.1340
0.2507
• 0.2896
09/05/13
0.1753
0.2072
0.1690
0.1307
0.2455
0.2837
09/10/13
0.2282
0.2096
0.1731
0.1366
0.2460
0.2825
10/01/13
0.2114
0.2097
0.1764
0.1410
0.2441
0.2785
11/05/13
0.0993
0.1997.
0.1631
0.1-065
0.2463
0.2929
11 /26/13
0.2117
0.2007
0.1561
0.1115
0.2453
0.2899
11/26/13
0.2461
0.2042
0.1597
0.1152
0.2487
0.2932
12/12/13
0.1887
0.2031
0.1601
0.1172
0.2460
0.2890
01/07/14
0.2281
0.2048
0.1629
0.1210
0.2466
0.2885
02/04/14
0.2474
0,2074
0.1656
0.1237
0.2493
0.2911
02/11/14
0.1654
0.2049
0.1632
0,1214
0.2467
0.2885
02/11/14
0.2498
0.2074
0.1655
0.1237
0.2493
0.2912
03/04/14
0.2502
0.2097
0.1678
0.1259
0.2516
0.2934
04/01/141
0.22701
0.2106
0.1696
-0.1287
0.2515
0.2924
12065 Lebanon Rd
OESCMt, Juliet, TN 37122
[phone]
L -A•'a S•C•t•w•N-0-E•5 6 15.758.5859 fax
April 2014
Reference Toxicant Test
13 of 19

Control Chart for April 2014 Acute Minnow Reference Toxicant
CV%= 10.3
1.7
1.5
o�
1.3
Y
0
N
1.1
0.9 /
+2 SD
+1 SD
Mean
-1 SD
-2 SD
0.7
Ib
Ib
�\' I.\,o �`'\o�'`o\o �16 ry9\ �°o\^��o`�^��Qo\^°�I:b\ �N\ �d\o��°o\ 'b\o`�°h\°o\o°�°�°°�°0 \,O��
Date
Dates
Values
Mean
-1 SD
-2 SD
+1 SD
+2 SD
06/02/13
1.3315
06/08/13
1.1061
1.2188
1.0594
0,9000
1.3782
1.5376
06/10/13
1.2423
1.2266
1.1131
0.9996
1.3401
1.4537
06/05/13
1.2861
1.2415
1.1442
1.0468
1.3388
1.4362
07/10/13
1.2423
1.2417
1.1574
1.0731
1.3260
1.4103
08/08/13
1.2861
1.2491
1.1715
1.0940
1.3266
1.4042
08/29/13
1.1196
1.2306
1.1445
1.0585
1.3166
1.4027
09104/1.3
1.2000
1.2268
1.1463
1.0659
1.3072
1.3876
10/02/13
1..0091
1.2026
1.0981
0.9936
1.3071
1.4116
10/09113
1.1965
1.2020
1.1034
1.0049
1.3005
1.3990
10109/13
:0.9352
1A777
1,0544
0.9311
1.3010
1.4244
11/01113
1:0815
1.1697
1.0489
0.9281
1.2905
1.4113
12/04/13
1.3199
1.1812
1.0583
0.9353
1.3042
1.4271
01/08/14
1.31.99
1.1912
1,0674
0.9435
1.3150
1.4388
01/23/14
1.0665
1.1828
1.0593
0.9357
1.3064
1.4300
-02/05114
1.0688
1,1757
1.0530
0.9302
1.2984
1.4212
03/05/14
1.0688
1.1694
1.0478
0.9262
1.2911
1.4127
04/02/14
. 1.1821
1.1701
1.0521
0.9340
1.2882
1.4062
04-102/14
1.2980
1..1769
1.0585
0.9400
' 1.2953
1.4137
04/02/14
1.0169
1.1689
1.0482
0.9275
1.2895
1A102
12065 Lebanon Rd
OESCMt. Juliet, TN 37122
[phone]•
L.-A, M S , C i , ty �. , 4 615.758.5859 fax
April 2014
Reference Toxicant Test
14of19

Control Chart for April 2014 Chronic Minnow Reference Toxicant
CV% = 8.16
0.6
0.5
�`� °N� N N o�,��ry,°��o��
Date
+2 SD
+1 SD
Mean
-1 SD
-2 SD
Dates
Values
Mean
-1 SD
-2 SD
+1 SD
+2 SD
11/06/12
0.9022
12/04/12
0.8640
0.8831
' 0,8561
0.8291
0.9101
0.9371
01/08/13
0,9312
0.8991
0.8654
0.8317
0.9328
0.9665
02/05/13
0.8960
0.8984
0.8708
0.8432
0.9259
0.9535
03/05/13
0.8500
0.8887
0.8565
0.8243
0.9209
0.9531
04/02/13
0.9246
0.8947
0.8623
0.8300
0.9270
0.9593
05/07/13
0.9375
0.9008
0.8671
0.8335
0.9344
0.9681
06/04/13
0.8545
0..8950
018598
0.8246
0.9302
0.9654
07/09/13
0.9375
0.8997
0.8639
0.8280
0.9356
0.9714
08/06/13
0:8517
- 0.8949
0.8579
0.8208
0.9320
0.9690
09/10/13
0.9176
0.8970
0.8612
0.8254
0.9328
0.9686
10/01/13
0.9276
0.8995
0.8643
0.8290
0.9348
0,9701
10/16/13
0,8616
0.8966
0.8613
0.8259
0.9320
0,9673
10/16/13
0.8714
0.8948
0.8602
0.8255
0.92S5
0.9641
11/05/13
0.9375
0.8977
0.8625
0.8274
0.9328
0.9680
12/03/13
0.9292
0.8996
0.8648
0.8299
0,9345
0.9694
01/07/14
0.6130
0.8828
0.8055
0.7282
0.9601
1.0373
02/04/14
0.9343
0.6856
0.8097
0.7337
0.9616
1.0375
03/04/14
0.9128
0.8871
0.8130
0.7389
0.9611
1.0352
04/01/14
0.9205
0.8887
0.8163
0.7438
0.9612
1.0337
12065 Lebanon Rd
VESCMt. Juliet, TN 37122
[phone]
L, A, , i , , ��� , C.. �, 615,758,5859 fax
April 2014
Reference Toxicant Test
15 of 19

Ur A?,7 t a
ANALYTICAL PURCHASE ORDER
Terraquest Environmental Consultants, P.C.
Client: Date:
Project Name: Project Manager:
Purchase Order No: Sampler:
PAP No ): TF:
Lab;
TF TASK
METHOD
MATRIX
PRICE/EA.
QTY
SUBTOTAL
A
acu
f•
33
G�
IS dv
.e—
a1�FCoucaakaF
MIT -NOD
(gi5%bb
��5� 00
Total:
Ii156,5a
Note -to Lab. Should the type of number of analysis differ for any reason, please notify
our office, [phone], immediately.
17 of 19

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