NC0006351_Additional Tox testing_20200302

TW,Jint.E
P.O. BOX-16414, Greenville, SC 29606
(E&) B77-6942 . FAX [phone]
4 Craftsman Court, Greer, SC 29654
Ceriodaphnia dubia Survival and Reproduction Test
EPA-821-R-02-013 Method 1002
Client: VENATOR CHEMICALS
Sample ID: #5 EFFLUENT
NPDES #: NC0006351
Test Date: 12-Feb-20
Laboratory Sample ID #: T55887
Test Reviewed and Approved By:
Robert W. Kelley, Ph.D.
QA/QC Officer
Afmak
ertiticatian #E87819
Test results presented in this report conform to all requirements of
NELAC, conducted under NELAC Certification Number E87819
Florida Dept. of Health. Included results pertain only to provided samples.
Farhad Rostampour
Laboratory Director
SCDHEC Certification #23104
NCDENR Certification # 022
Page 1 of 5

Effluent Aquatic Toxicity Report Form/Phase II Chronic Ceriodaphnia
Facility: VENATOR CHEMICALS #5 EFFLUENT NPDES # NC0006351 Pi e# 001 County: Cabarrus
Laboratoryy Performing Tes�12
r Comments:
SI nature of O.R.C. Signature of Lab Supervisor
Test Start Date Time End Date Time
Info 02/12/20 1:50 02/19/20 11:01
Sample Information Sample 1 Samplo 2 Control Start (Renewal 1 (Renewal 2 Start Renewal 1
Renewal 2
Control
Collection Start Date 02/11/20 02/13/20 Treatment 4% 4% 4% Control Control
7.7
Grab pH Init. 7.8 8.0 7.81 77 78
7.8
Composite (Duration) 24 hr 24 hr pH Final 8.1 8.1 79 8.2 8.1
8.0
Hardness (mg/L) 80.0 D O. Init 7.5 8.6 83 7.5 85
7.7
Spec. Cond. (umhos.cm) 4010 3930 302 D.O. Final 87 8.2 7.6 80 82
246
Chlorine (mg/L) 0.17 0.15 Temp Init 250 24.8 24.6 24.7 24.6 1
259
Sample temp. at Receipt 0.7 0.6 Tonp Final 25.3 25.0 2591 253 1 25.0
eSU t5
ronlc Test
Final Cont Mort %
0.0%
100%
Organism # % Conl3rd Brood
1 2 3 4 5 6 7 8 9 10 11 12 Mean contra Repro CV 1
6%
Control
# Young
23
23
23
20
20
22
21
24
24
22
none
none
22.2
48 Hour Mortality
(L)ive
(D)ead
L
L
L
L
L
L
L
L
L
L
none
none
Control WC
0of10 0of10
1 2 3 4 5 6 7 8 9 10 11 12 Moan
:Si nificant? No
Effluent %
0.24%
# Young
23
21
22
22
19
21
22
24
22
24
none
noneHO.9%
Final Mortality Sign. @
°/o or no CDn
Adult
(L)ive
Dead
L
L
L
L
L
L
L
L
L
L
none
none
Reproduction Analyses
1 2 3 4 5 6 7 8 9 10 Mean
(Repro LOEC= >3.84% NOEC= 3.84%
Method: Steel's Test
Effluent I°
# Younq
25
22
25
21
22
20
22
21
23
22.4
Adult
]23
% Red
(Normal Dist? no Method Kolm°gorov
(L)ive
(D)ead
L
L
L
L
L
L
L
L
L-0.9%
Statistic 0.971 Critical 0.895
(Equal Var? NA Method
1 2 3 4 5 6 7 8 9 10 Mean
Statistic Critical
N n-P rametric Analysis if applicable):
Effluent %
# Younq
25
21
23
21
25
21
21
25
24
22
22.8
Adult
No Red
IMethod: Steel's Test
0.96%
(L)ive
Dead
L
L
L
L
L
L
L
L
L
L
-2.7%
1 Effluent % Rank Sum Critical
0.2% 100.50 75.00
1 2 3 4 5 6 7 8 9 10 Mean
0.5% 106.00 75.00
#Youn-q
211
221
211
201
201
161
201
171
21
25E86d-%/.
Effluent %
1.0% 114.50 75.00
1.9% 79.50 75.00
Adult
(L)ive
1.92%
D ead
L
L
L
L
L
L
L
L
L
L
3.8% 68.00 75.00
Overall Analysis IC25= >3.84%
1 2 3 4 5 6 7 8 9 10 Mean
Result = PASS/FAIL or
# Youna
22
13
20
12
21
1 21
20
19
20
20
18.8
Effluent %
Test LOEC: >3.84% NOEC= 3.84%
Adult
A Red
(L)ive
IL
�1-
LIL
�I- �I-
3.84%
(D)ead
L
L
L
L
153%
Chronic Value= >3.84%
Environmental Sciences Branch "Should use highest test concentration or
MAIL Division of Water Quality highest concentration with D.O. >5.0 mg/I
N.C. DENR % Reduction from Control Reproduction Mean
TO. 1621 Mail Service Center
Raleigh, NC 27699-1621 DWQ form AT-3 (8/91) Rev. 11/95
Page 2 of 5

Test Day
source ran 1 2 3 d 5 R 7 A
INS 1-31
t
----�.:-�-
----®-�-
+Client
'VENATOR CHEMIC
:Sample ID
45 EFFLUENT
NPDES#
0
County
0
Month
2
Start & fed Date
12-Fein-20
Start & Fed Time
01:50 PM
Started & Fed By
AM
"lest Organism
Csnodaphnla dubia
Neo. born date
11-Feb-20
Noo. born time
1645.2200
Test Type
INCCD
Dilution Water
M1HSF
Units for Conc.
%3rd BROOD
Test vessels
30 ml
Test volume
15 ml
Incubator 0
7-
Light
1611/8dk
Initial Temp °C
25
Setenastrum
0.05 ml
YAT
0.OS ml
Test method
I --PA 021-R-02-013:1002
N/A-Losl or not
Page 3 of 5

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