NC Coastal Reserve & National Estuarie 88533C

a�consrkl. NCAMA El DREDGE & FILL N? 88533 A B �) D
40', GENERAL PERMIT Previous permit
Date previous permit issued INNew ❑ Modification [-]Complete Reissue ❑ Partial Reissue
As authorized by the State of North Carolina, Department of Environmental Quality and the Coastal Resources Commission in an area of environmental concern pursuant to:
I SA NCAC _ ' C~IOL ❑ Rules attached. General Permit Rules available at the following link: www.den.nc.yov/CAMArules
City _ZIP
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Affected ❑CW EW PTA 1-1ES❑ n PTS Adj. Wtr. Body—�l�t'� ' R/ti,VL (nat/man/unk)
AEC(s): ❑ OEA ❑ IHA ❑ UW ❑ SPI MA ❑ PWS Closest Maj. Wtr. Body
ORW: yes/ 00
PNA: yes/nreA
Type of Project/ Activity
Shoreline Length
Access Length
Pier(dock)length
Fixed Platform(s) '�—
Floating Platforms)
Finger pier(s)
Total Platform area
Groin length/#
Bulkhead/ Riprap length -
Avg distance offshore---
Breakwater/Sill
Max distance/ length—
Basin, channel
Cubic yards
Boat ramp
Boathouse/ Boatlift
Beach Bulldozing'—
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(Scale: AJi�)
1/9

Styron, Heather M.
From: Lytle, Madison Ann <[e-mail]>
Sent: Thursday, May 26, 2022 6:45 AM
To: Styron, Heather M.
Subject: Re: [External] CAMA permitting for seagrass research
Attachments: NCNERR_03_Research_Permit_Lytle_2022_approved. pdf, Lytle_CAMA Proposal.docx
CAUTION: External email. Do not click links or open attachments unless you verify. Send all suspicious email as an attachment to
Resort Spam.
Attached is the NERR research permit and CAMA proposal document that has the specifics on the dimension
of the structures.
Thank you,
Madison
From: Styron, Heather M. <[e-mail]>
Sent: Tuesday, May 24, 2022 11:31 AM
To: Lytle, Madison Ann <[e-mail]>
Subject: RE: [External] CAMA permitting for seagrass research
I don't consider core sample as development so I think we can do this through the temporary structures general permit.
Because of where it is located there are not any adjacent neighbors you would need to notify. I know you have sent me
your reserve permit and proposal in previous emails but if you could resend all of that to me in one email I will draft the
general permit as soon as I can. The temporary GP would be valid for one year and the fee would be 200 dollars.
Heather Styron
Field Representative
(division of Coastal Management 400 Commerce Avenue Morehead City, 28557
252.515.5417 (0) 252.725.3903 (M)
Heather. M.Styron ncdenr.gov
www.deg.nc.gov
Find a Field Rep (arcgis.com)

From: Lytle, Madison Ann [mailto:[e-mail]]
Sent: Tuesday, May 24, 2022 5:32 AM
To: Styron, Heather M. <[e-mail]>
Subject: Re: [External] CAMA permitting for seagrass research
CAUTION: External email. Do not click links or open attachments unless you verify. Send all suspicious email as an attachment to
Re orp_ t Spam,
At the end of the experiment I will be collecting 22cm x 20 cm seagrass cores from each plot. These cores are
going to be used to answer the ecological questions outlined in the reserve permit.
Thank you,
Madison
From: Styron, Heather M. <[e-mail]>
Sent: Monday, May 23, 2022 9:24 AM
To: Lytle, Madison Ann <[e-mail]>
Subject: RE: [External] CAMA permitting for seagrass research
Hello Madison,
You are not going to removing any substrate etc. correct?
Heather
Heather Styron
Field Representative
Division of Coastal Management 400 Commerce Avenue Morehead City, 28557
252,515.5417 (0) 252.725,3903 (M)
[e-mail]
www.deg.nc.gov
Find a Field Rep (arcgis.coml
From: Lytle, Madison Ann [mailto:[e-mail]]
Sent: Sunday, May 22, 2022 6:18 AM
To: Styron, Heather M. <[e-mail]>
Subject: Re: [External] CAMA permitting for seagrass research
2

CAUTION: External email. Do not click links or open attachments unless you verify. Send all suspicious email as an attachment to
Report Spam.
Hi Heather,
Attached is the approved Reserve Permit. Please let me know if you need additional information to determine
if a CAMA permit is necessary.
Thank you,
Madison
From: Styron, Heather M. <[e-mail]>
Sent: Monday, April 4, 2022 1:57 PM
To: Lytle, Madison Ann <[e-mail]>
Subject: RE: [External] CAMA permitting for seagrass research
Afternoon Madison,
Before I continue looking over your project to determine permit type I would suggest first contacting Reserve
Staff (cc'd above) if you have not already. Once the Reserves component is signed off on then i can work with
you to determine the best application pathway. I suggest contacting Brandon at Brandon. Puckett(a:ncdenr.gov.
Hope this helps,
Heather
Heather Styron
Field Representative
Division of Coastal Management 400 Commerce Avenue Morehead City, 28557
252.515.5417 (0) 252.725,3903 (M)
Heather. [e-mail]
www.deg.nc.gov
Find a Field Rep (arcgis.com)
From: Lytle, Madison Ann [mailto:[e-mail]
Sent: Monday, April 4, 2022 1:51 PM

To: Styron, Heather M. <[e-mail]>
Subject: Re: [External] CAMA permitting for seagrass research
CAUTION: External email. Do not click links or open attachments unless you verify. Send all suspicious email as an attachment to
Report Spam.
Hi Heather,
I've pulled together a document with specifics on the project. Please let me know if you need additional
information to make a determination.
Thank you,
Madison
From: Styron, Heather M. <[e-mail]>
Sent: Wednesday, March 23, 2022 12:03 PM
To: Lytle, Madison Ann <[e-mail]>
Subject: RE: [External] CAMA permitting for seagrass research
Hello Madison,
You will need to provide sorne kind of plan and scope of project that describes the locations, how many plots, will the
total area exceed 100 square meters, what activity and/or structure(s) are needed and a possible sketch to make any
determination. We would need more information before a final determination could be made for permit type.
Heather
Heather Styron
Field Representative
Division of Coastal Management 400 Commerce Avenue Morehead City, 28557
252515.5417 (0) 252.7253903 (M)
HeatheL.M.Styron.Pncdenr.Rov_
www.deg.nc.gov
From: Lytle, Madison Ann [mailto:[e-mail]
Sent: Tuesday, March 22, 2022 1:32 PM

To: Styron, Heather M. <[e-mail]>
Subject: [External] CAMA permitting for seagrass research
CAUTION: External email. Do not click links or open attachments unless you verify. Send all suspicious email as an attachment to
Report Spam.
Hi Ms. Styron,
I'm a PhD student at UNC-Wilmington. I received a grant through the Coastal Reserve/ NC Sea Grant to do
seagrass physiology experiments at Rachel Carson Reserve (Middle Marsh). The proposed research involves
deploying shade plots, quarter meter plots with a cloth fixed 40cm above the sediment surface, and collecting
seagrass cores (-50). This work would happen in August -September 2022 and January -February 2023 with
shade plots being retrieved between events. Do I need a CAMA minor or general permit to conduct this
research?
Please let me know if you need more information.
Thank you,
Madison
Madison Lytle
Marine B3 olo,_;N [Phl-) Student
Coastal Plant Ecolo<)v Lab
l:) pt, c>I'I clog\ (I.L Marine Biolog
i. rl Le sit of ",ortll t arolina \N' l€11i11Wtorl

02 Research Permit
N.C. Coastal Reserve & National Estuarine Research Reserve
deq.nc.gov/coastaIreserve
Submit this form to research coordinator Brandon Puckett ([e-mail]).
Name: Madison Lytle
® Check if you are a student Advisor: Jessie Jarvis Degree: PhD Marine Blo.
Institution: University of North Carolina Wilmington
Email: mal6582Aunew.edu Phone: [phone]
Address 5600 Marvin K Moss Ln
Project Title: Effect of water clarity on Halodule wrightii seasonal persistence
Duration of field work: August - September 2022 and J
Project completed (final report submitted): May 2023
- February 2023 (8 weeks per time period)
Funding (source & annual amount): NC Coastal Reserve and NC Sea Grant (1 year $9,997)
Project Contact (if different from above):
Email:
Expected number of participants for field work at Reserve site: 44_6
How were you informed of the requirement to apply for a research permit?
Z Reserve staff ❑ Reserve website ❑ Colleagues ❑ Other:
Are you aware of the Reserve's long-term water quality and meteorological monitoring data? 0 yes ❑ no
If so, what data, if any, do you plan to use for your research (e.g., meteorological data from Masonboro
Island)? Water quality data from Rachel Carson Reserve
If not, please contact us or visit the NERRS Centralized Data Management Office.
0 I have attached a 1-2 page project description that includes project objectives, sampling locations (GPS
locations of sites within Reserve and list of sites outside of Reserve sites), and research methods.
Z I agree to (1) adhere to the research permit terms and conditions listed in the permit guidelines, including
submitting Final Report at the end of the project, and (2) notify Reserve staff of any permit deviations.
Signature: Madison Lytle
Typed name will serve as an official electronic signature.
Permit #: #03-2022
Approved:
Brandon Puckett, Research Coordinator
Date: 4/20/2022
Expiration Date: 05/01/2023
may be ren
Date: 05/09/2022

Project Overview. This project will quantify baseline light requirements for H. wrightii growth
and survival at the shallow and deep edge of meadows under thermally stressful and non -stressful
conditions using field and lab experiments. The field experiment will be conducted 2 times across
the Rachel Carson Reserve at Middle Marsh during optimal (August - September 2022) and
thermally stressful time periods (January - February 2023) and at 2 water depths shallow (<lm
MLW) and deep (>2m MLW). In the field at the beginning of each sampling period control and
light manipulated plots (0.25m2) will be established for each treatment. Plots will be deployed for
4 weeks, labeled with a buoy that complies with reserve regulations, and collected within one
month upon completion of each experimental period. Upon completion of the project a final report
will be submitted to the reserve within one year of permit expiration.
Project Objectives
Obj 1. Define specific light requirements for H. wrightii during thermally optimal and thermally
stressful time periods.
Obj 2. Determine if water depth, shallow (<_lm MLW) and deep (>2m MLW), influences light
requirements during thermally optimal and thermally stressful time periods.
Plot Design. For shaded treatments experimental plots will be covered with shade cloth fixed 0.4m
above the sediment surface (Fig 1). Vertical isolation borders (0.25m2 aluminum frames), to sever
root connection between experimental and non -experimental areas, will be inserted to ensure that
seagrass outside of the experimental plot does not translocate resources to SAV within treatment
plots (Fig 1). During each experimental period a total of 32 plots will be established (16 with shade
structures; 8 with no shade structure and only vertical isolation boarders; 8 with no shade structures
and no isolation boarders). The total area needed for the experiment is 8m2 (4m2 for shade plots;
4m2 for plots with no shade structure).
C . �m
S11hade Screen Support — Abo-, e sediment horizon
— • — Vertical Isolation Boarder — Belux sediment horizon
Figure 1. Shade plot design. A total of 32 plots will be established (16 with shade structures; 8 with
no shade structure and only vertical isolation boarders; 8 with no shade structures or isolation
boarders). Individual plot area is 0.25m2, total area need for the experiment is 8m2.

Plot Locations. Experiments will be
conducted 2 times across the Rachel
Carson Reserve at Middle Marsh
Sentinel Site 3 (Fig. 2) during August
- September 2022 and January -
February 2023. There will be 2 water
depth treatments - shallow (<1 m
MLW) and deep (>2m MLW).
Anticipated location of plot
placement is outline below (Fig 2;
Table 1). Experimental plots will be
established at locations where the
least amount of disturbance is
necessary to achieve seagrass cover
and water depth objectives.
Figure 2. Middle Marsh Sentinel Site 3 at Rachel Carson
Reserve. Dots indicate approximate locations of
experimental plots for shallow (pink) and deep (blue)
treatments.
Table 1. GPS coordinates for each experimental plots
Shallow Treatment GPS Coordinates
Deep Treatment GPS Coordinates
Plot Type
Latitude
Longitude
Latitude
Longitude
1 — Control 1
34.69735226
-76.59579304
34.69681517
-76.59636331
2 — Shade 17%
34.6973535
-76.59569774
34.69679245
-76.59618523
3 — Control 2
34.6973514
-76.59558505
34.69676281
-76.59598969
4 — Shade 22%
34.69736026
-76.59545524
34.69674372
-76.59580735
5 — Control 1
34.69736554
-76.59532535
34.6967314
-76.59565547
6 — Shade 17%
34.69736673
-76.59523439
34.69671856
-76.59554257
7 — Control 2
34.69737161
-76.59513483
34.69670573
-76.59542967
8 — Shade 22%
34.69738376
-76.59502674
34.69669965
-76.59534723
9 — Control 1
34.69921547
-76.59605027
34.69937303
-76.59567778
10 — Shade 17%
34.69914952
-76.59598546
34.69930715
-76.59560719
11 — Control 2
34,69908349
-76.59592642
34.69926021
-76.59554852
12 — Shade 22%
34.69902716
-76.59585601
34.69919441
-76.59547215
13 — Control 1
34.69896113
-76.59579697
34.69914739
-76.59541926
14 — Shade 17%
34.6988808
-76.59573766
34.69909098
-76.59535463
15 — Control 2
34.69882439
-76.59567303
34.69904396
-76.59530173
16 — Shade 22%
34.69877267
-76.59561426
34.69900186
-76.59523737
SAV Collection. Plots will be "gardened" to contain and maintain only the target species. Weekly
data will be collected on shoot density, canopy height (cm), leaf width (mm). At the initial week
and the final week C:N samples will be collected from all treatments. At the conclusion of each
experiment biomass cores (0.22 cm diameter, 10 cm depth) will be taken for all experimental plots.
For the lab component of this project H. wrightii will be collected from SAV meadows outside of
the reserve within Back Sound to alleviate harvesting pressure at the reserve site.

0)2 Research Permit
N.C. Coastal Reserve & National Estuarine Research Reserve
deq.nc.gov/coastal reserve
nomom
Submit this form to research coordinator Brandon Puckett (bran don. [e-mail]).
Name: Madison Lytle
® Check if you are a student Advisor: Jessie Jarvis Degree: PhD Marine Blo.
Institution: University of North Carolina Wilmington
Email: mal6582Auncw.edu Phone: [phone]
Address 5600 Marvin K Moss Ln
Project Title: Effect of water clarity on Halodule wrightii seasonal persistence
Duration of field work: August - September 2022 and January - February 2023 (8 weeks per time period)
Project completed (final report submitted): May 2023
Funding (source & annual amount): NC Coastal Reserve and NC Sea Grant (1 year $9,997)
Project Contact (if different from above):
Email:
Expected number of participants for field work at Reserve site: 44_6
How were you informed of the requirement to apply for a research permit?
Yl Reserve staff ❑ Reserve website ❑ Colleagues ❑ Other:
Are you aware of the Reserve's long-term water quality and meteorological monitoring data? 0 yes ❑ no
If so, what data, if any, do you plan to use for your research (e.g., meteorological data from Masonboro
Island)? Water quality data from Rachel Carson Reserve
If not, please contact us or visit the NERRS Centralized Data Management Office.
0 I have attached a 1-2 page project description that includes project objectives, sampling locations (GPS
locations of sites within Reserve and list of sites outside of Reserve sites), and research methods.
Z I agree to (1) adhere to the research permit terms and conditions listed in the permit guidelines including
submitting Final Report at the end of the project, and (2) notify Reserve staff of any permit deviations.
Signature: Madison Lytle
Typed name will serve as an official electronic signature.
Permit #: #03-2022
Approved:
Brandon Puckett Research Coordinator
Date: 4/20/2022
Expiration Date: 05/01/2023
may be renewed
Date: 05/09/2022

Project Overview. This project will quantify baseline light requirements for H. wrightii growth
and survival at the shallow and deep edge of meadows under thermally stressful and non -stressful
conditions using field and lab experiments. The field experiment will be conducted 2 times across
the Rachel Carson Reserve at Middle Marsh during optimal (August - September 2022) and
thermally stressful time periods (January - February 2023) and at 2 water depths shallow (<lm
MLW) and deep (>2m MLW). In the field at the beginning of each sampling period control and
light manipulated plots (0.25m2) will be established for each treatment. Plots will be deployed for
4 weeks, labeled with a buoy that complies with reserve regulations, and collected within one
month upon completion of each experimental period. Upon completion of the project a final report
will be submitted to the reserve within one year of permit expiration.
Project Objectives
Obj 1. Define specific light requirements for H. wrightii during thermally optimal and thermally
stressful time periods.
Obj 2. Determine if water depth, shallow (<lm MLW) and deep (>2m MLW), influences light
requirements during thermally optimal and thermally stressful time periods.
Plot Design. For shaded treatments experimental plots will be covered with shade cloth fixed 0.4m
above the sediment surface (Fig 1). Vertical isolation borders (0.25m2 aluminum frames), to sever
root connection between experimental and non -experimental areas, will be inserted to ensure that
seagrass outside of the experimental plot does not translocate resources to SAV within treatment
plots (Fig 1). During each experimental period a total of 32 plots will be established (16 with shade
structures; 8 with no shade structure and only vertical isolation boarders; 8 with no shade structures
and no isolation boarders). The total area needed for the experiment is 8m2 (4m2 for shade plots;
4m2 for plots with no shade structure).
0. �In
Shade Screen_ Support — Abo-, e sedirnent horizon
— • — Vertical Isolation Boarder — Below sediment horizon:
Figure 1. Shade plot design. A total of 32 plots will be established (16 with shade structures; 8 with
no shade structure and only vertical isolation boarders; 8 with no shade structures or isolation
boarders). Individual plot area is 0.25m2, total area need for the experiment is 8m2.

Plot Locations. Experiments will be
conducted 2 times across the Rachel
Carson Reserve at Middle Marsh
Sentinel Site 3 (Fig. 2) during August
- September 2022 and January -
February 2023. There will be 2 water
depth treatments - shallow (<1 m
MLW) and deep (>2m MLW).
Anticipated location of plot
placement is outline below (Fig 2;
Table 1). Experimental plots will be
established at locations where the
least amount of disturbance is
necessary to achieve seagrass cover
and water depth objectives.
Figure 2. Middle Marsh Sentinel Site 3 at Rachel Carson
Reserve. Dots indicate approximate locations of
experimental plots for shallow (pink) and deep (blue)
treatments.
Table 1. GPS coordinates for each experimental plots
Shallow Treatment GPS Coordinates
Deep Treatment GPS Coordinates
Plot Type
Latitude
Longitude
Latitude
Longitude
1 — Control 1
34.69735226
-76.59579304
34.69681517
-76.59636331
2 — Shade 17%
34.6973535
-76.59569774
34.69679245
-76.59618523
3 — Control 2
34.6973514
-76.59558505
34.69676281
-76.59598969
4 — Shade 22%
34.69736026
-76.59545524
34.69674372
-76.59580735
5 — Control 1
34.69736554
-76.59532535
34.6967314
-76.59565547
6 — Shade 17%
34.69736673
-76.59523439
34.69671856
-76.59554257
7 — Control 2
34.69737161
-76.59513483
34.69670573
-76.59542967
8 — Shade 22%
34.69738376
-76.59502674
34.69669965
-76.59534723
9 — Control 1
34.69921547
-76.59605027
34.69937303
-76.59567778
10 — Shade 17%
34.69914952
-76.59598546
34.69930715
-76.59560719
11 — Control 2
34.69908349
-76.59592642
34.69926021
-76.59554852
12 — Shade 22%
34.69902716
-76.59585601
34.69919441
-76.59547215
13 — Control 1
34.69896113
-76.59579697
34.69914739
-76.59541926
14 — Shade 17%
34.6988808
-76.59573766
34.69909098
-76.59535463
15 — Control 2
34.69882439
-76.59567303
34.69904396
-76.59530173
16 — Shade 22%
34.69877267
-76.59561426
34.69900186
-76.59523737
SAV Collection. Plots will be "gardened" to contain and maintain only the target species. Weekly
data will be collected on shoot density, canopy height (cm), leaf width (mm). At the initial week
and the final week C:N samples will be collected from all treatments. At the conclusion of each
experiment biomass cores (0.22 cm diameter, 10 cm depth) will be taken for all experimental plots.
For the lab component of this project H. wrightii will be collected from SAV meadows outside of
the reserve within Back Sound to alleviate harvesting pressure at the reserve site.

r
34.6966161
_7ti 507751 R
,F
34.6988541
-76.5951202

Figure 1. Middle Marsh at Rachel Carson Reserve (a). Study area delineated by black line and
GPS coordinates of boundary corners (b).
I1_4 ?II
-J/